##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003866_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3893287 Sequences flagged as poor quality 0 Sequence length 121 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.63111607235737 31.0 31.0 34.0 27.0 34.0 2 30.575789044064823 31.0 31.0 34.0 27.0 34.0 3 30.624108625950257 31.0 31.0 34.0 26.0 34.0 4 34.14191273337928 37.0 35.0 37.0 30.0 37.0 5 34.09213423002209 37.0 35.0 37.0 30.0 37.0 6 34.188927248363655 37.0 35.0 37.0 30.0 37.0 7 34.20186336121637 37.0 35.0 37.0 30.0 37.0 8 34.16788102187175 37.0 35.0 37.0 30.0 37.0 9 35.60337858472802 39.0 35.0 39.0 30.0 39.0 10-11 35.451358196814155 38.0 35.0 39.0 30.0 39.0 12-13 35.301111502953674 38.0 35.0 39.0 29.0 39.0 14-15 36.262627183662545 39.0 36.0 41.0 28.0 41.0 16-17 36.311308927392204 39.0 36.0 41.0 29.0 41.0 18-19 36.27786045570234 39.0 36.0 41.0 28.5 41.0 20-21 36.17507481467459 39.0 36.0 41.0 27.0 41.0 22-23 36.092542111588486 39.0 35.5 41.0 27.0 41.0 24-25 35.91793091544497 39.0 35.0 41.0 27.0 41.0 26-27 35.73073870485274 39.0 35.0 40.0 27.0 41.0 28-29 35.53635925119314 38.5 34.5 40.0 27.0 41.0 30-31 35.31976694756898 38.0 34.0 40.0 26.0 41.0 32-33 35.077076773430775 38.0 34.0 40.0 25.0 41.0 34-35 35.078143866609366 38.0 34.0 40.0 25.0 41.0 36-37 34.959828674330964 38.0 34.0 40.0 25.0 41.0 38-39 34.97463531458123 38.0 34.0 40.0 25.0 41.0 40-41 35.040762728255075 38.0 34.0 40.0 25.0 41.0 42-43 34.9015211311162 38.0 34.0 40.0 24.0 41.0 44-45 34.68798138950455 38.0 34.0 40.0 24.0 41.0 46-47 34.41145900623304 38.0 33.5 40.0 23.0 41.0 48-49 34.156638978837165 38.0 33.0 40.0 21.5 41.0 50-51 33.41629450898431 37.5 32.5 39.5 19.5 40.5 52-53 33.56440252157111 38.0 33.0 39.5 20.5 40.5 54-55 33.8986650611681 38.0 33.0 40.0 20.5 41.0 56-57 33.748711179011465 38.0 33.0 40.0 19.5 41.0 58-59 33.49617405549604 38.0 33.0 40.0 18.0 41.0 60-61 33.25408491590782 37.5 32.5 40.0 17.5 41.0 62-63 32.99729226229661 37.0 32.0 40.0 15.0 41.0 64-65 32.70323610871739 36.5 32.0 40.0 13.0 41.0 66-67 32.37884890068469 36.0 31.0 39.0 13.0 41.0 68-69 32.023785428610836 36.0 31.0 39.0 9.0 41.0 70-71 31.650878165416522 35.0 31.0 39.0 8.0 40.0 72-73 31.30148830538309 35.0 30.5 38.0 7.0 40.0 74-75 30.933280926887743 35.0 30.0 37.5 7.0 40.0 76-77 30.504705407024964 35.0 30.0 37.0 3.5 39.0 78-79 29.96828335542692 34.0 29.0 36.0 2.0 39.0 80-81 29.591134689017274 34.0 29.0 36.0 2.0 38.5 82-83 29.258749868684227 34.0 29.0 35.5 2.0 37.0 84-85 29.022163534309186 34.0 29.0 35.0 2.0 37.0 86-87 28.764385980278362 34.0 29.0 35.0 2.0 36.5 88-89 28.44306648854811 34.0 28.5 35.0 2.0 36.0 90-91 28.104028678081015 34.0 27.0 35.0 2.0 36.0 92-93 27.847527295059418 34.0 27.0 35.0 2.0 35.5 94-95 27.613841594518977 34.0 26.0 35.0 2.0 35.0 96-97 27.324077315646136 34.0 25.5 35.0 2.0 35.0 98-99 27.056719425000004 33.0 25.0 35.0 2.0 35.0 100-101 26.769204916051656 33.0 25.0 35.0 2.0 35.0 102-103 26.487104341395842 33.0 25.0 35.0 2.0 35.0 104-105 26.20161845247987 33.0 24.0 35.0 2.0 35.0 106-107 25.90410044263369 32.5 23.0 35.0 2.0 35.0 108-109 25.58526278694584 32.0 21.0 35.0 2.0 35.0 110-111 25.19264724126426 32.0 19.0 34.5 2.0 35.0 112-113 24.74044669709682 32.0 17.5 34.0 2.0 35.0 114-115 24.247222077386024 31.5 8.5 34.0 2.0 35.0 116-117 23.607488222676622 31.0 2.0 34.0 2.0 35.0 118-119 22.85298771963125 31.0 2.0 34.0 2.0 35.0 120-121 21.198956049220108 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.48800778443780146 1101 2 0.42074033774190767 1101 3 0.48282587276669275 1101 4 0.7397243437832088 1101 5 0.7668875097121699 1101 6 0.747415415616139 1101 7 0.5605145375886593 1101 8 0.5464083762814838 1101 9 0.7029844078255536 1101 10-11 0.7489307743470803 1101 12-13 0.8386806600143046 1101 14-15 1.00209473338397 1101 16-17 0.7844484448195885 1101 18-19 0.753953992395715 1101 20-21 0.8874115181588351 1101 22-23 0.6361112509506199 1101 24-25 0.7394025311715069 1101 26-27 0.8194396586569113 1101 28-29 0.871413665870179 1101 30-31 0.8602989194813588 1101 32-33 0.7018201503453838 1101 34-35 0.7461582378865401 1101 36-37 0.68312918519063 1101 38-39 0.5986825850641964 1101 40-41 0.6332209979229617 1101 42-43 0.5901052399688211 1101 44-45 0.6976987040035638 1101 46-47 0.6453661720561499 1101 48-49 0.6770064708007553 1101 50-51 0.5093168498757663 1101 52-53 0.5175779227397399 1101 54-55 0.6392772949305581 1101 56-57 0.6765614472232429 1101 58-59 0.7396950801816047 1101 60-61 0.675594890328675 1101 62-63 0.534132151073706 1101 64-65 0.6995830939816372 1101 66-67 0.6217689852535173 1101 68-69 0.6352584834742636 1101 70-71 0.3950822404140766 1101 72-73 0.3229980263211658 1101 74-75 0.3402487390934823 1101 76-77 0.3326332785627919 1101 78-79 0.5630261609622913 1101 80-81 0.5250124760201338 1101 82-83 0.5633840577065534 1101 84-85 0.4565633547913066 1101 86-87 0.41622824143137294 1101 88-89 0.521168387695834 1101 90-91 0.45508323458349764 1101 92-93 0.5066163816458946 1101 94-95 0.593138054671595 1101 96-97 0.3906250261593627 1101 98-99 0.449207355853634 1101 100-101 0.4447807192385902 1101 102-103 0.37188888953641097 1101 104-105 0.26042695156026596 1101 106-107 0.14788367318094942 1101 108-109 0.24592239761226864 1101 110-111 0.21592893705296845 1101 112-113 0.19576816476673642 1101 114-115 0.16398499676231637 1101 116-117 0.19226378775318764 1101 118-119 0.291600191608012 1101 120-121 0.26746959386950664 1102 1 0.5386880660228819 1102 2 0.4423303695653722 1102 3 0.5511571225813299 1102 4 0.9269761302020214 1102 5 0.9636629687078937 1102 6 0.7712048253497485 1102 7 0.6154769668486324 1102 8 0.6775267090591157 1102 9 0.7716771653587955 1102 10-11 0.7430285822936824 1102 12-13 0.7062782895661783 1102 14-15 0.8653870733746771 1102 16-17 0.9149773335327325 1102 18-19 0.738989729759119 1102 20-21 0.8183416377507129 1102 22-23 0.7253425448921149 1102 24-25 0.8358861103181141 1102 26-27 0.8071549119010442 1102 28-29 0.8546820820934045 1102 30-31 0.8296701836394718 1102 32-33 0.6246533058831218 1102 34-35 0.6817772855911741 1102 36-37 0.695352145356928 1102 38-39 0.6709114452881053 1102 40-41 0.713750268589564 1102 42-43 0.7011057468435382 1102 44-45 0.7908551314714956 1102 46-47 0.8166435412685473 1102 48-49 0.7571109867488914 1102 50-51 0.33917141098427805 1102 52-53 0.5093166063573449 1102 54-55 0.6331269454656123 1102 56-57 0.6804028880056308 1102 58-59 0.6220075792267181 1102 60-61 0.7308882710302242 1102 62-63 0.5711440033143376 1102 64-65 0.5089515811134007 1102 66-67 0.535175754008101 1102 68-69 0.28115358467396234 1102 70-71 0.21318195902671278 1102 72-73 0.3061827498003389 1102 74-75 0.29422626764787196 1102 76-77 0.200544783989681 1102 78-79 0.41313694993435135 1102 80-81 0.5983668632274792 1102 82-83 0.6167048734513045 1102 84-85 0.5457902675019071 1102 86-87 0.6456872335595385 1102 88-89 0.5174655735525882 1102 90-91 0.5171923501500331 1102 92-93 0.48916638834131376 1102 94-95 0.32950591090610715 1102 96-97 0.6043296736792279 1102 98-99 0.7251904141954526 1102 100-101 0.7074577546685923 1102 102-103 0.7154999539643221 1102 104-105 0.691876977218552 1102 106-107 0.6480586977383069 1102 108-109 0.6733784257815607 1102 110-111 0.6967140940717904 1102 112-113 0.6255607370750234 1102 114-115 0.5664595833763286 1102 116-117 0.6945680905263494 1102 118-119 0.5718683184453219 1102 120-121 0.6106162478223673 1103 1 0.6366099700930974 1103 2 0.6633603904480836 1103 3 0.7125876303850447 1103 4 0.9491522264510692 1103 5 1.0220570815940277 1103 6 0.9294844893966641 1103 7 0.7682654670740732 1103 8 0.8237771093033217 1103 9 0.9936796746449303 1103 10-11 0.9554806580091579 1103 12-13 1.025029742347634 1103 14-15 1.190552468750603 1103 16-17 1.1294075258086522 1103 18-19 0.9961364809897262 1103 20-21 1.110695409754804 1103 22-23 1.1102914723309851 1103 24-25 1.0111202590138504 1103 26-27 1.1509963087743316 1103 28-29 1.2107294103291224 1103 30-31 1.1172102897426939 1103 32-33 1.0823627794651358 1103 34-35 0.9671294678206124 1103 36-37 0.9431729731786405 1103 38-39 0.8315817224764714 1103 40-41 1.0063885290418924 1103 42-43 0.9810996743337483 1103 44-45 0.9888041307909603 1103 46-47 1.0470302915282943 1103 48-49 1.162908786642653 1103 50-51 0.9304571960837151 1103 52-53 1.0141714635716852 1103 54-55 1.0488686551595023 1103 56-57 0.9961630112668445 1103 58-59 0.985656746858794 1103 60-61 0.9827388462149926 1103 62-63 1.154298646473876 1103 64-65 1.031749514499694 1103 66-67 1.0839641226080445 1103 68-69 0.9684231817038871 1103 70-71 0.9542082038445443 1103 72-73 0.8279862571998358 1103 74-75 0.7709653112252468 1103 76-77 0.8409415835296414 1103 78-79 0.9128727654196709 1103 80-81 0.988098417220538 1103 82-83 0.9853413406228775 1103 84-85 0.9927748299778116 1103 86-87 1.0461749713839446 1103 88-89 1.0559620649395214 1103 90-91 1.1287222072010046 1103 92-93 1.0146344056785424 1103 94-95 1.1213202283998847 1103 96-97 0.9976210673318917 1103 98-99 0.9173228033103058 1103 100-101 1.08728215598261 1103 102-103 1.1004165584745031 1103 104-105 1.1502986054353244 1103 106-107 1.0895653989698957 1103 108-109 1.1156888827274223 1103 110-111 1.401041562446661 1103 112-113 1.151998558962383 1103 114-115 1.2055076882958602 1103 116-117 1.0057542276539806 1103 118-119 0.8546318658365699 1103 120-121 0.9621620539632119 1104 1 0.44690100639594377 1104 2 0.5771353263520815 1104 3 0.6715496958532938 1104 4 0.8067594472543718 1104 5 0.9566436096266955 1104 6 0.7247917224842126 1104 7 0.6421502991587431 1104 8 0.718407800001728 1104 9 0.7450161395664239 1104 10-11 0.8710571176253481 1104 12-13 0.9738498490643579 1104 14-15 1.1047935706587637 1104 16-17 0.8794965399434531 1104 18-19 0.8562746460782833 1104 20-21 0.9473443326459048 1104 22-23 0.8554059803471006 1104 24-25 0.9155824516368227 1104 26-27 0.9290357321002674 1104 28-29 0.9784414457860606 1104 30-31 1.036168820286285 1104 32-33 0.8777793935877298 1104 34-35 0.7777263572733872 1104 36-37 0.7288147280863413 1104 38-39 0.7010109527719735 1104 40-41 0.7550320760037081 1104 42-43 0.8438463177870119 1104 44-45 0.9415725898458547 1104 46-47 0.9252805166422746 1104 48-49 1.0330066864568508 1104 50-51 0.6212421381671263 1104 52-53 0.7750913271909567 1104 54-55 0.735820424241858 1104 56-57 0.918783213273862 1104 58-59 0.8893069440943009 1104 60-61 0.7648887995138907 1104 62-63 0.8095864706921958 1104 64-65 0.7669803358725957 1104 66-67 0.6658122262077981 1104 68-69 0.723988735850245 1104 70-71 0.6337799872903744 1104 72-73 0.464969162290199 1104 74-75 0.5809778833427259 1104 76-77 0.624205422707238 1104 78-79 0.7160724491528896 1104 80-81 0.8113551562461332 1104 82-83 0.7346086566838359 1104 84-85 0.6462342077145209 1104 86-87 0.7108121934762792 1104 88-89 0.7705907614882115 1104 90-91 0.8403366607888287 1104 92-93 0.7998955413905122 1104 94-95 0.7544941152249542 1104 96-97 0.6733249753758308 1104 98-99 0.7046562347000176 1104 100-101 0.5693095465788467 1104 102-103 0.5713063431832559 1104 104-105 0.6468870476652384 1104 106-107 0.6423620861406008 1104 108-109 0.733769497890556 1104 110-111 0.7516456872480326 1104 112-113 0.6675303162919626 1104 114-115 0.6746985996351 1104 116-117 0.39865842292075016 1104 118-119 0.33190782468925306 1104 120-121 0.5905112581331409 1105 1 0.6736488985034654 1105 2 0.7522551014774876 1105 3 0.7801153324256553 1105 4 1.001095094476959 1105 5 1.0212528367601905 1105 6 0.973590657609158 1105 7 0.8607647245044632 1105 8 0.9383047807530218 1105 9 1.0615347487390707 1105 10-11 0.9805228931979784 1105 12-13 1.1668119390313834 1105 14-15 1.2917804963376867 1105 16-17 1.2032948062078717 1105 18-19 1.058032865637827 1105 20-21 1.277969824012935 1105 22-23 1.1788403926199607 1105 24-25 1.361993407400476 1105 26-27 1.3656506117802323 1105 28-29 1.2884552329038286 1105 30-31 1.3153022065393714 1105 32-33 1.2917220183633447 1105 34-35 1.332020159599459 1105 36-37 1.486366224824387 1105 38-39 1.2425103736246612 1105 40-41 1.3503552865931567 1105 42-43 1.385506541301794 1105 44-45 1.2909707565052813 1105 46-47 1.513104481387309 1105 48-49 1.397934413928958 1105 50-51 1.2799716394328016 1105 52-53 1.3810912700003115 1105 54-55 1.3869053405655691 1105 56-57 1.480120423789181 1105 58-59 1.5214410779984675 1105 60-61 1.5392603590241904 1105 62-63 1.5904686204123948 1105 64-65 1.5914732045920914 1105 66-67 1.6198621828484576 1105 68-69 1.3330576746299876 1105 70-71 1.152245723099547 1105 72-73 1.0887488293228351 1105 74-75 1.0652252974297376 1105 76-77 1.244294544384136 1105 78-79 1.3975195835211984 1105 80-81 1.434042432898874 1105 82-83 1.4303853915300984 1105 84-85 1.28767685519459 1105 86-87 1.3091466264408673 1105 88-89 1.3845514639071297 1105 90-91 1.5321717514458157 1105 92-93 1.551672470635868 1105 94-95 1.5315636678836668 1105 96-97 1.441261513643365 1105 98-99 1.4671563789038906 1105 100-101 1.4749353202172983 1105 102-103 1.5505294487922825 1105 104-105 1.4940620814703074 1105 106-107 1.378507131184982 1105 108-109 1.4502069969854468 1105 110-111 1.4598219917653843 1105 112-113 1.5457633671820616 1105 114-115 1.4595928079901839 1105 116-117 1.376651592197934 1105 118-119 1.2660084024129326 1105 120-121 1.2360550331525104 1106 1 0.5588630718359511 1106 2 0.7197730090406509 1106 3 0.6887633659936796 1106 4 0.848561085391232 1106 5 0.8450112228398936 1106 6 0.8388414383552032 1106 7 0.7000897456841102 1106 8 0.7785260846706663 1106 9 0.8593424837083816 1106 10-11 0.9814492454201869 1106 12-13 1.0069071632237154 1106 14-15 1.113730939082771 1106 16-17 0.9959153679956714 1106 18-19 0.8907249453855925 1106 20-21 0.9601464803611535 1106 22-23 0.8429353385759981 1106 24-25 1.0391335499123642 1106 26-27 1.030837853585048 1106 28-29 1.0805437145446035 1106 30-31 0.9600448816254996 1106 32-33 1.0036174938417446 1106 34-35 0.9534532138230887 1106 36-37 1.0466561021383924 1106 38-39 1.1117092402172162 1106 40-41 1.0414647944143454 1106 42-43 1.0239698721272532 1106 44-45 0.9828960082592388 1106 46-47 1.0058396440931574 1106 48-49 1.1505956880516024 1106 50-51 0.8886841165219792 1106 52-53 0.949682227694673 1106 54-55 1.0611749702617388 1106 56-57 1.0374853887226791 1106 58-59 1.174764567006882 1106 60-61 1.059487327483673 1106 62-63 1.042754836985047 1106 64-65 0.8566438520342459 1106 66-67 0.8384023904900957 1106 68-69 0.8590264797235854 1106 70-71 0.6523571111992652 1106 72-73 0.7398317986407328 1106 74-75 0.8478416420035657 1106 76-77 1.022324668766995 1106 78-79 0.9799550121111658 1106 80-81 0.80791183276936 1106 82-83 0.976473395303131 1106 84-85 1.0957020386995424 1106 86-87 0.9962512782275077 1106 88-89 0.8730385945421943 1106 90-91 0.770917966246742 1106 92-93 0.7740689648584116 1106 94-95 0.772688834295181 1106 96-97 0.9506277524749258 1106 98-99 0.9938583304258941 1106 100-101 0.7817519537576239 1106 102-103 0.7545291892714232 1106 104-105 0.8946001193678406 1106 106-107 0.9938092858319649 1106 108-109 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-0.7922676687218164 2316 110-111 -0.7404024860796277 2316 112-113 -0.6138496939966025 2316 114-115 -0.5175414219787235 2316 116-117 -0.6009242256885905 2316 118-119 -0.5548371945935173 2316 120-121 -1.038024984045336 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52812.0 3 8472.0 4 7608.0 5 8756.0 6 10321.0 7 11303.0 8 13694.0 9 16521.0 10 20315.0 11 23958.0 12 26865.0 13 31642.0 14 38548.0 15 38205.0 16 28092.0 17 27153.0 18 27972.0 19 30089.0 20 33369.0 21 37256.0 22 41987.0 23 47218.0 24 53698.0 25 61339.0 26 70406.0 27 81841.0 28 95651.0 29 112722.0 30 132073.0 31 159202.0 32 192567.0 33 239246.0 34 306189.0 35 400860.0 36 544236.0 37 622055.0 38 237968.0 39 1078.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.968794147996405 20.237886660651036 14.68930518939821 22.10401400195435 2 14.307870122676194 20.537931581250536 41.55932380800135 23.59487448807192 3 19.057657061042892 26.126913209455658 30.31137310622254 24.50405662327891 4 11.806785619737347 16.764617048029017 40.46462880829166 30.963968523941976 5 11.838311374409793 38.22622086658173 36.18279489109828 13.752672867910196 6 28.652789573473957 38.470640418644585 18.292045949756638 14.584524058124815 7 26.10734197517689 33.10776367172323 23.248405168528382 17.5364891845715 8 24.020822471569048 35.38574382268317 20.78691102690195 19.806522678845827 9 24.1281089018904 18.327078622875923 22.981308387009886 34.56350408822379 10-11 23.67250532414448 26.82181431512798 28.72357658202549 20.782103778702048 12-13 25.12945977614734 24.462773168188356 28.869682230327363 21.538084825336938 14-15 21.724216695982946 26.371287036403977 27.86227357577792 24.042222691835157 16-17 20.142459422896216 29.84693933720556 28.509836098484676 21.500765141413545 18-19 19.883547890835963 29.44916061249233 30.50459125675046 20.162700239921246 20-21 21.05767110620085 27.819084339921684 29.88269949726505 21.24054505661242 22-23 20.592082666112685 27.62253331267993 30.179570640599167 21.605813380608215 24-25 21.118954511100394 27.825022650429155 30.10001155184837 20.95601128662208 26-27 20.7043929436898 27.407993829302935 30.99170696026863 20.89590626673864 28-29 20.155345429975903 28.463380272202954 30.63184827433979 20.74942602348135 30-31 20.637864829666807 28.018540135943205 30.068816244266124 21.274778790123865 32-33 20.544612715341835 28.097359490792527 30.337273394862873 21.020754399002765 34-35 21.211128753425395 27.548966059043252 30.361405168875642 20.87850001865571 36-37 21.14217517738987 27.534002208854385 30.67816027617417 20.64566233758157 38-39 20.944019525541773 28.141355491299525 30.58067499922271 20.33394998393599 40-41 20.46097756264916 28.317315291550816 30.44405691349739 20.777650232302634 42-43 20.211399585585507 28.555747092633844 29.669165400716846 21.563687921063806 44-45 20.62237999650526 28.26796194022127 29.47915272861184 21.630505334661628 46-47 21.1067504941313 28.425424383015873 29.379790742194277 21.08803438065855 48-49 21.184247118570436 28.3202419415112 29.337143936313677 21.158367003604695 50-51 20.561999470031857 28.64590547778442 29.69119961334353 21.100895438840187 52-53 20.62624247391282 29.07452751502886 28.985809680101543 21.313420330956774 54-55 20.383538580504613 28.79293572655866 29.192414057294748 21.631111635641975 56-57 21.206776125047845 28.315250195615693 28.922828588029915 21.55514509130655 58-59 21.487690361018995 27.933786277396322 29.09965641794131 21.478866943643368 60-61 21.030919579134626 28.29171035778948 29.833996194401696 20.843373868674202 62-63 20.66824990951109 28.567186817041918 29.44924964808429 21.315313625362702 64-65 20.373040231774482 29.177511989116983 29.201165090095515 21.248282689013017 66-67 20.542234543850395 28.205038525166394 29.044183892269178 22.208543038714033 68-69 20.48038735298515 28.73252334215809 29.202009525761557 21.5850797790952 70-71 21.048353753635546 28.365780237113995 29.02824100521762 21.557625004032836 72-73 20.72805238517032 27.99484140414564 29.67313430437737 21.603971906306676 74-75 20.324140699568993 29.027624549937762 29.337332677142395 21.31090207335085 76-77 20.749726846489867 28.3283160693576 29.17691599805452 21.745041086098013 78-79 20.64057580896959 28.570876576057923 29.20636958201725 21.582178032955234 80-81 20.90238497035859 28.453081203337334 28.87038389538976 21.774149930914316 82-83 20.570653075873057 28.227234348874163 29.81389588459097 21.38821669066181 84-85 20.71229036160412 28.515435757290312 29.231266911757274 21.541006969348295 86-87 20.86253160410462 28.673215890631916 28.58253711894961 21.881715386313854 88-89 20.820877787807003 27.934085965745663 28.591544404716274 22.65349184173106 90-91 20.590241333947954 27.05476891240647 29.630360156150477 22.724629597495102 92-93 21.07119635343146 27.592814358843054 29.55201048214824 21.783978805577245 94-95 20.46821192859562 27.53740043674765 29.580894324638717 22.413493310018012 96-97 19.572865243005086 27.773736990489873 30.1629372424484 22.490460524056637 98-99 20.029790831600007 28.420754080586907 29.50014349686964 22.049311590943443 100-101 19.931426251790395 27.889496051384427 29.332411724000902 22.846665972824276 102-103 20.153546436783248 27.777379694423153 29.353958226298943 22.715115642494656 104-105 20.571322108420652 27.543977255118605 29.53881861154025 22.34588202492049 106-107 20.309831187389086 27.99985955280005 29.801522452830014 21.888786806980846 108-109 20.494332934280695 27.368105329166603 29.408391506916047 22.729170229636654 110-111 20.221456954328794 27.6221070865448 28.984391903534785 23.172044055591616 112-113 20.784590656099553 26.93290902482684 28.63676855210553 23.645731766968076 114-115 20.763938731170956 26.990181536551365 28.775666516659303 23.470213215618372 116-117 21.32635367513041 26.697335202202616 28.35717938993533 23.619131732731645 118-119 21.178099221862 26.835081350790958 28.58575576384358 23.401063663503464 120-121 21.442986084331604 27.862500362819127 27.83758246360406 22.85693108924521 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9508.0 1 10046.5 2 6278.0 3 1954.0 4 1919.0 5 1933.0 6 2008.5 7 2231.0 8 2615.5 9 2827.0 10 2686.0 11 2622.5 12 2854.5 13 3145.5 14 3514.0 15 3914.5 16 4317.5 17 4780.0 18 5269.5 19 5883.0 20 6644.5 21 7739.5 22 9298.5 23 11562.5 24 14601.5 25 18379.5 26 23424.0 27 30062.5 28 38475.0 29 48520.5 30 59168.0 31 69494.0 32 80125.0 33 89975.0 34 98522.0 35 108046.0 36 117995.5 37 129621.0 38 139252.0 39 142637.5 40 145670.0 41 149063.5 42 152421.5 43 157364.5 44 158195.5 45 158594.0 46 161704.5 47 174385.0 48 211710.0 49 205017.0 50 158630.0 51 132591.0 52 110789.5 53 94231.5 54 81119.5 55 69091.5 56 57572.5 57 46147.5 58 37018.5 59 30192.5 60 24083.5 61 18360.5 62 13378.5 63 9426.0 64 6338.5 65 4116.0 66 2578.0 67 1539.5 68 897.5 69 559.5 70 376.0 71 247.5 72 179.5 73 140.5 74 95.5 75 67.5 76 44.5 77 34.5 78 33.0 79 28.0 80 23.0 81 21.0 82 16.5 83 9.5 84 10.0 85 10.5 86 12.0 87 9.5 88 6.5 89 5.0 90 3.5 91 5.0 92 4.5 93 3.0 94 3.5 95 3.5 96 2.5 97 1.5 98 1.0 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4579164084230112 2 0.8633835625269857 3 0.8648989915205326 4 0.8432206513416555 5 0.8527755595721559 6 0.8628441725462316 7 0.8396760886109861 8 0.8287598628100112 9 0.8269875814446764 10-11 0.8185499810314523 12-13 0.8207075409544686 14-15 0.8206690130987003 16-17 0.8447746081909707 18-19 0.87596932874458 20-21 0.8681096461678781 22-23 0.8410630914186393 24-25 0.8330492974188648 26-27 0.8337171135855127 28-29 0.8571934203669033 30-31 0.8645779260557981 32-33 0.858053875812392 34-35 0.8701901503793581 36-37 0.8803101338277913 38-39 0.8662859943281859 40-41 0.8757895320843287 42-43 0.8698562422960342 44-45 0.8527884021907453 46-47 0.8470220664441126 48-49 0.8522490122099913 50-51 0.8877973804654011 52-53 0.8552284997227279 54-55 0.8669666531134231 56-57 0.9142788599967071 58-59 0.8794111505265345 60-61 0.8928445295710283 62-63 0.8655411224500018 64-65 0.8560375846938589 66-67 0.8580795610495706 68-69 0.8750446602061446 70-71 0.8820695725745367 72-73 0.8857554041096893 74-75 0.8900962091929004 76-77 0.9017701494906488 78-79 0.95412950547956 80-81 0.983526259430656 82-83 0.9490951989925223 84-85 0.9091032847051862 86-87 0.8889917439942135 88-89 0.8877845378468117 90-91 0.9011793890355373 92-93 0.8870653512058063 94-95 0.8812091171290479 96-97 0.8916758512793945 98-99 0.9262096526662431 100-101 0.9129175424262327 102-103 0.8866415447923567 104-105 0.8767142006227642 106-107 0.8780626755746495 108-109 0.8770737939432669 110-111 0.8751474011548596 112-113 0.8851004305616308 114-115 0.8883881409205127 116-117 0.9168602263331729 118-119 0.9115176969999899 120-121 0.8890944849429286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 3893287.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.60002258925336 #Duplication Level Percentage of deduplicated Percentage of total 1 77.67770762165523 43.18882298445449 2 13.531143935727687 15.04663816969796 3 3.933623891717228 6.561287317113136 4 1.659516549718584 3.690766306063722 5 0.8333365605389883 2.3166765795209225 6 0.4859682228157255 1.6211906499728188 7 0.33313231544493077 1.2965514984763897 8 0.24064641670723946 1.0703956963956311 9 0.1775473487149264 0.8884472939290757 >10 0.9893044001354857 10.266219241907741 >50 0.08280774646484762 3.1712445895405397 >100 0.050315875884699476 4.990506995372538 >500 0.002567936773358399 0.9601906060629347 >1k 0.0018209005949519391 2.2100374747010254 >5k 4.668975884492149E-4 1.7833149430552024 >10k+ 9.337951768984298E-5 0.9377096537358801 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21001 0.5394156659912305 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15121 0.3883864713800961 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9542 0.2450885331597696 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9157 0.23519971684594537 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8530 0.21909507313486007 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7250 0.1862179695460417 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 6278 0.16125191900828273 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6132 0.15750187438018312 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5987 0.1537775149892623 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5709 0.14663701905356577 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5095 0.13086628342580447 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5016 0.1288371496886821 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4783 0.12285248942602998 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4305 0.11057494605458063 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4154 0.10669647524058719 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3910 0.10042927736896869 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 2.568523717876437E-5 0.0 0.0 0.0 5 2.568523717876437E-5 2.568523717876437E-5 0.0 0.0 0.0 6 2.568523717876437E-5 2.568523717876437E-5 0.0 0.0 0.0 7 2.568523717876437E-5 2.568523717876437E-5 0.0 0.0 0.0 8 2.568523717876437E-5 2.568523717876437E-5 0.0 0.0 0.0 9 1.0274094871505748E-4 2.568523717876437E-5 0.0 0.0 0.0 10-11 1.1558356730443967E-4 2.568523717876437E-5 0.0 7.705571153629312E-5 0.0 12-13 1.2842618589382185E-4 2.568523717876437E-5 0.0 1.0274094871505748E-4 0.0 14-15 1.2842618589382185E-4 7.705571153629312E-5 0.0 2.0548189743011496E-4 0.0 16-17 1.2842618589382185E-4 8.989833012567529E-5 0.0 2.4400975319826152E-4 0.0 18-19 1.2842618589382185E-4 1.0274094871505748E-4 0.0 3.0822284614517246E-4 0.0 20-21 1.2842618589382185E-4 1.0274094871505748E-4 0.0 4.494916506283765E-4 0.0 22-23 1.2842618589382185E-4 1.0274094871505748E-4 0.0 9.503537756142817E-4 0.0 24-25 1.2842618589382185E-4 1.0274094871505748E-4 0.0 0.0018493370768710347 0.0 26-27 1.2842618589382185E-4 1.0274094871505748E-4 0.0 0.0034931922563119543 0.0 28-29 1.2842618589382185E-4 1.0274094871505748E-4 0.0 0.009940186788181812 0.0 30-31 1.2842618589382185E-4 1.2842618589382185E-4 0.0 0.02685391547039815 0.0 32-33 1.2842618589382185E-4 1.2842618589382185E-4 0.0 0.0563790956073878 0.0 34-35 1.2842618589382185E-4 1.5411142307258623E-4 0.0 0.0790848452734155 0.0 36-37 1.2842618589382185E-4 1.797966602513506E-4 0.0 0.09113122151025599 0.0 38-39 1.2842618589382185E-4 1.797966602513506E-4 0.0 0.10220155873430342 0.0 40-41 1.2842618589382185E-4 1.797966602513506E-4 0.0 0.11840894339410375 0.0 42-43 1.2842618589382185E-4 1.797966602513506E-4 0.0 0.13611891442886176 0.0 44-45 1.2842618589382185E-4 1.797966602513506E-4 0.0 0.15336655119440207 0.0 46-47 1.2842618589382185E-4 1.797966602513506E-4 0.0 0.1750320487546898 0.0 48-49 1.4126880448320404E-4 1.797966602513506E-4 0.0 0.20020358118987888 0.0 50-51 1.5411142307258623E-4 1.797966602513506E-4 0.0 0.23424936307033106 0.0 52-53 1.5411142307258623E-4 1.9263927884073277E-4 0.0 0.3202178519076554 0.0 54-55 1.5411142307258623E-4 2.0548189743011496E-4 0.0 0.4040287808219636 0.0 56-57 1.5411142307258623E-4 2.0548189743011496E-4 0.0 0.5006694857070645 0.0 58-59 1.5411142307258623E-4 2.0548189743011496E-4 0.0 0.6771527503623544 2.568523717876437E-5 60-61 1.5411142307258623E-4 2.3116713460887933E-4 0.0 0.9054046105514441 2.568523717876437E-5 62-63 1.5411142307258623E-4 2.3116713460887933E-4 0.0 1.0121010857920312 2.568523717876437E-5 64-65 1.5411142307258623E-4 2.3116713460887933E-4 0.0 1.1317043927149477 2.568523717876437E-5 66-67 1.5411142307258623E-4 2.3116713460887933E-4 0.0 1.5207715228802807 2.568523717876437E-5 68-69 1.5411142307258623E-4 2.3116713460887933E-4 0.0 1.8906260956358985 2.568523717876437E-5 70-71 1.5411142307258623E-4 2.3116713460887933E-4 0.0 2.0844468953868542 2.568523717876437E-5 72-73 1.5411142307258623E-4 2.3116713460887933E-4 0.0 2.2213106816939003 2.568523717876437E-5 74-75 1.797966602513506E-4 2.3116713460887933E-4 0.0 2.7017530431226877 2.568523717876437E-5 76-77 1.797966602513506E-4 2.3116713460887933E-4 0.0 2.8525510705992136 2.568523717876437E-5 78-79 1.797966602513506E-4 2.3116713460887933E-4 0.0 2.9459811208369686 2.568523717876437E-5 80-81 1.797966602513506E-4 2.3116713460887933E-4 0.0 3.1141166834091605 2.568523717876437E-5 82-83 1.797966602513506E-4 3.0822284614517246E-4 0.0 3.5226660659745868 2.568523717876437E-5 84-85 1.797966602513506E-4 3.0822284614517246E-4 0.0 3.911335074963649 2.568523717876437E-5 86-87 1.797966602513506E-4 3.595933205027012E-4 0.0 4.64633097945258 2.568523717876437E-5 88-89 1.797966602513506E-4 3.595933205027012E-4 0.0 5.635546518918334 2.568523717876437E-5 90-91 1.797966602513506E-4 3.7243593909208335E-4 0.0 6.720272612833321 2.568523717876437E-5 92-93 1.797966602513506E-4 3.981211762708477E-4 0.0 7.712827233132312 2.568523717876437E-5 94-95 1.797966602513506E-4 4.366490320389943E-4 0.0 8.577251047765039 2.568523717876437E-5 96-97 1.797966602513506E-4 4.6233426921775866E-4 0.0 9.53945856033732 2.568523717876437E-5 98-99 1.797966602513506E-4 4.6233426921775866E-4 0.0 10.521546446485964 2.568523717876437E-5 100-101 1.797966602513506E-4 4.6233426921775866E-4 0.0 11.284449361169624 2.568523717876437E-5 102-103 1.797966602513506E-4 4.8801950639652304E-4 0.0 12.143389377664683 2.568523717876437E-5 104-105 1.797966602513506E-4 4.8801950639652304E-4 0.0 13.094526553012917 2.568523717876437E-5 106-107 1.797966602513506E-4 4.8801950639652304E-4 0.0 14.010834546746747 2.568523717876437E-5 108-109 1.797966602513506E-4 5.008621249859052E-4 0.0 14.963320197046865 2.568523717876437E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3350 0.0 28.32945 2 TAACGCA 615 0.0 26.155535 4 GTATAAC 1360 0.0 25.435066 1 TATAACG 665 0.0 25.082762 2 ACCTGGG 3725 0.0 24.830847 3 GAGTACT 11515 0.0 24.526524 12-13 ATAACGC 890 0.0 23.238306 3 GTACATG 14540 0.0 23.037334 1 AGTACTT 12505 0.0 22.5848 12-13 GTACTTT 13140 0.0 21.7566 14-15 TACATGG 14155 0.0 21.414097 2 ACGCGAT 215 4.663034E-6 21.366035 8 GTACACG 550 0.0 20.96466 1 ACATGGG 14165 0.0 20.643969 3 GTACCTG 5105 0.0 20.102255 1 CATGGGG 6365 0.0 19.585833 4 GTACTAG 1210 0.0 18.582314 1 CCGGTCG 495 1.8189894E-12 18.559671 9 GTCGCCG 1290 0.0 18.047237 114-115 TAACGCC 455 1.8007995E-10 17.676542 4 >>END_MODULE