FastQCFastQC Report
Wed 25 May 2016
SRR1003866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3893287
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT261370.6713350441413644No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT126170.32407063748447007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123350.3168274006000585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105330.2705426032039251No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC81320.20887234873771185No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT76680.1969543986867652No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA69130.1775620446167981No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT67940.17450550139252513No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64240.1650019636363823No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC62660.16094369616213755No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC58360.14989904417526886No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT57900.14871752326504573No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA54230.13929104122043917No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG52030.13364028904111103No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA45010.11560925254161844No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA44040.11311778453527829No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC39220.10073750021511386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG30800.030.084652
TAACGCA6200.028.3420474
TATAACG6100.028.04362
ACCTGGG32700.027.3162423
ATAACGC7250.024.2479233
GTACCTG39350.024.1649741
TATCACG5500.022.4632032
GTATAAC12550.021.9727971
TCACGCA6000.021.3714334
AGTACTT146100.020.07305512-13
GAGTACT142250.019.8651312-13
GTACTTT153550.018.32825314-15
GTACATG147300.018.139891
GTACTAG9900.017.2889421
TACATGG146150.016.8744492
CATGGGG67550.016.522044
ACATGGG143250.016.4843793
TACTTTT183300.015.97545114-15
CACGCAG8050.015.9292245
CTAACGC1800.002615402715.8376073
Produced by FastQC (version 0.11.5)