##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003854_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2201601 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.894450901866414 31.0 31.0 34.0 28.0 34.0 2 30.792624094920015 31.0 31.0 34.0 27.0 34.0 3 30.886742874844263 31.0 31.0 34.0 27.0 34.0 4 34.30953655998521 37.0 35.0 37.0 30.0 37.0 5 34.28933489764948 37.0 35.0 37.0 30.0 37.0 6 34.41987898806369 37.0 35.0 37.0 30.0 37.0 7 34.472908578802425 37.0 35.0 37.0 30.0 37.0 8 34.45641876071095 37.0 35.0 37.0 30.0 37.0 9 35.90266401586845 39.0 35.0 39.0 30.0 39.0 10-11 35.724218421049045 39.0 35.0 39.0 30.0 39.0 12-13 35.59838272239157 39.0 35.0 39.0 30.0 39.0 14-15 36.53081007866548 39.0 36.0 41.0 30.0 41.0 16-17 36.65736638927763 39.0 36.0 41.0 30.0 41.0 18-19 36.67632986176878 39.0 36.0 41.0 30.0 41.0 20-21 36.53789151621933 39.0 36.0 41.0 30.0 41.0 22-23 36.44583827859817 39.0 36.0 41.0 30.0 41.0 24-25 36.18710361232576 39.0 36.0 41.0 28.0 41.0 26-27 35.99820630532054 39.0 35.5 40.5 27.5 41.0 28-29 35.86359676435467 39.0 35.0 40.0 27.0 41.0 30-31 35.67833976274538 38.5 35.0 40.0 27.0 41.0 32-33 35.41836145604948 38.0 34.5 40.0 27.0 41.0 34-35 35.35357587501096 38.0 35.0 40.0 26.0 41.0 36-37 35.19079683375871 38.0 34.5 40.0 25.0 41.0 38-39 35.19253193471478 38.0 34.5 40.0 25.5 41.0 40-41 35.423376215762985 39.0 35.0 40.0 26.0 41.0 42-43 35.26542002842477 39.0 35.0 40.0 25.5 41.0 44-45 35.031168454229444 38.0 34.5 40.0 24.5 41.0 46-47 34.75508368682608 38.0 34.0 40.0 24.0 41.0 48-49 34.45724815713656 38.0 34.0 40.0 23.0 41.0 50-51 33.6463557656451 37.5 32.5 39.5 22.0 40.5 52-53 33.83953563792895 38.0 33.0 39.5 23.0 40.5 54-55 34.21910623223736 38.0 34.0 40.0 22.5 41.0 56-57 33.876455588455855 38.0 33.0 40.0 20.5 41.0 58-59 33.55726673452638 38.0 32.5 40.0 19.0 41.0 60-61 33.11909083435191 37.5 32.0 40.0 17.0 41.0 62-63 32.978264908128224 37.0 32.0 40.0 16.5 41.0 64-65 32.702429050495525 36.5 31.5 39.5 13.5 41.0 66-67 32.15490795107742 36.0 31.0 39.0 12.0 41.0 68-69 31.72438443659864 36.0 30.5 39.0 8.0 40.0 70-71 31.164899089344527 35.0 30.0 38.5 7.5 40.0 72-73 30.749883607429318 35.0 29.5 37.0 6.5 40.0 74-75 30.426060625880893 35.0 29.0 37.0 2.0 39.0 76-77 30.018783149171895 34.0 29.0 36.5 2.0 39.0 78-79 29.399796784249283 34.0 29.0 36.0 2.0 38.5 80-81 28.818946076060104 34.0 27.5 35.5 2.0 37.5 82-83 28.387231610087387 34.0 26.5 35.0 2.0 37.0 84-85 28.094251410677956 34.0 26.0 35.0 2.0 36.5 86-87 28.06423757074965 34.0 26.5 35.0 2.0 36.0 88-89 27.65567284898581 34.0 26.0 35.0 2.0 36.0 90-91 27.18836860085002 33.0 25.0 35.0 2.0 35.5 92-93 26.90846570291347 33.0 25.0 35.0 2.0 35.0 94-95 26.65246858990344 33.0 25.0 35.0 2.0 35.0 96-97 26.360865342993577 33.0 24.5 35.0 2.0 35.0 98-99 26.148042038498346 33.0 24.0 35.0 2.0 35.0 100-101 25.883968303066723 32.0 22.5 35.0 2.0 35.0 102-103 25.358262464452004 32.0 19.5 35.0 2.0 35.0 104-105 24.93135177536711 32.0 18.5 34.5 2.0 35.0 106-107 24.413802500998138 31.0 16.0 34.0 2.0 35.0 108-109 24.184615877263866 31.0 10.0 34.0 2.0 35.0 110-111 23.883830903056456 31.0 4.5 34.0 2.0 35.0 112-113 23.18733071978074 31.0 2.0 34.0 2.0 35.0 114-115 22.617268297025664 31.0 2.0 34.0 2.0 35.0 116-117 21.928547679620422 30.0 2.0 34.0 2.0 35.0 118-119 21.188130138022284 29.5 2.0 34.0 2.0 35.0 120-121 19.59294463438198 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.44489186394445923 1101 2 0.2757267498210503 1101 3 0.36960914824003765 1101 4 0.5383406024179607 1101 5 0.49596127712686666 1101 6 0.6867908485087568 1101 7 0.4191774183957335 1101 8 0.6185536638915607 1101 9 0.6777404256861317 1101 10-11 0.6444393848595951 1101 12-13 0.8151607356525332 1101 14-15 0.9562606015599187 1101 16-17 0.7837875100029379 1101 18-19 0.7089456227778683 1101 20-21 0.7501103716408934 1101 22-23 0.7680352725550605 1101 24-25 0.739309323118448 1101 26-27 0.7397650142147754 1101 28-29 0.8313443200600545 1101 30-31 0.8099920005520147 1101 32-33 0.7050983843058134 1101 34-35 0.6148170874272054 1101 36-37 0.6426165752042152 1101 38-39 0.6248653062088465 1101 40-41 0.7098451858454595 1101 42-43 0.5929858513088178 1101 44-45 0.6633743172416189 1101 46-47 0.7550773429225828 1101 48-49 0.852865779301176 1101 50-51 0.6074902201478665 1101 52-53 0.5625192626129802 1101 54-55 0.762103972963466 1101 56-57 0.8091635037118508 1101 58-59 0.81188930277537 1101 60-61 0.6669263623385007 1101 62-63 0.6165855669915601 1101 64-65 0.7507553427021705 1101 66-67 0.7942887866874884 1101 68-69 0.696109044859373 1101 70-71 0.43304572584560574 1101 72-73 0.3435639150754781 1101 74-75 0.4448818164955277 1101 76-77 0.5080813130606394 1101 78-79 0.7721137951501795 1101 80-81 0.970047374795918 1101 82-83 0.842152144337458 1101 84-85 0.613065876551584 1101 86-87 0.6824401492666397 1101 88-89 0.718560028849069 1101 90-91 0.7616533195080457 1101 92-93 0.7034601734432577 1101 94-95 0.8851408015103175 1101 96-97 0.944562371439055 1101 98-99 0.8323104396815069 1101 100-101 0.7648031972759171 1101 102-103 0.5921017929238808 1101 104-105 0.6804990066759906 1101 106-107 0.7178273684617267 1101 108-109 0.7534161291945765 1101 110-111 0.5872959509132976 1101 112-113 0.7192437722272338 1101 114-115 0.6942804670818497 1101 116-117 0.7079453908515454 1101 118-119 0.643152176431947 1101 120-121 0.6482530125756654 1102 1 0.5246751480241763 1102 2 0.49477804935882475 1102 3 0.6161697301904816 1102 4 0.8982814980760665 1102 5 0.9244844263274459 1102 6 0.9372295845851397 1102 7 0.6775618997172472 1102 8 0.7037305980432649 1102 9 0.7780359473956153 1102 10-11 0.8685281929220352 1102 12-13 0.741137093915782 1102 14-15 0.9098462649682659 1102 16-17 0.8909133719412594 1102 18-19 0.7490160933393568 1102 20-21 1.0337377111877615 1102 22-23 0.7442632031453442 1102 24-25 0.8952705264222303 1102 26-27 0.9891959454870332 1102 28-29 1.0656877314414288 1102 30-31 0.9903398068716314 1102 32-33 0.7972140166464996 1102 34-35 0.8375805942848231 1102 36-37 0.8222528561771654 1102 38-39 0.7851305179994057 1102 40-41 0.7032883926348816 1102 42-43 0.8164858891962155 1102 44-45 0.771984607459089 1102 46-47 0.6699701215843987 1102 48-49 0.5094187837718849 1102 50-51 0.2676895078647732 1102 52-53 0.4048091769874631 1102 54-55 0.5190153922254197 1102 56-57 0.4727794770391185 1102 58-59 0.416348649596614 1102 60-61 0.6355586272471996 1102 62-63 0.47250888289668325 1102 64-65 0.5055571916072239 1102 66-67 0.3985056541573684 1102 68-69 0.23253853641048394 1102 70-71 0.04735655406413741 1102 72-73 0.06512108716004406 1102 74-75 0.08713838995919687 1102 76-77 -0.029230197131070668 1102 78-79 0.1678461585661104 1102 80-81 0.3624524668622975 1102 82-83 0.15237264899760916 1102 84-85 0.15518605538010277 1102 86-87 0.1854900848580634 1102 88-89 0.22297163754801375 1102 90-91 0.32223299670740957 1102 92-93 0.37431870189669425 1102 94-95 0.24763379234156702 1102 96-97 0.2609706460469212 1102 98-99 0.28936658880630617 1102 100-101 0.35606560539897814 1102 102-103 0.2685032477999876 1102 104-105 0.31229638486800937 1102 106-107 0.1090268835030237 1102 108-109 0.16347068704863332 1102 110-111 0.21338900236438718 1102 112-113 0.10077139214084951 1102 114-115 0.08781536137600554 1102 116-117 0.028462174969146048 1102 118-119 0.11864281824447787 1102 120-121 0.26498408782003935 1103 1 0.42075778224330307 1103 2 0.6393231308016816 1103 3 0.6121878946979109 1103 4 0.8013730533746255 1103 5 0.9437872894140469 1103 6 0.8868659455025352 1103 7 0.7382194243129376 1103 8 0.6576442252454981 1103 9 0.6560411415275382 1103 10-11 0.8475232795988958 1103 12-13 0.8455760329570126 1103 14-15 1.0320422894468209 1103 16-17 0.8500685694794399 1103 18-19 0.900586047130524 1103 20-21 0.91419956688528 1103 22-23 0.8587421366700951 1103 24-25 0.8795787979859426 1103 26-27 1.10123931964565 1103 28-29 1.1506852307477118 1103 30-31 1.0802182282941857 1103 32-33 1.0708519693676024 1103 34-35 0.9177396251123113 1103 36-37 0.8955297452809532 1103 38-39 0.6586004839201891 1103 40-41 0.9214774916041932 1103 42-43 0.9098236589762081 1103 44-45 0.8745501862824554 1103 46-47 0.7587734108689759 1103 48-49 0.8913790617892161 1103 50-51 0.8422526341805465 1103 52-53 0.86814177922097 1103 54-55 0.8681271890195532 1103 56-57 0.9855678253018496 1103 58-59 0.9113842325573387 1103 60-61 0.913294228319252 1103 62-63 0.8701230881662596 1103 64-65 0.7824511140076211 1103 66-67 0.847412493762377 1103 68-69 0.8639237379531046 1103 70-71 0.7132580583020243 1103 72-73 0.7871156437895408 1103 74-75 0.771797921756221 1103 76-77 0.8692236929015458 1103 78-79 0.9824262829527761 1103 80-81 0.806407434436359 1103 82-83 0.8962523465216741 1103 84-85 0.9473538992836446 1103 86-87 0.9383280814451531 1103 88-89 1.0199266067836668 1103 90-91 0.9348148092700335 1103 92-93 0.8636432321235183 1103 94-95 0.8857525063369778 1103 96-97 0.9742804844904747 1103 98-99 0.9172032976607554 1103 100-101 0.8156105289907352 1103 102-103 0.8830211456221413 1103 104-105 1.001543651136224 1103 106-107 1.0605798059914875 1103 108-109 1.23601556245427 1103 110-111 1.1673355290586684 1103 112-113 1.3219741178139195 1103 114-115 1.4541975637477584 1103 116-117 1.1870859127834557 1103 118-119 1.106807807535528 1103 120-121 1.211644789376784 1104 1 0.4348332474853436 1104 2 0.5330100252863339 1104 3 0.5618263899788047 1104 4 0.7358121561951521 1104 5 0.8107953308038276 1104 6 0.6854387408020131 1104 7 0.5950700965504794 1104 8 0.5995643367379486 1104 9 0.6486062012115568 1104 10-11 0.8385091793411448 1104 12-13 0.8026601943924874 1104 14-15 0.9063395052477006 1104 16-17 0.8066088781479763 1104 18-19 0.6598190160373818 1104 20-21 0.7714433147324158 1104 22-23 0.8118353190633272 1104 24-25 0.789317261599237 1104 26-27 0.8284673532600735 1104 28-29 0.738464175082548 1104 30-31 0.8970566871423955 1104 32-33 0.87614538171578 1104 34-35 0.763536265674162 1104 36-37 0.6591099317382287 1104 38-39 0.6338589634428899 1104 40-41 0.7774969992671075 1104 42-43 0.7183589999989692 1104 44-45 0.7719114717372833 1104 46-47 0.6044044163495172 1104 48-49 0.6461991393633966 1104 50-51 0.5382506303828976 1104 52-53 0.5161148331010779 1104 54-55 0.5051306991821107 1104 56-57 0.6508680318533706 1104 58-59 0.6606019057150121 1104 60-61 0.439314321507446 1104 62-63 0.5566194859549043 1104 64-65 0.4241898181610395 1104 66-67 0.3589296706653897 1104 68-69 0.349526761303256 1104 70-71 0.37814848506907595 1104 72-73 0.28080805944356513 1104 74-75 0.412133738971054 1104 76-77 0.5907922641547856 1104 78-79 0.5795828631404625 1104 80-81 0.547700471204358 1104 82-83 0.6492238873552019 1104 84-85 0.7355163011400236 1104 86-87 0.6747983591793165 1104 88-89 0.6577002673242376 1104 90-91 0.6990390735434424 1104 92-93 0.49459385264844613 1104 94-95 0.5488558501435357 1104 96-97 0.6649496730772704 1104 98-99 0.7390312056246202 1104 100-101 0.7312709742607382 1104 102-103 0.6018969159703325 1104 104-105 0.5573944993830153 1104 106-107 0.4076562500981744 1104 108-109 0.5324034516813256 1104 110-111 0.5142166222361375 1104 112-113 0.4631454954388694 1104 114-115 0.698989509733142 1104 116-117 0.6093552329639813 1104 118-119 0.556600684558866 1104 120-121 0.6094863638173535 1105 1 0.6159604577234319 1105 2 0.7275650701607965 1105 3 0.7097825530307418 1105 4 0.9650799036778821 1105 5 1.102388555713496 1105 6 0.8687601053579783 1105 7 0.7981127977407851 1105 8 0.8041598301800477 1105 9 1.0097902928072102 1105 10-11 1.0015150772868182 1105 12-13 1.100060480489681 1105 14-15 1.24675609427144 1105 16-17 1.095323431458688 1105 18-19 0.7210780425869885 1105 20-21 0.925302096947938 1105 22-23 1.0341333998892708 1105 24-25 1.148472759782436 1105 26-27 1.2918607929912298 1105 28-29 1.122815258865117 1105 30-31 1.1132804228686055 1105 32-33 1.1150400235284934 1105 34-35 1.135163720425325 1105 36-37 1.0915198163905018 1105 38-39 0.7823962303574987 1105 40-41 1.0038643981074102 1105 42-43 1.0280927283450438 1105 44-45 0.9286089522881085 1105 46-47 1.2824889850588903 1105 48-49 1.2490479602813878 1105 50-51 1.0435520596160046 1105 52-53 0.9957050017464866 1105 54-55 1.1296751581022235 1105 56-57 1.1733777522175117 1105 58-59 1.0463468830545537 1105 60-61 1.1850762109183322 1105 62-63 1.172849506035007 1105 64-65 1.3315497909235319 1105 66-67 1.1819350197244631 1105 68-69 0.9892066689312067 1105 70-71 0.7289664752650857 1105 72-73 0.8003373852020026 1105 74-75 0.8396123945409322 1105 76-77 1.0309152982806147 1105 78-79 0.9881779302010791 1105 80-81 1.2295291028399973 1105 82-83 1.2302176743963464 1105 84-85 1.0745833450535365 1105 86-87 1.0354566333167519 1105 88-89 1.119531766693516 1105 90-91 1.1492912531303894 1105 92-93 1.4350598414666713 1105 94-95 1.6349263411047055 1105 96-97 1.3800222384718346 1105 98-99 1.379073756268859 1105 100-101 1.314570283916506 1105 102-103 1.3407898673713952 1105 104-105 1.4949103221332116 1105 106-107 1.357899679318951 1105 108-109 1.4751665110559422 1105 110-111 1.425869246222362 1105 112-113 1.3025642444109486 1105 114-115 1.4767081341517105 1105 116-117 1.6871145664724558 1105 118-119 1.7230450786666047 1105 120-121 1.8401685881047527 1106 1 0.5125644574231529 1106 2 0.7595663090257325 1106 3 0.6018484681972645 1106 4 0.8439350054774266 1106 5 0.9260190952625535 1106 6 0.8603514669271846 1106 7 0.8046431353224932 1106 8 0.8961336396043222 1106 9 1.0358394958835149 1106 10-11 1.0307424678893327 1106 12-13 1.0299268350127306 1106 14-15 1.1122622690421977 1106 16-17 1.0363278830114737 1106 18-19 1.0182289678923269 1106 20-21 1.1430973399043651 1106 22-23 0.9300750383825473 1106 24-25 1.1079303877696418 1106 26-27 1.127274484426657 1106 28-29 1.1736851389388505 1106 30-31 1.2160964356923856 1106 32-33 1.3039365841499446 1106 34-35 1.2771816498037296 1106 36-37 1.2960002955021963 1106 38-39 1.2345744030496917 1106 40-41 1.2359336711553084 1106 42-43 1.188544729745736 1106 44-45 1.1752790532538029 1106 46-47 1.1983024711555927 1106 48-49 1.3171430526051537 1106 50-51 1.231484746456026 1106 52-53 1.2150512982101063 1106 54-55 1.292660404551384 1106 56-57 1.134900005261521 1106 58-59 1.3069629801175466 1106 60-61 1.2971496599659602 1106 62-63 1.4477018738807956 1106 64-65 1.261548303026963 1106 66-67 1.3389093438440725 1106 68-69 1.2507288770236578 1106 70-71 1.250457140849317 1106 72-73 1.0891352996295183 1106 74-75 1.1318063991718397 1106 76-77 1.3075457250012619 1106 78-79 1.4344943073572374 1106 80-81 1.2388225003102136 1106 82-83 1.2749303148193967 1106 84-85 1.3652134849832436 1106 86-87 1.3623560501601908 1106 88-89 1.5318388065120558 1106 90-91 1.5471812045242679 1106 92-93 1.3069443051743583 1106 94-95 1.3226560716605746 1106 96-97 1.4854321809473312 1106 98-99 1.4795116069191039 1106 100-101 1.503942993915576 1106 102-103 1.5704042234605566 1106 104-105 1.6400801423764264 1106 106-107 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68-69 -1.7975040218823892 2316 70-71 -1.5888269860665325 2316 72-73 -1.4839438292219 2316 74-75 -1.4541851130078989 2316 76-77 -1.5407359215211684 2316 78-79 -1.4326153891171636 2316 80-81 -1.624801024715957 2316 82-83 -1.6218795838142377 2316 84-85 -1.5025973169150468 2316 86-87 -1.342745244519147 2316 88-89 -1.3013705071501818 2316 90-91 -1.4844294740712272 2316 92-93 -1.3441761731257849 2316 94-95 -1.34449592053981 2316 96-97 -1.2420414021458477 2316 98-99 -1.1697894382871397 2316 100-101 -1.0329711385919147 2316 102-103 -1.1512024406253545 2316 104-105 -1.1741269202929345 2316 106-107 -1.2180695619804034 2316 108-109 -1.315169245817156 2316 110-111 -1.346839973239522 2316 112-113 -1.302141752976933 2316 114-115 -1.1437257283560562 2316 116-117 -1.0267077584960553 2316 118-119 -1.1029240462283383 2316 120-121 -1.5123731021338074 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 29906.0 3 4616.0 4 3991.0 5 4584.0 6 5381.0 7 5599.0 8 6900.0 9 8620.0 10 10993.0 11 13324.0 12 14560.0 13 16365.0 14 20188.0 15 20886.0 16 17765.0 17 17753.0 18 19310.0 19 20758.0 20 23100.0 21 24978.0 22 27982.0 23 30955.0 24 35092.0 25 38525.0 26 44431.0 27 50479.0 28 58962.0 29 68934.0 30 81720.0 31 96917.0 32 116472.0 33 141939.0 34 177688.0 35 225194.0 36 290477.0 37 311940.0 38 113792.0 39 525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.960635165371535 18.059333496032497 14.148121622946968 20.831909715649 2 13.542268041237113 22.082061855670105 37.32348224513173 27.05218785796105 3 24.489275998755524 23.79674868644788 31.120291453468845 20.59368386132775 4 10.73846072091519 15.216281886598146 44.17240981230404 29.872847580182622 5 10.529238263049532 38.94760762020999 36.649101443060545 13.874052673679929 6 26.188738343085898 36.482836449709346 18.484098303644945 18.844326903559814 7 23.661117755675377 33.194156305498076 24.532733933069515 18.611992005757028 8 21.77950893482847 28.21632297478901 21.84500749798237 28.159160592400152 9 20.264705195306647 21.010837468206933 28.227938142016136 30.496519194470284 10-11 24.51603181814851 22.91136150458366 28.59749529176407 23.975111385503762 12-13 28.840609680162267 22.200626883151354 27.52556116393152 21.43320227275486 14-15 23.06842334392039 24.420419941772337 31.78711475356526 20.724041960742014 16-17 19.217988477746328 28.666479294325526 30.845450116237277 21.270082111690865 18-19 17.791807445785242 32.319960443607165 29.52706922964475 20.361162880962844 20-21 20.592270312252992 27.080689985405744 26.580979808602134 25.746059893739133 22-23 18.54203988253163 26.895822066709407 29.498569668450315 25.06356838230865 24-25 23.676706498008738 25.851473955102932 27.8535692003529 22.618250346535422 26-27 21.65708513893597 23.41854403532721 34.11764436422937 20.806726461507452 28-29 18.469706678163824 30.479982644301884 30.193966420756098 20.856344256778193 30-31 19.496724366838837 26.467115942178403 29.05531862248142 24.98084106850134 32-33 20.995215582849408 26.08822351594638 30.88881476397273 22.02774613723149 34-35 24.314059736236747 24.6011703463382 29.113625324205184 21.971144593219876 36-37 21.900455184439636 23.888441431522413 33.723999894596105 20.487103489441854 38-39 21.2922974987273 27.330735544722273 31.379294702224968 19.99767225432546 40-41 19.848969207278515 29.3639595564851 30.52001554391798 20.267055692318408 42-43 18.72110025573596 28.885905837295844 26.74149956308146 25.65149434388674 44-45 20.252049884004272 25.966044991268532 27.397235482894782 26.38466964183241 46-47 23.560341950355852 27.677600529065987 28.80108690348646 19.9609706170917 48-49 22.248363144293233 26.210560774703918 29.48990763142094 22.051168449581912 50-51 17.32212228892844 27.754026147645998 34.01622993686168 20.907621626563877 52-53 19.9233277603083 29.872345806737755 27.76085812061834 22.4434683123356 54-55 18.30776259306644 26.246590771622024 30.26301921998321 25.18262741532833 56-57 22.522430629112016 24.222076694723622 28.66882298994218 24.586669686222184 58-59 25.632914800446265 24.78215430823381 27.83695479574439 21.747976095575535 60-61 22.51220317076189 25.680627552353318 32.91322342458784 18.893945852296945 62-63 19.73089567562131 27.70438344445012 31.397780383065953 21.16694049686262 64-65 18.319731581508336 31.716235323440984 28.820120573894346 21.14391252115633 66-67 20.135199622997085 27.255253378444166 26.35373164384588 26.25581535471287 68-69 18.632216358603262 27.587994956233665 28.964994492689268 24.81479419247381 70-71 23.42173205770791 26.226352705341938 27.153809472414725 23.198105764535423 72-73 21.388397911978895 24.018292527824126 33.004212662094 21.589096898102987 74-75 18.575177789959426 30.450575399626363 29.51998544313196 21.45426136728225 76-77 19.579897226207777 26.506057786192006 28.73602446034591 25.178020527254308 78-79 20.946054707939837 26.418339133828404 30.140615190311117 22.494990967920643 80-81 23.75365707989567 25.489032659363364 27.964058094933158 22.793252165807807 82-83 21.074376091019552 24.48082201737927 32.9381797053224 21.506622186278783 84-85 20.968202561122915 27.39564925405633 30.49508014109784 21.14106804372291 86-87 20.129487691290198 29.259282006060054 29.261734077235204 21.349496225414548 88-89 19.10388049112158 28.014637032664286 26.469741684056242 26.411740792157897 90-91 19.970296723357293 24.95352560245969 28.005881065421693 27.070296608761318 92-93 22.925542385632845 26.522693539083868 29.482362684592612 21.069401390690672 94-95 21.23996415199791 25.35714523046732 29.95941091402552 23.44347970350925 96-97 16.633165587306273 26.874343566383356 33.88342174394603 22.60906910236434 98-99 19.256420166919067 28.79259918470723 28.749569589423846 23.20141105894986 100-101 17.347824542221517 25.65028784143564 30.85804597898752 26.14384163735532 102-103 21.140095161468107 24.318675191767795 29.381212129684144 25.160017517079954 104-105 23.786360048256654 24.982327661763478 28.271066744726653 22.960245545253215 106-107 21.268049748414285 25.372062494171217 32.73017990572667 20.62970785168782 108-109 19.273127223358365 26.803851816208102 31.326539380295245 22.596481580138285 110-111 18.66286545982474 29.614653496563427 28.454860745130638 23.267620298481198 112-113 20.461393070487656 26.006730787739574 26.187581178726564 27.344294963046206 114-115 18.803959789773096 25.638534204955192 29.152597838606347 26.40490816666537 116-117 22.920415913378964 24.669258274359702 27.40633158229042 25.003994229970917 118-119 21.27652356646902 23.550355236774088 31.63126982133181 23.54185137542508 120-121 19.201708297401964 29.42717816283321 28.427692182884805 22.94342135688002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5157.0 1 5739.0 2 3854.5 3 1361.0 4 1244.0 5 1180.0 6 1234.5 7 1325.0 8 1480.0 9 1524.0 10 1436.0 11 1407.0 12 1484.0 13 1600.0 14 1739.5 15 1947.0 16 2126.0 17 2295.5 18 2501.0 19 2733.5 20 3044.5 21 3488.0 22 4118.5 23 4958.0 24 6064.0 25 7647.5 26 9752.0 27 12778.0 28 16934.5 29 21921.0 30 27275.0 31 32471.5 32 37169.0 33 40864.0 34 44361.0 35 48490.5 36 51856.0 37 55146.0 38 58466.0 39 60092.5 40 61318.5 41 62590.0 42 63569.5 43 65293.0 44 66367.0 45 69153.0 46 82206.5 47 143146.5 48 268596.5 49 262740.0 50 135672.0 51 73488.5 52 51153.0 53 40912.5 54 34459.5 55 29157.0 56 24075.0 57 19144.5 58 15260.0 59 12315.0 60 9875.5 61 7501.5 62 5411.5 63 3797.5 64 2513.0 65 1581.5 66 976.5 67 597.5 68 344.5 69 203.0 70 125.5 71 76.0 72 46.5 73 43.5 74 35.0 75 28.0 76 24.0 77 17.0 78 12.5 79 11.5 80 9.0 81 6.5 82 7.0 83 6.5 84 5.5 85 4.5 86 3.0 87 3.5 88 4.0 89 3.5 90 5.0 91 4.5 92 2.0 93 2.5 94 2.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42768875922567257 2 0.8675958995294788 3 0.8702757674982887 4 0.8497906750587413 5 0.8575123285281937 6 0.8674596350564884 7 0.8416602281703178 8 0.8333480953179073 9 0.8331209878629234 10-11 0.8263531857044032 12-13 0.8293737148556891 14-15 0.8300550372206408 16-17 0.8530837331560079 18-19 0.8811769253375158 20-21 0.8737278008140439 22-23 0.8500859147502204 24-25 0.8431591373732115 26-27 0.8439767242111537 28-29 0.8644845273961994 30-31 0.8720244949016647 32-33 0.8675050565474853 34-35 0.8784970573687058 36-37 0.8865593720206341 38-39 0.8735461148500567 40-41 0.8810406608645254 42-43 0.8753629744899281 44-45 0.8619863453913766 46-47 0.8578529897106696 48-49 0.8623951388103476 50-51 0.892327901377225 52-53 0.8659153043625979 54-55 0.8761351398368733 56-57 0.9204438043042313 58-59 0.8854692562367114 60-61 0.8989367283172564 62-63 0.8760670076003781 64-65 0.8647797670876786 66-67 0.8686633045679031 68-69 0.8857872066736888 70-71 0.8908289921743313 72-73 0.8960070421479641 74-75 0.8999360011191856 76-77 0.9134716054362257 78-79 0.9556681705722335 80-81 0.9803320401834845 82-83 0.9545099225518158 84-85 0.9146071427111453 86-87 0.8980737199883176 88-89 0.8965293892944272 90-91 0.9094972249740075 92-93 0.8964158355669352 94-95 0.8907381491923377 96-97 0.9007308772116291 98-99 0.933139111037831 100-101 0.9210797051781862 102-103 0.8961887281119513 104-105 0.8865593720206341 106-107 0.88760406631356 108-109 0.8863322645656502 110-111 0.8851285950542356 112-113 0.8942128932535913 114-115 0.8978920340243305 116-117 0.923396201219022 118-119 0.9192855562838135 120-121 0.8984143811707933 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2201601.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.561560222995126 #Duplication Level Percentage of deduplicated Percentage of total 1 83.71144636441372 43.16292783072744 2 10.517250919204773 10.845717333018555 3 2.5498177657553014 3.944177468599645 4 0.9768183064204226 2.0146510373368285 5 0.5314033341650983 1.369999250862706 6 0.32810801209481044 1.0150656615164269 7 0.23432511574192366 0.8457517996961249 8 0.18365882717592882 0.7575788542333045 9 0.1338219527289113 0.621006180731151 >10 0.750877968452743 7.060326143812998 >50 0.049544981833245 1.740955729824343 >100 0.024942177549681997 2.5317590943198964 >500 0.002870750368907646 1.0619998842618426 >1k 0.0032295941650211015 3.550407296333365 >5k 7.176875922269114E-4 2.844442653552571 >10k+ 0.001166242337368731 16.633233781172848 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 75999 3.45198789426422 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 63406 2.87999505814178 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 46248 2.1006531156190427 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 40228 1.8272157398184323 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 26573 1.2069852802574126 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 18449 0.8379810873995787 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 18416 0.836482178196685 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 13781 0.6259535674266136 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 11904 0.5406974288256592 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11829 0.5372908170009006 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 11115 0.5048598724292004 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 11091 0.5037697566452777 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 10549 0.47915130852502336 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9974 0.45303395120187534 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9595 0.4358192061140961 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 8551 0.3883991695134586 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8484 0.3853559296166744 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 7794 0.3540151008288968 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6264 0.2845202196038247 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5669 0.2574944324607411 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5162 0.23446573652537406 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4607 0.20925680902216162 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 4049 0.18391161704595882 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4005 0.18191307144210053 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3989 0.18118632758615208 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3791 0.1721928723687898 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3651 0.16583386362924074 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3063 0.13912602692313458 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2992 0.13590110106236325 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2795 0.12695306733599776 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2638 0.11982189324950343 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2618 0.11891346342956784 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2571 0.11677865335271924 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2454 0.11146433890609606 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2441 0.11087385952313793 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2256 0.10247088368873379 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.084298199355832E-5 0.0 5 0.0 0.0 0.0 9.084298199355832E-5 0.0 6 0.0 0.0 0.0 9.084298199355832E-5 0.0 7 0.0 0.0 0.0 9.084298199355832E-5 0.0 8 0.0 0.0 0.0 9.084298199355832E-5 0.0 9 0.0 0.0 0.0 9.084298199355832E-5 0.0 10-11 0.0 0.0 0.0 2.9523969147906453E-4 0.0 12-13 0.0 0.0 0.0 3.860826734726229E-4 0.0 14-15 0.0 0.0 0.0 4.5421490996779164E-4 0.0 16-17 0.0 0.0 0.0 4.7692565546618123E-4 0.0 18-19 0.0 0.0 0.0 7.267438559484666E-4 0.0 20-21 0.0 0.0 0.0 0.0011809587659162581 0.0 22-23 0.0 0.0 0.0 0.001907702621864725 0.0 24-25 0.0 0.0 0.0 0.0030205291512858143 0.0 26-27 0.0 0.0 0.0 0.0047692565546618125 0.0 28-29 0.0 0.0 0.0 0.01037881069276404 0.0 30-31 0.0 0.0 0.0 0.0265942829786142 0.0 32-33 0.0 0.0 0.0 0.05943402096928553 0.0 34-35 0.0 0.0 0.0 0.08425686579902535 0.0 36-37 0.0 0.0 0.0 0.09647524687715894 0.0 38-39 0.0 0.0 0.0 0.10748995844387789 0.0 40-41 4.542149099677916E-5 0.0 0.0 0.12325121581976026 0.0 42-43 4.542149099677916E-5 4.542149099677916E-5 0.0 0.14067035761702507 0.0 44-45 4.542149099677916E-5 4.542149099677916E-5 0.0 0.15697667288486877 0.0 46-47 4.542149099677916E-5 4.542149099677916E-5 0.0 0.17784784799788883 0.0 48-49 4.542149099677916E-5 4.542149099677916E-5 0.0 0.19887799832939757 0.0 50-51 4.542149099677916E-5 4.542149099677916E-5 0.0 0.228401967477304 0.0 52-53 4.542149099677916E-5 4.542149099677916E-5 0.0 0.3028250804755267 0.0 54-55 4.542149099677916E-5 4.542149099677916E-5 0.0 0.38610538421812124 0.0 56-57 4.542149099677916E-5 4.542149099677916E-5 0.0 0.4916422185491376 0.0 58-59 4.542149099677916E-5 4.542149099677916E-5 0.0 0.6542057348266103 0.0 60-61 4.542149099677916E-5 4.542149099677916E-5 0.0 0.8412514347513469 0.0 62-63 4.542149099677916E-5 4.542149099677916E-5 0.0 0.9295053917580888 0.0 64-65 4.542149099677916E-5 4.542149099677916E-5 0.0 1.0334297631587195 0.0 66-67 4.542149099677916E-5 4.542149099677916E-5 0.0 1.4165373289710534 0.0 68-69 4.542149099677916E-5 4.542149099677916E-5 0.0 1.7795458850173125 0.0 70-71 4.542149099677916E-5 4.542149099677916E-5 0.0 1.9821484456084457 0.0 72-73 4.542149099677916E-5 9.084298199355832E-5 0.0 2.117027563123382 0.0 74-75 4.542149099677916E-5 9.084298199355832E-5 0.0 2.6271563285082085 0.0 76-77 4.542149099677916E-5 9.084298199355832E-5 0.0 2.7569482390315048 0.0 78-79 4.542149099677916E-5 9.084298199355832E-5 0.0 2.8519245767057697 0.0 80-81 4.542149099677916E-5 9.084298199355832E-5 0.0 3.028091829536778 0.0 82-83 4.542149099677916E-5 9.084298199355832E-5 0.0 3.4186712306180818 0.0 84-85 4.542149099677916E-5 9.084298199355832E-5 0.0 3.7912182997736648 0.0 86-87 4.542149099677916E-5 9.084298199355832E-5 0.0 4.574193961576144 0.0 88-89 4.542149099677916E-5 9.084298199355832E-5 0.0 5.672440192387267 0.0 90-91 4.542149099677916E-5 9.084298199355832E-5 0.0 6.873588811051594 0.0 92-93 4.542149099677916E-5 9.084298199355832E-5 0.0 8.019709293373323 0.0 94-95 4.542149099677916E-5 9.084298199355832E-5 0.0 8.923687807191222 0.0 96-97 4.542149099677916E-5 9.084298199355832E-5 0.0 9.828688304556547 0.0 98-99 4.542149099677916E-5 9.084298199355832E-5 0.0 10.792691318726689 0.0 100-101 4.542149099677916E-5 9.084298199355832E-5 0.0 11.617977099392668 0.0 102-103 4.542149099677916E-5 1.8168596398711665E-4 0.0 12.249381245738896 0.0 104-105 4.542149099677916E-5 1.8168596398711665E-4 0.0 12.823894974611658 0.0 106-107 4.542149099677916E-5 1.8168596398711665E-4 0.0 13.687221253987438 0.0 108-109 4.542149099677916E-5 2.0439670948550623E-4 0.0 14.642162680703724 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2250 0.0 57.25273 2 ACCTGGG 2455 0.0 50.081226 3 GTACCTG 3425 0.0 38.69236 1 TATAACG 380 0.0 37.834423 2 GTACCCG 270 0.0 36.27798 1 GTACACG 275 0.0 35.61838 1 TACACGG 205 3.6379788E-12 33.66341 2 CCTGGGG 1770 0.0 32.45709 4 GTACCGG 165 1.2898454E-8 31.427982 1 ATAACGC 470 0.0 30.560106 3 AGTACGC 1225 0.0 30.51462 2 GTACGCA 1185 0.0 30.059797 3 GAGTACG 1370 0.0 28.598701 1 TACGCAG 1340 0.0 27.867075 4 GTATAAC 695 0.0 27.358173 1 TAACGCA 525 0.0 26.26242 4 TAGACCG 110 9.514705E-4 26.113802 5 TACCGCG 180 9.944633E-7 25.521805 8 TACCCGG 400 0.0 24.441036 2 CTGGGGG 1230 0.0 22.419655 5 >>END_MODULE