FastQCFastQC Report
Wed 25 May 2016
SRR1003866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3893287
Sequences flagged as poor quality0
Sequence length121
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT210010.5394156659912305No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT151210.3883864713800961No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95420.2450885331597696No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC91570.23519971684594537No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT85300.21909507313486007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72500.1862179695460417No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN62780.16125191900828273No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA61320.15750187438018312No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT59870.1537775149892623No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT57090.14663701905356577No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA50950.13086628342580447No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50160.1288371496886821No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC47830.12285248942602998No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43050.11057494605458063No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC41540.10669647524058719No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG39100.10042927736896869No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG33500.028.329452
TAACGCA6150.026.1555354
GTATAAC13600.025.4350661
TATAACG6650.025.0827622
ACCTGGG37250.024.8308473
GAGTACT115150.024.52652412-13
ATAACGC8900.023.2383063
GTACATG145400.023.0373341
AGTACTT125050.022.584812-13
GTACTTT131400.021.756614-15
TACATGG141550.021.4140972
ACGCGAT2154.663034E-621.3660358
GTACACG5500.020.964661
ACATGGG141650.020.6439693
GTACCTG51050.020.1022551
CATGGGG63650.019.5858334
GTACTAG12100.018.5823141
CCGGTCG4951.8189894E-1218.5596719
GTCGCCG12900.018.047237114-115
TAACGCC4551.8007995E-1017.6765424
Produced by FastQC (version 0.11.5)