##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003860_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2277035 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.64513457193236 31.0 31.0 34.0 30.0 34.0 2 31.840237414005493 31.0 31.0 34.0 30.0 34.0 3 31.904108193330362 31.0 31.0 34.0 30.0 34.0 4 35.56782175065381 37.0 35.0 37.0 33.0 37.0 5 35.48342296012139 37.0 35.0 37.0 33.0 37.0 6 35.56347882224033 37.0 35.0 37.0 33.0 37.0 7 35.50539363690062 37.0 35.0 37.0 33.0 37.0 8 35.52204994653135 37.0 35.0 37.0 33.0 37.0 9 37.06340657916984 39.0 37.0 39.0 33.0 39.0 10-11 36.9613372214305 39.0 37.0 39.0 33.0 39.0 12-13 36.947017283440964 39.0 37.0 39.0 33.0 39.0 14-15 37.8965703206143 39.5 37.0 41.0 33.0 41.0 16-17 38.011703157834646 40.0 37.0 41.0 33.0 41.0 18-19 38.08332963700602 40.0 37.0 41.0 33.0 41.0 20-21 37.99507495493042 40.0 37.0 41.0 33.0 41.0 22-23 37.89695920352564 40.0 37.0 41.0 33.0 41.0 24-25 37.72234418882451 39.5 36.5 41.0 32.0 41.0 26-27 37.41503402450994 39.0 36.5 41.0 31.5 41.0 28-29 37.315923778071046 39.0 36.0 41.0 31.5 41.0 30-31 37.156533386619 39.0 36.0 40.0 31.0 41.0 32-33 36.88868023548167 39.0 35.0 40.0 31.0 41.0 34-35 36.77590880245583 39.0 35.0 40.0 30.0 41.0 36-37 36.54403599417664 38.5 35.0 40.0 30.0 41.0 38-39 36.31285158111316 38.0 35.0 40.0 30.0 41.0 40-41 36.60624298704236 39.0 35.0 40.0 30.0 41.0 42-43 36.456960037944086 39.0 35.0 40.5 30.0 41.0 44-45 36.290803171668415 39.0 35.0 40.5 30.0 41.0 46-47 36.015958033143974 39.0 35.0 40.0 29.5 41.0 48-49 35.69285101019528 39.0 35.0 40.0 28.0 41.0 50-51 35.33554973902466 38.0 34.0 40.0 27.0 41.0 52-53 35.29101858337707 38.0 34.0 40.0 27.0 41.0 54-55 35.11676917570437 38.0 34.0 40.0 26.5 41.0 56-57 34.72292389005878 38.0 33.5 40.0 25.5 41.0 58-59 34.458787414334864 38.0 34.0 40.0 25.0 41.0 60-61 33.802618317241496 37.0 32.0 40.0 22.5 41.0 62-63 33.72295024011488 37.0 32.5 40.0 23.0 41.0 64-65 33.31864947179117 36.5 32.0 39.0 22.0 40.5 66-67 32.844949243204425 36.0 31.5 39.0 22.0 40.0 68-69 32.47842962448974 35.5 31.0 38.5 21.0 40.0 70-71 31.891091485198956 35.0 31.0 37.5 20.0 40.0 72-73 31.40287501070471 35.0 30.5 37.0 19.0 39.0 74-75 31.096419905710718 34.0 30.0 37.0 18.0 39.0 76-77 30.18481643892167 34.0 29.5 35.5 17.5 38.0 78-79 30.548237949789968 34.0 30.0 36.0 16.5 38.0 80-81 30.21826871348047 34.0 30.0 35.5 12.0 37.0 82-83 29.86170546346455 34.0 29.5 35.0 9.0 37.0 84-85 29.53986368237642 34.0 29.0 35.0 7.0 36.5 86-87 29.39253393118683 34.0 29.0 35.0 4.0 36.0 88-89 29.039122147880907 34.0 29.0 35.0 2.0 36.0 90-91 28.656436989330423 34.0 29.0 35.0 2.0 35.0 92-93 28.418046494673995 34.0 29.0 35.0 2.0 35.0 94-95 28.006556552709995 34.0 28.5 35.0 2.0 35.0 96-97 27.472728789851715 33.0 26.5 35.0 2.0 35.0 98-99 27.219169665815414 34.0 27.0 35.0 2.0 35.0 100-101 26.16498033627063 33.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.034161555169873736 1101 2 -0.052105016510459734 1101 3 -0.038603087990455975 1101 4 0.08241921108452743 1101 5 0.1330780997870349 1101 6 0.16904280897554713 1101 7 -0.003982278726383015 1101 8 -0.030979164554253202 1101 9 0.15089530353755976 1101 10-11 0.10099751331646445 1101 12-13 0.09225470624660659 1101 14-15 0.10603329986193444 1101 16-17 0.14249110623728 1101 18-19 -0.052843498387879606 1101 20-21 -0.09150081628152407 1101 22-23 -0.07249781785822762 1101 24-25 -0.05314835997685208 1101 26-27 0.005878616967805783 1101 28-29 -0.06669902633024805 1101 30-31 -0.02553329629574108 1101 32-33 0.15833601400621689 1101 34-35 -0.08419127012331273 1101 36-37 -0.07743029568725035 1101 38-39 -0.12542427603313655 1101 40-41 -0.2210777342440764 1101 42-43 -0.4158193797943994 1101 44-45 -0.2759940191985706 1101 46-47 -0.37624824542738367 1101 48-49 -0.5583351144378312 1101 50-51 -0.5252616003862585 1101 52-53 -0.44658537769468865 1101 54-55 -0.40062723840022585 1101 56-57 -0.4244652478003772 1101 58-59 -0.6852648392634535 1101 60-61 -0.6556960733996391 1101 62-63 -0.7194585971922507 1101 64-65 -0.782287665576554 1101 66-67 -0.6151748607916261 1101 68-69 -0.5675367435390868 1101 70-71 -0.6618808223092074 1101 72-73 -0.668686091310807 1101 74-75 -0.6726631031780883 1101 76-77 -0.6272113622148012 1101 78-79 -0.5802525924252784 1101 80-81 -0.47302944156283644 1101 82-83 -0.5690236139010842 1101 84-85 -0.6532872986543126 1101 86-87 -0.636286537879247 1101 88-89 -0.558666262866101 1101 90-91 -0.7105705813500194 1101 92-93 -0.6056595941658607 1101 94-95 -0.6887799957522347 1101 96-97 -0.4749601972678228 1101 98-99 -0.6966175977595768 1101 100-101 -0.5341976249850156 1102 1 -0.07156667106008285 1102 2 -0.024075716249324586 1102 3 -0.1111018110947164 1102 4 -0.004930169171423415 1102 5 -0.11281695017375881 1102 6 -0.039015122525292156 1102 7 -0.03685173322213586 1102 8 -0.012146211292233033 1102 9 -0.09597270500864852 1102 10-11 -0.08885044616817339 1102 12-13 -0.06844424130355264 1102 14-15 -0.004426724309169572 1102 16-17 0.030101890431105005 1102 18-19 -0.07721665048594417 1102 20-21 -0.03455971894250354 1102 22-23 -0.09467749687181737 1102 24-25 -0.07563674202405224 1102 26-27 0.07076407402454521 1102 28-29 0.022484048146942825 1102 30-31 -0.16209243994831013 1102 32-33 -0.11644457573977007 1102 34-35 -0.06493855986713726 1102 36-37 -0.15807444462724618 1102 38-39 -0.2413120063239731 1102 40-41 0.04801635318910513 1102 42-43 0.1516762499849733 1102 44-45 -2.5971422551407386E-4 1102 46-47 0.06623380609446627 1102 48-49 0.07160026744391956 1102 50-51 0.1995670078333589 1102 52-53 0.2684682339419666 1102 54-55 0.15929711901548416 1102 56-57 0.24145035838338913 1102 58-59 0.023640609304621307 1102 60-61 0.1286833552704394 1102 62-63 -0.07292995516188228 1102 64-65 0.04314045418548318 1102 66-67 0.005609045286398384 1102 68-69 -0.11803752046719751 1102 70-71 -0.05592338430159671 1102 72-73 0.061050202742091386 1102 74-75 0.045499120131193393 1102 76-77 0.10834685703718705 1102 78-79 0.14427540576189202 1102 80-81 0.08945527165716882 1102 82-83 -0.04181386489202765 1102 84-85 -0.20210814838346636 1102 86-87 -0.2051988924749928 1102 88-89 -0.15113329737465975 1102 90-91 -0.21727185608762767 1102 92-93 -0.0014160739621367213 1102 94-95 -0.16246758072874812 1102 96-97 0.056738829413905734 1102 98-99 -0.07583629942637415 1102 100-101 -0.24993158825358108 1103 1 -0.012936952153353332 1103 2 -0.05388501604326734 1103 3 -0.04457659823362903 1103 4 -0.045541731707871236 1103 5 0.085617986259507 1103 6 0.02103561422677558 1103 7 -0.025927196851078804 1103 8 -0.06006930915001618 1103 9 -0.17250491604243479 1103 10-11 -0.008675686244366432 1103 12-13 -0.07093714593401046 1103 14-15 -0.01983135476676523 1103 16-17 -0.026680796867211143 1103 18-19 -0.017853192611056556 1103 20-21 0.01526785385792806 1103 22-23 0.07613915100346702 1103 24-25 0.01931864462498112 1103 26-27 -0.15197697414621558 1103 28-29 -0.06928726712073541 1103 30-31 0.020316875397128342 1103 32-33 0.1769722820806905 1103 34-35 0.007577283218282105 1103 36-37 -0.06320711831800452 1103 38-39 -0.013817838134563942 1103 40-41 -0.01981304691197039 1103 42-43 0.26112145040485757 1103 44-45 0.2434458182187953 1103 46-47 0.24446375438574108 1103 48-49 0.28111593557501635 1103 50-51 0.2990258158112695 1103 52-53 0.25573049046388263 1103 54-55 0.35042324163902094 1103 56-57 0.2997147143571084 1103 58-59 0.19438523431919918 1103 60-61 0.3229355227600479 1103 62-63 0.1960245955943094 1103 64-65 0.16218335687199925 1103 66-67 0.10529840417601832 1103 68-69 -0.09068234376334061 1103 70-71 1.9117189762241082E-4 1103 72-73 0.10723299112512663 1103 74-75 0.27952919283443123 1103 76-77 0.2850486765620879 1103 78-79 0.280313176455099 1103 80-81 0.2729202419149388 1103 82-83 0.011817797951565012 1103 84-85 0.02481450236883731 1103 86-87 -0.07710599173240595 1103 88-89 0.04485636088123712 1103 90-91 0.11330617353797123 1103 92-93 -0.019140404275884038 1103 94-95 -0.15071463566440357 1103 96-97 -0.06370555455250226 1103 98-99 -0.17380090033613982 1103 100-101 -0.1561855247257249 1104 1 -0.03832005148894879 1104 2 -0.019131780372664053 1104 3 -0.061810277653339796 1104 4 0.014851831491832002 1104 5 -0.05401141008728416 1104 6 -0.030819029493891037 1104 7 0.00701406328261811 1104 8 -0.17322106565691797 1104 9 -0.08386745337709556 1104 10-11 -0.08917581831256172 1104 12-13 -0.08181265642835456 1104 14-15 -0.08565830480435466 1104 16-17 -0.05974579473380004 1104 18-19 -0.184066594484392 1104 20-21 -0.1387867410990964 1104 22-23 -0.02482494676763025 1104 24-25 -0.1345599948694982 1104 26-27 -0.14956175176370579 1104 28-29 -0.11972156827684444 1104 30-31 -0.0030769722571406533 1104 32-33 -0.029794688681441528 1104 34-35 0.015268020158764273 1104 36-37 0.18069153458647946 1104 38-39 -0.016436445526316845 1104 40-41 -0.02799558546572456 1104 42-43 0.0412288320006482 1104 44-45 0.11220407248053021 1104 46-47 -0.05343392324596863 1104 48-49 0.01781961519790798 1104 50-51 0.03867908602278192 1104 52-53 0.016776064147769887 1104 54-55 -0.04268858963879296 1104 56-57 -0.17861563897609045 1104 58-59 -0.07588611501131481 1104 60-61 0.08258218956216723 1104 62-63 -0.16155632156845456 1104 64-65 -0.15973650821526775 1104 66-67 -0.041885902059625835 1104 68-69 -0.14583767627040345 1104 70-71 0.06751506404870256 1104 72-73 0.03512406534744983 1104 74-75 -0.005712187176541761 1104 76-77 -0.14951956205464612 1104 78-79 -0.05828791666312938 1104 80-81 -0.08048010755780766 1104 82-83 -0.005956446719991959 1104 84-85 0.0852107069322976 1104 86-87 -0.11674889967072133 1104 88-89 -0.1348599972876876 1104 90-91 -0.1851190657086299 1104 92-93 -0.2768658853088617 1104 94-95 -0.24398464399485675 1104 96-97 -0.030324812126107048 1104 98-99 -0.01706400048446355 1104 100-101 0.01118073083637583 1105 1 0.014493190360909125 1105 2 0.023268911552172256 1105 3 0.060848699571842246 1105 4 0.08340712169256648 1105 5 -0.018109557074033944 1105 6 0.10842928284647968 1105 7 0.07833758810261315 1105 8 0.11947781589779538 1105 9 0.14251467897538106 1105 10-11 0.14808778221236452 1105 12-13 0.07271157387663862 1105 14-15 0.17744296993958386 1105 16-17 0.10640047118265983 1105 18-19 0.14229325728168618 1105 20-21 0.1106830077079266 1105 22-23 0.06050969916508109 1105 24-25 0.0752690445629085 1105 26-27 0.13578475930611233 1105 28-29 0.17153799631723388 1105 30-31 0.31148442804315124 1105 32-33 0.24184758903235348 1105 34-35 0.22285784950597787 1105 36-37 0.05757601721743555 1105 38-39 0.28186782119482956 1105 40-41 0.4225134110737585 1105 42-43 0.6271535008696532 1105 44-45 0.3888962819028805 1105 46-47 0.2026993713100751 1105 48-49 0.190646154030361 1105 50-51 0.33624947617168743 1105 52-53 0.35685364251788343 1105 54-55 0.36981349568136324 1105 56-57 0.47897869555476547 1105 58-59 0.36861526375329845 1105 60-61 0.33219545746179335 1105 62-63 0.2567107260910291 1105 64-65 0.0829766917943644 1105 66-67 0.12333590909556591 1105 68-69 0.15616931613145368 1105 70-71 0.15742425560465279 1105 72-73 0.09598184785145492 1105 74-75 -0.10052767983398425 1105 76-77 0.010620424260299188 1105 78-79 0.033903083496557684 1105 80-81 0.12097642723336222 1105 82-83 0.17201313838381793 1105 84-85 0.07709290128681445 1105 86-87 0.2854554956042783 1105 88-89 0.23633100376411775 1105 90-91 0.18997906700426626 1105 92-93 0.27498219900676446 1105 94-95 0.15769775215619575 1105 96-97 0.26538875097456227 1105 98-99 0.22430545044516847 1105 100-101 0.1699274556570849 1106 1 -0.06383228967436594 1106 2 0.0059541859385063844 1106 3 0.04721120146556501 1106 4 6.415824565095818E-4 1106 5 0.04969190468823825 1106 6 0.039903835053110015 1106 7 0.044982517384084986 1106 8 0.058408307411959015 1106 9 0.0963378690588641 1106 10-11 -0.08118077338937013 1106 12-13 0.0023733689735365715 1106 14-15 0.03267174175627474 1106 16-17 0.07100709572767272 1106 18-19 0.12018206351564231 1106 20-21 -0.029018618759955928 1106 22-23 0.09613881923313983 1106 24-25 0.13811962666515143 1106 26-27 0.16701897825580403 1106 28-29 0.11831834643318473 1106 30-31 0.12490470606154958 1106 32-33 0.24190913512855872 1106 34-35 0.061324960825992036 1106 36-37 0.1120933550076586 1106 38-39 0.06891893528842985 1106 40-41 -0.11826688660055851 1106 42-43 -0.013907966564090657 1106 44-45 0.17402951281314927 1106 46-47 0.0879001208371264 1106 48-49 -0.09906698046496842 1106 50-51 0.20528775954301892 1106 52-53 0.3123261951440526 1106 54-55 -0.05965892681182794 1106 56-57 0.05812321210107285 1106 58-59 0.17185482203927194 1106 60-61 0.2215316919186776 1106 62-63 0.017514797310667518 1106 64-65 -0.03497901152577043 1106 66-67 -0.16701138083680434 1106 68-69 0.056626335348404666 1106 70-71 0.10880514606376934 1106 72-73 0.13040250364392492 1106 74-75 0.1112558362034477 1106 76-77 0.026544960734433687 1106 78-79 0.2213442080396355 1106 80-81 0.19555184414443616 1106 82-83 0.2286519349063525 1106 84-85 0.2792097956538022 1106 86-87 0.3279821485843222 1106 88-89 0.2091965802920157 1106 90-91 -0.0019463036804658884 1106 92-93 0.06057902861803299 1106 94-95 0.26721544926880014 1106 96-97 0.2632657141372228 1106 98-99 0.08484879443516036 1106 100-101 0.2841851733190417 1107 1 -0.05704582777964973 1107 2 -0.004165465960358716 1107 3 -0.09240549086051786 1107 4 -0.19768650013465816 1107 5 -0.11410162368464682 1107 6 0.015361247409060752 1107 7 -0.09037572516245262 1107 8 -0.022345712447176425 1107 9 -0.08341055676724096 1107 10-11 -0.030009634153117304 1107 12-13 0.07314682741576917 1107 14-15 0.013220311470888646 1107 16-17 -0.0049047138276918645 1107 18-19 -0.05488395530313994 1107 20-21 -0.07194291158776167 1107 22-23 -0.11953886734999486 1107 24-25 -0.21821602999923329 1107 26-27 -0.13088438676376057 1107 28-29 -0.15943171229682207 1107 30-31 -0.24264457994937771 1107 32-33 -0.09499409984599794 1107 34-35 -0.11728871739231295 1107 36-37 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-0.3692271667533369 2316 84-85 -0.4510027149570881 2316 86-87 -0.5482554842348115 2316 88-89 -0.5556897733654509 2316 90-91 -0.7179246647728768 2316 92-93 -0.6149718231806922 2316 94-95 -0.9024792862637945 2316 96-97 -1.0877015238102636 2316 98-99 -1.1945598494193028 2316 100-101 -1.4942026292744082 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 12.0 5 36.0 6 115.0 7 296.0 8 783.0 9 1423.0 10 2521.0 11 4407.0 12 6927.0 13 9871.0 14 11067.0 15 13581.0 16 16958.0 17 21192.0 18 19364.0 19 14902.0 20 14205.0 21 14310.0 22 15223.0 23 16851.0 24 19559.0 25 23375.0 26 28603.0 27 35552.0 28 44378.0 29 55363.0 30 68224.0 31 83703.0 32 103427.0 33 129067.0 34 167851.0 35 223498.0 36 314942.0 37 398041.0 38 334065.0 39 63309.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.4699333981864 19.367735336220964 13.639487256548358 18.522844009044277 2 14.446150947489823 26.55215772597172 35.393039077943754 23.608652248594698 3 27.9162199687165 24.73277210495791 28.66284996221375 18.68815796411185 4 12.250799833994646 16.488855024187153 42.53772998658343 28.722615155234767 5 11.40013218944812 41.88214059072434 33.32566253922315 13.392064680604381 6 26.604014218671363 40.23379824207753 16.402152651332496 16.76003488791861 7 27.045521917757082 33.396368523101316 22.410107881521366 17.14800167762024 8 23.79265140852029 29.13595970198087 19.377172507229798 27.694216382269047 9 21.52790800317079 24.118557685762408 24.548195350532602 29.805338960534204 10-11 25.701940050377058 24.674175136587003 27.89376593222263 21.730118880813308 12-13 31.64815209252383 22.907596940758488 25.40727305465221 20.03697791206547 14-15 22.93712233369068 27.127129523047444 30.357684772397786 19.578063370864086 16-17 19.43417646193405 28.98168012349393 30.03478207405683 21.549361340515187 18-19 18.594114714969248 34.20303157395473 27.993684769887157 19.20916894118887 20-21 22.615197101131 26.955638519819917 26.498759196222686 23.930405182826398 22-23 19.77929632175175 28.495653338661903 27.495009958125372 24.23004038146098 24-25 24.70041084129142 28.230791358059932 27.153930440243563 19.91486736040509 26-27 23.84111501196058 23.960731790283482 33.06704490778836 19.131108289967585 28-29 19.40886433381779 33.15072951488215 28.651626173199336 18.788779978100717 30-31 22.039685202884087 27.089233509957978 27.632305970568805 23.23877531658913 32-33 22.27852726732116 28.507196021929982 28.875704197361042 20.33857251338782 34-35 25.24749300782842 25.64947699259322 29.379967428426866 19.723062571151495 36-37 23.604896460312077 24.99970898852097 32.25713993918409 19.138254611982862 38-39 21.106342944747418 29.008369021378567 31.360650600329983 18.524637433544033 40-41 20.777598207252204 29.91882870531115 28.504957881451386 20.798615205985254 42-43 19.263845307603965 30.905190302301015 25.76710502912779 24.063859360967225 44-45 22.65762274185509 25.968529249660193 26.539908257888 24.83393975059672 46-47 25.1800313082652 29.746211574747772 26.698212082425627 18.375545034561398 48-49 24.174661346883116 28.36928286126476 27.69107633391669 19.764979457935432 50-51 18.601536647438447 29.46983687119434 32.383999367598655 19.544627113768563 52-53 22.07726275617195 31.77616944842745 25.961897818874107 20.184669976526493 54-55 19.765791917998627 28.671474088013575 27.313941155933048 24.24879283805475 56-57 23.637030778027714 26.212662724076356 28.06664781156374 22.08365868633219 58-59 28.047444237693735 25.696378310176716 25.77239835557794 20.483779096551604 60-61 22.378977489214567 28.4592942499278 31.231521468202594 17.930206792655035 62-63 20.3736520570563 28.189563367621084 30.368212078772288 21.068572496550324 64-65 19.43429348085164 33.971542446332066 26.80561752311509 19.788546549701206 66-67 22.09788286808207 27.414433536747147 25.7253316090308 24.76235198613998 68-69 20.145187095436835 29.40654626518395 26.420963964316098 24.02730267506312 70-71 24.68174510008006 28.747089955455074 25.70977419240088 20.861390752063986 72-73 23.627063264288868 24.86349573019299 31.192120454889803 20.31732055062834 74-75 19.606993218591988 33.39205005457757 27.292625561887622 19.708331164942823 76-77 22.057675881134898 27.439038047285173 26.619156051619758 23.884130019960168 78-79 22.11986201353954 29.11793187192994 27.296462285384283 21.465743829146238 80-81 24.779021842000674 27.101493828597278 27.08592533711603 21.03355899228602 82-83 22.701363834987166 26.034053055837962 30.64039419683931 20.62418891233556 84-85 20.590289694776253 29.442263610849988 29.51272806802005 20.454718626353706 86-87 20.846797604230805 30.04952977891221 26.34252578157183 22.76114683528516 88-89 19.785466626556026 30.58088698680521 24.559394124376656 25.07425226226211 90-91 22.412940512552506 25.304683502888626 26.19845544754472 26.083920537014144 92-93 23.85865829905996 28.986137674651463 26.622340016732288 20.532864009556288 94-95 22.813886673529403 27.99793766622516 27.233719157218694 21.954456503026748 96-97 17.961266953064133 29.47112500450149 31.64438024753384 20.92322779490053 98-99 21.087265897782785 31.234438291919254 25.841850117049738 21.836445693248223 100-101 18.134469270688427 29.73526391153988 26.367987576655022 25.76227924111667 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 471.0 1 338.5 2 547.5 3 846.0 4 635.0 5 496.5 6 640.0 7 1023.5 8 1486.0 9 1732.5 10 1747.5 11 1750.5 12 1837.0 13 2026.0 14 2277.5 15 2502.5 16 2769.0 17 2982.0 18 3273.5 19 3670.0 20 4062.0 21 4433.0 22 5086.0 23 6181.0 24 7488.0 25 9138.0 26 11269.5 27 13967.0 28 17922.5 29 22821.0 30 27583.5 31 31960.5 32 36047.0 33 40029.5 34 43424.5 35 46781.0 36 50496.5 37 53826.5 38 57039.5 39 59059.0 40 60074.0 41 61430.5 42 62568.5 43 64420.5 44 65703.5 45 67928.5 46 77748.0 47 143402.5 48 294374.5 49 290885.5 50 145011.0 51 78078.0 52 53650.5 53 43333.0 54 37275.5 55 32162.0 56 27137.0 57 21932.5 58 17459.5 59 14029.5 60 11142.0 61 8483.0 62 6160.0 63 4241.0 64 2829.5 65 1747.0 66 1022.5 67 592.5 68 325.0 69 193.5 70 108.5 71 53.0 72 32.5 73 20.5 74 14.5 75 12.0 76 7.0 77 3.0 78 3.5 79 3.0 80 2.0 81 1.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04923068815367353 2 0.005445678261423299 3 0.04721051718572618 4 0.0 5 0.0 6 0.0012735860450102875 7 0.0 8 0.0 9 0.0 10-11 2.8545894112299544E-4 12-13 0.0 14-15 0.002547172090020575 16-17 0.0 18-19 0.0 20-21 0.012516276649239032 22-23 0.0 24-25 0.0 26-27 0.007926975211184722 28-29 0.010891356522846597 30-31 0.011725774966129199 32-33 0.006675347546260817 34-35 0.008915102315072013 36-37 0.021629004385088503 38-39 0.010781564622414676 40-41 0.013460486992953555 42-43 0.0 44-45 0.0 46-47 2.1958380086384267E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 9.222519636281393E-4 58-59 1.7566704069107414E-4 60-61 0.010430230541032526 62-63 0.01631507640418351 64-65 0.04297254982905401 66-67 0.024549468936577612 68-69 0.0018445039272562786 70-71 3.513340813821483E-4 72-73 0.0 74-75 2.1958380086384267E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.1958380086384267E-5 86-87 9.222519636281393E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.317502805183056E-4 96-97 5.709178822459909E-4 98-99 5.050427419868381E-4 100-101 0.001383377945442209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2277035.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.38846081549796 #Duplication Level Percentage of deduplicated Percentage of total 1 81.56316961199033 38.65153067145617 2 11.870835422929057 11.250812385733973 3 2.8917482978858797 4.111065027079437 4 1.1490352382293207 2.1780404544982606 5 0.6119649472105754 1.4500038460673315 6 0.38915755477104347 1.1064946521193557 7 0.25424546750153293 0.8433810961950048 8 0.18732474943647515 0.7101625238754701 9 0.14332270437272504 0.6112658124124271 >10 0.8290240028570341 7.319260933917514 >50 0.0679307543021221 2.2337678610666676 >100 0.03343781534888785 3.0996200803370697 >500 0.003090566962261357 0.9999477060002837 >1k 0.003277874050883257 3.4577659989431497 >5k 0.0011238425317314023 3.4189326474385453 >10k+ 0.0013111496203533027 18.55794830285938 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 122922 5.398335993957054 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 43918 1.9287362732676485 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 34893 1.5323875127084126 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 28907 1.26950178631422 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 24709 1.0851392271089377 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 24316 1.0678799403610395 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 23325 1.0243584310298262 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 22996 1.0099098169329852 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 19491 0.8559815725274315 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 18737 0.822868335357164 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 16699 0.7333659781250618 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 13609 0.597663189191207 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 13083 0.5745629733403307 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 10546 0.46314615278201693 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 9286 0.4078110349643286 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 8027 0.352519833906813 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 6561 0.2881378634935343 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6497 0.28532719084247715 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6362 0.27939842821915345 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6337 0.27830050921483424 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6083 0.267145652130951 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5958 0.2616560571093549 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5593 0.2456264396462944 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5562 0.2442650200809386 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 5391 0.2367552540913952 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5379 0.23622825296932196 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4925 0.21629004385088504 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4914 0.21580695948898457 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4714 0.20702360745443088 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3478 0.15274249188088895 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3305 0.145144892371 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3195 0.14031404875199546 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 3141 0.13794254370266598 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3121 0.1370642084992106 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2934 0.12885177434690287 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2927 0.1285443570256935 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2701 0.11861916922664781 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2434 0.10689339426051862 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.3916760172768534E-5 0.0 12-13 0.0 0.0 0.0 8.783352034553707E-5 0.0 14-15 0.0 0.0 0.0 1.0979190043192133E-4 0.0 16-17 0.0 0.0 0.0 3.074173212093798E-4 0.0 18-19 0.0 0.0 0.0 6.148346424187595E-4 0.0 20-21 0.0 0.0 0.0 0.0010540022441464449 0.0 22-23 0.0 4.3916760172768534E-5 0.0 0.0022397547688111953 0.0 24-25 0.0 4.3916760172768534E-5 0.0 0.004369717637190469 0.0 26-27 0.0 4.3916760172768534E-5 0.0 0.007487807609457036 0.0 28-29 0.0 4.3916760172768534E-5 0.0 0.017259286747898034 0.0 30-31 0.0 4.3916760172768534E-5 0.0 0.04286275792862209 0.0 32-33 0.0 4.3916760172768534E-5 0.0 0.09630945505888139 0.0 34-35 0.0 4.3916760172768534E-5 0.0 0.13596628949489137 0.0 36-37 0.0 4.3916760172768534E-5 0.0 0.15217157399864298 0.0 38-39 0.0 4.3916760172768534E-5 0.0 0.1677839822400622 0.0 40-41 0.0 4.3916760172768534E-5 0.0 0.19411207996363694 0.0 42-43 0.0 4.3916760172768534E-5 0.0 0.2224823070352454 0.0 44-45 0.0 8.783352034553707E-5 0.0 0.24865669609821545 0.0 46-47 0.0 1.317502805183056E-4 0.0 0.27902513575768484 0.0 48-49 0.0 1.317502805183056E-4 0.0 0.3122701232084707 0.0 50-51 0.0 1.317502805183056E-4 0.0 0.35489133895614255 0.0 52-53 0.0 1.317502805183056E-4 0.0 0.45996218766949126 0.0 54-55 0.0 1.317502805183056E-4 0.0 0.5761000599463777 0.0 56-57 0.0 1.317502805183056E-4 0.0 0.7310164314558186 0.0 58-59 0.0 1.317502805183056E-4 0.0 0.9746007417540793 0.0 60-61 0.0 1.317502805183056E-4 0.0 1.260301225058025 0.0 62-63 0.0 1.317502805183056E-4 0.0 1.3919417136758987 0.0 64-65 0.0 1.317502805183056E-4 0.0 1.5399192370780423 0.0 66-67 0.0 1.317502805183056E-4 0.0 2.0804467212844773 0.0 68-69 0.0 1.317502805183056E-4 0.0 2.6063499243533808 0.0 70-71 0.0 1.317502805183056E-4 0.0 2.9137452871826737 0.0 72-73 0.0 1.7566704069107414E-4 0.0 3.089412327873748 0.0 74-75 0.0 1.7566704069107414E-4 0.0 3.780662132993125 0.0 76-77 0.0 1.7566704069107414E-4 0.0 3.9628727709499416 0.0 78-79 0.0 2.1958380086384266E-4 0.0 4.095369636391184 0.0 80-81 0.0 2.1958380086384266E-4 0.0 4.325954585678305 0.0 82-83 0.0 2.1958380086384266E-4 0.0 4.853021582891786 0.0 84-85 0.0 2.1958380086384266E-4 0.0 5.315333317230522 0.0 86-87 0.0 2.1958380086384266E-4 0.0 6.270347183947546 0.0 88-89 0.0 2.1958380086384266E-4 0.0 7.552343288530919 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 475 0.0 57.021843 2 ATAACGC 525 0.0 50.68052 3 TACCTGG 2290 0.0 50.215744 2 TAACGCA 570 0.0 47.49003 4 GTACCCG 180 0.0 44.896057 1 ACCTGGG 2860 0.0 39.04039 3 GTATAAC 825 0.0 37.453396 1 GTACCTG 3190 0.0 37.105686 1 TACGCAG 1685 0.0 34.94815 4 AGTACGC 1720 0.0 33.428505 2 GTACGCA 1760 0.0 32.93514 3 GAGTACG 1835 0.0 32.90027 1 CCTGGGG 1720 0.0 31.199846 4 GTACACG 230 0.0 31.002394 1 CGCAGTG 1865 0.0 30.811228 6 TACCCGG 280 0.0 30.547415 2 ACGCAGT 1905 0.0 30.16427 5 GTACCGG 160 1.891749E-9 29.710629 1 TATCACG 335 0.0 26.950623 2 GTAGGCA 1160 0.0 23.75649 3 >>END_MODULE