##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003865_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2613997 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.752023051288887 31.0 31.0 34.0 27.0 34.0 2 30.670536729766713 31.0 31.0 34.0 27.0 34.0 3 30.73353718462569 31.0 31.0 34.0 27.0 34.0 4 34.25668124332201 37.0 35.0 37.0 30.0 37.0 5 34.233859870535426 37.0 35.0 37.0 30.0 37.0 6 34.34526015140798 37.0 35.0 37.0 30.0 37.0 7 34.360344330923105 37.0 35.0 37.0 30.0 37.0 8 34.337165268361055 37.0 35.0 37.0 30.0 37.0 9 35.78163632169432 39.0 35.0 39.0 30.0 39.0 10-11 35.620942946759314 39.0 35.0 39.0 30.0 39.0 12-13 35.487936099391085 38.0 35.0 39.0 30.0 39.0 14-15 36.44305464007801 39.0 36.0 41.0 29.5 41.0 16-17 36.49354647308317 39.0 36.0 41.0 30.0 41.0 18-19 36.46233469280952 39.0 36.0 41.0 29.5 41.0 20-21 36.335120315746344 39.0 36.0 41.0 29.0 41.0 22-23 36.21219955493446 39.0 35.5 41.0 29.0 41.0 24-25 36.02803101916337 39.0 35.0 41.0 27.0 41.0 26-27 35.833788256069155 39.0 35.0 40.5 27.0 41.0 28-29 35.63208947829703 38.5 35.0 40.0 27.0 41.0 30-31 35.35847038079998 38.0 34.5 40.0 26.5 41.0 32-33 35.02396808412557 38.0 34.0 40.0 25.0 41.0 34-35 34.967280375608695 38.0 34.0 40.0 25.0 41.0 36-37 34.78003417754496 38.0 34.0 40.0 24.5 41.0 38-39 34.752233648317116 38.0 34.0 40.0 24.5 41.0 40-41 34.79845998293035 38.0 34.0 40.0 24.0 41.0 42-43 34.627008179427904 38.0 34.0 40.0 23.5 41.0 44-45 34.36004785009317 38.0 33.5 40.0 22.5 41.0 46-47 34.010967686649984 38.0 33.0 40.0 19.5 41.0 48-49 33.69484414098409 38.0 33.0 40.0 16.0 41.0 50-51 32.90346603305207 37.5 32.0 39.5 14.5 40.5 52-53 33.021866321958285 37.5 32.5 39.5 14.0 40.5 54-55 33.312407588838084 38.0 33.0 40.0 13.5 41.0 56-57 33.122211693433464 38.0 32.5 40.0 10.5 41.0 58-59 32.865032744873076 38.0 32.0 40.0 8.0 41.0 60-61 32.554231699577315 37.0 31.5 40.0 8.0 41.0 62-63 32.241476749973316 36.5 31.0 40.0 7.0 41.0 64-65 31.925234611975455 36.0 31.0 39.0 4.0 41.0 66-67 31.567933895869047 36.0 31.0 39.0 2.0 41.0 68-69 31.163373370359643 35.0 30.0 39.0 2.0 40.5 70-71 30.73042528357913 35.0 30.0 38.0 2.0 40.0 72-73 30.348655717661497 35.0 29.0 37.5 2.0 40.0 74-75 29.989853278331996 35.0 29.0 37.0 2.0 39.0 76-77 29.56938607810185 34.0 29.0 36.5 2.0 39.0 78-79 29.018899600879422 34.0 28.5 36.0 2.0 39.0 80-81 28.618179936702298 34.0 27.5 35.5 2.0 37.5 82-83 28.282183950478903 34.0 27.0 35.0 2.0 37.0 84-85 28.04160161622221 34.0 26.0 35.0 2.0 37.0 86-87 27.79566254284148 34.0 26.0 35.0 2.0 36.0 88-89 27.4748002771235 34.0 26.0 35.0 2.0 36.0 90-91 27.139316533263045 33.5 25.0 35.0 2.0 35.5 92-93 26.866214077521896 33.0 25.0 35.0 2.0 35.0 94-95 26.614639764314955 33.0 25.0 35.0 2.0 35.0 96-97 26.309735818365517 33.0 24.0 35.0 2.0 35.0 98-99 26.058334611707664 33.0 23.5 35.0 2.0 35.0 100-101 25.74170781374271 32.5 21.5 35.0 2.0 35.0 102-103 25.415125189508633 32.0 19.5 35.0 2.0 35.0 104-105 25.092231934466643 32.0 18.5 35.0 2.0 35.0 106-107 24.733057076959156 32.0 17.5 35.0 2.0 35.0 108-109 24.382344547449748 32.0 10.5 34.0 2.0 35.0 110-111 23.980984102124065 31.5 4.5 34.0 2.0 35.0 112-113 23.48566869051495 31.0 2.0 34.0 2.0 35.0 114-115 22.95894237828123 31.0 2.0 34.0 2.0 35.0 116-117 22.29781881922588 31.0 2.0 34.0 2.0 35.0 118-119 21.52043154601937 30.0 2.0 34.0 2.0 35.0 120-121 19.91835109221625 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.453435384774032 1101 2 0.3128326886321453 1101 3 0.35667748189924353 1101 4 0.6878308729316416 1101 5 0.6214208481872419 1101 6 0.753747456118731 1101 7 0.6148444871311156 1101 8 0.6941028396976137 1101 9 0.6840787131261052 1101 10-11 0.7091156143360351 1101 12-13 0.8582944558505332 1101 14-15 1.0061137550013868 1101 16-17 0.7142210001143425 1101 18-19 0.6765388325738826 1101 20-21 0.8561620530189487 1101 22-23 0.6435997295979163 1101 24-25 0.65421290748084 1101 26-27 0.6616777333687764 1101 28-29 0.6154171513767821 1101 30-31 0.6183727109497283 1101 32-33 0.5074378995366047 1101 34-35 0.5346946154590597 1101 36-37 0.4689832824872724 1101 38-39 0.4702047382463945 1101 40-41 0.6705950033203507 1101 42-43 0.5163889163819704 1101 44-45 0.7523768938113591 1101 46-47 0.6488104000260577 1101 48-49 0.8512159645869346 1101 50-51 0.27265368545497637 1101 52-53 0.3070991682272606 1101 54-55 0.546738476196289 1101 56-57 0.5016763311185102 1101 58-59 0.5806686188741779 1101 60-61 0.3891980408676048 1101 62-63 0.2912501345694558 1101 64-65 0.40173922109135773 1101 66-67 0.22508780862259314 1101 68-69 0.25128602084089735 1101 70-71 0.09862685912806768 1101 72-73 0.07253729591185376 1101 74-75 0.14001507402858593 1101 76-77 0.010473888053731173 1101 78-79 0.3413577870261051 1101 80-81 0.305306589397329 1101 82-83 0.41033371339460345 1101 84-85 0.3468943736275669 1101 86-87 0.39233041933220036 1101 88-89 0.4702931065406446 1101 90-91 0.5079112499000225 1101 92-93 0.45289138709735255 1101 94-95 0.4664602643174547 1101 96-97 0.4986005442034305 1101 98-99 0.5563282366705558 1101 100-101 0.5156229577773281 1101 102-103 0.2257068190601963 1101 104-105 0.13670963479860987 1101 106-107 0.17448904416953326 1101 108-109 0.20034368979957762 1101 110-111 0.22531373475874616 1101 112-113 0.3408670605310782 1101 114-115 0.24763930382956545 1101 116-117 0.43162854502228853 1101 118-119 0.3748711928776238 1101 120-121 0.3130416817997812 1102 1 0.4932031365444871 1102 2 0.464625540122622 1102 3 0.619493964925752 1102 4 0.797421217462805 1102 5 0.973044386530745 1102 6 0.7540135770199683 1102 7 0.7023791832712547 1102 8 0.7453516998967729 1102 9 0.7988511345652043 1102 10-11 0.7964365350540916 1102 12-13 0.9569609139721393 1102 14-15 1.0015177717198114 1102 16-17 0.9649613685461773 1102 18-19 0.8060310035982425 1102 20-21 0.8906053783050609 1102 22-23 0.7580897114012757 1102 24-25 0.9340505837733843 1102 26-27 0.8882589685312823 1102 28-29 0.9167712046491587 1102 30-31 0.8302108481837536 1102 32-33 0.5862191009569884 1102 34-35 0.788910999870204 1102 36-37 0.6469379525576073 1102 38-39 0.5206764500967154 1102 40-41 0.5895556656300087 1102 42-43 0.7431722527949418 1102 44-45 0.7450480246142988 1102 46-47 0.711036872929931 1102 48-49 0.6775591470715554 1102 50-51 0.378809312958019 1102 52-53 0.46805498914496724 1102 54-55 0.49300477484620586 1102 56-57 0.5821240519185196 1102 58-59 0.6149083365650867 1102 60-61 0.8170075997295285 1102 62-63 0.6703079761983979 1102 64-65 0.6673947873363879 1102 66-67 0.5301045390913295 1102 68-69 0.5212450884335986 1102 70-71 0.354942460440796 1102 72-73 0.3640313187359858 1102 74-75 0.47485793214697836 1102 76-77 0.519334458777621 1102 78-79 0.7170979044004859 1102 80-81 0.8453393323104841 1102 82-83 0.7941936564748566 1102 84-85 0.654421050992628 1102 86-87 0.6554059936512324 1102 88-89 0.6380205746362613 1102 90-91 0.6737929435938561 1102 92-93 0.7522510399551869 1102 94-95 0.462321833245781 1102 96-97 0.6007932297951442 1102 98-99 0.6720205326211008 1102 100-101 0.5953993509453532 1102 102-103 0.5298622969330005 1102 104-105 0.5666260678144717 1102 106-107 0.5152687834313028 1102 108-109 0.7033121931356305 1102 110-111 0.6365496102663819 1102 112-113 0.21217613937115232 1102 114-115 0.2575818066514515 1102 116-117 0.379481903367644 1102 118-119 0.061860667544920034 1102 120-121 0.17862526987274308 1103 1 0.5587225962113465 1103 2 0.751541979495709 1103 3 0.6939805733718991 1103 4 1.0098651239095986 1103 5 0.8927023618245542 1103 6 0.8473394868198696 1103 7 0.7852412473471944 1103 8 0.7809742340742218 1103 9 0.8002440897153846 1103 10-11 0.8106853923304058 1103 12-13 0.893452950355794 1103 14-15 1.0892257244927848 1103 16-17 1.0183539858838841 1103 18-19 0.9270997630340503 1103 20-21 1.0436995946218204 1103 22-23 1.0192171918206583 1103 24-25 1.0326814006122973 1103 26-27 1.0949905598365746 1103 28-29 1.1988117796810727 1103 30-31 1.0679857378733857 1103 32-33 0.9620654567527609 1103 34-35 0.9131188608916432 1103 36-37 0.9996186092483796 1103 38-39 0.8943087787507125 1103 40-41 1.0103084035792804 1103 42-43 0.8387359095941491 1103 44-45 0.9488469552918417 1103 46-47 1.0436442743595862 1103 48-49 1.047459899017106 1103 50-51 1.019934645557342 1103 52-53 0.8942030823812033 1103 54-55 0.7271205506888592 1103 56-57 0.75758609389068 1103 58-59 0.7030554976891281 1103 60-61 0.6953463651708987 1103 62-63 0.7165210357785092 1103 64-65 0.6565256187721253 1103 66-67 0.6824777760021412 1103 68-69 0.6088917866294317 1103 70-71 0.5606629602364421 1103 72-73 0.4245367654408554 1103 74-75 0.4484585126839775 1103 76-77 0.45468142555522206 1103 78-79 0.746579341396373 1103 80-81 0.6712056023602777 1103 82-83 0.6310721962074446 1103 84-85 0.5903312992222389 1103 86-87 0.5866058952268567 1103 88-89 0.6884046799336652 1103 90-91 0.7686452653247926 1103 92-93 0.5468980676224469 1103 94-95 0.5400426833654741 1103 96-97 0.5164188293080265 1103 98-99 0.49465647086397624 1103 100-101 0.6642554907913762 1103 102-103 0.609927763739794 1103 104-105 0.7058947350816958 1103 106-107 0.6865331925931137 1103 108-109 0.6527737784353675 1103 110-111 0.7250674267466302 1103 112-113 0.6997252994275378 1103 114-115 0.8250111058365306 1103 116-117 0.523806028183099 1103 118-119 0.3533383573246347 1103 120-121 0.5993795717686616 1104 1 0.4140449224639049 1104 2 0.5639266232757016 1104 3 0.5746896575693548 1104 4 0.6922957423867331 1104 5 0.7563806731772829 1104 6 0.6501516617250687 1104 7 0.4596514624550849 1104 8 0.6287375818375693 1104 9 0.6452472777418308 1104 10-11 0.7713174605206206 1104 12-13 0.7871154893671672 1104 14-15 0.8464659003376127 1104 16-17 0.8225462406575375 1104 18-19 0.6768187133343915 1104 20-21 0.7530438679195797 1104 22-23 0.6286126413323885 1104 24-25 0.7874969984511466 1104 26-27 0.7696189030695706 1104 28-29 0.6701321260014126 1104 30-31 0.8077337159934501 1104 32-33 0.8398507851194879 1104 34-35 0.6572419858287262 1104 36-37 0.624929022349491 1104 38-39 0.4518442665187621 1104 40-41 0.5618831208516681 1104 42-43 0.5979266542500099 1104 44-45 0.6512446867593411 1104 46-47 0.7598511940548818 1104 48-49 0.7050910275784688 1104 50-51 0.4698910199323265 1104 52-53 0.7464003037644744 1104 54-55 0.5960549109022892 1104 56-57 0.6988131484898261 1104 58-59 0.5731601991891822 1104 60-61 0.720712149257615 1104 62-63 0.8290625640177076 1104 64-65 0.6528551935900744 1104 66-67 0.6620198747302801 1104 68-69 0.6586627520980706 1104 70-71 0.3992523424304757 1104 72-73 0.316768887324141 1104 74-75 0.5511842752934619 1104 76-77 0.4945569247330468 1104 78-79 0.5266097115430206 1104 80-81 0.6357807558889199 1104 82-83 0.5640942774853386 1104 84-85 0.5855366752415705 1104 86-87 0.5999117108582546 1104 88-89 0.6031836993638464 1104 90-91 0.6994399228636361 1104 92-93 0.6762564353529079 1104 94-95 0.6756722346927013 1104 96-97 0.5127903518423409 1104 98-99 0.6584790823610085 1104 100-101 0.4413159626192886 1104 102-103 0.5186663120132273 1104 104-105 0.3799358102855628 1104 106-107 0.3605071514507934 1104 108-109 0.5813535965500698 1104 110-111 0.3920990385456804 1104 112-113 0.6263797173610257 1104 114-115 0.5733835344415183 1104 116-117 0.5299094209143966 1104 118-119 0.2553359075779973 1104 120-121 0.5251866458315497 1105 1 0.6706187619578579 1105 2 0.8601045131399729 1105 3 0.7575112933682924 1105 4 0.9829081744136019 1105 5 1.038029982905897 1105 6 1.0469221014042986 1105 7 0.9865778189162384 1105 8 1.0138601478537055 1105 9 0.9438820939600063 1105 10-11 1.010342502585054 1105 12-13 1.1514906203985475 1105 14-15 1.2853538890190634 1105 16-17 1.266217722623395 1105 18-19 0.9916885574176106 1105 20-21 1.1964846450639683 1105 22-23 1.2104888545325636 1105 24-25 1.3607605942926568 1105 26-27 1.272712331546721 1105 28-29 1.3245311837786105 1105 30-31 1.3942155781787804 1105 32-33 1.261419657260248 1105 34-35 1.20077416175981 1105 36-37 1.3830020912061443 1105 38-39 1.3095311335844002 1105 40-41 1.2619676848880275 1105 42-43 1.3208568832243799 1105 44-45 1.3774527160806755 1105 46-47 1.4063405642262339 1105 48-49 1.5066571069429742 1105 50-51 1.2822790432994893 1105 52-53 1.3593338016437855 1105 54-55 1.2660220324254325 1105 56-57 1.1998400249295713 1105 58-59 1.3826018564036673 1105 60-61 1.413055014635809 1105 62-63 1.314329133359582 1105 64-65 1.269550619964587 1105 66-67 1.1507747905668602 1105 68-69 1.0349710500225306 1105 70-71 0.90767054058038 1105 72-73 0.9314744286171681 1105 74-75 0.9258375781033656 1105 76-77 1.004829656869454 1105 78-79 1.0171943309997395 1105 80-81 1.079309591239781 1105 82-83 0.9387260137141489 1105 84-85 0.9243858056795808 1105 86-87 0.9475153373204606 1105 88-89 0.9235299191453556 1105 90-91 1.0505674134837726 1105 92-93 1.0577883256067828 1105 94-95 1.0589316272907432 1105 96-97 1.078434672971163 1105 98-99 0.9497900284312415 1105 100-101 1.0441790190530789 1105 102-103 1.1426826647293495 1105 104-105 1.292534968561739 1105 106-107 1.2781025890117625 1105 108-109 1.2295006496591512 1105 110-111 1.1814941426629915 1105 112-113 1.175552994152799 1105 114-115 1.174380729634052 1105 116-117 1.283722994011793 1105 118-119 1.2937311414787693 1105 120-121 1.1856663275523331 1106 1 0.4908527783415302 1106 2 0.6979461758999079 1106 3 0.6719358013271268 1106 4 0.966643330687809 1106 5 0.9361581552803457 1106 6 0.9299220694024513 1106 7 0.8062915183974653 1106 8 0.788910742010728 1106 9 0.794618334089634 1106 10-11 1.0113649680099854 1106 12-13 0.930980737883587 1106 14-15 0.9588485941156577 1106 16-17 0.8071736497035502 1106 18-19 0.8295752905095739 1106 20-21 0.9540092549314778 1106 22-23 0.8074908550943221 1106 24-25 1.0617949297258278 1106 26-27 1.0765142913603043 1106 28-29 1.1367285925978834 1106 30-31 1.0000574199450654 1106 32-33 1.0057113753986826 1106 34-35 1.0497603725331572 1106 36-37 1.0325200171497855 1106 38-39 0.9846036190455578 1106 40-41 0.9003624186332857 1106 42-43 0.839179535037772 1106 44-45 0.7478017542466446 1106 46-47 0.9101861409014518 1106 48-49 1.0474306489878558 1106 50-51 0.7528136284363214 1106 52-53 0.6351209482990754 1106 54-55 0.709351157919464 1106 56-57 0.5953605566651419 1106 58-59 0.8812174258510552 1106 60-61 0.8291311239556549 1106 62-63 1.0102352044926768 1106 64-65 0.6949942822407955 1106 66-67 0.7395787081030711 1106 68-69 0.6661194688571115 1106 70-71 0.43140220597568657 1106 72-73 0.5516330175371067 1106 74-75 0.6865976258230937 1106 76-77 0.8037610246348237 1106 78-79 0.8101152799323117 1106 80-81 0.6947080008626756 1106 82-83 0.9146756048108529 1106 84-85 0.8904512243421685 1106 86-87 0.8668991755201354 1106 88-89 0.8865151280441133 1106 90-91 0.7915870382665666 1106 92-93 0.7578395285639097 1106 94-95 0.6741784425012334 1106 96-97 0.8895565774457772 1106 98-99 0.9121128883203937 1106 100-101 0.7438204453563309 1106 102-103 0.6952500990621324 1106 104-105 0.6442649234518818 1106 106-107 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2205 18-19 1.7911919749036542 2205 20-21 1.8758345704507988 2205 22-23 1.0728967709137507 2205 24-25 1.7238696441782082 2205 26-27 1.8170181111198076 2205 28-29 2.1151178202979324 2205 30-31 -4.186156551417078 2205 32-33 -1.7711743201637944 2205 34-35 1.2912164410050764 2205 36-37 2.0265489593179495 2205 38-39 2.011715493331792 2205 40-41 2.134590383564216 2205 42-43 2.0716465111298348 2205 44-45 2.109857225364827 2205 46-47 2.292649040938443 2205 48-49 2.1143178056406953 2205 50-51 2.101821248119343 2205 52-53 1.8727635467273345 2205 54-55 1.8172498390435052 2205 56-57 1.9328325776313093 2205 58-59 1.9767352408726637 2205 60-61 1.8248623331603326 2205 62-63 1.8754034754155988 2205 64-65 1.754978270722372 2205 66-67 1.9158500683854562 2205 68-69 1.9341664143678763 2205 70-71 1.9529324852592431 2205 72-73 1.8979413090556 2205 74-75 1.8033372945958384 2205 76-77 1.7327011124308704 2205 78-79 1.9341741284915024 2205 80-81 1.9888932845379692 2205 82-83 1.8120141101783034 2205 84-85 1.807245817049921 2205 86-87 1.8292809971369692 2205 88-89 1.8140263589667818 2205 90-91 2.011896424151413 2205 92-93 2.0596294973077 2205 94-95 2.087946775980111 2205 96-97 2.0873798672745814 2205 98-99 2.074542413086238 2205 100-101 2.184689102282878 2205 102-103 2.143676693422563 2205 104-105 2.047162587376423 2205 106-107 1.9510735951417857 2205 108-109 2.1235057610557 2205 110-111 2.355602495858541 2205 112-113 2.1766110467302404 2205 114-115 2.3404107456331396 2205 116-117 2.412209980077744 2205 118-119 2.297284936436615 2205 120-121 2.0593406326084285 2206 1 0.8905204540655411 2206 2 1.1727571493024271 2206 3 1.1682128356323283 2206 4 1.2548512019942706 2206 5 1.2901527205425225 2206 6 1.273782996361966 2206 7 1.1393263641083635 2206 8 1.2100151462137276 2206 9 1.25783102547134 2206 10-11 1.456966615020086 2206 12-13 1.52407201829881 2206 14-15 1.8003043172615207 2206 16-17 1.6919059034498858 2206 18-19 1.5856719029442203 2206 20-21 1.7273073014689615 2206 22-23 1.6207247218493208 2206 24-25 1.7994476547024973 2206 26-27 1.8075944661024508 2206 28-29 1.8499585593489698 2206 30-31 1.7404863984022114 2206 32-33 1.8514212887142278 2206 34-35 1.8400760586234952 2206 36-37 1.8305328827682885 2206 38-39 1.7371994561484314 2206 40-41 1.7033235652564045 2206 42-43 1.6707425961783002 2206 44-45 1.7213689559828609 2206 46-47 1.9385998664982722 2206 48-49 2.0188976162294736 2206 50-51 1.9609026759619894 2206 52-53 1.9321890791700227 2206 54-55 1.8472092647916583 2206 56-57 1.7934380744609726 2206 58-59 1.8006562537405912 2206 60-61 1.7568675122554254 2206 62-63 1.7316781400201258 2206 64-65 1.7293489222292315 2206 66-67 1.6236231329925666 2206 68-69 1.7055164843104613 2206 70-71 1.5894757358802032 2206 72-73 1.5457534595448728 2206 74-75 1.5465312927990098 2206 76-77 1.555471011556076 2206 78-79 1.6561453231454557 2206 80-81 1.639209077758121 2206 82-83 1.6011781856796325 2206 84-85 1.6031338722360822 2206 86-87 1.4274855163881668 2206 88-89 1.526740757002134 2206 90-91 1.7029307763708665 2206 92-93 1.7274612446645001 2206 94-95 1.6603122807477497 2206 96-97 1.665992534163422 2206 98-99 1.6413548429144598 2206 100-101 1.6905602892792722 2206 102-103 1.671920934887897 2206 104-105 1.6883805591168155 2206 106-107 1.8084292151933603 2206 108-109 1.868062480343788 2206 110-111 2.00181427145122 2206 112-113 1.8426642462397247 2206 114-115 1.802087706856792 2206 116-117 1.7993476618233224 2206 118-119 1.8428323922412027 2206 120-121 1.7001280017424456 2207 1 0.6864988007592032 2207 2 1.0571062752174853 2207 3 1.0520085908487324 2207 4 1.1876141028396816 2207 5 1.2280321676268002 2207 6 1.1881058945154095 2207 7 1.0578004903788738 2207 8 0.9980913252070636 2207 9 1.1725579088796039 2207 10-11 1.3365518050078506 2207 12-13 1.3650063313579963 2207 14-15 1.408886160294962 2207 16-17 1.3554304046532195 2207 18-19 1.2615722677821424 2207 20-21 1.359367303990311 2207 22-23 1.4143912434907762 2207 24-25 1.4761515350273129 2207 26-27 1.4705594354054483 2207 28-29 1.564938762934041 2207 30-31 1.5399931705107406 2207 32-33 1.5477436674309715 2207 34-35 1.6129901837944658 2207 36-37 1.6641121428364016 2207 38-39 1.586659734881195 2207 40-41 1.5268208718634 2207 42-43 1.5342013147052214 2207 44-45 1.5764350277618107 2207 46-47 1.5546498916644182 2207 48-49 1.7105579558159505 2207 50-51 1.6405061125855056 2207 52-53 1.5220270300870453 2207 54-55 1.603953363545294 2207 56-57 1.5698085389871395 2207 58-59 1.6313876642102727 2207 60-61 1.7351784709478792 2207 62-63 1.512963110763895 2207 64-65 1.3826381982311595 2207 66-67 1.5406420308199351 2207 68-69 1.6254129950797669 2207 70-71 1.496853300954342 2207 72-73 1.431874025730874 2207 74-75 1.5514260831712328 2207 76-77 1.6404335561892438 2207 78-79 1.6582576674447758 2207 80-81 1.633565748775542 2207 82-83 1.6468424058752937 2207 84-85 1.634378131654902 2207 86-87 1.5396270647834598 2207 88-89 1.6954835590597845 2207 90-91 1.7694137444397278 2207 92-93 1.7174746476478475 2207 94-95 1.8779133647400919 2207 96-97 1.7699303003391833 2207 98-99 1.683468315187632 2207 100-101 1.6378102606059883 2207 102-103 1.6930775535824978 2207 104-105 1.8700153913283337 2207 106-107 1.7126339509773345 2207 108-109 1.7486341735083606 2207 110-111 1.8556078181137927 2207 112-113 1.8266271595971126 2207 114-115 1.6707469535408812 2207 116-117 1.7303675113193826 2207 118-119 1.7482073771936548 2207 120-121 1.611371626083553 2208 1 0.7773012724567891 2208 2 0.9078146148658384 2208 3 1.0407812305408015 2208 4 1.1822316027203925 2208 5 1.2814574257229907 2208 6 1.2482170912893835 2208 7 1.1073018124654084 2208 8 1.0322810149614412 2208 9 1.116040469275724 2208 10-11 1.2051536073162836 2208 12-13 1.2125811257320862 2208 14-15 1.467742686619772 2208 16-17 1.3398378186490376 2208 18-19 1.3383217997088437 2208 20-21 1.4160774850347195 2208 22-23 1.2918378074461074 2208 24-25 1.3982461943388813 2208 26-27 1.562661643490145 2208 28-29 1.466535641994426 2208 30-31 1.4746069279989484 2208 32-33 1.5042030697853335 2208 34-35 1.316060408226491 2208 36-37 1.2882581524532668 2208 38-39 1.4497289848651533 2208 40-41 1.5024360859046624 2208 42-43 1.4326409271710645 2208 44-45 1.4026994381210827 2208 46-47 1.4998842647076387 2208 48-49 1.3935538644138177 2208 50-51 1.4021027759144218 2208 52-53 1.4207391406483296 2208 54-55 1.4527895014181738 2208 56-57 1.3262777863750088 2208 58-59 1.2528831476526037 2208 60-61 1.356587021921662 2208 62-63 1.3486521982069881 2208 64-65 1.3120153942815271 2208 66-67 1.487910449945229 2208 68-69 1.5413833945285198 2208 70-71 1.239389174994951 2208 72-73 1.233673527829957 2208 74-75 1.3856518530994713 2208 76-77 1.3663067338753692 2208 78-79 1.191991213121252 2208 80-81 1.255632783489844 2208 82-83 1.2759990530645773 2208 84-85 1.358544836866809 2208 86-87 1.3171128534730236 2208 88-89 1.2679845979530633 2208 90-91 1.4262979641033589 2208 92-93 1.6154453184063406 2208 94-95 1.7413361035315198 2208 96-97 1.6606874501462237 2208 98-99 1.8033982371970474 2208 100-101 1.77655877676656 2208 102-103 1.873629526309653 2208 104-105 1.8472815452732156 2208 106-107 1.8339749030441794 2208 108-109 1.8191132326558979 2208 110-111 1.7472427938887911 2208 112-113 1.8530618972630784 2208 114-115 2.014723328751291 2208 116-117 2.016120020157519 2208 118-119 1.8910189121724095 2208 120-121 1.8270446817534243 2209 1 0.5718191406449371 2209 2 0.7646787189555688 2209 3 0.9201589910544925 2209 4 0.935006911864761 2209 5 1.0243561459853012 2209 6 0.9610572453844242 2209 7 0.8333878546860021 2209 8 0.9060792435803435 2209 9 1.116768867939328 2209 10-11 1.009025237258001 2209 12-13 1.2126165320541986 2209 14-15 1.3904874463673238 2209 16-17 1.2528371984311661 2209 18-19 1.1134825925227219 2209 20-21 1.2659248314710112 2209 22-23 1.25460077295633 2209 24-25 1.2958844618654979 2209 26-27 1.3306349062051481 2209 28-29 1.454339098230669 2209 30-31 1.3493499168698762 2209 32-33 1.2830423365736507 2209 34-35 1.343138817722192 2209 36-37 1.3286967966134782 2209 38-39 1.3255883472169145 2209 40-41 1.4124983290978932 2209 42-43 1.5609405464923825 2209 44-45 1.5776760339816462 2209 46-47 1.5581285520750683 2209 48-49 1.577546881053948 2209 50-51 1.3802277878839107 2209 52-53 1.3741060716853113 2209 54-55 1.351068445876308 2209 56-57 1.3111786248508963 2209 58-59 1.3976567027360076 2209 60-61 1.3334569778636833 2209 62-63 1.3663776400223036 2209 64-65 1.3366239559038924 2209 66-67 1.4198289036736007 2209 68-69 1.4052924225147443 2209 70-71 1.4218408955787218 2209 72-73 1.3827846843433704 2209 74-75 1.4615133547778179 2209 76-77 1.2720402505742143 2209 78-79 1.2106786512477719 2209 80-81 1.2590272128141997 2209 82-83 1.3208917004419902 2209 84-85 1.2965523839252207 2209 86-87 1.3879560461591822 2209 88-89 1.4346605132368495 2209 90-91 1.5786122250131989 2209 92-93 1.6049748562006307 2209 94-95 1.611674742114527 2209 96-97 1.7359957163640196 2209 98-99 1.8159835498512216 2209 100-101 1.8048893462581006 2209 102-103 1.7432174854696285 2209 104-105 1.7798666467973412 2209 106-107 1.9274792629263686 2209 108-109 1.982187091831964 2209 110-111 2.217906180379252 2209 112-113 2.1862664973197106 2209 114-115 2.215929843551926 2209 116-117 2.278296315054998 2209 118-119 2.3981311921592514 2209 120-121 2.2507947381559745 2210 1 0.4027856823063054 2210 2 0.5569120189294949 2210 3 0.6624800906761941 2210 4 0.7271470672871629 2210 5 0.913627762232025 2210 6 0.8550106558456179 2210 7 0.7832367224343031 2210 8 0.8753780595537464 2210 9 0.9202370891904579 2210 10-11 0.9418109622009965 2210 12-13 1.078067472619722 2210 14-15 1.1372127087347579 2210 16-17 1.015577822251153 2210 18-19 1.0386993741356534 2210 20-21 1.1099355941247424 2210 22-23 0.9977434796098947 2210 24-25 1.1224729724755775 2210 26-27 1.1450154574710325 2210 28-29 1.1211578598956748 2210 30-31 1.12157900162601 2210 32-33 1.1025245439249929 2210 34-35 1.2160159969561164 2210 36-37 1.0685772623703826 2210 38-39 0.8828710133288808 2210 40-41 0.865847563401303 2210 42-43 0.973460027884002 2210 44-45 1.064590220437502 2210 46-47 0.8855250584714511 2210 48-49 0.5909147841054576 2210 50-51 0.328909977441036 2210 52-53 0.5252756950275241 2210 54-55 0.5016333884487096 2210 56-57 0.4136047653475927 2210 58-59 0.20770987385744633 2210 60-61 0.4822507511788672 2210 62-63 0.5098182100517796 2210 64-65 0.5720055018814953 2210 66-67 0.6982713560387488 2210 68-69 0.6927161627844818 2210 70-71 0.628986790014455 2210 72-73 0.5993549198406818 2210 74-75 0.4779073175311872 2210 76-77 0.5345801678045632 2210 78-79 0.30659828438344405 2210 80-81 0.2163279766659194 2210 82-83 0.47956904022221636 2210 84-85 0.7602966260600788 2210 86-87 0.7078602495489363 2210 88-89 0.7878095910915626 2210 90-91 0.797273233195785 2210 92-93 0.9225676614195315 2210 94-95 0.9910061178946457 2210 96-97 0.9882442040218464 2210 98-99 0.9286613769804326 2210 100-101 0.9326092596431543 2210 102-103 1.0197128657559738 2210 104-105 1.1114934524334004 2210 106-107 1.0378895608817515 2210 108-109 1.1580486054233425 2210 110-111 1.2351809851669593 2210 112-113 1.2057943189427718 2210 114-115 1.1162194548138054 2210 116-117 1.0255441638853746 2210 118-119 1.222234346240544 2210 120-121 1.2294456654801742 2211 1 0.37154291765235925 2211 2 0.5235800946372748 2211 3 0.6231963767256303 2211 4 0.6574120214564942 2211 5 0.7598143685917336 2211 6 0.6639787862177897 2211 7 0.5315889609362827 2211 8 0.6433666309493802 2211 9 0.6085264204115148 2211 10-11 0.6436219002669148 2211 12-13 0.8635753566850966 2211 14-15 0.9361854697283931 2211 16-17 0.9573461240139594 2211 18-19 0.8149101327409696 2211 20-21 0.7033925836251527 2211 22-23 0.8126154602189288 2211 24-25 0.8524480324478958 2211 26-27 0.9550542509347508 2211 28-29 0.9573317080285833 2211 30-31 0.8932407510594373 2211 32-33 0.9046402191551053 2211 34-35 0.810256493374105 2211 36-37 0.9054167383223728 2211 38-39 0.9139460380431501 2211 40-41 0.6795886444112398 2211 42-43 0.7452754428578174 2211 44-45 0.7430674857192727 2211 46-47 0.8284206091359252 2211 48-49 0.7904887092872954 2211 50-51 0.7611099453533328 2211 52-53 0.8070221598554568 2211 54-55 0.6620399416427389 2211 56-57 0.536880267150373 2211 58-59 0.5217373905089531 2211 60-61 0.5429193860197827 2211 62-63 0.614688672739252 2211 64-65 0.7066910663513646 2211 66-67 0.6496009784701648 2211 68-69 0.5760025494298482 2211 70-71 0.6395466244649342 2211 72-73 0.5843529071535478 2211 74-75 0.6480069527496575 2211 76-77 0.7154093949038796 2211 78-79 0.5416992597921535 2211 80-81 0.4730155739978379 2211 82-83 0.4750028823795098 2211 84-85 0.7245490929227962 2211 86-87 0.7888500112640742 2211 88-89 0.8847698090574134 2211 90-91 1.0307465739778259 2211 92-93 1.10518039831857 2211 94-95 1.0821969850025361 2211 96-97 1.1046471718467146 2211 98-99 1.0317664872843402 2211 100-101 1.087312414710368 2211 102-103 1.1312966247638272 2211 104-105 1.103715305316058 2211 106-107 1.020234740087762 2211 108-109 0.5326986428429912 2211 110-111 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2312 78-79 0.5380846197437563 2312 80-81 0.4014758803279257 2312 82-83 0.5821620420341311 2312 84-85 0.6289292144648932 2312 86-87 0.6302638089505592 2312 88-89 0.6327874170795589 2312 90-91 0.7149314352951741 2312 92-93 0.6469540788626666 2312 94-95 0.6355537147317705 2312 96-97 0.7307591308193828 2312 98-99 0.651269594582363 2312 100-101 0.7876677145325495 2312 102-103 0.7082132501831744 2312 104-105 0.9973928933561673 2312 106-107 1.1539897126812129 2312 108-109 1.040388174273449 2312 110-111 1.1093306416019466 2312 112-113 1.2515072163410323 2312 114-115 1.2024604109174142 2312 116-117 1.4849161899511536 2312 118-119 1.6165721995058426 2312 120-121 1.6400114545058102 2313 1 -0.6734293514578411 2313 2 -0.43257754083070665 2313 3 -0.4271636081171124 2313 4 -0.11081974677527029 2313 5 -2.6856952941593057 2313 6 -7.951009673598257 2313 7 -3.2492577785311454 2313 8 -1.4464056657194675 2313 9 -0.7533565242300568 2313 10-11 -0.4350167025096141 2313 12-13 -0.6267653371038691 2313 14-15 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-0.42165807253069687 2313 80-81 -0.733655341293769 2313 82-83 -0.5082621284717099 2313 84-85 -0.4706522574509684 2313 86-87 -0.4633805168285221 2313 88-89 -0.534559137857741 2313 90-91 -0.40701910861544377 2313 92-93 -0.8779347623827967 2313 94-95 -0.6505564684405734 2313 96-97 -0.5970304608654011 2313 98-99 -0.41368805817020515 2313 100-101 -0.39485541745746033 2313 102-103 -0.32007614040204047 2313 104-105 -0.2991662958414061 2313 106-107 -0.052332347996568274 2313 108-109 -0.21323473412487104 2313 110-111 -0.2573883358815472 2313 112-113 -0.20888572556279783 2313 114-115 -0.23629923133587383 2313 116-117 -0.139187587396723 2313 118-119 -0.04139095550812755 2313 120-121 -0.037099370744758886 2314 1 -0.41334034130539266 2314 2 -0.45923209697746614 2314 3 -0.35756602212400423 2314 4 -0.815010486534284 2314 5 -0.29821422136512865 2314 6 -0.3041644557715628 2314 7 -0.49379340474551725 2314 8 -0.38589360327603117 2314 9 -0.3030836037594824 2314 10-11 -2.698483949729365 2314 12-13 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2314 76-77 -0.19711453899626008 2314 78-79 -0.2305369034018021 2314 80-81 -0.3922954577679292 2314 82-83 -0.30130864408426916 2314 84-85 -0.31578673782384215 2314 86-87 -0.4028659756841009 2314 88-89 -0.42453525557372984 2314 90-91 -0.33434940512695377 2314 92-93 -0.10887146038747275 2314 94-95 -0.034344896439112205 2314 96-97 0.10592813212477026 2314 98-99 -0.011920217642394704 2314 100-101 0.00567237544211352 2314 102-103 0.230991219226798 2314 104-105 0.392874626272544 2314 106-107 0.319758625287065 2314 108-109 0.2899618606357137 2314 110-111 0.4361625798900235 2314 112-113 0.3511193650693549 2314 114-115 0.3477991427292295 2314 116-117 0.44935060489283885 2314 118-119 0.4710673054910295 2314 120-121 0.5002158459696169 2315 1 -0.5620664664324764 2315 2 -3.633341836938648 2315 3 -1.2821401601089946 2315 4 -0.8072146178512014 2315 5 -3.2439689213138507 2315 6 -0.881729557754575 2315 7 -0.6310798919867082 2315 8 -0.38520954398394025 2315 9 -0.22675341937240745 2315 10-11 -0.23268731554180277 2315 12-13 -0.154033338298305 2315 14-15 -0.18804367495521745 2315 16-17 -0.055421941056913226 2315 18-19 -0.20732753957283734 2315 20-21 -0.24375825883210922 2315 22-23 -0.23652808514640355 2315 24-25 -0.08991601614606282 2315 26-27 -0.06865699095524036 2315 28-29 -0.03674032768163471 2315 30-31 -0.16890050858106775 2315 32-33 -0.18189323148953918 2315 34-35 -0.17871157723418207 2315 36-37 -0.20444448772443025 2315 38-39 -0.27844462094086 2315 40-41 -0.11572038989309164 2315 42-43 -0.17890740282345519 2315 44-45 -0.33828909043105426 2315 46-47 -0.45481607567093363 2315 48-49 -0.21686744848001638 2315 50-51 -0.4413057550844357 2315 52-53 -0.7969395580990195 2315 54-55 -0.6254102600117264 2315 56-57 -0.4743000959199506 2315 58-59 -0.8160373656137452 2315 60-61 -0.8568383517586682 2315 62-63 -0.7023488986704223 2315 64-65 -0.5435455046306927 2315 66-67 -0.47767113353342694 2315 68-69 -0.6928560647994821 2315 70-71 -0.627978855368081 2315 72-73 -0.5551789037988577 2315 74-75 -0.6623097262736621 2315 76-77 -0.6296729127245335 2315 78-79 -0.8028305361538379 2315 80-81 -0.6718281630238288 2315 82-83 -0.7644346385780949 2315 84-85 -0.6447131378585063 2315 86-87 -0.49214955497118495 2315 88-89 -0.7433901363066902 2315 90-91 -0.5643528073690582 2315 92-93 -0.6108425942889717 2315 94-95 -0.4431987099593364 2315 96-97 -0.3626928043423696 2315 98-99 -0.17773157615606294 2315 100-101 -0.42625550023482717 2315 102-103 -0.23219315100133642 2315 104-105 -0.10652215780624985 2315 106-107 -0.21299517675861424 2315 108-109 0.030288941279284387 2315 110-111 0.054838040496630214 2315 112-113 -0.10025986697077727 2315 114-115 -0.023604731493730213 2315 116-117 0.12365192157658456 2315 118-119 0.01124806565141867 2315 120-121 -0.13915872724559009 2316 1 -1.6797333922446427 2316 2 -3.7509832730295436 2316 3 -6.011026641635318 2316 4 -2.5612892772448 2316 5 -3.2048878257656312 2316 6 -1.2737283342479557 2316 7 -1.0686259965200549 2316 8 -1.270310037210045 2316 9 -0.9382984788271784 2316 10-11 -0.8213131646681475 2316 12-13 -0.5520448851420383 2316 14-15 -5.1412216059545415 2316 16-17 -1.7470067644768648 2316 18-19 -1.0742927333584475 2316 20-21 -0.6888689479467303 2316 22-23 -1.1710209034174355 2316 24-25 -0.8576504897195889 2316 26-27 -1.019800204954194 2316 28-29 -0.5255388696695817 2316 30-31 -0.3760113761237278 2316 32-33 -0.9126459429586404 2316 34-35 -0.8127036499308673 2316 36-37 -1.3692630646332873 2316 38-39 -1.13075615631422 2316 40-41 -0.8914592731884028 2316 42-43 -0.8209538450956018 2316 44-45 -1.3682670418221576 2316 46-47 -1.1704105596952488 2316 48-49 -1.2650509234937175 2316 50-51 -1.2526338970112008 2316 52-53 -1.3965112033330769 2316 54-55 -1.1228145142462047 2316 56-57 -1.2850185237139407 2316 58-59 -1.5171609525273269 2316 60-61 -1.4083837935592598 2316 62-63 -1.3474832932258174 2316 64-65 -1.1289650017184911 2316 66-67 -0.9237487752244142 2316 68-69 -1.2378398151021734 2316 70-71 -1.0799070253335437 2316 72-73 -1.1329019069978195 2316 74-75 -0.9629917037662388 2316 76-77 -1.0859967851229868 2316 78-79 -0.8906428808258475 2316 80-81 -0.9973874712327948 2316 82-83 -1.065731555596308 2316 84-85 -1.0467899128989693 2316 86-87 -0.8298154611944994 2316 88-89 -0.9199502984213161 2316 90-91 -1.1100416033620775 2316 92-93 -0.9830730723486916 2316 94-95 -0.9888822805594906 2316 96-97 -0.9156663077771121 2316 98-99 -0.7545210183135147 2316 100-101 -0.7328801513442649 2316 102-103 -0.7913276475156188 2316 104-105 -1.0653173861304275 2316 106-107 -1.1801190440591256 2316 108-109 -1.1934455577839707 2316 110-111 -1.1152139585347562 2316 112-113 -1.0776845129822767 2316 114-115 -0.8815112606858371 2316 116-117 -0.889029469652403 2316 118-119 -0.6717626527763763 2316 120-121 -0.8661110487219581 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33975.0 3 5149.0 4 4800.0 5 5676.0 6 6881.0 7 7319.0 8 9816.0 9 12848.0 10 18983.0 11 23849.0 12 23032.0 13 25586.0 14 32237.0 15 32423.0 16 22851.0 17 21454.0 18 21769.0 19 22990.0 20 25791.0 21 28574.0 22 31393.0 23 35034.0 24 39217.0 25 43922.0 26 50426.0 27 57454.0 28 66213.0 29 77772.0 30 91773.0 31 108884.0 32 130763.0 33 159937.0 34 203053.0 35 263243.0 36 351670.0 37 378300.0 38 138115.0 39 825.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.01091040476314 18.77796547953314 12.367944127657694 21.843179988046025 2 16.049400822060555 21.114297292603386 39.57283726674514 23.263464618590916 3 19.99155642358197 26.43584213290606 29.690979767446617 23.881621676065354 4 12.073222864744656 16.925013156534206 39.4885850668321 31.513178911889035 5 10.868943653847118 36.998295674439476 33.867962422264505 18.2647982494489 6 27.070732251335006 37.209700784679036 19.73139387615392 15.988173087832033 7 26.129200768837247 31.929546915353058 23.475230770774 18.466021545035698 8 24.615221997917605 31.399435770803976 22.239680519587736 21.745661711690687 9 24.259908996746027 17.96945855185623 24.598654046828678 33.17197840456906 10-11 23.518935476616537 25.25182176114957 28.873453224394762 22.35578953783913 12-13 26.190387560058703 23.377698964977657 28.4512236825178 21.98068979244584 14-15 23.015293625929633 25.633184041765894 28.504029830796675 22.847492501507798 16-17 20.302969620081242 28.471006809398574 28.50270400414861 22.723319566371572 18-19 19.296095450755224 27.962954707861726 30.88755053328061 21.853399308102443 20-21 21.786019852554972 26.9948359893069 29.21803116158788 22.00111299655025 22-23 20.60415657874176 27.460891004111048 29.470000520874528 22.464951896272662 24-25 21.349869300096277 26.8374326993393 29.887907302701596 21.924790697862832 26-27 21.005645497625945 25.376363293347126 31.677403001647004 21.94058820737993 28-29 20.334617205742582 27.74980039585101 30.372265114442925 21.543317283963482 30-31 21.378000745211555 27.447789363311088 29.53501847881521 21.63919141266215 32-33 20.498265190628366 27.19841889423702 30.470052107954498 21.833263807180114 34-35 20.77606683428111 25.789506286120517 30.979706996986373 22.454719882611997 36-37 22.17340691075566 26.986611631557594 30.348139264969426 20.491842192717318 38-39 21.586861265416733 27.51308478228821 30.33986858486758 20.56018536742748 40-41 20.441545945368045 27.82600926041894 30.191289231487122 21.541155562725898 42-43 20.31357549039226 27.98095182594208 28.93380435330048 22.771668330365177 44-45 21.409500936325614 27.563492832179502 28.764027037487367 22.262979194007514 46-47 21.631674792519213 29.119581513609894 28.309491475523757 20.939252218347136 48-49 21.426535945568677 27.790304733993242 29.438447862212268 21.344711458225813 50-51 20.368206393233464 27.72851244694342 30.295920120290535 21.60736103953258 52-53 20.805642103378364 28.714207212081313 28.432310426823765 22.047840257716565 54-55 20.814583980985603 28.884895716647378 28.2685526686747 22.031967633692314 56-57 21.118281512989547 27.665530291183188 28.35233663588838 22.863851559938883 58-59 21.668465033552682 27.019138856632363 28.763538653372837 22.54885745644211 60-61 21.75409135172644 28.119179770477025 29.171827603944994 20.95490127385154 62-63 20.953820527100476 28.272687871254938 28.97993201965386 21.793559581990728 64-65 20.36099939259084 29.152031347560754 28.926452553009373 21.560516706839024 66-67 20.9165758169241 27.900435113988287 27.74630249587558 23.43668657321203 68-69 21.28817105108258 28.74768652986234 27.850822427778237 22.113319991276843 70-71 22.283868419970744 27.800893225418495 27.925386590501176 21.989851764109584 72-73 21.46170943585972 27.03345001606921 29.98740521337306 21.51743533469801 74-75 20.678118563616323 28.55216957403808 29.37457895072107 21.395132911624533 76-77 20.97315371457916 28.02612425818458 28.5287826311908 22.471939396045453 78-79 21.675963195018504 28.446102024892035 28.441138296803896 21.436796483285566 80-81 21.577258019769385 27.800051158366056 28.53151146697001 22.09117935489455 82-83 20.870612964427853 27.515612253726545 29.53054702370652 22.083227758139078 84-85 21.000707320067736 28.116281565895434 28.768757301960036 22.114253812076797 86-87 21.604733107432565 28.473262876382254 27.99509596150268 21.9269080546825 88-89 20.79351207125635 27.513091895338952 28.151744003359923 23.541652030044773 90-91 20.71278099173474 26.056601890564686 28.879978735056287 24.350638382644284 92-93 21.656908068446068 27.237683629951487 28.71199242164556 22.39341587995689 94-95 20.892741792759566 26.70930096338251 29.520879463607642 22.877077780250282 96-97 19.18001236468764 27.22705762704929 30.479591992330263 23.113338015932804 98-99 20.11025352789278 28.09279276113435 28.901264569464434 22.89568914150844 100-101 19.86845039234178 27.590435528819192 28.8613159401096 23.67979813872943 102-103 20.247465158413487 27.386209820977932 28.869101558300535 23.49722346230805 104-105 21.210282561631722 27.052727829234847 29.378350515066003 22.358639094067428 106-107 20.543342833499047 27.03208853372199 30.076458064436444 22.348110568342523 108-109 20.588369250269896 26.849603545415214 29.441312254447304 23.12071494986759 110-111 20.618265433236424 27.447283787056705 28.59266792814404 23.34178285156284 112-113 21.125451657736612 26.363590919539543 28.0164927330415 24.494464689682353 114-115 20.651270535926944 26.507602138720358 28.511890079254325 24.329237246098373 116-117 22.012597315468636 26.680419138266398 27.355577328693403 23.951406217571567 118-119 22.1475250676705 26.483166884786304 27.86638452365393 23.502923523889265 120-121 21.74773794411406 27.382577590461487 27.519654924892162 23.350029540532294 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6068.0 1 6749.5 2 4554.5 3 1625.5 4 1549.5 5 1500.0 6 1494.0 7 1654.0 8 1910.0 9 2027.0 10 1984.0 11 1991.0 12 2146.5 13 2373.0 14 2645.0 15 2903.5 16 3154.5 17 3498.5 18 3923.5 19 4467.0 20 5089.0 21 5842.0 22 6947.0 23 8629.5 24 10925.5 25 13794.0 26 17563.0 27 22152.5 28 27513.5 29 33495.5 30 39382.0 31 45264.0 32 51405.5 33 57139.5 34 62022.0 35 66298.0 36 69895.5 37 73889.0 38 77498.5 39 79380.5 40 80380.0 41 80636.0 42 80435.5 43 81286.0 44 83049.0 45 86603.0 46 93481.5 47 112628.5 48 153158.0 49 156574.0 50 124616.0 51 102822.0 52 82780.5 53 70673.5 54 63869.0 55 57910.5 56 51943.5 57 46300.5 58 40381.5 59 34216.5 60 28306.5 61 22611.0 62 17007.0 63 11831.0 64 7832.0 65 5120.5 66 3159.0 67 1852.5 68 1074.5 69 611.5 70 388.5 71 260.0 72 186.5 73 137.0 74 99.5 75 72.5 76 66.5 77 55.0 78 40.0 79 35.5 80 31.0 81 25.5 82 28.0 83 23.0 84 16.5 85 12.0 86 8.5 87 7.0 88 6.0 89 7.0 90 6.5 91 4.5 92 3.5 93 4.5 94 4.5 95 2.0 96 1.0 97 1.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47253305952531693 2 0.8778510457357067 3 0.8675602917677411 4 0.8465579723312612 5 0.8552419914789496 6 0.8653032119011613 7 0.8428089244172812 8 0.8335128158142493 9 0.8332832822684954 10-11 0.8258616976224533 12-13 0.8277553493749227 14-15 0.8279466273297178 16-17 0.8525258445208621 18-19 0.880127253397766 20-21 0.8719979403189828 22-23 0.8493506304712668 24-25 0.8412595729834426 26-27 0.8423881129167325 28-29 0.8652649563102024 30-31 0.8717110233867904 32-33 0.8663361128570537 34-35 0.8772580840758425 36-37 0.8862290201557232 38-39 0.8729543300929572 40-41 0.8828434003558535 42-43 0.8753835601188524 44-45 0.860138707121699 46-47 0.8558349531388139 48-49 0.8597752790075887 50-51 0.9029084578138384 52-53 0.8654562342649972 54-55 0.876339949892827 56-57 0.9251732117519645 58-59 0.8883139498629875 60-61 0.9037692086104153 62-63 0.8781188348724196 64-65 0.8669864579033564 66-67 0.8692435377699361 68-69 0.8872236655206567 70-71 0.898910748558625 72-73 0.9043430424748 74-75 0.9082642405480954 76-77 0.9206973076097639 78-79 0.9646912372125905 80-81 0.9929238633403176 82-83 0.9565810519292869 84-85 0.9158005919670145 86-87 0.8982030201258838 88-89 0.8975909306705401 90-91 0.9111907932564575 92-93 0.8975144194886222 94-95 0.8906475409114853 96-97 0.9011295728342459 98-99 0.9342015312182838 100-101 0.9219406143159307 102-103 0.8971127357835529 104-105 0.8861525089738053 106-107 0.8861907645647642 108-109 0.8859229754280514 110-111 0.8844501351761307 112-113 0.895238211826563 114-115 0.898088253353007 116-117 0.9242359497734696 118-119 0.9203338794956537 120-121 0.8985664482399942 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2613997.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.732496770825826 #Duplication Level Percentage of deduplicated Percentage of total 1 81.40298199464956 50.25209323120296 2 12.32076911985893 15.211836798115641 3 2.947568037290483 5.458822030314725 4 1.1164433842921975 2.756833504625118 5 0.5701115892665493 1.7597205921703816 6 0.3454716863506431 1.27960978572317 7 0.23424592145229575 1.0122409912743078 8 0.17231764429051016 0.8510078735776185 9 0.12200852960946643 0.6778702044116635 >10 0.6868978078885354 7.769871470923177 >50 0.05067532188311509 2.1701139586332037 >100 0.025307295080913225 2.975680467350319 >500 0.0025068279936425308 1.0611009255730508 >1k 0.0021934744944300828 3.345483659782437 >5k 3.133534992042976E-4 1.3102528547159435 >10k+ 1.8801209952257856E-4 2.107461651606035 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 24923 0.9534440934706505 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 18126 0.6934208417224657 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11424 0.4370318711153839 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 8660 0.331293417704764 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7782 0.2977050088427799 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6399 0.244797526546511 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5709 0.218401168784815 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5317 0.20340497712889494 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4785 0.18305300273871775 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4480 0.17138504749622896 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4119 0.1575747791600373 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 4055 0.15512642133866258 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3828 0.1464424021909742 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3699 0.1415074309572658 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3615 0.13829396131671154 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3508 0.1342006130841007 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3487 0.13339724567396213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3404 0.13022203162436682 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3197 0.12230312429585802 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2758 0.10550891986486595 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2665 0.10195114990568083 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 7.651118191795936E-5 0.0 0.0 0.0 3 0.0 7.651118191795936E-5 0.0 0.0 0.0 4 0.0 7.651118191795936E-5 0.0 0.0 0.0 5 0.0 1.1476677287693903E-4 0.0 0.0 0.0 6 0.0 1.1476677287693903E-4 0.0 0.0 0.0 7 0.0 1.1476677287693903E-4 0.0 0.0 0.0 8 0.0 1.1476677287693903E-4 0.0 0.0 0.0 9 0.0 1.1476677287693903E-4 0.0 3.825559095897968E-5 0.0 10-11 0.0 1.1476677287693903E-4 0.0 1.3389456835642889E-4 0.0 12-13 0.0 1.7215015931540856E-4 0.0 1.912779547948984E-4 0.0 14-15 0.0 2.1040575027438823E-4 0.0 2.1040575027438823E-4 0.0 16-17 0.0 2.2953354575387807E-4 0.0 3.2517252315132725E-4 0.0 18-19 0.0 2.2953354575387807E-4 0.0 4.016837050692866E-4 0.0 20-21 0.0 2.2953354575387807E-4 0.0 6.312172508231646E-4 0.0 22-23 0.0 2.2953354575387807E-4 0.0 0.0013580734790437786 0.0 24-25 0.0 2.486613412333679E-4 0.0 0.0024292300258952097 0.0 26-27 0.0 2.677891367128577E-4 0.0 0.004380265164803173 0.0 28-29 0.0 3.0604472767183744E-4 0.0 0.009353491989470532 0.0 30-31 0.0 3.0604472767183744E-4 0.0 0.018860006342776982 0.0 32-33 0.0 3.0604472767183744E-4 0.0 0.036572344956784575 0.0 34-35 0.0 3.825559095897968E-4 0.0 0.056771296983125843 0.0 36-37 0.0 4.2081150054877647E-4 0.0 0.08121661960591386 0.0 38-39 3.825559095897968E-5 4.5906709150775614E-4 0.0 0.1193765715874961 0.0 40-41 3.825559095897968E-5 4.7819488698724595E-4 0.0 0.16478595805580495 0.0 42-43 3.825559095897968E-5 4.973226824667358E-4 0.0 0.20757483654342374 0.0 44-45 3.825559095897968E-5 4.973226824667358E-4 0.0 0.2345450281695044 0.0 46-47 3.825559095897968E-5 4.973226824667358E-4 0.0 0.26949151051053233 0.0 48-49 3.825559095897968E-5 4.973226824667358E-4 0.0 0.2962704241818181 0.0 50-51 3.825559095897968E-5 4.973226824667358E-4 0.0 0.3240439832180374 0.0 52-53 3.825559095897968E-5 4.973226824667358E-4 0.0 0.3938604367181753 0.0 54-55 3.825559095897968E-5 4.973226824667358E-4 0.0 0.49950325115139765 0.0 56-57 3.825559095897968E-5 4.973226824667358E-4 0.0 0.6643848481846001 0.0 58-59 3.825559095897968E-5 4.973226824667358E-4 0.0 0.8870515153613412 0.0 60-61 3.825559095897968E-5 4.973226824667358E-4 0.0 1.134852105798132 0.0 62-63 3.825559095897968E-5 4.973226824667358E-4 0.0 1.2456785528062961 0.0 64-65 3.825559095897968E-5 4.973226824667358E-4 0.0 1.4090873095875778 0.0 66-67 3.825559095897968E-5 4.973226824667358E-4 0.0 1.8689960240964316 0.0 68-69 3.825559095897968E-5 4.973226824667358E-4 0.0 2.3590310164854817 0.0 70-71 3.825559095897968E-5 4.973226824667358E-4 0.0 2.698625897428345 0.0 72-73 3.825559095897968E-5 5.355782734257154E-4 0.0 3.100271346906672 0.0 74-75 3.825559095897968E-5 5.355782734257154E-4 0.0 3.402509643278091 0.0 76-77 3.825559095897968E-5 5.355782734257154E-4 0.0 3.5956429942345 0.0 78-79 3.825559095897968E-5 5.355782734257154E-4 0.0 3.7499277925720653 0.0 80-81 3.825559095897968E-5 5.355782734257154E-4 0.0 3.9178698368819855 0.0 82-83 3.825559095897968E-5 5.547060689052053E-4 0.0 4.212973465539555 0.0 84-85 3.825559095897968E-5 5.738338643846952E-4 0.0 4.858287901631104 0.0 86-87 3.825559095897968E-5 5.738338643846952E-4 0.0 5.884321978946418 0.0 88-89 3.825559095897968E-5 5.738338643846952E-4 0.0 7.082008892894674 0.0 90-91 3.825559095897968E-5 5.738338643846952E-4 0.0 8.32137527319274 0.0 92-93 3.825559095897968E-5 5.738338643846952E-4 0.0 9.438381145808506 0.0 94-95 3.825559095897968E-5 5.738338643846952E-4 0.0 10.448041830193379 0.0 96-97 3.825559095897968E-5 5.738338643846952E-4 0.0 11.658448728135495 0.0 98-99 3.825559095897968E-5 5.738338643846952E-4 0.0 12.79961300644186 0.0 100-101 3.825559095897968E-5 5.738338643846952E-4 0.0 13.668378349324808 0.0 102-103 3.825559095897968E-5 5.738338643846952E-4 0.0 14.593742839031567 0.0 104-105 3.825559095897968E-5 5.738338643846952E-4 0.0 15.6836446254529 0.0 106-107 3.825559095897968E-5 5.738338643846952E-4 0.0 16.76270095183736 0.0 108-109 3.825559095897968E-5 5.738338643846952E-4 0.0 17.807384629745176 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4750 0.0 65.52764 2 TATAACG 630 0.0 62.09966 2 TAACGCA 720 0.0 54.257492 4 GTACCTG 6170 0.0 51.399223 1 ACCTGGG 6015 0.0 50.334602 3 ATAACGC 815 0.0 47.933926 3 GTATAAC 1045 0.0 42.48674 1 TACACGG 430 0.0 41.477646 2 GTACACG 475 0.0 41.272835 1 CCTGGGG 3675 0.0 38.9247 4 TATCACG 425 0.0 33.843243 2 TCACGCA 460 0.0 32.471237 4 ACACGGG 580 0.0 29.715631 3 CTGGGGG 2890 0.0 28.029886 5 GAGTACG 885 0.0 28.015875 1 GTCTCGC 855 0.0 26.975712 1 TACGCAG 730 0.0 26.75712 4 GTACCCG 410 0.0 26.720758 1 AGTACGC 805 0.0 26.443974 2 TCTGTCG 600 0.0 25.83614 8 >>END_MODULE