##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003857_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1823033 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.65426901213527 31.0 31.0 34.0 30.0 34.0 2 31.868596454370273 31.0 31.0 34.0 30.0 34.0 3 31.90667804696898 31.0 31.0 34.0 30.0 34.0 4 35.65096408018944 37.0 35.0 37.0 33.0 37.0 5 35.53289984328315 37.0 35.0 37.0 33.0 37.0 6 35.57926488439869 37.0 35.0 37.0 33.0 37.0 7 35.513202997422425 37.0 35.0 37.0 33.0 37.0 8 35.510889270792134 37.0 35.0 37.0 33.0 37.0 9 37.07253461676228 39.0 37.0 39.0 33.0 39.0 10-11 36.98286262508688 39.0 37.0 39.0 33.0 39.0 12-13 36.94263049544358 39.0 37.0 39.0 33.0 39.0 14-15 38.00965616091426 40.0 37.0 41.0 33.0 41.0 16-17 38.04174279895098 40.0 37.0 41.0 33.0 41.0 18-19 38.07850735559916 40.0 37.0 41.0 33.0 41.0 20-21 38.01235578291781 40.0 37.0 41.0 33.0 41.0 22-23 37.920765010836334 40.0 37.0 41.0 33.0 41.0 24-25 37.80929692441113 39.5 37.0 41.0 32.5 41.0 26-27 37.5592353511977 39.0 36.5 41.0 32.0 41.0 28-29 37.389914225359604 39.0 36.0 41.0 32.0 41.0 30-31 37.167556209898564 39.0 36.0 40.0 31.0 41.0 32-33 36.9003402571429 39.0 35.5 40.0 31.0 41.0 34-35 36.84728608862264 39.0 35.0 40.0 31.0 41.0 36-37 36.63930027596867 39.0 35.0 40.0 30.0 41.0 38-39 36.385912377888936 38.0 35.0 40.0 30.0 41.0 40-41 36.53377174192678 39.0 35.0 40.0 30.0 41.0 42-43 36.390588376622915 39.0 35.0 40.5 30.0 41.0 44-45 36.22150312144652 39.0 35.0 41.0 30.0 41.0 46-47 35.91349498336015 39.0 35.0 40.0 29.0 41.0 48-49 35.616784775700715 39.0 35.0 40.0 28.0 41.0 50-51 35.33944942302196 38.5 35.0 40.0 27.0 41.0 52-53 35.168318401257686 38.0 34.0 40.0 26.5 41.0 54-55 34.96342249427191 38.0 34.0 40.0 26.0 41.0 56-57 34.696617121028524 38.0 34.0 40.0 25.5 41.0 58-59 34.43760370766739 38.0 34.0 40.0 25.0 41.0 60-61 33.95105546635744 37.5 33.0 40.0 23.0 41.0 62-63 33.74859698096524 37.0 33.0 40.0 23.0 41.0 64-65 33.312260666702144 36.5 32.0 39.5 22.0 41.0 66-67 32.94832786899634 36.0 32.0 39.0 22.0 41.0 68-69 32.642515247941205 36.0 32.0 39.0 21.0 40.0 70-71 32.17506238230466 35.0 31.0 38.5 20.0 40.0 72-73 31.778697094347717 35.0 31.0 37.5 19.0 40.0 74-75 31.408983271284722 35.0 31.0 37.0 18.0 39.0 76-77 30.432762325202013 34.0 29.5 36.0 17.5 39.0 78-79 30.80241388938105 34.0 30.5 36.0 17.0 39.0 80-81 30.584163588920223 34.0 30.5 36.0 13.0 37.5 82-83 30.255694219468324 34.0 30.0 35.0 9.5 37.0 84-85 29.9204356147146 34.0 30.0 35.0 7.0 37.0 86-87 29.61790049878417 34.0 30.0 35.0 4.5 36.0 88-89 29.292340017981026 34.0 29.0 35.0 2.0 36.0 90-91 28.972836750623824 34.0 29.0 35.0 2.0 36.0 92-93 28.713282480350053 34.0 29.0 35.0 2.0 35.0 94-95 28.384439831862615 34.0 29.0 35.0 2.0 35.0 96-97 27.972142029244672 34.0 29.0 35.0 2.0 35.0 98-99 27.620601217860568 34.0 29.0 35.0 2.0 35.0 100-101 26.631359936984136 33.0 26.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.007967175987410258 1101 2 0.10993691277382922 1101 3 0.06523257117503789 1101 4 0.12669826923696803 1101 5 0.05143217710525505 1101 6 0.1385813527101618 1101 7 0.08782932258461784 1101 8 0.029019380877016943 1101 9 0.1974586148570907 1101 10-11 0.15612350886974014 1101 12-13 0.0404799126829829 1101 14-15 0.07224765362676777 1101 16-17 0.22320649948778026 1101 18-19 0.06160695314766684 1101 20-21 0.06091561118209654 1101 22-23 -0.05490779383587352 1101 24-25 0.037206724941690084 1101 26-27 0.06605561390915682 1101 28-29 -0.036153611895336724 1101 30-31 0.10693137811362163 1101 32-33 0.09405492512659919 1101 34-35 -0.028588727260945745 1101 36-37 -0.005311803495700929 1101 38-39 -0.02511563775549064 1101 40-41 0.12934874265511098 1101 42-43 0.04194739768665556 1101 44-45 0.025212360789929278 1101 46-47 0.19808249592686877 1101 48-49 0.12772103376361343 1101 50-51 0.01575035844386008 1101 52-53 0.03543804228616665 1101 54-55 0.25889666735731254 1101 56-57 0.17471375998481165 1101 58-59 -0.006307232083109682 1101 60-61 0.034716691256981846 1101 62-63 -0.017173243568322505 1101 64-65 -0.14106762794229866 1101 66-67 -0.059713228051712974 1101 68-69 -0.09776223525025785 1101 70-71 0.0031360427394844237 1101 72-73 -0.11325223847351751 1101 74-75 -0.14362023688637038 1101 76-77 -0.055376867559495224 1101 78-79 -0.07321173108872614 1101 80-81 -0.15431388608807595 1101 82-83 -0.15050456396328826 1101 84-85 -0.1981297327720739 1101 86-87 -0.18412458376608143 1101 88-89 -0.19087681255610178 1101 90-91 -0.18495123382562184 1101 92-93 0.012537358239242735 1101 94-95 -0.09521850498511597 1101 96-97 -0.07749831356023407 1101 98-99 -0.19731023469663 1101 100-101 0.1481802904709788 1102 1 -0.005971770251779418 1102 2 -0.07109882739009876 1102 3 -0.059529819783584514 1102 4 -0.008737708065481797 1102 5 -0.24437696402040388 1102 6 -0.08021364009099585 1102 7 -0.08875830574779542 1102 8 -0.007944116728069162 1102 9 -0.05453341935861289 1102 10-11 -0.06769126900109512 1102 12-13 0.002050179087419224 1102 14-15 0.058627773983985776 1102 16-17 -0.09955744651615106 1102 18-19 -0.1738121445025982 1102 20-21 -0.1100714426539966 1102 22-23 -0.10224521984770973 1102 24-25 -0.13468636039995374 1102 26-27 -0.1195693919722558 1102 28-29 -0.07834068549296802 1102 30-31 -0.09299369269857749 1102 32-33 -0.0029366150505012456 1102 34-35 -0.09204063377943328 1102 36-37 -0.2520008626580008 1102 38-39 -0.325430822620028 1102 40-41 -0.09643983705448989 1102 42-43 -0.15607827114750705 1102 44-45 -0.06466443539327571 1102 46-47 -0.07883555012693932 1102 48-49 -0.27050554410157446 1102 50-51 -0.21212671063684496 1102 52-53 -0.4124582354858646 1102 54-55 -0.360578922430669 1102 56-57 -0.4808795803748467 1102 58-59 -0.6002786672417244 1102 60-61 -0.5054495292065653 1102 62-63 -0.682353285349869 1102 64-65 -0.5044080228908712 1102 66-67 -0.626089078503874 1102 68-69 -0.6837352442746933 1102 70-71 -0.7378498112885836 1102 72-73 -0.6496428865580555 1102 74-75 -0.6091763361457865 1102 76-77 -0.7199278923367096 1102 78-79 -0.6922109265115743 1102 80-81 -0.6839547317880594 1102 82-83 -0.7850862661850506 1102 84-85 -0.9342078426274405 1102 86-87 -0.8696831296928487 1102 88-89 -0.8868278133371525 1102 90-91 -0.8067655670617171 1102 92-93 -0.656638514797546 1102 94-95 -0.6755877047366852 1102 96-97 -0.5317162269293902 1102 98-99 -0.9032069970970014 1102 100-101 -0.9523662691383059 1103 1 -0.14816559230615312 1103 2 -0.19274602286461118 1103 3 -0.18167275255904158 1103 4 -0.16761621873587274 1103 5 -0.12802052087938165 1103 6 -0.09800788183753184 1103 7 -0.09205166992700242 1103 8 -0.051111124047018563 1103 9 -0.09251363478774977 1103 10-11 -0.13057979709447665 1103 12-13 -0.1599598190836531 1103 14-15 -0.09195122793158106 1103 16-17 -0.22476624978384763 1103 18-19 -0.1935525963454907 1103 20-21 -0.07391454580906753 1103 22-23 -0.0650413386578137 1103 24-25 -0.13221762072780763 1103 26-27 -0.2994957212380811 1103 28-29 -0.24528639824769982 1103 30-31 -0.10978234175828305 1103 32-33 -0.1163601864767827 1103 34-35 -0.4685755744353486 1103 36-37 -0.4412730432924832 1103 38-39 -0.3484763502353232 1103 40-41 -0.354197885165064 1103 42-43 -0.45408553554394615 1103 44-45 -0.3097527857271345 1103 46-47 -0.1546251200708184 1103 48-49 -0.22355376698763507 1103 50-51 -0.2657107790197202 1103 52-53 -0.34698096399829836 1103 54-55 -0.6490410779413764 1103 56-57 -0.5100852455773008 1103 58-59 -0.4972058981397893 1103 60-61 -0.3908170967567983 1103 62-63 -0.6231046562480103 1103 64-65 -0.7290294817382659 1103 66-67 -0.8699119350416709 1103 68-69 -0.846111510490438 1103 70-71 -0.8212203115500856 1103 72-73 -0.7448437002731367 1103 74-75 -0.6773865800998706 1103 76-77 -0.6305952285428553 1103 78-79 -0.515910859450674 1103 80-81 -0.6461415268865132 1103 82-83 -0.6224326116866763 1103 84-85 -0.6184835046533195 1103 86-87 -0.5762148296065348 1103 88-89 -0.5479745227003328 1103 90-91 -0.727424175724444 1103 92-93 -0.7897624298708443 1103 94-95 -1.0272847627202992 1103 96-97 -0.816207350183479 1103 98-99 -0.7103927814689257 1103 100-101 -0.8197552576737799 1104 1 0.02661424445888727 1104 2 0.028239308358926962 1104 3 -0.012837405380452083 1104 4 0.008852221518225178 1104 5 -0.030341404887821 1104 6 -0.06447957889555056 1104 7 0.0234359130968258 1104 8 0.07306225635097263 1104 9 -0.054703128140914714 1104 10-11 -0.11911913698309462 1104 12-13 -0.04615804330308748 1104 14-15 0.004856446282751392 1104 16-17 -0.06267149981756148 1104 18-19 -0.031050416918624535 1104 20-21 -0.0700609901358149 1104 22-23 0.004101760043077718 1104 24-25 -0.004529688337747473 1104 26-27 -0.008066543564623885 1104 28-29 0.11023437785853929 1104 30-31 -0.011268628639939493 1104 32-33 0.11458093116621626 1104 34-35 0.04351489891971738 1104 36-37 0.04325384836779733 1104 38-39 0.0837997280463938 1104 40-41 0.004843199909885243 1104 42-43 0.004404524624682438 1104 44-45 0.11740994590893195 1104 46-47 -0.022783117827238186 1104 48-49 0.04369676676634526 1104 50-51 0.14362243149811604 1104 52-53 0.12852836394869627 1104 54-55 0.09714944837787698 1104 56-57 -0.009552971966293455 1104 58-59 0.059461009771602846 1104 60-61 0.07479154726137693 1104 62-63 0.13451840323965314 1104 64-65 0.04661357255167786 1104 66-67 -0.07562450071232263 1104 68-69 -0.07383562959077494 1104 70-71 -0.014454877095730012 1104 72-73 0.05335453052570216 1104 74-75 -0.0151602420962611 1104 76-77 -0.1685717006017846 1104 78-79 -0.10929082977757076 1104 80-81 0.022484430827667268 1104 82-83 -0.10703121882730215 1104 84-85 -0.13580098382128725 1104 86-87 -0.2994947272310391 1104 88-89 -0.2502080651489855 1104 90-91 -0.24596032460315342 1104 92-93 -0.31001218396569996 1104 94-95 -0.18946718321645406 1104 96-97 -0.17105324414325906 1104 98-99 -0.31881737658898146 1104 100-101 -0.284282576995178 1105 1 0.07201042583975692 1105 2 0.029950849297883053 1105 3 0.14419541401871427 1105 4 0.05046574170067686 1105 5 0.006034856648092557 1105 6 0.1249628041810027 1105 7 -0.013534928417882952 1105 8 0.09051105522863168 1105 9 0.011371695502418788 1105 10-11 0.07197462120122822 1105 12-13 0.07367176525632146 1105 14-15 0.11347275957543701 1105 16-17 0.09128259860806764 1105 18-19 0.1296475633498133 1105 20-21 0.16389596793639072 1105 22-23 0.20273818995837445 1105 24-25 0.18204101500789704 1105 26-27 0.2578129888690057 1105 28-29 0.15052424975487355 1105 30-31 0.20590595561417047 1105 32-33 0.19022212196834687 1105 34-35 0.2518472294892007 1105 36-37 0.14804856963012014 1105 38-39 0.20240038406630845 1105 40-41 0.2931093763783963 1105 42-43 0.2948522433487142 1105 44-45 0.1737963011478243 1105 46-47 0.16252622656339355 1105 48-49 0.18801689995225956 1105 50-51 0.2616607139547469 1105 52-53 0.38780770470106773 1105 54-55 0.2957306672951461 1105 56-57 0.290479517582547 1105 58-59 0.2856325092881562 1105 60-61 0.17650754755018028 1105 62-63 -0.03188754482520295 1105 64-65 0.1698473514896719 1105 66-67 0.09932181013125785 1105 68-69 0.16343717361316124 1105 70-71 0.3839590100275103 1105 72-73 0.11669393234993208 1105 74-75 0.07170683105547937 1105 76-77 0.1370647052471874 1105 78-79 0.19038738920056986 1105 80-81 0.352380761561367 1105 82-83 0.2579609029879819 1105 84-85 0.21699800142888392 1105 86-87 0.2723691111224831 1105 88-89 0.411690046493316 1105 90-91 0.45729294806415055 1105 92-93 0.26432022304977565 1105 94-95 0.3183423416716735 1105 96-97 0.25604700345683185 1105 98-99 0.25236835745539565 1105 100-101 0.6229264928976974 1106 1 -0.006978656537580008 1106 2 -0.002853707727105359 1106 3 0.058101711540782475 1106 4 0.1267123088746942 1106 5 0.07925873907876735 1106 6 -0.02364091772900423 1106 7 0.03305112333407578 1106 8 0.06992833255003461 1106 9 -0.02655082488478655 1106 10-11 -0.13979759347156318 1106 12-13 -0.18638212617874217 1106 14-15 -0.014438494100851074 1106 16-17 -0.04513150096148166 1106 18-19 0.003045390612562926 1106 20-21 -0.037143281269173656 1106 22-23 -0.23924536543803043 1106 24-25 -0.09985523836125054 1106 26-27 -0.08892603245534048 1106 28-29 -0.05180685071213276 1106 30-31 -0.0899407182340255 1106 32-33 -0.17597070359390443 1106 34-35 -0.13350087741869743 1106 36-37 0.006790683307045242 1106 38-39 -0.022642427936936826 1106 40-41 -0.13133286899319074 1106 42-43 -0.20240898703005428 1106 44-45 -0.035222536133609594 1106 46-47 -0.0907919846065326 1106 48-49 0.02290077941643176 1106 50-51 -0.014235116912743706 1106 52-53 -0.12090002453229687 1106 54-55 -0.01173330395076988 1106 56-57 -0.12515311872293466 1106 58-59 -0.13508325646613173 1106 60-61 -0.0913161793639361 1106 62-63 -0.24022543528339924 1106 64-65 -0.2894082065795871 1106 66-67 -0.3219730226135198 1106 68-69 -0.2078664987053358 1106 70-71 -0.20778303023600486 1106 72-73 -0.16026077457325272 1106 74-75 -0.23677706219243433 1106 76-77 -0.1467650175947206 1106 78-79 -0.15718727148714606 1106 80-81 -0.15912341925735873 1106 82-83 -0.01862673689078065 1106 84-85 -0.05135872181604739 1106 86-87 0.05077897668605047 1106 88-89 0.04279253610681266 1106 90-91 0.02739413870978069 1106 92-93 0.04433394686254033 1106 94-95 0.0427525248606031 1106 96-97 0.22803866429287467 1106 98-99 0.19945152286179635 1106 100-101 0.16425599475025976 1107 1 -0.047280196273391084 1107 2 -0.05261206179992328 1107 3 -0.030092214687858387 1107 4 -0.007375199378770958 1107 5 0.051579438582194825 1107 6 0.07373867930639477 1107 7 -0.08388870546261984 1107 8 0.06783966783424233 1107 9 0.0576517435555175 1107 10-11 0.04471479747521556 1107 12-13 0.04649500360680747 1107 14-15 0.019389396175888862 1107 16-17 -0.12602662623839223 1107 18-19 -0.12742797754487611 1107 20-21 -0.08014180052857256 1107 22-23 -0.11424116544818474 1107 24-25 -0.26894819683409565 1107 26-27 -0.1651486201960637 1107 28-29 -0.23140290936351704 1107 30-31 -0.08147178057750892 1107 32-33 -0.06804483439135822 1107 34-35 -0.3018649911427076 1107 36-37 -0.22097066350272598 1107 38-39 0.06314988549561207 1107 40-41 -0.07125719103669326 1107 42-43 -0.20884649704165525 1107 44-45 -0.1909272944290734 1107 46-47 -0.30005579475917443 1107 48-49 -0.3251439788008952 1107 50-51 -0.39773521263344236 1107 52-53 -0.22735023218761086 1107 54-55 -0.4591931891640755 1107 56-57 -0.4897911577379048 1107 58-59 -0.5072566633025488 1107 60-61 -0.47717315310520547 1107 62-63 -0.4380928171855487 1107 64-65 -0.5683996620421112 1107 66-67 -0.47317988481088236 1107 68-69 -0.5998368995211081 1107 70-71 -0.5886965182591979 1107 72-73 -0.681467402352574 1107 74-75 -0.7562347360622468 1107 76-77 -0.7637040649979561 1107 78-79 -0.7723744347554025 1107 80-81 -0.6088811872850819 1107 82-83 -0.593911066435659 1107 84-85 -0.6093172141955101 1107 86-87 -0.4486007922559061 1107 88-89 -0.6473867155526669 1107 90-91 -0.400113255225822 1107 92-93 -0.5212230108601652 1107 94-95 -0.3835843416734903 1107 96-97 -0.1446948063052389 1107 98-99 0.009034336894060857 1107 100-101 0.06272016590668628 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-0.2919845202493363 2316 74-75 -0.3333100339188704 2316 76-77 -0.2721759131638173 2316 78-79 -0.11810074237769541 2316 80-81 -0.2901525893187902 2316 82-83 -0.4024183515200761 2316 84-85 -0.4515283660072704 2316 86-87 -0.12504027718961908 2316 88-89 -0.1521136274676813 2316 90-91 -0.3892744074106034 2316 92-93 -0.4564825583120644 2316 94-95 -0.537814017668147 2316 96-97 -0.27472871384099307 2316 98-99 -0.5492150047252906 2316 100-101 -0.9975082343589037 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 21.0 5 34.0 6 117.0 7 294.0 8 658.0 9 1323.0 10 2188.0 11 3672.0 12 6046.0 13 8090.0 14 9288.0 15 11627.0 16 14444.0 17 18474.0 18 16745.0 19 11569.0 20 10393.0 21 10509.0 22 11102.0 23 12251.0 24 14298.0 25 17461.0 26 21257.0 27 26572.0 28 33277.0 29 41835.0 30 51845.0 31 63607.0 32 79199.0 33 99415.0 34 128581.0 35 171691.0 36 242041.0 37 325420.0 38 295868.0 39 61773.0 40 43.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.57049624403458 20.317665550048076 14.070656492705394 20.04118171321195 2 15.337995772755722 23.390166527971072 38.645493508552406 22.626344190720797 3 21.995991508847876 27.11159235445072 29.471520175267212 21.420895961434187 4 12.707888447438966 17.456129428265974 39.781945801310236 30.054036322984828 5 11.930008946629052 39.60668841430737 34.040963602962755 14.422339036100828 6 27.741238656111218 39.30262511190297 18.00633897382787 14.949797258157945 7 27.199507633707125 33.23406652540025 22.432835829082634 17.13359001180999 8 25.212105321187273 33.11953212037302 20.014942132150104 21.653420426289596 9 24.53921569165232 20.169519696023055 22.926408902087893 32.36485571023673 10-11 24.566435146838277 26.597865307461333 28.183223936466074 20.652475609234315 12-13 27.556879112994665 24.363053219552253 27.624266812504217 20.45580085494887 14-15 22.545712165171043 27.014760971440765 28.39812548992503 22.041401373463167 16-17 20.218284583987234 29.52623457721281 28.91705745315636 21.338423385643594 18-19 19.80359104854383 30.618562582246177 30.08478178946843 19.493064579741564 20-21 21.868438210867634 27.575046637991264 29.322424939646968 21.234090211494134 22-23 20.58561199934395 28.148503071529696 29.55996956719928 21.705915361927076 24-25 22.17189705287836 28.328450444945318 29.556212092704847 19.94344040947147 26-27 22.03088112388354 26.56869422157685 31.628040804358566 19.772383850181043 28-29 20.49745424861521 29.748562820344727 30.33487287152308 19.419110059516985 30-31 21.549393323888378 28.00518969686081 29.681993918838295 20.763423060412514 32-33 21.31933526357192 28.472207746042333 30.02614489516259 20.182312095223153 34-35 22.522012490636413 27.033102188809995 30.428899675816172 20.015985644737423 36-37 22.434518256102535 26.95476869165993 31.08473885347523 19.525974198762306 38-39 21.44081104767182 28.373557015131713 30.955178150428388 19.230453786768084 40-41 20.8713389270545 28.748934307242102 29.9994294360524 20.380297329651 42-43 20.34609357044003 29.39088321495003 28.74958928335362 21.51343393125632 44-45 21.62637758065817 27.681232319985433 28.815962190481464 21.876427908874934 46-47 22.6168609812831 29.022098630769683 28.417446434688593 19.943593953258624 48-49 22.464431527021176 28.46092199098974 28.881347732048734 20.193298749940347 50-51 20.648474273367516 28.889383790639005 30.32536986439631 20.136772071597168 52-53 21.580355374806707 29.84375488540251 28.12727471197724 20.44861502781354 54-55 20.534625538868468 29.102106215301642 28.593338683391906 21.76992956243798 56-57 22.067215850730182 28.183016251990185 28.66263578180562 21.087132115474017 58-59 23.54885096483628 27.667297759032422 28.028482259886633 20.755369016244664 60-61 21.623755206497545 28.9559026630112 29.745137059290638 19.67520507120061 62-63 20.975380138647193 28.76811268211434 29.476133942228145 20.78037323701033 64-65 20.4665858155717 30.632531773382794 28.420303136729814 20.48057927431569 66-67 21.369014013412627 28.08111549858966 27.94389477513304 22.605975712864677 68-69 21.15282291065555 29.215194414518393 27.818302896525093 21.813679778300965 70-71 22.493977053561437 28.933702609065797 27.494723065142168 21.077597272230598 72-73 22.05053336939046 27.247778838891012 29.44090973668606 21.260778055032464 74-75 20.729526215248494 30.578747224747776 28.18040819349079 20.51131836651294 76-77 21.668532604730686 28.618927907503593 27.635621516450882 22.076917971314835 78-79 21.525364598446657 29.30415960654579 27.81038522067346 21.36009057433409 80-81 22.36613380010126 28.86656467546117 27.555919174255212 21.21138235018236 82-83 21.869906323666747 28.149936986861178 29.020656515997384 20.95950017347469 84-85 20.944234918595754 28.86381831581078 28.277706666066933 21.91424009952653 86-87 21.222800292152503 28.754202160644933 27.455149297553156 22.567848249649415 88-89 21.03192865954703 28.716759378464353 27.489683401233002 22.76162856075562 90-91 21.38674944446974 26.681113287581738 28.115426325250283 23.81671094269824 92-93 21.393249601076885 28.170938211211755 28.25025657791165 22.185555609799714 94-95 21.11404029882103 27.988032019203185 28.712622001490924 22.185305680484863 96-97 19.166293889536014 28.841156350545262 30.164064906280952 21.82848485363778 98-99 20.47751990031944 29.456285121941654 27.708501837743725 22.357693139995177 100-101 19.515214780362296 29.175071934464558 27.28855271779392 24.021160567379233 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 468.0 1 326.5 2 542.0 3 829.5 4 592.0 5 465.5 6 633.0 7 958.5 8 1358.5 9 1703.0 10 1772.5 11 1667.0 12 1727.5 13 1927.5 14 2152.0 15 2318.5 16 2507.0 17 2756.0 18 2993.5 19 3299.0 20 3623.5 21 4015.0 22 4610.5 23 5308.5 24 6216.5 25 7685.5 26 9663.0 27 12299.0 28 15928.0 29 20537.5 30 25433.0 31 30196.0 32 34533.5 33 38366.5 34 41971.0 35 45652.0 36 49101.0 37 52776.0 38 56899.0 39 59469.0 40 60942.5 41 62018.5 42 63925.5 43 67020.5 44 67966.0 45 68719.0 46 73157.5 47 93219.5 48 139019.5 49 136287.5 50 90607.0 51 67601.5 52 52495.0 53 42952.0 54 36566.0 55 31345.5 56 26697.0 57 21677.0 58 17267.5 59 13737.5 60 10656.5 61 8016.5 62 5760.5 63 3978.0 64 2564.0 65 1606.0 66 974.0 67 540.0 68 288.5 69 158.0 70 88.0 71 51.0 72 26.5 73 17.0 74 14.0 75 9.0 76 5.0 77 4.0 78 2.5 79 0.5 80 2.0 81 1.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04744840055007232 2 0.005265949656424212 3 0.049423131671231406 4 0.0 5 0.0 6 0.0013713410563604717 7 0.0 8 0.0 9 0.0 10-11 3.8397549578093215E-4 12-13 0.0 14-15 0.0027426821127209434 16-17 0.0 18-19 0.0 20-21 0.011875813548081687 22-23 0.0 24-25 0.0 26-27 0.0076520830944914325 28-29 0.010531899312848423 30-31 0.010449618849466795 32-33 0.006308168859258171 34-35 0.008090912232526785 36-37 0.020213567170753354 38-39 0.011272423483283079 40-41 0.015084751619965189 42-43 0.0 44-45 0.0 46-47 8.228046338162831E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0013987678774876812 58-59 1.9198774789046607E-4 60-61 0.010449618849466795 62-63 0.015194458904474028 64-65 0.041304792617577406 66-67 0.023148237031364764 68-69 0.0011519264873427963 70-71 2.7426821127209437E-4 72-73 0.0 74-75 2.7426821127209435E-5 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.7426821127209435E-5 84-85 8.228046338162831E-5 86-87 6.856705281802359E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.0970728450883774E-4 96-97 2.4684139014488496E-4 98-99 5.211096014169793E-4 100-101 0.0010696460239611682 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1823033.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.85966594960596 #Duplication Level Percentage of deduplicated Percentage of total 1 81.79035708539782 46.50572381774706 2 11.744212943587101 13.355440496268024 3 2.786350616588634 4.752928958331251 4 1.1358189398464476 2.5832914199561836 5 0.5778700941001673 1.642875025640144 6 0.3838258982606496 1.309452741474479 7 0.2433857118225222 0.9687181189794971 8 0.19058807926470803 0.8669419616774656 9 0.1335799923224676 0.6835782366905793 >10 0.8953968242115347 9.617635881342148 >50 0.07594783865693566 2.9647053990473933 >100 0.03604251570639092 3.772697000256501 >500 0.0028247109824164245 1.119964947780523 >1k 0.002629903328456671 3.3975002334880906 >5k 8.766344428188903E-4 3.2423156927067125 >10k+ 2.922114809396301E-4 3.2162300686139114 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 37140 2.037264273329117 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10552 0.5788156330686279 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10380 0.5693808066008679 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7645 0.41935609503503224 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 7258 0.3981277354825722 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7078 0.3882540798767768 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6952 0.38134252095272 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6766 0.37113974349339807 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6557 0.35967533226222453 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5561 0.3050411045768234 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5519 0.30273725160213777 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5207 0.28562291521875904 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4862 0.2666984086409846 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4311 0.2364740517587998 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4224 0.23170178488266532 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4122 0.2261067133727146 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3897 0.21376464386547034 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3344 0.1834305796987767 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3211 0.176135045278939 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 2900 0.15907556253781474 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2481 0.1360918864332132 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2309 0.12665705996545318 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2070 0.11354703946664707 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1917 0.10515443220172098 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1881 0.1031797010805619 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 5.485364225441887E-5 0.0 9 0.0 0.0 0.0 5.485364225441887E-5 0.0 10-11 0.0 0.0 0.0 1.0970728450883774E-4 0.0 12-13 5.485364225441887E-5 0.0 0.0 1.6456092676325661E-4 0.0 14-15 1.6456092676325661E-4 0.0 0.0 3.2912185352651323E-4 0.0 16-17 1.6456092676325661E-4 0.0 0.0 3.565486746537227E-4 0.0 18-19 1.6456092676325661E-4 0.0 0.0 5.211096014169792E-4 0.0 20-21 1.6456092676325661E-4 0.0 0.0 0.0010147923817067491 0.0 22-23 1.6456092676325661E-4 0.0 0.0 0.0024135602591944306 0.0 24-25 1.6456092676325661E-4 0.0 0.0 0.004278584095844673 0.0 26-27 1.6456092676325661E-4 0.0 0.0 0.006335595680385379 0.0 28-29 1.6456092676325661E-4 0.0 0.0 0.01316487414106053 0.0 30-31 1.6456092676325661E-4 0.0 0.0 0.025122968152523843 0.0 32-33 1.9198774789046605E-4 0.0 0.0 0.04615933995709348 0.0 34-35 2.1941456901767548E-4 0.0 0.0 0.06774424818420731 0.0 36-37 2.1941456901767548E-4 0.0 0.0 0.0895485709803388 0.0 38-39 2.7426821127209437E-4 0.0 0.0 0.10627893186793656 0.0 40-41 2.7426821127209437E-4 0.0 0.0 0.13367832617401879 0.0 42-43 2.7426821127209437E-4 0.0 0.0 0.17108851019153248 0.0 44-45 2.7426821127209437E-4 0.0 0.0 0.23466388156440393 0.0 46-47 2.7426821127209437E-4 0.0 0.0 0.37895638751465277 0.0 48-49 2.7426821127209437E-4 0.0 0.0 0.753003374047535 0.0 50-51 2.7426821127209437E-4 0.0 0.0 0.9372841852012552 0.0 52-53 2.7426821127209437E-4 0.0 0.0 1.0930411023826776 0.0 54-55 2.7426821127209437E-4 0.0 0.0 1.2959995787240275 0.0 56-57 2.7426821127209437E-4 0.0 0.0 1.4188168837316715 0.0 58-59 2.7426821127209437E-4 0.0 0.0 1.6072391448756003 0.0 60-61 2.7426821127209437E-4 0.0 0.0 1.9544627003460717 0.0 62-63 2.7426821127209437E-4 0.0 0.0 2.1234667721319362 0.0 64-65 2.7426821127209437E-4 0.0 0.0 2.311505057780084 0.0 66-67 2.7426821127209437E-4 0.0 0.0 2.854309274708686 0.0 68-69 2.7426821127209437E-4 0.0 0.0 3.3639544648944915 0.0 70-71 3.0169503239930377E-4 0.0 0.0 3.6634004979613644 0.0 72-73 3.2912185352651323E-4 0.0 0.0 3.967701078367753 0.0 74-75 3.2912185352651323E-4 0.0 0.0 4.180862332168425 0.0 76-77 3.2912185352651323E-4 0.0 0.0 4.295780712691433 0.0 78-79 3.2912185352651323E-4 0.0 0.0 4.484641802973396 0.0 80-81 3.2912185352651323E-4 0.0 0.0 4.8570980338809004 0.0 82-83 3.2912185352651323E-4 0.0 0.0 5.323792822181496 0.0 84-85 3.2912185352651323E-4 0.0 0.0 5.970188142507569 0.0 86-87 3.2912185352651323E-4 0.0 0.0 7.327239825060764 0.0 88-89 3.2912185352651323E-4 0.0 0.0 8.910233660059912 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2565 0.0 57.053677 2 TATAACG 425 0.0 53.66271 2 ACCTGGG 2815 0.0 50.293343 3 GTACCTG 3345 0.0 45.052803 1 TAACGCA 520 0.0 44.75303 4 ATAACGC 585 0.0 38.98145 3 CCTGGGG 1880 0.0 37.38811 4 GTACACG 290 0.0 36.06479 1 TCACGCA 220 0.0 34.540367 4 CTAACGC 85 1.6306934E-5 33.53551 3 TACACGG 285 0.0 33.343056 2 GTATAAC 730 0.0 33.21284 1 ACCGATC 95 3.494812E-5 29.996407 8 TACGCAG 540 0.0 29.903004 4 AGTACGC 530 0.0 29.584099 2 GTACCCG 250 0.0 28.523968 1 GACCGAT 100 4.9587303E-5 28.496586 7 GAGTACG 655 0.0 27.580427 1 GTACGCA 605 0.0 25.128538 3 GTACCGG 160 1.8469982E-6 23.769974 1 >>END_MODULE