##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003864_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1767600 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.56172550350758 31.0 31.0 34.0 30.0 34.0 2 31.776491287621635 31.0 31.0 34.0 30.0 34.0 3 31.809643584521385 31.0 31.0 34.0 30.0 34.0 4 35.56871577279927 37.0 35.0 37.0 33.0 37.0 5 35.430165761484496 37.0 35.0 37.0 33.0 37.0 6 35.46879271328355 37.0 35.0 37.0 33.0 37.0 7 35.39923398959041 37.0 35.0 37.0 33.0 37.0 8 35.398846458474765 37.0 35.0 37.0 33.0 37.0 9 36.94562457569586 39.0 37.0 39.0 33.0 39.0 10-11 36.86191813758769 39.0 37.0 39.0 33.0 39.0 12-13 36.80697159990948 39.0 37.0 39.0 32.0 39.0 14-15 37.85333587915818 40.0 37.0 41.0 32.5 41.0 16-17 37.889949083503055 40.0 37.0 41.0 33.0 41.0 18-19 37.91408039149128 40.0 37.0 41.0 33.0 41.0 20-21 37.83662847929396 40.0 37.0 41.0 32.5 41.0 22-23 37.7436294976239 39.0 37.0 41.0 32.0 41.0 24-25 37.619082937316136 39.0 36.5 41.0 32.0 41.0 26-27 37.374682903371806 39.0 36.0 41.0 31.5 41.0 28-29 37.20260692464359 39.0 36.0 40.5 31.0 41.0 30-31 36.977985969676396 39.0 36.0 40.0 31.0 41.0 32-33 36.70876018329939 39.0 35.0 40.0 30.0 41.0 34-35 36.64459097080787 39.0 35.0 40.0 30.0 41.0 36-37 36.42669014482915 38.5 35.0 40.0 30.0 41.0 38-39 36.16931998189636 38.0 35.0 40.0 30.0 41.0 40-41 36.34028117221091 39.0 35.0 40.0 30.0 41.0 42-43 36.20319755600815 39.0 35.0 40.0 30.0 41.0 44-45 36.0392215433356 39.0 35.0 40.0 29.5 41.0 46-47 35.75874349400317 39.0 35.0 40.0 28.5 41.0 48-49 35.484172041185786 38.5 35.0 40.0 27.0 41.0 50-51 35.22887927132835 38.0 34.0 40.0 27.0 41.0 52-53 35.06871464132156 38.0 34.0 40.0 26.0 41.0 54-55 34.855293335596286 38.0 34.0 40.0 26.0 41.0 56-57 34.58698036886173 38.0 34.0 40.0 25.5 41.0 58-59 34.29756817153202 38.0 33.0 40.0 24.0 41.0 60-61 33.81234894772573 37.0 33.0 40.0 23.0 41.0 62-63 33.6105003960172 37.0 32.5 40.0 22.5 41.0 64-65 33.1603439692238 36.5 32.0 39.0 22.0 41.0 66-67 32.77900175379045 36.0 31.5 39.0 21.0 40.5 68-69 32.481016915591766 35.5 31.0 39.0 20.5 40.0 70-71 32.034933242815114 35.0 31.0 38.0 19.5 40.0 72-73 31.62935534057479 35.0 31.0 37.5 18.0 40.0 74-75 31.264647827562797 35.0 30.5 37.0 18.0 39.0 76-77 30.28692577506223 34.0 29.5 36.0 15.5 39.0 78-79 30.648109866485633 34.0 30.0 36.0 14.5 39.0 80-81 30.424558157954287 34.0 30.0 36.0 11.5 37.5 82-83 30.109831692690655 34.0 30.0 35.0 8.5 37.0 84-85 29.769121690427696 34.0 30.0 35.0 7.0 37.0 86-87 29.447357433808556 34.0 29.0 35.0 3.5 36.0 88-89 29.117744116315908 34.0 29.0 35.0 2.0 36.0 90-91 28.80938758768952 34.0 29.0 35.0 2.0 35.5 92-93 28.55562344421815 34.0 29.0 35.0 2.0 35.0 94-95 28.195979293957908 34.0 29.0 35.0 2.0 35.0 96-97 27.767172154333558 34.0 28.0 35.0 2.0 35.0 98-99 27.373729633401222 34.0 27.0 35.0 2.0 35.0 100-101 26.36895790902919 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.03984575504837906 1101 2 0.03330801337036604 1101 3 0.07711709334475714 1101 4 0.13740585162870644 1101 5 0.16093960583791045 1101 6 0.1433688282696508 1101 7 0.10361179363420092 1101 8 0.08079084413842708 1101 9 0.08734057401568407 1101 10-11 0.14462999635873786 1101 12-13 0.0010710227565695618 1101 14-15 -0.015291031187892656 1101 16-17 0.023217900706221428 1101 18-19 -0.03728355120446025 1101 20-21 -0.013772482945206832 1101 22-23 -0.011346889021623952 1101 24-25 -0.024669160518776323 1101 26-27 0.0297168890385322 1101 28-29 -0.06283252813263118 1101 30-31 -0.13090635342340562 1101 32-33 0.01747163752783365 1101 34-35 -0.0371289330885034 1101 36-37 -0.2831994934588451 1101 38-39 -0.20151418499332152 1101 40-41 -0.1412981786646128 1101 42-43 -0.006897729661332619 1101 44-45 -0.14526597873393143 1101 46-47 -0.31289829339076647 1101 48-49 -0.18500498119549036 1101 50-51 -0.2027221819818621 1101 52-53 -0.1428062942199304 1101 54-55 -0.023360504405140148 1101 56-57 -0.1145084809157737 1101 58-59 -0.32550330401252836 1101 60-61 -0.18686212816943026 1101 62-63 -0.3972880570597823 1101 64-65 -0.41660209184311725 1101 66-67 -0.41803948723964623 1101 68-69 -0.46060599458278517 1101 70-71 -0.23267457844887218 1101 72-73 -0.3788777621809096 1101 74-75 -0.5611981553446768 1101 76-77 -0.554851276939921 1101 78-79 -0.47204069093540824 1101 80-81 -0.44583411368655135 1101 82-83 -0.38570799582063486 1101 84-85 -0.6191361827624107 1101 86-87 -0.6903347966164617 1101 88-89 -0.5777404231445615 1101 90-91 -0.5255380026948764 1101 92-93 -0.49413643397626217 1101 94-95 -0.46825551055912484 1101 96-97 -0.34538765813664796 1101 98-99 -0.5132158327534952 1101 100-101 -0.5101961463756481 1102 1 -0.058058085073501076 1102 2 0.038904643741830114 1102 3 0.005803744776400777 1102 4 0.012469256208177626 1102 5 -0.04526284072748865 1102 6 0.054050819576723086 1102 7 -0.04514655202157769 1102 8 -0.036788131530350654 1102 9 -0.008481893161807363 1102 10-11 0.00664438001246026 1102 12-13 0.017158086429994057 1102 14-15 0.10371219505218932 1102 16-17 -0.03164319173452412 1102 18-19 -0.06866456119684727 1102 20-21 0.03202914809839541 1102 22-23 -0.07774918323679003 1102 24-25 -0.020725754146951658 1102 26-27 -0.1606660029272362 1102 28-29 -0.008978291452073961 1102 30-31 -0.15550038487924667 1102 32-33 -1.2100485241717251E-4 1102 34-35 -0.16324512254049495 1102 36-37 -0.2628658912721633 1102 38-39 -0.04709087540869916 1102 40-41 0.10272229899815954 1102 42-43 0.07381405955764819 1102 44-45 0.07862029630303624 1102 46-47 0.06227041208040163 1102 48-49 -0.04118121456018997 1102 50-51 0.11305211563878714 1102 52-53 0.08596280556946567 1102 54-55 -0.07150339995562405 1102 56-57 0.12772748269939882 1102 58-59 -0.08349160889220997 1102 60-61 -0.11258287916643184 1102 62-63 -0.20677813189063698 1102 64-65 -0.13937172933920294 1102 66-67 -0.15349183298503988 1102 68-69 -0.11842402568015586 1102 70-71 0.014496412275686765 1102 72-73 -0.0037375103549486255 1102 74-75 0.0710454257818185 1102 76-77 0.01152211554114757 1102 78-79 0.17585934043081863 1102 80-81 0.07912130146781493 1102 82-83 -0.03632142952588069 1102 84-85 0.06993358467945043 1102 86-87 -0.07798613864272141 1102 88-89 -0.12062611566595649 1102 90-91 0.052573526632542666 1102 92-93 0.031428382083245054 1102 94-95 0.11344879557520926 1102 96-97 0.07302812355444033 1102 98-99 -0.029843515685790578 1102 100-101 -0.08194302095556338 1103 1 -0.0032946302429692764 1103 2 -0.054090079448794626 1103 3 -0.06384647519243103 1103 4 0.06787833810722788 1103 5 0.06476666694253197 1103 6 0.04729839184045659 1103 7 0.07178545821075488 1103 8 0.05089572774596718 1103 9 -0.10650564960750586 1103 10-11 0.031858007652154186 1103 12-13 -0.03739223396448921 1103 14-15 0.1295099167331344 1103 16-17 -0.07303225520184498 1103 18-19 0.013054117317985003 1103 20-21 0.09700297542450897 1103 22-23 -0.014066492618276527 1103 24-25 -0.15545595765686926 1103 26-27 0.018484705078350316 1103 28-29 -0.14331839435784133 1103 30-31 -0.09644171337260588 1103 32-33 -0.17388663353676037 1103 34-35 -0.21805190883488024 1103 36-37 -0.1550613192273289 1103 38-39 -0.01790766293374446 1103 40-41 0.07769561902908606 1103 42-43 0.09919889932282899 1103 44-45 0.27573829598174626 1103 46-47 0.28424728505650876 1103 48-49 0.25827407723318174 1103 50-51 0.33150434393289174 1103 52-53 0.33078299291170055 1103 54-55 0.13078302172505119 1103 56-57 0.26154936948051244 1103 58-59 0.2239070800315659 1103 60-61 0.4288274390297815 1103 62-63 0.20552837961886183 1103 64-65 0.16405396929552296 1103 66-67 0.032488219040899935 1103 68-69 0.06327231775492947 1103 70-71 -0.012422290852352802 1103 72-73 0.1780544206453918 1103 74-75 0.14191456335182195 1103 76-77 0.17516720525254215 1103 78-79 0.19620951032773348 1103 80-81 0.26050845318383153 1103 82-83 0.21065781552493945 1103 84-85 0.06639872363605903 1103 86-87 0.07800724867058051 1103 88-89 0.09085209553606077 1103 90-91 0.07276469706587463 1103 92-93 -0.03513039041271426 1103 94-95 -0.20584432146248588 1103 96-97 0.025215904504378273 1103 98-99 0.06092474529691572 1103 100-101 0.11607577996771212 1104 1 -0.01931829576113131 1104 2 -0.13529157484309096 1104 3 -0.08084692596195708 1104 4 -0.08384818466701205 1104 5 -0.14293028761513682 1104 6 -0.18959974769146726 1104 7 -0.08829221284637612 1104 8 -0.039776881247206575 1104 9 -0.03465355296308559 1104 10-11 -0.07982067363821699 1104 12-13 -0.07385196415852135 1104 14-15 -0.07413589116922026 1104 16-17 -0.04854693092259055 1104 18-19 -0.09427762192824218 1104 20-21 -0.07087647592458524 1104 22-23 -0.021657866620884647 1104 24-25 -0.027866244259953987 1104 26-27 0.13045045414243361 1104 28-29 -0.14005296894675467 1104 30-31 -0.08806615135546991 1104 32-33 -0.06528868245055719 1104 34-35 -0.10794136769207796 1104 36-37 -0.12888496054404186 1104 38-39 -0.10878041737144173 1104 40-41 -0.14047978103182146 1104 42-43 -0.02005959777712718 1104 44-45 0.11301993164060775 1104 46-47 -0.005751434107004627 1104 48-49 0.1578733617574528 1104 50-51 0.09660140798674632 1104 52-53 0.17170321849931725 1104 54-55 0.09544396449147285 1104 56-57 -0.11083626750684061 1104 58-59 0.025792811017680606 1104 60-61 0.04555728655362401 1104 62-63 -0.06336708407342684 1104 64-65 -0.049193984373587796 1104 66-67 0.010765656716884564 1104 68-69 -0.03686751279252576 1104 70-71 -0.044495215030369906 1104 72-73 0.13444724541351505 1104 74-75 0.0060270108888254015 1104 76-77 0.017738365390837174 1104 78-79 0.07598790910284947 1104 80-81 0.11844835388244235 1104 82-83 0.12276884399890164 1104 84-85 -0.010738887502458994 1104 86-87 -0.050410290816572 1104 88-89 0.008240382651955258 1104 90-91 -0.005158281494619388 1104 92-93 0.011348877845161809 1104 94-95 -0.06336458898983466 1104 96-97 -0.10527019771203783 1104 98-99 -0.006998981007900085 1104 100-101 0.08060616232925 1105 1 0.11375041360962612 1105 2 0.17328187215849766 1105 3 0.12166813036169799 1105 4 0.14963500647839112 1105 5 0.1503341655064574 1105 6 0.2583699380380793 1105 7 0.07918505068122528 1105 8 0.18621341929441115 1105 9 0.10808485870827411 1105 10-11 0.19069525074232985 1105 12-13 0.1098364670338654 1105 14-15 0.2670338103618306 1105 16-17 0.22421249983319314 1105 18-19 0.10789857412537884 1105 20-21 0.1900281039989835 1105 22-23 0.14327961224844898 1105 24-25 0.16675010999344408 1105 26-27 0.18908456764959425 1105 28-29 0.25961142856173325 1105 30-31 0.19624030863977993 1105 32-33 0.21404171323870003 1105 34-35 0.2874408466841132 1105 36-37 0.36296366716164385 1105 38-39 0.26165580686838297 1105 40-41 0.30722607594092466 1105 42-43 0.45233406397106535 1105 44-45 0.3915558911025627 1105 46-47 0.3154977213075014 1105 48-49 0.37832531055029506 1105 50-51 0.4188028864538538 1105 52-53 0.41775450875917386 1105 54-55 0.29984327373981756 1105 56-57 0.45077204388137915 1105 58-59 0.4545825180792633 1105 60-61 0.382638476037819 1105 62-63 0.3766624719298335 1105 64-65 0.3679339016462393 1105 66-67 0.1691394814155629 1105 68-69 0.3006692526489587 1105 70-71 0.4444020448957211 1105 72-73 0.2993907524557038 1105 74-75 0.32236025546693625 1105 76-77 0.06800896622267061 1105 78-79 0.35725398957459475 1105 80-81 0.6005902124373961 1105 82-83 0.452073947013961 1105 84-85 0.5222135422616354 1105 86-87 0.6212516790056242 1105 88-89 0.45182232809468204 1105 90-91 0.49999234330625697 1105 92-93 0.6217828242051979 1105 94-95 0.5277046857465777 1105 96-97 0.6492859466378249 1105 98-99 0.6434647265697926 1105 100-101 0.9106009139710949 1106 1 -0.05014871357911943 1106 2 0.07687839407308772 1106 3 0.024809549033651734 1106 4 0.01686329613460913 1106 5 0.08361438518424791 1106 6 -0.0012664618869138167 1106 7 0.03316808136176519 1106 8 0.12842846709121858 1106 9 0.15061562752569557 1106 10-11 -0.008965395767468465 1106 12-13 0.05468974284506345 1106 14-15 0.07809440927770339 1106 16-17 0.03060637140196576 1106 18-19 0.12464280939292394 1106 20-21 0.05637469966223563 1106 22-23 0.0884054016880782 1106 24-25 0.18159908699811922 1106 26-27 0.1286454862343689 1106 28-29 0.10082563304675318 1106 30-31 0.25900562560547513 1106 32-33 0.39852740819232224 1106 34-35 0.3792359931999485 1106 36-37 0.4619576696685499 1106 38-39 0.5427204413985436 1106 40-41 0.21677978963821687 1106 42-43 0.33807737916241365 1106 44-45 0.4990658074183969 1106 46-47 0.43531050389646 1106 48-49 0.463461145160295 1106 50-51 0.6668259289341805 1106 52-53 0.7824761665193449 1106 54-55 0.5008503130355138 1106 56-57 0.7655362510531489 1106 58-59 0.7526285350485864 1106 60-61 0.6649853502461909 1106 62-63 0.4401913205405634 1106 64-65 0.5185870570470001 1106 66-67 0.5500800866041828 1106 68-69 0.47007869313516437 1106 70-71 0.5905696917706997 1106 72-73 0.5828797713079474 1106 74-75 0.6800127342443183 1106 76-77 0.6026146329708553 1106 78-79 0.704345879854138 1106 80-81 0.5397119289771624 1106 82-83 0.5577681110551076 1106 84-85 0.49237412242505485 1106 86-87 0.6637062317378337 1106 88-89 0.7503736193267585 1106 90-91 0.8202057264121692 1106 92-93 0.7157260031945611 1106 94-95 0.8657638732940782 1106 96-97 0.9786035878651305 1106 98-99 0.8995883326104916 1106 100-101 0.9578685947320693 1107 1 -0.14839939630999766 1107 2 -0.10728303509712234 1107 3 -0.1323114167941739 1107 4 -0.0867372921333569 1107 5 -0.1593109966933497 1107 6 -0.07549601545077422 1107 7 -0.15152051087437712 1107 8 0.04355100514992216 1107 9 -0.08465957740578745 1107 10-11 0.054873658739346354 1107 12-13 -0.09913427951854459 1107 14-15 -0.0679038086361956 1107 16-17 -0.22302962292184247 1107 18-19 -0.21586006321093265 1107 20-21 -0.15959564790627923 1107 22-23 -0.26610783237737223 1107 24-25 -0.16330359123713833 1107 26-27 -0.2113751121223686 1107 28-29 -0.42885569561739345 1107 30-31 -0.29859023586987377 1107 32-33 -0.07140998873873627 1107 34-35 -0.22390125829134888 1107 36-37 -0.13682199973651876 1107 38-39 -0.2122420175211701 1107 40-41 -0.023991209491498466 1107 42-43 -0.022833072694055545 1107 44-45 -0.15350469467987438 1107 46-47 -0.1622770868140222 1107 48-49 -0.2379995931148855 1107 50-51 -0.04555653951318561 1107 52-53 -0.16073484852346098 1107 54-55 -0.2541351514038439 1107 56-57 -0.17096584946301618 1107 58-59 -0.043576087265307706 1107 60-61 -0.056060099121744145 1107 62-63 -0.22638154719846426 1107 64-65 -0.20914963187517088 1107 66-67 -0.2446648074329545 1107 68-69 -0.17147679421432116 1107 70-71 -0.006545163566666901 1107 72-73 -0.20975436630209643 1107 74-75 0.04041397856237694 1107 76-77 -0.10900514638937864 1107 78-79 -0.22133001950737707 1107 80-81 -0.13623765190154558 1107 82-83 -0.10205810749396349 1107 84-85 -0.03112447283546871 1107 86-87 -0.08818499030975602 1107 88-89 -0.3118286766240992 1107 90-91 -0.17662848964108235 1107 92-93 -0.449099146439508 1107 94-95 -0.3087053585067352 1107 96-97 0.05033367588649185 1107 98-99 0.02249742545796707 1107 100-101 0.08194886491786946 1108 1 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-0.7604492367476468 2316 90-91 -1.0052106508331171 2316 92-93 -1.0255839746877697 2316 94-95 -1.0629730658400973 2316 96-97 -0.9664241186792211 2316 98-99 -1.1904288235507572 2316 100-101 -1.4131469237210545 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 1.0 4 19.0 5 41.0 6 120.0 7 360.0 8 819.0 9 1427.0 10 2492.0 11 4133.0 12 6213.0 13 8480.0 14 9175.0 15 11331.0 16 13765.0 17 17091.0 18 15370.0 19 11432.0 20 10794.0 21 10919.0 22 11665.0 23 12800.0 24 15090.0 25 18460.0 26 22630.0 27 28088.0 28 35409.0 29 43135.0 30 53483.0 31 65436.0 32 79098.0 33 98067.0 34 125661.0 35 163944.0 36 228060.0 37 307512.0 38 279170.0 39 55869.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.02573675493877 20.20870147967945 14.012465939977382 20.75309582540439 2 15.421041911831265 23.16222730152983 38.47865936453336 22.938071422105548 3 21.521788467056535 26.835155618162894 29.922390418134142 21.720665496646426 4 12.591536546730028 17.56387191672324 39.71560307761937 30.12898845892736 5 11.84306404163838 39.17000452591084 34.430244399185334 14.556687033265444 6 27.553512580795726 39.019390973508905 18.107916212890544 15.319180232804833 7 26.537961077166777 32.95027155465037 22.970807875084862 17.540959493097986 8 24.668533604887983 32.971373613939804 20.468375198008598 21.89171758316361 9 23.688956777551482 19.864505544240778 23.573546051142795 32.87299162706495 10-11 24.51808868197898 26.284356671797926 28.370720249016035 20.826834397207055 12-13 27.12550916496945 24.147516406426796 27.97960511427925 20.74736931432451 14-15 22.178825865636618 26.71239832367547 28.627074366746204 22.48170144394171 16-17 20.125990042996154 29.54217583163612 28.879582484725052 21.45225164064268 18-19 19.725305498981673 30.35211586331749 29.989335822584295 19.933242815116543 20-21 21.677202521815513 27.533478275014815 29.07098228873005 21.718336914439615 22-23 20.46797918081014 28.013464584747684 29.553830052047974 21.964726182394205 24-25 22.25231952930527 27.816050011314776 29.602964471599908 20.328665987780038 26-27 21.62362709251257 26.484663748170046 31.817365290635585 20.074343868681797 28-29 20.3020132329527 29.61501205721832 30.199484442171183 19.8834902676578 30-31 21.36643038845586 27.73997288684899 29.484335252527995 21.409261472167152 32-33 21.309478042456238 28.00436551144122 30.183500279067978 20.502656167034562 34-35 22.474675465754167 26.720438868068 30.441549888653523 20.363335777524306 36-37 22.14310248338778 26.833710290099212 31.209077745140256 19.814109481372753 38-39 21.165464104910725 28.448645827292236 30.785062301704613 19.600827766092426 40-41 20.71894932975128 28.715649434825096 29.867613783285396 20.69778745213823 42-43 20.35726408689749 28.989958135324734 28.618776872595607 22.034000905182168 44-45 21.524637927132837 27.471232179226067 28.782812853586787 22.221317040054313 46-47 22.52940925215766 28.7599531228387 28.636367365100163 20.07427025990348 48-49 22.182365919891378 28.256732292373844 29.126159764652638 20.434742023082144 50-51 20.14406539941163 28.707541298936412 30.595864448970357 20.5525288526816 52-53 21.246181262729124 29.781313645621182 28.083757637474545 20.888747454175153 54-55 20.47731387191672 28.789545145960627 28.59897601267255 22.1341649694501 56-57 22.03231037786526 27.626445165394724 28.686881373316936 21.654363083423075 58-59 23.537159074483345 27.24541488965383 28.356754496854908 20.860671539007914 60-61 21.55284280756686 28.385555979589416 30.125072386729446 19.936528826114277 62-63 20.637185296716297 28.45393829468865 29.67245028417329 21.236426124421758 64-65 20.265836297320977 30.443228410812083 28.520758856304525 20.77017643556242 66-67 21.189321391001037 28.0838905836996 27.838468411727526 22.888319613571838 68-69 20.743298977237625 29.095151185302274 27.991656322875635 22.169893514584462 70-71 22.344802498536854 28.58223421718047 27.644802017657334 21.42816126662535 72-73 21.564522516406427 27.298455532926003 29.95830504639059 21.178716904276985 74-75 20.367701968771215 30.03241683638832 28.514398053858336 21.085483140982124 76-77 21.31828467979181 28.13294863091197 27.80244399185336 22.74632269744286 78-79 21.57368748585653 28.77899411631591 28.200865580448063 21.446452817379498 80-81 22.30802282983221 27.96914685707933 27.93489135972261 21.787938953365853 82-83 21.23093460058837 27.52848495134646 29.51205023761032 21.728530210454856 84-85 20.764641970447475 28.511027815423017 28.957198141998873 21.767132072130636 86-87 21.105058268899697 28.840375632007813 27.663682739291723 22.390883359800767 88-89 20.555187825299843 28.94110658520027 27.125141434713733 23.37856415478615 90-91 21.037536773025572 26.629327902240323 28.296503733876442 24.03663159085766 92-93 21.40091083955646 28.02616542204119 28.447301425661912 22.12562231274044 94-95 20.8673979419517 28.02049901646133 28.694408284241263 22.417694757345707 96-97 19.501717168088422 28.513900702679724 29.81544650754799 22.168935621683868 98-99 20.22715719994467 29.38421966902327 27.669565251827844 22.719057879204215 100-101 18.971525880163163 27.9525563344026 28.458052580661587 24.617865204772656 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 365.0 1 288.5 2 616.0 3 915.5 4 652.0 5 475.0 6 577.0 7 913.0 8 1308.0 9 1525.0 10 1515.5 11 1509.0 12 1656.0 13 1848.0 14 1994.5 15 2168.0 16 2403.5 17 2627.5 18 2803.0 19 3132.0 20 3502.0 21 3847.0 22 4386.0 23 5181.0 24 6288.0 25 7652.5 26 9337.0 27 11606.5 28 14982.5 29 19102.0 30 23122.5 31 27159.5 32 31421.5 33 35170.0 34 38873.0 35 43070.0 36 46651.5 37 50883.0 38 55370.5 39 57871.0 40 59715.5 41 61503.5 42 63549.5 43 66445.0 44 67253.0 45 67976.0 46 71899.5 47 91576.0 48 135436.5 49 132385.0 50 88242.5 51 65484.0 52 51038.5 53 41626.5 54 35438.0 55 30375.5 56 25589.0 57 20751.5 58 16810.0 59 13757.0 60 11065.0 61 8312.0 62 5988.5 63 4173.5 64 2653.5 65 1665.5 66 998.5 67 532.5 68 314.0 69 188.0 70 99.0 71 59.0 72 37.0 73 24.0 74 13.5 75 11.5 76 11.5 77 7.5 78 3.0 79 1.0 80 1.5 81 1.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04899298483819869 2 0.004525910839556461 3 0.03841366825073546 4 0.0 5 0.0 6 0.001414347137361394 7 0.0 8 0.0 9 0.0 10-11 1.6972165648336727E-4 12-13 0.0 14-15 0.00240439013351437 16-17 0.0 18-19 0.0 20-21 0.012587689522516408 22-23 0.0 24-25 0.0 26-27 0.007778909255487667 28-29 0.010975333785924416 30-31 0.010862186014935505 32-33 0.0061948404616429045 34-35 0.008146639511201629 36-37 0.02132835483140982 38-39 0.010890472957682733 40-41 0.015331522968997511 42-43 0.0 44-45 0.0 46-47 8.486082824168364E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.001442634080108622 58-59 1.9800859923059516E-4 60-61 0.010381307988232632 62-63 0.01586897488119484 64-65 0.04729576827336501 66-67 0.02494908350305499 68-69 0.001923512106811496 70-71 3.111563702195067E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.8286942747227882E-5 82-83 0.0 84-85 8.486082824168364E-5 86-87 0.0010749038243946594 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.6972165648336727E-4 96-97 5.374519121973297E-4 98-99 7.637474541751527E-4 100-101 0.0016972165648336728 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1767600.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.76991167219527 #Duplication Level Percentage of deduplicated Percentage of total 1 80.8945063802723 45.92373981973895 2 12.22608536566437 13.881475726109707 3 2.9640256949545014 5.048024306900527 4 1.169796688602544 2.656370187455717 5 0.6764107821482127 1.9199890178337276 6 0.40667428947254514 1.3852118095625496 7 0.2772060292365753 1.1015873256332251 8 0.20820581446687136 0.9455860557537409 9 0.1536787935375262 0.7851898381513406 >10 0.9183536006595183 9.644476491279114 >50 0.06591301721704855 2.578275380505196 >100 0.0336797276177215 3.484514888174601 >500 0.0015894737891757368 0.6402686599751618 >1k 0.0026822370192340554 3.235575932361049 >5k 9.934211182348353E-4 3.94842679663358 >10k+ 1.986842236469671E-4 2.8212877639317755 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35406 2.0030549898167007 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 14620 0.8271102059289431 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9724 0.5501244625480878 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9480 0.5363204344874406 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7486 0.42351210681149576 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7152 0.4046164290563476 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6736 0.381081692690654 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6435 0.36405295315682284 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6118 0.34611903145508033 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6113 0.34583616202760803 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5744 0.3249603982801539 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5024 0.28422720072414576 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4652 0.2631817153202082 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4464 0.2525458248472505 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3553 0.2010070151618013 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2925 0.16547861507128309 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2919 0.16513917175831636 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2903 0.16423398959040506 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2877 0.1627630685675492 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2728 0.1543335596288753 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2677 0.15144829146865807 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2456 0.13894546277438335 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2372 0.13419325639284907 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2239 0.12666892962208645 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2043 0.11558044806517312 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1811 0.10245530663045939 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.657388549445576E-5 0.0 10-11 0.0 0.0 0.0 1.1314777098891151E-4 0.0 12-13 0.0 0.0 0.0 1.1314777098891151E-4 0.0 14-15 0.0 0.0 0.0 1.6972165648336727E-4 0.0 16-17 0.0 2.828694274722788E-5 0.0 2.2629554197782303E-4 0.0 18-19 0.0 5.657388549445576E-5 0.0 2.5458248472505095E-4 0.0 20-21 0.0 5.657388549445576E-5 0.0 6.788866259334691E-4 0.0 22-23 0.0 5.657388549445576E-5 0.0 0.0018103643358225842 0.0 24-25 0.0 1.1314777098891151E-4 0.0 0.0038187372708757637 0.0 26-27 0.0 1.1314777098891151E-4 0.0 0.00613826657614845 0.0 28-29 0.0 1.6972165648336727E-4 0.0 0.012191672324055217 0.0 30-31 0.0 1.6972165648336727E-4 0.0 0.027466621407558272 0.0 32-33 0.0 1.6972165648336727E-4 0.0 0.051963113826657616 0.0 34-35 0.0 1.6972165648336727E-4 0.0 0.0714245304367504 0.0 36-37 0.0 1.6972165648336727E-4 0.0 0.09459153654673003 0.0 38-39 0.0 1.6972165648336727E-4 0.0 0.13314663951120162 0.0 40-41 0.0 1.6972165648336727E-4 0.0 0.23910952704231725 0.0 42-43 0.0 1.6972165648336727E-4 0.0 0.31206155238741795 0.0 44-45 0.0 1.6972165648336727E-4 0.0 0.38475899524779367 0.0 46-47 0.0 1.6972165648336727E-4 0.0 0.5695575922154333 0.0 48-49 0.0 1.6972165648336727E-4 0.0 0.6574451233310704 0.0 50-51 0.0 1.6972165648336727E-4 0.0 0.7609753337859244 0.0 52-53 0.0 1.6972165648336727E-4 0.0 0.8679565512559403 0.0 54-55 0.0 1.6972165648336727E-4 0.0 0.9936071509391265 0.0 56-57 0.0 1.6972165648336727E-4 0.0 1.4527890925548768 0.0 58-59 0.0 1.6972165648336727E-4 0.0 1.7782869427472279 0.0 60-61 0.0 1.6972165648336727E-4 0.0 1.9515161801312515 2.828694274722788E-5 62-63 0.0 1.6972165648336727E-4 0.0 2.164233989590405 5.657388549445576E-5 64-65 0.0 1.6972165648336727E-4 0.0 2.377602398732745 5.657388549445576E-5 66-67 0.0 1.6972165648336727E-4 0.0 2.8329656030776196 5.657388549445576E-5 68-69 0.0 1.6972165648336727E-4 0.0 3.30040733197556 5.657388549445576E-5 70-71 0.0 1.6972165648336727E-4 0.0 3.4467073998642226 5.657388549445576E-5 72-73 0.0 2.828694274722788E-4 0.0 3.5758938673908123 5.657388549445576E-5 74-75 0.0 2.828694274722788E-4 0.0 3.9546843177189412 5.657388549445576E-5 76-77 0.0 2.828694274722788E-4 0.0 4.3284114052953155 5.657388549445576E-5 78-79 0.0 2.828694274722788E-4 0.0 4.620672097759674 5.657388549445576E-5 80-81 0.0 2.828694274722788E-4 0.0 4.861846571622539 5.657388549445576E-5 82-83 0.0 2.828694274722788E-4 0.0 5.33500226295542 5.657388549445576E-5 84-85 0.0 2.828694274722788E-4 0.0 6.154616429056348 5.657388549445576E-5 86-87 0.0 2.828694274722788E-4 0.0 7.156511654220412 5.657388549445576E-5 88-89 0.0 2.828694274722788E-4 0.0 8.23642226748133 5.657388549445576E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 310 0.0 58.233105 2 TAACGCA 390 0.0 51.14579 4 TACCTGG 2125 0.0 50.300426 2 ATAACGC 415 0.0 46.932266 3 ACCTGGG 2440 0.0 42.05326 3 GTACCTG 2720 0.0 40.18981 1 GTATAAC 595 0.0 34.348557 1 GTACACG 200 0.0 33.270172 1 TACGCAG 470 0.0 31.324852 4 CCTGGGG 1780 0.0 29.61612 4 AGTACGC 540 0.0 27.271938 2 TACACGG 250 0.0 26.603334 2 GAGTACG 600 0.0 26.140852 1 GTACGCA 560 0.0 25.4489 3 GCTCTAG 530 0.0 23.316025 1 TATCACG 300 7.2759576E-12 22.169449 2 CTGGGGG 1115 0.0 22.148977 5 TCACGCA 305 9.094947E-12 21.799845 4 TACCGAC 110 0.0028806038 21.58751 7 GTACCGG 140 4.8333476E-4 20.369493 1 >>END_MODULE