##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003867_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1724759 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.483787590034318 31.0 31.0 34.0 30.0 34.0 2 31.686929014430422 31.0 31.0 34.0 30.0 34.0 3 31.76075034251162 31.0 31.0 34.0 30.0 34.0 4 35.4587968521979 37.0 35.0 37.0 33.0 37.0 5 35.33393361043485 37.0 35.0 37.0 33.0 37.0 6 35.386721275262225 37.0 35.0 37.0 33.0 37.0 7 35.32365101443158 37.0 35.0 37.0 32.0 37.0 8 35.33680937452711 37.0 35.0 37.0 32.0 37.0 9 36.850393011429425 39.0 37.0 39.0 33.0 39.0 10-11 36.766891200451774 39.0 36.0 39.0 32.0 39.0 12-13 36.71455838177972 39.0 35.0 39.0 32.0 39.0 14-15 37.654403020943796 39.0 36.5 41.0 32.0 41.0 16-17 37.765353594328246 39.0 37.0 41.0 32.0 41.0 18-19 37.797799866532074 39.5 37.0 41.0 32.0 41.0 20-21 37.6820854391831 39.5 36.5 41.0 32.0 41.0 22-23 37.604642735593785 39.0 36.5 41.0 32.0 41.0 24-25 37.422129120648165 39.0 36.0 41.0 31.5 41.0 26-27 37.11252151749896 39.0 36.0 40.5 31.0 41.0 28-29 37.019320380412566 39.0 36.0 40.0 31.0 41.0 30-31 36.877309815458275 39.0 36.0 40.0 30.0 41.0 32-33 36.64378704503064 38.0 35.0 40.0 30.0 41.0 34-35 36.55233223888091 38.0 35.0 40.0 30.0 41.0 36-37 36.337643693988554 38.0 35.0 40.0 30.0 41.0 38-39 36.10599364896777 38.0 35.0 40.0 30.0 41.0 40-41 36.505265373307225 39.0 35.0 40.0 30.0 41.0 42-43 36.38736716260068 39.0 35.0 40.0 30.0 41.0 44-45 36.26398731648885 39.0 35.0 40.0 30.0 41.0 46-47 36.0491083102045 38.0 35.0 40.0 29.5 41.0 48-49 35.76553159021057 38.0 35.0 40.0 28.0 41.0 50-51 35.477023166714886 38.0 34.0 40.0 28.0 41.0 52-53 35.43413282667318 38.0 34.0 40.0 27.5 41.0 54-55 35.2548959013984 38.0 34.0 40.0 27.0 41.0 56-57 34.88605828408491 38.0 33.5 40.0 26.5 41.0 58-59 34.55909927126051 38.0 33.0 40.0 26.0 41.0 60-61 33.93705903259528 37.0 32.0 40.0 23.5 41.0 62-63 33.855740135288464 37.0 32.0 39.5 24.5 41.0 64-65 33.38973473975204 36.0 32.0 39.0 22.5 40.5 66-67 32.92299271956256 36.0 31.0 39.0 22.0 40.0 68-69 32.566007192888975 35.0 31.0 38.5 22.0 40.0 70-71 31.984997904055003 35.0 31.0 37.5 21.5 39.5 72-73 31.5257360593567 34.5 30.5 37.0 20.5 39.0 74-75 31.20089125495214 34.0 30.0 36.5 20.0 39.0 76-77 30.267367788775125 33.5 29.0 35.0 20.0 37.0 78-79 30.646726586149136 34.0 30.0 36.0 20.0 37.0 80-81 30.34482730630772 34.0 30.0 35.0 18.0 37.0 82-83 29.963328209912227 34.0 29.5 35.0 15.0 36.5 84-85 29.649230124324617 34.0 29.0 35.0 12.0 36.0 86-87 29.503915909411113 34.0 29.0 35.0 9.0 36.0 88-89 29.1795987149509 34.0 29.0 35.0 7.0 35.0 90-91 28.804196412368334 34.0 29.0 35.0 2.0 35.0 92-93 28.518695945346565 34.0 29.0 35.0 2.0 35.0 94-95 28.06507169987227 33.0 27.5 35.0 2.0 35.0 96-97 27.558959251698354 33.0 27.0 35.0 2.0 35.0 98-99 27.24241966558806 33.0 27.0 35.0 2.0 35.0 100-101 26.152215468943776 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19042904093211277 1101 2 0.2526570540178703 1101 3 0.2420121719785513 1101 4 0.24376605126522577 1101 5 0.27579108906141414 1101 6 0.2856756497864126 1101 7 0.22289154871501182 1101 8 0.2929065089676115 1101 9 0.39447990959779844 1101 10-11 0.4738448780320965 1101 12-13 0.4085865885007749 1101 14-15 0.38759773529115193 1101 16-17 0.4270208101017161 1101 18-19 0.3463798983864024 1101 20-21 0.33850396464396226 1101 22-23 0.4026589085506984 1101 24-25 0.4449322401460165 1101 26-27 0.4601315361204925 1101 28-29 0.3484988558667439 1101 30-31 0.47714315305989174 1101 32-33 0.6267145303290036 1101 34-35 0.5265598938091642 1101 36-37 0.4852578150249869 1101 38-39 0.5124683859442953 1101 40-41 0.5150386656546218 1101 42-43 0.3603278368698568 1101 44-45 0.46362753437525583 1101 46-47 0.5495490225304778 1101 48-49 0.5670943935367418 1101 50-51 0.5629822574144967 1101 52-53 0.6652604151663795 1101 54-55 0.7711497593442687 1101 56-57 0.6642820908432441 1101 58-59 0.6544036532041204 1101 60-61 0.7059132286384155 1101 62-63 0.5655315040754729 1101 64-65 0.42717509658628927 1101 66-67 0.5291010794710544 1101 68-69 0.45145094644354344 1101 70-71 0.6283642135578837 1101 72-73 0.5708571281539996 1101 74-75 0.44081609061814575 1101 76-77 0.42061070300126957 1101 78-79 0.5136005115905036 1101 80-81 0.6575088949991788 1101 82-83 0.6522615146861348 1101 84-85 0.32867762232498166 1101 86-87 0.48480195557169026 1101 88-89 0.5744718325489053 1101 90-91 0.6699642929632112 1101 92-93 0.8318635752160439 1101 94-95 0.6834075067478125 1101 96-97 0.73917343715814 1101 98-99 0.6670354198077817 1101 100-101 0.79451371064334 1102 1 0.14430357666334714 1102 2 0.2243636761833514 1102 3 0.19814533900421694 1102 4 0.17811422077815564 1102 5 0.020918217223687918 1102 6 0.15759767142111514 1102 7 0.1637238385412516 1102 8 0.16831592724201982 1102 9 0.2038662457402296 1102 10-11 0.18658097406579088 1102 12-13 0.37469241604886605 1102 14-15 0.4633896366958652 1102 16-17 0.33405204670051347 1102 18-19 0.21842060619052717 1102 20-21 0.29630396578752993 1102 22-23 0.13600094118000072 1102 24-25 0.34481098094538254 1102 26-27 0.25487895839248864 1102 28-29 0.3370529920348204 1102 30-31 0.4452470414409859 1102 32-33 0.44934601426552945 1102 34-35 0.3884279389670908 1102 36-37 0.3770368578083705 1102 38-39 0.3396234629432726 1102 40-41 0.4941619073145276 1102 42-43 0.634684027705454 1102 44-45 0.764915316071118 1102 46-47 0.7631425505810157 1102 48-49 0.7830207623689276 1102 50-51 0.9597484881361567 1102 52-53 1.0637830051550807 1102 54-55 0.9389150694925519 1102 56-57 1.0859713961003123 1102 58-59 0.8574247185697246 1102 60-61 0.7635482290509188 1102 62-63 0.6288665037528176 1102 64-65 0.7293279068673044 1102 66-67 0.7351398203357746 1102 68-69 0.7656339436899913 1102 70-71 0.8886500078934567 1102 72-73 0.9880790386174141 1102 74-75 1.0372543211241698 1102 76-77 1.0065541110178557 1102 78-79 0.9820800216853627 1102 80-81 1.021998123800465 1102 82-83 1.0469965619033026 1102 84-85 0.8948769366734304 1102 86-87 0.8394360664505491 1102 88-89 1.0464530339012583 1102 90-91 1.235714307053616 1102 92-93 1.3619498656973796 1102 94-95 1.4118678457181275 1102 96-97 1.5149515748880056 1102 98-99 1.4313239113335214 1102 100-101 1.389116634750355 1103 1 0.14012127038706268 1103 2 0.09559954258875081 1103 3 0.10370161308944503 1103 4 0.09392136370482973 1103 5 0.1153743667483198 1103 6 0.16501901675898978 1103 7 0.21892926153967096 1103 8 0.1612991836463209 1103 9 0.11983823328138499 1103 10-11 0.18796838533317128 1103 12-13 0.233052369747746 1103 14-15 0.32800172570269126 1103 16-17 0.11348346257945963 1103 18-19 0.2532461170535356 1103 20-21 0.28875972869684574 1103 22-23 0.31554607017461933 1103 24-25 0.22886189364031395 1103 26-27 0.11642649515687253 1103 28-29 0.304747805131349 1103 30-31 0.415098046200427 1103 32-33 0.35141269103797157 1103 34-35 0.40379589801368354 1103 36-37 0.34512598144785755 1103 38-39 0.34143629013502874 1103 40-41 0.4098915417498077 1103 42-43 0.5881895949197329 1103 44-45 0.6099235513483379 1103 46-47 0.7203133608460703 1103 48-49 0.6643448317686946 1103 50-51 0.7778368932459685 1103 52-53 0.8254956646744702 1103 54-55 0.7521791736506174 1103 56-57 0.6219390910061193 1103 58-59 0.7427892889376579 1103 60-61 0.7695724745599932 1103 62-63 0.43710335709349835 1103 64-65 0.650987497175592 1103 66-67 0.6915992398657806 1103 68-69 0.7350807639180417 1103 70-71 0.8173410368882195 1103 72-73 0.925794104979424 1103 74-75 0.661430851349003 1103 76-77 0.7641405559323573 1103 78-79 0.7574746914931616 1103 80-81 0.7999435739546712 1103 82-83 0.7700047628765141 1103 84-85 0.7034190955908706 1103 86-87 0.6431753412362298 1103 88-89 0.7051069821291094 1103 90-91 0.6707690057045887 1103 92-93 0.5848069436550709 1103 94-95 0.46766357561216054 1103 96-97 0.871081907826845 1103 98-99 0.9618018540949862 1103 100-101 1.0344482547380913 1104 1 0.08360699931052551 1104 2 0.10118857216620114 1104 3 0.07090728254102885 1104 4 0.1736423661674351 1104 5 0.17061338413927274 1104 6 0.19842899849922446 1104 7 0.18856934477189213 1104 8 0.12326388231574725 1104 9 0.06102395505721603 1104 10-11 0.12534416186166197 1104 12-13 0.0572516014434683 1104 14-15 0.1984268502202795 1104 16-17 0.24894342849278672 1104 18-19 0.1467440657708039 1104 20-21 0.11638161253844004 1104 22-23 0.1637704042790915 1104 24-25 0.1968590749856105 1104 26-27 0.4423422693679342 1104 28-29 0.3563394748945825 1104 30-31 0.41466158535460096 1104 32-33 0.5146943860310245 1104 34-35 0.5374637939849407 1104 36-37 0.39282518628446184 1104 38-39 0.4185990160569375 1104 40-41 0.4704637055700758 1104 42-43 0.5735540888382502 1104 44-45 0.7593697427460384 1104 46-47 0.7685219457340153 1104 48-49 0.6981613075579602 1104 50-51 0.7502360860514514 1104 52-53 0.9656448238505462 1104 54-55 0.9037094447768723 1104 56-57 0.763065500721936 1104 58-59 0.8662140898138446 1104 60-61 0.8590919536438335 1104 62-63 0.8445527365144301 1104 64-65 0.8861968296796263 1104 66-67 0.9640081885284388 1104 68-69 0.8581264293207695 1104 70-71 0.9036712163826728 1104 72-73 0.8343551415731021 1104 74-75 0.8877575813321847 1104 76-77 0.8653378737486612 1104 78-79 1.00755804358349 1104 80-81 1.0142233741582665 1104 82-83 0.882736189788826 1104 84-85 0.9156433400169348 1104 86-87 0.9017729262231775 1104 88-89 0.9714416610826042 1104 90-91 0.9591171270971977 1104 92-93 0.8779385427321778 1104 94-95 0.876792982451601 1104 96-97 1.1153589710629994 1104 98-99 1.2812637236768225 1104 100-101 1.4200430157428983 1105 1 -0.06320095654737301 1105 2 0.02445218266009519 1105 3 -0.02838840055919789 1105 4 0.07897174496639536 1105 5 -0.08068753193106915 1105 6 0.04799181555642207 1105 7 -0.07617092663019776 1105 8 0.08344119764578295 1105 9 0.21299481002864695 1105 10-11 0.08316602070823365 1105 12-13 -0.030200391466017606 1105 14-15 0.0652787948878597 1105 16-17 0.12653511630599468 1105 18-19 0.04964040276686177 1105 20-21 0.046355319944474616 1105 22-23 0.05608320198692951 1105 24-25 0.08843847538823013 1105 26-27 0.07168169330538632 1105 28-29 0.06156269518503876 1105 30-31 0.28212528932659353 1105 32-33 0.2537658456795242 1105 34-35 0.13631237524438689 1105 36-37 0.1658660536470009 1105 38-39 0.3740935971074819 1105 40-41 0.42638218800873773 1105 42-43 0.5611549967179883 1105 44-45 0.5832262703953504 1105 46-47 0.4298937744106013 1105 48-49 0.6745401470919816 1105 50-51 0.5981268737246666 1105 52-53 0.4862265371598298 1105 54-55 0.5845148931073041 1105 56-57 0.5539245975161009 1105 58-59 0.506384649773203 1105 60-61 0.4748165629557235 1105 62-63 0.4610908645328422 1105 64-65 0.39350459655245373 1105 66-67 0.5598099022435008 1105 68-69 0.39043686377602427 1105 70-71 0.5098717111252817 1105 72-73 0.48583146057988813 1105 74-75 0.5322624930652644 1105 76-77 0.5413537905741954 1105 78-79 0.553377716515346 1105 80-81 0.6641037462371706 1105 82-83 0.4822424616132217 1105 84-85 0.4951124047024287 1105 86-87 0.7802726252747938 1105 88-89 0.675991128956273 1105 90-91 0.7566197251721825 1105 92-93 0.8083402844004937 1105 94-95 0.8626562046056137 1105 96-97 0.852010445033784 1105 98-99 0.9726634191967136 1105 100-101 1.073306145320526 1106 1 -0.1118689954582841 1106 2 -0.13176557964269264 1106 3 -0.06984391855447925 1106 4 -0.2548234903887874 1106 5 -0.07912509907190213 1106 6 -0.04536516319726758 1106 7 -0.025724311852926007 1106 8 -0.05592387177036784 1106 9 0.06371203972792472 1106 10-11 -0.28029072306667047 1106 12-13 -0.32560213476114797 1106 14-15 -0.23729476077446066 1106 16-17 -0.05460774711391991 1106 18-19 -0.1426640338510552 1106 20-21 -0.17179791413969525 1106 22-23 -0.08554458424666223 1106 24-25 -0.08791014874360314 1106 26-27 -0.15678178838044943 1106 28-29 -0.1329461416392661 1106 30-31 -0.24477288405498854 1106 32-33 -0.1450488570850723 1106 34-35 -0.18209099025408193 1106 36-37 -0.2314358096091027 1106 38-39 0.2306050102013515 1106 40-41 -0.19453253953637528 1106 42-43 -0.14249694777752353 1106 44-45 -0.03904379334584007 1106 46-47 0.02759719841953512 1106 48-49 0.055809465479036646 1106 50-51 0.20889515711302664 1106 52-53 0.314246489517906 1106 54-55 0.28769788158646037 1106 56-57 0.2945306961089784 1106 58-59 0.2601276554145713 1106 60-61 0.4293110290162616 1106 62-63 0.41599290070335115 1106 64-65 0.5198416381330091 1106 66-67 0.179998176049196 1106 68-69 0.28263125807966816 1106 70-71 0.4178451911213905 1106 72-73 0.4795024120905147 1106 74-75 0.24188050149322393 1106 76-77 0.12403656791814299 1106 78-79 0.4124014285986455 1106 80-81 0.381237489649898 1106 82-83 0.49900210638913833 1106 84-85 0.4600159819822771 1106 86-87 0.5746175617290739 1106 88-89 0.44276248551695474 1106 90-91 0.4692411198405928 1106 92-93 0.5257287793246874 1106 94-95 0.622393705546596 1106 96-97 0.4571069937565859 1106 98-99 0.4628148582277447 1106 100-101 0.40591469418031423 1107 1 -0.23195780990731762 1107 2 -0.05915005004024465 1107 3 -0.18788609533354972 1107 4 -0.19384423041446297 1107 5 -0.2670683175964257 1107 6 -0.22083466371225313 1107 7 -0.3011801986750129 1107 8 -0.16387816459861426 1107 9 -0.38254389678184 1107 10-11 -0.1631490210250277 1107 12-13 -0.25330471636652874 1107 14-15 -0.33794441353362004 1107 16-17 -0.26330634682872045 1107 18-19 -0.1490704910502032 1107 20-21 -0.06029030995254203 1107 22-23 -0.17154439081305384 1107 24-25 -0.15259260821078158 1107 26-27 -0.20159375669583568 1107 28-29 -0.24420095284209964 1107 30-31 -0.05417087005351107 1107 32-33 -0.028217473901783308 1107 34-35 -0.18440715154105192 1107 36-37 -0.399441156077593 1107 38-39 -0.23574258267313297 1107 40-41 -0.17350763472909136 1107 42-43 -0.08353682306326249 1107 44-45 -0.1771579164292021 1107 46-47 -0.0579708203114464 1107 48-49 -0.25554279592953577 1107 50-51 0.04783554426445136 1107 52-53 -0.24419636070351913 1107 54-55 -0.08308662923963084 1107 56-57 0.058147253843173985 1107 58-59 -0.16755911230511344 1107 60-61 -0.2765605521476999 1107 62-63 -0.09735241451323162 1107 64-65 -0.2172726806758405 1107 66-67 -0.30795966458993007 1107 68-69 -0.12012319994638432 1107 70-71 -0.0661465728235946 1107 72-73 -0.14374803356311716 1107 74-75 -0.028306611152519423 1107 76-77 -0.24605281949776625 1107 78-79 -0.16141297688863787 1107 80-81 -0.0644548766437012 1107 82-83 -0.04981948410273063 1107 84-85 -0.17389210588059356 1107 86-87 -0.11177681274465812 1107 88-89 -0.35490984802126846 1107 90-91 -0.05228937093533048 1107 92-93 -0.1050729113401232 1107 94-95 0.0893245786976884 1107 96-97 0.0369852222530227 1107 98-99 -0.25800290759565314 1107 100-101 -0.18057190504127263 1108 1 -0.3361250970014318 1108 2 -0.28749075050637884 1108 3 -0.32075896514021807 1108 4 -0.24723289878571109 1108 5 -0.18214279016218882 1108 6 -0.21160270326078034 1108 7 -0.1880121046706833 1108 8 -0.2854791084992101 1108 9 -0.33245164808332106 1108 10-11 -0.2896343243396018 1108 12-13 -0.41320198776205785 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-0.9086307423999358 2316 48-49 -1.0153834843416547 2316 50-51 -1.0078072256640311 2316 52-53 -1.1652092102877916 2316 54-55 -1.283737829890839 2316 56-57 -1.5268286168469842 2316 58-59 -1.350651152540017 2316 60-61 -1.4067134680149067 2316 62-63 -1.300183974487645 2316 64-65 -1.0932829138289364 2316 66-67 -1.1154484720787323 2316 68-69 -1.0867491665723534 2316 70-71 -1.6809497590553484 2316 72-73 -1.3687320442614173 2316 74-75 -1.1173524859126829 2316 76-77 -1.3255632561510602 2316 78-79 -1.3136020576491454 2316 80-81 -1.3670015363332162 2316 82-83 -1.173576679288498 2316 84-85 -1.2607866927751807 2316 86-87 -1.057558544240674 2316 88-89 -1.2003659791916839 2316 90-91 -1.57473253623564 2316 92-93 -1.5067241701342375 2316 94-95 -1.2907847299830486 2316 96-97 -0.9431008292615992 2316 98-99 -1.533629397507859 2316 100-101 -2.0705655093205912 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 10.0 5 33.0 6 91.0 7 246.0 8 625.0 9 1063.0 10 1778.0 11 2750.0 12 4111.0 13 5535.0 14 6909.0 15 8648.0 16 10554.0 17 12617.0 18 12022.0 19 10505.0 20 10700.0 21 11353.0 22 12111.0 23 13513.0 24 15930.0 25 19464.0 26 24241.0 27 30296.0 28 38212.0 29 47050.0 30 57644.0 31 69614.0 32 85236.0 33 104088.0 34 132587.0 35 172279.0 36 239168.0 37 294560.0 38 234095.0 39 35108.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.09347561587659 19.02288283447992 14.243126997729643 19.640514551913846 2 14.790233347209933 25.34211231233251 35.37906890717714 24.488585433280416 3 27.368773280335983 24.08524191252074 28.668731583779056 19.87725322336422 4 12.40022519088174 16.384318040955286 42.26074483449572 28.954711933667255 5 12.552826220938693 41.04637227577882 33.80460690450086 12.596194598781626 6 27.87712367065841 39.20390833094648 15.536438831443371 17.382529166951734 7 27.314656714358353 33.023918124213296 21.72871688160491 17.93270827982344 8 23.918588046213994 29.362189152223582 19.22396114471645 27.49526165684597 9 20.996904495062786 23.69287535244054 24.74450053601692 30.565719616479754 10-11 26.766799023974773 24.039853880314862 27.167146784418968 22.0262003112914 12-13 31.267875685820457 22.519204132287467 25.458107480523367 20.754812701368714 14-15 22.938978114547908 26.095845356063673 29.8565621712261 21.10861435816232 16-17 20.014477384956393 29.123402168071017 28.906792195315408 21.95532825165719 18-19 19.532612962158773 33.77277637049582 26.813572215016706 19.881038452328703 20-21 23.131008848736474 26.5464993563501 24.84268268639753 25.479809108515894 22-23 20.65326228186083 28.15338258852396 26.469263241994966 24.724091887620244 24-25 25.959771771012647 27.21522253253933 25.899357533429306 20.925648163018717 26-27 24.046172222447716 24.28219386187182 31.904118564561674 19.767515351118792 28-29 20.353018124635426 32.65913415987578 27.015001617753303 19.972846097735495 30-31 22.390580248592233 26.73895843836785 26.29976316679824 24.570698146241675 32-33 23.105403045409794 27.712510161222802 27.8503607016665 21.331726091700904 34-35 26.271193565604058 25.234820488691017 27.645747574099023 20.848238371605905 36-37 23.96915631014644 24.50130100432779 31.62855471089985 19.900987974625924 38-39 21.722884772332254 28.58662043392482 29.74581172817583 19.94468306556709 40-41 20.964176785072308 29.82056500713217 27.452075287896182 21.763182919899336 42-43 20.37757159116143 29.964534175499303 24.246141055069142 25.411753178270125 44-45 23.288152141835468 25.68295048757537 25.36905156024697 25.659845810342198 46-47 25.997314404289995 28.634874362011576 26.088602626340986 19.279208607357443 48-49 24.636166560081726 27.659168614281764 26.948924458431584 20.755740367204925 50-51 19.172678617708332 28.86110465288194 31.91605319931654 20.05016353009319 52-53 22.46974794739439 31.049294423162888 24.756676150117205 21.724281479325516 54-55 19.880226744722016 27.35840195644725 27.3769552731715 25.384416025659235 56-57 24.880684050089073 24.789858108019963 27.238215892075708 23.09124194981526 58-59 28.276438263079832 25.011247980871477 25.6351920331827 21.077121722865986 60-61 22.59989852866565 27.124795245343194 31.021468435167066 19.25383779082409 62-63 20.35646803579293 27.902376907801862 29.499652224185073 22.24150283222013 64-65 19.74936569849453 32.61723809156393 26.658415260016483 20.97498094992506 66-67 22.313495287688486 26.31216026324687 24.825365232414303 26.54897921665034 68-69 20.310350205742466 28.33155237721404 26.380898100513186 24.977199316530307 70-71 25.217842281550386 27.226965885629106 25.467732555948462 22.087459276872046 72-73 23.16517843942255 24.41601986132555 30.97577690564305 21.44302479360885 74-75 20.148061265370988 32.00597880631439 26.648360727498737 21.19759920081588 76-77 21.829339635276582 26.641490202399293 25.980615262770044 25.548554899554084 78-79 22.36190679393469 27.537383483721495 27.357967113086524 22.742742609257295 80-81 25.045615068540013 25.770440971753157 26.48946896348997 22.69447499621686 82-83 22.88479144042733 24.969314553511534 30.477620351596947 21.66827365446419 84-85 21.074944383534163 28.073197472806342 28.29885218746503 22.55300595619446 86-87 20.706847134081098 28.878294076771414 26.195460368454786 24.219398420692702 88-89 20.45424897043587 28.80576358784039 24.033647599461723 26.70633984226202 90-91 22.715724341777605 24.622686415899263 25.49460533326685 27.16698390905628 92-93 24.47785458722059 27.807160304715033 26.106313983576833 21.60867112448754 94-95 23.291738113908767 27.62762301367004 26.343993286012672 22.736645586408525 96-97 18.88828236134476 28.535046581991814 30.485266163967108 22.091404892696325 98-99 21.75903378348605 30.108601372490355 24.292650288621875 23.839714555401716 100-101 19.039626098973493 26.742956669562762 26.490251108214046 27.727166123249695 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 49.0 1 32.5 2 42.0 3 66.0 4 68.0 5 66.5 6 78.0 7 142.0 8 234.0 9 296.0 10 333.5 11 380.5 12 426.0 13 479.5 14 576.0 15 678.5 16 746.5 17 868.0 18 1029.5 19 1183.0 20 1304.5 21 1463.5 22 1716.5 23 2020.0 24 2439.0 25 3018.0 26 3878.5 27 5185.0 28 6915.0 29 9137.5 30 11940.5 31 14979.0 32 18148.0 33 21512.5 34 25091.0 35 28864.0 36 32495.0 37 37229.0 38 41656.0 39 44463.5 40 46765.5 41 48914.5 42 51247.0 43 54170.0 44 56185.5 45 58525.0 46 67878.0 47 122505.0 48 236144.5 49 227645.5 50 114538.0 51 65777.0 52 49470.5 53 38930.0 54 31766.5 55 26978.0 56 22707.0 57 18633.5 58 15322.5 59 12856.0 60 10485.0 61 8156.5 62 6061.0 63 4343.5 64 2973.5 65 1915.0 66 1147.0 67 642.0 68 362.0 69 216.0 70 115.5 71 61.0 72 35.5 73 23.5 74 18.0 75 15.0 76 11.0 77 6.0 78 2.5 79 1.0 80 1.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07189410230646717 2 0.0019133107871882392 3 0.02295972944625887 4 0.0 5 0.0 6 0.0019133107871882392 7 0.0 8 0.0 9 0.0 10-11 4.928224754878798E-4 12-13 0.0 14-15 0.004319444049864358 16-17 0.0 18-19 0.0 20-21 0.012697426133158314 22-23 0.0 24-25 0.0 26-27 0.007479305804463117 28-29 0.008406971640675597 30-31 0.007392337132318196 32-33 0.003768642459613198 34-35 0.005247109886076838 36-37 0.018669274953776148 38-39 0.008783835886636915 40-41 0.010378261542627115 42-43 0.0 44-45 0.0 46-47 1.1595822952655994E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.001826342115043319 58-59 2.8989557381639986E-4 60-61 0.007769201378279516 62-63 0.013248227723409474 64-65 0.058703853697820975 66-67 0.025858685184422865 68-69 0.0017393734428983992 70-71 1.1595822952655994E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 8.986762788308394E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.696867214491996E-5 96-97 2.6090601643475984E-4 98-99 6.377702623960797E-4 100-101 0.0014204883117003594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1724759.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.38611375555932 #Duplication Level Percentage of deduplicated Percentage of total 1 80.01569895350764 35.51585915980979 2 11.982919305580172 10.637504388423388 3 3.2679839292680715 4.35159319307497 4 1.4084710714496917 2.5006622879512213 5 0.7928592840029793 1.7595971185953783 6 0.5020135764858334 1.336945902764123 7 0.35492610496443044 1.1027653328838158 8 0.24712038795485836 0.8774970920865834 9 0.1830812554470476 0.7313638887704942 >10 1.1231399816532437 9.11755827032808 >50 0.07641890120282112 2.306887917675534 >100 0.03611133122287981 2.9978849253065114 >500 0.002998395732219852 0.9160445300942146 >1k 0.003780585923233727 4.0402939340091235 >5k 9.125552228495203E-4 3.3650792525822157 >10k+ 0.001564380382027749 18.442462805644507 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 92518 5.364111739669136 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 38068 2.207148940808542 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 28064 1.6271258767166892 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 22824 1.3233153153571022 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 20383 1.1817882962199358 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 19625 1.1378401272293694 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 18544 1.0751647041702637 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 17538 1.0168377147184042 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 17469 1.0128371557997378 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 16762 0.9718459216620989 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 13790 0.7995319925856307 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 13136 0.7616136515304457 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 9901 0.574051215271235 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 9533 0.552714901038348 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 8663 0.5022730711942944 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 8550 0.4957214312260438 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8511 0.49346024575027586 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6611 0.3832999277000439 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6386 0.3702546268783059 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 4858 0.2816625395200141 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4629 0.268385322239223 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4452 0.25812301892612244 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3984 0.2309887932169074 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3713 0.21527645311605853 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3589 0.20808704288541183 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3567 0.20681150236061965 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3212 0.18622891661965527 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3092 0.17927142284806166 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2857 0.16564633087869088 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2505 0.14523768248201632 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2464 0.14286053877672183 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2428 0.14077329064524377 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2401 0.1392078545466352 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2052 0.1189731434942505 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1954 0.11329119024744906 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1865 0.10813104903351714 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 1765 0.10233313755718915 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7393734428983992E-4 0.0 12-13 0.0 0.0 0.0 2.8989557381639986E-4 0.0 14-15 0.0 0.0 0.0 4.0585380334295983E-4 0.0 16-17 0.0 0.0 0.0 6.087807050144397E-4 0.0 18-19 0.0 0.0 0.0 8.696867214491996E-4 0.0 20-21 0.0 0.0 0.0 0.0011885718526472395 0.0 22-23 0.0 0.0 0.0 0.002493101934821039 0.0 24-25 0.0 0.0 0.0 0.004058538033429598 0.0 26-27 0.0 0.0 0.0 0.005971848820617838 0.0 28-29 0.0 0.0 0.0 0.013045300821737993 0.0 30-31 0.0 0.0 0.0 0.027018267479688468 0.0 32-33 0.0 0.0 0.0 0.049282247548787975 0.0 34-35 0.0 0.0 0.0 0.06760364781398445 0.0 36-37 0.0 0.0 0.0 0.08204044739004115 0.0 38-39 0.0 0.0 0.0 0.10111557614716027 0.0 40-41 0.0 0.0 0.0 0.1304240186599983 0.0 42-43 0.0 0.0 0.0 0.1882002065216068 0.0 44-45 0.0 0.0 0.0 0.2622975151890786 0.0 46-47 0.0 0.0 0.0 0.344801795497226 0.0 48-49 0.0 0.0 0.0 0.5077520975394243 0.0 50-51 0.0 0.0 0.0 0.644263923249567 0.0 52-53 0.0 0.0 0.0 0.7867186082229459 0.0 54-55 0.0 0.0 0.0 1.0904132113530065 0.0 56-57 0.0 0.0 0.0 1.4161688676504949 0.0 58-59 0.0 0.0 0.0 1.7964828709402298 0.0 60-61 0.0 2.8989557381639985E-5 0.0 2.173086210885115 0.0 62-63 0.0 5.797911476327997E-5 0.0 2.740614775745481 0.0 64-65 0.0 5.797911476327997E-5 0.0 3.0819088347995285 0.0 66-67 0.0 1.1595822952655994E-4 0.0 3.7584091458574793 0.0 68-69 0.0 1.1595822952655994E-4 0.0 4.546258346818309 0.0 70-71 0.0 1.1595822952655994E-4 0.0 4.828616635715482 0.0 72-73 0.0 1.7393734428983992E-4 0.0 5.277664879557086 0.0 74-75 0.0 1.7393734428983992E-4 0.0 5.927262881364875 0.0 76-77 0.0 2.3191645905311988E-4 0.0 6.1823999758806885 0.0 78-79 0.0 2.8989557381639986E-4 0.0 6.454727877923814 0.0 80-81 0.0 2.8989557381639986E-4 0.0 6.967205273316446 0.0 82-83 0.0 2.8989557381639986E-4 0.0 7.762939633885082 0.0 84-85 0.0 2.8989557381639986E-4 0.0 8.896779202195784 0.0 86-87 0.0 2.8989557381639986E-4 0.0 9.961449686593895 0.0 88-89 0.0 2.8989557381639986E-4 0.0 10.951298123389993 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1215 0.0 34.799095 2 AGTACGC 890 0.0 31.493156 2 ACCTGGG 1305 0.0 31.30614 3 GAGTACG 985 0.0 30.403463 1 GTACGCA 930 0.0 30.137737 3 TACGCAG 1010 0.0 28.212759 4 TATAACG 230 5.456968E-12 26.851572 2 CGCAGTG 1040 0.0 26.028984 6 GTACCTG 1745 0.0 24.516916 1 ACGCAGT 1110 0.0 24.387514 5 TTGTACG 190 3.290006E-7 22.499224 9 GTACCCG 150 3.0168596E-5 22.183268 1 GTACCGG 110 0.002865468 21.60708 1 TAACGCA 220 5.6541467E-8 21.587036 4 CCGGTCG 155 3.9089195E-5 21.450872 9 GTACACG 165 6.288736E-5 20.166609 1 ATAACGC 240 1.4125544E-7 19.793852 3 CCTGGGG 1325 0.0 19.713444 4 TACCGGG 125 0.0060274834 19.002651 2 GCATCGT 400 1.8189894E-12 18.99604 8 >>END_MODULE