##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003863_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 832940 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.659787019473193 31.0 31.0 34.0 27.0 34.0 2 30.513774101375848 31.0 31.0 34.0 26.0 34.0 3 30.620816625447212 31.0 31.0 34.0 27.0 34.0 4 34.02090306624727 37.0 35.0 37.0 30.0 37.0 5 34.04959180733306 37.0 35.0 37.0 30.0 37.0 6 34.191665666194446 37.0 35.0 37.0 30.0 37.0 7 34.21351117727568 37.0 35.0 37.0 30.0 37.0 8 34.20603644920402 37.0 35.0 37.0 30.0 37.0 9 35.62192114678128 39.0 35.0 39.0 30.0 39.0 10-11 35.42597305928398 38.5 35.0 39.0 29.5 39.0 12-13 35.318354263212235 38.0 35.0 39.0 29.0 39.0 14-15 36.14751962926501 39.0 36.0 41.0 27.5 41.0 16-17 36.29132950752755 39.0 36.0 41.0 29.0 41.0 18-19 36.30036437198358 39.0 36.0 41.0 29.0 41.0 20-21 36.156797608471194 39.0 36.0 41.0 27.5 41.0 22-23 36.04576320023051 39.0 35.5 40.5 27.0 41.0 24-25 35.74736295531491 39.0 35.0 40.5 27.0 41.0 26-27 35.51273381035848 38.5 34.5 40.0 26.5 41.0 28-29 35.38865164357577 38.0 34.5 40.0 27.0 41.0 30-31 35.13816961605878 38.0 34.0 40.0 25.5 41.0 32-33 34.74915720219944 38.0 34.0 40.0 25.0 41.0 34-35 34.57032439311355 38.0 34.0 40.0 24.0 41.0 36-37 34.33891696880927 38.0 33.5 40.0 24.0 41.0 38-39 34.30318690421879 38.0 33.0 40.0 24.0 41.0 40-41 34.56772396571182 38.0 34.0 40.0 24.0 41.0 42-43 34.37531935073355 38.0 33.5 40.0 23.5 41.0 44-45 34.096144980430765 38.0 33.0 40.0 21.0 41.0 46-47 33.799673445866446 38.0 33.0 40.0 18.5 41.0 48-49 33.44500444209667 38.0 33.0 40.0 15.0 41.0 50-51 32.503018824885345 37.0 31.0 39.5 13.0 40.5 52-53 32.70441388335294 37.5 32.0 39.5 13.0 40.5 54-55 33.01878526664586 38.0 32.0 40.0 11.0 41.0 56-57 32.59348512497899 37.5 31.5 40.0 8.0 41.0 58-59 32.18072550243715 37.0 31.0 40.0 7.0 41.0 60-61 31.659246164189497 36.0 30.0 39.5 4.0 41.0 62-63 31.52832677023555 36.0 30.0 39.0 2.0 41.0 64-65 31.25089562273393 36.0 30.0 39.0 2.0 40.5 66-67 30.674689053233124 35.0 29.0 39.0 2.0 40.0 68-69 30.20186508031791 35.0 29.0 38.0 2.0 40.0 70-71 29.596513554397674 34.5 28.0 37.0 2.0 39.0 72-73 29.077470165918314 34.0 27.0 37.0 2.0 39.0 74-75 28.73669171849113 34.0 26.5 36.0 2.0 39.0 76-77 28.304200182486134 34.0 26.0 36.0 2.0 37.5 78-79 27.60087461281725 34.0 26.0 35.0 2.0 37.0 80-81 26.906732777871156 33.0 25.0 35.0 2.0 37.0 82-83 26.370140106130094 32.5 23.0 35.0 2.0 36.0 84-85 26.060525367973682 32.0 23.0 35.0 2.0 36.0 86-87 26.047201479098135 32.0 23.0 35.0 2.0 36.0 88-89 25.639696736859797 32.0 20.0 35.0 2.0 35.0 90-91 25.15208658486806 32.0 19.0 35.0 2.0 35.0 92-93 24.84168907724446 32.0 18.0 35.0 2.0 35.0 94-95 24.542522870795015 32.0 15.0 35.0 2.0 35.0 96-97 24.104482315652987 31.0 8.5 34.0 2.0 35.0 98-99 23.909260570989506 31.0 7.0 34.0 2.0 35.0 100-101 23.53888155209259 31.0 2.0 34.0 2.0 35.0 102-103 22.89118123754412 31.0 2.0 34.0 2.0 35.0 104-105 22.318694023579127 30.0 2.0 34.0 2.0 35.0 106-107 21.737364636108243 29.0 2.0 34.0 2.0 35.0 108-109 21.495016447763344 29.0 2.0 34.0 2.0 35.0 110-111 21.194320719379547 29.0 2.0 34.0 2.0 35.0 112-113 20.5043454510529 29.0 2.0 34.0 2.0 35.0 114-115 19.95327334501885 28.0 2.0 34.0 2.0 35.0 116-117 19.290083919610055 26.0 2.0 34.0 2.0 35.0 118-119 18.493514538862343 25.0 2.0 34.0 2.0 35.0 120-121 16.994682089946455 21.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.27361533874979926 1101 2 0.16540023673756465 1101 3 0.42518073504649934 1101 4 0.44808725151442275 1101 5 0.590027816367531 1101 6 0.665804164420905 1101 7 0.4762346203182801 1101 8 0.5821140413143482 1101 9 0.5842915701894142 1101 10-11 0.5831134700349097 1101 12-13 0.7211629234362036 1101 14-15 0.9099983741596986 1101 16-17 0.7656281946089294 1101 18-19 0.89109953123954 1101 20-21 1.0600691198789534 1101 22-23 0.9530609953707767 1101 24-25 0.9833598263540537 1101 26-27 0.8500912267928769 1101 28-29 1.0054777120055078 1101 30-31 0.9810503606289274 1101 32-33 0.821372397760733 1101 34-35 0.6858048379962938 1101 36-37 0.672204474635798 1101 38-39 0.8840080133804804 1101 40-41 0.8143586840153816 1101 42-43 0.9362389566464913 1101 44-45 1.0466607089575604 1101 46-47 0.57400749732777 1101 48-49 0.7045924360105502 1101 50-51 0.5522725877956205 1101 52-53 0.5869981615745559 1101 54-55 0.5636951836804371 1101 56-57 0.5072896738626156 1101 58-59 0.20641850725809974 1101 60-61 0.18193108863687968 1101 62-63 0.533277869473352 1101 64-65 0.6909824989152433 1101 66-67 0.27981732021021344 1101 68-69 0.1650882967210805 1101 70-71 0.04088217442020081 1101 72-73 -0.03018833314969882 1101 74-75 0.11306549491610696 1101 76-77 0.1902979964941096 1101 78-79 0.12060983200574427 1101 80-81 0.342561982792045 1101 82-83 0.40648059430656147 1101 84-85 0.45673777194034315 1101 86-87 0.4774338727266816 1101 88-89 0.4654148287911113 1101 90-91 0.42927876766631456 1101 92-93 0.49769226059401106 1101 94-95 0.19628550831641078 1101 96-97 0.3735328438161041 1101 98-99 0.5536228741750797 1101 100-101 0.7366637616436265 1101 102-103 0.32840750989676337 1101 104-105 0.5328915643869792 1101 106-107 0.5050810952018843 1101 108-109 0.458821331443513 1101 110-111 0.5055774747825623 1101 112-113 0.5765474349490027 1101 114-115 0.12020313849364328 1101 116-117 0.19013666087050396 1101 118-119 0.3910077463658794 1101 120-121 0.29683715302349967 1102 1 0.5703524711509864 1102 2 0.6924182837620769 1102 3 0.874276704148933 1102 4 1.098802634264402 1102 5 1.1162963098173364 1102 6 0.8430588877107112 1102 7 0.7132069277780175 1102 8 0.86981768840689 1102 9 0.9791813784653698 1102 10-11 0.8459259546160496 1102 12-13 1.0404669723902416 1102 14-15 1.0055811520640319 1102 16-17 0.9393325114706315 1102 18-19 1.0906886415068513 1102 20-21 1.0530050498450336 1102 22-23 1.12401327484298 1102 24-25 1.07964439216412 1102 26-27 1.1495006121694402 1102 28-29 1.0326374813445653 1102 30-31 1.1577523668920051 1102 32-33 0.7716861289733359 1102 34-35 1.0111892991418117 1102 36-37 1.0702074497546121 1102 38-39 0.8756768408750233 1102 40-41 0.7655878364004636 1102 42-43 0.6845007587034644 1102 44-45 0.682340928826612 1102 46-47 0.9810786009876722 1102 48-49 0.9786629213412397 1102 50-51 0.7109832086758274 1102 52-53 0.7468105422259939 1102 54-55 0.6933448566168252 1102 56-57 0.7336391065009451 1102 58-59 0.6555858624152648 1102 60-61 0.8063459591919191 1102 62-63 0.6205851140031342 1102 64-65 0.6695698318797447 1102 66-67 0.33678759106779665 1102 68-69 0.3670158251783988 1102 70-71 0.42336078222416873 1102 72-73 0.22906167236172692 1102 74-75 0.3806744924984322 1102 76-77 0.23588691569874243 1102 78-79 0.20906660197573146 1102 80-81 0.1578988567337234 1102 82-83 0.07918078232482983 1102 84-85 0.09146635271209291 1102 86-87 0.2906060695380326 1102 88-89 0.08112637454064853 1102 90-91 0.1839857856503535 1102 92-93 0.2841917929103701 1102 94-95 0.047625099127348136 1102 96-97 0.4354397752970378 1102 98-99 0.36419787834801554 1102 100-101 0.2685150644916128 1102 102-103 0.12462394401635635 1102 104-105 -0.08134291990651832 1102 106-107 -0.023398791252297713 1102 108-109 0.023380044898839714 1102 110-111 -0.13962071075189542 1102 112-113 -0.28750097319446866 1102 114-115 -0.2619222133188792 1102 116-117 -0.33936940012047856 1102 118-119 -0.8560620135565173 1102 120-121 -0.5570266696787378 1103 1 0.5338985008825361 1103 2 0.5125425092640192 1103 3 0.4586335089737865 1103 4 0.7406041770896508 1103 5 0.8366185490655624 1103 6 0.800734405107633 1103 7 0.6954719734915642 1103 8 0.5529880490365571 1103 9 0.5801569210918416 1103 10-11 0.8235011435919333 1103 12-13 0.829549020855012 1103 14-15 1.1510677383529213 1103 16-17 0.8473751679784911 1103 18-19 0.8442712923134863 1103 20-21 1.0403760760542795 1103 22-23 0.9544406590628256 1103 24-25 0.7972148628863707 1103 26-27 0.8977998018749105 1103 28-29 0.9422834703374932 1103 30-31 0.9242632510949207 1103 32-33 0.9540149584860131 1103 34-35 1.0235244742687541 1103 36-37 0.9169373832512591 1103 38-39 0.9227900232606459 1103 40-41 0.791820015982438 1103 42-43 0.8944805204534916 1103 44-45 0.9824808381531369 1103 46-47 0.9659747284154747 1103 48-49 0.8623115512455755 1103 50-51 0.7535064695570242 1103 52-53 0.7260174605838259 1103 54-55 0.8248831055986372 1103 56-57 0.8195539179946962 1103 58-59 0.989895976188528 1103 60-61 0.9276121768235477 1103 62-63 1.1161857793642724 1103 64-65 1.0140940910210894 1103 66-67 0.8730547971298819 1103 68-69 0.5400561389422656 1103 70-71 0.6593313499327458 1103 72-73 0.5600542640482082 1103 74-75 0.48050706002570465 1103 76-77 0.6068060904849197 1103 78-79 0.6722571244021793 1103 80-81 0.4469306645347828 1103 82-83 0.5351410179317 1103 84-85 0.5655678366683894 1103 86-87 0.6200532147215405 1103 88-89 0.7072353552777209 1103 90-91 0.8245209360938368 1103 92-93 0.533298655894253 1103 94-95 0.6494257900456937 1103 96-97 0.4061128800499141 1103 98-99 0.5465736719593686 1103 100-101 0.458001004205002 1103 102-103 0.6478029110901566 1103 104-105 0.659936229235079 1103 106-107 0.3451421434206452 1103 108-109 0.44394245636332386 1103 110-111 0.7886880465234469 1103 112-113 0.7259760848433281 1103 114-115 0.6771101951857723 1103 116-117 0.7268073836688238 1103 118-119 0.7115608275966991 1103 120-121 0.8526711982603992 1104 1 0.49146496539204776 1104 2 0.539002346561098 1104 3 0.6528188950267726 1104 4 0.7646191190076408 1104 5 0.9063032205449133 1104 6 0.8753787214988549 1104 7 0.7573547376089707 1104 8 0.9290461676924266 1104 9 0.8983391556918647 1104 10-11 0.9999676227593639 1104 12-13 1.0865229847232314 1104 14-15 1.366913637799584 1104 16-17 1.1041212469846897 1104 18-19 0.953278126569046 1104 20-21 0.7675870172760213 1104 22-23 0.8314131800419773 1104 24-25 1.0696437719706253 1104 26-27 1.16530170282401 1104 28-29 1.1928428761420307 1104 30-31 1.2678649541620928 1104 32-33 1.2840013999093003 1104 34-35 1.1613890900480044 1104 36-37 0.9910984463169683 1104 38-39 0.8781858558305515 1104 40-41 1.0976460814300566 1104 42-43 1.2824127000863115 1104 44-45 1.213571547779722 1104 46-47 1.2899897848484585 1104 48-49 1.3122971223548134 1104 50-51 0.9960939132156454 1104 52-53 1.2569639958177987 1104 54-55 1.2520223604265865 1104 56-57 1.4480471982541445 1104 58-59 1.2698221827186273 1104 60-61 1.2597577986858433 1104 62-63 1.3798021861582157 1104 64-65 1.3999335089184122 1104 66-67 1.5100679938391792 1104 68-69 1.4261784089045193 1104 70-71 1.3162478272679365 1104 72-73 1.1854659454949434 1104 74-75 1.3819342605460605 1104 76-77 1.4460018286944525 1104 78-79 1.4859996122177819 1104 80-81 1.6984279482388445 1104 82-83 1.6856475178034422 1104 84-85 1.8325704521911312 1104 86-87 1.854417335795965 1104 88-89 1.775212608016922 1104 90-91 1.8353179834389195 1104 92-93 1.5639577584630509 1104 94-95 1.572367497327015 1104 96-97 1.2100105127957086 1104 98-99 1.4290114216020804 1104 100-101 1.6215519084154906 1104 102-103 1.5917243400542063 1104 104-105 1.8855033075450187 1104 106-107 1.6629012450733214 1104 108-109 1.3464105982886387 1104 110-111 1.5693042059649756 1104 112-113 1.5069220475022753 1104 114-115 1.6801517137463655 1104 116-117 1.4430678274373783 1104 118-119 1.3353607103333331 1104 120-121 1.270295401235959 1105 1 0.7164917809013431 1105 2 0.8262163144619414 1105 3 0.7477060712543278 1105 4 1.1545414533473846 1105 5 1.095898802938045 1105 6 0.9609350936583922 1105 7 1.0449212797987073 1105 8 1.0421480269010743 1105 9 1.1288929456171815 1105 10-11 1.3020145399689653 1105 12-13 1.3731013257370535 1105 14-15 1.533889841517997 1105 16-17 1.423767083628583 1105 18-19 1.2876476641504837 1105 20-21 1.3375382527757793 1105 22-23 1.1177804730659773 1105 24-25 1.1537749494694296 1105 26-27 1.2599629335565723 1105 28-29 1.4641636889499097 1105 30-31 1.4090163326779006 1105 32-33 1.4220317510931508 1105 34-35 1.2639534472703104 1105 36-37 1.3887972783525626 1105 38-39 1.1559374250344234 1105 40-41 1.401743888517295 1105 42-43 1.283976938128582 1105 44-45 1.1774922731889035 1105 46-47 1.408377775472971 1105 48-49 1.5008548452214967 1105 50-51 1.4291395591714675 1105 52-53 1.3746741499573218 1105 54-55 1.4718276003757396 1105 56-57 1.4265327178791267 1105 58-59 1.4877975124500864 1105 60-61 1.3423917769156155 1105 62-63 1.388201896153447 1105 64-65 1.6276890507903268 1105 66-67 1.4733580581963999 1105 68-69 1.632883775979046 1105 70-71 1.588181920705086 1105 72-73 1.2049841601779256 1105 74-75 1.1779799660919075 1105 76-77 1.4453364067971073 1105 78-79 1.365732053025031 1105 80-81 1.5971373277080971 1105 82-83 1.438093191567944 1105 84-85 1.6119011569424408 1105 86-87 1.4883856339049721 1105 88-89 1.3264351966074202 1105 90-91 1.2796286262166099 1105 92-93 1.3400134949957057 1105 94-95 1.5373434626853495 1105 96-97 1.2549910467222958 1105 98-99 1.3805299739618455 1105 100-101 1.523057053020608 1105 102-103 1.844587143963313 1105 104-105 1.7439770084825668 1105 106-107 1.540044002899478 1105 108-109 1.4332903654902935 1105 110-111 1.7894024301823634 1105 112-113 1.921244012383891 1105 114-115 2.0494112084229883 1105 116-117 1.9806045746167804 1105 118-119 1.3678174082516534 1105 120-121 1.2107475901760516 1106 1 0.32688420924545625 1106 2 0.5649900533803347 1106 3 0.5564256678735333 1106 4 0.8355513518822306 1106 5 0.3555648565430545 1106 6 0.7367663432709293 1106 7 0.47771870080674717 1106 8 0.537906535714697 1106 9 0.5896759424554361 1106 10-11 0.8781134013274823 1106 12-13 0.8922965273241275 1106 14-15 0.7441123002396708 1106 16-17 0.6929906995902186 1106 18-19 0.564757560618844 1106 20-21 0.43320428571578873 1106 22-23 0.35006798520665683 1106 24-25 0.6747060110304588 1106 26-27 0.6706823855207773 1106 28-29 0.7122418439787452 1106 30-31 0.602407149152036 1106 32-33 0.8664971475511791 1106 34-35 0.566339221484526 1106 36-37 0.8470643114785545 1106 38-39 0.7769000007199907 1106 40-41 0.7938054288863654 1106 42-43 0.8567564839118873 1106 44-45 0.7080893883549493 1106 46-47 0.9657257327377522 1106 48-49 1.1075895686204689 1106 50-51 0.6945582320651909 1106 52-53 0.759882659804191 1106 54-55 0.9601777068004864 1106 56-57 1.0475934523132082 1106 58-59 1.187447240705339 1106 60-61 1.1023988030155074 1106 62-63 1.1944597746845815 1106 64-65 1.1903091661010343 1106 66-67 1.0699126861230823 1106 68-69 0.7975373272592456 1106 70-71 0.7297268360819125 1106 72-73 0.6801577461753219 1106 74-75 0.7922264168782078 1106 76-77 0.9239765464925078 1106 78-79 1.0096146964917914 1106 80-81 0.7783022137406235 1106 82-83 0.6310216286740165 1106 84-85 0.48568192196072957 1106 86-87 0.9764696784587166 1106 88-89 1.1554555722971145 1106 90-91 0.745180071733337 1106 92-93 0.5745741571022975 1106 94-95 0.8062906842761386 1106 96-97 0.7610484747564321 1106 98-99 0.9914002804237931 1106 100-101 1.2211078269248432 1106 102-103 0.9345393044890287 1106 104-105 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1.5080070988115999 2209 100-101 1.5808281728939342 2209 102-103 1.512059190374476 2209 104-105 1.5790913066157444 2209 106-107 1.5971015910625042 2209 108-109 1.6468326096722663 2209 110-111 1.822495997641326 2209 112-113 1.6455474457632029 2209 114-115 1.6520411299622175 2209 116-117 1.6575033340584646 2209 118-119 1.8192933710482109 2209 120-121 1.8041470614862014 2210 1 0.4518973468575638 2210 2 0.543413715095582 2210 3 0.6827219122681534 2210 4 0.7668416405593632 2210 5 0.9294376494242229 2210 6 0.9685225792574741 2210 7 0.692733239764145 2210 8 0.7197923961407042 2210 9 1.1055724531056583 2210 10-11 0.9247891475438479 2210 12-13 1.1485347034732314 2210 14-15 1.4676005990064809 2210 16-17 1.1654257997864121 2210 18-19 0.9472503787242204 2210 20-21 1.0562117478794661 2210 22-23 1.0314064451281837 2210 24-25 1.0747791437378638 2210 26-27 1.348929127392978 2210 28-29 1.3038679300213403 2210 30-31 1.2650026158000216 2210 32-33 1.225996954866197 2210 34-35 1.1372275868152428 2210 36-37 1.2350201866186907 2210 38-39 1.16457122759779 2210 40-41 1.1455649930679854 2210 42-43 1.1674982334914858 2210 44-45 1.3392618764435156 2210 46-47 1.2582346663139816 2210 48-49 0.9894758669668846 2210 50-51 0.7515491933880583 2210 52-53 1.1077688180160763 2210 54-55 1.2388724412455545 2210 56-57 1.2247848060127637 2210 58-59 0.9907947042703569 2210 60-61 1.079716715711701 2210 62-63 0.8046405482271837 2210 64-65 0.8406494302546186 2210 66-67 1.0759691253046952 2210 68-69 1.1213225360596937 2210 70-71 1.1982184630437978 2210 72-73 1.0612404605811498 2210 74-75 0.9591332098995089 2210 76-77 0.8534472758927301 2210 78-79 0.6987218643729562 2210 80-81 0.7010377273336701 2210 82-83 0.808307135260339 2210 84-85 1.1543141213721633 2210 86-87 0.9402033002356198 2210 88-89 1.0651169722410607 2210 90-91 1.2774482366716775 2210 92-93 1.321197790790638 2210 94-95 1.303560250559773 2210 96-97 1.4033025386577798 2210 98-99 1.2014050099641516 2210 100-101 1.3439050872948002 2210 102-103 1.3009275997524838 2210 104-105 1.5217842881484671 2210 106-107 1.620639661021599 2210 108-109 1.8256502265214003 2210 110-111 1.82841317687015 2210 112-113 1.838161945131585 2210 114-115 1.6290505553411911 2210 116-117 1.651142989075307 2210 118-119 1.7335288137816107 2210 120-121 1.827912588735959 2211 1 0.23954238089221747 2211 2 0.6026204756153852 2211 3 0.47302203603052817 2211 4 0.4847987878365885 2211 5 0.6260283827162993 2211 6 0.5233067686139066 2211 7 0.4402947341948362 2211 8 0.37105709292288225 2211 9 0.6476477934521938 2211 10-11 0.39669218745721935 2211 12-13 0.5705576381885749 2211 14-15 0.7385357784619302 2211 16-17 0.7802415098730435 2211 18-19 0.4080621825608546 2211 20-21 0.48483373117154116 2211 22-23 0.49779142346976357 2211 24-25 0.6414899652106385 2211 26-27 0.5069253371702018 2211 28-29 0.7122895729668812 2211 30-31 0.6707595000822053 2211 32-33 0.8095220554231233 2211 34-35 0.7141758773474223 2211 36-37 1.0000157389082958 2211 38-39 1.1114491282784797 2211 40-41 1.0071928550729368 2211 42-43 0.9003774491474275 2211 44-45 1.1280101360351011 2211 46-47 1.2697194280714115 2211 48-49 1.2387033190089625 2211 50-51 0.976746439675189 2211 52-53 1.045509574513595 2211 54-55 1.1097969848717923 2211 56-57 0.6870952948741476 2211 58-59 0.8468087822406574 2211 60-61 0.6985605043874408 2211 62-63 1.204160969019263 2211 64-65 1.3223142275937256 2211 66-67 1.1736466469631104 2211 68-69 1.1132335045002861 2211 70-71 1.1631260280190467 2211 72-73 1.0099506245662973 2211 74-75 0.9943542417683204 2211 76-77 0.9131365163011473 2211 78-79 0.7570584206848352 2211 80-81 0.7826599813559483 2211 82-83 0.9766694109410317 2211 84-85 0.8229393921023593 2211 86-87 0.8830917980697777 2211 88-89 0.9716512389489829 2211 90-91 0.8432675822781128 2211 92-93 1.1320676082411332 2211 94-95 1.1768208090374976 2211 96-97 1.1192756203409466 2211 98-99 0.881779004394847 2211 100-101 0.8046124666264838 2211 102-103 1.0210186811262467 2211 104-105 1.150491358074209 2211 106-107 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-1.5209266146755347 2316 76-77 -1.5016271242420132 2316 78-79 -1.1312607097356668 2316 80-81 -1.3356125175357327 2316 82-83 -1.3248576074324454 2316 84-85 -1.3021948377385328 2316 86-87 -1.3322892176437335 2316 88-89 -1.053701370938871 2316 90-91 -1.1746313717963979 2316 92-93 -0.9820532068254515 2316 94-95 -0.9720708711753012 2316 96-97 -1.161976534725305 2316 98-99 -1.1375755430001924 2316 100-101 -1.0608112399232752 2316 102-103 -0.6392016692670026 2316 104-105 -0.8583349723200229 2316 106-107 -1.0563643164866932 2316 108-109 -1.346685523115827 2316 110-111 -1.2399907345622694 2316 112-113 -0.7977980660106212 2316 114-115 -0.7799615132222435 2316 116-117 -1.050253997346747 2316 118-119 -0.8862110286727827 2316 120-121 -0.9235530647673578 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13476.0 3 2100.0 4 1768.0 5 2018.0 6 2457.0 7 2695.0 8 3500.0 9 4462.0 10 6312.0 11 8133.0 12 7885.0 13 8292.0 14 9812.0 15 10102.0 16 9067.0 17 9449.0 18 9932.0 19 10393.0 20 11655.0 21 12738.0 22 13782.0 23 15362.0 24 16731.0 25 18442.0 26 20246.0 27 22765.0 28 25697.0 29 29027.0 30 32907.0 31 38449.0 32 44446.0 33 53186.0 34 64295.0 35 78567.0 36 95556.0 37 90477.0 38 26628.0 39 131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.27988492922665 16.36320871353529 11.106862632756572 20.250043724481486 2 13.279925398440149 24.898512813060115 34.93799350869544 26.883568279804294 3 27.614543490868847 24.04841211319891 29.812122158736614 18.524922237195625 4 10.774918201263024 15.177705713383036 44.348170286631486 29.69920579872245 5 9.976840838572292 41.61845372344354 33.45209515957788 14.952610278406292 6 24.302000648963837 35.701970622278836 18.805422238150356 21.190606490606974 7 25.318522804719645 31.862327937158554 25.889533567612784 16.92961569050902 8 21.518107872567057 25.625544688680158 20.572649365740293 32.28369807301249 9 20.186809781793006 22.531554561431538 29.467869298513172 27.81376635826228 10-11 25.682544907932986 21.916475238609326 28.21211842477906 24.188861428678628 12-13 32.40777274817845 20.943816416934958 26.49076517150396 20.157645663382635 14-15 24.53699360992782 24.127893430613646 32.29960899463631 19.035503964822226 16-17 19.12291247102777 27.929986023112406 32.12910552156672 20.817995984293102 18-19 17.683670040295844 34.070970339714265 27.727417220948997 20.51794239904089 20-21 21.030076699337144 27.617331579928845 25.31574600821969 26.036845712514317 22-23 18.08119443676043 27.342183299748978 27.787809031033394 26.788813232457194 24-25 24.580816233395613 26.104603622202895 26.432227594859942 22.88235254954155 26-27 22.675042883826897 21.879044002377526 35.32029406744473 20.12561904635085 28-29 17.881469471968675 32.11704727696293 29.044356471848197 20.957126779220197 30-31 20.301858095937543 25.82565055154371 28.2844232721839 25.588068080334843 32-33 22.451645947898044 25.84361905719058 30.513530416419705 21.19120457849167 34-35 25.835732645480057 23.11592689136944 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20.820454435347123 60-61 23.11756447203133 25.964218613153278 32.61947419362264 18.298742721192763 62-63 19.413996601790657 27.788293738018165 32.04967973754101 20.74802992265017 64-65 18.086718553701868 33.82689588990503 26.813609297284863 21.272776259108234 66-67 20.60231072059559 27.912286586510344 24.807974783332103 26.677427909561967 68-69 18.26312022120673 28.296814763587125 26.972226831368623 26.46783818383752 70-71 24.554651664949535 26.615631270778934 25.334537444026722 23.49517962024481 72-73 22.779992707087814 22.865460307537987 33.362953217543215 20.991593767830988 74-75 17.834135242974682 32.22010950715722 27.601219007651206 22.344536242216893 76-77 20.479944555141643 26.168919013066983 27.21329610353261 26.137840328258765 78-79 22.941599598103718 26.44419356226635 28.865873176028995 21.748333663600935 80-81 25.416148917727927 25.104226649775423 27.271740909338877 22.207883523157772 82-83 23.101216925093333 23.676019067967356 32.072275037311236 21.150488969628075 84-85 21.41135226022784 28.234555876980032 30.172733351746594 20.181358511045534 86-87 21.03696480969497 30.07228229765045 27.660409468466202 21.230343424188376 88-89 19.339488657870753 28.482113927002175 24.98999179357355 27.188405621553528 90-91 21.30437863844206 25.19220786615197 25.296511638471735 28.20690185693423 92-93 24.451340602158115 27.892555601785652 27.0282546644452 20.627849131611033 94-95 22.572555507847678 25.994744596981807 28.74992505691133 22.68277483825919 96-97 16.90970680740525 27.753999003088246 33.0147980224081 22.3214961670984 98-99 19.936814110505637 29.225866837100963 27.93858307308784 22.898735979305563 100-101 18.891237226748274 25.812917635128873 28.52194645910833 26.773898679014525 102-103 23.237112615365234 23.16086360632982 28.56152217005043 25.040501608254516 104-105 27.54123179124769 23.43316165405566 27.55461490248799 21.470991652208667 106-107 23.449982317347892 24.872648570611915 31.531802128700644 20.14556698333955 108-109 19.7642266969937 26.07888662001342 31.494635845487156 22.662250837505724 110-111 19.203207034159515 31.524976231855973 26.440773418434393 22.83104331555012 112-113 21.267207286865837 26.860748914419297 24.837238597617475 27.034805201097388 114-115 18.792758025911606 26.776577807494405 26.853796621994825 27.576867544599164 116-117 24.183161344451275 25.821593968929935 25.28627289441084 24.708971792207947 118-119 22.528326230953052 23.706140151618406 31.03967338136905 22.725860236059496 120-121 19.584425243117106 30.978603514258772 26.150661444476274 23.286309798147848 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1841.0 1 2172.5 2 1823.5 3 1039.5 4 811.0 5 690.5 6 725.5 7 860.5 8 999.5 9 1022.5 10 979.5 11 952.5 12 961.5 13 1028.5 14 1121.0 15 1177.5 16 1219.5 17 1301.5 18 1399.5 19 1517.5 20 1612.0 21 1751.5 22 1984.5 23 2324.0 24 2746.0 25 3210.5 26 3777.5 27 4534.5 28 5610.5 29 6760.5 30 7912.5 31 9169.5 32 10272.5 33 11262.5 34 12362.5 35 13502.0 36 14620.0 37 15787.0 38 16925.5 39 17753.5 40 18559.0 41 19438.0 42 20032.0 43 21114.0 44 22153.5 45 24224.0 46 30098.5 47 53171.5 48 109167.0 49 114504.5 50 63173.5 51 35171.0 52 23031.0 53 17356.5 54 14440.5 55 12354.0 56 10453.0 57 8602.5 58 7087.0 59 5755.5 60 4646.0 61 3649.5 62 2683.0 63 1899.5 64 1234.5 65 764.0 66 470.0 67 315.0 68 212.5 69 147.5 70 103.5 71 68.0 72 47.0 73 41.5 74 32.0 75 28.0 76 25.0 77 18.0 78 11.5 79 9.5 80 10.0 81 7.0 82 5.0 83 4.0 84 4.0 85 2.5 86 1.5 87 1.5 88 1.0 89 1.0 90 0.5 91 0.5 92 1.0 93 1.5 94 1.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4664201503109468 2 0.8668091339112061 3 0.8441184238960789 4 0.8196268638797513 5 0.8304319638869547 6 0.8413571205609047 7 0.8237087905491393 8 0.8139842005426562 9 0.8106226138737485 10-11 0.8036593272024396 12-13 0.8067808005378538 14-15 0.8087617355391745 16-17 0.8324128988882752 18-19 0.8611064422407376 20-21 0.8545633539030423 22-23 0.8296515955531011 24-25 0.8212476288808317 26-27 0.8249093572166062 28-29 0.8465795855643865 30-31 0.8549835522366557 32-33 0.8498211155665474 34-35 0.8623070089082048 36-37 0.871551372247701 38-39 0.8582250822388168 40-41 0.8677095589118065 42-43 0.8615266405743511 44-45 0.8459192738972795 46-47 0.8398564122265708 48-49 0.8445386222296924 50-51 0.8926213172617474 52-53 0.8489807188993205 54-55 0.8628472639085649 56-57 0.9106898456071266 58-59 0.8764136672509424 60-61 0.891660863927774 62-63 0.8657286239104858 64-65 0.8533027589022019 66-67 0.8568444305712295 68-69 0.8748529305832352 70-71 0.8925612889283742 72-73 0.898143923932096 74-75 0.9021658222681106 76-77 0.9142915456095277 78-79 0.9426249189617499 80-81 0.9670564506447044 82-83 0.9348212356232141 84-85 0.8941820539294548 86-87 0.8833169255888779 88-89 0.884517492256345 90-91 0.8964031022642687 92-93 0.8835570389223714 94-95 0.878034432252023 96-97 0.8872787955915192 98-99 0.9121305256080869 100-101 0.9044468989362978 102-103 0.8830768122553845 104-105 0.8731121089154081 106-107 0.8730520805820347 108-109 0.873292193915528 110-111 0.8710111172473407 112-113 0.8818162172545441 114-115 0.8840972939227315 116-117 0.9061276922707518 118-119 0.9019257089346172 120-121 0.8836770955891181 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 832940.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.33099045624171 #Duplication Level Percentage of deduplicated Percentage of total 1 85.9780428615057 44.13338097570289 2 9.04978518617986 9.290688740456721 3 1.986753293516878 3.059460430452649 4 0.9140513096171026 1.876766362018829 5 0.4787681451952004 1.2287821545883688 6 0.32652825364184407 1.0056611202829662 7 0.22010625129661807 0.7908790319266087 8 0.15696806051224838 0.6445860812871195 9 0.12300015722529348 0.5682347906982992 >10 0.6838949098630176 6.356243739405995 >50 0.03951289994328221 1.3963247360771576 >100 0.027682636476975664 2.837946413327528 >500 0.0066249044559641024 2.4219766069367066 >1k 0.005915093264253663 6.941412646317346 >5k 0.0014196223834208792 4.559757784115521 >10k+ 9.464149222805861E-4 12.887898386405356 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 39144 4.699498163132999 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35236 4.230316709486877 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 21214 2.546882128364588 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10522 1.2632362475088244 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8803 1.0568588373712393 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6404 0.7688428938458952 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6143 0.7375081038250053 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5607 0.6731577304487718 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5339 0.640982543760655 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5248 0.6300573870867049 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4540 0.5450572670300382 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4391 0.5271688236847792 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3737 0.44865176363243453 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3540 0.42500060028333375 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3333 0.40014887026676593 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3303 0.3965471702643648 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2525 0.30314308353542874 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2440 0.29293826686195884 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2435 0.2923379835282253 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2419 0.29041707686027807 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2229 0.2676063101784042 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2170 0.26052296684034865 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2131 0.2558407568372272 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2103 0.25247917016831944 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2073 0.2488774701659183 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1961 0.23543112349028741 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1727 0.20733786347155858 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1631 0.19581242346387492 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1540 0.18488726678992484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1463 0.1756429034504286 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1191 0.142987490095325 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1134 0.13614426009076283 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1051 0.12617955675078638 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1047 0.12569933008379955 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1040 0.12485893341657262 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 958 0.11501428674334285 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 954 0.11453406007635603 No Hit AAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 937 0.11249309674166207 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 929 0.11153264340768843 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 929 0.11153264340768843 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 894 0.10733066007155376 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 885 0.10625015007083344 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.201983336134655E-4 0.0 12-13 0.0 0.0 0.0 6.002833337335222E-4 0.0 14-15 0.0 0.0 0.0 7.203400004802266E-4 0.0 16-17 0.0 0.0 0.0 8.403966672269311E-4 0.0 18-19 0.0 0.0 0.0 9.604533339736356E-4 0.0 20-21 0.0 0.0 0.0 0.0018008500012005667 0.0 22-23 0.0 0.0 0.0 0.0035416716690277812 0.0 24-25 0.0 0.0 0.0 0.00594280500396187 0.0 26-27 0.0 0.0 0.0 0.010745071673830047 0.0 28-29 0.0 0.0 0.0 0.020529690013686458 0.0 30-31 0.0 0.0 0.0 0.04003889836002593 0.0 32-33 0.0 0.0 0.0 0.07647609671765074 0.0 34-35 0.0 0.0 0.0 0.10919153840612769 0.0 36-37 0.0 6.002833337335222E-5 0.0 0.13950584675967057 0.0 38-39 0.0 1.2005666674670444E-4 0.0 0.1649578601099719 0.0 40-41 0.0 1.2005666674670444E-4 0.0 0.20751794847167865 0.0 42-43 0.0 1.2005666674670444E-4 0.0 0.2733090018488727 0.0 44-45 0.0 1.2005666674670444E-4 0.0 0.36491223857660815 0.0 46-47 0.0 1.2005666674670444E-4 0.0 0.5611448603740965 0.0 48-49 0.0 1.2005666674670444E-4 0.0 1.1573462674382307 0.0 50-51 0.0 1.2005666674670444E-4 0.0 1.4101856076067905 0.0 52-53 0.0 1.2005666674670444E-4 0.0 1.610500156073667 0.0 54-55 0.0 1.2005666674670444E-4 0.0 1.8557759262371838 0.0 56-57 0.0 1.2005666674670444E-4 0.0 1.9918601579945734 0.0 58-59 0.0 1.2005666674670444E-4 0.0 2.1798688981199126 0.0 60-61 0.0 1.2005666674670444E-4 0.0 2.6565538934377027 0.0 62-63 0.0 1.2005666674670444E-4 0.0 2.8874828919249884 0.0 64-65 0.0 1.2005666674670444E-4 0.0 3.0909189137272794 0.0 66-67 0.0 1.2005666674670444E-4 0.0 3.5063149806708767 0.0 68-69 0.0 1.2005666674670444E-4 0.0 3.8816121209210745 0.0 70-71 0.0 1.2005666674670444E-4 0.0 4.186435997790957 0.0 72-73 0.0 1.2005666674670444E-4 0.0 4.482795879655197 0.0 74-75 0.0 1.2005666674670444E-4 0.0 4.639469829759647 0.0 76-77 0.0 1.2005666674670444E-4 0.0 4.7306528681537685 0.0 78-79 0.0 1.2005666674670444E-4 0.0 4.896691238264461 0.0 80-81 0.0 1.2005666674670444E-4 0.0 5.1776238384517494 0.0 82-83 0.0 1.2005666674670444E-4 0.0 5.525187888683458 0.0 84-85 0.0 1.2005666674670444E-4 0.0 5.967656733978438 0.0 86-87 0.0 1.2005666674670444E-4 0.0 6.821199606214133 0.0 88-89 0.0 1.2005666674670444E-4 0.0 7.774029341849353 0.0 90-91 0.0 1.2005666674670444E-4 0.0 8.622709919081807 0.0 92-93 0.0 1.2005666674670444E-4 0.0 9.423908084615938 0.0 94-95 0.0 1.2005666674670444E-4 0.0 10.145568708430378 0.0 96-97 0.0 1.2005666674670444E-4 0.0 11.015559344010374 0.0 98-99 0.0 1.2005666674670444E-4 0.0 11.883929214589287 0.0 100-101 0.0 1.2005666674670444E-4 0.0 12.567711960045141 0.0 102-103 0.0 2.4011333349340888E-4 0.0 13.413331092275554 0.0 104-105 0.0 2.4011333349340888E-4 0.0 14.285242634523495 0.0 106-107 0.0 2.4011333349340888E-4 0.0 15.156914063437942 0.0 108-109 0.0 2.4011333349340888E-4 0.0 16.007335462338226 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 180 0.0 73.52418 2 ATAACGC 225 0.0 66.3907 3 TACCTGG 1145 0.0 60.304626 2 GTACCCG 100 1.8189894E-12 57.673676 1 ACCTGGG 1220 0.0 54.15698 3 GTACGCA 1045 0.0 51.68065 3 AGTACGC 1060 0.0 51.56946 2 GAGTACG 1075 0.0 51.50393 1 TACGCAG 1160 0.0 47.54483 4 GTACCTG 1570 0.0 45.918526 1 GTATAAC 325 0.0 44.36436 1 TACCCGG 130 2.5465852E-11 44.262047 2 ACGCAGT 1260 0.0 43.774086 5 CGCAGTG 1345 0.0 41.007698 6 GTAGGCA 820 0.0 37.13457 3 TAACGCC 95 9.5001815E-6 36.284214 4 TAACGCA 355 0.0 33.984512 4 TAGGCAG 885 0.0 33.755936 4 CCTGGGG 720 0.0 33.512505 4 CCTGGGA 810 0.0 33.33519 4 >>END_MODULE