##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003859_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1806841 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.661177159473358 31.0 31.0 34.0 30.0 34.0 2 31.864106470906957 31.0 31.0 34.0 30.0 34.0 3 31.9030246712356 31.0 31.0 34.0 30.0 34.0 4 35.652457521165395 37.0 35.0 37.0 33.0 37.0 5 35.53438072304093 37.0 35.0 37.0 33.0 37.0 6 35.58012575539298 37.0 35.0 37.0 33.0 37.0 7 35.510711789249854 37.0 35.0 37.0 33.0 37.0 8 35.511248637815946 37.0 35.0 37.0 33.0 37.0 9 37.07563034046715 39.0 37.0 39.0 33.0 39.0 10-11 36.99435976934329 39.0 37.0 39.0 33.0 39.0 12-13 36.94804025368032 39.0 37.0 39.0 33.0 39.0 14-15 38.01492383668513 40.0 37.0 41.0 33.0 41.0 16-17 38.046670957765514 40.0 37.0 41.0 33.0 41.0 18-19 38.080428770434146 40.0 37.0 41.0 33.0 41.0 20-21 38.012135544854246 40.0 37.0 41.0 33.0 41.0 22-23 37.91314509688456 40.0 37.0 41.0 33.0 41.0 24-25 37.80134278555778 39.5 37.0 41.0 32.5 41.0 26-27 37.557408482539415 39.0 36.5 41.0 32.0 41.0 28-29 37.38481083836375 39.0 36.0 41.0 32.0 41.0 30-31 37.15152412414817 39.0 36.0 40.0 31.0 41.0 32-33 36.867029528331486 39.0 35.0 40.0 31.0 41.0 34-35 36.79642148921792 39.0 35.0 40.0 30.5 41.0 36-37 36.58982887813593 39.0 35.0 40.0 30.0 41.0 38-39 36.335905317623414 38.0 35.0 40.0 30.0 41.0 40-41 36.4679360275752 39.0 35.0 40.0 30.0 41.0 42-43 36.30521916427621 39.0 35.0 40.5 30.0 41.0 44-45 36.11704627025842 39.0 35.0 40.5 30.0 41.0 46-47 35.793168574323914 39.0 35.0 40.0 28.5 41.0 48-49 35.489570471336435 39.0 35.0 40.0 27.0 41.0 50-51 35.1879664563733 38.0 34.0 40.0 26.5 41.0 52-53 35.02075196433997 38.0 34.0 40.0 26.0 41.0 54-55 34.814151604928156 38.0 34.0 40.0 26.0 41.0 56-57 34.525860880951896 38.0 34.0 40.0 24.5 41.0 58-59 34.24743876190544 38.0 33.5 40.0 24.0 41.0 60-61 33.76505984754608 37.0 33.0 40.0 22.5 41.0 62-63 33.56420155398289 37.0 33.0 40.0 22.0 41.0 64-65 33.124120772110004 36.5 32.0 39.5 21.5 41.0 66-67 32.75881331008097 36.0 32.0 39.0 20.5 40.5 68-69 32.446080202961966 35.5 31.5 39.0 20.0 40.0 70-71 31.98055528959106 35.0 31.0 38.0 18.5 40.0 72-73 31.58432811741598 35.0 31.0 37.5 17.5 40.0 74-75 31.21860944045436 35.0 31.0 37.0 15.0 39.0 76-77 30.26517330523272 34.0 29.5 36.0 13.0 39.0 78-79 30.627588979882567 34.0 30.0 36.0 12.0 39.0 80-81 30.403097450190693 34.0 30.0 36.0 8.0 37.5 82-83 30.0836180383332 34.0 30.0 35.0 7.0 37.0 84-85 29.75267082161629 34.0 30.0 35.0 4.0 37.0 86-87 29.44080303690253 34.0 29.5 35.0 2.0 36.0 88-89 29.11586907757794 34.0 29.0 35.0 2.0 36.0 90-91 28.812041291956515 34.0 29.0 35.0 2.0 36.0 92-93 28.555543902313484 34.0 29.0 35.0 2.0 35.0 94-95 28.228231759186336 34.0 29.0 35.0 2.0 35.0 96-97 27.81958180050154 34.0 29.0 35.0 2.0 35.0 98-99 27.47516272876252 34.0 27.5 35.0 2.0 35.0 100-101 26.48733314110096 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06025770006158737 1101 2 0.1123426427618206 1101 3 0.00872085006018608 1101 4 0.01222061477287184 1101 5 0.17521682411257444 1101 6 0.08240721189169875 1101 7 -0.0024401948275851737 1101 8 -0.022655153005032957 1101 9 0.15660356527259722 1101 10-11 0.1955960847500151 1101 12-13 0.13091574559150843 1101 14-15 0.09767977856695609 1101 16-17 0.16703882238775236 1101 18-19 -0.04216020022342093 1101 20-21 -0.012254376691686275 1101 22-23 0.012555975643778083 1101 24-25 -0.0295452925102353 1101 26-27 0.04899679198900486 1101 28-29 0.12843045684968502 1101 30-31 0.07490157634723005 1101 32-33 0.24110503993831145 1101 34-35 0.24710515724214588 1101 36-37 0.15239090340121209 1101 38-39 0.11388580467602338 1101 40-41 0.28462560356491196 1101 42-43 0.04609316082940751 1101 44-45 0.012261379925313065 1101 46-47 0.07436016703842796 1101 48-49 0.23848809183038355 1101 50-51 -0.059600906719389 1101 52-53 0.16749039869834093 1101 54-55 0.112026969256938 1101 56-57 0.1816276269739987 1101 58-59 -0.0762819300351083 1101 60-61 0.013792503826280722 1101 62-63 -0.19430733733705097 1101 64-65 -0.4210064258742605 1101 66-67 -0.4187856320963661 1101 68-69 -0.5137584026642728 1101 70-71 -0.2357749415876036 1101 72-73 -0.2934296322488912 1101 74-75 -0.46232298326226484 1101 76-77 -0.36761530027799694 1101 78-79 -0.2475802980379882 1101 80-81 -0.15979120070817388 1101 82-83 -0.12203571165091986 1101 84-85 -0.06714879311044442 1101 86-87 0.006888991529510946 1101 88-89 -0.02681443655418292 1101 90-91 -0.05375990670124153 1101 92-93 0.22833231720493785 1101 94-95 0.17519839377496282 1101 96-97 0.059262287611073106 1101 98-99 -0.10261715917759773 1101 100-101 0.1116851829092873 1102 1 0.0016447277248516912 1102 2 0.06023345348848963 1102 3 0.061858792253939754 1102 4 0.07948253985442477 1102 5 -0.046284849283637186 1102 6 -0.05197053187044531 1102 7 -0.03206238006045936 1102 8 0.06484182586962106 1102 9 0.1394517661839174 1102 10-11 -0.08661730433133386 1102 12-13 0.004456286021337519 1102 14-15 -0.006949746122977274 1102 16-17 0.0079908080010469 1102 18-19 -0.1677787969738489 1102 20-21 0.03156511772554893 1102 22-23 0.011864632925956187 1102 24-25 -0.036321898554120935 1102 26-27 -0.13634341062097377 1102 28-29 -0.07441902761318886 1102 30-31 -0.12937551785589108 1102 32-33 -0.07672908969766468 1102 34-35 -0.2901471958219304 1102 36-37 -0.24910552655033058 1102 38-39 -0.24096049002468334 1102 40-41 -0.2178102768900132 1102 42-43 -0.07397270994425043 1102 44-45 -0.019841610067082627 1102 46-47 -0.03283250087810785 1102 48-49 0.05785825034899972 1102 50-51 0.049592326506022744 1102 52-53 0.151781093086079 1102 54-55 0.02817404787993638 1102 56-57 0.08268136818542615 1102 58-59 -0.15809796432505152 1102 60-61 -0.09626603431772907 1102 62-63 -0.3512048566688364 1102 64-65 -0.2708999866784154 1102 66-67 -0.07436846691556553 1102 68-69 -0.1656091065011367 1102 70-71 -0.015522136549527943 1102 72-73 -0.1164535431758722 1102 74-75 -0.18193537800079085 1102 76-77 -0.09010858552821688 1102 78-79 -0.10599841632409124 1102 80-81 -0.09442674716659027 1102 82-83 -0.11379449134841124 1102 84-85 -0.17349663831412343 1102 86-87 -0.3667276061576388 1102 88-89 -0.21210520877430383 1102 90-91 -0.04353477995052302 1102 92-93 0.14257755475073353 1102 94-95 0.014120456251152547 1102 96-97 0.14048188006979956 1102 98-99 -0.03214102982290967 1102 100-101 -0.1346797034895708 1103 1 0.04595986169669075 1103 2 -0.03561814250924655 1103 3 -0.019902685382845675 1103 4 -0.002071192324208937 1103 5 -4.027809542677119E-4 1103 6 -0.03992729083744706 1103 7 -0.06814683683306555 1103 8 -0.08525741530487352 1103 9 0.030860247414729258 1103 10-11 -0.079974695767838 1103 12-13 -0.09285132684544806 1103 14-15 -0.026421311225696797 1103 16-17 -0.07546036267308409 1103 18-19 -0.003933881206684475 1103 20-21 0.017770279705544567 1103 22-23 -0.054776093038000795 1103 24-25 -0.1790605652599595 1103 26-27 -0.12250690359854843 1103 28-29 -0.141567575808196 1103 30-31 -0.040224986935847085 1103 32-33 0.07453118778191481 1103 34-35 -0.1174174894505029 1103 36-37 -0.10069717752116958 1103 38-39 -0.09445917235489532 1103 40-41 -0.12459132247020221 1103 42-43 -0.08246374346442309 1103 44-45 0.07083748375502807 1103 46-47 -0.07168210066082992 1103 48-49 -0.020832011964863284 1103 50-51 0.02477408879305898 1103 52-53 0.054949526088769574 1103 54-55 -0.1165842164472366 1103 56-57 0.04912612992716703 1103 58-59 0.15158396328899215 1103 60-61 0.08490014616061359 1103 62-63 -0.04778931748843007 1103 64-65 -0.09989339847938083 1103 66-67 -0.27416803601611406 1103 68-69 -0.12857090921609426 1103 70-71 -0.01155293923541123 1103 72-73 0.031503341697465714 1103 74-75 0.14730220216332413 1103 76-77 0.08661699830996739 1103 78-79 0.1006744397382846 1103 80-81 0.01762629657416781 1103 82-83 -0.07286608798766991 1103 84-85 -0.14035359342521758 1103 86-87 -0.13633279685627642 1103 88-89 -0.15250864265983566 1103 90-91 -0.02662121195375633 1103 92-93 0.06757275082437175 1103 94-95 -0.08688115864752177 1103 96-97 -0.08823207961559376 1103 98-99 -0.2215876504818013 1103 100-101 -0.1323186598699202 1104 1 0.04854917146291271 1104 2 -0.09178195253998211 1104 3 -0.18911041111983096 1104 4 0.023850790844306857 1104 5 -0.0746375584547252 1104 6 -0.09951257286060411 1104 7 -0.007540500845941267 1104 8 -0.10433840182604825 1104 9 -0.12039627210895532 1104 10-11 -0.05481120055667077 1104 12-13 -0.05955850895071535 1104 14-15 -0.14240197416429368 1104 16-17 -0.0267107156280062 1104 18-19 0.013237254058616088 1104 20-21 0.0016687195636535535 1104 22-23 -0.04764315804337116 1104 24-25 -0.14777334271499143 1104 26-27 -0.03405688688390285 1104 28-29 -0.013088251664129302 1104 30-31 0.04287017620235645 1104 32-33 0.00562465024536607 1104 34-35 -0.05261691303859095 1104 36-37 0.040688288200819045 1104 38-39 -0.06541138793814127 1104 40-41 0.04269665304527592 1104 42-43 0.1587552249010855 1104 44-45 0.10436625404384614 1104 46-47 -0.04098362315420445 1104 48-49 0.010616028202214522 1104 50-51 0.14329813678843806 1104 52-53 -0.050275667265616164 1104 54-55 -0.10183329714165268 1104 56-57 -0.20246533164699798 1104 58-59 -0.10401576797843148 1104 60-61 -0.0980601638555143 1104 62-63 -0.32685024643333094 1104 64-65 -0.3778962740477567 1104 66-67 -0.33685623807185294 1104 68-69 -0.3419882268885104 1104 70-71 -0.2916255100130165 1104 72-73 -0.1979400767886048 1104 74-75 -0.34238891765310697 1104 76-77 -0.15283476127174467 1104 78-79 -0.136043257989499 1104 80-81 -0.2682769272430683 1104 82-83 -0.44892097694074806 1104 84-85 -0.42536915589742463 1104 86-87 -0.38399550501854307 1104 88-89 -0.38932792649401193 1104 90-91 -0.4550948563634378 1104 92-93 -0.5375333303860934 1104 94-95 -0.4032576179335656 1104 96-97 -0.328923481795961 1104 98-99 -0.516504775043245 1104 100-101 -0.3206729324906803 1105 1 0.004418613855019515 1105 2 0.06408353882900641 1105 3 0.14485495421152095 1105 4 0.1256105765408151 1105 5 0.07662775669863464 1105 6 0.17800729090811984 1105 7 0.1097306065073056 1105 8 0.11561624042856522 1105 9 0.048092343017934525 1105 10-11 0.20857348290711286 1105 12-13 0.14311574104631575 1105 14-15 0.15965749663566697 1105 16-17 0.09432413634691272 1105 18-19 0.0325480770962443 1105 20-21 0.04717383811410514 1105 22-23 0.06124407580583835 1105 24-25 -0.00675342560745662 1105 26-27 0.13112037576826197 1105 28-29 0.16218130688805843 1105 30-31 0.07512988485390792 1105 32-33 0.1855206593169143 1105 34-35 0.13798557847658088 1105 36-37 -0.0871968476551146 1105 38-39 -0.05649195325052858 1105 40-41 0.08888095246960859 1105 42-43 0.21403556974637894 1105 44-45 0.11665413349023623 1105 46-47 0.034625920937244814 1105 48-49 0.016397757881115638 1105 50-51 0.1257125279950415 1105 52-53 0.18489219195152629 1105 54-55 0.2850692587752022 1105 56-57 0.17003633766027093 1105 58-59 0.03550987218608981 1105 60-61 0.06414257104268728 1105 62-63 -0.01024330606612267 1105 64-65 -0.08922504263238551 1105 66-67 0.04024382949064176 1105 68-69 -0.08552751388687341 1105 70-71 -0.0801351445828864 1105 72-73 -0.11786074024441007 1105 74-75 -0.20634491905967067 1105 76-77 -0.3398501188985996 1105 78-79 -0.25469090179686305 1105 80-81 -0.14778455074217334 1105 82-83 -0.004048812293714121 1105 84-85 -0.09082417547474719 1105 86-87 0.023496425016737987 1105 88-89 0.12096567403384029 1105 90-91 0.24556338408792655 1105 92-93 0.13932888558473522 1105 94-95 -0.02691634436077095 1105 96-97 0.1653769698059797 1105 98-99 0.09151185086203029 1105 100-101 0.019649233934316612 1106 1 -0.025785100559218677 1106 2 0.010865314558149208 1106 3 0.010906057104730138 1106 4 0.06117355157746829 1106 5 0.043356447111534635 1106 6 0.08683773921225679 1106 7 0.11384101851245276 1106 8 0.0836603795253339 1106 9 0.04252140323647069 1106 10-11 -0.005203967712439805 1106 12-13 -0.013736909227390015 1106 14-15 -0.04577425484871611 1106 16-17 0.02020478591177266 1106 18-19 0.1310402282199732 1106 20-21 0.058199605718819214 1106 22-23 0.09435204970328215 1106 24-25 -0.07892175435473092 1106 26-27 -0.062056573987632646 1106 28-29 0.002663193422577592 1106 30-31 0.048694575523860806 1106 32-33 0.16504941516145522 1106 34-35 0.19248955403318746 1106 36-37 0.13799779201320916 1106 38-39 -0.07381429952024376 1106 40-41 -0.30850017517467876 1106 42-43 -0.12612159277920654 1106 44-45 -0.06387148124590425 1106 46-47 -0.3065662689391573 1106 48-49 -0.3326340582559624 1106 50-51 0.032239723995921565 1106 52-53 0.0380660648622424 1106 54-55 -0.1201636661945713 1106 56-57 0.03659935553907445 1106 58-59 -0.247013890600833 1106 60-61 -0.3644468707046471 1106 62-63 -0.3206697217556993 1106 64-65 -0.3915496673254282 1106 66-67 -0.30123876199194655 1106 68-69 -0.2578990020478358 1106 70-71 -0.25061963085684624 1106 72-73 -0.30186898346318003 1106 74-75 -0.13698981023087953 1106 76-77 -0.3067455528992973 1106 78-79 -0.17705654784671765 1106 80-81 -0.19354824124270564 1106 82-83 -0.18443470320592326 1106 84-85 0.024888571816948257 1106 86-87 0.12963565615596906 1106 88-89 0.08116677897179159 1106 90-91 0.043384714803995195 1106 92-93 0.16928163264274687 1106 94-95 0.0058596028888580065 1106 96-97 0.04595959380965553 1106 98-99 -0.12087955889779778 1106 100-101 -0.12571344432309672 1107 1 -0.059307919459396174 1107 2 -0.18770480854027483 1107 3 -0.1961304423846002 1107 4 -0.22628008493276752 1107 5 -0.3713011349988804 1107 6 -0.1652049420757109 1107 7 -0.21511130100590492 1107 8 -0.16191829058163876 1107 9 -0.10030502260882201 1107 10-11 -0.06706898948312556 1107 12-13 -0.20399856057394317 1107 14-15 -0.01339708449985011 1107 16-17 -0.17222049495690328 1107 18-19 -0.17491848811609856 1107 20-21 -0.14030332391618572 1107 22-23 -0.22061731007740093 1107 24-25 -0.11879435554808992 1107 26-27 -0.21663177691189617 1107 28-29 -0.3153399512315076 1107 30-31 -0.21008089079877124 1107 32-33 -0.2729612551605314 1107 34-35 -0.34707059755526615 1107 36-37 -0.4332474372508841 1107 38-39 -0.36386496339166285 1107 40-41 -0.40544018485053357 1107 42-43 -0.18043070958832175 1107 44-45 -0.35221207147328926 1107 46-47 -0.5729513949940923 1107 48-49 -0.7935274625455904 1107 50-51 -0.669559045019831 1107 52-53 -0.777538273147762 1107 54-55 -0.6845686008825211 1107 56-57 -0.8986463037631864 1107 58-59 -0.8482072465111656 1107 60-61 -0.597117676488665 1107 62-63 -0.755115778843269 1107 64-65 -0.6871034758509325 1107 66-67 -0.8413364612877601 1107 68-69 -0.6398837625703351 1107 70-71 -0.5647423529984366 1107 72-73 -0.6311707089913199 1107 74-75 -0.682459626927109 1107 76-77 -0.6848566102556397 1107 78-79 -0.888635325463234 1107 80-81 -0.8565998995773825 1107 82-83 -0.8536983972337637 1107 84-85 -0.9653112615432597 1107 86-87 -0.6236131478003806 1107 88-89 -0.8068889109706916 1107 90-91 -0.9152409100952177 1107 92-93 -0.9559468251328838 1107 94-95 -0.8041878356963181 1107 96-97 -0.6315655365456649 1107 98-99 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-0.4940625310370095 2316 64-65 -0.523873192941636 2316 66-67 -0.4795308209460245 2316 68-69 -0.37926311992763573 2316 70-71 -0.5854866608458273 2316 72-73 -0.4229305100125096 2316 74-75 -0.31937456317312396 2316 76-77 -0.37986860032548364 2316 78-79 -0.4082224976537816 2316 80-81 -0.41488165682698863 2316 82-83 -0.2853892397005282 2316 84-85 -0.19960618752101666 2316 86-87 -0.22434284372845426 2316 88-89 -0.25517899552729517 2316 90-91 -0.48505195692536063 2316 92-93 -0.5054121243680889 2316 94-95 -0.7235696818540163 2316 96-97 -0.5492719426239709 2316 98-99 -0.6122208734098429 2316 100-101 -0.8996064599408555 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 23.0 5 24.0 6 105.0 7 290.0 8 756.0 9 1290.0 10 2354.0 11 3958.0 12 6214.0 13 8777.0 14 9868.0 15 12239.0 16 15082.0 17 19444.0 18 17417.0 19 12317.0 20 10750.0 21 10841.0 22 11414.0 23 12650.0 24 14372.0 25 17422.0 26 21385.0 27 26311.0 28 33257.0 29 41610.0 30 51043.0 31 63649.0 32 78276.0 33 98250.0 34 127628.0 35 169933.0 36 238759.0 37 320134.0 38 289796.0 39 59162.0 40 35.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.47995678664041 20.191392434782657 14.133935943005321 20.194714835571617 2 15.341830835538516 23.15407917189481 38.990838128837396 22.513251863729273 3 21.98589768010525 26.845129195589674 29.55966519449108 21.609307929813994 4 12.868370819568517 17.470878732550347 39.5170908785001 30.143659569381036 5 12.00559429412992 39.09209498788216 34.273685398991944 14.628625318995972 6 27.832062868499836 39.18798728594287 18.048404405753978 14.931545439803322 7 26.913823629195928 33.03749472145031 22.757840894688574 17.290840754665187 8 24.844964222087057 33.256274348434644 20.37794139052634 21.52082003895196 9 24.201908192253775 19.93850039931571 23.08587197213258 32.77371943629794 10-11 24.56755898155671 26.46319311769303 28.249022047380063 20.7202258533702 12-13 27.38268060111543 24.32067901934924 27.738356612452343 20.55828376708299 14-15 22.435992873553168 26.84458246692617 28.57535819029429 22.14406646922638 16-17 20.26022212247785 29.34073889180066 29.018131645230543 21.380907340490946 18-19 19.840484027094803 30.17257744317292 30.415100166533747 19.57183836319853 20-21 21.82229693414803 27.32778418315074 29.671495696714846 21.178423185986382 22-23 20.539853811154384 27.891469144213577 29.989772204637816 21.578904839994223 24-25 22.19362412077211 27.873814021266952 29.935644586324972 19.996917271635965 26-27 21.872958125115716 26.279298180359977 32.007216458040844 19.840527236483464 28-29 20.416746002577128 29.491122865126002 30.67880912001346 19.413322012283412 30-31 21.525909689703653 27.587011391185946 30.03448352207942 20.852595397030985 32-33 21.292968965125027 28.19241218436337 30.39008969541859 20.12452915509301 34-35 22.411314779052844 26.826300341119357 30.767936440989896 19.994448438837903 36-37 22.233176555966697 26.818174143829786 31.335582077836598 19.61306722236692 38-39 21.26596999220372 28.27130395506995 31.13328929186129 19.329436760865047 40-41 20.88720102755109 28.553787157327292 30.117780359017406 20.441231456104212 42-43 20.369335209905024 29.053275855484795 29.000504194890418 21.576884739719766 44-45 21.66809365074182 27.486342185062217 29.072093227904393 21.773470936291574 46-47 22.532847402094262 29.024595426269066 28.73519514732904 19.70736202430763 48-49 22.384177689127046 28.484410083676426 29.04190241421354 20.089509812982993 50-51 20.424569732477842 28.945906142267084 30.42376169236806 20.205762432887013 52-53 21.571792980123874 29.93016541023809 28.082686855124493 20.41535475451354 54-55 20.6892305410382 29.148441949236265 28.438639592526403 21.723687917199133 56-57 22.125754501550237 27.986127023027173 28.612198655982713 21.275919819439878 58-59 23.713478701345636 27.500976152004647 28.012864451989234 20.772680694660483 60-61 21.80477492135386 28.66865172000287 29.910965957506868 19.6156074011364 62-63 21.000797912751022 28.52713830515361 29.640369126671622 20.831694655423743 64-65 20.550156526322667 30.59809997973518 28.611345196186672 20.240398297755476 66-67 21.474500117495673 28.041747084543466 28.07105872597047 22.412694071990387 68-69 21.099266443585226 29.20798709773114 27.983289923589254 21.709456535094372 70-71 22.486453518570737 28.718236230269014 27.869680371943307 20.925629879216938 72-73 22.105348506038993 27.107310493839805 29.869202658119892 20.918138342001317 74-75 20.757864349398854 30.204547662065355 28.736598498871373 20.300989489664417 76-77 21.456425883627833 28.266654343132576 28.13922199020279 22.137697783036803 78-79 21.395407786296637 29.04909729190338 28.295018764794467 21.260476157005513 80-81 22.23860441433663 28.616922361692236 28.081042040247063 21.06343118372407 82-83 21.482963913260768 28.0824654742725 29.237132653066872 21.19743795939986 84-85 20.688694264211072 28.91316577925156 28.659988892206528 21.73815106433084 86-87 21.10975698849033 28.920904480116537 27.534745974713697 22.43459255667944 88-89 20.83262998791814 28.8372634891504 27.431854822864878 22.89825170006658 90-91 21.541712856859014 26.32218330223855 28.135154117047378 24.000949723855058 92-93 21.66524337227238 27.77496747085106 28.393477898719365 22.166311258157194 94-95 21.04849524334514 27.89438117550172 29.057103656832357 22.00001992432079 96-97 19.42376363439132 28.628873362600093 30.10485779498657 21.842505208022015 98-99 20.451432262192228 29.730765590728943 27.642519178648538 22.17528296843029 100-101 19.24046863770792 29.407052146834356 27.604014032454412 23.74846518300332 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 487.0 1 361.5 2 669.5 3 1020.0 4 751.5 5 574.0 6 729.0 7 1180.0 8 1688.0 9 1968.0 10 1974.0 11 1924.5 12 2067.0 13 2287.5 14 2539.5 15 2795.5 16 3013.0 17 3259.5 18 3560.5 19 3911.5 20 4178.0 21 4523.0 22 5175.5 23 6048.0 24 7149.5 25 8655.5 26 10615.0 27 13224.5 28 16978.0 29 21155.5 30 25164.5 31 29215.5 32 33245.5 33 36620.5 34 39902.5 35 43610.5 36 47236.0 37 51198.0 38 55231.0 39 57634.5 40 59292.0 41 61318.0 42 63049.0 43 65329.0 44 66371.5 45 67470.0 46 71646.0 47 89903.5 48 132773.5 49 130725.0 50 87726.5 51 65838.0 52 52338.5 53 44125.0 54 38704.0 55 33934.5 56 28755.5 57 23188.5 58 18393.0 59 14562.0 60 11348.0 61 8752.5 62 6511.5 63 4447.5 64 2855.0 65 1758.5 66 1042.5 67 635.5 68 341.0 69 167.5 70 96.5 71 57.0 72 40.0 73 25.5 74 16.5 75 9.0 76 3.5 77 1.5 78 2.0 79 2.0 80 1.5 81 1.5 82 1.5 83 1.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05080690553291629 2 0.006309354281865421 3 0.049644656059941075 4 0.0 5 0.0 6 0.0012175946859740287 7 0.0 8 0.0 9 0.0 10-11 2.7672606499409743E-4 12-13 0.0 14-15 0.0027672606499409744 16-17 0.0 18-19 0.0 20-21 0.010958352173766258 22-23 0.0 24-25 0.0 26-27 0.0076099667873376796 28-29 0.00979610270079105 30-31 0.010017483552786326 32-33 0.0063923721013636504 34-35 0.007859020245832366 36-37 0.01945384236908505 38-39 0.011650167336251502 40-41 0.0119268934012456 42-43 0.0 44-45 0.0 46-47 5.534521299881949E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0010515590469775704 58-59 8.301781949822923E-5 60-61 0.009547049242296362 62-63 0.01397466628220192 64-65 0.04178563581410871 66-67 0.02166765088903783 68-69 0.0014943207509681262 70-71 3.5974388449232664E-4 72-73 0.0 74-75 2.7672606499409744E-5 76-77 0.0 78-79 0.0 80-81 5.534521299881949E-5 82-83 0.0 84-85 8.301781949822923E-5 86-87 7.194877689846533E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 2.2138085199527796E-4 96-97 7.194877689846533E-4 98-99 7.748329819834729E-4 100-101 0.0016326837834651747 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1806841.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.1323745527464 #Duplication Level Percentage of deduplicated Percentage of total 1 80.70925353556193 46.111113028662956 2 12.27951117396405 14.031152634310976 3 3.07910457124137 5.277496669537066 4 1.2368657919715638 2.8266031879359477 5 0.6717615885311995 1.9189667343056198 6 0.38757532006103607 1.3285859011876606 7 0.2705567683172489 1.0820285437697186 8 0.20803180784864958 0.9508280931915213 9 0.15672290067317263 0.8058556316027312 >10 0.9027482320412369 9.578760899286397 >50 0.06150925655050083 2.4066319436999746 >100 0.030773364750207775 3.2854321984739547 >500 0.001959890697489476 0.8011683133386537 >1k 0.0024498633718618447 3.0704733618750377 >5k 8.819508138702642E-4 3.307240713175468 >10k+ 2.9398360462342136E-4 3.2176621456463392 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 36438 2.0166688712509844 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10882 0.6022666078531537 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10152 0.5618646023640155 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7911 0.437835980033661 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7207 0.3988729500824921 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7159 0.39621637985854874 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6782 0.37535123455799374 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6750 0.3735801877420315 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6638 0.36738152388616374 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6076 0.3362775141808272 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5403 0.2990301858326217 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5146 0.28480646609192506 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4642 0.25691247874052003 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3902 0.21595702112139364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3851 0.21313441525845386 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3780 0.20920490513553763 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3263 0.180591430015148 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2810 0.15552004852668277 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2678 0.1482144804108386 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2490 0.13780958036706054 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2288 0.12662984734129898 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2036 0.11268285366559648 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2008 0.11113318770162953 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1862 0.10305278660380189 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1850 0.10238864404781606 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 5.534521299881949E-5 0.0 0.0 5.534521299881949E-5 0.0 10-11 5.534521299881949E-5 5.534521299881949E-5 0.0 1.1069042599763898E-4 0.0 12-13 5.534521299881949E-5 1.1069042599763898E-4 0.0 1.6603563899645848E-4 0.0 14-15 5.534521299881949E-5 1.1069042599763898E-4 0.0 3.0439867149350717E-4 0.0 16-17 5.534521299881949E-5 1.1069042599763898E-4 0.0 3.5974388449232664E-4 0.0 18-19 5.534521299881949E-5 1.1069042599763898E-4 0.0 5.257795234887852E-4 0.0 20-21 5.534521299881949E-5 1.1069042599763898E-4 0.0 9.962138339787508E-4 0.0 22-23 5.534521299881949E-5 1.1069042599763898E-4 0.0 0.0024075167654486478 0.0 24-25 5.534521299881949E-5 1.1069042599763898E-4 0.0 0.0037911470904191348 0.0 26-27 5.534521299881949E-5 1.1069042599763898E-4 0.0 0.006641425559858338 0.0 28-29 5.534521299881949E-5 1.1069042599763898E-4 0.0 0.013725612823707233 0.0 30-31 5.534521299881949E-5 1.1069042599763898E-4 0.0 0.02703613654992332 0.0 32-33 5.534521299881949E-5 1.1069042599763898E-4 0.0 0.051138976810909204 0.0 34-35 5.534521299881949E-5 1.1069042599763898E-4 0.0 0.07814744075433311 0.0 36-37 5.534521299881949E-5 1.6603563899645848E-4 0.0 0.10944515870516552 0.0 38-39 5.534521299881949E-5 2.2138085199527796E-4 0.0 0.16005835599258594 0.0 40-41 5.534521299881949E-5 2.2138085199527796E-4 0.0 0.21565262244990013 0.0 42-43 5.534521299881949E-5 2.2138085199527796E-4 0.0 0.26125707796092734 0.0 44-45 5.534521299881949E-5 2.2138085199527796E-4 0.0 0.29244410548576216 0.0 46-47 5.534521299881949E-5 2.2138085199527796E-4 0.0 0.3387957213722735 0.0 48-49 5.534521299881949E-5 2.2138085199527796E-4 0.0 0.36862679117863717 0.0 50-51 5.534521299881949E-5 2.2138085199527796E-4 0.0 0.3975723375770198 0.0 52-53 5.534521299881949E-5 2.2138085199527796E-4 0.0 0.4680544663310164 0.0 54-55 5.534521299881949E-5 2.2138085199527796E-4 0.0 0.5718544133103023 0.0 56-57 5.534521299881949E-5 2.2138085199527796E-4 0.0 0.754050854502416 0.0 58-59 5.534521299881949E-5 2.2138085199527796E-4 0.0 1.0001156714951676 0.0 60-61 5.534521299881949E-5 2.2138085199527796E-4 0.0 1.2665475268714845 0.0 62-63 5.534521299881949E-5 2.2138085199527796E-4 0.0 1.3861485321619336 0.0 64-65 5.534521299881949E-5 2.2138085199527796E-4 0.0 1.54617921554802 0.0 66-67 5.534521299881949E-5 2.2138085199527796E-4 0.0 2.0649022243794555 0.0 68-69 5.534521299881949E-5 2.2138085199527796E-4 0.0 2.6531388207374085 0.0 70-71 5.534521299881949E-5 2.2138085199527796E-4 0.0 3.036515111180231 0.0 72-73 5.534521299881949E-5 2.2138085199527796E-4 0.0 3.481047861986749 0.0 74-75 5.534521299881949E-5 3.3207127799291696E-4 0.0 3.835589296457187 0.0 76-77 5.534521299881949E-5 4.427617039905559E-4 0.0 4.056997821058964 0.0 78-79 5.534521299881949E-5 4.427617039905559E-4 0.0 4.251702280388811 0.0 80-81 5.534521299881949E-5 4.427617039905559E-4 0.0 4.444082240772708 0.0 82-83 5.534521299881949E-5 4.427617039905559E-4 0.0 4.772251681249207 0.0 84-85 5.534521299881949E-5 4.427617039905559E-4 0.0 5.595068962902657 0.0 86-87 5.534521299881949E-5 4.981069169893754E-4 0.0 6.984731916090016 0.0 88-89 5.534521299881949E-5 4.981069169893754E-4 0.0 8.558888136808939 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 395 0.0 60.15481 2 TACCTGG 2365 0.0 50.63687 2 TAACGCA 500 0.0 47.49337 4 ATAACGC 530 0.0 45.723946 3 ACCTGGG 2675 0.0 43.342648 3 GTACCTG 3240 0.0 38.149975 1 GTATAAC 760 0.0 35.030014 1 GTACACG 250 0.0 34.22933 1 TACACGG 235 0.0 32.355614 2 CCTGGGG 1815 0.0 32.185593 4 TCACGCA 290 0.0 31.116348 4 TATCACG 305 0.0 28.045952 2 GAGTACG 505 0.0 25.41782 1 AGTACGC 455 0.0 25.06671 2 TACGCAG 510 0.0 23.281065 4 ACAACGC 190 3.2777461E-7 22.508072 3 CACGCAG 405 0.0 22.281458 5 GTACGCA 515 0.0 22.143866 3 GTACCCG 225 7.0927854E-8 21.129215 1 ACACGGG 360 0.0 21.118685 3 >>END_MODULE