##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1003866_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3893287
Sequences flagged as poor quality	0
Sequence length	101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.588968139261247	31.0	31.0	34.0	30.0	34.0
2	31.828223298205348	31.0	31.0	34.0	30.0	34.0
3	31.84909075544649	31.0	31.0	34.0	30.0	34.0
4	35.631716593202604	37.0	35.0	37.0	33.0	37.0
5	35.48476595740309	37.0	35.0	37.0	33.0	37.0
6	35.50651570254132	37.0	35.0	37.0	33.0	37.0
7	35.42732683205733	37.0	35.0	37.0	33.0	37.0
8	35.421388405221606	37.0	35.0	37.0	33.0	37.0
9	36.977555982900824	39.0	37.0	39.0	33.0	39.0
10-11	36.90610633123117	39.0	37.0	39.0	33.0	39.0
12-13	36.844065310366275	39.0	37.0	39.0	32.0	39.0
14-15	37.954492951585635	40.0	37.0	41.0	33.0	41.0
16-17	37.955527809791576	40.0	37.0	41.0	33.0	41.0
18-19	37.96769747516687	40.0	37.0	41.0	33.0	41.0
20-21	37.91569951046506	40.0	37.0	41.0	32.5	41.0
22-23	37.83241936697705	40.0	37.0	41.0	32.0	41.0
24-25	37.74492183597047	39.5	37.0	41.0	32.0	41.0
26-27	37.5337112316662	39.0	36.5	41.0	32.0	41.0
28-29	37.35458007591015	39.0	36.0	41.0	31.5	41.0
30-31	37.13628907912517	39.0	36.0	40.0	31.0	41.0
32-33	36.89933904179168	39.0	35.5	40.0	31.0	41.0
34-35	36.89597414729508	39.0	35.0	40.0	30.5	41.0
36-37	36.7245217216198	39.0	35.0	40.0	30.0	41.0
38-39	36.47892295122348	38.5	35.0	40.0	30.0	41.0
40-41	36.58855075415709	39.0	35.0	40.0	30.0	41.0
42-43	36.449241733270625	39.0	35.0	40.5	30.0	41.0
44-45	36.29368718514715	39.0	35.0	41.0	30.0	41.0
46-47	36.02798278164441	39.0	35.0	40.0	29.5	41.0
48-49	35.7819996316737	39.0	35.0	40.0	28.0	41.0
50-51	35.552885518072515	39.0	35.0	40.0	27.5	41.0
52-53	35.35373220623087	38.0	34.5	40.0	27.0	41.0
54-55	35.14988196349255	38.0	34.0	40.0	26.5	41.0
56-57	34.92543126155354	38.0	34.0	40.0	26.0	41.0
58-59	34.665067332565	38.0	34.0	40.0	26.0	41.0
60-61	34.27217900966458	37.5	33.0	40.0	25.0	41.0
62-63	34.04561274316535	37.0	33.0	40.0	25.0	41.0
64-65	33.61327369392495	36.5	32.5	39.5	23.0	41.0
66-67	33.2874366826797	36.0	32.0	39.0	22.5	41.0
68-69	33.04604785108316	36.0	32.0	39.0	22.0	40.5
70-71	32.660362053966224	35.0	31.5	39.0	22.0	40.0
72-73	32.30165680053898	35.0	31.0	38.0	21.5	40.0
74-75	31.924010225806626	35.0	31.0	37.0	20.0	39.5
76-77	30.924288268499087	34.0	30.0	36.0	20.0	39.0
78-79	31.31591506097547	34.5	31.0	36.0	20.0	39.0
80-81	31.143616820439902	35.0	31.0	36.0	19.5	38.5
82-83	30.832793857735123	34.0	31.0	36.0	18.5	37.0
84-85	30.478508648347784	34.0	30.5	35.0	17.5	37.0
86-87	30.11410384079057	34.0	30.0	35.0	13.0	36.5
88-89	29.799188962950844	34.0	30.0	35.0	8.5	36.0
90-91	29.502816514682838	34.0	29.5	35.0	7.0	36.0
92-93	29.25082841311211	34.0	29.0	35.0	2.0	35.5
94-95	28.924523288419273	34.0	29.0	35.0	2.0	35.0
96-97	28.54570482474064	34.0	29.0	35.0	2.0	35.0
98-99	28.140094860717948	34.0	29.0	35.0	2.0	35.0
100-101	27.178317062163668	33.0	27.0	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.056167606781961865
1101	2	0.04701434078019062
1101	3	0.09937148716884536
1101	4	0.0811263018468722
1101	5	0.17323314179193972
1101	6	0.1785722913432295
1101	7	0.14404291731454322
1101	8	0.14465280858517815
1101	9	0.21420279739564307
1101	10-11	0.16647064396180866
1101	12-13	0.11331392075882007
1101	14-15	0.14003982874483967
1101	16-17	0.14472580986944195
1101	18-19	0.08300537367828298
1101	20-21	0.09230112293566606
1101	22-23	0.09193214574127495
1101	24-25	0.05713250097111455
1101	26-27	0.15682581478665725
1101	28-29	0.13358045975832766
1101	30-31	0.03546870288617043
1101	32-33	0.08546170191349489
1101	34-35	0.04599348877629694
1101	36-37	0.012432175929184552
1101	38-39	-0.022963529801195648
1101	40-41	0.0132513936384413
1101	42-43	0.003946548749432566
1101	44-45	0.043601035415129274
1101	46-47	0.030160625449255463
1101	48-49	0.030422269301439542
1101	50-51	-0.08964336957763663
1101	52-53	-0.06148282708013397
1101	54-55	0.04738433348430249
1101	56-57	0.021183857417703678
1101	58-59	-0.22627934657656112
1101	60-61	-0.06547921221341824
1101	62-63	-0.19419905250740044
1101	64-65	-0.37590022756110386
1101	66-67	-0.27136795669019875
1101	68-69	-0.3889589422525148
1101	70-71	-0.2543739824696516
1101	72-73	-0.35241534220654813
1101	74-75	-0.4012434155868192
1101	76-77	-0.3041503605745355
1101	78-79	-0.2905795923088945
1101	80-81	-0.3078813015621371
1101	82-83	-0.46036202125329595
1101	84-85	-0.6116141200162524
1101	86-87	-0.5382602758900745
1101	88-89	-0.49758422704291405
1101	90-91	-0.48195632677148836
1101	92-93	-0.3606933863730397
1101	94-95	-0.4929548940999098
1101	96-97	-0.4237517580746655
1101	98-99	-0.5161612502533188
1101	100-101	-0.4463354278653675
1102	1	-0.03713598510840299
1102	2	0.0353108373232871
1102	3	0.025816686732788696
1102	4	0.015120923942120612
1102	5	-0.04372676028417999
1102	6	0.022621591200071123
1102	7	-0.04513076905762148
1102	8	-0.010900659956682546
1102	9	-0.07649704020673198
1102	10-11	0.013862710653583576
1102	12-13	0.017623456743784516
1102	14-15	0.026333069247009178
1102	16-17	-0.027554524200539277
1102	18-19	-0.029288607364243546
1102	20-21	-0.08405353118974546
1102	22-23	-0.21045344571590618
1102	24-25	-0.11682056458738543
1102	26-27	-0.11017538319914877
1102	28-29	0.021609678748880867
1102	30-31	-0.003436627476609999
1102	32-33	-0.061274707957622354
1102	34-35	-0.06829799653737467
1102	36-37	-0.050532290715487704
1102	38-39	0.030194394025599536
1102	40-41	0.015201603083745852
1102	42-43	0.13178929356588043
1102	44-45	0.17020029703168404
1102	46-47	0.21690373972703725
1102	48-49	0.26011481473817355
1102	50-51	0.29290111002649866
1102	52-53	0.29595405398674757
1102	54-55	0.2648731287671282
1102	56-57	0.29214962000801137
1102	58-59	0.06310803152411637
1102	60-61	0.1098868564788944
1102	62-63	0.03482103015632276
1102	64-65	0.030221340587033296
1102	66-67	0.02118446754596448
1102	68-69	-0.014625451045084503
1102	70-71	-0.009708973630800699
1102	72-73	0.06472917819920809
1102	74-75	0.06255436117440993
1102	76-77	0.08437876809525235
1102	78-79	0.14123216574650144
1102	80-81	0.09905270406920863
1102	82-83	-0.00385157954433879
1102	84-85	-0.0463935441382759
1102	86-87	-0.04206756553931612
1102	88-89	0.06521790938007754
1102	90-91	0.0244583569575596
1102	92-93	0.062323893201618574
1102	94-95	0.01056750320179134
1102	96-97	0.002101301282699808
1102	98-99	-0.08878093874105986
1102	100-101	-0.1607141109078043
1103	1	-0.05373970180178489
1103	2	-0.07860309826983425
1103	3	-0.06915204520954532
1103	4	-0.05023067017338434
1103	5	-0.022784303032132414
1103	6	-0.018018948893896436
1103	7	-0.009633658750786367
1103	8	0.013794524323685664
1103	9	-0.06516767866509099
1103	10-11	0.03809113784087259
1103	12-13	0.03666982999449431
1103	14-15	0.06552502870261634
1103	16-17	-0.024029924701466143
1103	18-19	0.032371988348494085
1103	20-21	0.09771439998411324
1103	22-23	0.05061628219271341
1103	24-25	-0.043278200313345394
1103	26-27	-0.07030954319040461
1103	28-29	-0.009544956942541205
1103	30-31	0.12082437935577417
1103	32-33	0.12642582255849533
1103	34-35	0.035040740287243466
1103	36-37	-0.04357422603976602
1103	38-39	0.006817331776076685
1103	40-41	0.17364976644126529
1103	42-43	0.25925517128859354
1103	44-45	0.18625020643413137
1103	46-47	0.1978250435476312
1103	48-49	0.16885796857851432
1103	50-51	0.2120922713274851
1103	52-53	0.2171549546412308
1103	54-55	0.2315817957928914
1103	56-57	0.24341037759212014
1103	58-59	0.22945337482862982
1103	60-61	0.22215885730785345
1103	62-63	0.08331662536963336
1103	64-65	0.04389709003556419
1103	66-67	0.027897251369282117
1103	68-69	0.06299742042588008
1103	70-71	-0.020569944429539078
1103	72-73	0.1405166440909298
1103	74-75	0.12749789290128888
1103	76-77	0.03619298290246675
1103	78-79	0.13153377017733447
1103	80-81	0.1324522079997692
1103	82-83	0.02701436688501957
1103	84-85	0.0050861458603357335
1103	86-87	0.1316621083561742
1103	88-89	0.07212999848213997
1103	90-91	0.11489161022649341
1103	92-93	0.13566986942874593
1103	94-95	-0.08128690872627686
1103	96-97	0.034524699442368245
1103	98-99	0.043313185627301465
1103	100-101	-0.04762960266907257
1104	1	-0.08881500047552748
1104	2	-0.07452750379527373
1104	3	-0.06453886946260212
1104	4	-0.08146341366884968
1104	5	-6.354884148578321E-4
1104	6	-0.07598734272809793
1104	7	0.019937099065728603
1104	8	-0.06962631772263705
1104	9	-0.1179891033154874
1104	10-11	0.03194362647212756
1104	12-13	-0.11195152025116073
1104	14-15	0.01027982637508984
1104	16-17	-0.01585090972983494
1104	18-19	-0.07009356624073604
1104	20-21	-0.12316419604124462
1104	22-23	-0.05162549872867572
1104	24-25	0.01960362807602678
1104	26-27	-0.04225954306220103
1104	28-29	-0.1255481774185796
1104	30-31	-0.07951464940590114
1104	32-33	-0.03826262272500003
1104	34-35	0.014797381761503914
1104	36-37	0.12724200473056158
1104	38-39	0.01097590898579881
1104	40-41	0.020325323060781386
1104	42-43	0.13081191224889466
1104	44-45	0.15022089767798263
1104	46-47	0.14286337527764204
1104	48-49	0.12648199571666652
1104	50-51	0.29207355475804064
1104	52-53	0.3098143566667133
1104	54-55	0.13596479351622293
1104	56-57	0.16240835989954405
1104	58-59	0.14789320500306502
1104	60-61	0.15390564504851056
1104	62-63	0.15831731300746554
1104	64-65	-0.02419024416505522
1104	66-67	0.016290624032386347
1104	68-69	0.022837098247741494
1104	70-71	0.05774810042263567
1104	72-73	0.11265734691273366
1104	74-75	0.09057276104382339
1104	76-77	0.058178104750727044
1104	78-79	0.12160582413289589
1104	80-81	0.1200159572716295
1104	82-83	0.3254481668252964
1104	84-85	0.2578583461312398
1104	86-87	0.17244972523420898
1104	88-89	0.1854727889724117
1104	90-91	0.10225827550234712
1104	92-93	0.054794278031071286
1104	94-95	-0.02696241266574262
1104	96-97	0.11627528084598282
1104	98-99	0.0038168127420519227
1104	100-101	0.11624944043742858
1105	1	0.04097517613556079
1105	2	0.08278959721346624
1105	3	0.0597740757745342
1105	4	0.1589896761354268
1105	5	0.05680312430212808
1105	6	0.11870365966188245
1105	7	0.05793672254020521
1105	8	0.17655270911842535
1105	9	0.13282531108301043
1105	10-11	0.13836986048298883
1105	12-13	0.11326428238270125
1105	14-15	0.13965530945807103
1105	16-17	0.05931882743620065
1105	18-19	0.012745577342876402
1105	20-21	0.03582701991774684
1105	22-23	0.10538794573614751
1105	24-25	0.06393343350756453
1105	26-27	0.1411393754210053
1105	28-29	0.144511471440687
1105	30-31	0.22789638048467964
1105	32-33	0.17842974904952769
1105	34-35	0.17671710229807758
1105	36-37	0.23897079933588827
1105	38-39	0.3006285690993522
1105	40-41	0.3747605617752612
1105	42-43	0.5268316214209747
1105	44-45	0.5175385129116066
1105	46-47	0.2503735622075922
1105	48-49	0.3797564975505736
1105	50-51	0.4414409415604581
1105	52-53	0.3312827239818432
1105	54-55	0.33690752481862773
1105	56-57	0.44085815841911113
1105	58-59	0.25748759816001865
1105	60-61	0.33292730091154255
1105	62-63	0.2366235765120024
1105	64-65	0.1384310128852846
1105	66-67	0.18130707677413938
1105	68-69	0.23533068270936042
1105	70-71	0.30099810109762615
1105	72-73	0.3154053573287996
1105	74-75	0.20841405111746525
1105	76-77	0.21372349239897304
1105	78-79	0.29196285206524664
1105	80-81	0.3193876947356493
1105	82-83	0.40619767352531966
1105	84-85	0.3170104077066611
1105	86-87	0.27569853423292656
1105	88-89	0.3730289517796912
1105	90-91	0.5121960825185319
1105	92-93	0.5012683460015381
1105	94-95	0.46543650900835587
1105	96-97	0.5373023045295398
1105	98-99	0.48738646861392
1105	100-101	0.48268870066049985
1106	1	-0.06723410497918181
1106	2	-0.04757808645056727
1106	3	-0.04676410311269663
1106	4	-0.006605905521936961
1106	5	0.04457888081167738
1106	6	-0.011457093547505792
1106	7	0.011501204116100894
1106	8	0.010099211388606477
1106	9	0.014173309840302295
1106	10-11	-0.05209433873920233
1106	12-13	-0.0812814438202949
1106	14-15	-0.0029616963948697617
1106	16-17	0.022721021136078434
1106	18-19	0.09096444700475814
1106	20-21	-0.04281116932536122
1106	22-23	-0.011583689132983466
1106	24-25	-0.04903056920242932
1106	26-27	0.028016416184492243
1106	28-29	-0.004278481358959141
1106	30-31	0.029781369074491693
1106	32-33	0.16345679910835287
1106	34-35	0.08377095412414093
1106	36-37	-0.009072750260671114
1106	38-39	0.05042965509432662
1106	40-41	-0.19953841997967459
1106	42-43	-0.09458911180031748
1106	44-45	-0.014774485465139264
1106	46-47	-0.12741220082740057
1106	48-49	-0.18720184935146023
1106	50-51	0.0925594078420815
1106	52-53	-0.00489763428171841
1106	54-55	-0.10063737665702632
1106	56-57	0.0939304549416633
1106	58-59	-0.01591806413596686
1106	60-61	0.10922434374620593
1106	62-63	-0.1247845487367556
1106	64-65	0.0190679806335865
1106	66-67	-0.024058111497012646
1106	68-69	-0.08672650046619879
1106	70-71	0.05832522963655862
1106	72-73	0.09699952659379818
1106	74-75	0.007097513025918545
1106	76-77	-0.15096442129037868
1106	78-79	-0.12026029969081975
1106	80-81	-0.11126820680612681
1106	82-83	-0.04207220820553914
1106	84-85	-0.04052624025703366
1106	86-87	-0.03010132028567014
1106	88-89	0.03778258787904676
1106	90-91	0.04580509438038405
1106	92-93	0.04776041626680794
1106	94-95	0.18717899194922794
1106	96-97	0.18690591137040968
1106	98-99	0.08545734276252404
1106	100-101	0.1784796902958874
1107	1	-0.10406535937310224
1107	2	-0.09912202532825987
1107	3	-0.09144968473904314
1107	4	-0.15563222997386816
1107	5	-0.07565744321777146
1107	6	0.048957385341779514
1107	7	-0.10400584302195881
1107	8	-0.005765002596611168
1107	9	-0.10493999481472116
1107	10-11	0.002122196587372116
1107	12-13	-0.0539379828063673
1107	14-15	-0.0364946816696019
1107	16-17	-0.15013800661995447
1107	18-19	-0.041455366743257116
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2316	92-93	-0.3913989981174488
2316	94-95	-0.6033325562988097
2316	96-97	-0.4296345775822772
2316	98-99	-0.4674202214510075
2316	100-101	-0.9749260356497942
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	22.0
4	30.0
5	69.0
6	208.0
7	565.0
8	1297.0
9	2475.0
10	4300.0
11	6937.0
12	9899.0
13	13971.0
14	17357.0
15	22447.0
16	28396.0
17	34996.0
18	31386.0
19	21934.0
20	19461.0
21	19940.0
22	21558.0
23	23811.0
24	28902.0
25	36144.0
26	45495.0
27	57665.0
28	72757.0
29	90699.0
30	112408.0
31	138137.0
32	171336.0
33	211857.0
34	273082.0
35	357927.0
36	496503.0
37	688810.0
38	678123.0
39	152295.0
40	84.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.67465014873947	21.414371501230416	15.421363386860353	21.489614963169764
2	15.105916960199039	21.582483046273957	40.367476073676045	22.944123919850956
3	19.261446272284836	26.60969868899133	30.632120997209416	23.49673404151442
4	12.70658957328345	17.77274575442293	39.230912080203694	30.289752592089926
5	12.264983290468953	38.83291933011874	35.45698018152785	13.445117197884462
6	28.849423320787654	39.314360099649676	17.63680575479697	14.199410824765705
7	26.33815590784856	33.406681808970156	22.744893967488142	17.51026831569314
8	24.507158090323163	36.019769413351746	20.018046447641797	19.455026048683287
9	24.269338479284986	18.510014802402186	22.567229181922627	34.6534175363902
10-11	24.071126209505028	27.393083948699765	28.22978280633292	20.30600703546229
12-13	25.416954876432175	24.8277612207885	28.67659127107763	21.078692631701696
14-15	21.901895116614508	26.877788281873183	27.5571025871968	23.66321401431551
16-17	20.287561641358575	30.23744460657537	28.336403147263482	21.138590604802573
18-19	20.12200744512285	29.641110454995996	30.502580467353162	19.73430163252799
20-21	21.230428917819243	28.029897269238546	30.050205546294862	20.68946826664735
22-23	20.691217986241444	27.941775163248945	30.287826199301517	21.079180651208095
24-25	21.221952042066253	28.073309262841402	30.380729702177106	20.32400899291524
26-27	20.93231979891998	27.625024804278986	31.143616935880058	20.29903846092098
28-29	20.46252626603577	28.68009638158064	30.744645325030955	20.11273202735264
30-31	20.880983542968995	28.24173198916479	30.244138956783758	20.633145511082454
32-33	20.850317109692945	28.286241121525812	30.413568680728382	20.44987308805286
34-35	21.483593545304625	27.722117760687187	30.49552014138269	20.298768552625496
36-37	21.377549552363963	27.742541454632146	30.769235709764136	20.11067328323975
38-39	21.083592396964086	28.3450419305468	30.706977841004544	19.864387831484567
40-41	20.71703414314352	28.434727333049302	30.439688615497012	20.408549908310167
42-43	20.487906491352938	28.68210075445247	29.787696103575207	21.042296650619384
44-45	20.963121907015843	28.375419022538022	29.55040303989919	21.11105603054694
46-47	21.39505309846915	28.716388767276378	29.276224412517397	20.612333721737077
48-49	21.484545064363353	28.56947098942359	29.291290367239814	20.654693578973244
50-51	20.849541788211347	28.923015436570694	29.51020564371443	20.717237131503534
52-53	20.963391602006222	29.353076205273332	28.74758526663973	20.93594692608071
54-55	20.74867586181034	29.032819825509907	28.920498283327174	21.298006029352575
56-57	21.601903811540126	28.640021781330088	28.686679547960342	21.071394859169445
58-59	21.895571100001657	28.2758203525279	28.821529812711926	21.007078734758515
60-61	21.302462946120716	28.73815609708857	29.5380526260966	20.421328330694113
62-63	21.04512959788895	28.838368969670064	29.204275949377497	20.912225483063494
64-65	20.70042355105772	29.48344501898688	28.90234555933088	20.913785870624523
66-67	20.926771963561645	28.44716946351508	28.824039087152865	21.802019485770405
68-69	20.843991330019097	29.079895258493423	28.83780670596849	21.23830670551899
70-71	21.39390263276081	28.714110668652488	28.70707288383892	21.18491381474778
72-73	21.05221115217039	28.318988556456283	29.27572511350948	21.353075177863843
74-75	20.650581706707833	29.38351546316286	28.940110985966864	21.025791844162452
76-77	21.067134274971252	28.661064545203065	28.687635923064498	21.58416525676119
78-79	20.79820984170959	28.938529319826667	28.752581045271004	21.510679793192743
80-81	21.213638198380263	28.897251434553155	28.396697339560635	21.492413027505947
82-83	20.875689359659333	28.717045519634183	29.227180015241622	21.18008510546487
84-85	20.70776505230097	28.924351386175708	28.766772314423033	21.601111247100295
86-87	20.80040942578291	28.894454065779108	28.096959936505613	22.208176571932373
88-89	20.998195098383448	28.67046534201049	28.094640852318363	22.236698707287697
90-91	21.02661581332175	27.637096879834445	28.931350296035202	22.404937010808602
92-93	20.69218118263565	28.337237917471793	28.936012166583147	22.034568733309413
94-95	20.375184693122065	28.389386077172666	28.939269112888677	22.296160116816594
96-97	20.030503885610944	28.82445098592457	29.364985695203355	21.78005943326113
98-99	20.084748973386716	29.076539277790797	28.52533061924286	22.313381129579625
100-101	19.366966438600265	29.356968332910593	28.277571044663986	22.99849418382516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	367.0
1	273.5
2	358.5
3	533.5
4	430.0
5	350.0
6	562.5
7	1161.5
8	1801.5
9	2261.5
10	2376.0
11	2315.0
12	2645.0
13	3145.5
14	3565.5
15	4022.5
16	4528.0
17	5111.5
18	5685.0
19	6372.0
20	7225.0
21	8230.5
22	9793.5
23	12092.5
24	14912.0
25	18597.5
26	23666.0
27	30110.0
28	38952.0
29	49528.0
30	59696.5
31	69898.0
32	80675.5
33	90228.0
34	98763.0
35	108402.5
36	117863.0
37	128977.0
38	139258.5
39	144062.5
40	146837.0
41	150134.0
42	153979.5
43	159252.5
44	160490.0
45	160336.5
46	163818.5
47	178092.5
48	219448.5
49	211437.0
50	161038.0
51	134296.5
52	110448.0
53	93191.5
54	80424.0
55	68697.0
56	57051.0
57	45060.0
58	35751.0
59	29070.5
60	23087.5
61	17366.0
62	12506.0
63	8709.0
64	5658.0
65	3476.5
66	2087.0
67	1197.0
68	653.5
69	353.5
70	230.0
71	163.0
72	107.0
73	78.5
74	51.0
75	33.0
76	19.0
77	14.5
78	10.0
79	6.0
80	4.5
81	3.0
82	2.0
83	1.5
84	1.0
85	0.5
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04928997014604883
2	0.004880195063965231
3	0.04178988088984963
4	0.0
5	0.0
6	0.0012328913845806898
7	0.0
8	0.0
9	0.0
10-11	2.696949903770259E-4
12-13	0.0
14-15	0.002478625387750762
16-17	0.0
18-19	0.0
20-21	0.012585766217594541
22-23	0.0
24-25	0.0
26-27	0.007949580906827573
28-29	0.010967596275332387
30-31	0.010839170089438565
32-33	0.00599750288124148
34-35	0.007885367813880662
36-37	0.021549913992983307
38-39	0.010813484852259801
40-41	0.013959926406658434
42-43	0.0
44-45	0.0
46-47	3.852785576814656E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0011429930544550146
58-59	1.6695404166196842E-4
60-61	0.010492419387525246
62-63	0.016207384659800317
64-65	0.04583530574550502
66-67	0.024644985073024414
68-69	0.002144717304426825
70-71	4.2380641344961215E-4
72-73	0.0
74-75	5.137047435752874E-5
76-77	0.0
78-79	0.0
80-81	3.852785576814656E-5
82-83	0.0
84-85	8.989833012567529E-5
86-87	7.57714496773549E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.1558356730443968E-4
96-97	3.210654647345546E-4
98-99	6.292883108797271E-4
100-101	0.0012714192403488363
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3893287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.64455740305345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.51778263850967	38.484379030227736
2	15.212714505088115	15.713078150285742
3	4.585402680377086	7.104332758285488
4	1.9361964370637772	3.9997603215011126
5	0.9711522003520654	2.5077362779091956
6	0.5898763464593517	1.8278341701254066
7	0.3804007345897284	1.3751939301578051
8	0.27809224317256076	1.1489560652695372
9	0.20357083212884963	0.9461992972919161
>10	1.1565583410568334	11.108496597827088
>50	0.10112349539062299	3.624352653538849
>100	0.06097054233796991	5.743488504726829
>500	0.003576195586671403	1.2804219215803558
>1k	0.001887436532576211	1.9018559392238275
>5k	4.966938243621604E-4	1.653215632529962
>10k+	1.9867752974486418E-4	1.580698749519175
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	26137	0.6713350441413644	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	12617	0.32407063748447007	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12335	0.3168274006000585	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10533	0.2705426032039251	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	8132	0.20887234873771185	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	7668	0.1969543986867652	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	6913	0.1775620446167981	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	6794	0.17450550139252513	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6424	0.1650019636363823	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	6266	0.16094369616213755	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	5836	0.14989904417526886	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	5790	0.14871752326504573	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	5423	0.13929104122043917	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	5203	0.13364028904111103	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	4501	0.11560925254161844	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	4404	0.11311778453527829	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	3922	0.10073750021511386	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	2.568523717876437E-5	0.0	0.0	0.0
5	0.0	2.568523717876437E-5	0.0	0.0	0.0
6	0.0	2.568523717876437E-5	0.0	0.0	0.0
7	0.0	2.568523717876437E-5	0.0	0.0	0.0
8	0.0	2.568523717876437E-5	0.0	0.0	0.0
9	5.137047435752874E-5	2.568523717876437E-5	0.0	1.0274094871505748E-4	0.0
10-11	6.421309294691093E-5	2.568523717876437E-5	0.0	1.797966602513506E-4	0.0
12-13	7.705571153629312E-5	2.568523717876437E-5	0.0	1.9263927884073277E-4	0.0
14-15	7.705571153629312E-5	6.421309294691093E-5	0.0	2.696949903770259E-4	0.0
16-17	7.705571153629312E-5	1.0274094871505748E-4	0.0	2.9538022755579027E-4	0.0
18-19	8.989833012567529E-5	1.0274094871505748E-4	0.0	3.5959332050270116E-4	0.0
20-21	1.0274094871505748E-4	1.0274094871505748E-4	0.0	4.8801950639652304E-4	0.0
22-23	1.0274094871505748E-4	1.0274094871505748E-4	0.0	9.760390127930461E-4	0.0
24-25	1.0274094871505748E-4	1.0274094871505748E-4	0.0	0.0019392354069967099	0.0
26-27	1.0274094871505748E-4	1.0274094871505748E-4	0.0	0.0036473036793845406	0.0
28-29	1.0274094871505748E-4	1.0274094871505748E-4	0.0	0.010299780108684512	0.0
30-31	1.0274094871505748E-4	1.0274094871505748E-4	0.0	0.02786848233895934	0.0
32-33	1.0274094871505748E-4	1.0274094871505748E-4	0.0	0.05867792433488721	0.0
34-35	1.0274094871505748E-4	1.0274094871505748E-4	0.0	0.08193590660025835	0.0
36-37	1.0274094871505748E-4	1.2842618589382185E-4	0.0	0.09466294162233609	0.0
38-39	1.2842618589382185E-4	1.5411142307258623E-4	0.0	0.10599013121817118	0.0
40-41	1.2842618589382185E-4	1.6695404166196842E-4	0.0	0.12308365656063885	0.0
42-43	1.2842618589382185E-4	1.9263927884073277E-4	0.0	0.1415898699479386	0.0
44-45	1.2842618589382185E-4	2.1832451601949714E-4	0.0	0.15928699836410726	0.0
46-47	1.2842618589382185E-4	2.3116713460887933E-4	0.0	0.18156894161668535	0.0
48-49	1.2842618589382185E-4	2.3116713460887933E-4	0.0	0.20761377211595242	0.0
50-51	1.2842618589382185E-4	2.3116713460887933E-4	0.0	0.24237874063740997	0.0
52-53	1.2842618589382185E-4	2.3116713460887933E-4	0.0	0.329747074900977	0.0
54-55	1.4126880448320404E-4	2.3116713460887933E-4	0.0	0.41503490495306405	0.0
56-57	1.5411142307258623E-4	2.3116713460887933E-4	0.0	0.513357992873374	0.0
58-59	1.5411142307258623E-4	2.3116713460887933E-4	0.0	0.6929876990830628	0.0
60-61	1.5411142307258623E-4	3.0822284614517246E-4	0.0	0.92606838386176	0.0
62-63	1.797966602513506E-4	3.3390808332393684E-4	0.0	1.0340234357241065	0.0
64-65	1.797966602513506E-4	3.3390808332393684E-4	0.0	1.1552705978264637	0.0
66-67	1.797966602513506E-4	3.3390808332393684E-4	0.0	1.5526212169819487	0.0
68-69	1.797966602513506E-4	3.3390808332393684E-4	0.0	1.9314527801315444	0.0
70-71	1.797966602513506E-4	3.3390808332393684E-4	0.0	2.1319645841675685	0.0
72-73	2.0548189743011496E-4	3.3390808332393684E-4	0.0	2.2738755195802414	0.0
74-75	2.568523717876437E-4	3.3390808332393684E-4	0.0	2.7685475024060646	0.0
76-77	2.568523717876437E-4	3.3390808332393684E-4	0.0	2.9255099868054937	0.0
78-79	2.568523717876437E-4	3.3390808332393684E-4	0.0	3.024051399241823	0.0
80-81	2.568523717876437E-4	3.46750701913319E-4	0.0	3.1985825858715273	0.0
82-83	2.568523717876437E-4	3.8527855768146553E-4	0.0	3.6219138224333323	0.0
84-85	2.825376089664081E-4	3.8527855768146553E-4	0.0	4.023091541928452	0.0
86-87	2.825376089664081E-4	3.8527855768146553E-4	0.0	4.782604005304515	0.0
88-89	2.825376089664081E-4	3.8527855768146553E-4	0.0	5.801447465855972	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	3080	0.0	30.08465	2
TAACGCA	620	0.0	28.342047	4
TATAACG	610	0.0	28.0436	2
ACCTGGG	3270	0.0	27.316242	3
ATAACGC	725	0.0	24.247923	3
GTACCTG	3935	0.0	24.164974	1
TATCACG	550	0.0	22.463203	2
GTATAAC	1255	0.0	21.972797	1
TCACGCA	600	0.0	21.371433	4
AGTACTT	14610	0.0	20.073055	12-13
GAGTACT	14225	0.0	19.86513	12-13
GTACTTT	15355	0.0	18.328253	14-15
GTACATG	14730	0.0	18.13989	1
GTACTAG	990	0.0	17.288942	1
TACATGG	14615	0.0	16.874449	2
CATGGGG	6755	0.0	16.52204	4
ACATGGG	14325	0.0	16.484379	3
TACTTTT	18330	0.0	15.975451	14-15
CACGCAG	805	0.0	15.929224	5
CTAACGC	180	0.0026154027	15.837607	3
>>END_MODULE