FastQCFastQC Report
Wed 25 May 2016
SRR1003865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2613997
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT306871.1739493197582094No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT148710.5688988931509869No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC101400.3879116923240539No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98750.3777739607199243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81190.310597142995956No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT79950.30585344971704254No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT69990.26775088112189876No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC62780.2401686000404744No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC53370.20417008894807456No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT52850.2021807982182076No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50480.1931142231609294No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG50470.19307596756997042No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA49280.18852355224585185No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46120.17643478550281427No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA41640.15929628075319138No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC37840.1447591561887791No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT33390.12773541821203316No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA32060.12264742461448885No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC31510.12054336711174497No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA28710.10983180164323066No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27080.10359614031691697No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG44000.057.2375952
TATAACG6950.055.3806952
ATAACGC7700.051.2206953
TAACGCA8000.049.8662264
GTACCTG53000.048.622731
TATCACG4550.048.0401762
ACCTGGG56700.043.1600883
TCACGCA4950.042.2147944
GTATAAC10700.040.4364971
CCTGGGG31750.039.937854
GTACCCG3150.033.2068751
GCGATCG650.00583460429.2418482
GTACACG3500.028.5277251
CTGGGGG21850.026.0823675
TACACGG3550.025.4321692
CACGCAG8450.024.7293745
ATCACGC8800.024.2989793
GTACCGG2202.1482265E-923.7731061
TCGACTC5400.022.8663464
ACAACGC2955.456968E-1222.5509153
Produced by FastQC (version 0.11.5)