##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003860_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2277035 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.93229045666843 31.0 31.0 34.0 28.0 34.0 2 30.819465225611378 31.0 31.0 34.0 27.0 34.0 3 30.9229287209024 31.0 31.0 34.0 27.0 34.0 4 34.34167458998215 37.0 35.0 37.0 30.0 37.0 5 34.316443532927686 37.0 35.0 37.0 30.0 37.0 6 34.46154187353291 37.0 35.0 37.0 31.0 37.0 7 34.52184880776975 37.0 35.0 37.0 32.0 37.0 8 34.5084401425538 37.0 35.0 37.0 31.0 37.0 9 35.96913090927456 39.0 35.0 39.0 31.0 39.0 10-11 35.78353055618381 39.0 35.0 39.0 30.0 39.0 12-13 35.66091759678705 39.0 35.0 39.0 30.0 39.0 14-15 36.59733381348991 39.0 36.0 41.0 30.0 41.0 16-17 36.73596738741389 39.0 36.0 41.0 31.0 41.0 18-19 36.750614285682914 39.0 36.0 41.0 30.5 41.0 20-21 36.61471980009091 39.0 36.0 41.0 30.0 41.0 22-23 36.52190414288757 39.0 36.0 41.0 30.0 41.0 24-25 36.27068819759029 39.0 36.0 41.0 29.0 41.0 26-27 36.06539359298385 39.0 35.5 40.5 28.0 41.0 28-29 35.935128138127 39.0 35.0 40.0 27.0 41.0 30-31 35.73194197717646 38.5 35.0 40.0 27.0 41.0 32-33 35.45995011056044 38.0 35.0 40.0 27.0 41.0 34-35 35.36509715485269 38.0 35.0 40.0 26.0 41.0 36-37 35.21109183653303 38.0 34.5 40.0 25.5 41.0 38-39 35.220192487159835 38.0 34.5 40.0 25.5 41.0 40-41 35.429315096166725 39.0 35.0 40.0 26.0 41.0 42-43 35.26859907730887 39.0 35.0 40.0 25.5 41.0 44-45 35.02102866227353 38.0 35.0 40.0 24.0 41.0 46-47 34.72700683125204 38.0 34.0 40.0 23.5 41.0 48-49 34.422673344942 38.0 34.0 40.0 23.0 41.0 50-51 33.59929777100484 37.5 32.5 39.5 22.0 40.5 52-53 33.79931841188211 38.0 33.0 39.5 22.0 40.5 54-55 34.14215789392785 38.0 34.0 40.0 22.0 41.0 56-57 33.77776757933014 38.0 33.0 40.0 19.5 41.0 58-59 33.45613681827464 38.0 32.5 40.0 18.0 41.0 60-61 33.03752401697822 37.5 32.0 40.0 14.0 41.0 62-63 32.88151082438347 37.0 32.0 40.0 13.5 41.0 64-65 32.60214247914503 36.5 31.5 39.5 13.0 41.0 66-67 32.065592316323645 36.0 31.0 39.0 8.0 41.0 68-69 31.603461299453016 35.5 30.5 39.0 7.5 40.0 70-71 31.053857977589274 35.0 30.0 38.5 6.5 40.0 72-73 30.650805762757273 35.0 29.5 37.0 2.0 40.0 74-75 30.32736453326365 35.0 29.0 37.0 2.0 39.0 76-77 29.919950505811286 34.0 29.0 36.5 2.0 39.0 78-79 29.27174571317525 34.0 29.0 36.0 2.0 38.5 80-81 28.667554078000556 34.0 27.0 35.5 2.0 37.0 82-83 28.266887201997335 34.0 26.0 35.0 2.0 37.0 84-85 27.99208488231406 34.0 26.0 35.0 2.0 36.5 86-87 27.942798639458772 34.0 26.0 35.0 2.0 36.0 88-89 27.53493622188504 34.0 26.0 35.0 2.0 36.0 90-91 27.04432935813459 33.0 25.0 35.0 2.0 35.5 92-93 26.735335425235007 33.0 25.0 35.0 2.0 35.0 94-95 26.47273801237135 33.0 24.5 35.0 2.0 35.0 96-97 26.196120173822536 33.0 24.0 35.0 2.0 35.0 98-99 25.977325117971397 33.0 23.5 35.0 2.0 35.0 100-101 25.671317305179763 32.0 21.0 35.0 2.0 35.0 102-103 25.09339821302703 32.0 18.5 35.0 2.0 35.0 104-105 24.638994569692606 32.0 17.5 34.5 2.0 35.0 106-107 24.146698667345913 31.0 10.0 34.0 2.0 35.0 108-109 23.90506799412394 31.0 7.0 34.0 2.0 35.0 110-111 23.557238470203576 31.0 2.0 34.0 2.0 35.0 112-113 22.86497726209742 31.0 2.0 34.0 2.0 35.0 114-115 22.26981469323045 30.5 2.0 34.0 2.0 35.0 116-117 21.54093832549785 29.5 2.0 34.0 2.0 35.0 118-119 20.790071079276338 29.0 2.0 34.0 2.0 35.0 120-121 19.204654737410713 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.37486343313004866 1101 2 0.2561144313784567 1101 3 0.3966003331538204 1101 4 0.5829494252363432 1101 5 0.5115781594682787 1101 6 0.5585464416942898 1101 7 0.44235630212900645 1101 8 0.40059749498129804 1101 9 0.4498625764600632 1101 10-11 0.6337300807434119 1101 12-13 0.6799967825858175 1101 14-15 0.7873343784765794 1101 16-17 0.6660559708562559 1101 18-19 0.5484971270408749 1101 20-21 0.6537567402628497 1101 22-23 0.5523053180165434 1101 24-25 0.5322986328579731 1101 26-27 0.6016090252871322 1101 28-29 0.6604395119320401 1101 30-31 0.7843297962742994 1101 32-33 0.5772823044192137 1101 34-35 0.6794455432620339 1101 36-37 0.7906287868008164 1101 38-39 0.7503745362280654 1101 40-41 0.7679428294657598 1101 42-43 0.8512017600108095 1101 44-45 0.8699459975300314 1101 46-47 0.7287555116544056 1101 48-49 0.8278818451356855 1101 50-51 0.5257195972243451 1101 52-53 0.392103432291691 1101 54-55 0.7298605526577759 1101 56-57 0.4790390539131053 1101 58-59 0.48375122879188126 1101 60-61 0.5001285061798129 1101 62-63 0.5306216647399324 1101 64-65 0.49388829138868573 1101 66-67 0.470383453711932 1101 68-69 0.39084316368868244 1101 70-71 0.17787389601809522 1101 72-73 0.1579977090353708 1101 74-75 0.04227627520893407 1101 76-77 0.10419901529665765 1101 78-79 0.3397953365007105 1101 80-81 0.4377032847557132 1101 82-83 0.47867708052024227 1101 84-85 0.5078158041633856 1101 86-87 0.40797592179723097 1101 88-89 0.48629494459580513 1101 90-91 0.4895298104260455 1101 92-93 0.5713953718466769 1101 94-95 0.42930231958615295 1101 96-97 0.3433172874070394 1101 98-99 0.3445465417703346 1101 100-101 0.2827631972159317 1101 102-103 0.1725638102866469 1101 104-105 0.2055261157282331 1101 106-107 0.05164048795829501 1101 108-109 0.35608179557240405 1101 110-111 0.19717933659327613 1101 112-113 0.34498861023588034 1101 114-115 0.23928879192485297 1101 116-117 -0.03683707072692499 1101 118-119 -0.08539605594299715 1101 120-121 0.03711499832860099 1102 1 0.4569921082518036 1102 2 0.43141423211078234 1102 3 0.4645034905033434 1102 4 0.7108012190050914 1102 5 0.8466724699086043 1102 6 0.8247774051570929 1102 7 0.5848611039763725 1102 8 0.6542116494605068 1102 9 0.6599493334326354 1102 10-11 0.6613721758595617 1102 12-13 0.8155270360226439 1102 14-15 0.770147002119657 1102 16-17 0.7410119988832164 1102 18-19 0.7575174962256028 1102 20-21 0.8778738837571609 1102 22-23 0.7915176172201939 1102 24-25 0.7741324349858942 1102 26-27 0.9316453898390691 1102 28-29 1.0433986188963331 1102 30-31 0.8961528616873338 1102 32-33 0.6140631063816784 1102 34-35 0.8112338627233626 1102 36-37 0.7194957126833188 1102 38-39 0.5681433497581239 1102 40-41 0.5912056218029491 1102 42-43 0.7157102630573746 1102 44-45 0.7560484261497606 1102 46-47 0.7494939170813879 1102 48-49 0.6637127985271931 1102 50-51 0.2655672869422787 1102 52-53 0.3144325620602686 1102 54-55 0.48689579857710896 1102 56-57 0.4131607654569649 1102 58-59 0.44169441952053745 1102 60-61 0.4420702149900322 1102 62-63 0.36517806036899714 1102 64-65 0.31075923139976425 1102 66-67 0.13179143808704907 1102 68-69 0.06378619255731977 1102 70-71 -0.09239547502982148 1102 72-73 0.07938598245651463 1102 74-75 -0.014484887737346241 1102 76-77 -0.12463059285820677 1102 78-79 0.04208853334408147 1102 80-81 0.08992031746582185 1102 82-83 0.13015027650035904 1102 84-85 -0.09504471206218312 1102 86-87 -0.08140435214186326 1102 88-89 -0.03868347406773154 1102 90-91 0.11544791644795538 1102 92-93 0.0852317961069744 1102 94-95 -0.06319546832685674 1102 96-97 0.021315294730307244 1102 98-99 0.19433135786137967 1102 100-101 0.21034828163492847 1102 102-103 -0.021749597478315508 1102 104-105 0.07453441734899258 1102 106-107 -0.019151780887607828 1102 108-109 -0.12392102798578364 1102 110-111 -0.22084080958430974 1102 112-113 -0.27897827320331814 1102 114-115 -0.11610348483420552 1102 116-117 -0.10742170913163207 1102 118-119 -0.16799196699911434 1102 120-121 -0.15776105286233033 1103 1 0.6703312085978226 1103 2 0.6474676514957203 1103 3 0.7825303945894966 1103 4 0.9278367152921732 1103 5 1.0460657672143086 1103 6 1.010080464913706 1103 7 0.8251655244887885 1103 8 0.7448578140730788 1103 9 0.8978253880183829 1103 10-11 1.0326718920447817 1103 12-13 1.0472060142894364 1103 14-15 1.210803275203908 1103 16-17 1.0591317259544795 1103 18-19 0.9179470090750286 1103 20-21 0.9016513418653247 1103 22-23 1.024244863439236 1103 24-25 0.9748892096058555 1103 26-27 1.1652138747346825 1103 28-29 1.1558521588053523 1103 30-31 0.9944580403351253 1103 32-33 1.0430268908379645 1103 34-35 0.9358743316009352 1103 36-37 0.8762383548822967 1103 38-39 0.7946063566396546 1103 40-41 1.0459423155551377 1103 42-43 0.9911092560980777 1103 44-45 1.0627568550599875 1103 46-47 1.0937983764725416 1103 48-49 1.0552681424096164 1103 50-51 1.0089478903402878 1103 52-53 0.9782142968295204 1103 54-55 0.8903959540920567 1103 56-57 0.944307105023853 1103 58-59 0.9211615864269547 1103 60-61 0.9618052563958841 1103 62-63 1.0510444730279147 1103 64-65 0.9615553707411593 1103 66-67 1.0198291310227816 1103 68-69 0.8518023077938928 1103 70-71 0.8319330891784134 1103 72-73 0.6288201996331395 1103 74-75 0.4466197543838746 1103 76-77 0.62758802228117 1103 78-79 0.7687929771837467 1103 80-81 0.7195234627556637 1103 82-83 0.7232999401992828 1103 84-85 0.6431574379319827 1103 86-87 0.7159667648442571 1103 88-89 0.7322245388400077 1103 90-91 0.6708108494598939 1103 92-93 0.4664281920480349 1103 94-95 0.5383303277152827 1103 96-97 0.5487596009392384 1103 98-99 0.4786407742915344 1103 100-101 0.5750372517371822 1103 102-103 0.6536126551557153 1103 104-105 0.6748386434677265 1103 106-107 0.5403638872434904 1103 108-109 0.6213591905127203 1103 110-111 0.7917388918269914 1103 112-113 0.595221505187876 1103 114-115 0.6734531031678159 1103 116-117 0.5211549402987927 1103 118-119 0.2902660711798042 1103 120-121 0.45324104658947917 1104 1 0.5472570675489976 1104 2 0.7011336612078125 1104 3 0.673946165562942 1104 4 0.8324074936817496 1104 5 1.0140302916672468 1104 6 0.752189695464125 1104 7 0.6385584578248356 1104 8 0.6913901098245461 1104 9 0.8081141093951416 1104 10-11 0.919565140312649 1104 12-13 0.9887830100429298 1104 14-15 1.1752415790420088 1104 16-17 1.0667568351647105 1104 18-19 0.950919559210142 1104 20-21 0.936168683434289 1104 22-23 0.9546991780053418 1104 24-25 1.0351817914373314 1104 26-27 1.0478869348561801 1104 28-29 1.004802521484919 1104 30-31 0.9420800002270369 1104 32-33 0.9664094619008097 1104 34-35 1.1440086144706711 1104 36-37 1.079295123884883 1104 38-39 1.0124155485855297 1104 40-41 1.0864427523960671 1104 42-43 1.023728574626233 1104 44-45 1.0658742738380553 1104 46-47 1.2544550601387598 1104 48-49 1.2186615837002321 1104 50-51 0.9867988361516851 1104 52-53 1.0090316973212197 1104 54-55 1.14355583787205 1104 56-57 1.001482877622756 1104 58-59 0.885477777986786 1104 60-61 0.7235601378392928 1104 62-63 0.8680062951625374 1104 64-65 0.784939465667712 1104 66-67 0.6953262153635578 1104 68-69 0.7789920999388933 1104 70-71 0.6789359540421778 1104 72-73 0.6752092521962823 1104 74-75 0.7417053955241322 1104 76-77 0.5958966351588337 1104 78-79 0.7176291516763023 1104 80-81 0.9309764160035279 1104 82-83 1.027340109246559 1104 84-85 0.9544630843549697 1104 86-87 1.1175837874177539 1104 88-89 1.077927243190132 1104 90-91 1.1873324781654233 1104 92-93 1.2346788981048888 1104 94-95 1.2552789430311329 1104 96-97 1.136457144470949 1104 98-99 1.1142683013655095 1104 100-101 1.101616888854437 1104 102-103 1.1925756151085807 1104 104-105 1.0728621922415265 1104 106-107 0.9734057223817558 1104 108-109 0.9807709791223473 1104 110-111 1.017644066868133 1104 112-113 0.9959248190834806 1104 114-115 0.8890062656929594 1104 116-117 0.5592517361213751 1104 118-119 0.5836106472409206 1104 120-121 0.6874275065524991 1105 1 0.6831383126925452 1105 2 0.911268807795782 1105 3 0.831360181233844 1105 4 1.0161935607991737 1105 5 1.1850825241418548 1105 6 1.0092088607829552 1105 7 1.0733755875143487 1105 8 0.9009212941704163 1105 9 0.9208079326545615 1105 10-11 1.0818334913529881 1105 12-13 1.1824808157062634 1105 14-15 1.4088728195019442 1105 16-17 1.380262416685781 1105 18-19 1.1525941047441677 1105 20-21 1.3887777200297649 1105 22-23 1.1447017294558748 1105 24-25 1.446188356515826 1105 26-27 1.3849063410462108 1105 28-29 1.3928596365080566 1105 30-31 1.342383185704108 1105 32-33 1.1998484514550256 1105 34-35 1.1614946372499517 1105 36-37 1.2648570585623915 1105 38-39 1.0731042751886832 1105 40-41 1.0951518076367535 1105 42-43 1.2523698781498283 1105 44-45 1.2505620467696659 1105 46-47 1.4349532353163568 1105 48-49 1.3985342743475613 1105 50-51 1.0530921673201519 1105 52-53 1.084482817862991 1105 54-55 1.3015929962698465 1105 56-57 1.4392832417843309 1105 58-59 1.405976140508649 1105 60-61 1.3234558941347245 1105 62-63 1.3135647193652815 1105 64-65 1.321502710863001 1105 66-67 1.3115452333048978 1105 68-69 1.3449288079075323 1105 70-71 1.0296457657968752 1105 72-73 0.847646494096093 1105 74-75 1.027448898023394 1105 76-77 1.1461995753041272 1105 78-79 1.216554821498974 1105 80-81 1.2990771287146572 1105 82-83 1.244562779509046 1105 84-85 1.298878351664019 1105 86-87 1.1620643352837696 1105 88-89 1.172188397534157 1105 90-91 1.1725025064722487 1105 92-93 1.2985738880730509 1105 94-95 1.2553917956509615 1105 96-97 1.1188867052556866 1105 98-99 1.327454929266782 1105 100-101 1.313671199066217 1105 102-103 1.293506448496185 1105 104-105 1.5119705093457902 1105 106-107 1.4317885309578422 1105 108-109 1.4716015922466141 1105 110-111 1.4647321347331506 1105 112-113 1.407920628816644 1105 114-115 1.4482097313835318 1105 116-117 1.4598662699329985 1105 118-119 1.43600566531072 1105 120-121 1.415900869319703 1106 1 0.5297603424560897 1106 2 0.7340393581222813 1106 3 0.7426551402322481 1106 4 0.9432719979604869 1106 5 0.9661347754422138 1106 6 0.9802994333291721 1106 7 0.8566031959034603 1106 8 0.8879067833732677 1106 9 0.9720917332364678 1106 10-11 1.0045040383776112 1106 12-13 1.0693056780954961 1106 14-15 1.1253112598097559 1106 16-17 0.9558905510451652 1106 18-19 0.9905170126139069 1106 20-21 1.1014736235604516 1106 22-23 0.9455930674558672 1106 24-25 1.196492323823314 1106 26-27 1.2072623353203653 1106 28-29 1.2517407503297378 1106 30-31 1.138404006007967 1106 32-33 1.0630258955269838 1106 34-35 1.040748480057097 1106 36-37 1.078035886511067 1106 38-39 1.07594073689269 1106 40-41 0.9950937576501602 1106 42-43 0.9894515103668553 1106 44-45 0.9904664233162066 1106 46-47 1.0171746924948408 1106 48-49 1.0854307098695841 1106 50-51 0.791265969611274 1106 52-53 0.6530492963871666 1106 54-55 0.9457981703178362 1106 56-57 0.8310898175780395 1106 58-59 1.0459348767038747 1106 60-61 0.8226981609345074 1106 62-63 0.8284738059483701 1106 64-65 0.8766955989991416 1106 66-67 0.8879966529196253 1106 68-69 0.9566500252140493 1106 70-71 0.5955072509053885 1106 72-73 0.7727378542381125 1106 74-75 0.7528084874635894 1106 76-77 0.9158012178929553 1106 78-79 0.9504194259141734 1106 80-81 0.8516649296228671 1106 82-83 1.0755907037133916 1106 84-85 1.147145980796708 1106 86-87 1.0760035913504922 1106 88-89 0.9394858853851744 1106 90-91 1.0263850333048907 1106 92-93 0.9533496951887983 1106 94-95 0.9190403162139127 1106 96-97 1.1649386020893786 1106 98-99 1.2928655376286997 1106 100-101 1.1536362962386413 1106 102-103 1.0906739441940267 1106 104-105 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-0.19493979380983362 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 32842.0 3 4616.0 4 4075.0 5 4631.0 6 5561.0 7 5643.0 8 7145.0 9 8763.0 10 11384.0 11 14266.0 12 15278.0 13 17593.0 14 21866.0 15 22566.0 16 18396.0 17 18740.0 18 20187.0 19 21662.0 20 24073.0 21 26854.0 22 29113.0 23 32423.0 24 36332.0 25 40781.0 26 45695.0 27 53040.0 28 61070.0 29 71831.0 30 84753.0 31 100418.0 32 121684.0 33 147812.0 34 183104.0 35 231463.0 36 297559.0 37 318886.0 38 114290.0 39 640.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.76618534411437 17.86822278358135 13.585386032453423 20.78020583985086 2 13.578799707029345 22.70070351972675 37.057374011091035 26.663122762152874 3 24.774578452728313 23.963960928261923 30.906106610636236 20.355354008373528 4 10.835034432010355 15.398145747031666 43.97655919269292 29.79026062826506 5 10.423583455654008 39.46352390827966 35.932486967361996 14.180405668704335 6 25.792650242540134 37.09678062942058 18.367122544283916 18.743446583755375 7 24.055450561295764 33.258192507073815 24.380863821066143 18.305493110564274 8 21.82822170174012 28.215142779271023 21.438052165897393 28.518583353091465 9 20.42523697275805 21.254314789874332 27.80428658226098 30.516161655106643 10-11 24.519463519375115 23.136851392349925 28.510874759961634 23.83281032831333 12-13 29.364274504442804 22.213800112690336 27.213652768856523 21.208272614010337 14-15 23.172068693826436 24.70132348262137 31.5282754488789 20.598332374673294 16-17 19.098203374639805 28.643238242903678 30.793156042184993 21.465402340271524 18-19 17.841486551856082 32.50060854840919 29.203029928166902 20.454874971567822 20-21 20.831218209410345 27.217178190452923 26.49344004937087 25.458163550765857 22-23 18.65866366332425 27.07963511339878 29.115667244198423 25.146033979078545 24-25 23.66458851044197 26.20391459216683 27.777223780063792 22.35427311732741 26-27 21.84725980193368 23.461994446697382 34.015285986220896 20.67545976514804 28-29 18.40719203928492 30.80432790184533 29.96725148531969 20.82122857355006 30-31 19.797735205308253 26.643235208189896 28.789306175539984 24.76972341096186 32-33 21.092821999716726 26.48961587250853 30.54139498429475 21.87616714347999 34-35 24.224332776778265 24.670766494261688 29.319915124613065 21.784985604346982 36-37 22.15424254798963 24.11125018899665 33.37807177749075 20.356435485522965 38-39 21.186213940914293 27.628820092429894 31.43977438044063 19.74519158621518 40-41 19.965439690708507 29.450814738785912 30.161351642833946 20.422393927671635 42-43 18.631556804078304 29.248527083291307 26.578690293922776 25.54122581870761 44-45 20.513344478573632 26.15603794464511 27.13643640286963 26.19418117391163 46-47 23.687908086082643 28.089819162266394 28.265076496679463 19.9571962549715 48-49 22.302332390140826 26.65638441296628 29.274990298053115 21.76629289883978 50-51 17.475244715059265 28.06862663372465 33.566756352659276 20.889372298556808 52-53 20.033604922516073 30.173128961383593 27.587287578302327 22.205978537798003 54-55 18.45289896353647 26.684624527464578 29.70721091161709 25.155265597381867 56-57 22.475898949019413 24.664465997712984 28.496637341170818 24.36299771209679 58-59 25.7833221157811 24.86640915068498 27.580413887773048 21.769854845760875 60-61 22.326180769472106 26.062058951281013 32.576348669251516 19.035411609995364 62-63 19.733317492358985 27.75332056450317 31.233066910901393 21.280295032236456 64-65 18.420580787792485 31.96458313709628 28.48278193030768 21.13205414480355 66-67 20.22963371596015 27.34010493627824 26.31753807336674 26.112723274394874 68-69 18.763688615151068 27.837123435618512 28.576308653776238 24.822879295454182 70-71 23.30162194084895 26.594429660475132 27.016474790351886 23.08747360832403 72-73 21.446913736803726 24.155592687438105 32.757237373158496 21.64025620259967 74-75 18.447751796368024 30.75459650725309 29.308633873481593 21.489017822897292 76-77 19.856768696833726 26.564932144957282 28.567012896937143 25.01128626127185 78-79 21.058332370064672 26.79996086281952 29.65417952473175 22.487527242384058 80-81 23.614075736088825 25.680583944197732 27.88067227263749 22.824668047075953 82-83 21.125768195433444 24.715338973237007 32.56110400959082 21.597788821738728 84-85 20.73829410023317 27.61296216835917 30.49032228020982 21.158421451197835 86-87 20.220515282090396 29.294410959693774 28.614507315959198 21.870566442256635 88-89 19.052544109711253 28.059789050247225 26.228866336855372 26.658800503186153 90-91 20.03200945826719 24.638391349552553 27.983197916946146 27.34640127523411 92-93 22.89621038516931 26.499235549942888 29.131178565600813 21.473375499286988 94-95 20.9750073990159 25.477666056640437 29.757944104453042 23.789382439890616 96-97 16.329306144059576 26.81928022315348 33.567584477964566 23.28382915482237 98-99 19.009863922493885 28.821177646862083 28.890287649315088 23.278670781328945 100-101 17.17955541362854 25.9053612929551 30.386063654265794 26.52901963915057 102-103 20.507327347300745 24.60538742933156 29.421993127147765 25.465292096219933 104-105 23.485395145008837 24.634798263658194 28.03193091876211 23.847875672570858 106-107 20.472485108062152 25.445117639104108 32.874171715626616 21.20822553720712 108-109 18.82182267683977 26.376387238854594 31.277149822572202 23.524640261733435 110-111 18.385850135525207 29.4039928006599 28.428260092232954 23.781896971581936 112-113 20.344082207269622 25.48531775504096 26.39451951535844 27.776080522330982 114-115 18.763441003252 25.53710803263176 28.924706437403614 26.774744526712624 116-117 22.629814248358855 24.76995329319459 27.050494401104586 25.54973805734197 118-119 20.94389903098467 23.358553854894517 31.197184207380857 24.50036290673995 120-121 18.732523769447226 29.184872690122322 27.935075554073553 24.147527986356902 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5761.0 1 6431.5 2 4442.0 3 1693.5 4 1502.0 5 1409.0 6 1456.5 7 1583.5 8 1790.0 9 1924.0 10 1839.5 11 1741.5 12 1774.5 13 1893.5 14 2109.5 15 2269.5 16 2396.0 17 2623.0 18 2908.5 19 3208.0 20 3562.0 21 4061.5 22 4784.0 23 5724.0 24 7070.0 25 8882.0 26 11229.5 27 14230.0 28 18203.0 29 22898.5 30 27754.5 31 32527.5 32 36870.0 33 40643.0 34 43986.0 35 47599.0 36 51456.5 37 55694.5 38 59314.5 39 60737.0 40 61873.5 41 63067.0 42 64126.5 43 66529.0 44 67749.5 45 70693.0 46 83687.0 47 144883.5 48 275300.0 49 270769.0 50 140172.5 51 77025.0 52 54141.5 53 43975.0 54 37863.0 55 32295.0 56 26901.5 57 21694.5 58 17280.0 59 13918.5 60 11065.5 61 8412.0 62 6148.0 63 4391.0 64 2958.5 65 1868.0 66 1135.0 67 684.5 68 411.0 69 260.0 70 167.0 71 115.5 72 78.5 73 56.5 74 39.0 75 28.5 76 22.5 77 19.5 78 18.0 79 14.5 80 15.5 81 12.0 82 9.0 83 10.5 84 6.0 85 5.0 86 5.0 87 5.0 88 5.5 89 3.0 90 2.0 91 1.5 92 1.5 93 2.0 94 3.0 95 3.5 96 2.0 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.470348501450351 2 0.9450447621578061 3 0.9483385191707638 4 0.9284003100523267 5 0.9377106632089538 6 0.945615680040052 7 0.9146543641182503 8 0.9038069243555765 9 0.9025772550707389 10-11 0.8943428625383448 12-13 0.8962751999859466 14-15 0.8964728254067241 16-17 0.9210222943433016 18-19 0.9515883594235487 20-21 0.9421901727465761 22-23 0.9170697859277526 24-25 0.9094282696576907 26-27 0.909955270779764 28-29 0.9313646913639888 30-31 0.9388524989734458 32-33 0.9336922796531455 34-35 0.9439029263933141 36-37 0.9531693627897683 38-39 0.9389183741137048 40-41 0.9467355574244577 42-43 0.9394453752357781 44-45 0.9250845946592827 46-47 0.9189142898550088 48-49 0.9242062594558275 50-51 0.9555847845992705 52-53 0.9273243494280939 54-55 0.9391159995344823 56-57 0.9911573603392131 58-59 0.9504026068988838 60-61 0.9648292626156383 62-63 0.9346145316167737 64-65 0.9243380097363456 66-67 0.9264020974644658 68-69 0.9446934280764239 70-71 0.9481189353698998 72-73 0.9534548217308912 74-75 0.9569462041646264 76-77 0.969725981374902 78-79 1.0286842319068437 80-81 1.0575155849602664 82-83 1.0194836706506487 84-85 0.9768185381428041 86-87 0.960130169277152 88-89 0.9576488723273907 90-91 0.9695283559541246 92-93 0.9582197902096367 94-95 0.9515664010434622 96-97 0.9620185899645811 98-99 0.9950659519945895 100-101 0.9817591736622406 102-103 0.9567266203637625 104-105 0.948755728392405 106-107 0.9494803549352557 108-109 0.9490631457136144 110-111 0.9464281401032483 112-113 0.9556287013594433 114-115 0.9577806226079089 116-117 0.9854262231366668 118-119 0.9803757957167983 120-121 0.9586589578113643 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2277035.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.024472266504226 #Duplication Level Percentage of deduplicated Percentage of total 1 83.20436171353133 42.45458646704266 2 11.03282782828239 11.258884350906218 3 2.5265568978195923 3.8674869708762225 4 0.9931561700832228 2.0270107782687585 5 0.5569071219153275 1.420794599859366 6 0.34811733397569816 1.0657501951759443 7 0.22872483337776872 0.8169394742141333 8 0.16400945766882113 0.6694796819413731 9 0.13128512315545515 0.6028878712905101 >10 0.7259897240781341 6.8452065869990655 >50 0.051601375983920214 1.7987623951732017 >100 0.02832124708975074 2.7920335499386684 >500 0.0026547303386804835 0.9826445323522883 >1k 0.0035396404515739784 3.917529180741699 >5k 7.079280903147956E-4 2.5237750372573244 >10k+ 0.0012388741580508923 16.95622832796255 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 74575 3.2750923898842133 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 73701 3.236709141493214 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 44069 1.9353677040537365 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 37147 1.6313758901378328 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 23122 1.0154433287147542 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 20813 0.9140395294758316 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 16792 0.7374502368211292 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 15809 0.6942800615712977 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 13627 0.5984536908743168 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 13102 0.5753973917836134 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 11456 0.5031104045392363 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10851 0.4765407646347114 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 10327 0.4535283823041807 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 10145 0.44553553195273676 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 9415 0.41347629702661576 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7990 0.3508949137804206 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 7644 0.33569971476064264 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 7246 0.3182208442118808 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7092 0.31145766314527445 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5681 0.24949111454149803 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5558 0.24408935304024748 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 5269 0.23139740935031744 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4709 0.206804023653567 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4524 0.19867942302160485 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4222 0.18541656144942875 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3777 0.16587360317254676 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3745 0.16446826684701815 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3653 0.16042792491112345 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3575 0.1570024176176475 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3236 0.14211463591907897 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3195 0.14031404875199546 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3173 0.13934788002819457 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3124 0.1371959587797289 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2957 0.12986185983087656 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2623 0.11519366193317186 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2548 0.11189990492021422 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 4.3916760172768534E-5 8 0.0 0.0 0.0 0.0 4.3916760172768534E-5 9 0.0 0.0 0.0 0.0 4.3916760172768534E-5 10-11 0.0 0.0 0.0 4.3916760172768534E-5 4.3916760172768534E-5 12-13 0.0 0.0 0.0 8.783352034553707E-5 4.3916760172768534E-5 14-15 0.0 0.0 0.0 1.0979190043192133E-4 4.3916760172768534E-5 16-17 0.0 0.0 0.0 2.635005610366112E-4 4.3916760172768534E-5 18-19 0.0 0.0 0.0 5.709178822459909E-4 4.3916760172768534E-5 20-21 0.0 0.0 0.0 0.0010540022441464449 4.3916760172768534E-5 22-23 0.0 0.0 0.0 0.0022617131488975794 4.3916760172768534E-5 24-25 0.0 0.0 0.0 0.0042160089765857795 4.3916760172768534E-5 26-27 0.0 0.0 0.0 0.00720234866833404 4.3916760172768534E-5 28-29 0.0 0.0 0.0 0.016798160766083963 4.3916760172768534E-5 30-31 0.0 0.0 0.0 0.040886503720847504 4.3916760172768534E-5 32-33 0.0 0.0 0.0 0.09200561256195008 4.3916760172768534E-5 34-35 0.0 0.0 0.0 0.12946660898932164 4.3916760172768534E-5 36-37 0.0 0.0 0.0 0.14549622645238214 4.3916760172768534E-5 38-39 0.0 0.0 0.0 0.16014246597000048 4.3916760172768534E-5 40-41 0.0 0.0 0.0 0.18574593715072452 4.3916760172768534E-5 42-43 0.0 0.0 0.0 0.213259787398964 4.3916760172768534E-5 44-45 0.0 0.0 0.0 0.2388193418195153 4.3916760172768534E-5 46-47 0.0 0.0 0.0 0.2684411965560477 4.3916760172768534E-5 48-49 0.0 0.0 0.0 0.30058826500251423 4.3916760172768534E-5 50-51 0.0 0.0 0.0 0.3423750623069035 4.3916760172768534E-5 52-53 0.0 0.0 0.0 0.444745030269627 4.3916760172768534E-5 54-55 0.0 0.0 0.0 0.5588846899586524 4.3916760172768534E-5 56-57 0.0 0.0 0.0 0.7120004743010099 4.3916760172768534E-5 58-59 0.0 2.1958380086384267E-5 0.0 0.9519396935049307 4.3916760172768534E-5 60-61 0.0 8.783352034553707E-5 0.0 1.2311185379232203 4.3916760172768534E-5 62-63 0.0 8.783352034553707E-5 0.0 1.360585146912542 4.3916760172768534E-5 64-65 0.0 8.783352034553707E-5 0.0 1.5064985825865653 4.3916760172768534E-5 66-67 0.0 8.783352034553707E-5 0.0 2.035410083727303 4.3916760172768534E-5 68-69 0.0 8.783352034553707E-5 0.0 2.5486872182465357 4.3916760172768534E-5 70-71 0.0 8.783352034553707E-5 0.0 2.8472992290412753 4.3916760172768534E-5 72-73 0.0 8.783352034553707E-5 0.0 3.017125340629371 4.3916760172768534E-5 74-75 0.0 8.783352034553707E-5 0.0 3.689710522675321 4.3916760172768534E-5 76-77 0.0 8.783352034553707E-5 0.0 3.86498231252484 4.3916760172768534E-5 78-79 0.0 1.0979190043192133E-4 0.0 3.9929118349081154 4.3916760172768534E-5 80-81 0.0 1.317502805183056E-4 0.0 4.214867140821287 4.3916760172768534E-5 82-83 0.0 1.317502805183056E-4 0.0 4.723708682563069 4.3916760172768534E-5 84-85 0.0 1.317502805183056E-4 0.0 5.171747469845655 4.3916760172768534E-5 86-87 0.0 1.317502805183056E-4 0.0 6.097381024007097 4.3916760172768534E-5 88-89 0.0 1.317502805183056E-4 0.0 7.334055032092172 4.3916760172768534E-5 90-91 0.0 1.317502805183056E-4 0.0 8.759307608359116 4.3916760172768534E-5 92-93 0.0 1.317502805183056E-4 0.0 10.101623383039787 4.3916760172768534E-5 94-95 0.0 1.317502805183056E-4 0.0 11.167395318912533 4.3916760172768534E-5 96-97 0.0 1.317502805183056E-4 0.0 12.202096146963047 4.3916760172768534E-5 98-99 0.0 1.317502805183056E-4 0.0 13.227376829956501 4.3916760172768534E-5 100-101 0.0 1.317502805183056E-4 0.0 14.082392233760132 4.3916760172768534E-5 102-103 0.0 1.317502805183056E-4 0.0 14.878295678371217 4.3916760172768534E-5 104-105 0.0 1.317502805183056E-4 0.0 15.737834508472641 4.3916760172768534E-5 106-107 0.0 1.317502805183056E-4 0.0 16.74541673711647 4.3916760172768534E-5 108-109 0.0 1.537086606046899E-4 0.0 17.795817806928746 4.3916760172768534E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2450 0.0 54.94215 2 TATAACG 380 0.0 45.414394 2 ACCTGGG 2870 0.0 45.05792 3 GTACCTG 3590 0.0 38.86867 1 ATAACGC 565 0.0 35.603287 3 AGTACGC 1315 0.0 33.24633 2 CCTGGGG 1965 0.0 31.876944 4 GTACGCA 1325 0.0 30.797323 3 GAGTACG 1510 0.0 30.166775 1 TAACGCA 590 0.0 29.220118 4 TACGCAG 1500 0.0 27.200686 4 TATCACG 315 0.0 25.566624 2 CGCAGTG 1740 0.0 24.771578 6 GTATAAC 770 0.0 24.711662 1 ACGCAGT 1700 0.0 24.67832 5 TACACGG 260 1.6370905E-9 24.337458 2 GTACACG 365 0.0 23.696115 1 GTACCGG 195 1.9264553E-6 23.65561 1 TCACGCA 320 3.092282E-11 23.345657 4 GTAGGCA 1125 0.0 21.967794 3 >>END_MODULE