##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003865_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2613997 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.597297548543477 31.0 31.0 34.0 30.0 34.0 2 31.79837199507115 31.0 31.0 34.0 30.0 34.0 3 31.82565052676036 31.0 31.0 34.0 30.0 34.0 4 35.6068966414269 37.0 35.0 37.0 33.0 37.0 5 35.474922886292525 37.0 35.0 37.0 33.0 37.0 6 35.51483303156048 37.0 35.0 37.0 33.0 37.0 7 35.44040448401433 37.0 35.0 37.0 33.0 37.0 8 35.431978307549706 37.0 35.0 37.0 33.0 37.0 9 36.98885767657729 39.0 37.0 39.0 33.0 39.0 10-11 36.903173569059184 39.0 37.0 39.0 33.0 39.0 12-13 36.85132289746316 39.0 37.0 39.0 32.0 39.0 14-15 37.924996853477644 40.0 37.0 41.0 33.0 41.0 16-17 37.93065007343161 40.0 37.0 41.0 33.0 41.0 18-19 37.962464953096735 40.0 37.0 41.0 33.0 41.0 20-21 37.893536411862755 40.0 37.0 41.0 32.5 41.0 22-23 37.7712095691005 39.5 37.0 41.0 32.0 41.0 24-25 37.66629992306801 39.0 36.5 41.0 32.0 41.0 26-27 37.4371820243099 39.0 36.0 41.0 32.0 41.0 28-29 37.23389162267593 39.0 36.0 41.0 31.0 41.0 30-31 36.9554507904944 39.0 36.0 40.0 31.0 41.0 32-33 36.616616239421845 38.5 35.0 40.0 30.0 41.0 34-35 36.53282750515781 39.0 35.0 40.0 30.0 41.0 36-37 36.286376763248 38.5 35.0 40.0 30.0 41.0 38-39 35.99514613061912 38.0 35.0 40.0 30.0 41.0 40-41 36.07645743281266 39.0 35.0 40.0 30.0 41.0 42-43 35.89692490083195 39.0 35.0 40.0 29.0 41.0 44-45 35.67503826515485 39.0 35.0 40.0 28.5 41.0 46-47 35.30378037924297 38.0 35.0 40.0 26.5 41.0 48-49 34.99176911832723 38.0 34.0 40.0 25.5 41.0 50-51 34.71939122347884 38.0 34.0 40.0 24.0 41.0 52-53 34.50258875584019 38.0 34.0 40.0 24.0 41.0 54-55 34.2574886275692 38.0 33.5 40.0 23.0 41.0 56-57 33.98925017894052 38.0 33.0 40.0 22.5 41.0 58-59 33.71669592581782 38.0 33.0 40.0 22.0 41.0 60-61 33.24813360535609 37.0 32.0 40.0 19.5 41.0 62-63 32.96625359554736 36.5 32.0 40.0 19.0 41.0 64-65 32.507653604805206 36.0 31.5 39.0 16.5 41.0 66-67 32.15528977271206 36.0 31.0 39.0 13.5 40.0 68-69 31.865145598866413 35.0 31.0 39.0 13.0 40.0 70-71 31.432722761349766 35.0 31.0 38.0 9.5 40.0 72-73 31.03516989499223 35.0 30.0 37.0 7.5 40.0 74-75 30.6588557676233 35.0 30.0 37.0 7.0 39.0 76-77 29.69965306004559 34.0 29.0 36.0 6.0 39.0 78-79 30.0617208053414 34.0 30.0 36.0 2.0 39.0 80-81 29.865437489025425 34.0 30.0 36.0 2.0 37.5 82-83 29.562506957735607 34.0 29.5 35.0 2.0 37.0 84-85 29.21554079824881 34.0 29.0 35.0 2.0 37.0 86-87 28.865588981165622 34.0 29.0 35.0 2.0 36.0 88-89 28.540929082933147 34.0 29.0 35.0 2.0 36.0 90-91 28.2377959882892 34.0 29.0 35.0 2.0 36.0 92-93 27.962855160124516 34.0 28.0 35.0 2.0 35.0 94-95 27.6237069132061 34.0 27.0 35.0 2.0 35.0 96-97 27.242608350353883 34.0 27.0 35.0 2.0 35.0 98-99 26.856537134510866 34.0 26.0 35.0 2.0 35.0 100-101 25.88550082498182 32.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.06970129336590958 1101 2 0.014596084839094914 1101 3 0.05687162939524271 1101 4 0.030497164062467164 1101 5 0.0650794251661182 1101 6 0.14112019624163707 1101 7 -0.018454213166421596 1101 8 0.09496130534449065 1101 9 -0.009999055478310481 1101 10-11 0.07743332117436807 1101 12-13 0.04546009822291097 1101 14-15 0.046319666084940536 1101 16-17 -0.013181897538281362 1101 18-19 -0.13434617024099538 1101 20-21 -0.05402169466955087 1101 22-23 -0.09551991499811407 1101 24-25 -0.0040993926434254035 1101 26-27 -0.024604183235368282 1101 28-29 -0.0833902981913397 1101 30-31 0.12189145339087304 1101 32-33 -0.007629795696558972 1101 34-35 -0.05267247651779883 1101 36-37 -0.04684151330089037 1101 38-39 -0.029220553115997916 1101 40-41 -0.033528600763403915 1101 42-43 -0.03672111736731409 1101 44-45 0.0018842642303127377 1101 46-47 0.08374154420959457 1101 48-49 -0.04760373947816987 1101 50-51 -0.037390006196730496 1101 52-53 0.18348814831367832 1101 54-55 0.25112003653056547 1101 56-57 0.06557204461469723 1101 58-59 -0.18610688340799442 1101 60-61 -0.260419893015289 1101 62-63 -0.3064724387056117 1101 64-65 -0.5144298064652659 1101 66-67 -0.5636292881206479 1101 68-69 -0.7030749297794117 1101 70-71 -0.5068685083688074 1101 72-73 -0.4683289738937475 1101 74-75 -0.5056671798999659 1101 76-77 -0.23485381647764925 1101 78-79 -0.3257779561809748 1101 80-81 -0.35141812230896363 1101 82-83 -0.4689891369222927 1101 84-85 -0.5591964598721653 1101 86-87 -0.6187112564143185 1101 88-89 -0.5074611441185262 1101 90-91 -0.4506767415764479 1101 92-93 -0.4257022395455117 1101 94-95 -0.4676853706422861 1101 96-97 -0.43090322041476625 1101 98-99 -0.7020840567400946 1101 100-101 -0.5489136788837996 1102 1 0.022397627287883637 1102 2 0.006108585491844565 1102 3 0.008929949037579377 1102 4 0.01650472792144342 1102 5 -0.06728408995959256 1102 6 0.054523701807077884 1102 7 -0.0729768625656888 1102 8 -0.05865974652598993 1102 9 0.06945510544750277 1102 10-11 -0.053261038468448874 1102 12-13 0.02002082299565444 1102 14-15 0.030528026699840893 1102 16-17 0.04013379315090049 1102 18-19 -0.03035503457758182 1102 20-21 -0.18408050236757845 1102 22-23 -0.12350754891215843 1102 24-25 -0.19978047636807617 1102 26-27 -0.2646123476237747 1102 28-29 -0.14711633726792428 1102 30-31 -0.2899605171088808 1102 32-33 -0.13454649382909878 1102 34-35 -0.21737143854586094 1102 36-37 -0.29171404138919854 1102 38-39 -0.21845849834128472 1102 40-41 -0.17017013490521293 1102 42-43 0.07615205443230622 1102 44-45 0.08033218688051136 1102 46-47 0.05024696504214177 1102 48-49 -0.1171735173827031 1102 50-51 0.011852528358815562 1102 52-53 0.08620853156794084 1102 54-55 -0.01523999693037581 1102 56-57 0.1199178240376284 1102 58-59 -0.03235854540834282 1102 60-61 -0.0838690106989759 1102 62-63 -0.23251731472340964 1102 64-65 -0.2696941046893784 1102 66-67 -0.1865329583797788 1102 68-69 -0.15275774379203355 1102 70-71 -0.1562230898660779 1102 72-73 -0.13255946271265628 1102 74-75 -0.10505354527090915 1102 76-77 -0.15793470819355804 1102 78-79 -0.03512397159791192 1102 80-81 0.05115539233471367 1102 82-83 -0.041318287303699464 1102 84-85 -0.03381053435388637 1102 86-87 -0.15593893439396922 1102 88-89 -0.022869669828818218 1102 90-91 0.13202647939026235 1102 92-93 -0.010601460062332535 1102 94-95 0.009131230883337338 1102 96-97 0.0176359013661731 1102 98-99 -0.01502340728458762 1102 100-101 -0.08726621978608051 1103 1 0.02913302419668895 1103 2 -0.010500006622528701 1103 3 0.03302776034637134 1103 4 -0.01754656118765041 1103 5 0.11473010650546911 1103 6 -0.006853384497752302 1103 7 -0.02294516658023582 1103 8 -0.012967481180545803 1103 9 -0.05213966137847592 1103 10-11 -0.050091179265351116 1103 12-13 -0.021934913143603296 1103 14-15 0.025440768903052913 1103 16-17 -0.0259385216569612 1103 18-19 -0.004929171904606733 1103 20-21 0.07237442677521244 1103 22-23 0.007127350870135274 1103 24-25 -0.018183071685413665 1103 26-27 -0.0450827202184243 1103 28-29 -0.153837397999105 1103 30-31 -0.017553853444987055 1103 32-33 -0.144319335791252 1103 34-35 -0.22599660432420876 1103 36-37 -0.2102107862949012 1103 38-39 -0.09966502062439986 1103 40-41 -0.035498688817583 1103 42-43 -0.014358230080866008 1103 44-45 -0.05873082140215047 1103 46-47 -0.06735243940698155 1103 48-49 0.030550106135713406 1103 50-51 0.07356091069581794 1103 52-53 -0.03747019417003372 1103 54-55 -0.03583117919063028 1103 56-57 -0.16993075464765894 1103 58-59 -0.17586159823220981 1103 60-61 0.05095830873816709 1103 62-63 -0.1903238586932403 1103 64-65 -0.21107347435632562 1103 66-67 -0.3400457910833623 1103 68-69 -0.37587610744508027 1103 70-71 -0.24318894995332485 1103 72-73 -0.34831893605883124 1103 74-75 -0.38941293187017934 1103 76-77 -0.2833692247046642 1103 78-79 -0.327748294930327 1103 80-81 -0.20233872514061346 1103 82-83 -0.35433595364506587 1103 84-85 -0.34994671031664737 1103 86-87 -0.42933010247444514 1103 88-89 -0.3197987563171516 1103 90-91 -0.2898713281650558 1103 92-93 -0.34712133469636797 1103 94-95 -0.5749272523803448 1103 96-97 -0.38819704619365325 1103 98-99 -0.4449966533705485 1103 100-101 -0.43111865829169815 1104 1 -0.028612202153187383 1104 2 -0.08451255737926644 1104 3 -0.1158111268646067 1104 4 -0.03241283186787314 1104 5 -0.11502592557614122 1104 6 -0.09466673393900749 1104 7 -0.017642975231531466 1104 8 -0.09934621872207572 1104 9 -0.20188461926296952 1104 10-11 -0.07395523510615476 1104 12-13 -0.20311968444465123 1104 14-15 -0.12892682732501015 1104 16-17 -0.06131722564031605 1104 18-19 -0.12851972863469996 1104 20-21 -0.124398115927562 1104 22-23 -0.11510409984968817 1104 24-25 -0.01926951753929984 1104 26-27 -0.00778281082548915 1104 28-29 -0.017020726298703437 1104 30-31 -0.1667409067018042 1104 32-33 -0.05802449368245277 1104 34-35 0.09432454739229001 1104 36-37 0.02064427595551166 1104 38-39 0.057500488285292306 1104 40-41 0.08922445625439934 1104 42-43 0.010822984053838525 1104 44-45 0.3349317176092299 1104 46-47 0.24113945629422062 1104 48-49 0.08131942725387376 1104 50-51 0.2154196979034353 1104 52-53 0.2403117783793789 1104 54-55 0.1522623809959498 1104 56-57 -0.0066870216364876 1104 58-59 0.06962063271514296 1104 60-61 0.12753805973652987 1104 62-63 0.09713545202188101 1104 64-65 -0.005357678524248399 1104 66-67 -0.010672322175004467 1104 68-69 -0.017265080229400098 1104 70-71 0.2241631423987691 1104 72-73 0.18932494460830185 1104 74-75 0.07675623488038141 1104 76-77 0.0995253355154766 1104 78-79 0.06795709786808857 1104 80-81 0.1331674999004946 1104 82-83 0.17327403326725133 1104 84-85 0.09541219434457915 1104 86-87 0.10605699105218491 1104 88-89 0.004691998406162412 1104 90-91 0.10894239041610376 1104 92-93 -0.035735046565893214 1104 94-95 0.01347336509003938 1104 96-97 0.1662594791931049 1104 98-99 0.30720979651031755 1104 100-101 0.25838853993642985 1105 1 0.08332590521485272 1105 2 0.1352027791093171 1105 3 0.026003414873063235 1105 4 0.13854001680230255 1105 5 0.05892819763418089 1105 6 0.13771106546081313 1105 7 0.08609343174278195 1105 8 0.1876275407003476 1105 9 0.1785098098267568 1105 10-11 0.1799570194987936 1105 12-13 0.1703585916726027 1105 14-15 0.1899929903023434 1105 16-17 0.20348167271055928 1105 18-19 0.1700438602696579 1105 20-21 0.14947416946704095 1105 22-23 0.1536174844198399 1105 24-25 0.09155442313199558 1105 26-27 0.24945940069493133 1105 28-29 0.18706107875117794 1105 30-31 0.2852148302263444 1105 32-33 0.2887966562604518 1105 34-35 0.33617327568076405 1105 36-37 0.3344750843989601 1105 38-39 0.34697622709749254 1105 40-41 0.45586158246459263 1105 42-43 0.5867693473906996 1105 44-45 0.518875583574399 1105 46-47 0.33745939121306634 1105 48-49 0.36331610612837295 1105 50-51 0.3384658104535987 1105 52-53 0.3377124741928057 1105 54-55 0.27995445987870227 1105 56-57 0.3158317272099893 1105 58-59 0.23229428209040037 1105 60-61 0.3478309582261474 1105 62-63 0.26385901094549524 1105 64-65 0.2380750735481243 1105 66-67 0.28704709826887864 1105 68-69 0.18916575427313376 1105 70-71 0.256466745080143 1105 72-73 0.22144334347460415 1105 74-75 0.17138700093302361 1105 76-77 0.19109972665313535 1105 78-79 0.3033085896227412 1105 80-81 0.3582554109166516 1105 82-83 0.2584705400186742 1105 84-85 0.24796772541108325 1105 86-87 0.4317022541990312 1105 88-89 0.36360067308663346 1105 90-91 0.5000804966578158 1105 92-93 0.4656059852049417 1105 94-95 0.4588362557747345 1105 96-97 0.524994597292217 1105 98-99 0.4589402298616463 1105 100-101 0.5507545205938307 1106 1 -0.08401583895217257 1106 2 0.037860041737520334 1106 3 0.03953101684962945 1106 4 0.07568303204194393 1106 5 0.013622505397869134 1106 6 -0.030165657810030666 1106 7 0.03036788673281876 1106 8 0.13609066787760327 1106 9 0.03181767257886037 1106 10-11 -0.11102386519803531 1106 12-13 -0.17473219094087966 1106 14-15 -0.07755833409605373 1106 16-17 0.01592802020957862 1106 18-19 -0.05888085085628347 1106 20-21 0.037649767050552896 1106 22-23 8.532195960100353E-4 1106 24-25 -0.07224200074433895 1106 26-27 -0.007642682778389087 1106 28-29 0.03658029241858429 1106 30-31 0.007557296666163893 1106 32-33 0.12845668698477652 1106 34-35 0.15823177990417037 1106 36-37 0.1088290327449215 1106 38-39 0.29197824601886424 1106 40-41 0.04460251628361789 1106 42-43 0.11064176991913399 1106 44-45 0.13376362109229945 1106 46-47 0.039907417852880656 1106 48-49 -0.014490063904460726 1106 50-51 0.2340363211712173 1106 52-53 0.20442267272283488 1106 54-55 0.20247855911910762 1106 56-57 0.31798898327207326 1106 58-59 0.19513114776054152 1106 60-61 0.22633173411158936 1106 62-63 0.03128411291473299 1106 64-65 0.1270422387593868 1106 66-67 0.15266120162362995 1106 68-69 0.14794316637419413 1106 70-71 0.2844474526830787 1106 72-73 0.30005671231681674 1106 74-75 0.29111337365612755 1106 76-77 0.10408153774610085 1106 78-79 0.20179910961707392 1106 80-81 0.24139396859207807 1106 82-83 0.252953153644043 1106 84-85 0.1461922858223481 1106 86-87 0.30233012918578694 1106 88-89 0.2113720248536275 1106 90-91 0.13246009416636184 1106 92-93 0.07943184185680607 1106 94-95 0.12260794515485074 1106 96-97 0.26316210516549887 1106 98-99 0.08520887683497946 1106 100-101 0.046344194171155095 1107 1 -0.10594320592746342 1107 2 0.007802599539502353 1107 3 -0.03587325344281922 1107 4 -0.09080078300416972 1107 5 -0.03598978063614311 1107 6 -0.0172482966498535 1107 7 -0.04602396921175256 1107 8 0.04768468170804141 1107 9 -0.05931432909627432 1107 10-11 0.06482164131839596 1107 12-13 -0.04385597220523607 1107 14-15 -0.01779395164796682 1107 16-17 0.00596886807790753 1107 18-19 -0.014953536960916836 1107 20-21 0.015090611903538331 1107 22-23 -0.06765228021939862 1107 24-25 -0.05893806571516791 1107 26-27 -0.01529834059378743 1107 28-29 -0.034266518044837824 1107 30-31 -0.044224600163659034 1107 32-33 -0.049697858581907894 1107 34-35 -0.1950550574082186 1107 36-37 -0.04314072465614771 1107 38-39 0.053593373448684645 1107 40-41 0.06231594773507254 1107 42-43 0.1765841697273487 1107 44-45 -0.03305530770746401 1107 46-47 0.012414882373128933 1107 48-49 -0.008857548687274175 1107 50-51 0.00779024188999955 1107 52-53 0.028520036756297884 1107 54-55 -0.028938887953295023 1107 56-57 -0.0932568960249256 1107 58-59 0.056190206902662965 1107 60-61 0.01316019985379313 1107 62-63 -0.050980862321495124 1107 64-65 -0.13379394699693137 1107 66-67 -0.19144430382372946 1107 68-69 -0.1864568117957539 1107 70-71 0.031219327250479267 1107 72-73 0.06062675084212543 1107 74-75 0.1417101427832499 1107 76-77 -0.013944604241704184 1107 78-79 -0.07746303705721047 1107 80-81 -0.03564785477562182 1107 82-83 0.03655146919124164 1107 84-85 -0.01944636224441254 1107 86-87 0.08894253720394474 1107 88-89 -0.045037220373508546 1107 90-91 0.0353779699276906 1107 92-93 -0.08934848986282162 1107 94-95 0.16545926915633302 1107 96-97 0.2679928767694335 1107 98-99 0.017155677791365065 1107 100-101 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-0.7771223102666518 2316 88-89 -0.6867395932579932 2316 90-91 -0.8454268837206129 2316 92-93 -0.611926529501563 2316 94-95 -0.7409301383832343 2316 96-97 -0.6484279264245352 2316 98-99 -0.845833594207491 2316 100-101 -1.4605775327505732 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 12.0 4 35.0 5 109.0 6 241.0 7 612.0 8 1420.0 9 2683.0 10 4642.0 11 8405.0 12 13635.0 13 18149.0 14 18012.0 15 20724.0 16 25182.0 17 31390.0 18 27431.0 19 18793.0 20 16971.0 21 16727.0 22 17650.0 23 19590.0 24 22814.0 25 27326.0 26 33304.0 27 41328.0 28 51650.0 29 63304.0 30 77928.0 31 94285.0 32 115670.0 33 142955.0 34 181557.0 35 238400.0 36 329369.0 37 446777.0 38 397727.0 39 87134.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.86331279457955 19.984636124169512 12.996254337596483 21.15579674365446 2 16.646058685307707 22.200572789660097 38.57860019266422 22.574768332367974 3 20.09465330528791 27.133641343118615 29.80629981047907 22.965405541114407 4 12.630045099516183 17.852048032189785 38.210372850466165 31.30753401782787 5 11.2308468601915 37.82709008464815 33.12528667783475 17.816776377325606 6 26.898110458888556 38.256130143903825 19.226402159475555 15.619357237732062 7 26.323863416828715 32.15298257802132 23.10216882421824 18.42098518093173 8 25.105958423058635 32.16151357480517 21.49023124357067 21.242296758565523 9 24.375429658105958 18.241910759652747 24.105421697117478 33.27723788512382 10-11 23.82784465728228 25.96297305113872 28.42558955894212 21.783592732636876 12-13 26.387444209002535 23.69778924765407 28.37409530309331 21.540671240250084 14-15 22.877442898686784 26.273461573260327 28.431898577179904 22.417196950872984 16-17 20.28923522100446 28.712733794262196 28.63763806920972 22.360392915523622 18-19 19.320182846422547 28.1329320576879 31.144335666796863 21.402549429092687 20-21 21.797281949225123 27.107755766833247 29.712154287000924 21.382807996940706 22-23 20.46808010873769 27.689702780837163 29.906958577228664 21.935258533196482 24-25 21.27609557317778 27.215103919400065 30.522089352053577 20.986711155368578 26-27 21.131040398317083 25.700140447020175 32.074774743409684 21.094044411253055 28-29 20.45092254155507 27.993896080716812 30.85629907138475 20.698882306343368 30-31 21.634385366584326 27.72557773576438 29.956921494737475 20.68311540291382 32-33 20.68999553719764 27.61766887154226 30.670374892614415 21.021960698645685 34-35 20.910245619404698 26.056450378758893 31.405673731731575 21.627630270104827 36-37 22.09719132959854 27.211211417275983 30.78527061810799 19.906326635017486 38-39 21.566958646657532 27.73381402999916 30.688047797256502 20.011179526086803 40-41 20.654793105427277 27.950681996441755 30.369693723337093 21.02483117479387 42-43 20.659568469282867 28.007587613910808 29.190718275499165 22.142125641307164 44-45 21.73956205764582 27.765353211958544 29.041980537850655 21.45310419254498 46-47 21.88526684815126 29.354004367259932 28.377491778870358 20.38323700571845 48-49 21.749030316408167 28.11082032611361 29.348675610568797 20.791473746909425 50-51 20.745088842871663 28.10865888522443 30.07482411035667 21.07142816154724 52-53 21.21762190239698 29.001027927729066 28.346073082715854 21.435277087158095 54-55 21.134894186948188 29.123732735729995 28.096111051389883 21.645262025931935 56-57 21.570874051228177 28.160597772393466 28.19468383645346 22.0738443399249 58-59 22.123471638982966 27.35288503067912 28.52199680718349 22.001646523154424 60-61 21.94986596759004 28.47887896757646 29.038327740758575 20.53292732407493 62-63 21.303908734155687 28.589006982909176 28.8009563836485 21.306127899286633 64-65 20.746987859581438 29.517540838041 28.651158162249878 21.08431314012768 66-67 21.372823514263352 27.93828324739985 27.637980016901626 23.050913221435177 68-69 21.63208366791599 28.870851924269488 27.65235258629363 21.844711821520892 70-71 22.610272950442443 28.151971449021822 27.642482319181667 21.595273281354068 72-73 21.837878926410397 27.25510013974767 29.67560789090424 21.231413042937692 74-75 21.046906327742533 28.82694586107023 29.01971578391253 21.106432027274707 76-77 21.198092423212422 28.201753865823104 28.19496349842789 22.405190212536585 78-79 21.77655521410315 28.618318995775432 28.111508926750872 21.49361686337054 80-81 21.90840310834328 28.182473047979776 28.065965645714208 21.843158197962737 82-83 21.08192932126548 27.80663099460328 29.032493151292826 22.07894653283841 84-85 20.84232021955674 28.3274942482653 28.469441781427197 22.360743750750768 86-87 21.515607512907035 28.44482640753196 27.574984750274627 22.46458132928638 88-89 21.062227691921606 28.040123994021414 27.67103405244918 23.226614261607796 90-91 21.149163522375886 26.426484039576177 28.185150939346908 24.239201498701032 92-93 21.490441649320942 27.550911496837983 28.163134846750015 22.795512007091055 94-95 20.724469772220257 27.651894447367813 28.913794577609096 22.709841202802835 96-97 19.614745302670062 27.99346591506837 29.626470695126407 22.76531808713516 98-99 20.16790841720752 28.61692561681219 28.226200270124018 22.988965695856265 100-101 19.656231380076456 28.28386351398784 28.011633656501083 24.048271449434626 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 249.0 1 195.5 2 498.0 3 755.5 4 525.5 5 435.5 6 605.0 7 1012.0 8 1456.0 9 1748.5 10 1821.5 11 1900.5 12 2102.5 13 2356.0 14 2662.5 15 2912.5 16 3295.0 17 3823.0 18 4263.0 19 4782.0 20 5388.5 21 6124.0 22 7384.0 23 9144.5 24 11259.5 25 14074.0 26 17711.0 27 22096.5 28 27742.0 29 33917.5 30 39863.0 31 45718.5 32 51977.5 33 57525.0 34 62115.5 35 66730.0 36 70414.5 37 74871.5 38 78821.5 39 80472.0 40 81082.0 41 80714.0 42 80808.5 43 82332.5 44 83817.0 45 87249.5 46 94448.5 47 115774.5 48 161493.0 49 164806.0 50 128723.5 51 105307.5 52 83984.5 53 70549.5 54 62438.5 55 56112.5 56 50195.0 57 44010.0 58 37759.5 59 31733.0 60 26108.0 61 20724.0 62 15396.5 63 10668.5 64 6956.5 65 4323.5 66 2557.5 67 1397.0 68 772.0 69 440.5 70 257.0 71 167.5 72 109.0 73 53.0 74 31.5 75 18.0 76 10.5 77 8.0 78 10.0 79 9.5 80 6.5 81 4.0 82 2.5 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05260143756859706 2 0.0047054376879545005 3 0.04181336091816479 4 0.0 5 0.0 6 0.0011094121378104106 7 0.0 8 0.0 9 0.0 10-11 3.443003186308171E-4 12-13 0.0 14-15 0.002620507980690108 16-17 0.0 18-19 0.0 20-21 0.012337428084270945 22-23 0.0 24-25 0.0 26-27 0.007651118191795936 28-29 0.010195114990568084 30-31 0.0107306932639938 32-33 0.006446067076588076 34-35 0.007536351418918997 36-37 0.02119359739127474 38-39 0.011113249173583596 40-41 0.01327469006276595 42-43 0.0 44-45 0.0 46-47 3.8255590958979674E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 9.755175694539818E-4 58-59 1.530223638359187E-4 60-61 0.010080348217691146 62-63 0.015359619770030341 64-65 0.04732216601625786 66-67 0.024426194827308522 68-69 0.0016832460021951059 70-71 2.2953354575387807E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 1.1476677287693903E-4 86-87 5.929616598641849E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.563897739744918E-5 96-97 4.5906709150775614E-4 98-99 4.78194886987246E-4 100-101 0.0011285399332899005 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2613997.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.774002494479795 #Duplication Level Percentage of deduplicated Percentage of total 1 79.22068447988437 46.5611670723512 2 13.734549667904384 16.144689128839378 3 3.2681181493342093 5.762411527836705 4 1.2989575573284784 3.053797388585895 5 0.6553260050391394 1.9258066127433928 6 0.3797956180619981 1.3393265162021022 7 0.26076592471258153 1.072837997667282 8 0.17675050400565187 0.8310667650662996 9 0.13875835674882958 0.7339845605117066 >10 0.768750173225919 8.248543621359488 >50 0.05994746740293294 2.4131506422856543 >100 0.03153644071957536 3.4112615018080916 >500 0.0029320914348175344 1.174261725756356 >1k 0.0023456731478443296 2.8976075145076887 >5k 5.864182869610824E-4 2.297092296768803 >10k+ 1.954727623203608E-4 2.1329951277099974 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 30687 1.1739493197582094 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14871 0.5688988931509869 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10140 0.3879116923240539 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9875 0.3777739607199243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8119 0.310597142995956 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7995 0.30585344971704254 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6999 0.26775088112189876 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6278 0.2401686000404744 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5337 0.20417008894807456 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5285 0.2021807982182076 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5048 0.1931142231609294 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5047 0.19307596756997042 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4928 0.18852355224585185 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 4612 0.17643478550281427 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4164 0.15929628075319138 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3784 0.1447591561887791 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 3339 0.12773541821203316 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3206 0.12264742461448885 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3151 0.12054336711174497 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2871 0.10983180164323066 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2708 0.10359614031691697 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 3.825559095897968E-5 0.0 0.0 0.0 5 0.0 3.825559095897968E-5 0.0 0.0 0.0 6 0.0 3.825559095897968E-5 0.0 0.0 0.0 7 0.0 3.825559095897968E-5 0.0 0.0 0.0 8 0.0 3.825559095897968E-5 0.0 0.0 0.0 9 0.0 3.825559095897968E-5 0.0 0.0 0.0 10-11 0.0 3.825559095897968E-5 0.0 1.1476677287693905E-4 0.0 12-13 0.0 3.825559095897968E-5 0.0 2.2953354575387804E-4 0.0 14-15 0.0 3.825559095897968E-5 0.0 3.63428114110307E-4 0.0 16-17 0.0 3.825559095897968E-5 0.0 4.7819488698724595E-4 0.0 18-19 0.0 3.825559095897968E-5 0.0 5.547060689052054E-4 0.0 20-21 0.0 3.825559095897968E-5 0.0 7.651118191795936E-4 0.0 22-23 0.0 3.825559095897968E-5 0.0 0.001511095842879697 0.0 24-25 0.0 1.1476677287693905E-4 0.0 0.0027161469580875572 0.0 26-27 0.0 1.5302236383591872E-4 0.0 0.004992354620146848 0.0 28-29 0.0 1.912779547948984E-4 0.0 0.010329009558924513 0.0 30-31 0.0 1.912779547948984E-4 0.0 0.02012244084442331 0.0 32-33 0.0 1.912779547948984E-4 0.0 0.03932674750583111 0.0 34-35 0.0 1.912779547948984E-4 0.0 0.06111330655697003 0.0 36-37 0.0 2.2953354575387804E-4 0.0 0.08703146943167876 0.0 38-39 3.825559095897968E-5 2.677891367128577E-4 0.0 0.12729547891600487 0.0 40-41 3.825559095897968E-5 3.0604472767183744E-4 0.0 0.1746941561141807 0.0 42-43 3.825559095897968E-5 3.0604472767183744E-4 0.0 0.2191088972175561 0.0 44-45 3.825559095897968E-5 3.2517252315132725E-4 0.0 0.24672943388993945 0.0 46-47 3.825559095897968E-5 3.443003186308171E-4 0.0 0.283263523255765 0.0 48-49 3.825559095897968E-5 3.443003186308171E-4 0.0 0.3113431270196561 0.0 50-51 3.825559095897968E-5 3.825559095897968E-4 0.0 0.33988179787505496 0.0 52-53 3.825559095897968E-5 3.825559095897968E-4 0.0 0.4115153919457444 0.0 54-55 3.825559095897968E-5 3.825559095897968E-4 0.0 0.5188223245856823 0.0 56-57 3.825559095897968E-5 3.825559095897968E-4 0.0 0.6858653625080671 0.0 58-59 3.825559095897968E-5 3.825559095897968E-4 0.0 0.9133713619411192 0.0 60-61 3.825559095897968E-5 3.825559095897968E-4 0.0 1.1667763964534008 0.0 62-63 3.825559095897968E-5 3.825559095897968E-4 0.0 1.2805485239654062 0.0 64-65 3.825559095897968E-5 3.825559095897968E-4 0.0 1.4466542999092962 0.0 66-67 3.825559095897968E-5 4.2081150054877647E-4 0.0 1.9183074808425564 0.0 68-69 3.825559095897968E-5 4.2081150054877647E-4 0.0 2.4211580962028645 0.0 70-71 3.825559095897968E-5 4.2081150054877647E-4 0.0 2.769857807793964 0.0 72-73 3.825559095897968E-5 5.738338643846952E-4 0.0 3.184108474493276 0.0 74-75 3.825559095897968E-5 5.738338643846952E-4 0.0 3.4964462468778654 0.0 76-77 3.825559095897968E-5 5.738338643846952E-4 0.0 3.6979767000497707 0.0 78-79 3.825559095897968E-5 5.738338643846952E-4 0.0 3.858879715623239 0.0 80-81 3.825559095897968E-5 5.738338643846952E-4 0.0 4.030589935642619 0.0 82-83 3.825559095897968E-5 5.738338643846952E-4 0.0 4.333842005174452 0.0 84-85 3.825559095897968E-5 5.738338643846952E-4 0.0 4.998896326200834 0.0 86-87 3.825559095897968E-5 5.92961659864185E-4 0.0 6.061674898632248 0.0 88-89 3.825559095897968E-5 6.120894553436749E-4 0.0 7.3005057006568865 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4400 0.0 57.237595 2 TATAACG 695 0.0 55.380695 2 ATAACGC 770 0.0 51.220695 3 TAACGCA 800 0.0 49.866226 4 GTACCTG 5300 0.0 48.62273 1 TATCACG 455 0.0 48.040176 2 ACCTGGG 5670 0.0 43.160088 3 TCACGCA 495 0.0 42.214794 4 GTATAAC 1070 0.0 40.436497 1 CCTGGGG 3175 0.0 39.93785 4 GTACCCG 315 0.0 33.206875 1 GCGATCG 65 0.005834604 29.241848 2 GTACACG 350 0.0 28.527725 1 CTGGGGG 2185 0.0 26.082367 5 TACACGG 355 0.0 25.432169 2 CACGCAG 845 0.0 24.729374 5 ATCACGC 880 0.0 24.298979 3 GTACCGG 220 2.1482265E-9 23.773106 1 TCGACTC 540 0.0 22.866346 4 ACAACGC 295 5.456968E-12 22.550915 3 >>END_MODULE