##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003857_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1823033 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.854201213033445 31.0 31.0 34.0 28.0 34.0 2 30.778006212723522 31.0 31.0 34.0 27.0 34.0 3 30.8609059737262 31.0 31.0 34.0 27.0 34.0 4 34.336479372562096 37.0 35.0 37.0 30.0 37.0 5 34.2948602685744 37.0 35.0 37.0 30.0 37.0 6 34.41111159260419 37.0 35.0 37.0 30.0 37.0 7 34.45743000812382 37.0 35.0 37.0 30.0 37.0 8 34.43606232032004 37.0 35.0 37.0 30.0 37.0 9 35.906679144041824 39.0 35.0 39.0 30.0 39.0 10-11 35.73328239258423 39.0 35.0 39.0 30.0 39.0 12-13 35.60092439357928 39.0 35.0 39.0 30.0 39.0 14-15 36.59417849265482 39.0 36.0 41.0 30.0 41.0 16-17 36.68343880774511 39.0 36.0 41.0 30.0 41.0 18-19 36.66222827562639 39.0 36.0 41.0 30.0 41.0 20-21 36.542528303108064 39.0 36.0 41.0 30.0 41.0 22-23 36.45081822435469 39.0 36.0 41.0 30.0 41.0 24-25 36.25874353344125 39.0 36.0 41.0 29.0 41.0 26-27 36.07679592196082 39.0 35.5 41.0 28.0 41.0 28-29 35.894868880596235 39.0 35.0 40.0 27.0 41.0 30-31 35.67710787462432 38.5 35.0 40.0 27.0 41.0 32-33 35.42818917704726 38.0 35.0 40.0 27.0 41.0 34-35 35.38317792382255 38.0 35.0 40.0 26.5 41.0 36-37 35.24282281231333 38.0 35.0 40.0 25.0 41.0 38-39 35.251892587791886 38.0 35.0 40.0 25.5 41.0 40-41 35.351966749916215 39.0 35.0 40.0 26.0 41.0 42-43 35.202645810580506 39.0 35.0 40.0 25.0 41.0 44-45 34.9826124924782 38.5 34.5 40.0 24.0 41.0 46-47 34.70096811193215 38.0 34.0 40.0 23.5 41.0 48-49 34.43081611797483 38.0 34.0 40.0 22.5 41.0 50-51 33.67301058181613 37.5 33.0 39.5 21.0 40.5 52-53 33.82815725222747 38.0 33.0 40.0 22.0 40.5 54-55 34.162658328181664 38.0 34.0 40.0 22.0 41.0 56-57 33.95223262552022 38.0 33.5 40.0 20.0 41.0 58-59 33.68331291863614 38.0 33.0 40.0 18.5 41.0 60-61 33.393490682834596 38.0 33.0 40.0 18.0 41.0 62-63 33.15662333046083 37.0 32.0 40.0 17.0 41.0 64-65 32.8684486238044 37.0 32.0 40.0 13.5 41.0 66-67 32.48500767676723 36.0 31.5 39.0 12.5 41.0 68-69 32.09880841432931 36.0 31.0 39.0 8.0 41.0 70-71 31.661358845396656 35.0 31.0 39.0 7.5 40.0 72-73 31.289908081751673 35.0 31.0 38.0 7.0 40.0 74-75 30.91573740025551 35.0 30.0 37.0 4.0 39.5 76-77 30.48735212143719 35.0 30.0 37.0 2.0 39.0 78-79 29.913171895407267 34.0 29.0 36.0 2.0 39.0 80-81 29.4574220543457 34.0 29.0 36.0 2.0 38.5 82-83 29.101246384459305 34.0 29.0 35.0 2.0 37.0 84-85 28.84472195511546 34.0 29.0 35.0 2.0 37.0 86-87 28.63672105770987 34.0 29.0 35.0 2.0 36.5 88-89 28.280391249088744 34.0 28.0 35.0 2.0 36.0 90-91 27.88424455289619 34.0 27.0 35.0 2.0 36.0 92-93 27.60724298463056 34.0 26.0 35.0 2.0 35.0 94-95 27.369596710536783 34.0 26.0 35.0 2.0 35.0 96-97 27.077624211958863 34.0 25.0 35.0 2.0 35.0 98-99 26.832996166278942 33.5 25.0 35.0 2.0 35.0 100-101 26.52918158914293 33.0 25.0 35.0 2.0 35.0 102-103 26.17496913111282 33.0 24.0 35.0 2.0 35.0 104-105 25.84687166935541 33.0 23.0 35.0 2.0 35.0 106-107 25.489652408925124 32.0 19.5 35.0 2.0 35.0 108-109 25.177275726769622 32.0 19.0 35.0 2.0 35.0 110-111 24.807801339855065 32.0 17.5 35.0 2.0 35.0 112-113 24.29672062985146 32.0 8.5 34.0 2.0 35.0 114-115 23.777136233957368 31.0 2.0 34.0 2.0 35.0 116-117 23.14029916079413 31.0 2.0 34.0 2.0 35.0 118-119 22.4023572255686 31.0 2.0 34.0 2.0 35.0 120-121 20.771874672592325 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5753711895307418 1101 2 0.4101517881591761 1101 3 0.49862121200483855 1101 4 0.6118778193334862 1101 5 0.6740872951567454 1101 6 0.6474425756309117 1101 7 0.5599957343902204 1101 8 0.4711205640727414 1101 9 0.5820156437926443 1101 10-11 0.6834436685456424 1101 12-13 0.6218281075915115 1101 14-15 0.8622927889776264 1101 16-17 0.6915012897864727 1101 18-19 0.7331774624634946 1101 20-21 0.7847426347821624 1101 22-23 0.6829089349440451 1101 24-25 0.7566971929780735 1101 26-27 0.7550060093068822 1101 28-29 0.8592742910403288 1101 30-31 0.7065773889245506 1101 32-33 0.8653047811278896 1101 34-35 0.8633854423237111 1101 36-37 0.8823068215632546 1101 38-39 0.8168162175344875 1101 40-41 0.8662457176894094 1101 42-43 0.9147563577582858 1101 44-45 0.9714342354930139 1101 46-47 0.8288882203029431 1101 48-49 0.969400795139336 1101 50-51 0.728293845054246 1101 52-53 0.7391747717720349 1101 54-55 0.990462384139569 1101 56-57 0.8664792838151101 1101 58-59 0.9043284983676259 1101 60-61 0.6251395849414152 1101 62-63 0.6252550255410014 1101 64-65 0.6913785942528037 1101 66-67 0.6756738082175602 1101 68-69 0.6480818341463461 1101 70-71 0.5384991189626263 1101 72-73 0.4765704027576412 1101 74-75 0.4870471979663549 1101 76-77 0.4329330148894748 1101 78-79 0.6571922177172631 1101 80-81 0.8212576152280313 1101 82-83 0.9151046993169949 1101 84-85 0.8634220999983135 1101 86-87 0.6625284483849647 1101 88-89 0.610242933627962 1101 90-91 0.6252475289753185 1101 92-93 0.7002505521019842 1101 94-95 0.6710053962684981 1101 96-97 0.5903409582367729 1101 98-99 0.7200824263582959 1101 100-101 0.7312868067640927 1101 102-103 0.7620857604214635 1101 104-105 0.677927843244877 1101 106-107 0.5730205905995014 1101 108-109 0.6694626458935602 1101 110-111 0.5037515559399246 1101 112-113 0.521784631692146 1101 114-115 0.5762985236399736 1101 116-117 0.6923277969032746 1101 118-119 0.67691771161304 1101 120-121 0.4770834678195115 1102 1 0.5814113995677275 1102 2 0.5732262844750586 1102 3 0.5487173142755353 1102 4 0.7193200403428293 1102 5 0.8069936771944413 1102 6 0.7931451455726588 1102 7 0.5622712526602385 1102 8 0.7955586539783539 1102 9 0.7216850613917103 1102 10-11 0.7139705489506554 1102 12-13 0.8238604882929579 1102 14-15 0.8606177628641518 1102 16-17 0.7409048558041462 1102 18-19 0.7933669497925777 1102 20-21 0.9838219585609451 1102 22-23 0.7978173143092349 1102 24-25 0.9351818707237456 1102 26-27 1.0029134029474207 1102 28-29 0.9554827212209176 1102 30-31 0.9953679471411263 1102 32-33 0.8324171019800772 1102 34-35 0.904467986763656 1102 36-37 0.9051343456299961 1102 38-39 0.7625675443810209 1102 40-41 0.7646046273226901 1102 42-43 1.0448437790649976 1102 44-45 0.8587384432903562 1102 46-47 0.9234013334989513 1102 48-49 0.8406416907136887 1102 50-51 0.6882364307111359 1102 52-53 0.8087806936480604 1102 54-55 0.6868709587676278 1102 56-57 0.5907151707790774 1102 58-59 0.598906483537057 1102 60-61 0.790721915297965 1102 62-63 0.7795776868563635 1102 64-65 0.669243606439089 1102 66-67 0.6631325795200311 1102 68-69 0.38561971043908017 1102 70-71 0.3106220498819141 1102 72-73 0.44535451494675016 1102 74-75 0.45688849394723263 1102 76-77 0.40240613448446183 1102 78-79 0.4231990683858591 1102 80-81 0.4861894614267612 1102 82-83 0.38647545646643877 1102 84-85 0.2738152606220261 1102 86-87 0.20828377459159597 1102 88-89 0.2285243027265551 1102 90-91 0.30635355201162895 1102 92-93 0.29412582617577954 1102 94-95 0.1205893451896145 1102 96-97 0.16730570270118506 1102 98-99 0.11024411813466628 1102 100-101 0.17200041023459178 1102 102-103 0.07378000698907528 1102 104-105 0.05204916030473683 1102 106-107 -0.15254096661016803 1102 108-109 -0.0589834497899524 1102 110-111 -0.21945126102112766 1102 112-113 -0.3964902090392215 1102 114-115 -0.5006303442117606 1102 116-117 -0.2336958040264605 1102 118-119 -0.296773262536977 1102 120-121 -0.48483380188792324 1103 1 0.49148144452099274 1103 2 0.46732685016105435 1103 3 0.5532469734842174 1103 4 0.619622937438038 1103 5 0.8447781646277477 1103 6 0.8274617179583288 1103 7 0.5226745165047859 1103 8 0.4773270724640639 1103 9 0.6019393152669679 1103 10-11 0.7879764272078447 1103 12-13 0.565198722313049 1103 14-15 0.705509903903824 1103 16-17 0.6323411142549418 1103 18-19 0.5572644396612461 1103 20-21 0.6491899753229617 1103 22-23 0.8415631631109548 1103 24-25 0.6242607298541358 1103 26-27 0.7964792461660863 1103 28-29 0.5667032296644905 1103 30-31 0.6160451660067352 1103 32-33 0.6368639778724798 1103 34-35 0.5947431931604328 1103 36-37 0.40803128040123227 1103 38-39 0.48371820039066904 1103 40-41 0.49888654622713346 1103 42-43 0.5633549949208856 1103 44-45 0.7205495048099095 1103 46-47 0.6945662680263283 1103 48-49 0.8734232422270551 1103 50-51 0.41689437839079346 1103 52-53 0.5535245947957961 1103 54-55 0.35941449220342747 1103 56-57 0.5213292753876715 1103 58-59 0.5043313576775432 1103 60-61 0.3600168453011463 1103 62-63 0.35657756171880095 1103 64-65 0.2672624219959374 1103 66-67 0.39360454068246753 1103 68-69 0.5140455118810507 1103 70-71 0.34973139124116415 1103 72-73 0.29499835416742926 1103 74-75 0.23484823815320155 1103 76-77 0.16450340808973607 1103 78-79 0.3170607496572586 1103 80-81 0.374241229877331 1103 82-83 0.2951859940125239 1103 84-85 0.3382144238046294 1103 86-87 0.4369314545731129 1103 88-89 0.5097831866919407 1103 90-91 0.5649707176777312 1103 92-93 0.4518732857226553 1103 94-95 0.5386473384321846 1103 96-97 0.5239089556363048 1103 98-99 0.43872783581147345 1103 100-101 0.37174965379401925 1103 102-103 0.3830029668455808 1103 104-105 0.6451330634418397 1103 106-107 0.6249504743008352 1103 108-109 0.4147894800656253 1103 110-111 0.6222776268267296 1103 112-113 0.4777256757315378 1103 114-115 0.6599938355756336 1103 116-117 0.3721994590351727 1103 118-119 0.09358071635648457 1103 120-121 0.005411403578342799 1104 1 0.6078763270622183 1104 2 0.7924450326670822 1104 3 0.6874671005324657 1104 4 0.9941409140087814 1104 5 0.9090503676417683 1104 6 0.9137078073263396 1104 7 0.7066632130923765 1104 8 0.7713809380271428 1104 9 0.8418710210755904 1104 10-11 1.079595585110198 1104 12-13 1.1027794433496965 1104 14-15 1.258468825408272 1104 16-17 1.1178962019419174 1104 18-19 0.9855843236005981 1104 20-21 1.0728345362959288 1104 22-23 1.0414860196240738 1104 24-25 1.0703359521556806 1104 26-27 1.2611529571790214 1104 28-29 1.2266738197381457 1104 30-31 1.18481373225638 1104 32-33 1.1492932904236781 1104 34-35 0.9442222690176507 1104 36-37 0.9389324281778855 1104 38-39 1.1117729344347964 1104 40-41 1.1839595282423332 1104 42-43 1.110247872389401 1104 44-45 1.1358638850795941 1104 46-47 1.2092452425607618 1104 48-49 1.232355273716074 1104 50-51 1.0217521152739337 1104 52-53 1.0946021034394917 1104 54-55 0.9997702404623539 1104 56-57 1.0562797160431074 1104 58-59 1.046793994899197 1104 60-61 0.9835541620236796 1104 62-63 1.1731616020636935 1104 64-65 1.2026000604512248 1104 66-67 1.0650344749871365 1104 68-69 1.13649525347504 1104 70-71 1.0897351415002277 1104 72-73 0.9601770342736273 1104 74-75 1.0410817279666418 1104 76-77 0.882934944478766 1104 78-79 0.9159211972539687 1104 80-81 0.9534700581845499 1104 82-83 1.076869103701025 1104 84-85 1.1559743677481258 1104 86-87 0.9858392738632631 1104 88-89 1.034957294113834 1104 90-91 1.2772536385373954 1104 92-93 1.2395397034686155 1104 94-95 1.3719823714443322 1104 96-97 1.3219779011884647 1104 98-99 1.4738782902836576 1104 100-101 1.3514513766103988 1104 102-103 1.415217363234806 1104 104-105 1.480873361291362 1104 106-107 1.2301253383539432 1104 108-109 1.2148577413599924 1104 110-111 1.219971381987449 1104 112-113 0.9252985343829572 1104 114-115 1.194543755142444 1104 116-117 1.0664727476505043 1104 118-119 0.9241303595884212 1104 120-121 1.0201790355529177 1105 1 0.7714785473937411 1105 2 0.9541276090670685 1105 3 0.9196279315853957 1105 4 1.1737677726020905 1105 5 1.1083103987014908 1105 6 1.0616591253827465 1105 7 1.0312076161836288 1105 8 1.0013191724926784 1105 9 1.058414218018882 1105 10-11 1.2574730336825723 1105 12-13 1.3312839614497065 1105 14-15 1.5041733389781413 1105 16-17 1.4392557090634313 1105 18-19 1.2280182933704538 1105 20-21 1.4266510319264754 1105 22-23 1.2302131371428047 1105 24-25 1.41839422382494 1105 26-27 1.4665937886849676 1105 28-29 1.6279976095909348 1105 30-31 1.483855609090547 1105 32-33 1.3882365802347039 1105 34-35 1.6249494544883802 1105 36-37 1.5023726962485213 1105 38-39 1.340022278627238 1105 40-41 1.4444984583664464 1105 42-43 1.363406224610081 1105 44-45 1.3722656993979356 1105 46-47 1.4836548741901439 1105 48-49 1.5097632872385205 1105 50-51 1.3131023214657702 1105 52-53 1.2238931659114414 1105 54-55 1.2709362259574277 1105 56-57 1.3078461511539103 1105 58-59 1.2794648198506025 1105 60-61 1.276652008653194 1105 62-63 1.3982331286194736 1105 64-65 1.2593615673433973 1105 66-67 1.1758910806999197 1105 68-69 1.042778831317591 1105 70-71 0.8562343819950868 1105 72-73 0.9715653643674358 1105 74-75 0.8336819677805671 1105 76-77 1.0198161035133353 1105 78-79 1.0959207300839857 1105 80-81 1.2140571209736102 1105 82-83 1.185673700653659 1105 84-85 1.0962604881279212 1105 86-87 1.0698502396168728 1105 88-89 1.3615002645504681 1105 90-91 1.3344964720790706 1105 92-93 1.3897344991950007 1105 94-95 1.4933050004504906 1105 96-97 1.1914060143455316 1105 98-99 1.2445328662136461 1105 100-101 1.168146970064921 1105 102-103 1.1587838662976004 1105 104-105 1.1286371035211218 1105 106-107 1.0354308321313788 1105 108-109 1.0812354468758514 1105 110-111 1.089591671887657 1105 112-113 0.9253512679644125 1105 114-115 0.8730791347746845 1105 116-117 0.9507363391117707 1105 118-119 0.965970740346112 1105 120-121 0.8554322487622237 1106 1 0.3985751369663397 1106 2 0.6870983201133924 1106 3 0.4534767568959417 1106 4 0.7912756720347147 1106 5 0.7899494054929832 1106 6 0.7885447637722649 1106 7 0.6148824217504583 1106 8 0.6978498645414106 1106 9 0.8672576871470454 1106 10-11 0.9359223027696828 1106 12-13 0.9205239528798117 1106 14-15 1.0192380701277557 1106 16-17 0.8201417040323662 1106 18-19 0.8512479439975422 1106 20-21 0.9238748064843776 1106 22-23 0.9453088068004476 1106 24-25 1.1115512700888672 1106 26-27 0.9867787305460354 1106 28-29 0.9367332059550222 1106 30-31 1.1274241450024434 1106 32-33 1.1833472472030167 1106 34-35 1.2983605411116983 1106 36-37 1.3812289602850925 1106 38-39 1.2624876057738703 1106 40-41 1.2194852364616864 1106 42-43 1.086553022961425 1106 44-45 0.8719847807414851 1106 46-47 0.9921577200834122 1106 48-49 1.1471576056399684 1106 50-51 0.876099641127233 1106 52-53 0.8435499057867517 1106 54-55 0.8958607524783986 1106 56-57 0.9637114330539021 1106 58-59 1.1419420279836103 1106 60-61 1.0596925373495978 1106 62-63 0.9168145867895632 1106 64-65 0.769582886297691 1106 66-67 0.7902152353594971 1106 68-69 0.8288364263842141 1106 70-71 0.6357918939097829 1106 72-73 0.5428486607193896 1106 74-75 0.5633041284420663 1106 76-77 0.6980728496812283 1106 78-79 0.7390484109843634 1106 80-81 0.6288271497246996 1106 82-83 0.666288815126137 1106 84-85 0.8417066094278489 1106 86-87 0.8105735564646501 1106 88-89 0.5867326030072597 1106 90-91 0.5593789731690322 1106 92-93 0.6874696955309219 1106 94-95 0.6076617416742174 1106 96-97 0.7169778523625894 1106 98-99 0.8865765756583528 1106 100-101 0.7633858857638565 1106 102-103 0.7198053914087339 1106 104-105 0.804529638276378 1106 106-107 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-0.9958480102816125 2316 78-79 -0.767333509046761 2316 80-81 -0.958927950877758 2316 82-83 -1.0680136350237 2316 84-85 -0.9067016107388817 2316 86-87 -0.7470117307957551 2316 88-89 -0.8217289560559848 2316 90-91 -0.8079539128543161 2316 92-93 -0.8608852421228974 2316 94-95 -0.7477868886268979 2316 96-97 -0.7059908575521874 2316 98-99 -0.4988448777420018 2316 100-101 -0.4728078821555606 2316 102-103 -0.49035993943620326 2316 104-105 -0.5645437393556811 2316 106-107 -0.6064867111611072 2316 108-109 -0.2165722495031126 2316 110-111 -0.27605320239032594 2316 112-113 -0.3643836987490836 2316 114-115 -0.3482418261426332 2316 116-117 -0.23599747752204436 2316 118-119 0.03976537387980272 2316 120-121 -0.2520848236269977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25507.0 3 3685.0 4 3132.0 5 3680.0 6 4307.0 7 4616.0 8 5590.0 9 7000.0 10 9401.0 11 11833.0 12 12268.0 13 14253.0 14 18057.0 15 17856.0 16 13454.0 17 13042.0 18 13475.0 19 14695.0 20 16204.0 21 18172.0 22 20096.0 23 22769.0 24 25128.0 25 28623.0 26 32972.0 27 37523.0 28 43899.0 29 51299.0 30 60814.0 31 73062.0 32 88782.0 33 110568.0 34 141783.0 35 188542.0 36 258189.0 37 297301.0 38 110923.0 39 533.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.082912332182865 19.04597079025489 13.920210590599485 21.950906286962763 2 14.170274729533702 21.0170861616486 40.07917437186486 24.73346473695284 3 20.13097032321221 26.114554460598715 30.62337975856811 23.13109545762096 4 11.392436595205725 16.335729880298125 41.35947761623659 30.912355908259563 5 11.02481428867219 37.772670954626534 36.222483776209685 14.980030980491591 6 26.983641865087606 37.36630982733849 19.44240048501358 16.20764782256033 7 25.072568860154103 32.787784518643164 24.140576565406075 17.99907005579665 8 23.456836589036417 32.43002401580316 21.762612724556615 22.350526670603806 9 23.152983370831294 18.746046110036445 24.778153218597883 33.32281730053438 10-11 23.33590771198431 25.41526037428016 29.226715132503944 22.022116781231592 12-13 25.82580679617319 23.72968499093329 28.73866591915532 21.7058422937382 14-15 21.982282587009056 25.637201198871086 29.308059007126424 23.072457206993434 16-17 19.651223105057664 29.036600980831224 29.639863664571003 21.672312249540116 18-19 18.992217048846456 29.76270734071474 30.738462552213274 20.506613058225533 20-21 20.459609164166775 27.55177240407114 29.38153833887573 22.607080092886353 22-23 19.58907980679837 27.335218555814773 30.214228401613834 22.861473235773026 24-25 21.228433066189613 27.14227437296688 29.90455225520482 21.72474030563869 26-27 20.623507769492644 26.006926080570693 32.14906931311248 21.22049683682419 28-29 19.43685037486837 28.55759552748979 31.09918205503827 20.90637204260356 30-31 19.956824610082986 27.503548349322017 30.28312325610431 22.25650378449069 32-33 20.194587729462874 27.31536255923252 30.851403670298726 21.638646041005874 34-35 21.506546863471698 26.372225075338417 30.531431727544167 21.589796333645715 36-37 21.201660269407537 26.36537389702309 31.62671459973315 20.806251233836225 38-39 20.825649798001805 27.609649735989066 31.084771692070206 20.479928773938923 40-41 20.002790812799308 28.301942688111996 30.81237293564989 20.882893563438802 42-43 19.51721480214965 28.465606385605806 29.238697231877975 22.778481580366567 44-45 20.08900780216109 27.50960011537023 29.248540641295733 23.152851441172945 46-47 21.320613663984954 28.065559051278072 29.350174799964684 21.263652484772294 48-49 20.99786258899651 27.453967607234535 29.78757090795957 21.76059889580938 50-51 19.442323229582392 27.94660740678391 31.20491341659325 21.406155947040446 52-53 20.05962887928875 28.71744209767876 29.22797735420169 21.994951668830804 54-55 19.316742226760013 27.89715005193603 29.968168418907815 22.817939302396145 56-57 20.908649736553013 27.136507697327637 29.345961208947724 22.608881357171626 58-59 21.882886906451233 26.912897126289902 29.19144213802015 22.012773829238707 60-61 20.90816269732147 27.43557343032454 30.819631636125678 20.836632236228308 62-63 20.04192437997928 28.143068884546835 30.24003534670388 21.574971388770003 64-65 19.37970977906804 29.40061937123965 29.629811088450285 21.58985976124202 66-67 20.093622866102027 27.647561478932364 28.65780928250981 23.6010063724558 68-69 19.725367930945794 28.312956634579102 29.245959245125885 22.715716189349227 70-71 21.280490641009457 27.415801200909872 28.675654664212573 22.628053493868094 72-73 20.687275004693024 26.360465393153774 30.804636654556166 22.147622947597032 74-75 19.498021230002212 28.861716469795688 29.654359703919635 21.985902596282468 76-77 20.066279101645957 27.70028354296054 29.10401366013005 23.12942369526346 78-79 20.544736148623656 27.6830508150509 29.49750789252806 22.274705143797384 80-81 21.084760965258145 27.717896402248364 28.790494766727804 22.406847865765688 82-83 20.32258439898194 27.046823889664985 30.530562508415933 22.100029202937137 84-85 20.23195605043255 27.46582088047583 29.68534493741352 22.6168781316781 86-87 20.409988616196348 27.960100474320537 29.36605976123088 22.263851148252236 88-89 20.036086687800307 26.935588917465825 28.94433700402957 24.0839873907043 90-91 19.961422146401986 25.614033144275076 29.852196694434596 24.572348014888338 92-93 20.728907497172067 26.623358102841227 30.00715809702614 22.64057630296057 94-95 19.84845124381457 26.28132656940544 30.63833946511585 23.231882721664146 96-97 17.87998357327739 27.132947070895487 31.695710194066272 23.291359161760848 98-99 18.83912482842964 28.046819490669755 29.631901075751344 23.48215460514926 100-101 18.290296938659637 27.180536821667857 30.324690971635587 24.204475268036926 102-103 19.2987892169812 26.23940087202074 30.25653337143509 24.20527653956297 104-105 20.372385433071738 26.19844239037259 29.576834986853797 23.852337189701878 106-107 20.130477470458167 26.10836906484501 31.024033837090887 22.737119627605935 108-109 19.465139485251854 26.214605388918482 30.2661787719273 24.054076353902364 110-111 19.556692199627776 26.993090983392776 29.200880889926967 24.249335927052478 112-113 20.457527832273946 25.603864466759795 28.237023950777612 25.70158375018864 114-115 19.296730379159715 25.88595440668462 29.339333061037998 25.47798215311767 116-117 20.886291571663133 25.682114296730106 28.407967888251466 25.02362624335529 118-119 20.06841656721217 25.69174766356952 29.570597549555757 24.66923821966255 120-121 19.11326167038236 27.9621749435169 28.68444422537637 24.24011916072437 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4652.0 1 5104.5 2 3564.5 3 1521.0 4 1349.5 5 1212.0 6 1255.5 7 1458.0 8 1764.0 9 1853.5 10 1687.0 11 1588.5 12 1681.5 13 1803.5 14 1864.5 15 2036.0 16 2252.0 17 2432.0 18 2676.0 19 2982.5 20 3316.5 21 3709.5 22 4274.5 23 5103.0 24 6208.5 25 7766.5 26 9764.5 27 12477.0 28 16363.0 29 21025.0 30 25773.5 31 30386.5 32 34989.0 33 39065.0 34 42681.0 35 46453.0 36 50395.0 37 54734.5 38 58523.5 39 60346.0 40 61895.5 41 63820.5 42 65264.0 43 67520.0 44 68841.5 45 70179.0 46 75015.5 47 93287.0 48 132813.5 49 129784.0 50 87264.5 51 63813.5 52 50095.5 53 41821.5 54 35940.5 55 30744.0 56 25599.0 57 20348.0 58 16086.0 59 12882.0 60 10107.5 61 7643.5 62 5627.5 63 3921.0 64 2552.0 65 1636.5 66 1023.5 67 608.0 68 365.5 69 216.5 70 133.5 71 83.0 72 61.0 73 48.5 74 34.0 75 28.5 76 28.5 77 29.0 78 20.5 79 12.5 80 10.5 81 7.5 82 4.0 83 3.0 84 4.0 85 4.0 86 4.5 87 5.0 88 3.5 89 2.5 90 2.0 91 1.0 92 2.5 93 3.5 94 3.5 95 3.0 96 1.0 97 1.5 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4776106631092251 2 0.9266425786038981 3 0.9311405772687603 4 0.9075535110993602 5 0.9179757031276998 6 0.9269168468151701 7 0.903604048857042 8 0.8944434906005541 9 0.8923590521948863 10-11 0.8844875545313772 12-13 0.8858040419454831 14-15 0.8867914075060627 16-17 0.9108995832768798 18-19 0.937915002087181 20-21 0.9300435044236719 22-23 0.9065387187176535 24-25 0.8990511965499253 26-27 0.8986946478752715 28-29 0.9214863362319827 30-31 0.9276573709856047 32-33 0.9217606044432548 34-35 0.9333621497800643 36-37 0.9424678543942978 38-39 0.9299063703180359 40-41 0.9384086848674709 42-43 0.9309211626997428 44-45 0.9146570577713075 46-47 0.9072518160669609 48-49 0.9128743143980388 50-51 0.9435923540605134 52-53 0.9156992769741414 54-55 0.9305920408462163 56-57 0.9833063910527127 58-59 0.9452105365070188 60-61 0.9604049954114927 62-63 0.928343041513785 64-65 0.9152330210149788 66-67 0.9184419590868624 68-69 0.9374213193068912 70-71 0.9362968196406757 72-73 0.9413982083703367 74-75 0.9456493656450541 76-77 0.9591982152818956 78-79 1.0222799038744772 80-81 1.0500358468552133 82-83 1.0104040903263956 84-85 0.9704157851229243 86-87 0.9543162411212524 88-89 0.9534111560240545 90-91 0.9650401281819913 92-93 0.9536579974141992 94-95 0.947733804050722 96-97 0.9565652404536835 98-99 0.9882157920344833 100-101 0.9751880519990587 102-103 0.9512718639761321 104-105 0.9428792567112061 106-107 0.9437020613450222 108-109 0.9423307202886618 110-111 0.9397525991027043 112-113 0.9517381199352946 114-115 0.9542065338367436 116-117 0.9784244168920695 118-119 0.9746669423976417 120-121 0.9547002166170333 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1823033.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.34654707571671 #Duplication Level Percentage of deduplicated Percentage of total 1 83.53769121976714 50.41211215790364 2 10.661190459862315 12.86732063938526 3 2.4897319907308124 4.507401863536646 4 1.0145120782121506 2.4488920354685093 5 0.5312157600199254 1.6028518434702532 6 0.3544089244166673 1.2832412904818724 7 0.23177377708321714 0.9790723004768324 8 0.16684958173619022 0.8055036911045317 9 0.1331173328715265 0.7229854255252883 >10 0.7837234764221025 8.77245391370274 >50 0.06266272196484263 2.6292384829941584 >100 0.0272018402001772 2.9358847517729476 >500 0.002220313767388239 0.9661112500016071 >1k 0.0030529314301588296 4.364053759108675 >5k 2.775392209235299E-4 1.0136770855983346 >10k+ 3.7005229456470656E-4 3.68919950946872 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 21351 1.1711801157740973 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 20434 1.1208793258267953 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 12998 0.7129876420229364 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11298 0.6197364501904244 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7217 0.39587873615014096 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5850 0.3208938071883504 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5090 0.27920503907499206 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4662 0.2557276801901008 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4471 0.2452506345195068 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3988 0.21875632531062247 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3767 0.2066336703723959 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3527 0.19346879623133537 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3336 0.18299175056074135 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3124 0.17136277840280456 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3093 0.16966231549291758 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3069 0.16834582807881152 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2973 0.1630798784223873 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2972 0.1630250247801329 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2924 0.1603920499519208 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2643 0.1449781764784291 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2642 0.14492332283617468 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2494 0.13680498378252068 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2442 0.1339525943852909 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2395 0.1313744731993332 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2320 0.12726045003025177 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2105 0.11546691694555172 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1900 0.10422192028339587 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 5.485364225441887E-5 0.0 6 0.0 0.0 0.0 5.485364225441887E-5 0.0 7 0.0 0.0 0.0 5.485364225441887E-5 0.0 8 0.0 0.0 0.0 1.0970728450883774E-4 0.0 9 0.0 0.0 0.0 1.0970728450883774E-4 0.0 10-11 0.0 0.0 0.0 1.6456092676325661E-4 0.0 12-13 0.0 0.0 0.0 2.1941456901767548E-4 0.0 14-15 0.0 0.0 0.0 3.839754957809321E-4 0.0 16-17 0.0 0.0 0.0 4.114023169081415E-4 0.0 18-19 0.0 0.0 0.0 6.30816885925817E-4 0.0 20-21 0.0 0.0 0.0 0.0011519264873427963 0.0 22-23 0.0 0.0 0.0 0.002605548007084896 0.0 24-25 0.0 0.0 0.0 0.004497998664862347 0.0 26-27 0.0 0.0 0.0 0.006417876143767008 0.0 28-29 0.0 0.0 0.0 0.013027740035424482 0.0 30-31 0.0 0.0 0.0 0.024684139014488495 0.0 32-33 0.0 0.0 0.0 0.04470571843735138 0.0 34-35 0.0 0.0 0.0 0.06555010249403055 0.0 36-37 0.0 0.0 0.0 0.08710758390001716 0.0 38-39 0.0 2.7426821127209435E-5 0.0 0.10304256697492585 0.0 40-41 0.0 5.485364225441887E-5 0.0 0.12915290068802923 0.0 42-43 0.0 5.485364225441887E-5 0.0 0.1654660118604545 0.0 44-45 0.0 5.485364225441887E-5 0.0 0.22692951800653088 0.0 46-47 0.0 5.485364225441887E-5 0.0 0.3674919762834792 0.0 48-49 0.0 5.485364225441887E-5 0.0 0.7303762466175873 0.0 50-51 0.0 5.485364225441887E-5 0.0 0.9107898759923709 0.0 52-53 0.0 5.485364225441887E-5 0.0 1.0626796113948567 0.0 54-55 0.0 5.485364225441887E-5 0.0 1.2611400890713442 0.0 56-57 0.0 5.485364225441887E-5 0.0 1.3805838950803415 0.0 58-59 0.0 5.485364225441887E-5 0.0 1.5662909009326764 0.0 60-61 0.0 5.485364225441887E-5 0.0 1.9079742385354517 0.0 62-63 0.0 5.485364225441887E-5 0.0 2.0728642871522345 0.0 64-65 0.0 5.485364225441887E-5 0.0 2.2573645128749726 0.0 66-67 0.0 5.485364225441887E-5 0.0 2.788018648044221 0.0 68-69 0.0 5.485364225441887E-5 0.0 3.2842795495199484 0.0 70-71 0.0 5.485364225441887E-5 0.0 3.575634670354294 0.0 72-73 0.0 5.485364225441887E-5 0.0 3.871295802105612 0.0 74-75 0.0 5.485364225441887E-5 0.0 4.075159363544159 0.0 76-77 0.0 5.485364225441887E-5 0.0 4.183824428850164 0.0 78-79 0.0 5.485364225441887E-5 0.0 4.36451232643622 0.0 80-81 0.0 5.485364225441887E-5 0.0 4.717660075270167 0.0 82-83 0.0 5.485364225441887E-5 0.0 5.168365026853601 0.0 84-85 0.0 5.485364225441887E-5 0.0 5.7946564872934285 0.0 86-87 0.0 5.485364225441887E-5 0.0 7.113968863975583 0.0 88-89 0.0 5.485364225441887E-5 0.0 8.652311834179635 0.0 90-91 0.0 5.485364225441887E-5 0.0 10.031140412707835 0.0 92-93 0.0 5.485364225441887E-5 0.0 11.33854954902078 0.0 94-95 0.0 5.485364225441887E-5 0.0 12.460498520871536 0.0 96-97 0.0 5.485364225441887E-5 0.0 13.553512196433086 0.0 98-99 0.0 5.485364225441887E-5 0.0 14.654973332901818 0.0 100-101 0.0 5.485364225441887E-5 0.0 15.572208511859083 0.0 102-103 0.0 5.485364225441887E-5 0.0 16.336292321642013 0.0 104-105 0.0 5.485364225441887E-5 0.0 17.247356465845655 0.0 106-107 0.0 5.485364225441887E-5 0.0 18.355509746669426 0.0 108-109 0.0 5.485364225441887E-5 0.0 19.52375519258291 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 305 0.0 64.12973 2 TACCTGG 2630 0.0 60.152992 2 ACCTGGG 2795 0.0 55.297962 3 ATAACGC 455 0.0 45.460033 3 TAACGCA 430 0.0 45.428146 4 GTACCTG 3785 0.0 42.044796 1 GTACCCG 295 0.0 41.045574 1 GTATAAC 745 0.0 36.37564 1 TCGAGCG 70 0.0033199438 32.81316 9 GTACACG 300 0.0 32.67358 1 CCTGGGG 2005 0.0 32.380135 4 TACCCGG 350 0.0 31.229559 2 GAGTACG 505 0.0 29.685955 1 TACACGG 285 0.0 28.259434 2 TCACGCA 350 0.0 27.905863 4 AGTACGC 490 0.0 24.654915 2 GTACGCA 470 0.0 24.449545 3 CTGGGGG 1370 0.0 23.905231 5 TAGTACG 315 2.5465852E-11 23.710863 4 TATCACG 365 0.0 23.6417 2 >>END_MODULE