##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003858_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2482403 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.107158265599903 31.0 31.0 34.0 28.0 34.0 2 31.06235127817683 31.0 31.0 34.0 28.0 34.0 3 31.153579414784787 31.0 31.0 34.0 28.0 34.0 4 34.65748228631693 37.0 35.0 37.0 32.0 37.0 5 34.609747087801615 37.0 35.0 37.0 32.0 37.0 6 34.74734239364036 37.0 35.0 37.0 32.0 37.0 7 34.79721100884909 37.0 35.0 37.0 32.0 37.0 8 34.778067461246216 37.0 35.0 37.0 32.0 37.0 9 36.28196348457523 39.0 37.0 39.0 32.0 39.0 10-11 36.12060813655156 39.0 37.0 39.0 32.0 39.0 12-13 36.00145262473498 39.0 35.0 39.0 32.0 39.0 14-15 37.02794509996967 40.0 37.0 41.0 31.0 41.0 16-17 37.10312789663886 40.0 37.0 41.0 32.0 41.0 18-19 37.07826267531904 40.0 37.0 41.0 31.5 41.0 20-21 36.968463823158444 39.5 37.0 41.0 31.0 41.0 22-23 36.87902387323895 39.0 36.0 41.0 31.0 41.0 24-25 36.69143185050936 39.0 36.0 41.0 30.0 41.0 26-27 36.51262023128396 39.0 36.0 41.0 30.0 41.0 28-29 36.33879168692594 39.0 36.0 40.5 30.0 41.0 30-31 36.1148566530092 39.0 35.5 40.0 29.5 41.0 32-33 35.868140668537706 38.0 35.0 40.0 29.0 41.0 34-35 35.857294524700464 39.0 35.0 40.0 29.0 41.0 36-37 35.73179294417547 38.0 35.0 40.0 28.5 41.0 38-39 35.7443503331248 38.5 35.0 40.0 28.0 41.0 40-41 35.86579213769883 39.0 35.0 40.5 29.0 41.0 42-43 35.731417501509625 39.0 35.0 41.0 27.5 41.0 44-45 35.525170772030165 39.0 35.0 40.0 27.0 41.0 46-47 35.264690704933884 39.0 35.0 40.0 26.0 41.0 48-49 35.011774881032615 39.0 35.0 40.0 24.0 41.0 50-51 34.27906609039709 37.5 33.5 39.5 24.0 40.5 52-53 34.45354601972363 38.0 34.0 40.0 24.0 40.5 54-55 34.802562073925955 38.0 34.0 40.0 24.5 41.0 56-57 34.617243453218514 38.0 34.0 40.0 23.5 41.0 58-59 34.361491868967285 38.0 34.0 40.0 23.0 41.0 60-61 34.0779206679979 38.0 34.0 40.0 22.0 41.0 62-63 33.84982011381714 38.0 33.0 40.0 22.0 41.0 64-65 33.57054193054069 37.0 33.0 40.0 21.5 41.0 66-67 33.19282948820155 37.0 33.0 39.5 20.0 41.0 68-69 32.80517023223062 36.0 32.0 39.0 18.5 41.0 70-71 32.377045548204705 36.0 32.0 39.0 18.0 40.5 72-73 31.993590686121472 35.0 31.0 38.5 15.0 40.0 74-75 31.61023290738853 35.0 31.0 37.5 12.5 40.0 76-77 31.157982607981058 35.0 31.0 37.0 8.0 39.0 78-79 30.575340708176714 35.0 30.0 36.0 7.0 39.0 80-81 30.111785636739885 34.0 30.0 36.0 4.0 38.5 82-83 29.731023931247265 34.0 29.0 35.5 2.0 37.0 84-85 29.440944318871672 34.0 29.0 35.0 2.0 37.0 86-87 29.17498850911798 34.0 29.0 35.0 2.0 36.5 88-89 28.782484552266492 34.0 29.0 35.0 2.0 36.0 90-91 28.357589198852885 34.0 28.5 35.0 2.0 36.0 92-93 28.023328202552122 34.0 27.0 35.0 2.0 35.5 94-95 27.713807749990636 34.0 27.0 35.0 2.0 35.0 96-97 27.356064063731797 34.0 26.0 35.0 2.0 35.0 98-99 27.003324198367466 33.0 25.0 35.0 2.0 35.0 100-101 26.610427476924578 33.0 25.0 35.0 2.0 35.0 102-103 26.177532817999335 33.0 24.0 35.0 2.0 35.0 104-105 25.75817383398264 32.5 21.5 35.0 2.0 35.0 106-107 25.316841785963035 32.0 19.5 35.0 2.0 35.0 108-109 24.919591621505454 32.0 18.5 34.5 2.0 35.0 110-111 24.438560539928446 32.0 13.0 34.0 2.0 35.0 112-113 23.81847427673911 31.0 4.5 34.0 2.0 35.0 114-115 23.2066453351853 31.0 2.0 34.0 2.0 35.0 116-117 22.456255893986594 31.0 2.0 34.0 2.0 35.0 118-119 21.626892370014055 30.0 2.0 34.0 2.0 35.0 120-121 20.016918284420377 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.39957084170933044 1101 2 0.39771928253976796 1101 3 0.3658864556816823 1101 4 0.6359468143231481 1101 5 0.5847575989883822 1101 6 0.6438580141470354 1101 7 0.36745404594064013 1101 8 0.447724014549145 1101 9 0.6013215967587229 1101 10-11 0.686211707412788 1101 12-13 0.6123908162185927 1101 14-15 0.7425192353238543 1101 16-17 0.5659540484349108 1101 18-19 0.4694225940031842 1101 20-21 0.7247920100636023 1101 22-23 0.6736844235608856 1101 24-25 0.6272607531796623 1101 26-27 0.6034528256430889 1101 28-29 0.5796484174544361 1101 30-31 0.5615852145307372 1101 32-33 0.6476277488526065 1101 34-35 0.6384559668397785 1101 36-37 0.4993389367737038 1101 38-39 0.5324505221270996 1101 40-41 0.5607782745176308 1101 42-43 0.47714616815140687 1101 44-45 0.6133151374921795 1101 46-47 0.659663675861843 1101 48-49 0.6153153460612018 1101 50-51 0.3551879949697181 1101 52-53 0.30113444827810554 1101 54-55 0.5446908901042846 1101 56-57 0.5659833534678569 1101 58-59 0.49915373940879704 1101 60-61 0.46963100946969405 1101 62-63 0.4769672560519993 1101 64-65 0.5537745364591231 1101 66-67 0.4583456076909229 1101 68-69 0.27815311010601107 1101 70-71 0.20913463513900865 1101 72-73 0.12412338704045212 1101 74-75 0.2301764366160448 1101 76-77 0.20188761687610324 1101 78-79 0.35259970203744473 1101 80-81 0.33605681477764904 1101 82-83 0.4812952662499761 1101 84-85 0.4877925275903472 1101 86-87 0.35997090403200005 1101 88-89 0.3359959346469985 1101 90-91 0.27962292572912517 1101 92-93 0.29935499813278454 1101 94-95 0.22165786131510856 1101 96-97 0.0776441474637295 1101 98-99 0.06296706295388788 1101 100-101 -0.038583766369175265 1101 102-103 0.007793822572232045 1101 104-105 -0.11610289075078484 1101 106-107 -0.2374993885130543 1101 108-109 -0.2902885581858463 1101 110-111 -0.3548651314729021 1101 112-113 -0.28265180857903616 1101 114-115 -0.4297666050099025 1101 116-117 -0.5563260743200402 1101 118-119 -0.4282641286883866 1101 120-121 -0.3159334143133421 1102 1 0.45954494168343274 1102 2 0.40952969435018005 1102 3 0.4889012186964443 1102 4 0.7502721186484536 1102 5 0.8405695648003473 1102 6 0.7280408683298916 1102 7 0.6534265919131883 1102 8 0.7258074126325411 1102 9 0.7882291336662632 1102 10-11 0.7326621088631882 1102 12-13 0.7466228804506514 1102 14-15 0.7354044982091139 1102 16-17 0.6622840147648716 1102 18-19 0.6038592684398694 1102 20-21 0.7981059183775088 1102 22-23 0.6598382697147329 1102 24-25 0.7304956664145763 1102 26-27 0.807804029994287 1102 28-29 0.9151068479128668 1102 30-31 0.8077584857040137 1102 32-33 0.7121978084226654 1102 34-35 0.7893378627216805 1102 36-37 0.7647986622334386 1102 38-39 0.6698553395319138 1102 40-41 0.8339510276903823 1102 42-43 0.9038503898556272 1102 44-45 0.890515344692389 1102 46-47 0.8648747610729259 1102 48-49 0.8426942634401158 1102 50-51 0.6128013075830339 1102 52-53 0.642933205076865 1102 54-55 0.7112837416971374 1102 56-57 0.8450587225432287 1102 58-59 0.7901729054279656 1102 60-61 0.9560827059213892 1102 62-63 0.8120902811750241 1102 64-65 0.6534145260991124 1102 66-67 0.7058734502187676 1102 68-69 0.6537488107017211 1102 70-71 0.43262077862515014 1102 72-73 0.504361667278733 1102 74-75 0.6414960429356533 1102 76-77 0.4955576505461359 1102 78-79 0.6783067727445165 1102 80-81 0.8427170064378409 1102 82-83 0.7473869523416674 1102 84-85 0.5282664980643155 1102 86-87 0.6081320021930985 1102 88-89 0.6768402754913403 1102 90-91 0.7968360829422814 1102 92-93 0.7845893933671775 1102 94-95 0.5537609434181903 1102 96-97 0.4966431114626957 1102 98-99 0.6455700155568387 1102 100-101 0.6868099140245043 1102 102-103 0.6446029393813468 1102 104-105 0.6701791605312657 1102 106-107 0.7015656205519534 1102 108-109 0.6001400872427993 1102 110-111 0.5180913564835841 1102 112-113 0.5682974273701973 1102 114-115 0.4733802481369551 1102 116-117 0.7067728637788981 1102 118-119 0.8259536905294311 1102 120-121 0.7411644877845589 1103 1 0.550414254932349 1103 2 0.7049453268762953 1103 3 0.680718057822066 1103 4 0.8477259710598091 1103 5 0.9903428321373511 1103 6 0.8680049332231334 1103 7 0.7705983719744864 1103 8 0.7405273480323658 1103 9 0.7221161468733897 1103 10-11 0.8881237388290728 1103 12-13 0.9804557771730629 1103 14-15 1.143101695566365 1103 16-17 1.0007301924461771 1103 18-19 0.805979543506318 1103 20-21 1.0198225869780302 1103 22-23 1.0525103599788892 1103 24-25 1.0433762859524123 1103 26-27 1.1207310305983498 1103 28-29 1.0788298105026826 1103 30-31 1.1138481310287887 1103 32-33 1.1576264748145064 1103 34-35 1.114621486872153 1103 36-37 0.8977933037266581 1103 38-39 0.8169161006153374 1103 40-41 1.0209434498102823 1103 42-43 0.9977216586560758 1103 44-45 0.9115703048408292 1103 46-47 0.9382439477565612 1103 48-49 1.0614575586906625 1103 50-51 0.8420551352787911 1103 52-53 0.8478683391904696 1103 54-55 1.004683920366439 1103 56-57 0.902149098330483 1103 58-59 0.8505765401942043 1103 60-61 0.8600214646760094 1103 62-63 0.9128422204538751 1103 64-65 0.8421398706318186 1103 66-67 0.8080860786353625 1103 68-69 0.8560676596211252 1103 70-71 0.8842453265669832 1103 72-73 0.8407728167210458 1103 74-75 0.8220635868176451 1103 76-77 0.7279892101731633 1103 78-79 0.8037303733805778 1103 80-81 0.7342627443008602 1103 82-83 0.8593864574006602 1103 84-85 0.9553935887891392 1103 86-87 0.9764861849948723 1103 88-89 1.0872287538684908 1103 90-91 0.832148818679947 1103 92-93 0.8535020670957181 1103 94-95 0.7537373912699934 1103 96-97 0.7137636522376241 1103 98-99 0.6292838937466385 1103 100-101 0.6286077290517795 1103 102-103 0.4829776587758978 1103 104-105 0.5512344445610573 1103 106-107 0.5227275401558025 1103 108-109 0.39853385589152523 1103 110-111 0.6160874743097295 1103 112-113 0.5673932324150108 1103 114-115 0.5261756585810886 1103 116-117 0.5391147428629885 1103 118-119 0.30021054793076374 1103 120-121 0.5082063851380703 1104 1 0.515104219398399 1104 2 0.613022010417346 1104 3 0.6527660298666476 1104 4 0.8064036783728241 1104 5 1.0476784240050137 1104 6 0.760506938520507 1104 7 0.6888689938226591 1104 8 0.81457253972102 1104 9 0.7966309779962515 1104 10-11 0.8882753578896185 1104 12-13 0.9689288512596193 1104 14-15 1.051217394560929 1104 16-17 0.9031507661106701 1104 18-19 0.9278590544156984 1104 20-21 0.9692663946278017 1104 22-23 0.8366717821769853 1104 24-25 0.9585670295158693 1104 26-27 0.9561141696217987 1104 28-29 0.9564381712801122 1104 30-31 0.8732119610377254 1104 32-33 0.9016408337938415 1104 34-35 0.8607214359016524 1104 36-37 0.9072475639637716 1104 38-39 0.8444332233912348 1104 40-41 0.7333619959636266 1104 42-43 0.8185954917264766 1104 44-45 0.7381166455871053 1104 46-47 0.9248843456334086 1104 48-49 1.0167768674628448 1104 50-51 0.7218668594629563 1104 52-53 0.6881969898616092 1104 54-55 0.6599570245021553 1104 56-57 0.7447790953174902 1104 58-59 0.8282899115090387 1104 60-61 0.6582227149476267 1104 62-63 0.692179054676977 1104 64-65 0.6670125049665572 1104 66-67 0.7696552107071071 1104 68-69 0.7038628018336723 1104 70-71 0.6225788578047826 1104 72-73 0.48304997830237184 1104 74-75 0.45253061714387854 1104 76-77 0.6317700741837093 1104 78-79 0.5808864618511507 1104 80-81 0.6025649096989163 1104 82-83 0.7055068430962841 1104 84-85 0.7340732993800998 1104 86-87 0.5326810393169872 1104 88-89 0.60523523382642 1104 90-91 0.6186537529635423 1104 92-93 0.7015750025683545 1104 94-95 0.5464194492503509 1104 96-97 0.41635450452738 1104 98-99 0.44709214612088743 1104 100-101 0.4515371030031865 1104 102-103 0.49741014548196816 1104 104-105 0.30695432814475865 1104 106-107 0.33136732250934386 1104 108-109 0.3230858042004918 1104 110-111 0.30591663832084137 1104 112-113 0.36865335886385253 1104 114-115 0.5500737143513774 1104 116-117 0.2538591108651467 1104 118-119 0.30898692476026923 1104 120-121 0.6022069715815306 1105 1 0.7829767218162438 1105 2 0.999964414269435 1105 3 0.8543858829439976 1105 4 1.0902919842353285 1105 5 1.158629305746686 1105 6 1.099066108839665 1105 7 0.9753730990143339 1105 8 1.0059217999634242 1105 9 1.2066480572398248 1105 10-11 1.186347255331846 1105 12-13 1.2713866161422231 1105 14-15 1.4602130115743321 1105 16-17 1.3929567212107798 1105 18-19 1.178918584839387 1105 20-21 1.354820646947907 1105 22-23 1.3173384899984555 1105 24-25 1.355584540884884 1105 26-27 1.523372642367022 1105 28-29 1.4387835835483642 1105 30-31 1.4547639086888182 1105 32-33 1.3243726896697083 1105 34-35 1.260260090551121 1105 36-37 1.3738101089768406 1105 38-39 1.311339411015986 1105 40-41 1.2756312718448513 1105 42-43 1.3651893143904346 1105 44-45 1.304888257003448 1105 46-47 1.3905838744109005 1105 48-49 1.5351817072677605 1105 50-51 1.344193340521464 1105 52-53 1.3393532340742098 1105 54-55 1.322462940505197 1105 56-57 1.4283315779810266 1105 58-59 1.3710991384160565 1105 60-61 1.434724556625092 1105 62-63 1.4837217424972096 1105 64-65 1.5972147808272013 1105 66-67 1.53436126252101 1105 68-69 1.3672691849437442 1105 70-71 1.0820479608358369 1105 72-73 1.1567388891404882 1105 74-75 1.1138438669329638 1105 76-77 1.209569938372855 1105 78-79 1.3112537684750158 1105 80-81 1.3690904153885697 1105 82-83 1.4585804896176775 1105 84-85 1.4004843947152033 1105 86-87 1.3633347833752616 1105 88-89 1.4265266715694906 1105 90-91 1.462276610341572 1105 92-93 1.3373656892362575 1105 94-95 1.4119938674243144 1105 96-97 1.2585357091284877 1105 98-99 1.3019819541337263 1105 100-101 1.3639000060630515 1105 102-103 1.3749721349051818 1105 104-105 1.2993896636592979 1105 106-107 1.0740851857519296 1105 108-109 1.2073498534216398 1105 110-111 1.162345635995596 1105 112-113 1.2950438767970596 1105 114-115 1.0117454224815106 1105 116-117 1.296676980280953 1105 118-119 0.9339485479057323 1105 120-121 0.6714606292147209 1106 1 0.4400498169251499 1106 2 0.7065747545531345 1106 3 0.6080919694661446 1106 4 0.9169855222039587 1106 5 0.8634794034996602 1106 6 0.9003130406020574 1106 7 0.7572730378648984 1106 8 0.6958665932180352 1106 9 0.7032848381956498 1106 10-11 0.9104375497965336 1106 12-13 0.9186759735563754 1106 14-15 0.9918731757304258 1106 16-17 0.8418047863909166 1106 18-19 0.6946690434601663 1106 20-21 0.846976638300859 1106 22-23 0.7270094026753426 1106 24-25 0.9542003243297543 1106 26-27 0.9877468941476835 1106 28-29 1.0548238560509304 1106 30-31 0.9749728502867967 1106 32-33 1.0361560139598183 1106 34-35 0.9659288156284163 1106 36-37 0.9351896747297062 1106 38-39 1.0613059941404615 1106 40-41 1.1659216886084138 1106 42-43 1.1263121386331676 1106 44-45 1.1411959506361526 1106 46-47 1.167369050409313 1106 48-49 1.269578782429896 1106 50-51 1.06285856027187 1106 52-53 1.1699690347442768 1106 54-55 1.215998325359088 1106 56-57 1.14223230919049 1106 58-59 1.1217170922353077 1106 60-61 1.1258938888904595 1106 62-63 1.1730714053140474 1106 64-65 1.0184807464284873 1106 66-67 1.0598089883648285 1106 68-69 0.8049353434672 1106 70-71 0.8537405944818062 1106 72-73 0.916131184311407 1106 74-75 0.8272956234720787 1106 76-77 0.8761872548599534 1106 78-79 0.8939405541677701 1106 80-81 0.7284029579132643 1106 82-83 0.7847706213042791 1106 84-85 0.8892461616755583 1106 86-87 0.7745211685822646 1106 88-89 0.6435008079413471 1106 90-91 0.6395533072384509 1106 92-93 0.7398840986618822 1106 94-95 0.7438661598392002 1106 96-97 0.7632902070571603 1106 98-99 1.09021017072331 1106 100-101 0.9237790852041989 1106 102-103 0.7956857746747126 1106 104-105 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-0.7801154779312576 2316 80-81 -0.7565799380509439 2316 82-83 -0.6956660418283782 2316 84-85 -0.8317843975620995 2316 86-87 -0.5152211165461331 2316 88-89 -0.49783206528104884 2316 90-91 -0.4072489826046599 2316 92-93 -0.38082290196255286 2316 94-95 -0.35628810310487324 2316 96-97 -0.28940629054105216 2316 98-99 -0.16245088534895658 2316 100-101 0.11488762391376994 2316 102-103 0.09949349699167342 2316 104-105 -0.17613553874603483 2316 106-107 -0.31666870839145744 2316 108-109 -0.23732712419235824 2316 110-111 0.10053042192410899 2316 112-113 -0.027220459308448852 2316 114-115 0.04272813353632188 2316 116-117 0.05115553307657095 2316 118-119 0.10057412432907853 2316 120-121 -0.3752499649582077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30244.0 3 4184.0 4 3498.0 5 4122.0 6 4851.0 7 4859.0 8 6012.0 9 7554.0 10 10364.0 11 12992.0 12 14065.0 13 16840.0 14 21696.0 15 21337.0 16 15600.0 17 15046.0 18 16417.0 19 17667.0 20 19609.0 21 22325.0 22 25699.0 23 29067.0 24 33157.0 25 38302.0 26 44050.0 27 51452.0 28 61482.0 29 73538.0 30 87774.0 31 105199.0 32 129039.0 33 162589.0 34 206365.0 35 270442.0 36 361698.0 37 396731.0 38 136005.0 39 532.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.93191086513254 19.40750720134641 14.094734116580899 21.565847816940156 2 14.243924511257793 21.448807729379123 39.97846451417414 24.32880324518894 3 20.428906189716162 26.052934033488878 30.65670041546353 22.86145936133143 4 11.41899378735212 16.557573473970912 41.485594704408946 30.537838034268027 5 11.19980638459783 38.20887073654255 35.96004083465781 14.631282044201813 6 27.154378895495633 37.85259223189385 19.15214196163829 15.840886910972227 7 25.38519226216989 33.08002359857093 23.71887816649011 17.81590597276907 8 23.498095061776514 32.70984364241078 21.47781307245586 22.31424822335685 9 23.228248954569445 19.11400267955016 24.614510170110837 33.04323819576956 10-11 23.49045778555345 25.594984001752163 29.16928263341126 21.745275579283128 12-13 26.23182065926214 23.75381754737108 28.620242719382738 21.39411907398404 14-15 22.099445046443712 25.93519763191667 29.210272142013544 22.75508517962607 16-17 19.66249643399929 29.295134909040332 29.52274981548167 21.519618841478703 18-19 19.036649686788575 30.182479698582622 30.474355414892667 20.306515199736133 20-21 20.918564840934355 27.69055768731638 28.965755486153167 22.425121985596093 22-23 19.84831167860788 27.630815136914 29.857166322951073 22.663706861527046 24-25 21.54336599257701 27.4325668403273 29.61211911788979 21.411948049205897 26-27 20.981470784991874 26.160636609944444 31.867448048052317 20.99044455701137 28-29 19.601644580260814 28.963510723157178 30.675593018778724 20.759251677803285 30-31 20.296174412150634 27.71783799218631 29.830787362028204 22.155200233634847 32-33 20.44598333731031 27.683622129595314 30.492579975325818 21.37781455776856 34-35 21.71920501716435 26.614384904636424 30.34382719233858 21.32258288586065 36-37 21.5017367793625 26.537472733650702 31.427063696263787 20.533726790723012 38-39 20.966913171446606 27.792615092656604 30.96544681597767 20.27502491991912 40-41 20.092856191116873 28.41924298114765 30.674736843815708 20.813163983919768 42-43 19.635189341240746 28.60863383871721 29.058990789353427 22.697186030688616 44-45 20.35630736739501 27.45638047274795 29.09199090244497 23.09532125741207 46-47 21.45586976628962 28.13664224209264 29.284817201506293 21.122670790111446 48-49 21.217107500934627 27.56032038742486 29.77081735427146 21.451754757369052 50-51 19.39914993096211 28.05493941384118 31.325123271792744 21.22078738340397 52-53 20.03350377653643 28.96709956762704 29.172959540199354 21.826437115637173 54-55 19.414566220590356 28.013320342830657 29.82731933584329 22.744794100735703 56-57 20.849041843529218 26.96919391315126 29.49612206208358 22.68564218123594 58-59 22.021271409535572 26.614287107153306 29.3716077870584 21.992833696252724 60-61 20.828938408205524 27.201979382180525 30.995009694568274 20.974072515045677 62-63 19.91746568720641 27.513340027719963 30.547546393561692 22.02164789151194 64-65 19.36891517088953 29.111648205158826 29.997183200992524 21.522253422959125 66-67 19.860649609949295 27.56407743782453 28.961521347028047 23.61375160519813 68-69 19.45778636860923 28.05947823698914 29.21113579756404 23.27159959683759 70-71 21.0772484523921 27.470702964309286 28.712628720165394 22.73941986313322 72-73 20.151903085295253 26.519167982603424 30.85943316900122 22.4694957631001 74-75 19.503681108977 28.73036582734646 29.67422012865089 22.091732935025654 76-77 20.0254476399163 27.13071154640943 29.240504580128164 23.603336233546102 78-79 20.233423883657604 27.16960989098999 29.78165880280058 22.81530742255183 80-81 21.069515106958832 26.677087128506738 29.371490820017698 22.881906944516732 82-83 20.161277373056517 25.99357256435007 30.874557684415795 22.970592378177614 84-85 20.14053368502989 27.055406227199036 30.171728400153853 22.632331687617224 86-87 20.327179744084958 27.713823056731616 29.489711818787757 22.469285380395664 88-89 19.60992002281754 27.39425201964275 28.7286880382015 24.26713991933821 90-91 19.68655836106801 26.198303635337062 29.46661040155532 24.648527602039604 92-93 20.661536792605766 26.909472308796804 29.695169443079973 22.733821455517457 94-95 19.977317685258203 26.353757979742333 29.90821442689473 23.760709908104733 96-97 18.77563060480609 26.648637293903832 31.002503281976978 23.5732288193131 98-99 20.06824647092463 27.225346668579675 29.27355368978441 23.432853170711283 100-101 19.454817740876884 25.932590845083304 30.06996133127696 24.542630082762855 102-103 20.681576945305842 25.593721146843457 29.668007772386495 24.056694135464205 104-105 21.642728766268483 25.563739788779888 28.993425981146515 23.800105463805117 106-107 20.573863914511925 25.61788299320391 30.62342943165104 23.184823660633125 108-109 20.357508995006913 26.014801110459068 30.185989909829537 23.441699984704474 110-111 20.144851997544635 27.10618566291848 28.98549164407586 23.763470695461024 112-113 20.7719544871886 25.817692790554524 28.34911438537187 25.061238336885 114-115 19.994575888299863 25.72020319088099 29.46615161306378 24.819069307755363 116-117 21.1653425415521 25.678815210630283 28.87236235882507 24.283479888992545 118-119 20.66147558688184 25.29431211622893 30.22344698886961 23.820765308019624 120-121 20.031431604771136 27.709645268986847 28.821859267183676 23.437063859058345 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5620.0 1 6198.0 2 4293.0 3 1729.0 4 1494.0 5 1355.0 6 1426.0 7 1589.0 8 1912.0 9 2070.5 10 1917.0 11 1829.0 12 1866.5 13 2048.0 14 2282.0 15 2467.0 16 2688.5 17 2954.0 18 3256.0 19 3585.5 20 3989.0 21 4583.0 22 5438.5 23 6648.0 24 8245.0 25 10229.0 26 13064.0 27 16872.5 28 22073.0 29 28355.5 30 34834.0 31 41480.5 32 48149.5 33 53945.5 34 59169.0 35 64700.5 36 69982.0 37 75455.5 38 80440.5 39 83328.0 40 85501.5 41 87232.5 42 88555.0 43 90922.5 44 92225.5 45 93651.5 46 100715.0 47 126371.0 48 180708.0 49 177907.5 50 119327.0 51 86574.5 52 68943.5 53 58564.5 54 51205.0 55 44022.0 56 36417.0 57 28681.5 58 22156.0 59 17461.5 60 13546.0 61 10065.0 62 7237.0 63 4942.0 64 3192.0 65 2038.0 66 1258.5 67 741.0 68 458.5 69 280.5 70 163.0 71 111.0 72 93.5 73 73.0 74 58.0 75 47.5 76 34.5 77 24.5 78 20.0 79 17.5 80 14.5 81 11.5 82 9.5 83 11.0 84 11.0 85 7.0 86 5.0 87 4.5 88 3.0 89 1.5 90 2.0 91 3.0 92 3.5 93 3.5 94 2.0 95 3.0 96 4.5 97 3.0 98 1.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4287377996239934 2 0.8225900468215676 3 0.8094173266790284 4 0.7864959879600533 5 0.7971711281367289 6 0.80986044570523 7 0.7872210918211103 8 0.7787212632276065 9 0.7775933238881841 10-11 0.7720140525128273 12-13 0.7762841085834975 14-15 0.7780364429143858 16-17 0.802065579198865 18-19 0.8285318701274531 20-21 0.8230734493956058 22-23 0.7997694169721838 24-25 0.7915515732135354 26-27 0.7923975277181021 28-29 0.8159432614285432 30-31 0.8234561431000527 32-33 0.8183804160726522 34-35 0.8304050551018509 36-37 0.8381596380603795 38-39 0.8264975509617092 40-41 0.8357627669641069 42-43 0.8289548473797365 44-45 0.8138485169410447 46-47 0.8081081113743418 48-49 0.8135866738801073 50-51 0.8489153453327279 52-53 0.8174538944724123 54-55 0.830022361397404 56-57 0.8736695854782645 58-59 0.8412413294698726 60-61 0.8552600041169786 62-63 0.8314121437977637 64-65 0.8213211150647175 66-67 0.8249869179178402 68-69 0.8404759420609788 70-71 0.8501641353156598 72-73 0.8551592952473872 74-75 0.8590063740657742 76-77 0.8727027803301881 78-79 0.9115764039924219 80-81 0.9321210133890429 82-83 0.9055540135908634 84-85 0.8688959850596377 86-87 0.8522588798031585 88-89 0.8515337759421013 90-91 0.8634577061017087 92-93 0.851352499976837 94-95 0.8465184742364555 96-97 0.8553808547604881 98-99 0.8884939310821007 100-101 0.8764894338268202 102-103 0.8510100898202265 104-105 0.8417247320439106 106-107 0.8424699776788861 108-109 0.8418657244613384 110-111 0.8401536736782866 112-113 0.8507079632114528 114-115 0.8528228494728696 116-117 0.8800948113581879 118-119 0.8744551146610763 120-121 0.8534472444643356 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2482403.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.380685898358834 #Duplication Level Percentage of deduplicated Percentage of total 1 82.94564462893935 50.083149149768815 2 11.086796467272007 13.388567502187707 3 2.6601384205618595 4.818629472543059 4 1.0430998868393482 2.519323465114413 5 0.5470602839832752 1.651593758733056 6 0.34845909146867654 1.2624119370238587 7 0.23575283944047865 0.9964442703531233 8 0.1664548631011518 0.8040527044131971 9 0.1323042072146082 0.7189756900970974 >10 0.7525608200011328 8.414049920978407 >50 0.05146769189639577 2.121454916278795 >100 0.02469022449700467 2.65503805069856 >500 0.002038015165135465 0.8232496811968295 >1k 0.0027852873923508133 3.986218436406857 >5k 4.75536871864773E-4 2.019525401297131 >10k+ 2.7173535535129884E-4 3.73731564290905 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 32108 1.2934241539347155 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 27237 1.0972029924230675 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 17053 0.686955341256033 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 14714 0.5927321228664322 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9624 0.38768886437858796 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8797 0.3543743703177929 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6875 0.27694939137601754 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6719 0.2706651579135217 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5927 0.23876058802700448 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 5861 0.23610187386979473 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5431 0.21877994830009473 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4968 0.20012866565178983 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4652 0.18739906453545213 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4651 0.18735878098761563 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4395 0.17704619274146866 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4373 0.1761599546890654 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3997 0.161013340702537 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3990 0.16073135586768145 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3980 0.16032852038931633 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3901 0.1571461201102319 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3882 0.15638073270133818 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3108 0.12520126667587816 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3099 0.12483871474534958 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3066 0.12350935766674469 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3030 0.12205914994463027 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2951 0.11887674966554584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2556 0.10296474827012375 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.056709567302327E-5 0.0 10-11 0.0 0.0 0.0 1.8127596526430236E-4 0.0 12-13 0.0 0.0 0.0 2.8198483485558147E-4 0.0 14-15 0.0 2.0141773918255818E-5 0.0 3.021266087738373E-4 0.0 16-17 0.0 4.0283547836511636E-5 0.0 4.834025740381397E-4 0.0 18-19 0.0 4.0283547836511636E-5 0.0 7.452456349754653E-4 0.0 20-21 0.0 4.0283547836511636E-5 0.0 0.001329357078604884 0.0 22-23 0.0 4.0283547836511636E-5 0.0 0.0023163040005994193 0.0 24-25 0.0 6.0425321754767454E-5 0.0 0.004572182679444071 0.0 26-27 0.0 8.056709567302327E-5 0.0 0.008419261497830932 0.0 28-29 0.0 8.056709567302327E-5 0.0 0.017845611691574656 0.0 30-31 0.0 8.056709567302327E-5 0.0 0.04264013538494757 0.0 32-33 0.0 8.056709567302327E-5 0.0 0.0951497399898405 0.0 34-35 0.0 1.2085064350953491E-4 0.0 0.15438669708343083 0.0 36-37 0.0 1.2085064350953491E-4 0.0 0.2251648906321818 0.0 38-39 0.0 1.2085064350953491E-4 0.0 0.3367100345914825 0.0 40-41 0.0 1.2085064350953491E-4 0.0 0.5783911798366341 0.0 42-43 0.0 1.2085064350953491E-4 0.0 0.7890539932476717 0.0 44-45 0.0 1.2085064350953491E-4 0.0 1.0269686267701095 0.0 46-47 0.0 1.2085064350953491E-4 0.0 1.4287164493436402 0.0 48-49 0.0 1.2085064350953491E-4 0.0 1.7004088377269926 0.0 50-51 0.0 1.2085064350953491E-4 0.0 2.0460416781642623 0.0 52-53 0.0 1.2085064350953491E-4 0.0 2.417959533564856 0.0 54-55 0.0 1.2085064350953491E-4 0.0 2.8411784871352475 0.0 56-57 0.0 1.2085064350953491E-4 0.0 3.631783396974625 0.0 58-59 0.0 1.2085064350953491E-4 0.0 4.33845753489663 0.0 60-61 0.0 1.2085064350953491E-4 0.0 4.90073529559866 0.0 62-63 0.0 1.2085064350953491E-4 0.0 5.483275680862455 0.0 64-65 0.0 1.2085064350953491E-4 0.0 6.073530365536941 0.0 66-67 0.0 1.2085064350953491E-4 0.0 6.786629729338871 0.0 68-69 0.0 1.2085064350953491E-4 0.0 7.5566296044598715 0.0 70-71 0.0 1.2085064350953491E-4 0.0 8.120619415944955 0.0 72-73 0.0 1.2085064350953491E-4 0.0 8.702535406217283 0.0 74-75 0.0 1.2085064350953491E-4 0.0 9.476744912087199 0.0 76-77 0.0 1.2085064350953491E-4 0.0 10.265194652117323 0.0 78-79 0.0 1.2085064350953491E-4 0.0 11.089396040852352 0.0 80-81 0.0 1.2085064350953491E-4 0.0 11.878369467004351 0.0 82-83 0.0 1.2085064350953491E-4 0.0 12.743700358080456 0.0 84-85 0.0 1.2085064350953491E-4 0.0 13.734192232284604 0.0 86-87 0.0 1.4099241742779073E-4 0.0 14.63545604803088 0.0 88-89 0.0 1.6113419134604654E-4 0.0 15.56795975512437 0.0 90-91 0.0 1.6113419134604654E-4 0.0 16.55472540115364 0.0 92-93 0.0 2.014177391825582E-4 0.0 17.516132553819826 0.0 94-95 0.0 2.014177391825582E-4 0.0 18.360294440507847 0.0 96-97 0.0 2.014177391825582E-4 0.0 19.19517096941955 2.0141773918255818E-5 98-99 0.0 2.014177391825582E-4 0.0 20.064751774792406 4.0283547836511636E-5 100-101 0.0 2.014177391825582E-4 0.0 20.856766608806062 4.0283547836511636E-5 102-103 0.0 2.8198483485558147E-4 0.0 21.60732967209595 4.0283547836511636E-5 104-105 0.0 3.222683826920931E-4 0.0 22.353058709645452 4.0283547836511636E-5 106-107 0.0 3.222683826920931E-4 0.0 23.187471977757035 4.0283547836511636E-5 108-109 0.0 3.222683826920931E-4 0.0 24.113046914622643 4.0283547836511636E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3025 0.0 60.480682 2 TATAACG 430 0.0 53.518814 2 ACCTGGG 3400 0.0 52.890778 3 TAACGCA 520 0.0 46.405483 4 ATAACGC 565 0.0 40.67486 3 GTACCTG 5055 0.0 37.16899 1 GTACACG 440 0.0 34.056892 1 CCTGGGG 2290 0.0 33.870502 4 TACACGG 435 0.0 31.742195 2 ACACGGG 455 0.0 31.567715 3 GTATAAC 1105 0.0 25.557486 1 GTACGCA 585 0.0 23.570562 3 AGTACGC 585 0.0 22.619705 2 GTACTAG 690 0.0 22.552725 1 GTACGTG 285 4.629328E-9 22.244987 1 GAGTACT 6995 0.0 21.689077 12-13 AGTACTT 7420 0.0 21.608526 12-13 GAGTACG 760 0.0 21.23385 1 GTACATG 8950 0.0 20.735628 1 GTACCGG 200 6.276824E-5 20.17216 1 >>END_MODULE