##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003864_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1767600 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.674059742023083 31.0 31.0 34.0 27.0 34.0 2 30.59393131930301 31.0 31.0 34.0 27.0 34.0 3 30.663140416383797 31.0 31.0 34.0 27.0 34.0 4 34.17724824620955 37.0 35.0 37.0 30.0 37.0 5 34.15016123557366 37.0 35.0 37.0 30.0 37.0 6 34.25047522063815 37.0 35.0 37.0 30.0 37.0 7 34.258616202760805 37.0 35.0 37.0 30.0 37.0 8 34.235599117447386 37.0 35.0 37.0 30.0 37.0 9 35.66755940257977 39.0 35.0 39.0 30.0 39.0 10-11 35.51068426114506 38.0 35.0 39.0 30.0 39.0 12-13 35.37845779588142 38.0 35.0 39.0 29.5 39.0 14-15 36.31880233084408 39.0 36.0 41.0 29.0 41.0 16-17 36.37926114505544 39.0 36.0 41.0 29.5 41.0 18-19 36.33566728897941 39.0 36.0 41.0 29.0 41.0 20-21 36.22134221543335 39.0 36.0 41.0 28.0 41.0 22-23 36.119241344195515 39.0 35.5 41.0 27.0 41.0 24-25 35.91578779135551 39.0 35.0 41.0 27.0 41.0 26-27 35.732382609187596 39.0 35.0 40.0 27.0 41.0 28-29 35.55754667345553 38.0 35.0 40.0 27.0 41.0 30-31 35.31733452138492 38.0 34.0 40.0 26.0 41.0 32-33 35.03883118352569 38.0 34.0 40.0 25.0 41.0 34-35 35.00172126046617 38.0 34.0 40.0 25.0 41.0 36-37 34.84651787734782 38.0 34.0 40.0 25.0 41.0 38-39 34.836733423851555 38.0 34.0 40.0 24.5 41.0 40-41 34.95466395112016 38.0 34.0 40.0 25.0 41.0 42-43 34.80975107490382 38.0 34.0 40.0 24.0 41.0 44-45 34.586819981896355 38.0 34.0 40.0 23.5 41.0 46-47 34.31077591083955 38.0 33.0 40.0 22.5 41.0 48-49 34.03170287395338 38.0 33.0 40.0 20.5 41.0 50-51 33.235309742023084 37.5 32.0 39.5 18.5 40.5 52-53 33.400018386512784 37.5 33.0 39.5 19.0 40.5 54-55 33.73807111337406 38.0 33.0 40.0 19.0 41.0 56-57 33.54214047295768 38.0 33.0 40.0 17.5 41.0 58-59 33.263884080108625 38.0 32.5 40.0 14.5 41.0 60-61 32.943739250961755 37.0 32.0 40.0 13.0 41.0 62-63 32.71657982575243 37.0 32.0 40.0 13.0 41.0 64-65 32.422955136908804 36.5 31.0 39.5 9.5 41.0 66-67 32.03534510070152 36.0 31.0 39.0 8.0 41.0 68-69 31.639038243946594 35.5 31.0 39.0 7.5 40.5 70-71 31.184906653088934 35.0 30.0 38.5 6.5 40.0 72-73 30.787451063589046 35.0 30.0 37.5 2.0 40.0 74-75 30.420182733650147 35.0 29.5 37.0 2.0 39.0 76-77 29.994430866711923 34.5 29.0 36.5 2.0 39.0 78-79 29.427004695632498 34.0 29.0 36.0 2.0 39.0 80-81 28.97329995474089 34.0 28.0 36.0 2.0 37.5 82-83 28.606796786603304 34.0 27.5 35.0 2.0 37.0 84-85 28.353208870785245 34.0 27.0 35.0 2.0 37.0 86-87 28.140088538130797 34.0 27.0 35.0 2.0 36.0 88-89 27.79810675492193 34.0 26.0 35.0 2.0 36.0 90-91 27.433708701063587 34.0 26.0 35.0 2.0 35.5 92-93 27.170493041412083 33.5 25.0 35.0 2.0 35.0 94-95 26.910435618918306 33.0 25.0 35.0 2.0 35.0 96-97 26.583186524100476 33.0 25.0 35.0 2.0 35.0 98-99 26.30861026250283 33.0 24.5 35.0 2.0 35.0 100-101 25.99209238515501 33.0 23.5 35.0 2.0 35.0 102-103 25.653900203665987 32.0 21.5 35.0 2.0 35.0 104-105 25.302451063589046 32.0 19.0 35.0 2.0 35.0 106-107 24.920132948630915 32.0 18.0 35.0 2.0 35.0 108-109 24.594177415704912 32.0 16.5 34.0 2.0 35.0 110-111 24.207053914912876 31.5 8.5 34.0 2.0 35.0 112-113 23.69580730934601 31.0 3.5 34.0 2.0 35.0 114-115 23.18331042090971 31.0 2.0 34.0 2.0 35.0 116-117 22.504080957230144 31.0 2.0 34.0 2.0 35.0 118-119 21.707505091649693 30.5 2.0 34.0 2.0 35.0 120-121 20.091708248472507 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5331740371679992 1101 2 0.3173721107675078 1101 3 0.49136560260550155 1101 4 0.732516211741391 1101 5 0.6804724794302857 1101 6 0.600273629887937 1101 7 0.48322002335547154 1101 8 0.6102921907460654 1101 9 0.6692587815451745 1101 10-11 0.734012911886019 1101 12-13 0.7520047844643898 1101 14-15 0.9612110490432215 1101 16-17 0.7277510600358283 1101 18-19 0.7494041359947161 1101 20-21 0.7751775994829728 1101 22-23 0.7289998424007962 1101 24-25 0.7426285499930927 1101 26-27 0.7572748419284707 1101 28-29 0.8449788471471962 1101 30-31 0.7371968881612645 1101 32-33 0.6112179869578043 1101 34-35 0.8048776023758251 1101 36-37 0.8074146500616735 1101 38-39 0.7482414328030416 1101 40-41 0.7105020536736077 1101 42-43 0.7645515580926912 1101 44-45 0.8176030555680001 1101 46-47 0.8973362214088851 1101 48-49 0.9373087614624183 1101 50-51 0.7252965191909766 1101 52-53 0.5634971305602008 1101 54-55 0.765916420792685 1101 56-57 0.7638908676828464 1101 58-59 0.8727792688328719 1101 60-61 0.6608887168511046 1101 62-63 0.6778987561359457 1101 64-65 0.8014425012333319 1101 66-67 0.5606952344046157 1101 68-69 0.6788469802242183 1101 70-71 0.3709903173877578 1101 72-73 0.28312492718495363 1101 74-75 0.3258318696968381 1101 76-77 0.12726309731431584 1101 78-79 0.6098742380895921 1101 80-81 0.6190690598446018 1101 82-83 0.6212688574098131 1101 84-85 0.5599866847648443 1101 86-87 0.4828593514481945 1101 88-89 0.3557147845020907 1101 90-91 0.6445448513272538 1101 92-93 0.5352262672317707 1101 94-95 0.4346829864699693 1101 96-97 0.6041416830291979 1101 98-99 0.5194108134805973 1101 100-101 0.424968919110686 1101 102-103 0.28850344170698605 1101 104-105 0.36785076676211403 1101 106-107 0.09109156813878982 1101 108-109 0.1419396570326299 1101 110-111 -0.0375312324237953 1101 112-113 -0.07135577639926183 1101 114-115 0.0947349933084034 1101 116-117 0.02093805882416433 1101 118-119 -0.015344137045595119 1101 120-121 0.17772315056263466 1102 1 0.649177980350327 1102 2 0.6798957474570244 1102 3 0.6265634338552211 1102 4 1.009286835033052 1102 5 0.923611790269554 1102 6 0.9682200384553923 1102 7 0.798283876023838 1102 8 0.962707389921583 1102 9 0.815344276907453 1102 10-11 1.0076213718044613 1102 12-13 0.9270827519331348 1102 14-15 1.0372528557376697 1102 16-17 0.8846856838652002 1102 18-19 0.7816114219202461 1102 20-21 0.798539610505955 1102 22-23 0.6937872242173455 1102 24-25 0.9414330488056564 1102 26-27 0.7991185037109645 1102 28-29 0.6840598168115761 1102 30-31 0.8073138393643831 1102 32-33 0.617161438138524 1102 34-35 0.7129877874365462 1102 36-37 0.715103631555607 1102 38-39 0.6249641105823116 1102 40-41 0.6877196456438526 1102 42-43 0.6521804956739032 1102 44-45 0.7277092526374958 1102 46-47 0.6782693665448818 1102 48-49 0.48564971230706533 1102 50-51 0.43408040591410213 1102 52-53 0.5497123476592733 1102 54-55 0.5548156904077715 1102 56-57 0.4745358916555986 1102 58-59 0.5182495381342989 1102 60-61 0.8243506516989854 1102 62-63 0.636362931428323 1102 64-65 0.5165289823911934 1102 66-67 0.6215569093609226 1102 68-69 0.32414764787668204 1102 70-71 0.34079539871589404 1102 72-73 0.5117497423482824 1102 74-75 0.5559394110330338 1102 76-77 0.49810841702575104 1102 78-79 0.7327442474994577 1102 80-81 0.7133906532487337 1102 82-83 0.7328806331861308 1102 84-85 0.620311101129051 1102 86-87 0.6061642311363258 1102 88-89 0.6252216626667177 1102 90-91 0.6452880067692917 1102 92-93 0.7139727385316768 1102 94-95 0.5192983021485986 1102 96-97 0.6713778706888291 1102 98-99 0.8701071436324987 1102 100-101 0.8119602261860095 1102 102-103 0.7622060002946114 1102 104-105 0.571897681670027 1102 106-107 0.5144293823611292 1102 108-109 0.7392206535823469 1102 110-111 0.7393838995383888 1102 112-113 0.5513683800733808 1102 114-115 0.46401849915596216 1102 116-117 0.6068689635156552 1102 118-119 0.5259031184098895 1102 120-121 0.6110643254517356 1103 1 0.5923844053378104 1103 2 0.7137727321320142 1103 3 0.7536068235640805 1103 4 0.7983180327475878 1103 5 0.8874510378192753 1103 6 0.8568908217285554 1103 7 0.7918116349346178 1103 8 0.7757406957294535 1103 9 0.8061206640075014 1103 10-11 0.8317642073403064 1103 12-13 0.7648641950752122 1103 14-15 0.9583378051349811 1103 16-17 0.9405597863519759 1103 18-19 0.8881490477224077 1103 20-21 1.0870785440023738 1103 22-23 0.9676768349058662 1103 24-25 0.9023337420151378 1103 26-27 0.9213502220606742 1103 28-29 1.0900984144597956 1103 30-31 0.9565079445943141 1103 32-33 1.0879203329489826 1103 34-35 1.0332792895845557 1103 36-37 0.8971590742223228 1103 38-39 0.8750092034877923 1103 40-41 0.829482698395978 1103 42-43 0.7492001129666619 1103 44-45 0.814273539620018 1103 46-47 0.6599998114897332 1103 48-49 0.5708970281048948 1103 50-51 0.5150470662865487 1103 52-53 0.6546121850429856 1103 54-55 0.6618487510229727 1103 56-57 0.620408505137064 1103 58-59 0.4819556206975335 1103 60-61 0.46718196007902435 1103 62-63 0.4663137097036838 1103 64-65 0.6044550986050012 1103 66-67 0.5496626127679782 1103 68-69 0.44945179012976766 1103 70-71 0.37862482352770144 1103 72-73 0.2502496422983995 1103 74-75 0.3649533419225186 1103 76-77 0.42366836757386395 1103 78-79 0.7172196056357691 1103 80-81 0.5987516877175629 1103 82-83 0.5199695479408071 1103 84-85 0.45914013231736917 1103 86-87 0.5874728695278613 1103 88-89 0.6767390154501243 1103 90-91 0.5893459126359701 1103 92-93 0.49615956125737526 1103 94-95 0.6533731319463669 1103 96-97 0.5822186279114447 1103 98-99 0.7100660809054453 1103 100-101 0.5928698863076285 1103 102-103 0.5884674978112123 1103 104-105 0.7190080014632763 1103 106-107 0.6499229946692395 1103 108-109 0.7177799164636518 1103 110-111 0.9884363613761664 1103 112-113 1.0150013040946533 1103 114-115 0.857036990065513 1103 116-117 0.3945321082385185 1103 118-119 0.44464769904116963 1103 120-121 0.5116045243678116 1104 1 0.5806080219341467 1104 2 0.7162641124326541 1104 3 0.6764926826338531 1104 4 0.8325423140579673 1104 5 0.8741586440773972 1104 6 0.826061818589686 1104 7 0.669643893473939 1104 8 0.7290892339836503 1104 9 0.7541297888470737 1104 10-11 0.8408260665534897 1104 12-13 0.9505778203213211 1104 14-15 1.0503212075697945 1104 16-17 0.9390907164303357 1104 18-19 0.9434513451845348 1104 20-21 1.0507726085400293 1104 22-23 0.8191133649759195 1104 24-25 0.9404319050408532 1104 26-27 0.8654711943547468 1104 28-29 0.8478153363856151 1104 30-31 0.842223834302736 1104 32-33 0.8366629076945173 1104 34-35 0.8575695162831849 1104 36-37 0.8147177857149615 1104 38-39 0.6480232759416253 1104 40-41 0.812660220210887 1104 42-43 0.5980128152792616 1104 44-45 0.6399567644596615 1104 46-47 0.817955138837803 1104 48-49 0.8571268328162489 1104 50-51 0.7440574467232963 1104 52-53 0.5921767133731777 1104 54-55 0.6781678581077415 1104 56-57 0.8185158156469754 1104 58-59 0.79560900747218 1104 60-61 0.7685117183924959 1104 62-63 0.6936452036331673 1104 64-65 0.6133655219014145 1104 66-67 0.7454253792049634 1104 68-69 0.6787192813909471 1104 70-71 0.814502091557717 1104 72-73 0.7197897936918594 1104 74-75 0.7998598396791685 1104 76-77 0.8471052458049968 1104 78-79 0.7285145549177123 1104 80-81 0.953359290602581 1104 82-83 0.9807930526821451 1104 84-85 0.827160546445068 1104 86-87 0.7326659467835306 1104 88-89 0.6970254133203966 1104 90-91 0.8376995188039871 1104 92-93 0.8418950715881586 1104 94-95 0.899731534528609 1104 96-97 0.8598676537090952 1104 98-99 0.8125029254055285 1104 100-101 0.9385458365621098 1104 102-103 0.8167480609169431 1104 104-105 0.9687145059189604 1104 106-107 0.8405750347964549 1104 108-109 0.7913682188174924 1104 110-111 0.9239365728439246 1104 112-113 0.7228187940938682 1104 114-115 0.7352082103064106 1104 116-117 0.6623401582604878 1104 118-119 0.507608124967657 1104 120-121 0.7497014136334812 1105 1 0.6166883038576856 1105 2 0.831359632038069 1105 3 0.7497349828614865 1105 4 1.117930895210769 1105 5 1.0520002606332781 1105 6 1.083408109159926 1105 7 0.9631371606461556 1105 8 0.9702566781563604 1105 9 1.1509890338325306 1105 10-11 0.9702983936488181 1105 12-13 1.2488229550727894 1105 14-15 1.3568200652951674 1105 16-17 1.1484413359982426 1105 18-19 1.154764070888298 1105 20-21 1.3069327599061609 1105 22-23 1.2197584307753573 1105 24-25 1.237846434527853 1105 26-27 1.326848690851989 1105 28-29 1.4170753230159008 1105 30-31 1.3545450348479946 1105 32-33 1.2678514427766885 1105 34-35 1.3188604379574684 1105 36-37 1.275871085485484 1105 38-39 1.119133193389004 1105 40-41 1.233608172196874 1105 42-43 1.1891916231991075 1105 44-45 1.1759221263219004 1105 46-47 1.3563825604061464 1105 48-49 1.2336884502340268 1105 50-51 1.2649265963815353 1105 52-53 1.323750897607475 1105 54-55 1.2347141716619987 1105 56-57 1.3445273815302912 1105 58-59 1.4391217187083356 1105 60-61 1.3914754144268784 1105 62-63 1.3887175186208367 1105 64-65 1.4213312777752947 1105 66-67 1.3227978756534746 1105 68-69 1.146783304177955 1105 70-71 1.0183510414500034 1105 72-73 1.1010079822541314 1105 74-75 1.1161120245711835 1105 76-77 1.0864213996152365 1105 78-79 1.1843392063995388 1105 80-81 1.2558899432202715 1105 82-83 1.193976692374921 1105 84-85 1.0385674142526256 1105 86-87 1.1453723604003265 1105 88-89 1.1768292386193302 1105 90-91 1.2352652143448353 1105 92-93 1.3018387607581197 1105 94-95 1.32073245748035 1105 96-97 1.42621423722289 1105 98-99 1.4172142118381394 1105 100-101 1.3992388147924508 1105 102-103 1.3799333166730747 1105 104-105 1.4937411709644337 1105 106-107 1.397526005486199 1105 108-109 1.354273869960199 1105 110-111 1.481393525276765 1105 112-113 1.477680451374539 1105 114-115 1.3507178782130858 1105 116-117 1.1472072639833577 1105 118-119 1.0000404593686234 1105 120-121 1.0849033522938711 1106 1 0.5049034328054312 1106 2 0.7262949360963837 1106 3 0.639601094777408 1106 4 0.9849853683273295 1106 5 1.013569807536875 1106 6 0.9810071379805763 1106 7 0.7590080034425242 1106 8 0.9730371944737257 1106 9 0.9157516508845376 1106 10-11 1.0147880255196498 1106 12-13 1.1929181824105655 1106 14-15 1.1961488812262004 1106 16-17 1.048344119365808 1106 18-19 0.9146565349269196 1106 20-21 1.0919968197999452 1106 22-23 0.9302848042824223 1106 24-25 1.1314049141978941 1106 26-27 1.0613524254401696 1106 28-29 1.0018259648211298 1106 30-31 1.0190840481622274 1106 32-33 0.9712206323968289 1106 34-35 1.0513918757218192 1106 36-37 0.9240631193144537 1106 38-39 1.0856493835497787 1106 40-41 1.0887197492557235 1106 42-43 1.0577893349619245 1106 44-45 0.9950547763058353 1106 46-47 0.9657696405185732 1106 48-49 1.1316186626191893 1106 50-51 1.0749571815127084 1106 52-53 1.0526645387514435 1106 54-55 1.2286114016733691 1106 56-57 1.125399008420196 1106 58-59 1.3212420282662691 1106 60-61 1.1844845899662317 1106 62-63 1.1963220744784593 1106 64-65 1.0428635829773967 1106 66-67 1.0918332541732632 1106 68-69 1.0907935423559643 1106 70-71 0.910371765645376 1106 72-73 1.028247940099508 1106 74-75 0.9913961899392554 1106 76-77 0.9540118527555066 1106 78-79 1.186210064548792 1106 80-81 1.1800776935955923 1106 82-83 1.3047389519241364 1106 84-85 1.3771310535871422 1106 86-87 1.3610202903385513 1106 88-89 1.2736708221154238 1106 90-91 1.2557081230138394 1106 92-93 1.2923937235219007 1106 94-95 1.2835738260361147 1106 96-97 1.5387736062633692 1106 98-99 1.7428438718485992 1106 100-101 1.3740737987432112 1106 102-103 1.2419175010129209 1106 104-105 1.3770104791307425 1106 106-107 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scores pass #Quality Count 2 23827.0 3 3925.0 4 3530.0 5 4091.0 6 4843.0 7 5186.0 8 6556.0 9 8123.0 10 10681.0 11 13013.0 12 13081.0 13 14963.0 14 17917.0 15 17998.0 16 14146.0 17 13725.0 18 14627.0 19 15877.0 20 17462.0 21 19238.0 22 21491.0 23 23870.0 24 26746.0 25 30448.0 26 34440.0 27 39663.0 28 45814.0 29 53767.0 30 62532.0 31 74526.0 32 88961.0 33 109518.0 34 138178.0 35 179242.0 36 239051.0 37 264165.0 38 91936.0 39 443.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.86407314459814 18.991732295772927 13.448533612239272 21.695660947389662 2 14.597700034408753 21.657714494074334 39.44846704149194 24.296118430024976 3 20.85769735858787 26.096994396797125 30.160200715831703 22.8851075287833 4 11.687574647594907 16.495079390248215 41.08717698338731 30.73016897876957 5 11.164051978513601 38.27925131865731 35.64866759568287 14.908029107146218 6 27.196746047366005 37.86519257592224 18.963686824173035 15.974374552538729 7 25.705533334207864 32.671407770063524 23.939454257896024 17.68360463783259 8 23.69775953847154 32.2899973652364 21.403918383920413 22.60832471237164 9 23.26855540816594 19.2144982714354 24.444504008604614 33.07244231179405 10-11 23.82383190107816 25.521752134839087 28.9956326648272 21.658783299255553 12-13 26.52505667156157 23.728432728361444 28.364351805648624 21.382158794428367 14-15 22.189846000834894 25.85970130868495 29.073729581024637 22.876723109455515 16-17 19.885514024688835 29.13097951932546 29.250450833803683 21.73305562218202 18-19 19.29352702164145 30.10335323905668 30.09111482796189 20.512004911339986 20-21 21.14305845370044 27.529934290755207 28.839127166714924 22.487880088829428 22-23 20.09281451812541 27.466982741017148 29.703954085561385 22.736248655296052 24-25 21.845513108802216 27.310391990730455 29.431173833329012 21.412921067138317 26-27 21.15338656439489 26.015935096053756 31.85231089692951 20.97836744262185 28-29 19.759556376151536 29.09520165277894 30.343713783778153 20.801528187291364 30-31 20.565721581845608 27.530511390995375 29.601183942382082 22.302583084776938 32-33 20.787756407202778 27.504925545603836 30.34561113705297 21.36170691014042 34-35 22.098782690063818 26.395969497356838 30.22155212009346 21.283695692485885 36-37 21.686458245958804 26.485880561228363 31.23397658105817 20.59368461175466 38-39 20.92787278356415 28.089502687094615 30.748812067121595 20.233812462219642 40-41 20.35704453285366 28.58481697723217 30.116841860511563 20.94129662940261 42-43 19.81488914544844 28.744616457258726 28.611505982696777 22.82898841459605 44-45 20.709743889318016 27.389078985050038 28.760566690664803 23.140610434967147 46-47 21.913197302661114 28.33763171090259 28.897390568212998 20.851780418223296 48-49 21.49212895231733 27.77983942714735 29.472651642709856 21.255379977825463 50-51 19.48401016568468 28.26691058221519 31.011417131262046 21.23766212083808 52-53 20.43088445499623 29.32387019553735 28.583784055158596 21.661461294307827 54-55 19.802182722797628 28.276630373539156 29.145440203351825 22.775746700311387 56-57 21.28436533395822 27.130864059400032 29.015229336273574 22.56954127036817 58-59 22.859623509096995 26.793670122935747 28.73019274209172 21.616513625875537 60-61 21.26525688759065 27.622018716199847 30.621958503377485 20.49076589283202 62-63 20.186768491975094 28.107168778644077 30.039987583763946 21.666075145616887 64-65 19.77592258988414 29.93657083114034 29.102392393634545 21.18511418534097 66-67 20.433550202576754 27.960869964608264 28.15317666941105 23.45240316340393 68-69 20.113133881560362 28.47845600155669 28.57195436982899 22.83645574705396 70-71 21.829050911326267 27.81412973806611 28.257307191124237 22.099512159483382 72-73 20.99394974455016 26.792651104409217 30.495896049424925 21.717503101615694 74-75 20.00349343681974 29.488003155509595 29.123076005369164 21.385427402301502 76-77 20.64255563053265 27.838073446347032 28.51004141247718 23.00932951064313 78-79 21.116245097300173 27.990761109169448 28.843265512176515 22.049728281353865 80-81 22.073349342753147 27.277429459921994 28.61748530248435 22.031735894840505 82-83 20.907193655125948 26.83375824727993 30.292120011305663 21.96692808628846 84-85 20.85019151041371 28.000960049223927 29.5090992061329 21.639749234229466 86-87 21.19363428528382 28.586763851524488 28.434760326937408 21.78484153625428 88-89 20.23546190115839 28.12856325952 27.779328158296323 23.856646681025286 90-91 20.678828254716418 26.357334024345626 28.843751123703566 24.12008659723439 92-93 21.710965597610404 27.261220068688356 29.27321941581795 21.754594917883285 94-95 20.73536645632392 27.166741422227396 29.638867869831735 22.459024251616945 96-97 19.178228778286535 27.827112497809896 30.513730429636293 22.480928294267276 98-99 20.42609102474846 28.52459503055557 28.612540957939807 22.43677298675616 100-101 19.54460362025794 27.105240700259415 29.386075698274354 23.964079981208293 102-103 20.899831049124728 26.471671694864618 29.154626431952117 23.473870824058537 104-105 22.060438470929565 26.41835710778433 28.991715438124277 22.529488983161826 106-107 20.81502083373753 26.907676470487534 30.202328630953534 22.074974064821397 108-109 20.825656034570812 26.887902212945107 29.394405924012002 22.892035828472075 110-111 20.40819820117981 27.935750552644762 28.328393672517038 23.32765757365839 112-113 21.05900362807327 26.539919506136904 27.74087881265132 24.660198053138505 114-115 20.354169419095154 26.735399621227796 28.473745470441987 24.436685489235067 116-117 22.06329584690398 26.666360313268235 27.43525977293505 23.83508406689274 118-119 22.064692456390258 25.911088899969094 28.71708488084328 23.30713376279737 120-121 21.284387543683085 28.182541147774735 27.7656204032355 22.76745090530669 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4182.0 1 4459.0 2 3163.5 3 1489.5 4 1231.5 5 1101.5 6 1165.5 7 1352.0 8 1628.0 9 1740.5 10 1621.5 11 1529.5 12 1578.5 13 1705.0 14 1908.0 15 2017.0 16 2175.0 17 2437.0 18 2648.5 19 2960.5 20 3323.5 21 3728.0 22 4241.0 23 5107.5 24 6226.5 25 7564.5 26 9574.0 27 11957.5 28 14982.0 29 18962.0 30 23236.0 31 27470.5 32 31504.5 33 35238.0 34 39174.5 35 43034.0 36 46967.0 37 51556.5 38 55814.5 39 58133.0 40 59942.5 41 61810.0 42 63264.5 43 65947.0 44 67171.5 45 67910.5 46 72291.0 47 90316.0 48 129156.0 49 126070.0 50 85623.5 51 64127.5 52 50692.0 53 42017.5 54 35778.0 55 30530.5 56 25708.0 57 21057.0 58 17049.0 59 13999.0 60 11539.0 61 8823.0 62 6389.5 63 4520.0 64 2965.5 65 1909.0 66 1195.0 67 685.5 68 399.5 69 260.5 70 170.0 71 115.0 72 79.0 73 51.5 74 46.5 75 32.0 76 25.0 77 23.0 78 15.0 79 16.0 80 17.0 81 11.5 82 7.0 83 6.0 84 7.5 85 8.0 86 6.5 87 4.5 88 2.0 89 2.0 90 1.5 91 1.0 92 1.0 93 0.5 94 1.5 95 1.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4721090744512333 2 0.8564720525005657 3 0.8301651957456438 4 0.8125141434713736 5 0.8198121747001584 6 0.8290902919212492 7 0.8079316587463227 8 0.7989364109527043 9 0.7989364109527043 10-11 0.7927981443765558 12-13 0.7962774383344648 14-15 0.7967866033039148 16-17 0.818030097307083 18-19 0.8441672324055216 20-21 0.8374349400316814 22-23 0.8161348721430187 24-25 0.8107886399637927 26-27 0.8125707173568681 28-29 0.8301369088028966 30-31 0.8379723919438787 32-33 0.8343799502149807 34-35 0.8466281964245305 36-37 0.853530210454854 38-39 0.8412819642453044 40-41 0.8495983254129893 42-43 0.8441106585200272 44-45 0.8308440823715773 46-47 0.8267141887304821 48-49 0.8309855170853133 50-51 0.8841083955646074 52-53 0.8352285584973976 54-55 0.8470525005657389 56-57 0.8948008599230595 58-59 0.8588198687485856 60-61 0.8758486082824168 62-63 0.8517764200045259 64-65 0.8405465037338765 66-67 0.844704684317719 68-69 0.8578298257524326 70-71 0.8821565965150487 72-73 0.8876725503507581 74-75 0.8906709662819642 76-77 0.9025797691785471 78-79 0.9348268839103869 80-81 0.9597193935279476 82-83 0.9201742475673229 84-85 0.8829203439692238 86-87 0.8677302557139623 88-89 0.8679565512559403 90-91 0.8814777098891151 92-93 0.8677302557139623 94-95 0.8611676849966057 96-97 0.8710681149581354 98-99 0.9026646300067889 100-101 0.8918024439918534 102-103 0.8664573432903371 104-105 0.8555951572754017 106-107 0.8573206607829826 108-109 0.8564720525005657 110-111 0.8552840009051821 112-113 0.8651561439239647 114-115 0.8689465942520932 116-117 0.8944614166100928 118-119 0.8897092102285585 120-121 0.8683242815116543 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1767600.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.13317737983562 #Duplication Level Percentage of deduplicated Percentage of total 1 82.80302492341852 49.79208985306876 2 11.142875029128191 13.401129612958131 3 2.608661300925377 4.706012781973754 4 1.0618149646201256 2.5540123044826357 5 0.566253488844921 1.7025310743331203 6 0.34694175508472924 1.2517626059388907 7 0.25171168024014023 1.059535618151977 8 0.188305091848188 0.9058706791706662 9 0.1381005528754785 0.7473982538083065 >10 0.8075008768553829 8.925716973085203 >50 0.05508613083703706 2.2810805876237534 >100 0.024311575319202815 2.6413224617239255 >500 0.0020890852641901416 0.9073430860124995 >1k 0.002658835790787453 4.011609890794345 >5k 2.848752632986557E-4 1.0744221212341842 >10k+ 3.7983368439820753E-4 4.038162095639895 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26272 1.4863091197103417 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 20932 1.184204571169948 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13302 0.7525458248472505 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10213 0.5777890925548766 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6965 0.39403711246888434 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6554 0.37078524553066305 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5297 0.29967187146413216 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4696 0.26567096628196424 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4637 0.26233310703779134 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4392 0.2484725050916497 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4309 0.24377687259560984 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3799 0.21492419099343746 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3677 0.20802217696311381 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3303 0.18686354378818737 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2922 0.16530889341479973 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2854 0.16146186920117672 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2615 0.14794071056800182 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2592 0.14663951120162932 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2491 0.14092554876668928 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2321 0.13130798823263182 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2276 0.1287621633853813 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2134 0.12072867164516858 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2065 0.11682507354605115 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2049 0.11591989137813986 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2026 0.11461869201176737 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2021 0.11433582258429509 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1914 0.10828241683638831 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1850 0.10466168816474315 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.657388549445576E-5 0.0 10-11 0.0 0.0 0.0 5.657388549445576E-5 0.0 12-13 0.0 0.0 0.0 8.486082824168364E-5 0.0 14-15 0.0 0.0 0.0 1.6972165648336727E-4 0.0 16-17 0.0 0.0 0.0 2.2629554197782303E-4 0.0 18-19 0.0 0.0 0.0 2.5458248472505095E-4 0.0 20-21 0.0 0.0 0.0 7.354605114279248E-4 0.0 22-23 0.0 0.0 0.0 0.0017537904503281286 0.0 24-25 0.0 0.0 0.0 0.0036773025571396243 0.0 26-27 0.0 0.0 0.0 0.005855397148676171 0.0 28-29 0.0 0.0 0.0 0.011823942068341253 0.0 30-31 0.0 0.0 0.0 0.02642000452591084 0.0 32-33 0.0 0.0 0.0 0.049304141208418195 0.0 34-35 0.0 0.0 0.0 0.06729463679565512 0.0 36-37 0.0 2.828694274722788E-5 0.0 0.08921701742475674 0.0 38-39 0.0 5.657388549445576E-5 0.0 0.12516972165648338 0.0 40-41 0.0 5.657388549445576E-5 0.0 0.22660669834804253 0.0 42-43 0.0 1.1314777098891151E-4 0.0 0.29673002941842047 0.0 44-45 0.0 1.1314777098891151E-4 0.0 0.36623104774835935 0.0 46-47 0.0 1.1314777098891151E-4 0.0 0.5433073093460059 0.0 48-49 0.0 1.1314777098891151E-4 0.0 0.6286490156143925 0.0 50-51 0.0 1.1314777098891151E-4 0.0 0.7299162706494682 0.0 52-53 0.0 1.6972165648336727E-4 0.0 0.8338142113600362 0.0 54-55 0.0 1.6972165648336727E-4 0.0 0.9560703779135551 0.0 56-57 0.0 1.6972165648336727E-4 0.0 1.406653088934148 0.0 58-59 0.0 1.6972165648336727E-4 0.0 1.7260975333785924 0.0 60-61 0.0 1.6972165648336727E-4 0.0 1.8950554424077846 0.0 62-63 0.0 1.6972165648336727E-4 0.0 2.1023138719167234 0.0 64-65 0.0 1.6972165648336727E-4 0.0 2.310590631364562 0.0 66-67 0.0 1.9800859923059516E-4 0.0 2.7557705363204343 0.0 68-69 0.0 2.2629554197782303E-4 0.0 3.2108791581805836 0.0 70-71 0.0 2.2629554197782303E-4 0.0 3.3523138719167234 0.0 72-73 0.0 2.2629554197782303E-4 0.0 3.4758712378366146 0.0 74-75 0.0 2.2629554197782303E-4 0.0 3.8426114505544238 0.0 76-77 0.0 2.2629554197782303E-4 0.0 4.201544467073998 0.0 78-79 0.0 2.2629554197782303E-4 0.0 4.479548540393754 0.0 80-81 0.0 2.2629554197782303E-4 0.0 4.709549671871464 0.0 82-83 0.0 2.2629554197782303E-4 0.0 5.168957909029192 0.0 84-85 0.0 2.2629554197782303E-4 0.0 5.96724372029871 0.0 86-87 0.0 2.2629554197782303E-4 0.0 6.945264765784114 0.0 88-89 0.0 2.2629554197782303E-4 0.0 7.999632269744286 0.0 90-91 0.0 2.2629554197782303E-4 0.0 8.964556460737723 0.0 92-93 0.0 2.2629554197782303E-4 0.0 9.873359357320659 0.0 94-95 0.0 2.2629554197782303E-4 0.0 10.75387531115637 0.0 96-97 0.0 2.2629554197782303E-4 0.0 11.80165195745644 0.0 98-99 0.0 2.2629554197782303E-4 0.0 12.85785811269518 0.0 100-101 0.0 2.5458248472505095E-4 0.0 13.583587915818057 0.0 102-103 0.0 2.828694274722788E-4 0.0 14.397290110884816 0.0 104-105 0.0 2.828694274722788E-4 0.0 15.338622991627066 0.0 106-107 0.0 2.828694274722788E-4 0.0 16.34651504865354 0.0 108-109 0.0 2.828694274722788E-4 0.0 17.376499207965605 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2240 0.0 55.45781 2 ACCTGGG 2475 0.0 48.038998 3 TATAACG 415 0.0 45.732277 2 TAACGCA 475 0.0 43.53315 4 ATAACGC 520 0.0 41.973873 3 GTACCTG 3145 0.0 39.958748 1 GTATAAC 650 0.0 34.588142 1 CCTGGGG 1780 0.0 31.30134 4 GTACACG 230 3.8380676E-10 27.570257 1 AGTACGC 495 0.0 24.398947 2 TATCACG 360 0.0 23.963253 2 TCACGCA 345 3.6379788E-12 23.308813 4 GTACGCA 495 0.0 23.207245 3 GAGTACG 600 0.0 23.058764 1 GTCTCGC 300 3.3287506E-10 23.058764 1 GTACCGG 180 2.7811422E-5 22.418242 1 AGTACTT 5995 0.0 21.711128 12-13 GTACATG 6210 0.0 21.257875 1 GAGTACT 5850 0.0 20.726692 12-13 GTACCCG 225 6.6776556E-6 20.496677 1 >>END_MODULE