##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003856_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1859554 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.989672792508312 31.0 31.0 34.0 28.0 34.0 2 30.919260747469554 31.0 31.0 34.0 27.0 34.0 3 31.01594791009027 31.0 31.0 34.0 27.0 34.0 4 34.488442927712775 37.0 35.0 37.0 32.0 37.0 5 34.47531719971563 37.0 35.0 37.0 32.0 37.0 6 34.583228021342755 37.0 35.0 37.0 32.0 37.0 7 34.60951443195519 37.0 35.0 37.0 32.0 37.0 8 34.59951579787411 37.0 35.0 37.0 32.0 37.0 9 36.0704900207254 39.0 37.0 39.0 32.0 39.0 10-11 35.90416868776062 39.0 35.0 39.0 30.5 39.0 12-13 35.7960295855888 39.0 35.0 39.0 30.0 39.0 14-15 36.78248225112043 39.0 36.0 41.0 31.0 41.0 16-17 36.86644808378783 39.0 36.0 41.0 31.0 41.0 18-19 36.85194810153402 39.5 36.0 41.0 31.0 41.0 20-21 36.75215024677961 39.5 36.0 41.0 30.0 41.0 22-23 36.660249446910385 39.0 36.0 41.0 30.0 41.0 24-25 36.41465937531258 39.0 36.0 41.0 29.5 41.0 26-27 36.270118533798964 39.0 36.0 41.0 29.5 41.0 28-29 36.14415741624067 39.0 36.0 40.5 29.0 41.0 30-31 35.89182217886655 39.0 35.0 40.0 28.0 41.0 32-33 35.62641714088432 38.0 35.0 40.0 27.0 41.0 34-35 35.56934754247524 38.0 35.0 40.0 27.0 41.0 36-37 35.424119708274134 38.0 35.0 40.0 26.5 41.0 38-39 35.413345081670116 38.5 35.0 40.0 26.5 41.0 40-41 35.61186284453154 39.0 35.0 40.5 27.0 41.0 42-43 35.44572784656966 39.0 35.0 41.0 26.5 41.0 44-45 35.19288926269417 39.0 35.0 40.0 25.0 41.0 46-47 34.89274014091551 38.0 34.5 40.0 24.0 41.0 48-49 34.59780705480992 38.0 34.0 40.0 23.0 41.0 50-51 33.77867004668862 37.5 33.5 39.5 22.5 40.5 52-53 33.94393628794862 38.0 33.5 39.5 23.0 40.5 54-55 34.291017899991076 38.0 34.0 40.0 22.5 41.0 56-57 33.93413286196582 38.0 33.5 40.0 20.5 41.0 58-59 33.623442771761404 38.0 33.0 40.0 19.0 41.0 60-61 33.24271653310417 38.0 32.0 40.0 17.5 41.0 62-63 33.08346356169275 37.0 32.0 40.0 16.5 41.0 64-65 32.815091952156266 37.0 32.0 39.5 13.5 41.0 66-67 32.28577416950516 36.0 31.0 39.0 11.0 41.0 68-69 31.860329412321448 36.0 31.0 39.0 8.0 40.5 70-71 31.29992810103928 35.0 30.0 38.5 7.5 40.0 72-73 30.92104074417844 35.0 30.0 37.0 6.5 40.0 74-75 30.587880481018566 35.0 30.0 37.0 2.0 39.0 76-77 30.174301741170193 34.5 29.5 36.5 2.0 39.0 78-79 29.550512649807427 34.0 29.0 36.0 2.0 39.0 80-81 28.96054860466542 34.0 28.0 36.0 2.0 37.5 82-83 28.547957736102312 34.0 26.5 35.0 2.0 37.0 84-85 28.269056988933905 34.0 26.5 35.0 2.0 37.0 86-87 28.220564984937248 34.0 27.0 35.0 2.0 36.0 88-89 27.811918341709895 34.0 26.0 35.0 2.0 36.0 90-91 27.360468424149015 34.0 25.0 35.0 2.0 35.5 92-93 27.064856411806275 33.0 25.0 35.0 2.0 35.0 94-95 26.832826849879055 33.0 25.0 35.0 2.0 35.0 96-97 26.575300851709606 33.0 25.0 35.0 2.0 35.0 98-99 26.329438402971896 33.0 24.5 35.0 2.0 35.0 100-101 26.03459781216356 33.0 23.5 35.0 2.0 35.0 102-103 25.45357220064596 32.0 19.5 35.0 2.0 35.0 104-105 24.98334062898953 32.0 18.5 35.0 2.0 35.0 106-107 24.485182737366056 31.5 16.0 34.0 2.0 35.0 108-109 24.20458319575554 31.0 10.5 34.0 2.0 35.0 110-111 23.856267416810695 31.0 4.5 34.0 2.0 35.0 112-113 23.135386764783384 31.0 2.0 34.0 2.0 35.0 114-115 22.542262553278903 31.0 2.0 34.0 2.0 35.0 116-117 21.79476691722854 30.0 2.0 34.0 2.0 35.0 118-119 21.027085795841366 29.5 2.0 34.0 2.0 35.0 120-121 19.401836139203272 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5262270542774523 1101 2 0.509433267922855 1101 3 0.5133709832230657 1101 4 0.7740672196563594 1101 5 0.8386939322921805 1101 6 0.8125754628102726 1101 7 0.6643408389247298 1101 8 0.6814639190072072 1101 9 0.6854162127655243 1101 10-11 0.8396547404325858 1101 12-13 0.9853626490221572 1101 14-15 1.0776890009788218 1101 16-17 0.9473566087299048 1101 18-19 0.9686404520084793 1101 20-21 0.9249318789803809 1101 22-23 0.9048659656556239 1101 24-25 0.9449325920225888 1101 26-27 0.9109460697391185 1101 28-29 0.9829490234860359 1101 30-31 0.8786927501888968 1101 32-33 1.0137322932906372 1101 34-35 0.9916335194184924 1101 36-37 0.8335651027930808 1101 38-39 0.8569036091660891 1101 40-41 0.9450944611256489 1101 42-43 0.9309577090775889 1101 44-45 1.0381525031453833 1101 46-47 0.9193299652705278 1101 48-49 1.0306355726742709 1101 50-51 0.7732847499134081 1101 52-53 0.8545392225576975 1101 54-55 0.9337805221113982 1101 56-57 0.9797219336044733 1101 58-59 1.0495021141791625 1101 60-61 0.8381326540152614 1101 62-63 1.0152360118810009 1101 64-65 0.9570488300724804 1101 66-67 0.9902961021573304 1101 68-69 0.8992497523911851 1101 70-71 0.530644006391416 1101 72-73 0.5436376181565876 1101 74-75 0.5000720507493455 1101 76-77 0.49277731445284445 1101 78-79 0.6718736759033348 1101 80-81 0.6913676286037784 1101 82-83 0.7399924760577292 1101 84-85 0.8475141232794066 1101 86-87 0.8732869754538441 1101 88-89 0.7306333921761983 1101 90-91 0.656190453872064 1101 92-93 0.6513889680019425 1101 94-95 0.4719741616503583 1101 96-97 0.4226295351473901 1101 98-99 0.4359548711099386 1101 100-101 0.5232687635424682 1101 102-103 0.34642557261541285 1101 104-105 0.46047212130725157 1101 106-107 0.3071959569433247 1101 108-109 0.45779737337833737 1101 110-111 0.40079410645325453 1101 112-113 0.05954919212283727 1101 114-115 0.11249212272095477 1101 116-117 -0.0037765730294871958 1101 118-119 0.13370668268155939 1101 120-121 0.4933770215069302 1102 1 0.5840305282795732 1102 2 0.6601913744791013 1102 3 0.6706306065270198 1102 4 0.7876895486417581 1102 5 0.927127891536621 1102 6 0.8771246968852537 1102 7 0.6959513045674441 1102 8 0.7083569133668917 1102 9 0.7492009752564286 1102 10-11 0.8274491730880911 1102 12-13 0.829458726527811 1102 14-15 0.9182116416000028 1102 16-17 0.9506804563340978 1102 18-19 0.8751069527500661 1102 20-21 0.9349031712013058 1102 22-23 0.770720748798631 1102 24-25 0.9212553567367436 1102 26-27 0.954742389516781 1102 28-29 1.0861101702869718 1102 30-31 1.0982669983220816 1102 32-33 0.7626901661618177 1102 34-35 0.7355809793714414 1102 36-37 1.0094149509512462 1102 38-39 0.8996004163277931 1102 40-41 0.9053966512632243 1102 42-43 1.0555190483997308 1102 44-45 0.954839050082704 1102 46-47 1.0528427723413571 1102 48-49 0.9732322935322131 1102 50-51 0.7799548313089133 1102 52-53 0.9444103248969853 1102 54-55 0.8946648366446794 1102 56-57 0.9938217232077236 1102 58-59 1.009177908730095 1102 60-61 1.158810691528494 1102 62-63 0.9440995587150809 1102 64-65 1.001591161182894 1102 66-67 0.9939125572745269 1102 68-69 0.8156624026584609 1102 70-71 0.7932013208775857 1102 72-73 0.6713955880568534 1102 74-75 0.545046485389534 1102 76-77 0.4841034187183517 1102 78-79 0.7234095186948295 1102 80-81 0.7889629678664427 1102 82-83 0.8799249399382845 1102 84-85 0.7652221652999529 1102 86-87 0.8315556143770095 1102 88-89 0.7045254179156366 1102 90-91 0.7591824513113536 1102 92-93 0.7393610868411891 1102 94-95 0.3845678787497633 1102 96-97 0.4567518479278654 1102 98-99 0.6689303428654227 1102 100-101 0.6671630813601688 1102 102-103 0.6135179276656864 1102 104-105 0.6631969212265325 1102 106-107 0.6125696108067658 1102 108-109 0.6634885101522592 1102 110-111 0.6532059675239061 1102 112-113 0.7599091586705242 1102 114-115 0.7490848681930125 1102 116-117 0.8304166237748376 1102 118-119 0.5971626522256521 1102 120-121 0.8237028374249817 1103 1 0.5860125194089818 1103 2 0.5478741746692535 1103 3 0.6756051531899345 1103 4 0.8252343977447794 1103 5 0.9005930991555431 1103 6 0.8776347789913075 1103 7 0.7801541743562055 1103 8 0.7231649864362026 1103 9 0.8490195942686682 1103 10-11 0.9424418578497793 1103 12-13 0.9078115287025454 1103 14-15 1.0661925896733422 1103 16-17 0.9450182311170678 1103 18-19 0.8789021076162058 1103 20-21 1.0168107638186896 1103 22-23 0.9820995673761033 1103 24-25 1.075054896130574 1103 26-27 1.0971881252174498 1103 28-29 1.096687035672737 1103 30-31 1.0754413317584905 1103 32-33 1.0137710214677895 1103 34-35 0.9932380723834271 1103 36-37 0.8733566875011363 1103 38-39 0.8100243309264314 1103 40-41 1.0194300936713105 1103 42-43 1.1031224655621514 1103 44-45 1.0843186758748047 1103 46-47 1.123712893400473 1103 48-49 1.2632577237928402 1103 50-51 1.0043321591469763 1103 52-53 1.1678184413500432 1103 54-55 0.9955548496136544 1103 56-57 1.184088164032751 1103 58-59 1.2403495006828749 1103 60-61 1.1583944844680119 1103 62-63 1.214730884550093 1103 64-65 1.1731628989789087 1103 66-67 1.2719038897555706 1103 68-69 1.4143794437022592 1103 70-71 1.2773203769579986 1103 72-73 0.9475980332387195 1103 74-75 0.8900814923817002 1103 76-77 0.9493551598373706 1103 78-79 0.9805556065423033 1103 80-81 0.9855558667776982 1103 82-83 0.921606383232497 1103 84-85 0.9222526862279921 1103 86-87 1.1070815500121434 1103 88-89 1.1632766121225195 1103 90-91 1.0530607275919834 1103 92-93 0.9171605154680016 1103 94-95 0.9430711394537781 1103 96-97 0.8745509073312938 1103 98-99 0.9796739126094103 1103 100-101 1.0665071560171384 1103 102-103 0.9523887502492343 1103 104-105 1.1273889912312818 1103 106-107 0.8831180459919068 1103 108-109 0.9711011578993016 1103 110-111 1.1730877237349375 1103 112-113 1.081618570636131 1103 114-115 1.055190069165917 1103 116-117 0.979935387245721 1103 118-119 0.7856600515084367 1103 120-121 0.9209912608872841 1104 1 0.5278174401519529 1104 2 0.6434727354005574 1104 3 0.7141911689440903 1104 4 0.9557274445210098 1104 5 1.0738411785098165 1104 6 0.8220128787676373 1104 7 0.6486351623947115 1104 8 0.7944826952760309 1104 9 0.9440939889005833 1104 10-11 0.9073168943177379 1104 12-13 0.9822265826751178 1104 14-15 1.038344199125838 1104 16-17 1.0154422318950438 1104 18-19 1.0323252331632204 1104 20-21 0.9289886302000951 1104 22-23 0.8973353934968813 1104 24-25 1.1422215790052164 1104 26-27 1.1195789644667045 1104 28-29 1.1535381491610153 1104 30-31 1.130535175967374 1104 32-33 1.0903566482304115 1104 34-35 1.1022033548885375 1104 36-37 1.157438667468071 1104 38-39 1.018678906439412 1104 40-41 1.2986219637481327 1104 42-43 1.2548763311655762 1104 44-45 1.160518228534535 1104 46-47 1.388421635985317 1104 48-49 1.4417756660276027 1104 50-51 1.2136164147144157 1104 52-53 1.0878072578432878 1104 54-55 1.301961540268124 1104 56-57 1.3740901089904511 1104 58-59 1.3483758548596683 1104 60-61 1.0596285601268747 1104 62-63 1.164825214998416 1104 64-65 1.2729382563648883 1104 66-67 1.3783363510995343 1104 68-69 1.212178237828489 1104 70-71 1.1768458072798929 1104 72-73 1.0844909633174922 1104 74-75 1.2597046568278714 1104 76-77 1.3506845384233905 1104 78-79 1.3759825247568358 1104 80-81 1.2435238722235802 1104 82-83 1.170669886101308 1104 84-85 1.230540150975635 1104 86-87 1.142456440608271 1104 88-89 1.1938547507980068 1104 90-91 1.3184696549478119 1104 92-93 1.3068775241230632 1104 94-95 1.323441167655666 1104 96-97 1.265055137614283 1104 98-99 1.282267027459163 1104 100-101 1.2348515107774176 1104 102-103 1.2080398248383624 1104 104-105 1.266584264984079 1104 106-107 1.18585071155643 1104 108-109 1.3712718279372318 1104 110-111 1.546998547424522 1104 112-113 1.4572336635078607 1104 114-115 1.453338955677637 1104 116-117 1.0582646685838526 1104 118-119 0.9252584177439722 1104 120-121 0.8942053034818542 1105 1 0.7059998028678791 1105 2 0.8825672981396693 1105 3 0.8214765961574457 1105 4 1.0056211725420354 1105 5 1.0976277457316783 1105 6 0.9828555189500392 1105 7 0.8997973914011652 1105 8 0.9683929244370617 1105 9 1.1191645148502687 1105 10-11 1.0107857083224516 1105 12-13 1.2078429601531795 1105 14-15 1.3511543200826708 1105 16-17 1.0966958548034214 1105 18-19 0.9536351802204521 1105 20-21 1.13230334983529 1105 22-23 1.1715939049162856 1105 24-25 1.274324894690963 1105 26-27 1.355658301329548 1105 28-29 1.5027531856799357 1105 30-31 1.2740714243732967 1105 32-33 1.3182651190732457 1105 34-35 1.138866221526861 1105 36-37 1.1357364801975223 1105 38-39 0.9670399026909067 1105 40-41 0.9557407371762707 1105 42-43 1.1256273842242734 1105 44-45 0.9154492483590886 1105 46-47 1.0865440033385667 1105 48-49 1.1621330297096364 1105 50-51 0.9139468239581419 1105 52-53 0.9515303517104243 1105 54-55 1.0320268492297515 1105 56-57 0.9358766375679508 1105 58-59 0.925987247215815 1105 60-61 0.9440215539051309 1105 62-63 1.1635460868150815 1105 64-65 0.9926531576285527 1105 66-67 1.0323679235383807 1105 68-69 0.967971026791659 1105 70-71 0.5193826160289738 1105 72-73 0.6372255345583611 1105 74-75 0.6315232602208489 1105 76-77 0.9698226726738817 1105 78-79 1.0898176872563496 1105 80-81 1.0049302369490114 1105 82-83 1.2125961140257218 1105 84-85 1.081049773420279 1105 86-87 1.0550303479391054 1105 88-89 1.2445619425126573 1105 90-91 1.3071768080181059 1105 92-93 1.2810751706817847 1105 94-95 1.1276732076912168 1105 96-97 1.1475685665279904 1105 98-99 1.470291624591752 1105 100-101 1.3587197142013103 1105 102-103 1.1999230609167313 1105 104-105 1.1400718215014507 1105 106-107 1.1763272930766995 1105 108-109 1.2920571778618744 1105 110-111 1.4529290069059115 1105 112-113 1.5638172126055174 1105 114-115 1.5025955348792444 1105 116-117 1.6537239567548134 1105 118-119 1.7571153756602662 1105 120-121 1.762764738501371 1106 1 0.4262833034772342 1106 2 0.7403793909719383 1106 3 0.6037509314622937 1106 4 0.7622325894265103 1106 5 0.6222344957336503 1106 6 0.551069192678888 1106 7 0.6539131579886046 1106 8 0.60942825902859 1106 9 0.7852369633974305 1106 10-11 0.8446693350139682 1106 12-13 0.9136046385462819 1106 14-15 0.9968570307175284 1106 16-17 0.8668979547689375 1106 18-19 0.736086695180532 1106 20-21 0.9274612947223844 1106 22-23 0.7364780159317661 1106 24-25 1.0384573164950197 1106 26-27 1.0499761810434833 1106 28-29 0.9138196920458981 1106 30-31 0.8292466825258558 1106 32-33 0.9306866796492699 1106 34-35 0.8246870453513893 1106 36-37 0.9769761060572577 1106 38-39 0.8195101193995598 1106 40-41 0.9103987031209257 1106 42-43 0.7339909220002312 1106 44-45 0.9100960208926523 1106 46-47 0.9998199087479875 1106 48-49 0.9310511118394018 1106 50-51 1.014365079812805 1106 52-53 0.7898048094123666 1106 54-55 1.0431794612888936 1106 56-57 1.07937077172042 1106 58-59 1.2847525238200745 1106 60-61 1.0473850719396225 1106 62-63 1.117456344743914 1106 64-65 0.9020663162367555 1106 66-67 0.9688102745100551 1106 68-69 0.8136588984247481 1106 70-71 0.7238071391409697 1106 72-73 0.9813477434441253 1106 74-75 0.9433499117260702 1106 76-77 1.0186220954334217 1106 78-79 1.0546178312374437 1106 80-81 0.8245246616958575 1106 82-83 1.065538316278353 1106 84-85 1.063421138029355 1106 86-87 1.0351911619192222 1106 88-89 0.9965561595792849 1106 90-91 0.8714731168904493 1106 92-93 0.7940112596605182 1106 94-95 0.7383871314786319 1106 96-97 1.0120582101550504 1106 98-99 0.9110394247344189 1106 100-101 0.7258914004646719 1106 102-103 0.7080126200503187 1106 104-105 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-1.1565155018235274 2316 76-77 -1.6110987510494716 2316 78-79 -1.3543510765545825 2316 80-81 -1.5653236440592302 2316 82-83 -1.4218937220561116 2316 84-85 -1.2376529439828161 2316 86-87 -1.2858533504316298 2316 88-89 -1.2259919050239212 2316 90-91 -1.408271836224614 2316 92-93 -1.117945202093356 2316 94-95 -1.0047403393442167 2316 96-97 -1.2529977399050765 2316 98-99 -1.041634785789423 2316 100-101 -0.76950716323352 2316 102-103 -0.7424748815058102 2316 104-105 -0.8800431366124819 2316 106-107 -0.980318730148916 2316 108-109 -0.8610716090073147 2316 110-111 -1.0792516331466508 2316 112-113 -1.0510364960856506 2316 114-115 -0.878187043430497 2316 116-117 -1.0668620452720816 2316 118-119 -0.756150288612762 2316 120-121 -1.2271234491010965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 24562.0 3 3565.0 4 2988.0 5 3476.0 6 4062.0 7 4405.0 8 5348.0 9 6625.0 10 8668.0 11 10944.0 12 12273.0 13 14437.0 14 18253.0 15 18873.0 16 14894.0 17 14947.0 18 15762.0 19 17040.0 20 18543.0 21 20465.0 22 22402.0 23 25051.0 24 27641.0 25 31636.0 26 36198.0 27 41613.0 28 48993.0 29 57335.0 30 68314.0 31 80984.0 32 98121.0 33 120100.0 34 150914.0 35 190676.0 36 246230.0 37 269138.0 38 103484.0 39 594.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.72059292403633 18.372971483228767 14.737015601273177 21.169419991461723 2 13.510479204379214 21.475206306623082 37.60695169755297 27.407362791444733 3 23.74145671283653 23.47861554407742 31.57500221023063 21.204925532855416 4 10.576139341999728 15.129982358820401 44.212169214324874 30.081709084854996 5 10.291540683090858 38.604422870934066 37.41217306414654 13.691863381828536 6 26.34073027887971 36.62697547477023 18.25409739148923 18.778196854860845 7 22.899860899085407 33.090507786126246 24.79076565156441 19.21886566322394 8 21.533479242630964 28.57866412735322 22.15189461559986 27.735962014415954 9 19.765225346681987 20.36501460698348 28.345357137627776 31.52440290870676 10-11 24.19295581297675 22.990011981717508 28.682941087313075 24.134091117992668 12-13 27.98023938554348 22.36230938555975 27.939457833122106 21.717993395774663 14-15 22.70061918791072 24.19263563631828 31.989386899572793 21.11735827619821 16-17 18.933772179856923 28.67110431048464 30.95609130076084 21.439032208897597 18-19 17.40821367100557 31.80100449895088 30.225110303641884 20.565671526401665 20-21 20.06134738008223 26.733200471877883 27.471832042287808 25.733620105752074 22-23 18.176386738857108 26.44794922224597 30.499332299871668 24.876331739025257 24-25 23.155654344451424 25.593601213237566 28.569119502381675 22.68162493992934 26-27 20.92200937771798 23.345061130907553 34.73069774745468 21.002231743919793 28-29 18.11272656945707 30.108420247740987 30.85961244732039 20.919240735481555 30-31 18.86897584437533 26.273599173155777 29.893294233656736 24.964130748812156 32-33 20.382796094455514 25.8038237833694 31.67063129399848 22.142748828176607 34-35 23.652455399714434 24.689509196778424 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21.842851671048503 60-61 21.752555556942372 25.255664227738016 33.568353584780134 19.42342663053948 62-63 19.130763445189817 27.397684365669654 31.88780742986947 21.583744759271056 64-65 17.899112014259863 30.92808482122267 29.716023625358464 21.456779539159005 66-67 19.390319870983706 26.722098381435895 27.08883653088556 26.798745216694847 68-69 18.303141972888152 26.79880220509321 29.924656149553403 24.973399672465234 70-71 22.659658588266527 25.658459599901086 27.959122015863997 23.722759795968393 72-73 20.34537715870904 23.578747800130355 33.86975360102678 22.20612144013382 74-75 18.259689011256906 29.597519387252603 30.330415359297387 21.812376242193114 76-77 18.740518199084967 26.38633838775991 29.40522977315351 25.467913640001605 78-79 20.098066489905246 25.700059086335802 30.709586995469323 23.492287428289625 80-81 22.973682060308313 25.09342840087659 28.57371268055563 23.359176858259474 82-83 20.262592760796384 24.301815270794883 33.59767066805689 21.837921300351837 84-85 20.553976447805407 26.611798024490067 30.405528258128665 22.428697269575856 86-87 19.593558323826972 28.510587607034743 29.99463808716473 21.901215981973554 88-89 18.79318452741952 26.61709544901597 27.30690819474531 27.2828118288192 90-91 19.252669010172347 24.04930209105369 29.32457806992143 27.37345082885253 92-93 21.818494130187137 25.21818792627443 30.49909283970209 22.46422510383634 94-95 20.20878438936183 24.405854233525165 30.94775967327783 24.437601703835178 96-97 15.409150979492928 26.54736545031709 35.17300892520359 22.87047464498639 98-99 18.503285478183713 28.75030230090686 28.88156400107159 23.864848219837835 100-101 16.324736010317764 26.025920886824032 30.846224432551438 26.803118670306763 102-103 20.198961585614374 23.93915827326026 29.523199883643752 26.338680257481617 104-105 22.81484142544801 24.309148066406873 29.0081025157359 23.867907992409215 106-107 20.76398600544739 24.656010662516273 33.204501830720616 21.375501501315718 108-109 19.64167682374227 26.031137632263572 30.741017907204665 23.586167636789487 110-111 18.665160212683517 28.020613890851546 29.066584614850292 24.24764128161464 112-113 20.59053465390597 24.880975607902922 26.763413021221005 27.765076716970107 114-115 19.042372736476796 24.700302214657885 28.982676047432804 27.274649001432515 116-117 23.080896097047415 24.509325229402247 26.788732264395364 25.621046409154975 118-119 21.20029213855792 23.3246746620282 31.352378429622263 24.122654769791612 120-121 18.714935004847874 28.828334266364656 29.005967510971825 23.45076321781564 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4555.0 1 4731.0 2 3031.0 3 1159.0 4 1097.0 5 1024.0 6 1075.5 7 1219.5 8 1439.5 9 1543.0 10 1408.5 11 1306.5 12 1369.0 13 1501.0 14 1644.5 15 1815.0 16 2008.0 17 2209.5 18 2407.0 19 2675.0 20 2983.0 21 3370.5 22 4037.0 23 4851.5 24 5932.5 25 7452.0 26 9424.0 27 12043.5 28 15442.5 29 19578.5 30 24531.0 31 29256.0 32 33043.5 33 36240.5 34 39102.0 35 41925.0 36 44567.0 37 47628.0 38 50120.5 39 50736.0 40 51578.0 41 52295.0 42 52908.5 43 54922.5 44 56305.0 45 58914.5 46 70823.0 47 120915.5 48 220107.5 49 212876.0 50 109497.0 51 59901.5 52 42527.5 53 34780.0 54 29939.5 55 25403.5 56 21004.0 57 16535.0 58 12758.0 59 9990.5 60 7802.5 61 5868.0 62 4329.0 63 3016.5 64 1937.0 65 1267.0 66 813.0 67 495.5 68 282.0 69 176.0 70 108.5 71 61.5 72 43.5 73 36.0 74 25.5 75 17.5 76 14.5 77 12.5 78 9.5 79 6.5 80 4.5 81 5.5 82 8.5 83 6.5 84 4.0 85 3.5 86 2.0 87 1.5 88 1.0 89 0.5 90 1.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4874287060230571 2 0.8962364093755815 3 0.8526238011910383 4 0.8374588745473376 5 0.8458479829034273 6 0.8507416294444797 7 0.837243769204874 8 0.8327803333487492 9 0.8374588745473376 10-11 0.8332374322014848 12-13 0.8379966379034973 14-15 0.8392603817904724 16-17 0.8568183553690831 18-19 0.8790548701462824 20-21 0.8755056319956291 22-23 0.855689052321148 24-25 0.8546135256088288 26-27 0.8565763618588114 28-29 0.8721177228518235 30-31 0.8820125686051601 32-33 0.8808294892216091 34-35 0.8897025845982424 36-37 0.8963977383824293 38-39 0.8876590838448359 40-41 0.8926602830571201 42-43 0.8883043998722273 44-45 0.8803723903688734 46-47 0.8790548701462824 48-49 0.8831687598209033 50-51 0.9248454199232718 52-53 0.8903747887934419 54-55 0.8957793105228459 56-57 0.9183922596493567 58-59 0.8787053239647787 60-61 0.9037113200261999 62-63 0.9021518062933369 64-65 0.8937089216016313 66-67 0.8973119360879007 68-69 0.9142783699747359 70-71 0.9456299736388403 72-73 0.9509538308648202 74-75 0.9545837335188976 76-77 0.9608755647859648 78-79 0.9321858897348503 80-81 0.9483725667552543 82-83 0.9492867644607255 84-85 0.9191989046835961 86-87 0.9103258093069628 88-89 0.9116433295295538 90-91 0.9226674783308255 92-93 0.9137137184507683 94-95 0.9081478677145165 96-97 0.9152194558480151 98-99 0.9374559706252145 100-101 0.930034836310212 102-103 0.9108097963275065 104-105 0.9030660039988083 106-107 0.9054321627659105 108-109 0.9051632810878307 110-111 0.9043028597179753 112-113 0.9125575272350253 114-115 0.9136330539473443 116-117 0.931433021036227 118-119 0.9291206386047407 120-121 0.9138481592898082 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1859554.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.58277298796789 #Duplication Level Percentage of deduplicated Percentage of total 1 84.81764587171833 43.75129372374699 2 9.612752692745138 9.917048798786992 3 2.3368730542007157 3.6162717676960408 4 0.9544431447267253 1.9693129625744346 5 0.5107918533760202 1.3174030108399313 6 0.3259998609139103 1.0089586091778808 7 0.22945433905913865 0.8285123757954235 8 0.1769283346780329 0.7301163298268942 9 0.1318784210052639 0.6122389187453565 >10 0.8092598089907618 7.699895367638629 >50 0.05718900639274195 2.021251228409714 >100 0.028787318796354003 2.811000125239986 >500 0.0030919001134403585 1.107457102119929 >1k 0.0028786656228582653 3.3578438112446936 >5k 0.0010661724529104687 4.516282327361999 >10k+ 9.595552076194217E-4 14.735113540795183 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 65179 3.5050877791126256 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 49567 2.6655316274762657 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 38824 2.0878124539540126 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 35771 1.923633301318488 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 24399 1.3120888126937964 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 17502 0.9411934259505237 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 14551 0.7824994595478271 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11888 0.6392930778025269 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 10249 0.5511536637279691 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 9848 0.5295893531459694 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 9810 0.527545852392563 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 9781 0.5259863386597001 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 9433 0.5072721738653462 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 7914 0.4255859200647037 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7891 0.4243490643455366 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 7629 0.4102596644141553 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7493 0.40294608277038474 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 7317 0.3934814477019759 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5004 0.2690967834222615 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4992 0.26845146739486997 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4530 0.24360680034029666 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4195 0.2255917279089502 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3490 0.18767941129969873 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3482 0.18724920061477107 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3362 0.18079604034085595 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3253 0.17493441975871632 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3187 0.17138518160806301 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2962 0.15928550609447212 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2779 0.14944443667675153 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2101 0.11298408112913096 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 5.3776335615959525E-5 0.0 2 0.0 0.0 0.0 5.3776335615959525E-5 0.0 3 0.0 0.0 0.0 5.3776335615959525E-5 0.0 4 0.0 0.0 0.0 5.3776335615959525E-5 0.0 5 0.0 5.3776335615959525E-5 0.0 5.3776335615959525E-5 0.0 6 0.0 5.3776335615959525E-5 0.0 5.3776335615959525E-5 0.0 7 0.0 5.3776335615959525E-5 0.0 5.3776335615959525E-5 0.0 8 0.0 5.3776335615959525E-5 0.0 5.3776335615959525E-5 0.0 9 0.0 5.3776335615959525E-5 0.0 5.3776335615959525E-5 0.0 10-11 0.0 5.3776335615959525E-5 0.0 1.613290068478786E-4 0.0 12-13 0.0 5.3776335615959525E-5 0.0 1.8821717465585834E-4 0.0 14-15 0.0 8.06645034239393E-5 0.0 2.151053424638381E-4 0.0 16-17 0.0 1.0755267123191905E-4 0.0 4.8398702054363576E-4 0.0 18-19 0.0 1.0755267123191905E-4 0.0 7.528686986234334E-4 0.0 20-21 0.0 1.0755267123191905E-4 0.0 0.0013981847260149476 0.0 22-23 0.0 1.613290068478786E-4 0.0 0.002850145787645855 0.0 24-25 0.0 1.613290068478786E-4 0.0 0.004759205702012418 0.0 26-27 0.0 1.613290068478786E-4 0.0 0.007179140804730597 0.0 28-29 0.0 1.613290068478786E-4 0.0 0.014949821301236748 0.0 30-31 8.06645034239393E-5 1.613290068478786E-4 0.0 0.029442543749737843 0.0 32-33 2.151053424638381E-4 1.613290068478786E-4 0.0 0.05587361270498195 0.0 34-35 2.688816780797976E-4 1.613290068478786E-4 0.0 0.07778746946848544 0.0 36-37 2.688816780797976E-4 1.613290068478786E-4 0.0 0.09999709607787674 0.0 38-39 2.688816780797976E-4 1.613290068478786E-4 0.0 0.12720792189955227 0.0 40-41 2.688816780797976E-4 1.613290068478786E-4 0.0 0.15199881261850962 0.0 42-43 2.688816780797976E-4 1.613290068478786E-4 0.0 0.23252887520340898 0.0 44-45 2.688816780797976E-4 1.613290068478786E-4 0.0 0.3134622603054281 0.0 46-47 2.688816780797976E-4 1.8821717465585834E-4 0.0 0.37549326343843736 0.0 48-49 2.688816780797976E-4 2.151053424638381E-4 0.0 0.4458595985919204 0.0 50-51 2.688816780797976E-4 3.226580136957572E-4 0.0 0.5231630810398622 0.0 52-53 2.688816780797976E-4 3.226580136957572E-4 0.0 0.7059488457985086 0.0 54-55 2.688816780797976E-4 3.226580136957572E-4 0.0 0.819766460129687 0.0 56-57 2.688816780797976E-4 3.226580136957572E-4 0.0 1.1396281043734142 0.0 58-59 2.688816780797976E-4 3.226580136957572E-4 0.0 1.4721809638225078 0.0 60-61 2.688816780797976E-4 3.226580136957572E-4 0.0 1.6986600012691215 0.0 62-63 2.688816780797976E-4 3.226580136957572E-4 0.0 2.1592812039876232 0.0 64-65 2.688816780797976E-4 3.226580136957572E-4 0.0 2.3351029332840025 0.0 66-67 2.688816780797976E-4 3.226580136957572E-4 0.0 3.049064453089289 0.0 68-69 2.688816780797976E-4 3.226580136957572E-4 0.0 3.7161867845730754 0.0 70-71 2.688816780797976E-4 3.226580136957572E-4 0.0 3.952345562430561 0.0 72-73 2.688816780797976E-4 3.226580136957572E-4 0.0 4.48274156061077 0.0 74-75 2.688816780797976E-4 3.226580136957572E-4 0.0 4.941426815247096 0.0 76-77 2.688816780797976E-4 3.226580136957572E-4 0.0 5.101330749201152 0.0 78-79 2.688816780797976E-4 3.226580136957572E-4 0.0 5.334693157606609 0.0 80-81 2.688816780797976E-4 3.226580136957572E-4 0.0 5.791926451181304 0.0 82-83 2.688816780797976E-4 3.226580136957572E-4 0.0 6.10009174242856 0.0 84-85 2.688816780797976E-4 3.226580136957572E-4 0.0 6.790794997079946 0.0 86-87 2.688816780797976E-4 3.226580136957572E-4 0.0 8.305566818710293 0.0 88-89 2.688816780797976E-4 3.226580136957572E-4 0.0 10.421638737030491 0.0 90-91 2.688816780797976E-4 3.226580136957572E-4 0.0 11.888952942479756 0.0 92-93 2.688816780797976E-4 3.226580136957572E-4 0.0 12.962597483052388 0.0 94-95 2.688816780797976E-4 3.495461815037369E-4 0.0 13.771581787891074 0.0 96-97 2.688816780797976E-4 4.0332251711969647E-4 0.0 14.655906738927722 0.0 98-99 2.688816780797976E-4 4.302106849276762E-4 0.0 15.39379872808211 0.0 100-101 2.688816780797976E-4 4.302106849276762E-4 0.0 15.767436707941798 0.0 102-103 2.688816780797976E-4 4.570988527356559E-4 0.0 16.201115966516703 0.0 104-105 2.688816780797976E-4 4.839870205436357E-4 0.0 16.71247514188886 0.0 106-107 2.688816780797976E-4 4.839870205436357E-4 0.0 17.375940682550763 0.0 108-109 2.688816780797976E-4 4.839870205436357E-4 0.0 18.14166192538641 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1425 0.0 41.173065 2 ACCTGGG 1550 0.0 36.321823 3 TATAACG 205 3.6379788E-12 33.670944 2 GTACCGG 140 1.0654185E-7 32.941803 1 TACCGCG 165 1.3315002E-8 31.324945 8 TATAGCG 75 0.004642767 30.64048 5 GTACACG 230 1.0913936E-11 30.0773 1 GAGTACG 720 0.0 28.824078 1 AGTACGC 700 0.0 27.938868 2 GTACGCA 700 0.0 26.261843 3 ACCGGGA 110 9.5166336E-4 26.112629 3 GTACCTG 2370 0.0 25.783562 1 TACGCAG 760 0.0 24.944431 4 TACACGG 235 1.4922989E-8 24.477102 2 GTACATG 4820 0.0 24.039999 1 GAGTACT 4925 0.0 23.453972 12-13 GTACCCG 200 2.404795E-6 23.059263 1 TCTCGGG 840 0.0 22.928156 114-115 CCTGGGG 1360 0.0 22.810146 4 TACCGGG 180 2.8285378E-5 22.369352 2 >>END_MODULE