##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003861_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1006639 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.621624038011642 31.0 31.0 34.0 30.0 34.0 2 31.833515291976568 31.0 31.0 34.0 30.0 34.0 3 31.871926281417668 31.0 31.0 34.0 30.0 34.0 4 35.609264095668856 37.0 35.0 37.0 33.0 37.0 5 35.492047297988655 37.0 35.0 37.0 33.0 37.0 6 35.5300748331825 37.0 35.0 37.0 33.0 37.0 7 35.4631650472513 37.0 35.0 37.0 33.0 37.0 8 35.46269814700205 37.0 35.0 37.0 33.0 37.0 9 37.01316559362393 39.0 37.0 39.0 33.0 39.0 10-11 36.93281802115754 39.0 37.0 39.0 33.0 39.0 12-13 36.890868523870026 39.0 37.0 39.0 33.0 39.0 14-15 37.948127382308854 40.0 37.0 41.0 33.0 41.0 16-17 37.98621948881377 40.0 37.0 41.0 33.0 41.0 18-19 38.016235711113914 40.0 37.0 41.0 33.0 41.0 20-21 37.94819344372709 40.0 37.0 41.0 32.5 41.0 22-23 37.85712156989745 40.0 37.0 41.0 32.5 41.0 24-25 37.75401211357796 39.5 37.0 41.0 32.0 41.0 26-27 37.488784956672646 39.0 36.5 41.0 32.0 41.0 28-29 37.31721798976594 39.0 36.0 41.0 31.5 41.0 30-31 37.08574027034518 39.0 36.0 40.0 31.0 41.0 32-33 36.82142009200915 39.0 35.0 40.0 30.5 41.0 34-35 36.75848491862524 39.0 35.0 40.0 30.0 41.0 36-37 36.52877247950855 38.5 35.0 40.0 30.0 41.0 38-39 36.286641487166705 38.0 35.0 40.0 30.0 41.0 40-41 36.434656316713344 39.0 35.0 40.0 30.0 41.0 42-43 36.28853143977136 39.0 35.0 40.5 30.0 41.0 44-45 36.122602045023086 39.0 35.0 41.0 30.0 41.0 46-47 35.80918035164542 39.0 35.0 40.0 28.5 41.0 48-49 35.530987772180495 39.0 35.0 40.0 27.0 41.0 50-51 35.23659325736436 38.0 34.0 40.0 27.0 41.0 52-53 35.06422858641479 38.0 34.0 40.0 26.0 41.0 54-55 34.85246697177439 38.0 34.0 40.0 26.0 41.0 56-57 34.58419204898678 38.0 34.0 40.0 24.5 41.0 58-59 34.321268597779344 38.0 33.5 40.0 24.0 41.0 60-61 33.85016773639805 37.0 33.0 40.0 22.5 41.0 62-63 33.658596080620754 37.0 33.0 40.0 22.5 41.0 64-65 33.199846220939186 36.5 32.0 39.5 21.5 41.0 66-67 32.83962920172972 36.0 32.0 39.0 20.5 41.0 68-69 32.56243648418152 36.0 31.5 39.0 20.0 40.0 70-71 32.1234653137818 35.0 31.0 38.5 18.5 40.0 72-73 31.73211945891228 35.0 31.0 38.0 18.0 40.0 74-75 31.358660353910388 35.0 31.0 37.0 16.0 39.5 76-77 30.392156473174595 34.0 29.5 36.0 13.0 39.0 78-79 30.756734042690578 34.0 30.0 36.0 12.5 39.0 80-81 30.539860863725725 34.0 30.0 36.0 9.5 38.5 82-83 30.211721878448976 34.0 30.0 35.5 7.5 37.0 84-85 29.86407093307531 34.0 30.0 35.0 6.0 37.0 86-87 29.53696856569237 34.0 29.5 35.0 2.0 36.5 88-89 29.209037698718213 34.0 29.0 35.0 2.0 36.0 90-91 28.909983618755085 34.0 29.0 35.0 2.0 36.0 92-93 28.621566917236468 34.0 29.0 35.0 2.0 35.5 94-95 28.26220621295221 34.0 29.0 35.0 2.0 35.0 96-97 27.826458641081857 34.0 29.0 35.0 2.0 35.0 98-99 27.465412128876387 34.0 27.0 35.0 2.0 35.0 100-101 26.493458429486637 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09798175470310255 1101 2 0.12076537917950603 1101 3 -0.004714109004609668 1101 4 0.1020831771722186 1101 5 0.05693938227391726 1101 6 0.1802196113336123 1101 7 0.14567091063452864 1101 8 0.13385263806704728 1101 9 0.1301399104368386 1101 10-11 0.21125525195947858 1101 12-13 0.18937352086590664 1101 14-15 0.12685996751199724 1101 16-17 0.2122447475660607 1101 18-19 0.11106108356349864 1101 20-21 0.1398774479866134 1101 22-23 0.044933317064284495 1101 24-25 0.06409111769513487 1101 26-27 0.13407918186422307 1101 28-29 0.04042454452497424 1101 30-31 0.28210123301252565 1101 32-33 0.10081991839329163 1101 34-35 0.21520344741056618 1101 36-37 0.03167735015087203 1101 38-39 0.17250986498178378 1101 40-41 0.34670580605286716 1101 42-43 0.20446001032549788 1101 44-45 0.24560274674406912 1101 46-47 0.34350442287682625 1101 48-49 0.45948790993567457 1101 50-51 0.2951367735782924 1101 52-53 0.34559114532798674 1101 54-55 0.26318586342944883 1101 56-57 0.5231546691471252 1101 58-59 0.2921402381911946 1101 60-61 0.2142936029601188 1101 62-63 0.25930843415378746 1101 64-65 0.17537138451205436 1101 66-67 0.11780744764016049 1101 68-69 -0.010441929429930497 1101 70-71 0.06202521921266069 1101 72-73 -0.05688384815691805 1101 74-75 -0.1874120267112751 1101 76-77 -0.13378040736060015 1101 78-79 0.008516888855773175 1101 80-81 -0.26921031189711897 1101 82-83 -0.053479837432131916 1101 84-85 -0.37367962988418313 1101 86-87 -0.19484744862685233 1101 88-89 -0.21289788376981988 1101 90-91 -0.2762293398145097 1101 92-93 0.0567285486536413 1101 94-95 0.049390088768014095 1101 96-97 0.07421841788306693 1101 98-99 -0.2682509820176655 1101 100-101 -0.038355075919156434 1102 1 0.06779238430097934 1102 2 0.19651241636927708 1102 3 0.18949701994772283 1102 4 0.1422281742450835 1102 5 0.1278956955008539 1102 6 0.04885590397510242 1102 7 -0.11045387853878452 1102 8 -0.03376651763738181 1102 9 0.03542212323780092 1102 10-11 0.08131241253834531 1102 12-13 0.18921592756561267 1102 14-15 0.1904200703942891 1102 16-17 0.19096656196217054 1102 18-19 0.021693883870028685 1102 20-21 -0.03999606161899294 1102 22-23 -0.0037509333815322066 1102 24-25 -0.030537619457220444 1102 26-27 -0.23408826368603286 1102 28-29 0.10735731258246517 1102 30-31 0.030248598760451273 1102 32-33 -0.08319146877608574 1102 34-35 -0.35664224262336575 1102 36-37 -0.4515775891771199 1102 38-39 -0.3188298072915643 1102 40-41 -0.11480715704951905 1102 42-43 0.07121754362235322 1102 44-45 0.11324544312330431 1102 46-47 0.13496297836708493 1102 48-49 0.0457276832260618 1102 50-51 0.08101579585933649 1102 52-53 0.053004435303023456 1102 54-55 0.1217047223752914 1102 56-57 0.16898100301568775 1102 58-59 -0.03399783264207912 1102 60-61 0.01961727881121078 1102 62-63 -0.21230398408591356 1102 64-65 -0.32058568718532143 1102 66-67 -0.3201743917686173 1102 68-69 -0.21714984190581532 1102 70-71 -0.27145934001488925 1102 72-73 -0.10001440957111285 1102 74-75 -0.20378852693768934 1102 76-77 -0.002501536324171383 1102 78-79 -0.013018053034155486 1102 80-81 0.07805453136918317 1102 82-83 -0.012049935269200063 1102 84-85 -0.12639888683036915 1102 86-87 -0.23810301720437366 1102 88-89 -0.14622917578768124 1102 90-91 -0.055252351245503206 1102 92-93 -0.23182422234784283 1102 94-95 0.05781500974663345 1102 96-97 -0.04170451702668743 1102 98-99 -0.15752356954796554 1102 100-101 -0.4424478947211341 1103 1 -0.025162779765125265 1103 2 -0.04087138504867127 1103 3 0.05241045493450969 1103 4 0.07238748999676403 1103 5 0.15107556979539538 1103 6 0.06864451941362404 1103 7 0.09225727775895365 1103 8 0.09058786613689307 1103 9 0.09235973690697108 1103 10-11 0.17295721935318653 1103 12-13 0.038081580165474804 1103 14-15 0.18307768959115123 1103 16-17 0.037679749190260736 1103 18-19 0.08466969038134664 1103 20-21 0.12251445702089825 1103 22-23 0.012413532493660284 1103 24-25 -0.018607445952106616 1103 26-27 0.15267503051755682 1103 28-29 -0.06440837530573873 1103 30-31 0.015858112299575566 1103 32-33 -0.08942090961628679 1103 34-35 0.015827114511921536 1103 36-37 -0.10141360944200528 1103 38-39 -0.11549863732268051 1103 40-41 0.031869261208626654 1103 42-43 0.0738135809638365 1103 44-45 0.08998197494573645 1103 46-47 0.20053883225855174 1103 48-49 0.29028451842950886 1103 50-51 0.20084346290521182 1103 52-53 0.4462158716865403 1103 54-55 0.3424783266967566 1103 56-57 0.4587430041360321 1103 58-59 0.4086185140029457 1103 60-61 0.2871969798720784 1103 62-63 0.03344537492483113 1103 64-65 0.22501523870176499 1103 66-67 0.09084346709283153 1103 68-69 0.01593828270397779 1103 70-71 -0.036871152478337166 1103 72-73 0.3057993444913656 1103 74-75 0.22843410794149932 1103 76-77 0.33339380043270594 1103 78-79 0.21417932477071133 1103 80-81 0.27173292133248594 1103 82-83 0.31174557250040635 1103 84-85 0.3148281356480567 1103 86-87 0.24451654981015736 1103 88-89 0.20182367282040303 1103 90-91 0.16915712429079122 1103 92-93 0.054366993569910704 1103 94-95 -0.014232429627753618 1103 96-97 0.30461568641183234 1103 98-99 0.5465667315874327 1103 100-101 0.5023033348184143 1104 1 0.11629367601133112 1104 2 0.09838987022861545 1104 3 0.08615238489943522 1104 4 -0.04321843566079053 1104 5 0.17098333363459517 1104 6 0.08416565623814876 1104 7 0.21897129534067972 1104 8 0.10030713207191155 1104 9 -0.01414208215315682 1104 10-11 0.09447722901458633 1104 12-13 0.041112718046711905 1104 14-15 0.07283899770420277 1104 16-17 0.18843718771206852 1104 18-19 0.22231253566311437 1104 20-21 0.1447149159373069 1104 22-23 0.11092394114061221 1104 24-25 0.24761187785066596 1104 26-27 0.24228953066599956 1104 28-29 0.1546317496886047 1104 30-31 0.1196024072942663 1104 32-33 0.39563222807372966 1104 34-35 0.3004729135649029 1104 36-37 0.21161569176718587 1104 38-39 0.2169000060559867 1104 40-41 0.34199951396488615 1104 42-43 0.17548847491489283 1104 44-45 0.15774699665872305 1104 46-47 0.06618375574731772 1104 48-49 0.2776077072947487 1104 50-51 0.3069798345470005 1104 52-53 0.2837924206552316 1104 54-55 0.23151691693774268 1104 56-57 0.35987909901901816 1104 58-59 0.21199522298854134 1104 60-61 0.2789237786784895 1104 62-63 0.19627012621931073 1104 64-65 0.00372519044509545 1104 66-67 0.058007293207793964 1104 68-69 0.08075393618182858 1104 70-71 0.25784615098517705 1104 72-73 0.4112891069316724 1104 74-75 0.3123731656532591 1104 76-77 0.16031702250900182 1104 78-79 0.341294013618338 1104 80-81 0.2999239503217126 1104 82-83 0.5058082155972876 1104 84-85 0.43502070945459437 1104 86-87 0.37875022984679063 1104 88-89 0.36233748811661215 1104 90-91 0.4093856515817258 1104 92-93 0.5706750059888357 1104 94-95 0.7600619612261248 1104 96-97 0.649252729822706 1104 98-99 0.6103036547340288 1104 100-101 0.7830933805590945 1105 1 0.18885663349356108 1105 2 0.1258953240480487 1105 3 0.10208076736852689 1105 4 0.08233391953773861 1105 5 0.06304175042677684 1105 6 0.17263937954525943 1105 7 0.11180778058527352 1105 8 0.16803578877343028 1105 9 0.11916306454944703 1105 10-11 0.1360943488694275 1105 12-13 0.18959533775915105 1105 14-15 0.28465418736206516 1105 16-17 0.2840955657476343 1105 18-19 0.15843238169364326 1105 20-21 0.1250459326819353 1105 22-23 0.2365460288891157 1105 24-25 0.17064769542444225 1105 26-27 0.394567069151023 1105 28-29 0.4915698888424771 1105 30-31 0.4039453211459403 1105 32-33 0.46427548222024484 1105 34-35 0.4432257197495204 1105 36-37 0.3147060915769231 1105 38-39 0.48616307246779655 1105 40-41 0.5826729689117869 1105 42-43 0.6534016072423157 1105 44-45 0.6805774335221386 1105 46-47 0.7142964259314439 1105 48-49 0.5375743237539723 1105 50-51 0.5677641569080976 1105 52-53 0.5596066766309491 1105 54-55 0.6731522406582613 1105 56-57 0.6989194910480663 1105 58-59 0.41305104719792496 1105 60-61 0.7284843902109372 1105 62-63 0.623638904240785 1105 64-65 0.5922745185169234 1105 66-67 0.5152641761774888 1105 68-69 0.4817388533982836 1105 70-71 0.7256972433639213 1105 72-73 0.7548327536357498 1105 74-75 0.4863859729535953 1105 76-77 0.40993606257805837 1105 78-79 0.7357123267072119 1105 80-81 0.7757333617202704 1105 82-83 0.6059419552878786 1105 84-85 0.5632309758476204 1105 86-87 0.6865203443843413 1105 88-89 0.6748861173948875 1105 90-91 0.7968431881289213 1105 92-93 0.7585990899967392 1105 94-95 0.6789696118410937 1105 96-97 0.8305947951393513 1105 98-99 0.7863269615543125 1105 100-101 0.9092329572311364 1106 1 -0.058702081667270534 1106 2 -0.00248310524133899 1106 3 -0.01049712393401947 1106 4 -0.10300217154328806 1106 5 0.005858043742627217 1106 6 -0.01549590078808194 1106 7 -0.01131133753825253 1106 8 0.008894533320095377 1106 9 -0.042381801594302715 1106 10-11 -0.10191396958261834 1106 12-13 -0.06798093355499901 1106 14-15 0.1931323777973475 1106 16-17 0.028742069209883425 1106 18-19 0.01763466926795587 1106 20-21 -0.20495135477936088 1106 22-23 -0.22091043863199644 1106 24-25 -0.13405799412550579 1106 26-27 0.16351904968102815 1106 28-29 -0.023227574933834205 1106 30-31 0.17191965751580796 1106 32-33 0.20438130890409667 1106 34-35 0.10649041078382027 1106 36-37 0.11351200943221329 1106 38-39 0.40142920265459026 1106 40-41 0.26185027852547904 1106 42-43 0.5229558090401198 1106 44-45 0.5557356649469014 1106 46-47 0.6289889672034761 1106 48-49 0.5706955516298322 1106 50-51 0.6318827325238843 1106 52-53 0.749078447391291 1106 54-55 0.7567398744956719 1106 56-57 0.7488245521932129 1106 58-59 0.7172227056893021 1106 60-61 0.6887485236678401 1106 62-63 0.4862469348623293 1106 64-65 0.8164856218420411 1106 66-67 0.7870172296672422 1106 68-69 0.7575833194425741 1106 70-71 0.8405242813469975 1106 72-73 0.8411658910635076 1106 74-75 0.9686602859654947 1106 76-77 0.765257912830851 1106 78-79 0.8564768105336995 1106 80-81 1.0353468626282165 1106 82-83 1.0545996253678247 1106 84-85 1.0599759293953923 1106 86-87 0.980768632094275 1106 88-89 0.9960036079951884 1106 90-91 0.8608063578810992 1106 92-93 0.7049774740771504 1106 94-95 0.6951355890708371 1106 96-97 0.8519626304581145 1106 98-99 0.8600844681284521 1106 100-101 0.8221793017084806 1107 1 -0.3073784012955585 1107 2 -0.10732849577207304 1107 3 -0.18232344266184697 1107 4 -0.08706848162667313 1107 5 -0.22658293091834736 1107 6 -0.004564281935991232 1107 7 -0.23968222017192176 1107 8 -0.04810476431889299 1107 9 -0.16085569717662906 1107 10-11 -0.227211122319126 1107 12-13 -0.20817849510164166 1107 14-15 -0.2719296640921769 1107 16-17 -0.29320716269531033 1107 18-19 -0.1659719545366798 1107 20-21 -0.08026876792971649 1107 22-23 -0.16146863554521218 1107 24-25 -0.3072305047959887 1107 26-27 -0.23273187329338896 1107 28-29 -0.2119128091442164 1107 30-31 -0.24805621008013645 1107 32-33 -0.2019593001689728 1107 34-35 -0.1128533318999132 1107 36-37 0.11270834634938609 1107 38-39 0.06560383841736694 1107 40-41 0.12617304042509403 1107 42-43 0.26261399334686075 1107 44-45 0.3328103313254118 1107 46-47 0.07331109877910791 1107 48-49 -0.08340515613395638 1107 50-51 0.20291799464860816 1107 52-53 0.18164728777524886 1107 54-55 0.011221928271311299 1107 56-57 -0.3252677812661844 1107 58-59 -0.11178615486502252 1107 60-61 -0.010391084090549896 1107 62-63 -0.2196438930231963 1107 64-65 0.08322072368439848 1107 66-67 0.3053168856904449 1107 68-69 0.08486104019103635 1107 70-71 0.039614363649295825 1107 72-73 -0.0182313391128055 1107 74-75 0.37504816933754626 1107 76-77 0.2889670993527247 1107 78-79 0.2831813866858397 1107 80-81 0.18409431882069782 1107 82-83 0.047328387951793616 1107 84-85 0.19255332417631266 1107 86-87 0.14069083210508637 1107 88-89 -0.28350681035984593 1107 90-91 -0.1950358450111871 1107 92-93 -0.17605562693676902 1107 94-95 -0.3308697776876741 1107 96-97 -0.22601745130391393 1107 98-99 -0.20344768739948904 1107 100-101 -0.010525958795419399 1108 1 -0.1991194211282057 1108 2 -0.12117436512624025 1108 3 -0.15541183432196348 1108 4 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-0.6170963108131247 2316 54-55 -0.9980485098107863 2316 56-57 -1.142688244555913 2316 58-59 -1.5086007063018556 2316 60-61 -1.1494741896707197 2316 62-63 -1.0503580650188482 2316 64-65 -0.9490914794625809 2316 66-67 -0.8828812993601076 2316 68-69 -0.9274110960893793 2316 70-71 -1.1758578150860721 2316 72-73 -0.8307495814775443 2316 74-75 -0.9200038379859343 2316 76-77 -0.9928276839943173 2316 78-79 -0.8658355156942861 2316 80-81 -1.0059811714013946 2316 82-83 -0.9496035779781025 2316 84-85 -0.700281074953363 2316 86-87 -0.5129131958090483 2316 88-89 -0.49977833635023927 2316 90-91 -0.9242926179036921 2316 92-93 -0.8179812246229723 2316 94-95 -0.8192444161571757 2316 96-97 -0.9866046564409636 2316 98-99 -1.0646796772102967 2316 100-101 -1.1803723249163625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 15.0 5 27.0 6 88.0 7 198.0 8 465.0 9 748.0 10 1324.0 11 2190.0 12 3541.0 13 4747.0 14 5337.0 15 6569.0 16 8354.0 17 10467.0 18 9428.0 19 6830.0 20 6281.0 21 6122.0 22 6722.0 23 7424.0 24 8360.0 25 10100.0 26 11986.0 27 14938.0 28 18703.0 29 23564.0 30 28861.0 31 35170.0 32 43198.0 33 54028.0 34 69318.0 35 91994.0 36 128743.0 37 174783.0 38 167164.0 39 38825.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.136720108938746 20.72350830459113 14.195135527348993 19.944636059121134 2 15.365289091992848 23.681402741903437 38.8388635008941 22.114444665209618 3 21.685140113633313 27.413217202219396 29.615620456381762 21.286022227765528 4 12.66511629293123 17.823469982784296 40.20964814595898 29.301765578325494 5 11.942612992343829 40.02755704875332 33.86119552292331 14.168634435979532 6 27.463193657984146 40.08940811825713 17.91927440344917 14.528123820309553 7 27.288730120728484 32.994350506984134 22.757314191085385 16.959605181202 8 24.69226803253202 34.15464729659789 20.17287230079502 20.980212370075073 9 24.18285005846187 20.592387141765816 22.714299763867682 32.51046303590463 10-11 24.46722221421976 26.959040247478107 28.40589111539491 20.16784642290722 12-13 27.168081109513935 24.66033006867407 27.911545251078092 20.2600435707339 14-15 22.129589800190143 27.687071518477417 28.238230014295553 21.945108667036887 16-17 19.986807584446858 29.808749710670856 28.951391710434425 21.25305099444786 18-19 19.578071185400127 31.124365338517585 29.96451558105736 19.33304789502493 20-21 21.78279154748504 28.012939672156556 29.35231098928284 20.85195779107556 22-23 20.44148895482889 28.740789895881246 29.40567571890221 21.412045430387654 24-25 21.997856232472614 28.922831322847614 29.454600904594397 19.624711540085375 26-27 21.901024526544553 27.100701645451725 31.594014281279104 19.40425954672462 28-29 20.40581435088779 30.372343384889284 30.10921529510881 19.112626969114118 30-31 21.660640314976433 28.377981653199065 29.523526615379343 20.43785141644516 32-33 21.343701724146495 29.044243655528344 29.914512932340536 19.69754168798462 34-35 22.18408401576438 27.609870322371055 30.59991674722052 19.606128914644046 36-37 22.142105561235592 27.597523570493887 31.089006993896362 19.171363874374162 38-39 21.202550352573578 29.12687432782512 30.743124256416245 18.92745106318506 40-41 20.60918572390171 29.332419281525475 29.837432905368317 20.2209620892045 42-43 20.253288418191627 29.700170567601692 28.724547727636224 21.32199328657046 44-45 21.586636321461818 28.38510131238706 28.74014418277059 21.288118183380536 46-47 22.370714383280973 29.588704970756602 28.400094373595262 19.640486272367163 48-49 22.24034634064446 29.142870482864264 28.65823795819554 19.958545218295733 50-51 20.548428980001766 29.470396040685888 30.2398873876335 19.741287591678844 52-53 21.542628489458483 30.18207122910994 28.184582556408007 20.09071772502357 54-55 20.375824898498866 29.87754299207561 28.323361204960268 21.423270904465255 56-57 21.868132959806083 28.588394826250223 28.764777175100832 20.77869503884286 58-59 23.3310104114254 28.04379345234045 28.200205735050805 20.424990401183347 60-61 21.095691576635673 29.431827382900927 29.761914815088947 19.71056622537446 62-63 20.401442630117387 28.9171332197378 29.804469967560692 20.876954182584115 64-65 20.179775548915238 31.159372395687097 28.619625331774245 20.04122672362342 66-67 20.93821061224003 28.38022266616123 28.195603215240144 22.48596350635859 68-69 20.55999006581398 29.4891841549733 28.053048553334158 21.897777225878553 70-71 22.060147828933285 29.403811604288144 27.758778864068606 20.777261702709964 72-73 21.50820701363647 27.724487129944297 29.780537014759016 20.986768841660215 74-75 20.40448462656424 30.612960554876178 28.402088534221303 20.58046628433828 76-77 21.156790070720486 28.92864274084354 27.93469158258323 21.979875605852744 78-79 21.05382366469012 29.57917386471218 28.00020662819541 21.366795842402293 80-81 21.478603551024747 28.72991211347862 28.04381709828449 21.74766723721215 82-83 21.23964002984188 28.323460545438834 29.15225815808845 21.28464126663084 84-85 20.40867720074148 29.35290541386278 28.233436448852515 22.004980936543227 86-87 20.56200195999835 28.95751035505971 27.288382514589472 23.192105170352466 88-89 20.32039291146081 29.33578969223326 27.30437624610213 23.039441150203796 90-91 21.030826343902827 27.32071775482571 27.80346281040174 23.844993090869714 92-93 21.104387968278598 29.0158636810217 27.61541128448232 22.264337066217383 94-95 21.149271709031307 29.55651860774112 27.318622642212315 21.97558704101526 96-97 19.7556018262808 30.063647634298796 28.49580185886457 21.68494868055583 98-99 20.75433648608778 30.275979428451084 26.509065369404738 22.4606187160564 100-101 18.994584896719648 29.655513600343326 26.57682224533567 24.77307925760136 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 257.0 1 176.5 2 232.5 3 349.5 4 279.5 5 230.0 6 299.0 7 469.5 8 676.0 9 824.0 10 851.5 11 834.0 12 889.5 13 1003.0 14 1124.0 15 1251.0 16 1394.5 17 1543.0 18 1636.0 19 1762.0 20 1970.5 21 2293.0 22 2653.5 23 3134.5 24 3902.5 25 4893.0 26 6218.5 27 7983.5 28 10312.5 29 13157.0 30 15988.0 31 18825.5 32 21496.5 33 23847.5 34 26100.0 35 28139.0 36 30106.0 37 32072.0 38 33603.0 39 34286.5 40 34783.0 41 35267.0 42 35858.5 43 36748.5 44 36862.5 45 36755.0 46 38521.0 47 46700.0 48 67850.0 49 66746.0 50 44632.5 51 34437.0 52 28151.5 53 23327.0 54 19608.0 55 17007.5 56 14690.5 57 12264.5 58 10018.0 59 8138.5 60 6399.5 61 4860.0 62 3583.0 63 2489.5 64 1620.0 65 1016.0 66 605.5 67 330.0 68 179.0 69 102.5 70 62.0 71 33.5 72 18.5 73 12.0 74 8.5 75 6.5 76 3.0 77 2.0 78 2.0 79 1.5 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05652473230224539 2 0.0038742786639500352 3 0.041723000996385 4 0.0 5 0.0 6 0.0018874690926936073 7 0.0 8 0.0 9 0.0 10-11 4.470321535326964E-4 12-13 0.0 14-15 0.003178895314010286 16-17 0.0 18-19 0.0 20-21 0.012119538384664215 22-23 0.0 24-25 0.0 26-27 0.00759954661005584 28-29 0.009437345463468035 30-31 0.008692291874246874 32-33 0.005364385842392357 34-35 0.006556471585146214 36-37 0.01887469092693607 38-39 0.011076463359754589 40-41 0.013063272931011017 42-43 0.0 44-45 0.0 46-47 1.4901071784423215E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0018874690926936073 58-59 4.470321535326964E-4 60-61 0.008692291874246874 62-63 0.014305028913046285 64-65 0.04922320712787802 66-67 0.024288747008609838 68-69 0.0013907666998795 70-71 2.4835119640705355E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 9.934047856282143E-5 86-87 5.463726320955178E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.4901071784423215E-4 96-97 2.980214356884643E-4 98-99 5.960428713769286E-4 100-101 9.934047856282142E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1006639.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.95679346371644 #Duplication Level Percentage of deduplicated Percentage of total 1 85.4481464033053 55.50437597776907 2 9.563434684351682 12.424201031903486 3 2.151035376890723 4.191730821295144 4 0.883676272460332 2.2960310847597034 5 0.4628752776726934 1.503344690562277 6 0.2983841451719451 1.1629246374468971 7 0.2036958541358013 0.9262000668560186 8 0.15717799346792524 0.8167822766989913 9 0.10895045815427656 0.636936516729416 >10 0.6540393714919387 7.657553425504799 >50 0.042351648101226366 1.9047943634021334 >100 0.020403067768102735 2.4829280368470465 >500 0.002147691010313361 0.9200494655064121 >1k 0.0032215365154700418 4.335980980459278 >5k 3.068130014733373E-4 1.0098322629093448 >10k+ 1.5340650073666866E-4 2.2263343613499686 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22342 2.2194649720505564 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5121 0.5087225907202085 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 5013 0.49799381903542383 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3591 0.3567316585190917 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3364 0.33418136988533126 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3039 0.3018957143524143 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2774 0.27557048753326663 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2753 0.2734843374834474 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2631 0.2613647990987832 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2355 0.23394682701544448 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2259 0.22441014107341362 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2160 0.2145754336956943 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2037 0.20235655483246723 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1927 0.1914291021905569 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1884 0.18715746161235558 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1843 0.18308450199127987 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1758 0.17464056131344008 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1655 0.16440849202146945 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1544 0.1533816989009963 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1432 0.1422555653019603 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1295 0.12864591973885375 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1183 0.11751978613981774 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1020 0.10132728813407785 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1009 0.10023454286988683 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.934047856282143E-5 0.0 10-11 0.0 0.0 0.0 1.4901071784423215E-4 0.0 12-13 0.0 0.0 0.0 2.980214356884643E-4 0.0 14-15 0.0 0.0 0.0 5.960428713769285E-4 0.0 16-17 0.0 0.0 0.0 0.001043075024909625 0.0 18-19 0.0 0.0 0.0 0.0014404369391609107 0.0 20-21 0.0 0.0 0.0 0.0018874690926936073 0.0 22-23 0.0 0.0 0.0 0.0034272465104173396 0.0 24-25 0.0 0.0 0.0 0.006308120388739161 0.0 26-27 0.0 0.0 0.0 0.010728771684784715 0.0 28-29 0.0 0.0 0.0 0.019818425473282872 0.0 30-31 0.0 0.0 0.0 0.03869311640021895 0.0 32-33 0.0 0.0 0.0 0.07023371834391476 0.0 34-35 0.0 0.0 0.0 0.0978007011450977 0.0 36-37 0.0 0.0 0.0 0.1228345017429287 0.0 38-39 0.0 0.0 0.0 0.1488617071263879 0.0 40-41 0.0 0.0 0.0 0.19262118793331073 0.0 42-43 0.0 0.0 0.0 0.2761168601653622 0.0 44-45 0.0 0.0 0.0 0.3937359867837427 0.0 46-47 0.0 0.0 0.0 0.5110074217271534 0.0 48-49 0.0 0.0 0.0 0.7417256831893062 0.0 50-51 0.0 0.0 0.0 0.9242141423092092 0.0 52-53 0.0 4.9670239281410716E-5 0.0 1.0915035082090006 0.0 54-55 0.0 9.934047856282143E-5 0.0 1.4685006243549077 0.0 56-57 0.0 9.934047856282143E-5 0.0 1.8203645994244213 0.0 58-59 0.0 9.934047856282143E-5 0.0 2.2066997205552337 0.0 60-61 0.0 9.934047856282143E-5 0.0 2.5642757731421097 0.0 62-63 0.0 9.934047856282143E-5 0.0 3.1769085044390293 0.0 64-65 0.0 9.934047856282143E-5 0.0 3.5402463047825488 0.0 66-67 0.0 9.934047856282143E-5 0.0 4.25678917665618 0.0 68-69 0.0 9.934047856282143E-5 0.0 5.127955503412842 0.0 70-71 0.0 9.934047856282143E-5 0.0 5.403426650467546 0.0 72-73 0.0 9.934047856282143E-5 0.0 5.9466203872490535 0.0 74-75 0.0 9.934047856282143E-5 0.0 6.717303819939422 0.0 76-77 0.0 9.934047856282143E-5 0.0 6.992476945558437 0.0 78-79 0.0 9.934047856282143E-5 0.0 7.230149040519988 0.0 80-81 0.0 9.934047856282143E-5 0.0 7.800015695795613 0.0 82-83 0.0 9.934047856282143E-5 0.0 8.680619368015744 0.0 84-85 0.0 9.934047856282143E-5 0.0 9.909709439034252 0.0 86-87 0.0 9.934047856282143E-5 0.0 11.268190483380835 0.0 88-89 0.0 9.934047856282143E-5 0.0 12.492015509035513 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1350 0.0 52.09524 2 TATAACG 215 0.0 50.83461 2 TACACGG 110 0.0 47.51931 2 ATAACGC 270 0.0 43.99936 3 ACCTGGG 1580 0.0 43.308735 3 TAACGCA 270 0.0 42.21631 4 GTATAAC 375 0.0 39.30216 1 GTACCTG 1890 0.0 37.73249 1 GTACCGG 105 4.6196874E-8 36.22319 1 GTACACG 135 3.0559022E-10 35.21699 1 CCTGGGG 1020 0.0 33.524715 4 TCACGCA 145 2.3899702E-8 29.494745 2 GAGTACG 240 0.0 27.733381 1 TAGCGTG 70 0.008390719 27.143099 9 AGTACGC 165 8.374991E-8 25.919622 2 GTATACG 175 1.4728357E-7 24.450655 1 TATACGG 180 1.9428808E-7 23.759655 2 TACGCAG 205 2.6724592E-8 23.167482 4 TTGTACG 145 2.3338094E-5 22.931238 9 ACACCGC 145 2.3356028E-5 22.92896 6 >>END_MODULE