##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003855_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1010911 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.569886963342967 31.0 31.0 34.0 30.0 34.0 2 31.766757904503958 31.0 31.0 34.0 30.0 34.0 3 31.82935688700588 31.0 31.0 34.0 30.0 34.0 4 35.50909031556685 37.0 35.0 37.0 33.0 37.0 5 35.410051923463094 37.0 35.0 37.0 33.0 37.0 6 35.48799745971703 37.0 35.0 37.0 33.0 37.0 7 35.431914382176075 37.0 35.0 37.0 33.0 37.0 8 35.44455941225291 37.0 35.0 37.0 33.0 37.0 9 36.98270668733449 39.0 37.0 39.0 33.0 39.0 10-11 36.893187431930215 39.0 37.0 39.0 33.0 39.0 12-13 36.84714579226065 39.0 37.0 39.0 32.5 39.0 14-15 37.81374027980702 39.0 37.0 41.0 32.5 41.0 16-17 37.93312517125642 40.0 37.0 41.0 33.0 41.0 18-19 37.99090177077903 40.0 37.0 41.0 33.0 41.0 20-21 37.91693086730682 40.0 37.0 41.0 32.5 41.0 22-23 37.813623058805376 39.5 37.0 41.0 32.0 41.0 24-25 37.6675810234531 39.5 36.5 41.0 32.0 41.0 26-27 37.35945053521032 39.0 36.0 41.0 31.5 41.0 28-29 37.2417982394098 39.0 36.0 40.0 31.0 41.0 30-31 37.093529005026156 39.0 36.0 40.0 31.0 41.0 32-33 36.85490067869476 39.0 35.5 40.0 30.0 41.0 34-35 36.74681945294888 39.0 35.0 40.0 30.0 41.0 36-37 36.531961270576744 38.5 35.0 40.0 30.0 41.0 38-39 36.31012077225394 38.0 35.0 40.0 30.0 41.0 40-41 36.590935799491746 39.0 35.0 40.0 30.0 41.0 42-43 36.48346986035368 39.0 35.0 40.5 30.0 41.0 44-45 36.34011104835143 39.0 35.0 41.0 30.0 41.0 46-47 36.05953936597781 39.0 35.0 40.0 29.5 41.0 48-49 35.772158478837405 39.0 35.0 40.0 28.0 41.0 50-51 35.46113653922056 38.0 34.0 40.0 27.0 41.0 52-53 35.371870026144734 38.0 34.0 40.0 27.0 41.0 54-55 35.20506998143259 38.0 34.0 40.0 27.0 41.0 56-57 34.88168740868385 38.0 34.0 40.0 26.0 41.0 58-59 34.604450342315 38.0 34.0 40.0 26.0 41.0 60-61 34.04902162504909 37.0 33.0 40.0 23.5 41.0 62-63 33.95121924679819 37.0 33.0 40.0 24.0 41.0 64-65 33.50704315216671 36.5 32.0 39.5 22.5 41.0 66-67 33.12220215231608 36.0 32.0 39.0 22.0 40.5 68-69 32.77760752430234 36.0 31.5 39.0 22.0 40.0 70-71 32.25541318671971 35.0 31.0 38.5 20.5 40.0 72-73 31.821289411234027 35.0 31.0 37.5 20.5 40.0 74-75 31.48741481693245 35.0 31.0 37.0 19.5 39.0 76-77 30.540997674374893 34.0 29.5 36.0 18.0 39.0 78-79 30.932519282112867 34.0 30.0 36.0 18.0 39.0 80-81 30.643270277996777 34.0 30.0 36.0 18.0 38.5 82-83 30.30152901689664 34.0 30.0 35.0 14.0 37.0 84-85 29.967612875910937 34.0 30.0 35.0 10.5 37.0 86-87 29.73306700589864 34.0 30.0 35.0 7.0 36.0 88-89 29.4122385650171 34.0 29.0 35.0 4.5 36.0 90-91 29.05808028599946 34.0 29.0 35.0 2.0 36.0 92-93 28.746258078109747 34.0 29.0 35.0 2.0 35.0 94-95 28.32192547118391 34.0 29.0 35.0 2.0 35.0 96-97 27.833396312830708 34.0 27.5 35.0 2.0 35.0 98-99 27.54007177684287 34.0 27.0 35.0 2.0 35.0 100-101 26.53053582362839 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.019634131925865717 1101 2 0.052000991227195925 1101 3 0.05963067415817136 1101 4 0.002719411375750269 1101 5 0.1201337225633452 1101 6 0.254863197363143 1101 7 0.03248734539561582 1101 8 0.07710521573560669 1101 9 0.08401398933804671 1101 10-11 0.13218303127064956 1101 12-13 0.19811417948863408 1101 14-15 0.15995203326495755 1101 16-17 0.11335058975411272 1101 18-19 -0.02303460715353367 1101 20-21 0.04357703204571095 1101 22-23 -0.01967175108240582 1101 24-25 -0.04205128998857077 1101 26-27 0.0778081541293929 1101 28-29 -0.0660587989471324 1101 30-31 -0.118066554997867 1101 32-33 0.06743869289607574 1101 34-35 -0.0878400284470402 1101 36-37 -0.2556266984281024 1101 38-39 -0.1653740664324772 1101 40-41 0.0381212630580805 1101 42-43 -0.3108219614449652 1101 44-45 -0.23361149300554018 1101 46-47 -0.12379629267447712 1101 48-49 -0.1830247892076713 1101 50-51 -0.15838576053410947 1101 52-53 -0.2627141959979724 1101 54-55 -0.05267197899306808 1101 56-57 -0.10322551766166299 1101 58-59 -0.22800616633842452 1101 60-61 -0.15235056758797327 1101 62-63 -0.32581751167904827 1101 64-65 -0.42077129680786385 1101 66-67 -0.4344493109715515 1101 68-69 -0.5675400324774529 1101 70-71 -0.5147157107688258 1101 72-73 -0.4161669686049869 1101 74-75 -0.5880393354195128 1101 76-77 -0.4649917155800374 1101 78-79 -0.32301431137683423 1101 80-81 -0.24021647656610057 1101 82-83 -0.34546092727046585 1101 84-85 -0.4747984694125513 1101 86-87 -0.6730885668318578 1101 88-89 -0.40128915294238965 1101 90-91 -0.41171922092329893 1101 92-93 -0.3159502256030713 1101 94-95 -0.25384905156672843 1101 96-97 -0.17488726142503808 1101 98-99 -0.5293118973527591 1101 100-101 -0.511845166397201 1102 1 0.06204451537670863 1102 2 0.0816367376259457 1102 3 0.08116052516779249 1102 4 0.04819288459489002 1102 5 -0.04625063168228394 1102 6 -0.005188889278542774 1102 7 -0.07912839916810555 1102 8 -0.18332799267448507 1102 9 -0.14707637181012245 1102 10-11 -0.04423929399560933 1102 12-13 -0.03393780834370119 1102 14-15 0.11889851388774275 1102 16-17 -7.794998095036476E-4 1102 18-19 -0.14006369239071148 1102 20-21 -0.03881408358238758 1102 22-23 -0.2622681078406188 1102 24-25 -0.22203957402310692 1102 26-27 -0.19163082051189662 1102 28-29 -0.11130818668206643 1102 30-31 -0.0984476061881665 1102 32-33 -0.175768164472629 1102 34-35 -0.23406904028298925 1102 36-37 -0.20931863911426518 1102 38-39 -0.39207446943346014 1102 40-41 -0.17266201275410253 1102 42-43 -0.10811987868506634 1102 44-45 -0.2541673661625694 1102 46-47 -0.1078421119804105 1102 48-49 -0.10061073569534784 1102 50-51 -0.020444382708340925 1102 52-53 -0.21117806353698398 1102 54-55 -0.3173981006556019 1102 56-57 -0.106390945682584 1102 58-59 -0.11273800482748442 1102 60-61 -0.26775988530943096 1102 62-63 -0.46394945038901625 1102 64-65 -0.3866891297777144 1102 66-67 -0.4945218652637635 1102 68-69 -0.36989487095946316 1102 70-71 -0.49513910882228274 1102 72-73 -0.3850014417747225 1102 74-75 -0.47210597526650133 1102 76-77 -0.46766662576939666 1102 78-79 -0.2654031685055749 1102 80-81 -0.19111705318922034 1102 82-83 -0.4561820943500159 1102 84-85 -0.5395838766835084 1102 86-87 -0.737706351102819 1102 88-89 -0.6560090637316662 1102 90-91 -0.34089762119996436 1102 92-93 -0.22027454611236053 1102 94-95 -0.13847678735053393 1102 96-97 -0.264341480937059 1102 98-99 -0.5977514860235615 1102 100-101 -0.7154277110202081 1103 1 0.0666272665767842 1103 2 -0.06876082448553333 1103 3 -0.047319100393284685 1103 4 -0.10758597592823094 1103 5 -0.0014081296937931143 1103 6 -0.0441605154053093 1103 7 0.023426591594329693 1103 8 0.049705468840770095 1103 9 0.05458494868880592 1103 10-11 0.0265548663858155 1103 12-13 -0.07011992394883038 1103 14-15 0.07549659467207448 1103 16-17 -0.02096725073483441 1103 18-19 -0.019506033498728925 1103 20-21 0.09479957450248122 1103 22-23 -0.07664866850813468 1103 24-25 -0.1310704989236342 1103 26-27 0.1435666904349162 1103 28-29 0.022766899275481478 1103 30-31 0.24347224903743125 1103 32-33 0.14593511466038223 1103 34-35 -0.006955547492431435 1103 36-37 -0.031534015359262924 1103 38-39 -0.027361113202125864 1103 40-41 -0.018925009976975105 1103 42-43 0.23330747153440257 1103 44-45 0.30091775365554696 1103 46-47 0.4539115301245076 1103 48-49 0.22479004672778302 1103 50-51 0.2881299335271592 1103 52-53 0.2666587607943356 1103 54-55 0.2958998948242524 1103 56-57 0.4551406525558406 1103 58-59 0.3926297817870008 1103 60-61 0.3939477266065339 1103 62-63 0.3908669648230969 1103 64-65 0.401592719104535 1103 66-67 0.2909979470843993 1103 68-69 0.3293701323148994 1103 70-71 0.12037617256989819 1103 72-73 0.30325662634455597 1103 74-75 0.33922632396395613 1103 76-77 0.3310539871977838 1103 78-79 0.38890988722338093 1103 80-81 0.36619300922627573 1103 82-83 0.2932609944107796 1103 84-85 0.1759155508666872 1103 86-87 0.35285556850919164 1103 88-89 0.36002223319367843 1103 90-91 0.2504072220586835 1103 92-93 0.2731794385616588 1103 94-95 0.05463742066448063 1103 96-97 0.41787929101893795 1103 98-99 0.40587057639913837 1103 100-101 0.33577884742597064 1104 1 0.026489295643774113 1104 2 -0.07394568373908683 1104 3 -0.094961647100817 1104 4 -0.08198455568115293 1104 5 0.10417691248888872 1104 6 0.0771716079068483 1104 7 0.042187521762457436 1104 8 0.005505065704085155 1104 9 -0.03164994005137345 1104 10-11 0.029221903799715676 1104 12-13 -0.006908285337750897 1104 14-15 -0.0026147028311882536 1104 16-17 0.016697968591692813 1104 18-19 -0.015305589620588478 1104 20-21 -0.08800580051751439 1104 22-23 -0.0034894947040768898 1104 24-25 -0.00602377356890571 1104 26-27 0.05622516195816729 1104 28-29 0.10438858228456382 1104 30-31 0.12028206080173476 1104 32-33 0.20610413058112442 1104 34-35 0.3580827851228321 1104 36-37 0.3179186741368767 1104 38-39 0.2787312110061393 1104 40-41 0.15157262650944148 1104 42-43 0.3911958827297468 1104 44-45 0.46798139486185875 1104 46-47 0.19117014974295188 1104 48-49 0.1754228633576247 1104 50-51 0.368360550525928 1104 52-53 0.2894935817000004 1104 54-55 0.26797850342212115 1104 56-57 0.22523179118780945 1104 58-59 0.36528870146624826 1104 60-61 0.16919571474262796 1104 62-63 0.11841944236182655 1104 64-65 0.24435515263231622 1104 66-67 0.13131716655962578 1104 68-69 0.03669396881537068 1104 70-71 0.2001186560263264 1104 72-73 0.40778340926695833 1104 74-75 0.4289250001722742 1104 76-77 0.32063079553267215 1104 78-79 0.2772693918480478 1104 80-81 0.3581040482250124 1104 82-83 0.36926570412283155 1104 84-85 0.2955754921378819 1104 86-87 0.3522332428036776 1104 88-89 0.28439081705130675 1104 90-91 0.36672891046600853 1104 92-93 0.08823310014888364 1104 94-95 0.23032781006111236 1104 96-97 0.3053333168347585 1104 98-99 0.24957515604409863 1104 100-101 0.3651529262871662 1105 1 0.0989148910272526 1105 2 0.03215616241375585 1105 3 0.18255593026728434 1105 4 0.22170351645851127 1105 5 0.23449335396333737 1105 6 0.1789037805965492 1105 7 0.1904131764156176 1105 8 0.2629810240329391 1105 9 0.22070863500130145 1105 10-11 0.29662260266088936 1105 12-13 0.28267946472657 1105 14-15 0.20355683404464742 1105 16-17 0.310871628754974 1105 18-19 0.11340926785536709 1105 20-21 0.24040489277492583 1105 22-23 0.31326982154526917 1105 24-25 0.24289097253215886 1105 26-27 0.3113117705926527 1105 28-29 0.4141596124371958 1105 30-31 0.54580905618716 1105 32-33 0.6025599990936144 1105 34-35 0.5835280404546594 1105 36-37 0.6781679324517285 1105 38-39 0.6979362157118842 1105 40-41 0.7066465344981481 1105 42-43 0.7091972570047531 1105 44-45 0.5574932667090806 1105 46-47 0.4969662034243427 1105 48-49 0.4883052260685332 1105 50-51 0.5799487499977047 1105 52-53 0.684082455282983 1105 54-55 0.8146103898588066 1105 56-57 0.7735486299645089 1105 58-59 0.8433621763303663 1105 60-61 0.7563459177901279 1105 62-63 0.9152957348153663 1105 64-65 0.9425734799898251 1105 66-67 0.8728170151288523 1105 68-69 0.9374384220525727 1105 70-71 0.7692730209777565 1105 72-73 0.8347983341540406 1105 74-75 0.7006647606836864 1105 76-77 0.7390030937959366 1105 78-79 0.8672075494728304 1105 80-81 0.9826280182514893 1105 82-83 0.8855697510696281 1105 84-85 0.558280534294255 1105 86-87 0.7763110619399711 1105 88-89 0.4795531736398466 1105 90-91 0.659822690080496 1105 92-93 0.6596768379163684 1105 94-95 0.7719318690572408 1105 96-97 0.7070807954309153 1105 98-99 0.9207228710676603 1105 100-101 0.9208957349535645 1106 1 -0.038277741775484486 1106 2 -0.1537912671966417 1106 3 -0.08553994469665582 1106 4 -0.09327913199120985 1106 5 -0.12148528221355548 1106 6 -0.09626033797091793 1106 7 -0.0023218999399432505 1106 8 -0.0025597586662087224 1106 9 -0.2666382823668556 1106 10-11 -0.1720069308140495 1106 12-13 -0.12024582308188769 1106 14-15 -0.26711992012278074 1106 16-17 -0.08560299077840483 1106 18-19 0.003002708357463746 1106 20-21 -0.20747795585137396 1106 22-23 -0.14460481022599225 1106 24-25 -0.03679241630864993 1106 26-27 -0.17247567510180062 1106 28-29 -0.05412726069980067 1106 30-31 0.1492256070311413 1106 32-33 0.05008226764491752 1106 34-35 -0.17904025122621192 1106 36-37 -0.0754326234971856 1106 38-39 0.13269482374168007 1106 40-41 0.016095043663433728 1106 42-43 0.17274807475666165 1106 44-45 0.02295406619159479 1106 46-47 -0.11955492001204959 1106 48-49 -0.14910987371380457 1106 50-51 -0.13432453910447606 1106 52-53 -0.14029275778705852 1106 54-55 0.029617076980201773 1106 56-57 0.16814735897437316 1106 58-59 0.13084502146013222 1106 60-61 0.17101125577384835 1106 62-63 0.04023651226971481 1106 64-65 0.07354140803115428 1106 66-67 0.07375496039060891 1106 68-69 -0.01714160050007507 1106 70-71 0.0297062415478635 1106 72-73 -0.0972714391831424 1106 74-75 -0.11055823951736699 1106 76-77 -0.1525278636425007 1106 78-79 0.232177085919826 1106 80-81 -0.15201520415429925 1106 82-83 -0.1421053160201211 1106 84-85 -0.04136981229968484 1106 86-87 0.17508421555145048 1106 88-89 0.18862580539362384 1106 90-91 -0.07943552021854572 1106 92-93 0.015353460963776655 1106 94-95 0.21443704829831844 1106 96-97 0.18529738823329822 1106 98-99 0.2295564273050381 1106 100-101 0.0530232011027465 1107 1 -0.11592205654608989 1107 2 -0.20083592467667444 1107 3 -0.29571891985233023 1107 4 -0.19630246907161109 1107 5 -0.32835083823223243 1107 6 -0.07609142516815126 1107 7 -0.12670563345179175 1107 8 -0.14849124204227593 1107 9 -0.14327551980891684 1107 10-11 -0.10981716915288331 1107 12-13 -0.17923057798570596 1107 14-15 -0.09367800600222864 1107 16-17 -0.28016847817099944 1107 18-19 -0.2394115827097707 1107 20-21 -0.05400812817443068 1107 22-23 -0.16955228811197287 1107 24-25 -0.09552877003193316 1107 26-27 -0.2026258946631856 1107 28-29 -0.1633743104095089 1107 30-31 -0.15460692847128854 1107 32-33 -0.17040535650303923 1107 34-35 -0.3023914357630204 1107 36-37 -0.2933320336496408 1107 38-39 -0.23792079016710233 1107 40-41 -0.19139993568558822 1107 42-43 -0.1465979565082094 1107 44-45 -0.23173713745948277 1107 46-47 -0.3283192714955092 1107 48-49 -0.2450011497801441 1107 50-51 0.0031160445962044037 1107 52-53 0.0012264469923479737 1107 54-55 -0.014043640688704784 1107 56-57 -0.3109974673342606 1107 58-59 -0.09102459739141722 1107 60-61 0.17387102699215262 1107 62-63 -0.026312900619359425 1107 64-65 -0.08731203948251931 1107 66-67 -0.10774841407627633 1107 68-69 0.028855772341678687 1107 70-71 0.16934033397836146 1107 72-73 0.16362137360236062 1107 74-75 0.06576660186374639 1107 76-77 -0.0999057576215101 1107 78-79 -0.16671626576993148 1107 80-81 -0.05643337701625839 1107 82-83 0.1111796843377455 1107 84-85 0.1882876045806512 1107 86-87 0.26174675623240873 1107 88-89 0.345912169852884 1107 90-91 0.18572221453109705 1107 92-93 0.0768653821153471 1107 94-95 0.19671951833229429 1107 96-97 0.37612843753882785 1107 98-99 0.38157479215163903 1107 100-101 0.4059633869405097 1108 1 -0.21557637427700982 1108 2 -0.20869209007657474 1108 3 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-1.09521226363578 2316 52-53 -0.8847538826349748 2316 54-55 -0.9121585029091221 2316 56-57 -1.1593004084680985 2316 58-59 -1.2974946569678494 2316 60-61 -0.8389937156405196 2316 62-63 -0.6806300183371903 2316 64-65 -0.9328952653076712 2316 66-67 -1.2762620470795945 2316 68-69 -1.0941510985180507 2316 70-71 -1.3136745818753468 2316 72-73 -1.0898502949728766 2316 74-75 -1.161749089498393 2316 76-77 -1.2334327264707134 2316 78-79 -1.1576946385068254 2316 80-81 -1.2248546013048944 2316 82-83 -1.1680613320856388 2316 84-85 -0.882225680531036 2316 86-87 -0.7346927903488307 2316 88-89 -0.7751613657407752 2316 90-91 -0.878420305074048 2316 92-93 -1.033508673866212 2316 94-95 -1.2073711074377798 2316 96-97 -0.9327377256513394 2316 98-99 -1.3084356445973135 2316 100-101 -1.6896995640502581 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 4.0 5 19.0 6 45.0 7 140.0 8 365.0 9 660.0 10 1079.0 11 1853.0 12 2739.0 13 3850.0 14 4416.0 15 5660.0 16 7066.0 17 8925.0 18 8106.0 19 6377.0 20 6064.0 21 6279.0 22 6678.0 23 7430.0 24 8376.0 25 10047.0 26 12747.0 27 15759.0 28 20030.0 29 24204.0 30 30321.0 31 37309.0 32 45684.0 33 56674.0 34 73214.0 35 95940.0 36 134412.0 37 173972.0 38 158546.0 39 35907.0 40 11.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.28848664318382 20.62495113239237 14.066349097322501 19.020213127101307 2 14.671095845962693 25.846991508321526 37.38386264532008 22.0980500003957 3 25.373615793436947 26.520165802985762 28.419933959827702 19.686284443749585 4 12.45994949110258 17.63538036483924 42.046827069840965 27.857843074217215 5 12.029051024274144 41.04980557141034 33.55903734354458 13.362106060770929 6 26.88042592255522 41.50944516108609 16.752894484848966 14.857234431509722 7 27.70332897752621 33.85421664221677 21.93803410982767 16.504420270429346 8 23.79695146259166 32.594461827005546 19.5817435956281 24.026843114774692 9 22.681917597098064 23.646789875666602 22.495254280544973 31.176038246690364 10-11 25.103248660364088 26.127920515100765 28.276729161576363 20.492101662958785 12-13 29.68752936707584 24.160880631430462 26.40929814790817 19.742291853585527 14-15 21.965861427666983 27.895150759734094 29.217661839189617 20.921325973409306 16-17 19.129181500646446 29.87221427009895 29.565115029908668 21.43348919934594 18-19 19.160143672390546 33.809900179145345 28.284487951956205 18.745468196507904 20-21 22.217973515109843 27.729739355834443 27.476861585037078 22.57542554401864 22-23 20.048500807687322 29.29580348814089 27.71035234555762 22.945343358614164 24-25 23.744078361003098 29.300304378921588 27.736962007535777 19.21865525253954 26-27 23.265228191630914 26.42839265204449 31.696739045485977 18.609640110838622 28-29 19.822162952000024 32.50151115982877 28.96702950990935 18.709296378261854 30-31 21.891329596425866 28.641654580745495 27.87821348650301 21.588802336325628 32-33 22.008405701473446 29.64941290176745 28.27830518819838 20.063876208560718 34-35 23.75778248561721 27.09534166268072 29.8667400047584 19.280135846943665 36-37 23.237229910100307 26.669120455296525 31.04289974492471 19.050749889678457 38-39 20.843294290831764 29.56179701361714 30.985193726093087 18.60971496945801 40-41 20.229855719384886 30.095626469121733 28.908160600232684 20.766357211260694 42-43 19.47960799714317 31.247656816475438 26.659122316405696 22.613612869975693 44-45 22.392920840707045 27.503212448969293 27.12172486005197 22.982141850271688 46-47 23.995570325533592 30.1202036384068 26.947961217102023 18.936264818957582 48-49 23.527936682853387 29.554332676170308 27.63171040774114 19.28602023323517 50-51 19.89690487095303 29.87058207893672 31.100710151536585 19.131802898573664 52-53 21.982696795266843 32.04475962770214 26.493578564285087 19.47896501274593 54-55 19.77241319957939 30.144246130470435 27.28682346912834 22.796517200821835 56-57 22.9332536679149 27.51300951780824 28.351178090295274 21.202558723981582 58-59 25.954798040374616 27.248882810741833 26.639282031244203 20.157037117639348 60-61 21.28384477761955 29.288522045632053 30.432612666710522 18.995020510037875 62-63 19.87462300583256 28.750390164539375 30.14835403740534 21.226632792222727 64-65 19.81555189681358 33.11177984600622 27.522564854005804 19.550103403174397 66-67 21.49562348731771 27.86610621973399 26.944050297737434 23.69421999521087 68-69 20.15122220721904 29.960683413188193 26.688291036718397 23.199803342874368 70-71 23.535131713011047 29.60402014026966 26.53297524013018 20.32787290658911 72-73 22.7487879744112 26.896482479664378 30.148351338545133 20.206378207379284 74-75 20.11156273895526 32.42199362753002 27.539071194200083 19.927372439314638 76-77 21.610853972308146 28.77651939686085 26.773919761482468 22.838706869348538 78-79 21.527859524725717 29.764192891362345 27.073748331950092 21.634199251961846 80-81 22.88722746117116 28.06656570162952 27.838207319932216 21.207999517267098 82-83 22.362750034374933 27.401818755558104 29.399027214067313 20.836403995999646 84-85 20.196337758714662 29.589400055989103 28.84774228393993 21.366519901356302 86-87 20.304529110054855 29.62583032035651 26.781250463693425 23.288390105895214 88-89 19.73897801092282 30.401687191058365 25.947091286967893 23.912243511050924 90-91 21.777485851870242 26.390008615990922 26.801073487181366 25.03143204495747 92-93 22.72499755171326 29.424350907250986 26.47997697126651 21.37067456976925 94-95 22.31987896060037 29.656779705028292 26.780857454740058 21.242483879631283 96-97 19.153949564204098 29.97643704824074 29.64856381731689 21.22104957023827 98-99 21.293402855059863 31.435779948106006 25.408370898608872 21.862446298225258 100-101 18.676992763389904 29.960930911666466 25.766259126136426 25.595817198807204 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 156.0 1 104.0 2 129.0 3 192.0 4 166.5 5 152.5 6 202.5 7 322.0 8 448.0 9 542.5 10 585.0 11 616.5 12 671.0 13 773.0 14 881.0 15 957.0 16 1030.5 17 1166.0 18 1293.5 19 1389.0 20 1557.5 21 1776.5 22 2054.0 23 2418.0 24 3014.5 25 3861.0 26 4900.0 27 6399.5 28 8565.0 29 11466.5 30 14720.5 31 17663.5 32 20677.0 33 23379.0 34 25433.5 35 27152.5 36 28717.5 37 30472.5 38 32001.0 39 32376.0 40 32350.0 41 32413.0 42 32514.0 43 33264.5 44 33335.5 45 33610.5 46 36648.0 47 56784.5 48 102044.0 49 97546.5 50 51702.5 51 32646.0 52 24475.5 53 19411.5 54 15978.5 55 13641.5 56 11746.5 57 9816.0 58 7836.5 59 6342.0 60 5078.5 61 3804.0 62 2808.0 63 1935.0 64 1198.5 65 734.0 66 447.0 67 237.5 68 124.5 69 65.5 70 29.0 71 13.0 72 5.5 73 6.0 74 4.5 75 2.5 76 1.5 77 1.5 78 1.0 79 0.0 80 0.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05223011719132544 2 0.0038579063834501747 3 0.031061092420598846 4 0.0 5 0.0 6 0.002275175559470616 7 0.0 8 0.0 9 0.0 10-11 1.978413529974449E-4 12-13 0.0 14-15 0.0030665409714603956 16-17 0.0 18-19 0.0 20-21 0.014442418768813477 22-23 0.0 24-25 0.0 26-27 0.007122288707908016 28-29 0.0096942262968748 30-31 0.009199622914381186 32-33 0.004896573486686761 34-35 0.006677145663663764 36-37 0.022652834918207437 38-39 0.008853400546635658 40-41 0.01018882967936841 42-43 0.0 44-45 0.0 46-47 1.4838101474808367E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.00163219116222892 58-59 3.462223677455285E-4 60-61 0.006380383634167598 62-63 0.011821020841597332 64-65 0.053961229030053084 66-67 0.02858807550813078 68-69 0.0014343498092314754 70-71 9.892067649872245E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 5.935240589923347E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 4.946033824936122E-5 96-97 1.978413529974449E-4 98-99 3.956827059948898E-4 100-101 9.397464267378632E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1010911.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.77247824400103 #Duplication Level Percentage of deduplicated Percentage of total 1 86.3687289775686 51.6247297377374 2 8.978663772493306 10.733539700031125 3 1.989944784063006 3.5683179393650803 4 0.8225572048270902 1.9666513051989416 5 0.4401434022019782 1.3154230966179168 6 0.2806931928100013 1.0066636656284993 7 0.19134181935312353 0.8005882314116505 8 0.14708021083817072 0.7033078961958112 9 0.10535100573306432 0.5667381628346891 >10 0.6067270454657087 6.600438714450845 >50 0.039275057165185 1.5983843407872338 >100 0.020711747007592908 2.322275723692506 >500 0.003479573395001496 1.4902457446877995 >1k 0.0036452673661920435 4.811432745575727 >5k 0.0013255517695243794 5.188277635489802 >10k+ 3.3138794238109486E-4 5.702985360294913 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 45808 4.531358349053478 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11775 1.1647909657724567 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8356 0.8265811728233248 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 7415 0.7334968162380269 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7093 0.7016443584054383 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 7041 0.6965004832275047 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6175 0.610835177379611 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5854 0.5790816402235212 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5414 0.5355565425640832 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5038 0.4983623682005636 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 4762 0.47106026148691627 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3735 0.3694687267227283 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3228 0.31931594373787603 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3028 0.29953180843813154 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2788 0.2757908460784381 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2572 0.2544239799547141 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2518 0.2490822634237831 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2470 0.24433407095184445 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2466 0.24393838824584954 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2369 0.23434308262547343 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2327 0.23018841421252714 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2192 0.21683412288519957 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2128 0.21050319958928135 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1594 0.15767955833896355 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1425 0.14096196401067948 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1383 0.13680729559773314 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1352 0.13374075462627275 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1319 0.1304763723018149 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1298 0.12839903809534173 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1264 0.12503573509438515 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1251 0.12374976629990177 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1112 0.10999979226657935 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.892067649872245E-5 0.0 5 0.0 0.0 0.0 9.892067649872245E-5 0.0 6 0.0 0.0 0.0 9.892067649872245E-5 0.0 7 0.0 0.0 0.0 9.892067649872245E-5 0.0 8 0.0 0.0 0.0 9.892067649872245E-5 0.0 9 0.0 0.0 0.0 2.9676202949616734E-4 0.0 10-11 0.0 0.0 0.0 3.4622236774552856E-4 0.0 12-13 0.0 0.0 0.0 4.45143044244251E-4 0.0 14-15 0.0 0.0 0.0 6.429843972416959E-4 0.0 16-17 0.0 0.0 0.0 7.913654119897796E-4 0.0 18-19 0.0 0.0 0.0 9.892067649872245E-4 0.0 20-21 0.0 0.0 0.0 0.0013354291327327531 0.0 22-23 0.0 0.0 0.0 0.0023246358977199776 0.0 24-25 0.0 0.0 0.0 0.004698732133689315 0.0 26-27 0.0 0.0 0.0 0.0063309232959182365 0.0 28-29 0.0 0.0 0.0 0.012315624224090944 0.0 30-31 0.0 0.0 0.0 0.025026931154176778 0.0 32-33 0.0 0.0 0.0 0.04629487660140211 0.0 34-35 0.0 0.0 0.0 0.06766174272512615 0.0 36-37 0.0 0.0 0.0 0.08729749701012254 0.0 38-39 0.0 0.0 0.0 0.10802137873660492 0.0 40-41 0.0 0.0 0.0 0.13690621627423186 0.0 42-43 0.0 0.0 0.0 0.18903741278905858 0.0 44-45 0.0 0.0 0.0 0.26258493576585873 0.0 46-47 0.0 0.0 0.0 0.33919899971411926 0.0 48-49 0.0 0.0 0.0 0.48762947480045227 0.0 50-51 0.0 0.0 0.0 0.6134071149685778 0.0 52-53 0.0 0.0 0.0 0.7409653273136805 0.0 54-55 0.0 0.0 0.0 1.048707551901206 0.0 56-57 0.0 0.0 0.0 1.350217773869312 0.0 58-59 0.0 0.0 0.0 1.6982207137918175 0.0 60-61 0.0 0.0 0.0 2.0057156366880964 0.0 62-63 0.0 0.0 0.0 2.483997107559419 0.0 64-65 0.0 0.0 0.0 2.7778904374371236 0.0 66-67 0.0 0.0 0.0 3.350294931996981 0.0 68-69 0.0 0.0 0.0 4.021174960011317 0.0 70-71 0.0 0.0 0.0 4.2570513131225205 0.0 72-73 0.0 0.0 0.0 4.753089045425364 0.0 74-75 0.0 0.0 0.0 5.456563436346029 0.0 76-77 0.0 0.0 0.0 5.694319282310708 0.0 78-79 0.0 0.0 0.0 5.920550869463286 0.0 80-81 0.0 0.0 0.0 6.4288053053137215 0.0 82-83 0.0 0.0 0.0 7.198457628812031 0.0 84-85 0.0 0.0 0.0 8.245829751580505 0.0 86-87 0.0 0.0 0.0 9.62908703140039 0.0 88-89 0.0 0.0 0.0 10.747286358541949 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1285 0.0 45.47422 2 TATAACG 195 0.0 41.4169 2 TAACGCA 185 0.0 41.071415 4 GTACACG 95 1.915032E-8 40.030186 1 ACCTGGG 1380 0.0 39.58968 3 ATAACGC 195 0.0 38.98061 3 GTACCTG 1680 0.0 34.80303 1 GACGCAT 60 0.0039552837 31.659212 8 GTATAAC 370 0.0 29.54931 1 TACACGG 130 3.0320007E-7 29.235458 2 CCTGGGG 925 0.0 28.749989 4 AATGCGT 150 3.3434844E-8 28.493294 6 GTACCCG 85 6.37844E-4 27.962261 1 TATCACG 170 3.6343408E-9 27.94566 2 ATGCGTA 155 4.596768E-8 27.574156 7 CCCCGCA 90 8.9384237E-4 26.39312 2 TCACGCA 190 1.19362085E-8 24.994118 4 AGTACGC 465 0.0 24.520061 2 GAGTACG 490 0.0 24.252983 1 TACGCAG 470 0.0 24.24961 4 >>END_MODULE