##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003863_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 832940 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.499769491199846 31.0 31.0 34.0 30.0 34.0 2 31.66021322064014 31.0 31.0 34.0 30.0 34.0 3 31.736167070857444 31.0 31.0 34.0 30.0 34.0 4 35.39016375729344 37.0 35.0 37.0 33.0 37.0 5 35.31222537037482 37.0 35.0 37.0 33.0 37.0 6 35.39364299949576 37.0 35.0 37.0 33.0 37.0 7 35.339760366893174 37.0 35.0 37.0 32.0 37.0 8 35.363313083775544 37.0 35.0 37.0 32.0 37.0 9 36.86933992844622 39.0 37.0 39.0 33.0 39.0 10-11 36.74707842101472 39.0 36.0 39.0 32.0 39.0 12-13 36.728978677935984 39.0 35.0 39.0 32.0 39.0 14-15 37.59455362931303 39.0 36.0 41.0 32.0 41.0 16-17 37.739325761759545 39.0 37.0 41.0 32.0 41.0 18-19 37.82282697433188 40.0 37.0 41.0 32.0 41.0 20-21 37.70552020553701 39.5 37.0 41.0 32.0 41.0 22-23 37.58873568324249 39.0 36.5 41.0 32.0 41.0 24-25 37.36868802074579 39.0 36.0 41.0 31.5 41.0 26-27 37.02749717866833 39.0 36.0 40.5 30.5 41.0 28-29 36.91981595312988 39.0 36.0 40.0 31.0 41.0 30-31 36.71860698249574 38.5 35.5 40.0 30.0 41.0 32-33 36.35517984488679 38.0 35.0 40.0 30.0 41.0 34-35 36.11991319902994 38.0 35.0 40.0 30.0 41.0 36-37 35.80212920498475 38.0 34.5 40.0 28.5 41.0 38-39 35.55683482603789 38.0 34.5 40.0 28.5 41.0 40-41 35.91028585492353 38.0 35.0 40.0 30.0 41.0 42-43 35.74611916824741 38.0 35.0 40.0 28.5 41.0 44-45 35.55392645328595 38.0 35.0 40.0 27.0 41.0 46-47 35.27002365116335 38.0 34.5 40.0 26.5 41.0 48-49 34.93723077292482 38.0 34.0 40.0 25.5 41.0 50-51 34.55064110260042 38.0 33.0 40.0 24.0 41.0 52-53 34.50533171657022 38.0 34.0 40.0 24.0 41.0 54-55 34.2950422599467 38.0 33.0 40.0 23.0 41.0 56-57 33.83618627992412 37.5 32.5 40.0 22.5 41.0 58-59 33.469944413763294 37.0 32.0 40.0 21.5 41.0 60-61 32.73974235839316 36.0 31.0 39.5 18.5 41.0 62-63 32.68731301174154 36.0 31.0 39.0 19.0 41.0 64-65 32.258030590438686 36.0 31.0 39.0 16.0 40.5 66-67 31.712553125075033 35.5 30.5 39.0 13.0 40.0 68-69 31.282896727255263 35.0 30.0 38.0 11.0 40.0 70-71 30.651462890484307 34.5 29.5 37.0 7.5 39.0 72-73 30.13663709270776 34.0 29.0 36.5 7.0 39.0 74-75 29.815055106010036 34.0 29.0 36.0 4.0 38.5 76-77 28.89641750906428 33.5 28.5 35.0 2.0 37.0 78-79 29.226953922251305 34.0 29.0 35.0 2.0 37.0 80-81 28.80576272000384 34.0 28.5 35.0 2.0 37.0 82-83 28.365797056210532 33.5 27.5 35.0 2.0 36.0 84-85 28.02812747616875 33.0 26.5 35.0 2.0 36.0 86-87 27.917447835378297 33.5 27.0 35.0 2.0 36.0 88-89 27.54121485341081 33.0 26.5 35.0 2.0 35.0 90-91 27.108259298388838 33.0 25.0 35.0 2.0 35.0 92-93 26.793178980478785 33.0 25.0 35.0 2.0 35.0 94-95 26.367749177611834 33.0 25.0 35.0 2.0 35.0 96-97 25.85014166686676 32.5 24.0 35.0 2.0 35.0 98-99 25.536279924124187 33.0 21.5 35.0 2.0 35.0 100-101 24.42869654476913 32.0 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.08322805151352952 1101 2 0.23015025794592248 1101 3 0.14138290811769139 1101 4 0.21409916543792917 1101 5 0.21708387479016267 1101 6 0.1366592277825589 1101 7 0.05392779946309645 1101 8 0.04075516512049404 1101 9 0.28164691652571605 1101 10-11 0.3823857698269677 1101 12-13 0.38904285241665804 1101 14-15 0.34262520735307334 1101 16-17 0.28547115330730577 1101 18-19 0.23149746860490694 1101 20-21 0.27345729900046933 1101 22-23 0.20949024399681804 1101 24-25 0.287134296017463 1101 26-27 0.5342650649949832 1101 28-29 0.45431760253596565 1101 30-31 0.3496861227968182 1101 32-33 0.3951686579897 1101 34-35 0.4439257099351437 1101 36-37 0.4122106192781274 1101 38-39 0.17103550139784574 1101 40-41 0.4199289884777855 1101 42-43 0.26509699661024655 1101 44-45 0.26005645008918776 1101 46-47 0.26255160750894646 1101 48-49 0.25282363508304684 1101 50-51 0.5193241187633433 1101 52-53 0.5036155669326803 1101 54-55 0.29705782541846304 1101 56-57 0.42058682709830464 1101 58-59 0.319414895856724 1101 60-61 0.37931809231788094 1101 62-63 0.3568552673794727 1101 64-65 0.4995681880059877 1101 66-67 0.4891882918961379 1101 68-69 0.1190597127345967 1101 70-71 0.36812265926623766 1101 72-73 0.41919534627207966 1101 74-75 0.41889538300755547 1101 76-77 0.5191251418516778 1101 78-79 0.5183608929511614 1101 80-81 0.2220763463038402 1101 82-83 0.3126680901116181 1101 84-85 0.1973519318858621 1101 86-87 0.08461390961119974 1101 88-89 0.060214424419914536 1101 90-91 0.3512736358835298 1101 92-93 0.4251685554978799 1101 94-95 0.2795820077495357 1101 96-97 0.4355725729248121 1101 98-99 0.44661591297643 1101 100-101 0.4973024154612453 1102 1 0.046458984350799426 1102 2 0.032968327436048384 1102 3 0.016105631033770607 1102 4 -0.04142931707301045 1102 5 0.012533433718822096 1102 6 -0.045167352394997806 1102 7 -0.03330700921702601 1102 8 0.025513192639813553 1102 9 0.07562361317802413 1102 10-11 0.039082359148238766 1102 12-13 0.01483767839739869 1102 14-15 0.05346916898516696 1102 16-17 0.04623916227675551 1102 18-19 -0.12260623223713907 1102 20-21 0.015517962449862921 1102 22-23 0.0018549805455378987 1102 24-25 -0.04013822129628153 1102 26-27 -0.07670350729441822 1102 28-29 0.21415969238371702 1102 30-31 0.326060488064563 1102 32-33 -0.02088186654032853 1102 34-35 -0.1861129120200573 1102 36-37 0.12114867822831599 1102 38-39 -0.06345614065988059 1102 40-41 -0.015368476162500144 1102 42-43 0.1551566984474917 1102 44-45 0.029063774508365725 1102 46-47 -0.18598167151498757 1102 48-49 -0.28732524683287153 1102 50-51 -0.13727169024537034 1102 52-53 -0.31985984214615826 1102 54-55 -0.5591895566554399 1102 56-57 -0.29306586084910435 1102 58-59 -0.5445070119985758 1102 60-61 -0.4351731718136733 1102 62-63 -0.5546916369327661 1102 64-65 -0.46246282923479853 1102 66-67 -0.6869515491859026 1102 68-69 -0.41205830835998114 1102 70-71 -0.4045295531240747 1102 72-73 -0.3003860585004965 1102 74-75 -0.14475630763467606 1102 76-77 -0.04807748011612034 1102 78-79 -0.1555659804341225 1102 80-81 0.019585077973108156 1102 82-83 -0.1412002467087845 1102 84-85 -0.2963183296285514 1102 86-87 -0.5367765017719357 1102 88-89 -0.43529612759374814 1102 90-91 -0.4180271062164991 1102 92-93 -0.4078983048909457 1102 94-95 -0.41933470886987223 1102 96-97 -0.28488331101825537 1102 98-99 -0.3552352953900133 1102 100-101 -0.3693185151399625 1103 1 -0.0417275788929139 1103 2 -0.13800501989185676 1103 3 -0.25252464464549007 1103 4 -0.08733726962397981 1103 5 -0.047713859565419625 1103 6 0.07345413116811272 1103 7 0.007626025439201101 1103 8 -0.054100784312822725 1103 9 -0.12705050075688007 1103 10-11 0.013044589091023795 1103 12-13 0.07344559698130837 1103 14-15 0.08133307794908973 1103 16-17 -0.1605727071892673 1103 18-19 -0.13648521558453552 1103 20-21 -0.1372846474433942 1103 22-23 -0.058505900326203175 1103 24-25 -0.0013131101388026423 1103 26-27 0.141783309446204 1103 28-29 -0.14337962912703972 1103 30-31 -0.013143175572004395 1103 32-33 -0.022481376366208394 1103 34-35 0.05372946335852191 1103 36-37 0.05625521574007308 1103 38-39 0.15011910510980186 1103 40-41 0.07106457869921456 1103 42-43 0.17318545139776376 1103 44-45 0.022946427608594888 1103 46-47 0.16353115787536154 1103 48-49 -0.06890232311021549 1103 50-51 0.1443731017091352 1103 52-53 0.15005910655703758 1103 54-55 -0.08372338336666019 1103 56-57 0.11761224612637733 1103 58-59 0.17300175008239194 1103 60-61 0.19334572249854176 1103 62-63 0.11056847318166518 1103 64-65 -0.0759073400600272 1103 66-67 -0.1841369798303063 1103 68-69 -0.17168731382420077 1103 70-71 -0.1292281910559936 1103 72-73 -0.05703612026374927 1103 74-75 0.021541927498180513 1103 76-77 0.157497486053348 1103 78-79 0.20579053932957336 1103 80-81 0.26530834147555993 1103 82-83 0.20368334239685382 1103 84-85 0.04397533797266462 1103 86-87 0.14159423719177155 1103 88-89 0.08536370707077978 1103 90-91 0.11161270099041332 1103 92-93 0.09169103229271514 1103 94-95 0.25536190851361695 1103 96-97 0.35442913457711 1103 98-99 0.24299448574636173 1103 100-101 0.4097285068446972 1104 1 -0.07689971785000083 1104 2 -0.029863919082007584 1104 3 0.05991122695112594 1104 4 0.04346520573238877 1104 5 0.019094194962043787 1104 6 -0.013983801698614684 1104 7 0.006540423181881749 1104 8 0.08110135757584658 1104 9 0.18421617262799828 1104 10-11 -7.401547618144377E-4 1104 12-13 -0.16118763435732575 1104 14-15 0.1252598891184178 1104 16-17 0.022197250485994857 1104 18-19 -0.05607220576373351 1104 20-21 -0.09403977313506573 1104 22-23 0.12759644402499504 1104 24-25 -0.032054663511964065 1104 26-27 0.017276296547230174 1104 28-29 -0.02679877237157058 1104 30-31 -0.011731778122488379 1104 32-33 -0.09669110830947858 1104 34-35 0.26748775424496074 1104 36-37 0.15011171469569007 1104 38-39 0.23476058152886736 1104 40-41 0.15308881465509216 1104 42-43 0.18426592338281012 1104 44-45 0.2978528688486577 1104 46-47 0.39068255129395624 1104 48-49 0.3931239283097696 1104 50-51 0.6113223532390606 1104 52-53 0.39660762427480023 1104 54-55 0.17896087127807192 1104 56-57 0.2710560559032089 1104 58-59 0.16066905650622232 1104 60-61 0.45852067852477774 1104 62-63 0.7742058362217179 1104 64-65 0.8939889551504976 1104 66-67 0.6640109689769069 1104 68-69 0.8255666163308035 1104 70-71 0.8075406306303989 1104 72-73 0.7984539581561272 1104 74-75 0.7592172499724406 1104 76-77 0.7418629813078432 1104 78-79 0.8928628257819895 1104 80-81 0.8228801881903536 1104 82-83 0.8787046421879801 1104 84-85 0.8612529040249193 1104 86-87 0.68083223143854 1104 88-89 0.7217230230955529 1104 90-91 0.9361316234500094 1104 92-93 0.7108587830347766 1104 94-95 0.6396135758521062 1104 96-97 0.7973393960377706 1104 98-99 0.5250529616890525 1104 100-101 0.5143946497960705 1105 1 -0.06811018980296524 1105 2 -0.011639304430808295 1105 3 1.92094581827007E-4 1105 4 0.1085226107856272 1105 5 -0.08822743224278184 1105 6 0.07333047607033905 1105 7 -0.10709774621268053 1105 8 0.09275617244951917 1105 9 0.15258876916383457 1105 10-11 0.10429470540027808 1105 12-13 0.006980369774254314 1105 14-15 0.12349701767396226 1105 16-17 0.09878284161899131 1105 18-19 -0.036121963430979065 1105 20-21 0.12681325801065668 1105 22-23 0.25524075119168543 1105 24-25 0.18912859566490425 1105 26-27 0.06423074990811273 1105 28-29 0.2167456420571554 1105 30-31 0.3418828532139173 1105 32-33 0.23316696812465665 1105 34-35 0.4588909005980497 1105 36-37 0.2246985572191349 1105 38-39 0.33409218013049014 1105 40-41 0.40371699278952633 1105 42-43 0.6438638893767674 1105 44-45 0.3816190574709353 1105 46-47 0.5029625648010239 1105 48-49 0.350807564890097 1105 50-51 0.6601042339001069 1105 52-53 0.700367754578302 1105 54-55 0.4797986076518157 1105 56-57 0.5085992586681911 1105 58-59 0.616907635085596 1105 60-61 0.4760922858662653 1105 62-63 0.3642860841162019 1105 64-65 0.5289007619199282 1105 66-67 0.46372146079320586 1105 68-69 0.5699353593786363 1105 70-71 0.6727008284692708 1105 72-73 0.4646157649255578 1105 74-75 0.5496416302362235 1105 76-77 0.3812440396266119 1105 78-79 0.4011135607255767 1105 80-81 0.7183965357193536 1105 82-83 0.7753904438723929 1105 84-85 0.5080529199155954 1105 86-87 0.5634870661755471 1105 88-89 0.5844797567678874 1105 90-91 0.7890063453632479 1105 92-93 0.6686938775842712 1105 94-95 0.6720355129250422 1105 96-97 1.0026046709464005 1105 98-99 0.8760828758794226 1105 100-101 0.5861733125960065 1106 1 -0.12782170640729262 1106 2 -0.17505295572960478 1106 3 -0.14670505571471537 1106 4 -0.11675688333518508 1106 5 -0.1135213918633724 1106 6 -0.2000003085797033 1106 7 -0.16720706406104568 1106 8 -0.0842912123947741 1106 9 -0.31048287639008976 1106 10-11 -0.23111890456851825 1106 12-13 -0.22779985795404656 1106 14-15 -0.13978282775184425 1106 16-17 -0.10741510841303636 1106 18-19 -0.19801157100264533 1106 20-21 -0.14009329486074762 1106 22-23 -0.4067340995036872 1106 24-25 -0.3258974422347123 1106 26-27 -0.21752877149049255 1106 28-29 -0.10568001560645968 1106 30-31 -0.0318410830552196 1106 32-33 -0.017778455611164645 1106 34-35 0.015226272878017255 1106 36-37 -0.2542102274722353 1106 38-39 -0.26318572278534447 1106 40-41 -0.22961616833547538 1106 42-43 -0.1360719457991877 1106 44-45 -0.12050427124722063 1106 46-47 -0.4578875932862232 1106 48-49 -0.1655513654895131 1106 50-51 0.12769634813622588 1106 52-53 0.2174577093219554 1106 54-55 -0.11198498847142702 1106 56-57 0.16546468819020532 1106 58-59 -0.12497102003621308 1106 60-61 -0.047811952525172785 1106 62-63 -0.3155769069358527 1106 64-65 -0.18408047426035523 1106 66-67 -0.3326378828864023 1106 68-69 -0.2514880458000128 1106 70-71 -0.17107435699910312 1106 72-73 0.004997757444826334 1106 74-75 0.06936637244035282 1106 76-77 -0.030345925449537958 1106 78-79 0.1199894880674357 1106 80-81 0.07398435163553785 1106 82-83 0.1569489981490122 1106 84-85 0.18564017787678466 1106 86-87 0.3515571618544975 1106 88-89 0.3540365958495322 1106 90-91 0.09630780637872505 1106 92-93 -0.12003749808475561 1106 94-95 -0.03899029765956996 1106 96-97 0.3427453995942926 1106 98-99 0.23762737795625455 1106 100-101 -0.006589287887553752 1107 1 -0.08334550811276387 1107 2 0.016173917262978676 1107 3 -0.07332971836569158 1107 4 -0.10627982306899497 1107 5 0.0912456192499036 1107 6 0.0885447037854874 1107 7 0.1249910662813889 1107 8 0.10135849874210834 1107 9 0.14942517996643545 1107 10-11 0.14972120995057026 1107 12-13 0.04633278306554445 1107 14-15 0.17688785802869944 1107 16-17 0.14957558434537788 1107 18-19 0.032125882692035646 1107 20-21 -0.03982880296226199 1107 22-23 0.25152748837124506 1107 24-25 0.13252012146396908 1107 26-27 0.2665106431897044 1107 28-29 0.4880260520583164 1107 30-31 0.27727708915440274 1107 32-33 0.3301797490250138 1107 34-35 0.3140828777358493 1107 36-37 0.33033739133105655 1107 38-39 0.5419663536428132 1107 40-41 0.5182956027084344 1107 42-43 0.580590787266658 1107 44-45 0.5795898786332359 1107 46-47 0.3685007967764804 1107 48-49 0.1940306295644092 1107 50-51 0.5302678969145873 1107 52-53 0.7359067247245719 1107 54-55 0.5856707564670529 1107 56-57 0.47182121608627625 1107 58-59 0.30032990042636953 1107 60-61 0.30265905341627786 1107 62-63 0.28406932552969977 1107 64-65 0.12773392477095769 1107 66-67 0.3602167312273572 1107 68-69 0.5843204630916361 1107 70-71 0.44439777771475164 1107 72-73 0.7207715822388252 1107 74-75 0.780360872240248 1107 76-77 0.6555179959058073 1107 78-79 0.5555006680450667 1107 80-81 0.5096391935560902 1107 82-83 0.4485455441054427 1107 84-85 0.44338124774779786 1107 86-87 0.15243401278470614 1107 88-89 0.21032206239169327 1107 90-91 0.22196198129740452 1107 92-93 0.2552074968357694 1107 94-95 0.1608126802472718 1107 96-97 0.4174803946174279 1107 98-99 0.3659277545557771 1107 100-101 0.5257487589257188 1108 1 -0.21340068465631745 1108 2 -0.1782485468615782 1108 3 -0.26439186682908655 1108 4 -0.13157709204395474 1108 5 -0.055188757019905665 1108 6 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15.0 5 25.0 6 65.0 7 232.0 8 514.0 9 893.0 10 1594.0 11 2784.0 12 4424.0 13 5952.0 14 5767.0 15 6172.0 16 7229.0 17 8964.0 18 8507.0 19 7117.0 20 7045.0 21 7235.0 22 7821.0 23 8417.0 24 9642.0 25 10698.0 26 12947.0 27 15887.0 28 19300.0 29 23370.0 30 28022.0 31 33647.0 32 39785.0 33 49170.0 34 61529.0 35 80115.0 36 109598.0 37 132683.0 38 100059.0 39 15697.0 40 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.70032155422278 17.609087600403114 11.625800732475776 19.064790112898326 2 13.920825649300753 27.832766635930533 33.8686942907638 24.377713424004916 3 29.17826202363952 24.89235385045923 28.56499425286666 17.364389873034593 4 11.463730880975822 16.356160107570776 43.099022738732685 29.081086272720725 5 10.647465603764978 42.912094508608064 31.97733330131822 14.463106586308738 6 24.56946645536186 37.82019881861403 17.578278826297844 20.032055899726263 7 27.107354671404906 31.919826157946552 23.976156745984103 16.99666242466444 8 22.871995581914664 26.358441184238963 19.72999255648666 31.039570677359713 9 20.651667587101112 24.514610896343072 27.17950872811967 27.654212788436144 10-11 26.41948095683164 22.9134513855498 28.174476685797778 22.492590971820782 12-13 33.38589814392393 21.346735659231157 25.760138785506758 19.507227411338153 14-15 23.681735095402033 25.81176520024372 31.72017804777755 18.786321656576693 16-17 19.096633611064423 28.021346075347566 31.58402766105602 21.297992652531995 18-19 17.9635988186424 34.64703339976469 27.604809470069874 19.78455831152304 20-21 22.13504394601604 26.966464146774893 25.755367177369003 25.143124729840068 22-23 18.582190794054792 28.298676975532448 27.278375393185584 25.84075683722717 24-25 24.92046245828031 27.444773933296517 26.572562249381708 21.06220135904147 26-27 23.659360344632997 22.539579246139326 34.6767144603223 19.124345948905372 28-29 18.56501606813634 33.410497208712876 28.483075476716063 19.541411246434723 30-31 21.838268268947335 25.93760374711309 27.69211188749055 24.53201609644902 32-33 23.04042368114416 27.17067849855413 29.748928580647753 20.039969239653953 34-35 26.183006394694058 23.62602926238897 30.17740893624276 20.013555406674207 36-37 24.204409818703184 23.28417657526976 33.684187142311636 18.827226463715423 38-39 21.08372941044395 28.879979780406085 32.034428541325596 18.00186226782437 40-41 20.537679919552136 30.164527894096988 28.404040404040405 20.893751782310474 42-43 18.801234182534156 30.190409873460272 25.331176315220784 25.677179628784785 44-45 22.392969481595312 25.714937450476626 25.468581170312387 26.423511897615676 46-47 25.61740826892774 29.59279657837473 26.124709236887522 18.66508591581001 48-49 23.77194035584796 27.651991740101327 28.290092923860062 20.285974980190648 50-51 17.80224265853483 29.09699378106466 33.23072490215382 19.870038658246692 52-53 21.374108579249405 30.934641150623094 26.722152856148103 20.969097413979398 54-55 18.732621797488413 27.567171705044778 28.062705597041806 25.637500900425003 56-57 24.26040404792291 24.556650630732086 28.66568378033451 22.51726154101049 58-59 29.384370467839066 24.348389312023976 26.419496816081907 19.847743404055056 60-61 22.51023282452372 27.596868137343776 32.06389076662436 17.829008271508144 62-63 19.593571933313044 28.067887703953993 31.373657942656035 20.964882420076933 64-65 18.608185975563984 34.7100737764726 26.23397681310861 20.447763434854803 66-67 21.673146260698836 27.399346136410713 24.689504858944993 26.23800274394546 68-69 19.2696105474019 29.22182115927535 25.860115940741395 25.64845235258135 70-71 25.145179047154826 28.223503098073444 24.593036448134733 22.038281406636997 72-73 23.419393953946262 23.741085792494058 31.99119984632747 20.84832040723221 74-75 18.70892183645991 33.85389935283415 26.34044849595919 21.09673031474675 76-77 21.879006891252672 26.586668907724448 25.803779383869184 25.730544817153696 78-79 23.175859005450572 28.1093476120729 27.51248589334166 21.20230748913487 80-81 25.890235725403826 25.495849338397324 27.25089877476095 21.363016161437894 82-83 23.637717002425145 24.67878838811919 31.16881167911254 20.514682930343124 84-85 20.64321598387398 29.35243195934399 29.40915876843396 20.595193288348074 86-87 20.918871772030094 30.152971212022074 26.047306240766833 22.880850775181003 88-89 19.534540303023025 29.859953898239972 23.901961725934644 26.703544072802366 90-91 22.073318606382212 25.439167286959446 24.720388023146924 27.767126083511418 92-93 24.29178572286119 29.026460489350974 25.822448195548297 20.859305592239537 94-95 22.93443881578434 27.52768958609839 27.286375541080716 22.251496057036555 96-97 17.142522698281685 29.233405867787198 32.06128911232835 21.56278232160276 98-99 20.974133051118603 30.195975558731735 26.04925591477371 22.780635475375945 100-101 18.797884339828666 27.26280612511353 26.612692640391195 27.326616894666607 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 250.0 1 187.0 2 481.5 3 745.5 4 515.5 5 385.5 6 468.0 7 663.5 8 873.0 9 1025.0 10 1031.0 11 970.0 12 1029.5 13 1104.0 14 1207.5 15 1296.0 16 1344.5 17 1478.0 18 1584.5 19 1647.0 20 1747.0 21 1901.0 22 2085.0 23 2384.5 24 2787.5 25 3188.5 26 3706.0 27 4562.0 28 5598.0 29 6735.5 30 7895.5 31 8951.5 32 10030.0 33 11129.5 34 12221.5 35 13433.5 36 14586.5 37 15581.0 38 16611.5 39 17536.0 40 18375.5 41 19021.5 42 19662.5 43 20858.0 44 21739.5 45 23208.5 46 27854.5 47 53250.0 48 116281.0 49 122357.0 50 65482.5 51 34758.0 52 22584.5 53 16976.0 54 13637.0 55 11655.0 56 10005.0 57 8231.0 58 6658.0 59 5376.0 60 4322.5 61 3288.0 62 2367.5 63 1660.0 64 1026.5 65 627.5 66 382.5 67 214.0 68 115.0 69 50.5 70 26.0 71 22.5 72 12.5 73 6.0 74 6.0 75 4.0 76 2.0 77 1.0 78 0.5 79 1.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.050543856700362565 2 0.005282493336854995 3 0.04213989002809326 4 0.0 5 0.0 6 0.002401133334934089 7 0.0 8 0.0 9 0.0 10-11 4.2019833361346557E-4 12-13 0.0 14-15 0.002100991668067328 16-17 0.0 18-19 0.0 20-21 0.012005666674670444 22-23 0.0 24-25 0.0 26-27 0.00648306000432204 28-29 0.009664561673109707 30-31 0.010264845006843229 32-33 0.0059428050039618706 34-35 0.007443513338295675 36-37 0.01944918001296612 38-39 0.010144788340096526 40-41 0.012305808341537204 42-43 0.0 44-45 0.0 46-47 3.001416668667611E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0022810766681873843 58-59 4.802266669868178E-4 60-61 0.00972459000648306 62-63 0.014826998343217998 64-65 0.04292025836194684 66-67 0.02371119168247413 68-69 0.0017408216678272144 70-71 3.601700002401133E-4 72-73 0.0 74-75 6.002833337335223E-5 76-77 0.0 78-79 0.0 80-81 6.002833337335223E-5 82-83 0.0 84-85 6.002833337335223E-5 86-87 6.603116671068744E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.002833337335223E-5 96-97 3.001416668667611E-4 98-99 5.4025500036017E-4 100-101 0.0010204816673469877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 832940.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.38803629726737 #Duplication Level Percentage of deduplicated Percentage of total 1 83.7169866853159 39.67183603741599 2 10.402391655091984 9.858978266597802 3 2.408748744575245 3.424376188167868 4 1.0261185963230204 1.9450298115142535 5 0.5608708602490012 1.3289284341779624 6 0.368423058112963 1.0475307150364401 7 0.26314094669805993 0.8728812913397475 8 0.18720266744254335 0.7096933439770015 9 0.14812965123105612 0.631761596031496 >10 0.8181132486595105 7.037466906174444 >50 0.053992769017650266 1.7384137143248664 >100 0.029911446626531536 2.8781335130353214 >500 0.00532322355216321 1.7561899816573796 >1k 0.007858091910336169 8.330490781664794 >5k 0.0020278946865383662 7.443026241002365 >10k+ 7.604605074518873E-4 11.325263177882313 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 47968 5.758878190505919 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 29198 3.505414555670276 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 17115 2.0547698513698465 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9868 1.1847191874564793 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 9385 1.1267318174178211 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8273 0.9932288039954859 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7931 0.952169423968113 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7766 0.9323600739549067 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7016 0.8423175738948784 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6033 0.724301870482868 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5690 0.6831224337887483 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4876 0.5853963070569308 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4731 0.5679880903786587 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4586 0.5505798737003866 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3707 0.4450500636300334 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3598 0.4319638869546426 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3584 0.43028309362018874 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2943 0.3533267702355512 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2935 0.35236631690157755 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2818 0.3383196868922131 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2559 0.3072250102048167 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2446 0.29365860686243905 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2222 0.2667659135111773 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2088 0.2506783201671189 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1977 0.2373520301582347 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1947 0.23375033015583355 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1894 0.2273873268182582 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1865 0.22390568348260378 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1824 0.21898336014598893 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1632 0.19593248013062164 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1545 0.18548755012365836 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1510 0.1812855667875237 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1474 0.17696352678464236 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1465 0.175883016783922 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1341 0.16099599010733068 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1229 0.14754964343169977 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1221 0.14658919009772614 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1171 0.1405863567603909 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1085 0.13026148342017432 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1060 0.12726006675150672 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1013 0.12161740341441162 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1004 0.12053689341369127 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 942 0.1130933800753956 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 903 0.10841117007227412 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 901 0.10817105673878072 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 894 0.10733066007155376 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 843 0.10120777006747185 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.001416668667611E-4 0.0 12-13 0.0 0.0 0.0 4.8022666698681777E-4 0.0 14-15 0.0 0.0 0.0 6.002833337335222E-4 0.0 16-17 0.0 0.0 0.0 7.203400004802266E-4 0.0 18-19 0.0 0.0 0.0 7.803683338535789E-4 0.0 20-21 0.0 0.0 0.0 0.0015007083343338056 0.0 22-23 0.0 0.0 0.0 0.003361586668907724 0.0 24-25 0.0 0.0 0.0 0.005822748337215166 0.0 26-27 0.0 6.002833337335222E-5 0.0 0.010805100007203399 0.0 28-29 0.0 1.2005666674670444E-4 0.0 0.02088986001392657 0.0 30-31 0.0 1.2005666674670444E-4 0.0 0.04177972002785314 0.0 32-33 0.0 2.4011333349340888E-4 0.0 0.08031791005354527 0.0 34-35 0.0 2.4011333349340888E-4 0.0 0.11603476841068985 0.0 36-37 0.0 2.4011333349340888E-4 0.0 0.1485100967656734 0.0 38-39 0.0 2.4011333349340888E-4 0.0 0.17606310178404205 0.0 40-41 0.0 2.4011333349340888E-4 0.0 0.2212644368141763 0.0 42-43 0.0 2.4011333349340888E-4 0.0 0.2901769635267846 0.0 44-45 0.0 2.4011333349340888E-4 0.0 0.38742286359161526 0.0 46-47 0.0 2.4011333349340888E-4 0.0 0.5944605853963071 0.0 48-49 0.0 2.4011333349340888E-4 0.0 1.2090906908060606 0.0 50-51 0.0 2.4011333349340888E-4 0.0 1.4691934593127955 0.0 52-53 0.0 2.4011333349340888E-4 0.0 1.6767714361178476 0.0 54-55 0.0 2.4011333349340888E-4 0.0 1.9275698129517131 0.0 56-57 0.0 2.4011333349340888E-4 0.0 2.068276226378851 0.0 58-59 0.0 2.4011333349340888E-4 0.0 2.262407856508272 0.0 60-61 0.0 2.4011333349340888E-4 0.0 2.750138065166759 0.0 62-63 0.0 2.4011333349340888E-4 0.0 2.9831680553221123 0.0 64-65 0.0 2.4011333349340888E-4 0.0 3.1910461737940308 0.0 66-67 0.0 2.4011333349340888E-4 0.0 3.614606094076404 0.0 68-69 0.0 2.4011333349340888E-4 0.0 3.999927965999952 0.0 70-71 0.0 2.4011333349340888E-4 0.0 4.313215837875477 0.0 72-73 0.0 2.4011333349340888E-4 0.0 4.61876005474584 0.0 74-75 0.0 2.4011333349340888E-4 0.0 4.783537829855692 0.0 76-77 0.0 2.4011333349340888E-4 0.0 4.880303503253536 0.0 78-79 0.0 2.4011333349340888E-4 0.0 5.053605301702404 0.0 80-81 0.0 2.4011333349340888E-4 0.0 5.347564050231709 0.0 82-83 0.0 2.4011333349340888E-4 0.0 5.706893653804595 0.0 84-85 0.0 2.4011333349340888E-4 0.0 6.1642495257761665 0.0 86-87 0.0 2.4011333349340888E-4 0.0 7.0469061396979376 0.0 88-89 0.0 2.4011333349340888E-4 0.0 8.032451317021636 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 180 0.0 52.785152 2 TACCTGG 1255 0.0 52.61691 2 TACGCAG 1175 0.0 51.730164 4 AGTACGC 1185 0.0 50.91428 2 GAGTACG 1235 0.0 48.882336 1 ACGCAGT 1230 0.0 48.644886 5 GTACGCA 1250 0.0 47.886684 3 CGCAGTG 1250 0.0 47.866566 6 ACCTGGG 1305 0.0 47.324615 3 GTACACG 105 2.0008883E-11 45.271603 1 GTACCTG 1515 0.0 43.299377 1 GTACCCG 100 6.311893E-10 42.781666 1 GTAGGCA 835 0.0 42.10169 3 TACCCGG 105 1.024091E-9 40.719975 2 GTATAAC 260 0.0 40.22208 1 TACACGG 110 1.6170816E-9 38.86907 2 ATAACGC 270 0.0 38.709114 3 CCTGGGG 745 0.0 38.2443 4 GTACCGG 50 0.001609694 38.028145 1 CCGATAC 50 0.0016144521 38.005306 3 >>END_MODULE