##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003862_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 874633 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.70853489406414 31.0 31.0 34.0 27.0 34.0 2 30.60228232870244 31.0 31.0 34.0 27.0 34.0 3 30.69649327203524 31.0 31.0 34.0 27.0 34.0 4 34.2129967655005 37.0 35.0 37.0 30.0 37.0 5 34.206514046462914 37.0 35.0 37.0 30.0 37.0 6 34.31309246278153 37.0 35.0 37.0 30.0 37.0 7 34.297694004228056 37.0 35.0 37.0 30.0 37.0 8 34.280465063632406 37.0 35.0 37.0 30.0 37.0 9 35.70471500617973 39.0 35.0 39.0 30.0 39.0 10-11 35.555619328335425 38.5 35.0 39.0 30.0 39.0 12-13 35.43517738297092 38.0 35.0 39.0 30.0 39.0 14-15 36.34706328254251 39.0 36.0 41.0 29.0 41.0 16-17 36.394044702177936 39.0 36.0 41.0 30.0 41.0 18-19 36.342431625607546 39.0 36.0 41.0 29.5 41.0 20-21 36.2594671136351 39.0 36.0 41.0 29.0 41.0 22-23 36.15915418238278 39.0 35.5 41.0 29.0 41.0 24-25 35.898486565222214 39.0 35.0 40.5 27.0 41.0 26-27 35.734953403313156 39.0 35.0 40.0 27.0 41.0 28-29 35.563143627098455 38.0 35.0 40.0 27.0 41.0 30-31 35.27592601696941 38.0 34.0 40.0 26.0 41.0 32-33 34.991830859343295 38.0 34.0 40.0 25.0 41.0 34-35 34.94008629905343 38.0 34.0 40.0 25.0 41.0 36-37 34.740591196536144 38.0 34.0 40.0 24.5 41.0 38-39 34.69190048854777 38.0 34.0 40.0 24.5 41.0 40-41 34.842814643399 38.0 34.0 40.0 24.0 41.0 42-43 34.69578783329693 38.0 34.0 40.0 24.0 41.0 44-45 34.45666811108202 38.0 33.5 40.0 23.5 41.0 46-47 34.1561695019511 38.0 33.0 40.0 21.5 41.0 48-49 33.85085401534129 38.0 33.0 40.0 19.0 41.0 50-51 32.97468766899945 37.5 32.0 39.5 15.0 40.5 52-53 33.09136574997742 37.5 32.5 39.5 15.5 40.5 54-55 33.40397057966027 38.0 33.0 40.0 15.5 41.0 56-57 33.15032076310864 38.0 32.5 40.0 13.0 41.0 58-59 32.862303388964285 38.0 32.0 40.0 10.0 41.0 60-61 32.495171117485846 37.0 31.0 40.0 8.0 41.0 62-63 32.243738230777936 36.5 31.0 40.0 8.0 41.0 64-65 31.92656119766805 36.0 31.0 39.0 7.0 41.0 66-67 31.50213232292859 36.0 30.0 39.0 2.0 41.0 68-69 31.09581161470011 35.0 30.0 39.0 2.0 40.0 70-71 30.57580665261887 35.0 29.0 38.0 2.0 40.0 72-73 30.158798033003556 35.0 29.0 37.0 2.0 40.0 74-75 29.78906810056332 34.5 29.0 37.0 2.0 39.0 76-77 29.352335208024392 34.0 29.0 36.5 2.0 39.0 78-79 28.80505366250759 34.0 27.5 36.0 2.0 38.5 80-81 28.32345852488987 34.0 26.0 35.5 2.0 37.5 82-83 27.916158548785603 34.0 26.0 35.0 2.0 37.0 84-85 27.62673372717471 34.0 26.0 35.0 2.0 36.5 86-87 27.437822492405388 34.0 26.0 35.0 2.0 36.0 88-89 27.081535341108783 33.5 25.0 35.0 2.0 36.0 90-91 26.69919440496757 33.0 25.0 35.0 2.0 35.0 92-93 26.43623954275679 33.0 24.0 35.0 2.0 35.0 94-95 26.157623254553624 33.0 24.0 35.0 2.0 35.0 96-97 25.79305377226791 32.5 21.0 35.0 2.0 35.0 98-99 25.502220359853787 32.0 19.5 35.0 2.0 35.0 100-101 25.1600093982276 32.0 19.0 35.0 2.0 35.0 102-103 24.717487220354137 32.0 17.0 35.0 2.0 35.0 104-105 24.25295981285865 31.5 9.5 34.0 2.0 35.0 106-107 23.795011164682787 31.0 7.0 34.0 2.0 35.0 108-109 23.421832357114354 31.0 2.0 34.0 2.0 35.0 110-111 22.995981171531376 31.0 2.0 34.0 2.0 35.0 112-113 22.399753382275765 30.5 2.0 34.0 2.0 35.0 114-115 21.825885257016374 30.0 2.0 34.0 2.0 35.0 116-117 21.09894492890161 29.0 2.0 34.0 2.0 35.0 118-119 20.289429966626003 29.0 2.0 34.0 2.0 35.0 120-121 18.696233734606402 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6921049336462772 1101 2 0.6477530549572919 1101 3 0.6022801574453638 1101 4 0.8964755457525655 1101 5 0.9349974245144566 1101 6 0.9568453916339408 1101 7 0.8038533534762067 1101 8 0.8572143695955887 1101 9 0.9639156301772189 1101 10-11 0.9561556121700789 1101 12-13 1.0558721288185495 1101 14-15 1.2839297100475093 1101 16-17 1.1087777463431934 1101 18-19 0.9620449261693551 1101 20-21 1.1612720499001696 1101 22-23 0.9668053370146126 1101 24-25 0.944522793269229 1101 26-27 1.1269327075327595 1101 28-29 1.2664808237082923 1101 30-31 1.2956006873397783 1101 32-33 1.208430175866468 1101 34-35 1.2211101351779377 1101 36-37 1.009345520994394 1101 38-39 1.3639487814849645 1101 40-41 1.3986124956436328 1101 42-43 1.309869390880138 1101 44-45 1.359592665225037 1101 46-47 1.3709410773689967 1101 48-49 1.5428347107850584 1101 50-51 1.225152833576523 1101 52-53 1.3281828426807962 1101 54-55 1.5403947655411443 1101 56-57 1.5630928700884965 1101 58-59 1.4951052978470045 1101 60-61 1.440249718000235 1101 62-63 1.386419124002753 1101 64-65 1.5788409010606692 1101 66-67 1.485084524383808 1101 68-69 1.4066077694884846 1101 70-71 1.3016238617947202 1101 72-73 1.1755687478094146 1101 74-75 1.0574874178323306 1101 76-77 1.0784768666659836 1101 78-79 1.4238996444178689 1101 80-81 1.470405222772758 1101 82-83 1.5742232390453204 1101 84-85 1.6273473291641594 1101 86-87 1.4802512148669962 1101 88-89 1.6299917065882354 1101 90-91 1.663353326883545 1101 92-93 1.656101760547422 1101 94-95 1.517590818361338 1101 96-97 1.601289384047238 1101 98-99 1.7135436070650307 1101 100-101 1.7254399838896255 1101 102-103 1.9147152549595532 1101 104-105 1.9781885057917954 1101 106-107 1.7674557979096015 1101 108-109 1.9580557592353394 1101 110-111 2.296664463728625 1101 112-113 2.1611035829548157 1101 114-115 2.1560569403661702 1101 116-117 2.342968790726612 1101 118-119 2.2422191570578285 1101 120-121 1.9436591557505274 1102 1 0.7787080146580792 1102 2 0.7449156631067595 1102 3 0.8776963264036048 1102 4 1.0161760856376176 1102 5 1.075799954386035 1102 6 1.096402228423095 1102 7 0.932284273967511 1102 8 0.9957001499600366 1102 9 0.9703187322470797 1102 10-11 0.9650076212177794 1102 12-13 1.0948945266052732 1102 14-15 1.3118350867507615 1102 16-17 1.0287972432008274 1102 18-19 1.0991993289373951 1102 20-21 1.1013159450600796 1102 22-23 1.0130869558710174 1102 24-25 1.1608703819399935 1102 26-27 1.306403460430964 1102 28-29 1.161478264064968 1102 30-31 1.2515023897748918 1102 32-33 1.1562844323173138 1102 34-35 1.1622524985456266 1102 36-37 1.1683979446680084 1102 38-39 1.3543439468451837 1102 40-41 1.3339871293967889 1102 42-43 1.3697104025383098 1102 44-45 1.5086112870840225 1102 46-47 1.562820109714906 1102 48-49 1.6141492782416478 1102 50-51 1.4057256853822508 1102 52-53 1.4081564474936528 1102 54-55 1.4039407247538662 1102 56-57 1.5688722179422072 1102 58-59 1.4232860631259001 1102 60-61 1.6891977227491886 1102 62-63 1.6437620327015487 1102 64-65 1.5082573749976866 1102 66-67 1.4672203924296667 1102 68-69 1.3980267921767293 1102 70-71 1.2313360562259525 1102 72-73 1.345806449721703 1102 74-75 1.5290396145872087 1102 76-77 1.4694583900349798 1102 78-79 1.6442504426272109 1102 80-81 1.5869515922545467 1102 82-83 1.5799176285250027 1102 84-85 1.4218032506539053 1102 86-87 1.56168399790727 1102 88-89 1.5843710566203661 1102 90-91 1.8886418767457585 1102 92-93 1.913595524820611 1102 94-95 1.89638008891745 1102 96-97 1.927924560444783 1102 98-99 2.1077953016941358 1102 100-101 2.352370154641438 1102 102-103 2.122797011056406 1102 104-105 2.1385252895001194 1102 106-107 2.182691303248543 1102 108-109 2.2252493009466363 1102 110-111 2.114757148231707 1102 112-113 2.0973027486200166 1102 114-115 2.0843542511068662 1102 116-117 2.038302433879654 1102 118-119 2.0258628547076754 1102 120-121 1.7786603720349419 1103 1 0.553224693616837 1103 2 0.7469321995693683 1103 3 0.6156148116954121 1103 4 0.8294767628829689 1103 5 0.8910420337589855 1103 6 0.8224373982007833 1103 7 0.7011926498050016 1103 8 0.6927602384285549 1103 9 0.6783881598270796 1103 10-11 0.7477743956057878 1103 12-13 0.8010998454252984 1103 14-15 0.9389213599668409 1103 16-17 0.9042517195663322 1103 18-19 0.9106013529009331 1103 20-21 0.9963537108585854 1103 22-23 0.962008994066899 1103 24-25 1.0749227479806507 1103 26-27 1.0507757260161625 1103 28-29 1.0098856752466858 1103 30-31 1.0706877263162582 1103 32-33 0.9160726225816305 1103 34-35 0.9816368978941199 1103 36-37 0.8981380144133979 1103 38-39 0.9928527980152992 1103 40-41 1.0865827184068024 1103 42-43 1.178913235879996 1103 44-45 1.222865047687037 1103 46-47 1.2450968700605571 1103 48-49 1.298484460282637 1103 50-51 1.4238352191521102 1103 52-53 1.2055795988867501 1103 54-55 1.1775441329163883 1103 56-57 1.149411475200445 1103 58-59 1.2963988527169477 1103 60-61 1.1811566216771077 1103 62-63 1.2450819788811778 1103 64-65 1.2681612894101768 1103 66-67 1.2951895372627504 1103 68-69 1.2144865659446538 1103 70-71 1.2873353169731843 1103 72-73 1.0532372354541266 1103 74-75 1.088082679515363 1103 76-77 1.3741871896301205 1103 78-79 1.367359691404765 1103 80-81 1.442121093587101 1103 82-83 1.4433152031541248 1103 84-85 1.6540378666829092 1103 86-87 1.5888079246521585 1103 88-89 1.729201987094335 1103 90-91 1.7608228278600784 1103 92-93 1.719873732581732 1103 94-95 1.879235642726588 1103 96-97 1.7198477921538924 1103 98-99 1.6815189247926767 1103 100-101 1.5646160149122998 1103 102-103 1.94248281135798 1103 104-105 1.9072099895586732 1103 106-107 1.8778721697287857 1103 108-109 2.0639630874925814 1103 110-111 2.011840381529865 1103 112-113 1.9985572329307573 1103 114-115 1.903870351444887 1103 116-117 2.00123961639391 1103 118-119 2.03769737608469 1103 120-121 2.1408640016278504 1104 1 0.4525889837145556 1104 2 0.5804382475803003 1104 3 0.6656612311065935 1104 4 0.8527673933552293 1104 5 1.0111649047040316 1104 6 0.7479156979690202 1104 7 0.6192674468746233 1104 8 0.7469540580155254 1104 9 0.731028671272334 1104 10-11 0.8434956048932634 1104 12-13 0.9048101388454342 1104 14-15 1.0574083119536581 1104 16-17 0.8893873923780404 1104 18-19 0.7837544110746819 1104 20-21 0.8856467870606011 1104 22-23 0.9108740226041547 1104 24-25 0.9947025873447615 1104 26-27 0.904493887133107 1104 28-29 1.0061243541929556 1104 30-31 1.0431114489956812 1104 32-33 0.9018798595139472 1104 34-35 0.9526893934151488 1104 36-37 0.7671650275884261 1104 38-39 1.0545231774855495 1104 40-41 1.002338792830713 1104 42-43 0.8446176082963035 1104 44-45 1.0337469715412198 1104 46-47 1.1270847897740808 1104 48-49 1.0763276773164279 1104 50-51 0.6364170151000295 1104 52-53 0.8831580117250368 1104 54-55 0.9689456394679397 1104 56-57 0.8164865127967929 1104 58-59 0.9561192797305722 1104 60-61 0.6621122995690989 1104 62-63 0.8974208203515879 1104 64-65 0.8559305051356851 1104 66-67 0.7532153747790744 1104 68-69 0.70249282413549 1104 70-71 0.8450617324280394 1104 72-73 0.6285912256127588 1104 74-75 0.8172265392275868 1104 76-77 1.0511491122254704 1104 78-79 0.9056804326766787 1104 80-81 0.9018873780335674 1104 82-83 0.9592866276793117 1104 84-85 1.0494182923028283 1104 86-87 1.0579480078699106 1104 88-89 1.183853144657327 1104 90-91 1.3350129608691148 1104 92-93 1.1764378644263473 1104 94-95 1.252683739530731 1104 96-97 1.3583344294328406 1104 98-99 1.3587298037942404 1104 100-101 1.434068445693427 1104 102-103 1.4666180952806762 1104 104-105 1.5196916409657568 1104 106-107 1.5008105521752775 1104 108-109 1.6065306991071004 1104 110-111 1.322326110651808 1104 112-113 1.4923917685230919 1104 114-115 1.7167321250035812 1104 116-117 1.7120118420754231 1104 118-119 1.5409551645425559 1104 120-121 1.626481851858376 1105 1 0.6748334683194557 1105 2 0.9361065918607743 1105 3 0.8716129578849987 1105 4 1.1865576565825577 1105 5 1.2034523777494641 1105 6 0.9763079064877545 1105 7 1.0148235887463954 1105 8 1.0576739904084178 1105 9 1.1137755671577807 1105 10-11 0.9908569835386984 1105 12-13 1.1096281372544468 1105 14-15 1.1747958169378947 1105 16-17 1.0029764609750558 1105 18-19 0.9217984866248372 1105 20-21 1.1515814731973748 1105 22-23 0.8551785005832429 1105 24-25 1.038151264066535 1105 26-27 1.0772158667835257 1105 28-29 1.12000702173777 1105 30-31 1.0286412859887477 1105 32-33 0.9524706282075712 1105 34-35 0.9321667277747565 1105 36-37 0.9111286927348559 1105 38-39 1.0164986421356375 1105 40-41 1.0172617528635683 1105 42-43 1.3063376616995725 1105 44-45 1.2862752949126843 1105 46-47 1.4400419263114799 1105 48-49 1.0860843869450818 1105 50-51 1.0519582191334393 1105 52-53 1.1823199652689453 1105 54-55 1.3234073515862335 1105 56-57 1.3086927501874186 1105 58-59 1.2593430160152366 1105 60-61 1.4038761547613383 1105 62-63 1.4589585646784862 1105 64-65 1.6480837756890985 1105 66-67 1.6999797358343613 1105 68-69 1.5042475737927923 1105 70-71 1.2816949460073879 1105 72-73 1.2099389638097833 1105 74-75 1.416716411055777 1105 76-77 1.190779217226769 1105 78-79 1.4332455055110387 1105 80-81 1.5995112103330236 1105 82-83 1.8248703837223914 1105 84-85 1.6383946437010941 1105 86-87 1.6170055354096888 1105 88-89 1.6798434360542807 1105 90-91 1.7985025430659078 1105 92-93 1.9138855175193505 1105 94-95 1.9565579561869164 1105 96-97 2.0635908424868816 1105 98-99 1.9940065108836649 1105 100-101 2.240854052996898 1105 102-103 2.545985528412526 1105 104-105 2.3596330997160955 1105 106-107 2.1059358375911508 1105 108-109 2.5652651686154577 1105 110-111 2.3146756759071465 1105 112-113 2.46434662173354 1105 114-115 2.3480913691053154 1105 116-117 2.1969078624210354 1105 118-119 2.0919888113787266 1105 120-121 2.0541240936053704 1106 1 0.4541778158431562 1106 2 0.6311247279182197 1106 3 0.5020935849260582 1106 4 1.037819605980637 1106 5 0.8079813136698775 1106 6 0.898971006195616 1106 7 0.9680502920466907 1106 8 0.9404257132047604 1106 9 0.7407839181795808 1106 10-11 1.0176556552252762 1106 12-13 0.9059818170579206 1106 14-15 1.0489176340899462 1106 16-17 1.0573579499737775 1106 18-19 1.090073039162597 1106 20-21 1.040771625498941 1106 22-23 0.7559070415903548 1106 24-25 1.0198275625548803 1106 26-27 0.7724629630345987 1106 28-29 0.6936908879630437 1106 30-31 0.8352508331123758 1106 32-33 0.7512172002603137 1106 34-35 0.8982547965385663 1106 36-37 1.035274180583606 1106 38-39 1.117886691790737 1106 40-41 0.9077611098813634 1106 42-43 0.6999142092897159 1106 44-45 0.8585371978301168 1106 46-47 0.43814654906943673 1106 48-49 0.8725850316487893 1106 50-51 0.885490386319141 1106 52-53 0.5849972501797822 1106 54-55 0.659637978196919 1106 56-57 0.5185537042111719 1106 58-59 0.7618047684731124 1106 60-61 0.7924637802375507 1106 62-63 0.7883840811730813 1106 64-65 0.570904393124156 1106 66-67 0.8613209261175001 1106 68-69 0.7870685010987906 1106 70-71 0.5544046124313233 1106 72-73 0.2528130511968314 1106 74-75 0.4917843674741498 1106 76-77 0.5586829575411514 1106 78-79 0.4456343177929476 1106 80-81 0.38175457263928436 1106 82-83 0.5660911375006634 1106 84-85 0.4767685443048286 1106 86-87 0.3817876703171663 1106 88-89 0.5394056228479265 1106 90-91 0.5081147395860555 1106 92-93 0.5807346637388378 1106 94-95 0.40725921133637044 1106 96-97 0.6647921088261164 1106 98-99 0.7647493575991184 1106 100-101 0.5104469342285327 1106 102-103 0.7921719639843339 1106 104-105 0.9426897010860706 1106 106-107 0.905440732691229 1106 108-109 0.709230346007967 1106 110-111 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-1.1579481388225972 2316 84-85 -1.6849186952580304 2316 86-87 -1.5160685552770872 2316 88-89 -1.3614732023567093 2316 90-91 -1.020700418838345 2316 92-93 -1.2684978929785835 2316 94-95 -1.4445547852217082 2316 96-97 -1.6839643447734822 2316 98-99 -1.0716720941222775 2316 100-101 -1.4439519965050174 2316 102-103 -1.4391530004171322 2316 104-105 -1.733616961736832 2316 106-107 -1.7515073152859877 2316 108-109 -1.4828619743177072 2316 110-111 -1.58476960058033 2316 112-113 -1.3220924283689683 2316 114-115 -1.6365023193625703 2316 116-117 -1.4442919520798263 2316 118-119 -1.3822959906161465 2316 120-121 -1.7757222614126107 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13055.0 3 1952.0 4 1673.0 5 1911.0 6 2322.0 7 2559.0 8 3342.0 9 4106.0 10 5590.0 11 7014.0 12 7303.0 13 8152.0 14 9837.0 15 10078.0 16 7909.0 17 7948.0 18 8231.0 19 8731.0 20 9577.0 21 10654.0 22 11772.0 23 12813.0 24 14282.0 25 16202.0 26 18514.0 27 21104.0 28 24112.0 29 28543.0 30 32793.0 31 38670.0 32 46122.0 33 55334.0 34 68466.0 35 86279.0 36 110607.0 37 116566.0 38 40319.0 39 191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.81149426873312 18.04765534900995 12.769761631269525 21.371088750987404 2 14.305410891992324 22.324404095178487 38.22243572126366 25.14774929156553 3 22.595884359782893 25.20247187538205 30.375048729346254 21.826595035488804 4 11.252992817238628 15.916136532437756 42.12225917912617 30.70861147119745 5 10.934690360821829 38.73168526158804 34.600625379905026 15.732998997685103 6 25.97143618352535 37.11935191069396 19.320424293660853 17.58878761211984 7 25.222167245080023 32.68548730316357 24.100864217607988 17.991481234148416 8 22.61413875313872 30.25399282347147 21.527509449326583 25.604358974063228 9 22.294096409584437 19.910444352422928 26.265965508848872 31.529493729143766 10-11 23.93124287336639 24.179308841264888 28.96754148227058 22.921906803098143 12-13 28.107513844723375 22.66341272647464 27.826379872330335 21.402693556471647 14-15 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27.64237916113445 31.019872120002468 19.824977164286157 40-41 20.21166713983349 28.954232073324697 30.174771668155408 20.659329118686394 42-43 19.119940683124984 29.020434894248055 27.823249560749307 24.036374861877658 44-45 20.495123148928755 27.22940732828885 27.763803799575644 24.51166572320675 46-47 22.347168822745665 28.506809262743886 28.0859223303772 21.060099584133244 48-49 21.858160628489056 27.41831985125667 29.048979813239868 21.67453970701441 50-51 19.05427131963807 28.112215551433472 31.619557910736685 21.21395521819177 52-53 20.194086227842114 29.43895056081859 28.43296868822988 21.93399452310942 54-55 19.324811525851388 27.900522354595147 29.244989556947964 23.529676562605506 56-57 21.4536743795243 26.37333339488846 28.535534236962622 23.637457988624615 58-59 23.814264184852227 25.818429508160794 28.32558270981331 22.04172359717367 60-61 21.78113791941507 26.66375478325894 31.164691817338365 20.390415479987627 62-63 20.453338505372916 27.575456363224955 30.39975940780147 21.571445723600664 64-65 19.516017406472532 30.241870081773943 29.151108337917375 21.091004173836147 66-67 19.992507391590962 27.575916055078174 27.65430559451943 24.777270958811435 68-69 19.541025553684328 28.333984766283756 28.484555265666287 23.640434414365625 70-71 22.278297569409133 27.268451318842857 27.457864519290332 22.995386592457677 72-73 20.89790446583092 25.757532857386373 31.202339093427945 22.142223583354763 74-75 19.43306853648262 29.36477071196972 29.332583650243944 21.86957710130372 76-77 20.441355256832217 27.23994351170445 28.515206075958726 23.803495155504603 78-79 21.140784161486202 27.129892356758806 28.983033542397685 22.7462899393573 80-81 22.410220914806484 26.60783295841264 28.57132145123147 22.410624675549403 82-83 21.067679491848324 26.158430344125478 30.622648554779186 22.151241609247013 84-85 21.03789915869407 27.36413890203229 29.291756586767608 22.30620535250603 86-87 21.194862096447938 28.44285649154619 28.184772465343045 22.177508946662826 88-89 20.146741062565503 27.237128847725415 27.245093241115658 25.37103684859342 90-91 20.72576660771756 25.251591856712118 28.744845801146045 25.277795734424274 92-93 22.21426382342128 26.79501171923923 28.382664183338424 22.608060274001065 94-95 21.002884033237084 26.496398854235988 29.412868086501376 23.08784902602556 96-97 18.456041365227975 27.477818825703554 31.339331422209625 22.726808386858842 98-99 20.37636841303334 28.897466560164002 28.028342483020367 22.69782254378229 100-101 19.432540362772922 27.428504806940325 28.36322772897826 24.775727101308497 102-103 21.53306935332979 25.751382085949903 28.300861022665693 24.414687538054615 104-105 23.176414980548003 25.71455284069562 28.952800989423032 22.156231189333344 106-107 22.236842940588133 26.291610612563854 30.051578431593217 21.419968015254796 108-109 21.595236418749128 26.13268953958281 29.081301085161076 23.190772956506983 110-111 20.70776152928941 27.678951724328986 27.961693890483723 23.651592855897878 112-113 22.391848254248586 25.86274662580405 26.56381753802734 25.181587581920024 114-115 21.501518763934506 25.60099823450546 27.569937304455177 25.327545697104853 116-117 23.102122229475043 26.20486777836839 26.514565185648188 24.178444806508377 118-119 23.04422858264217 25.74094275044875 28.144915992819996 23.06991267408908 120-121 22.099978125348105 28.608408523841927 26.622663409132752 22.668949941677212 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2255.0 1 2277.5 2 1600.0 3 858.5 4 752.5 5 656.5 6 651.0 7 726.5 8 830.5 9 882.5 10 827.0 11 766.5 12 811.5 13 891.0 14 955.5 15 1036.0 16 1100.5 17 1175.0 18 1284.0 19 1435.5 20 1648.0 21 1902.5 22 2226.5 23 2642.5 24 3145.5 25 3864.5 26 4833.5 27 5992.5 28 7732.0 29 9739.5 30 11382.5 31 13045.5 32 14938.5 33 16445.0 34 17831.5 35 19544.5 36 21102.0 37 22706.5 38 24031.5 39 24614.0 40 25290.5 41 25971.5 42 26556.0 43 27495.0 44 27965.0 45 28977.5 46 33288.0 47 47406.5 48 78315.5 49 78410.5 50 47998.5 51 32260.0 52 24412.5 53 20317.5 54 17891.5 55 15969.0 56 13879.0 57 11665.5 58 9685.0 59 8029.5 60 6477.5 61 5031.0 62 3738.5 63 2628.0 64 1779.5 65 1195.5 66 755.5 67 444.0 68 259.5 69 160.0 70 93.5 71 61.0 72 45.0 73 32.0 74 19.5 75 15.0 76 19.5 77 15.5 78 9.0 79 7.0 80 6.5 81 4.5 82 5.0 83 6.0 84 5.0 85 2.5 86 1.0 87 1.0 88 3.0 89 4.5 90 2.0 91 0.5 92 0.5 93 0.5 94 1.0 95 1.5 96 2.0 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5636649886295166 2 0.987728567296226 3 0.8688215514392894 4 0.8640195373373747 5 0.873509231872111 6 0.8680212157556368 7 0.8696218871229419 8 0.873509231872111 9 0.8787685806503985 10-11 0.8833990942486735 12-13 0.890259114394266 14-15 0.8910594500779184 16-17 0.896890467201672 18-19 0.9019211486417732 20-21 0.9047794903691033 22-23 0.896890467201672 24-25 0.9028929848290654 26-27 0.9051796582109296 28-29 0.9107820079964968 30-31 0.9206718703730594 32-33 0.9258168854822536 34-35 0.9289610613823169 36-37 0.9329055729660326 38-39 0.9281035588641179 40-41 0.9296470633968762 42-43 0.9261598864895334 44-45 0.9308475669223548 46-47 0.9294755628932363 48-49 0.9345634111678842 50-51 1.0101379664384946 52-53 0.9450249418899126 54-55 0.9452536092280991 56-57 0.9376504202334008 58-59 0.8819127565504616 60-61 0.9225583759130972 62-63 0.9646331661393979 64-65 0.9608029882247755 66-67 0.9656621691612367 68-69 0.982526385352485 70-71 1.0666187989705396 72-73 1.0679336361651115 74-75 1.0722783155906535 76-77 1.065704129617794 78-79 0.89574713051074 80-81 0.8898589465524397 82-83 0.9463969459190311 84-85 0.9287323940441304 86-87 0.9430812695153281 88-89 0.9463397790844846 90-91 0.9545718032591956 92-93 0.9515419610282256 94-95 0.9502842906682003 96-97 0.9491409539772683 98-99 0.9490837871427216 100-101 0.9527996313882509 102-103 0.9466256132572176 104-105 0.9454822765662856 106-107 0.9469686142644972 108-109 0.9501127901645605 110-111 0.9485121187972555 112-113 0.9507416253445731 114-115 0.9493696213154545 116-117 0.9493696213154545 118-119 0.9533141328991702 120-121 0.9527996313882509 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 874633.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.50960101713802 #Duplication Level Percentage of deduplicated Percentage of total 1 88.09996489987503 56.83293585314802 2 7.856813059777138 10.13679751504925 3 1.6789058440802558 3.249166384408759 4 0.7169065999666226 1.8498943492159918 5 0.4009303886347154 1.2931929703235787 6 0.27448597525655455 1.0624188449160212 7 0.18137253023473232 0.8190188692637969 8 0.12577763487182553 0.6491092033968584 9 0.10351035798154697 0.600967070507765 >10 0.508565101279823 5.762591543675803 >50 0.027539321581645956 1.206668248218623 >100 0.016345087037195765 2.106652484400449 >500 0.0033756157806798756 1.5081573338469414 >1k 0.00479692768833456 6.76304096914463 >5k 1.7766398845683556E-4 0.8561391658816515 >10k+ 5.329919653705066E-4 5.303249194601845 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18075 2.0665810688597386 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17455 1.99569419402195 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10742 1.2281722733992428 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7470 0.8540725081262656 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4771 0.5454859352436965 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4392 0.5021534746573706 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3417 0.3906781472914926 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3342 0.3821031221095019 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3092 0.3535197048361999 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2808 0.32104894281372875 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2708 0.3096155759044079 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2562 0.2929228602167995 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2480 0.2835474993511564 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2307 0.2637677745980314 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2144 0.24513138653583844 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2110 0.2412440417866694 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2045 0.23381235329561087 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1921 0.219634978328053 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1830 0.2092306144405711 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1723 0.1969969118475978 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1693 0.19356690177480154 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1601 0.18304820421822637 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1579 0.1805328634981758 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1579 0.1805328634981758 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1574 0.17996119515270975 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1366 0.15617979198132245 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1358 0.15526512262857678 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1276 0.1458897617629337 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1147 0.13114071844990985 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1125 0.12862537772985927 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1059 0.12107935556970753 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 930 0.10633031225668367 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 914 0.10450097355119232 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.716683454660412E-5 0.0 16-17 0.0 0.0 0.0 1.1433366909320824E-4 0.0 18-19 0.0 0.0 0.0 5.716683454660412E-4 0.0 20-21 0.0 0.0 0.0 0.001429170863665103 0.0 22-23 0.0 0.0 0.0 0.0031441759000632266 0.0 24-25 0.0 0.0 0.0 0.0062311849655798494 0.0 26-27 0.0 0.0 0.0 0.009947029211109116 0.0 28-29 0.0 0.0 0.0 0.020065558925858047 0.0 30-31 0.0 0.0 0.0 0.044189963104524985 0.0 32-33 0.0 0.0 0.0 0.08529291714353335 0.0 34-35 0.0 0.0 0.0 0.12107935556970753 0.0 36-37 0.0 5.716683454660412E-5 0.0 0.15452195377947092 0.0 38-39 0.0 1.1433366909320824E-4 0.0 0.19345256810570832 0.0 40-41 0.0 1.1433366909320824E-4 0.0 0.2318686809210263 0.0 42-43 0.0 1.1433366909320824E-4 0.0 0.34740285353971323 0.0 44-45 0.0 1.1433366909320824E-4 0.0 0.45944984925105725 0.0 46-47 0.0 1.1433366909320824E-4 0.0 0.5453144347400567 0.0 48-49 0.0 1.1433366909320824E-4 0.0 0.6447275600166013 0.0 50-51 0.0 1.1433366909320824E-4 0.0 0.7640919105499107 0.0 52-53 0.0 1.1433366909320824E-4 0.0 1.026830682126103 0.0 54-55 0.0 1.1433366909320824E-4 0.0 1.1863833173456753 0.0 56-57 0.0 1.1433366909320824E-4 0.0 1.606788218601402 0.0 58-59 0.0 1.1433366909320824E-4 0.0 2.077385600589047 0.0 60-61 0.0 1.1433366909320824E-4 0.0 2.4113542480103085 0.0 62-63 0.0 1.1433366909320824E-4 0.0 2.954382009368501 0.0 64-65 0.0 1.1433366909320824E-4 0.0 3.2038580753298813 0.0 66-67 0.0 1.1433366909320824E-4 0.0 3.9381088982464645 0.0 68-69 0.0 1.1433366909320824E-4 0.0 4.596327831216064 0.0 70-71 0.0 1.1433366909320824E-4 0.0 4.891766032152914 0.0 72-73 0.0 1.1433366909320824E-4 0.0 5.518943373963708 0.0 74-75 0.0 1.1433366909320824E-4 0.0 6.01703800336827 0.0 76-77 0.0 1.1433366909320824E-4 0.0 6.217407758454117 0.0 78-79 0.0 1.1433366909320824E-4 0.0 6.46768416009915 0.0 80-81 0.0 1.1433366909320824E-4 0.0 6.977326490082126 0.0 82-83 0.0 1.1433366909320824E-4 0.0 7.335133707509321 0.0 84-85 0.0 1.7150050363981236E-4 0.0 8.153305443540319 0.0 86-87 0.0 2.2866733818641648E-4 0.0 9.583390976558167 0.0 88-89 0.0 2.2866733818641648E-4 0.0 11.420904539389664 0.0 90-91 0.0 2.2866733818641648E-4 0.0 12.711674496617437 0.0 92-93 0.0 2.2866733818641648E-4 0.0 13.617711657346568 0.0 94-95 0.0 2.2866733818641648E-4 0.0 14.374829214081792 0.0 96-97 0.0 2.2866733818641648E-4 0.0 15.408119748511663 0.0 98-99 0.0 2.2866733818641648E-4 0.0 16.33205012845388 0.0 100-101 0.0 2.2866733818641648E-4 0.0 16.84512246850965 0.0 102-103 0.0 2.2866733818641648E-4 0.0 17.497053049679124 0.0 104-105 0.0 2.2866733818641648E-4 0.0 18.38393932083514 0.0 106-107 0.0 2.2866733818641648E-4 0.0 19.297579670558967 0.0 108-109 0.0 2.2866733818641648E-4 0.0 20.333900047219807 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 195 0.0 70.832115 2 TACCTGG 1165 0.0 59.280098 2 TCGTGCG 30 0.004868737 57.44479 9 TAACGCA 280 0.0 53.332355 4 ACCTGGG 1390 0.0 49.170834 3 GTATAAC 330 0.0 47.16363 1 ATAACGC 295 0.0 46.72665 3 TACACGG 110 1.2918463E-8 41.85534 2 GTACCCG 130 1.2496457E-9 39.907684 1 GTACCTG 1820 0.0 38.324047 1 CCTGGGG 945 0.0 37.07434 4 TACGCAG 420 0.0 36.922398 4 AGTACGC 410 0.0 36.495815 2 GAGTACG 460 0.0 35.087917 1 GTACGCA 445 0.0 33.557438 3 GTACACG 165 1.2822056E-8 31.442421 1 TACCCGG 165 1.3025783E-8 31.391504 2 GTACCGG 75 0.004565683 30.743698 1 ACGCAGT 505 0.0 29.57383 5 CTGGGGG 620 0.0 25.941309 5 >>END_MODULE