##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003856_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1859554 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62192224587186 31.0 31.0 34.0 30.0 34.0 2 31.82543986353717 31.0 31.0 34.0 30.0 34.0 3 31.906819054461447 31.0 31.0 34.0 30.0 34.0 4 35.53341231284491 37.0 35.0 37.0 33.0 37.0 5 35.458504566148655 37.0 35.0 37.0 33.0 37.0 6 35.5391776737863 37.0 35.0 37.0 33.0 37.0 7 35.47954025535155 37.0 35.0 37.0 33.0 37.0 8 35.50153316332841 37.0 35.0 37.0 33.0 37.0 9 37.033880166964764 39.0 37.0 39.0 33.0 39.0 10-11 36.92931665334806 39.0 37.0 39.0 33.0 39.0 12-13 36.92841563084481 39.0 37.0 39.0 33.0 39.0 14-15 37.849675513590896 39.0 37.0 41.0 32.5 41.0 16-17 38.01349113819765 40.0 37.0 41.0 33.0 41.0 18-19 38.077673194755306 40.0 37.0 41.0 33.0 41.0 20-21 37.968031312884705 40.0 37.0 41.0 32.5 41.0 22-23 37.907120470822576 40.0 37.0 41.0 32.5 41.0 24-25 37.72355494919749 39.5 37.0 41.0 32.0 41.0 26-27 37.37289882412664 39.0 36.5 41.0 31.5 41.0 28-29 37.291747375983704 39.0 36.0 40.0 31.5 41.0 30-31 37.151530958498654 39.0 36.0 40.0 31.0 41.0 32-33 36.91776952968293 39.0 35.5 40.0 31.0 41.0 34-35 36.79402668596879 39.0 35.0 40.0 30.0 41.0 36-37 36.56369618736535 38.5 35.0 40.0 30.0 41.0 38-39 36.40040810861099 38.0 35.0 40.0 30.0 41.0 40-41 36.74185234739083 39.0 35.0 40.5 30.0 41.0 42-43 36.58692406888964 39.0 35.0 41.0 30.0 41.0 44-45 36.456398953727614 39.0 35.0 41.0 30.0 41.0 46-47 36.190470940881525 39.0 35.0 40.0 29.5 41.0 48-49 35.8866991762541 39.0 35.0 40.0 28.0 41.0 50-51 35.467286779518105 38.0 34.0 40.0 27.5 41.0 52-53 35.48049505419041 38.0 34.5 40.0 27.5 41.0 54-55 35.31749548547663 38.0 34.0 40.0 27.0 41.0 56-57 34.906634063866925 38.0 34.0 40.0 26.5 41.0 58-59 34.66131126065713 38.0 34.0 40.0 26.0 41.0 60-61 33.97361706086513 37.0 32.5 40.0 23.5 41.0 62-63 33.996972661186504 37.0 33.0 40.0 25.0 41.0 64-65 33.577798762498965 36.5 32.5 39.5 23.0 41.0 66-67 33.09243721881698 36.0 32.0 39.0 22.0 40.0 68-69 32.739282376311735 36.0 32.0 39.0 22.0 40.0 70-71 32.132757908616796 35.0 31.0 37.5 21.5 40.0 72-73 31.644186455461902 35.0 30.5 37.0 20.0 39.5 74-75 31.32613304050326 34.0 31.0 37.0 19.5 39.0 76-77 30.435582671974032 34.0 29.5 35.5 19.0 38.5 78-79 30.831997887665537 34.0 30.0 36.0 18.5 38.5 80-81 30.481666571661805 34.0 30.0 35.5 17.5 37.5 82-83 30.113390630226387 34.0 29.5 35.0 14.0 37.0 84-85 29.857558586628837 34.0 29.5 35.0 10.5 36.5 86-87 29.725016858881215 34.0 30.0 35.0 7.0 36.0 88-89 29.350102228814006 34.0 29.0 35.0 4.5 36.0 90-91 29.003046967176 34.0 29.0 35.0 2.0 35.5 92-93 28.730495054190413 34.0 29.0 35.0 2.0 35.0 94-95 28.371533442965358 34.0 29.0 35.0 2.0 35.0 96-97 27.78482205948308 34.0 28.0 35.0 2.0 35.0 98-99 27.57740834630239 34.0 28.5 35.0 2.0 35.0 100-101 26.522412363394665 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07247913871360723 1101 2 0.14108900593741325 1101 3 0.13694291011354665 1101 4 0.21924721526562507 1101 5 0.20229400115548657 1101 6 0.16987501821953543 1101 7 0.1121635657481832 1101 8 0.1899421959785883 1101 9 0.23717336584820714 1101 10-11 0.19097672626917017 1101 12-13 0.2957694186363611 1101 14-15 0.2051886912854357 1101 16-17 0.14574194805300777 1101 18-19 0.16910819690327372 1101 20-21 0.15339242141627807 1101 22-23 0.12193977637141984 1101 24-25 0.15996636669782305 1101 26-27 0.18183908119267045 1101 28-29 0.22222429431016621 1101 30-31 0.24118045792933884 1101 32-33 0.33678665120410045 1101 34-35 0.28632005472780975 1101 36-37 0.3112182714344556 1101 38-39 0.1476836334662437 1101 40-41 0.30135633295021336 1101 42-43 0.26004818647148653 1101 44-45 0.20740497199051333 1101 46-47 0.28909613309732407 1101 48-49 0.35711297209483206 1101 50-51 0.47694803423562604 1101 52-53 0.579358984542516 1101 54-55 0.5120527984847243 1101 56-57 0.47521271069528837 1101 58-59 0.2486825135077524 1101 60-61 0.3783664151773678 1101 62-63 0.03717879702945481 1101 64-65 -0.12655701410128728 1101 66-67 -0.1450359803217509 1101 68-69 -0.05738073177067804 1101 70-71 0.25925553025875203 1101 72-73 0.19165937682318912 1101 74-75 -0.07552466129891755 1101 76-77 -0.04667498146210036 1101 78-79 0.06877294294403313 1101 80-81 0.21450440415189576 1101 82-83 0.13894835453062981 1101 84-85 -0.020855665721963845 1101 86-87 -0.2253754479871688 1101 88-89 0.062319220380860685 1101 90-91 0.05659058909357739 1101 92-93 0.1742652177475037 1101 94-95 0.12482566187879485 1101 96-97 0.3728273691547095 1101 98-99 0.14567555737275129 1101 100-101 0.14491614222668403 1102 1 0.03604912745703359 1102 2 0.10944379158632955 1102 3 0.036751859041967094 1102 4 0.07821440346362607 1102 5 -0.028200863887548167 1102 6 0.051397626430855325 1102 7 0.012128883864257034 1102 8 0.06144514778112864 1102 9 0.17623131499948386 1102 10-11 0.11785049189647623 1102 12-13 0.12993755627599768 1102 14-15 0.1871707342221427 1102 16-17 0.14941837810435743 1102 18-19 0.02122136943695807 1102 20-21 0.08359604884730487 1102 22-23 -0.0694247018282752 1102 24-25 0.016491956949742814 1102 26-27 0.024345990004377427 1102 28-29 0.14151782968050242 1102 30-31 0.1117413522007169 1102 32-33 0.03660191504202004 1102 34-35 0.04459751447694771 1102 36-37 0.0033339398609371074 1102 38-39 -0.014389270660402076 1102 40-41 -0.08204187020744769 1102 42-43 -0.07728951784842053 1102 44-45 0.02804199600421242 1102 46-47 0.07639603149076635 1102 48-49 -0.05762923425435673 1102 50-51 0.18421998664860695 1102 52-53 0.06637574778720534 1102 54-55 -0.043585981341109914 1102 56-57 0.2989339331682146 1102 58-59 0.10150511099374881 1102 60-61 0.08737221119509542 1102 62-63 0.008182503446974465 1102 64-65 -0.09564519797006454 1102 66-67 -0.022882492603919502 1102 68-69 0.1258410214553507 1102 70-71 -0.06553931035994864 1102 72-73 -0.03641468938045023 1102 74-75 0.021156352574838166 1102 76-77 -0.08343660899804561 1102 78-79 -0.04692730868339012 1102 80-81 0.10256553314411221 1102 82-83 -0.07076803586710056 1102 84-85 -0.1034297063652545 1102 86-87 -0.14411150047375543 1102 88-89 -0.11028880717218925 1102 90-91 -0.0029117292132774253 1102 92-93 0.12043270251236748 1102 94-95 0.02941999242655058 1102 96-97 0.1849994908674084 1102 98-99 -1.2495453304950388E-5 1102 100-101 0.053584174116643624 1103 1 0.03117094809754306 1103 2 -0.005449849002395979 1103 3 -0.05953273834300887 1103 4 0.04478141058502416 1103 5 0.04647419645740314 1103 6 0.01329358851161544 1103 7 0.0946120957384835 1103 8 0.0803349853689923 1103 9 0.08225104075881973 1103 10-11 0.03080109442289114 1103 12-13 0.06294908066089278 1103 14-15 0.1522273407823178 1103 16-17 0.07600421561838289 1103 18-19 0.03466110064233163 1103 20-21 0.18797143826260054 1103 22-23 0.10417499540854891 1103 24-25 0.07174392883338498 1103 26-27 0.13820903582038824 1103 28-29 0.07517235658996668 1103 30-31 0.1782126381596143 1103 32-33 0.18163230674188213 1103 34-35 0.1346935587099125 1103 36-37 0.19138236350785576 1103 38-39 0.19199977078953623 1103 40-41 0.32662214112770727 1103 42-43 0.3961233523557155 1103 44-45 0.28577524133930154 1103 46-47 0.42842883698850187 1103 48-49 0.5368790294265224 1103 50-51 0.6537503806561134 1103 52-53 0.4743098967965338 1103 54-55 0.4276497631747702 1103 56-57 0.5397106868639128 1103 58-59 0.43977686863552634 1103 60-61 0.5558121039357076 1103 62-63 0.46666536455898466 1103 64-65 0.4668765054420234 1103 66-67 0.19044296396387494 1103 68-69 0.1298471874858791 1103 70-71 0.27767930219087944 1103 72-73 0.2136238483103341 1103 74-75 0.3956104327240091 1103 76-77 0.4842577294228789 1103 78-79 0.5215837518646289 1103 80-81 0.4661318618175372 1103 82-83 0.43233842508060505 1103 84-85 0.29331106651159544 1103 86-87 0.4014591573438544 1103 88-89 0.5032193226652133 1103 90-91 0.3578833933458121 1103 92-93 0.2149695810055654 1103 94-95 0.018447227266470634 1103 96-97 0.38862627987008835 1103 98-99 0.5073541572136442 1103 100-101 0.44324951927128 1104 1 0.03937672462190278 1104 2 -0.03909664468459084 1104 3 0.03269132078370518 1104 4 -0.08727343722203784 1104 5 -0.029861503241129128 1104 6 -0.07780628506613141 1104 7 -0.011904485025887368 1104 8 -0.01767532290680407 1104 9 0.02120368103889092 1104 10-11 0.012622061066863921 1104 12-13 -0.07702417617077373 1104 14-15 -0.05687895115582364 1104 16-17 -0.03557946618654029 1104 18-19 -0.11533889935767405 1104 20-21 -0.048091481118909485 1104 22-23 -0.0766122262193818 1104 24-25 -0.0808866264520347 1104 26-27 0.02023908541681152 1104 28-29 -0.13568099359966368 1104 30-31 -0.0948545601288302 1104 32-33 -0.06910337350706897 1104 34-35 0.13350713451463747 1104 36-37 0.14195904346506438 1104 38-39 -0.15247265943510513 1104 40-41 -0.07267475666476031 1104 42-43 0.06340478773449831 1104 44-45 0.24659066485384074 1104 46-47 0.15736251393102663 1104 48-49 0.165341054127488 1104 50-51 0.33491492407728884 1104 52-53 0.3757661838722868 1104 54-55 0.28760211171118755 1104 56-57 0.0384493817972924 1104 58-59 0.16638972480590297 1104 60-61 0.2578985573053387 1104 62-63 0.14759797177477196 1104 64-65 0.04671021155891708 1104 66-67 0.05623407550719861 1104 68-69 -0.1112507411341781 1104 70-71 -0.07197400909959839 1104 72-73 0.26019099797483136 1104 74-75 0.22013926672186912 1104 76-77 0.14654986206899423 1104 78-79 0.2762584576897744 1104 80-81 0.21315461782259604 1104 82-83 0.2103103204418808 1104 84-85 0.18424902236105112 1104 86-87 0.07197408489418322 1104 88-89 0.12244182582577068 1104 90-91 0.04125206007730853 1104 92-93 0.009302460515439037 1104 94-95 0.028382557423036303 1104 96-97 -0.0565439039284108 1104 98-99 -0.18817681623768578 1104 100-101 0.032857124055876596 1105 1 0.002985334584419519 1105 2 0.11116227250055744 1105 3 0.14650377972407824 1105 4 0.13274820361731798 1105 5 0.028687594413156603 1105 6 0.06302534147481254 1105 7 -0.008672850756070716 1105 8 0.1133058572918273 1105 9 0.13736764904136578 1105 10-11 -0.03733881152269447 1105 12-13 0.03438556993581443 1105 14-15 0.1290636812976942 1105 16-17 0.13820033827302325 1105 18-19 0.13264144998731098 1105 20-21 0.02923877354739801 1105 22-23 0.07462439317235692 1105 24-25 0.11511513186615474 1105 26-27 0.04126016591299475 1105 28-29 -0.008330450646468535 1105 30-31 0.03531170721282706 1105 32-33 0.12891948462949898 1105 34-35 0.11561334852707716 1105 36-37 0.08590057455444366 1105 38-39 0.34211230018154026 1105 40-41 0.30660510576132793 1105 42-43 0.5282495340342948 1105 44-45 0.35146613341665756 1105 46-47 0.14867333048626818 1105 48-49 0.14274183474348234 1105 50-51 0.17583253431032375 1105 52-53 0.29225041697504395 1105 54-55 0.31264220523053154 1105 56-57 0.31470000976807455 1105 58-59 0.14337073110493037 1105 60-61 0.30855647746609804 1105 62-63 0.3125572019792031 1105 64-65 0.19966070813889303 1105 66-67 0.3008958407813793 1105 68-69 0.24535213014146962 1105 70-71 0.3308851901893455 1105 72-73 0.3207235172003955 1105 74-75 0.3158352515732794 1105 76-77 0.33653710943151793 1105 78-79 0.5113480159847867 1105 80-81 0.439048268554906 1105 82-83 0.4128661615362894 1105 84-85 0.17381552929771615 1105 86-87 0.5167479188007427 1105 88-89 0.438888380681437 1105 90-91 0.582648377270182 1105 92-93 0.6118288352884029 1105 94-95 0.5016551547102068 1105 96-97 0.5605518761079118 1105 98-99 0.6924633275193166 1105 100-101 0.7890407040690128 1106 1 -0.07255604221172618 1106 2 -0.1265960445789709 1106 3 -0.031055898726926046 1106 4 0.014221759265879541 1106 5 0.028491166828800374 1106 6 -0.02940683342931294 1106 7 -0.03518991659517923 1106 8 -0.010765306743955705 1106 9 -0.03387081207189624 1106 10-11 -0.20617391369135873 1106 12-13 -0.1667342318072329 1106 14-15 -0.14851267869187268 1106 16-17 -0.10747331950472017 1106 18-19 -0.057864517199739396 1106 20-21 -0.1722195943334981 1106 22-23 -0.17722900143848364 1106 24-25 -0.12962853836186383 1106 26-27 -0.10513217992589574 1106 28-29 -0.054990392053795745 1106 30-31 0.08947521524496693 1106 32-33 0.13840608149033784 1106 34-35 -0.07787193486824151 1106 36-37 -0.08555833292651016 1106 38-39 0.13844387520832413 1106 40-41 -0.22970372709689713 1106 42-43 -0.14811924049447356 1106 44-45 0.08970531969976037 1106 46-47 0.1833738919335488 1106 48-49 0.10200630721202941 1106 50-51 0.19482053413133116 1106 52-53 0.13295504911257439 1106 54-55 0.15775872594196017 1106 56-57 0.33469005348884195 1106 58-59 0.2718184135884627 1106 60-61 0.29160382276539565 1106 62-63 0.04081197743977327 1106 64-65 0.21539136610878273 1106 66-67 0.21022411108678796 1106 68-69 0.3532190055412343 1106 70-71 0.20464920992028013 1106 72-73 0.30317269608703157 1106 74-75 0.2864306673417083 1106 76-77 0.18902427089827967 1106 78-79 0.2857966697484322 1106 80-81 0.3147635676644356 1106 82-83 0.1995216216308897 1106 84-85 0.26426483847802373 1106 86-87 0.318500006789769 1106 88-89 0.27857797245424365 1106 90-91 0.32931289211708403 1106 92-93 0.2268024738511194 1106 94-95 0.2769189664977034 1106 96-97 0.6248881614484247 1106 98-99 0.42051871417566744 1106 100-101 0.45273808977250596 1107 1 -0.12394664459014493 1107 2 -0.08379636230218068 1107 3 -0.17309338330552038 1107 4 -0.05374791056112116 1107 5 -0.2761344098895222 1107 6 -0.059122966895593265 1107 7 -0.019061520365042384 1107 8 -0.0028898272016135707 1107 9 -0.2016557066993343 1107 10-11 -0.246156900852867 1107 12-13 -0.08177003200214727 1107 14-15 -0.0228430094282146 1107 16-17 -0.08699876271170126 1107 18-19 -0.00839630923750434 1107 20-21 -0.08920736424330755 1107 22-23 -0.2274661671671936 1107 24-25 -0.06921324483406721 1107 26-27 -0.1832236436496757 1107 28-29 -0.19897984634728516 1107 30-31 -0.1778826684029795 1107 32-33 -0.17906503330124934 1107 34-35 -0.263699170056654 1107 36-37 -0.16081338466920414 1107 38-39 0.08732790060365403 1107 40-41 0.14031424341794008 1107 42-43 0.25426959863280985 1107 44-45 -0.009725426217435995 1107 46-47 0.006471355217932739 1107 48-49 -0.03715814834897202 1107 50-51 0.028363711250754875 1107 52-53 0.060681469149407974 1107 54-55 -0.11413826188428544 1107 56-57 -0.12681699106563826 1107 58-59 -0.0042726332644420495 1107 60-61 -0.08627272712108436 1107 62-63 0.02161760857664774 1107 64-65 -0.18774812226671855 1107 66-67 -0.08396701857694922 1107 68-69 -0.08507424622060711 1107 70-71 -0.004973465602077454 1107 72-73 -0.03529265574635687 1107 74-75 -0.03793994213478058 1107 76-77 -0.11993760795002828 1107 78-79 -0.15941861025923032 1107 80-81 -0.2185410353787205 1107 82-83 -0.2661837069929298 1107 84-85 -0.09919586580842221 1107 86-87 -0.03409388774189992 1107 88-89 -0.2994909577086169 1107 90-91 -0.10039225623825132 1107 92-93 -0.18705876448072445 1107 94-95 0.05850679386809077 1107 96-97 0.09444532064240008 1107 98-99 0.022828122500687442 1107 100-101 0.24270417676331846 1108 1 -0.2432266543000594 1108 2 -0.19127202682457423 1108 3 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sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 17.0 5 35.0 6 78.0 7 246.0 8 617.0 9 1084.0 10 1852.0 11 3159.0 12 4483.0 13 6336.0 14 7920.0 15 9968.0 16 12640.0 17 16084.0 18 14869.0 19 11983.0 20 11170.0 21 11573.0 22 12156.0 23 13604.0 24 15826.0 25 18741.0 26 23255.0 27 28901.0 28 35985.0 29 44950.0 30 54944.0 31 67916.0 32 83470.0 33 104684.0 34 135289.0 35 182150.0 36 259855.0 37 327881.0 38 277331.0 39 58472.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.22370449364266 19.89514457168161 13.988148883086303 17.89300205158943 2 14.54645776764582 28.18136203839496 34.0333203726022 23.23885982135703 3 29.060832329144432 24.43503615080048 27.73514159063522 18.768989929419867 4 12.31107028889723 16.60134634433848 43.948280071457994 27.139303295306295 5 11.99352102708499 43.67488118118646 32.401156406321086 11.930441385407468 6 27.507933460569856 41.74148399944287 15.02348175263736 15.727100787349915 7 29.196409461623595 32.38314133388974 21.91111417038709 16.509335034099575 8 23.852009675438303 30.472091695105384 18.81553318699 26.86036544246631 9 21.08769091943552 26.287916349834422 22.944749117261452 29.679643613468603 10-11 26.49757925434688 25.05373609005615 27.895846900676563 20.552837754920407 12-13 32.311054155996544 23.160956874605414 24.939394069760816 19.588594899637222 14-15 22.163416059917214 28.378719214153918 29.865544428902624 19.59232029702624 16-17 19.107189143203154 28.863076845308065 29.960624967061992 22.06910904442678 18-19 18.636699982899124 35.99422227050142 26.77617858905953 18.59289915753993 20-21 23.14054364975108 26.705813163323235 26.21515531985669 23.938487867068993 22-23 19.8162839046352 29.420844998316802 26.55284546724645 24.210025629801553 24-25 25.4609438607322 29.40672871021761 25.85700657254374 19.275320856506454 26-27 24.49884251571928 24.55098241629583 32.79798732992349 18.1521877380614 28-29 19.961115966386554 35.07248403361344 26.817156302521006 18.149243697478994 30-31 23.02149741904048 27.106528714317523 26.948465783866286 22.92350808277571 32-33 22.94508604229123 29.704643301683525 27.791188157229225 19.559082498796023 34-35 25.58466154018663 26.22284081066757 29.044161639813908 19.14833600933189 36-37 23.751862024489572 25.079046116352764 32.4537522734919 18.715339585665767 38-39 20.930775376342957 30.333507316573392 30.37932917763474 18.35638812944891 40-41 20.30968019523083 30.49290405856925 27.87299239648531 21.324423349714618 42-43 19.35614668893724 31.506183740832476 24.87714796128534 24.26052160894494 44-45 23.274263613748243 26.40840222978198 25.803068907920935 24.514265248548845 46-47 25.63489031103434 30.457096286823692 26.373374038445252 17.534639363696716 48-49 24.89551257989819 29.514281381449532 26.237151489012955 19.353054549639324 50-51 18.65511837784759 30.609409568100737 32.391826212091715 18.343645841959955 52-53 23.353422379774933 31.97411852519475 25.032050696027113 19.6404083990032 54-55 19.516453945408415 30.11824340675237 26.539186277999992 23.82611636983922 56-57 24.360257418916646 26.16559928627395 28.182337861521447 21.291805433287962 58-59 29.1630726110375 25.830710837879234 25.288671680060578 19.7175448710227 60-61 21.540050962511685 29.548683826985606 31.227391973565748 17.683873236936957 62-63 19.465167473327487 27.84760053589744 30.684995678506716 22.002236312268362 64-65 19.264147522364386 35.521188262214395 26.042626706416012 19.172037509005207 66-67 22.436357915204848 26.948366908670867 25.55042620799273 25.06484896813156 68-69 19.96751858673586 30.0252483833237 25.673975880937338 24.333257149003106 70-71 25.050173483018455 29.700470221795833 24.894544265595727 20.354812029589986 72-73 24.00658975216638 24.99521390613018 31.61529592579726 19.382900415906178 74-75 20.082261660591733 34.91076354867887 26.08289407029858 18.924080720430815 76-77 22.49278590457712 27.379844844516484 26.12478583574341 24.002583415162988 78-79 22.479933360364903 30.185625155279165 26.48600148207581 20.848440002280118 80-81 24.76284635993362 27.083133912755425 27.261725123336028 20.892294603974932 82-83 22.850290983752018 25.856441921019773 31.143569909774065 20.149697185454148 84-85 20.312698720418638 30.474116501622568 28.4749269112182 20.738257866740593 86-87 20.003188963283094 29.95775564743618 26.154930376542353 23.884125012738373 88-89 19.672405318694697 30.754525009760403 24.292115206119316 25.28095446542558 90-91 22.808856317159922 25.006775818287615 25.833210544033676 26.35115732051879 92-93 24.098385957062824 29.198452962377004 26.329404792762134 20.37375628779804 94-95 23.234357774730448 29.05772902046194 26.29146836600253 21.416444838805088 96-97 17.6607576094494 30.659814099835632 32.158190182391245 19.521238108323725 98-99 22.172302428801554 32.39919474643843 24.571580529254973 20.85692229550504 100-101 17.69529543202623 30.937996179126632 25.606116624025795 25.760591764821346 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 211.0 1 151.0 2 167.0 3 261.0 4 249.0 5 227.0 6 287.5 7 480.0 8 735.5 9 903.0 10 976.5 11 1028.5 12 1160.5 13 1314.0 14 1473.0 15 1659.0 16 1824.5 17 2003.0 18 2209.0 19 2431.5 20 2745.0 21 3138.5 22 3676.5 23 4467.5 24 5496.5 25 6905.0 26 8845.0 27 11345.0 28 14904.5 29 19376.5 30 24074.0 31 28536.5 32 32499.0 33 36095.0 34 38946.0 35 41849.0 36 44767.0 37 47314.5 38 49630.5 39 50330.0 40 50548.0 41 51403.5 42 52824.0 43 54506.5 44 55474.5 45 57157.5 46 65168.0 47 112390.0 48 233549.0 49 232512.0 50 113071.5 51 62054.0 52 43549.0 53 34975.0 54 29747.5 55 25741.5 56 21695.5 57 17517.0 58 13578.5 59 10629.5 60 8318.0 61 6175.5 62 4434.5 63 3078.0 64 1993.5 65 1227.0 66 738.5 67 424.0 68 231.0 69 119.5 70 54.0 71 33.0 72 22.0 73 13.5 74 5.0 75 2.0 76 1.5 77 1.5 78 1.5 79 1.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05350745393787972 2 0.004893646541052317 3 0.03624525020515672 4 0.0 5 0.0 6 0.0013444083903989882 7 0.0 8 0.0 9 0.0 10-11 1.613290068478786E-4 12-13 0.0 14-15 0.002957698458877774 16-17 0.0 18-19 0.0 20-21 0.011481247654007359 22-23 0.0 24-25 0.0 26-27 0.007179140804730596 28-29 0.009625964075256756 30-31 0.009034424383481201 32-33 0.004866758373244337 34-35 0.006641377448571001 36-37 0.01906371097585765 38-39 0.009652852243064735 40-41 0.011642576660855239 42-43 0.0 44-45 0.0 46-47 2.6888167807979762E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 9.14197705471312E-4 58-59 1.0755267123191905E-4 60-61 0.00795889767116201 62-63 0.013067649554678165 64-65 0.04877513640367529 66-67 0.022935607140206735 68-69 0.0015595137328628262 70-71 3.226580136957572E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 2.6888167807979762E-5 86-87 8.335332020473727E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 2.151053424638381E-4 96-97 4.0332251711969647E-4 98-99 8.335332020473727E-4 100-101 0.001693954571902725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1859554.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.783543446675324 #Duplication Level Percentage of deduplicated Percentage of total 1 82.75990387967472 39.54561462677111 2 10.83978926246847 10.359270823519335 3 2.700594211772252 3.8713188255017785 4 1.1213208222848368 2.143227289172368 5 0.5894399611231363 1.4082764995766999 6 0.371984538896597 1.0664843625514218 7 0.2705792446922128 0.905046456616326 8 0.19087198428981467 0.7296431803252391 9 0.14659802467115296 0.6304475772963737 >10 0.8910726286530082 7.97446335993436 >50 0.07004212619907392 2.2801534974844504 >100 0.039534147868257825 3.449091147360897 >500 0.002605354114366536 0.7638460621823053 >1k 0.0036248405069447464 4.193413121843094 >5k 3.3982879752606995E-4 0.9800850799273669 >10k+ 0.0016991439876303496 19.69961808993678 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 131092 7.049647388567366 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 31955 1.7184228046079866 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 31263 1.6812095803617426 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 23138 1.2442768534820716 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 18221 0.9798586112583986 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 17951 0.9653390006420894 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 16546 0.8897832491016663 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14319 0.7700233496849245 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 13220 0.7109231568429849 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 12368 0.6651057188981875 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 12015 0.6461226724257537 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 11062 0.5948738245837443 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 10427 0.56072585146761 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10202 0.5486261759540191 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 10170 0.5469053332143083 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6868 0.36933587301041004 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 6064 0.32609969917517856 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 5175 0.27829253681259053 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4924 0.2647946765729847 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4538 0.24403701102522435 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 4109 0.22096696304597768 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3950 0.21241652568304015 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3838 0.20639357609405265 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3541 0.19042200441611268 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3437 0.1848292655120529 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3225 0.17342868236146947 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3220 0.17315980068338968 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3136 0.16864258849164906 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 2817 0.15148793743015798 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2597 0.1396571435946469 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 2436 0.1309991535604774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2376 0.12777257342351983 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2316 0.12454599328656227 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2272 0.12217983451946005 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 2254 0.12121186047837278 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2103 0.11309163380036288 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2092 0.11250009410858731 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.3776335615959525E-5 0.0 12-13 0.0 0.0 0.0 5.3776335615959525E-5 0.0 14-15 0.0 2.6888167807979762E-5 0.0 1.0755267123191905E-4 0.0 16-17 0.0 5.3776335615959525E-5 0.0 3.7643434931171663E-4 0.0 18-19 0.0 5.3776335615959525E-5 0.0 6.990923630074738E-4 0.0 20-21 0.0 5.3776335615959525E-5 0.0 0.0013444083903989882 0.0 22-23 0.0 5.3776335615959525E-5 0.0 0.0027963694520298952 0.0 24-25 0.0 5.3776335615959525E-5 0.0 0.004920534708860296 0.0 26-27 0.0 1.613290068478786E-4 0.0 0.007501798818426353 0.0 28-29 0.0 2.151053424638381E-4 0.0 0.015622025496436242 0.0 30-31 0.0 2.151053424638381E-4 0.0 0.03110961015383259 0.0 32-33 0.0 2.151053424638381E-4 0.0 0.058374212311124066 0.0 34-35 0.0 2.151053424638381E-4 0.0 0.08203579998214626 0.0 36-37 0.0 2.151053424638381E-4 0.0 0.1053478414716647 0.0 38-39 0.0 2.151053424638381E-4 0.0 0.13371485850908338 0.0 40-41 0.0 2.151053424638381E-4 0.0 0.16017281563213545 0.0 42-43 0.0 2.151053424638381E-4 0.0 0.2435799121724887 0.0 44-45 0.0 2.151053424638381E-4 0.0 0.3267181270347621 0.0 46-47 0.0 2.151053424638381E-4 0.0 0.39063130191433 0.0 48-49 0.0 2.151053424638381E-4 0.0 0.462395821793828 0.0 50-51 0.0 2.151053424638381E-4 0.0 0.54209235117668 0.0 52-53 0.0 2.151053424638381E-4 0.0 0.7282122487435159 0.0 54-55 0.0 2.151053424638381E-4 0.0 0.8445035745130285 0.0 56-57 0.0 2.151053424638381E-4 0.0 1.1684253320957605 0.0 58-59 0.0 2.151053424638381E-4 0.0 1.506651594952338 0.0 60-61 0.0 2.151053424638381E-4 0.0 1.7371907457379565 0.0 62-63 0.0 2.151053424638381E-4 0.0 2.2076799060419865 0.0 64-65 0.0 2.151053424638381E-4 0.0 2.3866475509719 0.0 66-67 0.0 2.151053424638381E-4 0.0 3.1159891027633506 0.0 68-69 0.0 2.151053424638381E-4 0.0 3.8017987108736824 0.0 70-71 0.0 2.151053424638381E-4 0.0 4.045970162737946 0.0 72-73 0.0 2.151053424638381E-4 0.0 4.593305706637183 0.0 74-75 0.0 2.151053424638381E-4 0.0 5.0690918359993855 0.0 76-77 0.0 2.151053424638381E-4 0.0 5.239831701580056 0.0 78-79 0.0 2.151053424638381E-4 0.0 5.482712521389537 0.0 80-81 0.0 2.151053424638381E-4 0.0 5.963096527446904 0.0 82-83 0.0 2.151053424638381E-4 0.0 6.283442158711175 0.0 84-85 0.0 2.151053424638381E-4 0.0 6.999258962095212 0.0 86-87 0.0 2.151053424638381E-4 0.0 8.562268156773076 0.0 88-89 0.0 2.151053424638381E-4 0.0 10.741258387763947 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 260 0.0 47.510986 2 TACCTGG 1560 0.0 39.28793 2 TACGCAG 1190 0.0 36.717358 4 GTACCCG 195 0.0 36.56954 1 AGTACGC 1225 0.0 35.29388 2 ATAACGC 380 0.0 35.008095 3 ACCTGGG 1725 0.0 34.70368 3 GAGTACG 1310 0.0 34.475864 1 GTACGCA 1270 0.0 34.043304 3 GTATAAC 510 0.0 32.625763 1 GTACCGG 60 0.003935163 31.693602 1 TACCCGG 240 0.0 31.67399 2 ACGCAGT 1425 0.0 30.32893 5 CGCAGTG 1410 0.0 30.314747 6 TAACGCA 435 0.0 28.386683 4 GTACCTG 2260 0.0 28.187672 1 GTACACG 145 7.8336416E-7 26.229187 1 GGCGTCG 190 1.1941665E-8 24.995697 8 GCGTCGG 195 1.5726982E-8 24.356747 9 CTAACGC 125 2.2530991E-4 22.805275 3 >>END_MODULE