##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1003854_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2201601
Sequences flagged as poor quality	0
Sequence length	101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.613422686490424	31.0	31.0	34.0	30.0	34.0
2	31.794322858683294	31.0	31.0	34.0	30.0	34.0
3	31.867678112428184	31.0	31.0	34.0	30.0	34.0
4	35.50805164060154	37.0	35.0	37.0	33.0	37.0
5	35.42099181459311	37.0	35.0	37.0	33.0	37.0
6	35.52643871437195	37.0	35.0	37.0	33.0	37.0
7	35.48021689670381	37.0	35.0	37.0	33.0	37.0
8	35.50172124740132	37.0	35.0	37.0	33.0	37.0
9	37.03876088355701	39.0	37.0	39.0	33.0	39.0
10-11	36.9406472834996	39.0	37.0	39.0	33.0	39.0
12-13	36.912508215612185	39.0	37.0	39.0	33.0	39.0
14-15	37.84083196728199	39.0	37.0	41.0	32.5	41.0
16-17	37.985776260094354	39.5	37.0	41.0	33.0	41.0
18-19	38.05796054780135	40.0	37.0	41.0	33.0	41.0
20-21	37.97731264656947	40.0	37.0	41.0	32.5	41.0
22-23	37.86446794855199	39.5	37.0	41.0	32.5	41.0
24-25	37.676618288236604	39.5	36.5	41.0	32.0	41.0
26-27	37.37585738741943	39.0	36.0	41.0	31.5	41.0
28-29	37.301230104819176	39.0	36.0	40.0	31.5	41.0
30-31	37.18005942039452	39.0	36.0	40.0	31.0	41.0
32-33	36.926359726399106	39.0	35.5	40.0	31.0	41.0
34-35	36.81409233553219	39.0	35.0	40.0	30.0	41.0
36-37	36.60454551029001	38.5	35.0	40.0	30.0	41.0
38-39	36.385740422537964	38.0	35.0	40.0	30.0	41.0
40-41	36.71953773640183	39.0	35.0	40.0	30.0	41.0
42-43	36.598712255308754	39.0	35.0	40.5	30.0	41.0
44-45	36.43860377061965	39.0	35.0	40.5	30.0	41.0
46-47	36.18107027567666	39.0	35.0	40.0	29.5	41.0
48-49	35.88239331286641	39.0	35.0	40.0	28.0	41.0
50-51	35.561179114653385	38.0	34.5	40.0	28.0	41.0
52-53	35.5370239657413	38.0	34.5	40.0	28.0	41.0
54-55	35.373127782917976	38.0	34.0	40.0	27.0	41.0
56-57	34.99250295580353	38.0	34.0	40.0	26.5	41.0
58-59	34.71722578251009	38.0	34.0	40.0	26.0	41.0
60-61	34.04779589943863	37.0	33.0	40.0	23.5	41.0
62-63	33.98790698223702	37.0	33.0	40.0	25.0	41.0
64-65	33.58823079204633	36.5	32.5	39.5	23.5	41.0
66-67	33.152036404416606	36.0	32.0	39.0	22.5	40.0
68-69	32.7339202244185	35.5	31.5	39.0	22.0	40.0
70-71	32.13482188643628	35.0	31.0	37.5	21.5	40.0
72-73	31.668253239347184	35.0	30.5	37.0	20.5	39.5
74-75	31.369298297012037	34.0	30.5	37.0	20.0	39.0
76-77	30.47319314444352	34.0	29.5	35.5	20.0	38.5
78-79	30.848613122904652	34.0	30.0	36.0	19.5	38.5
80-81	30.51282339533821	34.0	30.0	35.5	18.0	37.0
82-83	30.17008122725235	34.0	29.5	35.0	14.5	37.0
84-85	29.827549360669803	34.0	29.0	35.0	11.0	36.5
86-87	29.695596068497427	34.0	29.5	35.0	7.5	36.0
88-89	29.368330819253806	34.0	29.0	35.0	6.5	36.0
90-91	28.943770692327995	34.0	29.0	35.0	2.0	35.5
92-93	28.673816236457014	34.0	29.0	35.0	2.0	35.0
94-95	28.232389293064458	34.0	29.0	35.0	2.0	35.0
96-97	27.72721873763684	33.5	27.0	35.0	2.0	35.0
98-99	27.49916061084638	34.0	27.0	35.0	2.0	35.0
100-101	26.414784513633485	33.0	25.5	34.5	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.07324563438055165
1101	2	0.08188622948294366
1101	3	0.10161084004749199
1101	4	0.10097341215632838
1101	5	0.1616033708808544
1101	6	0.06260320731604452
1101	7	0.0682820066201657
1101	8	-0.03635674536059241
1101	9	0.09931155858895124
1101	10-11	0.17350905688073937
1101	12-13	0.12923302448022156
1101	14-15	0.09711389421332939
1101	16-17	0.12243897645857515
1101	18-19	-0.012395749710456982
1101	20-21	-0.014084216238146041
1101	22-23	0.025309602717982216
1101	24-25	0.03800882588037524
1101	26-27	0.20657702490578345
1101	28-29	0.15664412564368035
1101	30-31	0.16385432988629844
1101	32-33	0.35068871302338067
1101	34-35	0.1564284478484197
1101	36-37	0.1739078044761584
1101	38-39	0.2645524191354909
1101	40-41	0.3169791660638239
1101	42-43	0.10882585922654897
1101	44-45	0.17380006401468506
1101	46-47	0.09921297683616359
1101	48-49	0.19249644732882842
1101	50-51	0.2603610316717919
1101	52-53	0.30773599386866124
1101	54-55	0.3433546619732013
1101	56-57	0.4639940411394363
1101	58-59	0.4246493470946433
1101	60-61	0.48955190112980773
1101	62-63	0.3286823393989877
1101	64-65	0.2590717611843445
1101	66-67	0.27603631465272827
1101	68-69	0.13382464047071352
1101	70-71	0.21255746426209754
1101	72-73	0.2574064470981128
1101	74-75	0.19134895015190523
1101	76-77	0.04018085457189002
1101	78-79	0.12265431981985486
1101	80-81	0.26593267829411005
1101	82-83	0.347494127448563
1101	84-85	0.3812611970483353
1101	86-87	0.13347401979474327
1101	88-89	0.2658391611964994
1101	90-91	0.261898879315968
1101	92-93	0.3124998789576452
1101	94-95	0.3945885225286503
1101	96-97	0.46336542020529237
1101	98-99	0.2549160126696677
1101	100-101	0.34067428158221347
1102	1	0.011590712807464598
1102	2	0.03291297798639192
1102	3	0.02949293446288692
1102	4	-0.011829497595883254
1102	5	0.0017837148984369833
1102	6	0.01108240713418951
1102	7	-0.12336609522116504
1102	8	-0.06672122213561238
1102	9	0.03523184350218855
1102	10-11	0.08454414061188942
1102	12-13	0.01024916451205371
1102	14-15	0.1095068623121449
1102	16-17	0.026914993735225323
1102	18-19	-0.055718474572167054
1102	20-21	-0.0048305979915710395
1102	22-23	-0.1122678770122647
1102	24-25	-0.019874784030967874
1102	26-27	-0.1591409412986593
1102	28-29	-0.08895108538912666
1102	30-31	-0.19490599442983125
1102	32-33	-0.04291513633632604
1102	34-35	-0.010051055068906578
1102	36-37	-0.15512379361431528
1102	38-39	-0.37033808628240905
1102	40-41	-0.0360828934951698
1102	42-43	0.030458048360443968
1102	44-45	0.033550007183592356
1102	46-47	0.07931981930035192
1102	48-49	-0.09511134232032958
1102	50-51	0.10320561756253
1102	52-53	0.10123754725200484
1102	54-55	0.03557941002200238
1102	56-57	0.0232900932725002
1102	58-59	-0.24547947006076498
1102	60-61	-0.0028387937250826667
1102	62-63	-0.19310443032140512
1102	64-65	-0.2191043394445913
1102	66-67	-0.2303166443530955
1102	68-69	-0.22651710386512036
1102	70-71	-0.1191868715718023
1102	72-73	-0.10829484865092098
1102	74-75	-0.06024459383536751
1102	76-77	-0.251817326832974
1102	78-79	-0.16956815498502564
1102	80-81	-0.10846680643726714
1102	82-83	-0.2753534894340639
1102	84-85	-0.20918284326385717
1102	86-87	-0.3227001106894569
1102	88-89	-0.13791093352199724
1102	90-91	-0.12388728420604522
1102	92-93	-0.05236183203727762
1102	94-95	-0.12122870866025437
1102	96-97	-0.053847582749927625
1102	98-99	-0.1532911556261567
1102	100-101	0.019674812005792575
1103	1	-0.018353806685482255
1103	2	-0.030406414602580867
1103	3	-0.021055805802269845
1103	4	-0.020261238627035993
1103	5	0.06834320897736035
1103	6	0.016081634494604202
1103	7	-0.02028201707988586
1103	8	0.022568165451971822
1103	9	-0.013509418608734336
1103	10-11	-0.018731518914918865
1103	12-13	0.010782036587862365
1103	14-15	0.03781654290460068
1103	16-17	-0.14075053806512727
1103	18-19	-0.057772366598690894
1103	20-21	-0.10963998863648783
1103	22-23	-0.13093219086677266
1103	24-25	-0.14849260658306918
1103	26-27	0.061842869825021296
1103	28-29	-0.14571151560814144
1103	30-31	0.11185294035772841
1103	32-33	0.0800700605538438
1103	34-35	0.04337788618224181
1103	36-37	-0.13905961966572278
1103	38-39	-0.020894220625322646
1103	40-41	0.18655918495516488
1103	42-43	0.11733937590479115
1103	44-45	0.23883710509945644
1103	46-47	0.2738744548886345
1103	48-49	0.4028930874799599
1103	50-51	0.5947066651953392
1103	52-53	0.5216938864690306
1103	54-55	0.5154779865374124
1103	56-57	0.5233116108695697
1103	58-59	0.3613990992433358
1103	60-61	0.5298955362302351
1103	62-63	0.4152772137559424
1103	64-65	0.33171623702762787
1103	66-67	0.260506225114014
1103	68-69	0.24118921998219633
1103	70-71	0.3572031735071448
1103	72-73	0.5991190020352306
1103	74-75	0.6278998748129112
1103	76-77	0.4777336449482341
1103	78-79	0.4817469108222667
1103	80-81	0.5018246697695865
1103	82-83	0.42832909360807747
1103	84-85	0.38986323762568986
1103	86-87	0.30623972810303357
1103	88-89	0.39251170863953533
1103	90-91	0.40154369083188257
1103	92-93	0.35292235809519923
1103	94-95	0.3698996442216895
1103	96-97	0.36256776835245574
1103	98-99	0.2479806257484114
1103	100-101	0.1403878669562566
1104	1	-0.08493717520015309
1104	2	-0.12405881057030044
1104	3	-0.103622783828893
1104	4	-0.08116677018816887
1104	5	-0.08701124394244886
1104	6	-0.13363876399316155
1104	7	0.009318659949116181
1104	8	-0.18451718285908925
1104	9	-0.21514305174390813
1104	10-11	-0.1373938450750103
1104	12-13	-0.15763648774423444
1104	14-15	-0.046395796346288876
1104	16-17	-0.050391853714067736
1104	18-19	-0.2491678351252986
1104	20-21	-0.1185643259335123
1104	22-23	-0.13022103260104245
1104	24-25	-0.0638296941943608
1104	26-27	-0.06774407461390552
1104	28-29	-0.17565779322344355
1104	30-31	-0.08621479102718155
1104	32-33	-0.028960550462016954
1104	34-35	-0.05587788128580229
1104	36-37	-0.13903640965433084
1104	38-39	-0.2195425539618796
1104	40-41	-0.2037921495589501
1104	42-43	-0.12333613389343867
1104	44-45	0.04396813528460086
1104	46-47	-0.14853746619521502
1104	48-49	-0.177298996464323
1104	50-51	0.05002809659894325
1104	52-53	0.0728289703178362
1104	54-55	-0.16604698506109372
1104	56-57	-0.19306952283750434
1104	58-59	0.01877422982163779
1104	60-61	-0.053291518350739864
1104	62-63	-0.0782449072311664
1104	64-65	-0.02927575182594211
1104	66-67	-0.05858492358989764
1104	68-69	0.04083688497592419
1104	70-71	-0.021360376471129428
1104	72-73	-0.10979255217218764
1104	74-75	-0.16859307488237718
1104	76-77	-0.15300448088906293
1104	78-79	-0.09835154182605521
1104	80-81	0.08461056040366444
1104	82-83	0.08736743250272738
1104	84-85	0.06732531431332944
1104	86-87	0.0030555255401196746
1104	88-89	0.047578410168107155
1104	90-91	0.04803508440140902
1104	92-93	-0.048741571138180007
1104	94-95	-0.09318325015816598
1104	96-97	0.05135008851311085
1104	98-99	-0.10753072333174174
1104	100-101	0.05456814649974362
1105	1	-0.02586862383719435
1105	2	-2.673803209596315E-4
1105	3	0.03867726185199416
1105	4	0.030401194005335697
1105	5	0.05440919106609243
1105	6	0.02425761615933908
1105	7	0.041180533032608935
1105	8	0.09612257232157617
1105	9	0.10274363141189724
1105	10-11	0.08193905485003228
1105	12-13	0.07652211781331886
1105	14-15	0.1495498606978245
1105	16-17	0.157418007192895
1105	18-19	0.09332298842534925
1105	20-21	0.07335815139060742
1105	22-23	0.1737629096815425
1105	24-25	0.14534303624404998
1105	26-27	0.08669471766850734
1105	28-29	0.16531295251259337
1105	30-31	0.1743899070046382
1105	32-33	0.2645450272003629
1105	34-35	0.4320238425562266
1105	36-37	0.1298870029646082
1105	38-39	0.2209937360936678
1105	40-41	0.2672865975715766
1105	42-43	0.34342550076247136
1105	44-45	0.3216966038651208
1105	46-47	0.2683039260596374
1105	48-49	0.2885552848810349
1105	50-51	0.2966542835413648
1105	52-53	0.30343989900811863
1105	54-55	0.29212228719282507
1105	56-57	0.3836843140489137
1105	58-59	0.2407058890640812
1105	60-61	0.5496446073570169
1105	62-63	0.4121469918389238
1105	64-65	0.4173798283919439
1105	66-67	0.3515069532662025
1105	68-69	0.28143158372567
1105	70-71	0.44005289516946533
1105	72-73	0.4113024925153326
1105	74-75	0.27034975360587055
1105	76-77	0.3412725807618422
1105	78-79	0.49715940100401923
1105	80-81	0.6111971559165958
1105	82-83	0.6543821118386006
1105	84-85	0.6004030905509516
1105	86-87	0.6555474607850229
1105	88-89	0.49780056008932405
1105	90-91	0.5486131057487214
1105	92-93	0.4375893625398142
1105	94-95	0.5558676035892063
1105	96-97	0.6537568170674035
1105	98-99	0.5600631506959992
1105	100-101	0.7320067790879712
1106	1	0.024471052610483213
1106	2	0.06197794100851084
1106	3	0.05625242198068392
1106	4	0.028076524185763674
1106	5	-0.05971067765737814
1106	6	-0.008202802870094672
1106	7	-0.03326621442998601
1106	8	0.0659454676783966
1106	9	0.005179646240520697
1106	10-11	0.003297540378099484
1106	12-13	0.032435315455877856
1106	14-15	0.048613722027660344
1106	16-17	0.028990731846171514
1106	18-19	-0.0016734761346128835
1106	20-21	-0.014517761631722692
1106	22-23	0.0871054086375409
1106	24-25	0.16255869628800923
1106	26-27	-0.0056294891500101585
1106	28-29	0.10071767611221105
1106	30-31	0.07424727571125089
1106	32-33	0.1746386523052763
1106	34-35	0.19246563995326227
1106	36-37	0.12815861556128993
1106	38-39	0.1764930422663724
1106	40-41	0.0771174583444676
1106	42-43	0.10398327317901845
1106	44-45	0.09707557921234411
1106	46-47	0.07060079334060987
1106	48-49	0.03149305798984159
1106	50-51	0.23758231744563574
1106	52-53	0.17721581189559998
1106	54-55	0.136988504613619
1106	56-57	0.2153256707408744
1106	58-59	0.17530800259643087
1106	60-61	0.22692579778215105
1106	62-63	0.024030970069787827
1106	64-65	0.028974430968482068
1106	66-67	0.23729617001676928
1106	68-69	0.2529812931812785
1106	70-71	0.0497193006675829
1106	72-73	0.10345995152962928
1106	74-75	0.24636281562958118
1106	76-77	0.030830266421890684
1106	78-79	0.20711610366340238
1106	80-81	0.20700892935894544
1106	82-83	0.19074028045818636
1106	84-85	0.1953077415537976
1106	86-87	0.10601185533024093
1106	88-89	0.08727337720795347
1106	90-91	0.047726548646981115
1106	92-93	-0.0048848925791560305
1106	94-95	0.003538371186508016
1106	96-97	0.1838004155834625
1106	98-99	0.2089399011658557
1106	100-101	0.29737466039069105
1107	1	-0.10048516312383171
1107	2	-0.10688537626979411
1107	3	-0.06907997856785641
1107	4	-0.038242843286340644
1107	5	-0.029331354770846474
1107	6	0.049335720086297385
1107	7	-0.06434800487777181
1107	8	-0.020278107017801972
1107	9	-0.1320879852634249
1107	10-11	0.0636730438371842
1107	12-13	0.01988350607410183
1107	14-15	0.020291659843927334
1107	16-17	-0.0472596268249319
1107	18-19	0.02063970540047677
1107	20-21	-0.009529714136469636
1107	22-23	-0.017085549299814318
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2309	2	0.03710340331220863
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2309	4	0.05519479252417625
2309	5	0.0301825776244371
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2310	96-97	0.9651103000633761
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2311	24-25	0.08516746978997247
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2311	88-89	-0.2221228696055526
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2314	82-83	0.25334884878718
2314	84-85	0.1093003272936599
2314	86-87	0.22248617718722485
2314	88-89	0.3747990287288552
2314	90-91	0.39766775813421873
2314	92-93	0.49247896301359617
2314	94-95	0.5926350605041577
2314	96-97	0.6102554807743772
2314	98-99	0.5700029445621269
2314	100-101	0.5896999471885565
2315	1	-0.5236822192322812
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2315	4	-0.29043369041389155
2315	5	-0.23851457661229603
2315	6	-0.20778058133380028
2315	7	-0.25129397030475076
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2315	9	-0.3970761616440086
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2315	12-13	-0.5092613996530773
2315	14-15	-0.5647846562450525
2315	16-17	-0.3847038281931887
2315	18-19	-0.32920032798386245
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2315	24-25	-0.4582776652735632
2315	26-27	-0.3391145846549506
2315	28-29	-0.4020009479357114
2315	30-31	-0.25639479285624844
2315	32-33	-0.3643242391546053
2315	34-35	-0.3862694632683983
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2315	64-65	-0.3433334858256316
2315	66-67	-0.2940344170993967
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2315	82-83	-0.5360099419236697
2315	84-85	-0.5554737517859678
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2315	90-91	-0.434127465965215
2315	92-93	-0.6853743103829899
2315	94-95	-0.7715671608135644
2315	96-97	-0.9052835114696762
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2315	100-101	-1.4207730554132496
2316	1	-0.4695817381905556
2316	2	-0.4234737630074328
2316	3	-0.3905753985707392
2316	4	-0.4072439913919652
2316	5	-0.3169059692111347
2316	6	-0.5046341906000293
2316	7	-0.3271483885736757
2316	8	-0.3713966402258819
2316	9	-0.5077700146768009
2316	10-11	-0.7444464486106739
2316	12-13	-0.7155825204366977
2316	14-15	-0.9126204529189295
2316	16-17	-0.8441873259914416
2316	18-19	-0.47358696345306583
2316	20-21	-0.587384694976528
2316	22-23	-0.7213429834080003
2316	24-25	-0.7323995097997127
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2316	28-29	-0.6839669698223858
2316	30-31	-0.7049281726951264
2316	32-33	-0.9158756350475912
2316	34-35	-0.7705048103886938
2316	36-37	-0.6682089701413148
2316	38-39	-0.5777408315920027
2316	40-41	-0.7064029798105835
2316	42-43	-0.9367181189955502
2316	44-45	-0.8928121578946389
2316	46-47	-0.8712526197094164
2316	48-49	-0.9754506098381626
2316	50-51	-0.8915135576457729
2316	52-53	-0.6316791943412312
2316	54-55	-0.859103800431761
2316	56-57	-0.7282503970469918
2316	58-59	-1.074595935924222
2316	60-61	-0.8938865669073621
2316	62-63	-0.8558928976234483
2316	64-65	-0.875074488698317
2316	66-67	-0.9096728752468266
2316	68-69	-0.9144359434023741
2316	70-71	-1.0663846177805212
2316	72-73	-0.8731333069617762
2316	74-75	-0.8832140837352682
2316	76-77	-1.004994213484057
2316	78-79	-1.0064679931436302
2316	80-81	-1.0723851664444588
2316	82-83	-0.9224373472533998
2316	84-85	-0.8042227042001429
2316	86-87	-0.8809510247936352
2316	88-89	-0.9174200146225182
2316	90-91	-1.1012504682856452
2316	92-93	-1.0218397436838629
2316	94-95	-1.354187409133793
2316	96-97	-1.1866054800106127
2316	98-99	-1.4235632889355117
2316	100-101	-1.7678604034830272
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	7.0
4	13.0
5	36.0
6	117.0
7	275.0
8	639.0
9	1253.0
10	2271.0
11	3645.0
12	5552.0
13	7887.0
14	9135.0
15	11576.0
16	14333.0
17	18044.0
18	16867.0
19	13501.0
20	13026.0
21	13330.0
22	14400.0
23	15878.0
24	18243.0
25	22203.0
26	27638.0
27	34407.0
28	43529.0
29	53516.0
30	66753.0
31	81647.0
32	101369.0
33	126728.0
34	162893.0
35	218149.0
36	305895.0
37	384602.0
38	325965.0
39	66241.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.7768260236343	19.815188026983364	13.90188651808699	18.506099431295343
2	14.16669808462499	26.987541681788922	36.11308829748431	22.732671936101777
3	28.35807670566595	25.248054731079407	27.86828334675316	18.52558521650148
4	12.51516510030655	16.892979245558116	41.945656819741636	28.6461988343937
5	11.603737461965178	41.34182351843045	33.42576606751178	13.628672952092591
6	26.748051159947057	41.28742903914759	16.256337534282412	15.708182266622941
7	27.415730643290953	34.09128175359658	21.35777554606852	17.13521205704394
8	23.925588696589436	29.650785950769464	19.196938954878746	27.226686397762357
9	21.680358975127646	24.97164563424526	23.41641378251554	29.93158160811155
10-11	25.524609248493473	25.332475468876826	27.736055425371926	21.406859857257775
12-13	31.596392806871	23.48990575494833	24.99394758632468	19.91975385185599
14-15	22.464938243341944	27.67850653904714	30.010801510212833	19.84575370739808
16-17	19.384507001949945	29.392814592653256	29.59030723550725	21.63237116988955
18-19	19.01548009834661	34.54635967189332	27.521880667750427	18.916279562009645
20-21	23.084989772227065	26.9682727805869	26.27533718943616	23.671400257749873
22-23	20.28698660656495	28.808694218434677	26.92551920170821	23.978799973292166
24-25	24.857773956316333	28.66670663757874	27.00219067851077	19.473328727594147
26-27	24.201216693940264	24.73688454126414	32.08678795752359	18.975110807272
28-29	19.490663496581515	33.76361000866262	28.166091505773792	18.57963498898208
30-31	22.295793505775254	27.681618775175544	26.632204023089667	23.390383695959535
32-33	22.34692788309762	29.13018198996591	27.88757185458064	20.635318272355832
34-35	25.305642713326453	26.223689425349228	29.223143198798073	19.247524662526246
36-37	23.970331724897232	25.50764773772929	31.090699577708865	19.431320959664617
38-39	20.55415931796475	29.410691836041707	31.391519531742418	18.643629314251125
40-41	21.115793126976364	30.083504025519108	27.6095689380652	21.191133909439337
42-43	19.440216460657496	31.569003647799942	25.39004569856209	23.60073419298047
44-45	23.29021016978099	25.55594769442783	26.592193590028344	24.561648545762836
46-47	25.599677507267444	30.164539425872093	26.092796148255815	18.14298691860465
48-49	24.416708567992114	28.978820412963113	27.455860530586605	19.148610488458175
50-51	18.6772035441481	29.534347958599223	31.972391909342342	19.816056587910342
52-53	22.344171355300073	32.82951815519706	25.141953514737686	19.684356974765183
54-55	20.01984010726739	29.009139258203465	26.70656490435824	24.26445573017091
56-57	23.57045605006433	26.566071673932324	27.90550369225563	21.957968583747718
58-59	28.38394466565997	26.217064555594494	25.163080370921403	20.235910407824136
60-61	22.13541140250363	29.00287430399484	30.96221613266997	17.899498160831563
62-63	20.210812319750946	28.432133910296876	30.03237413258426	21.32467963736792
64-65	19.684081161359128	34.21756557274433	26.523786272876137	19.574566993020404
66-67	22.684261409687466	27.339862760277533	25.62454795576054	24.35132787427446
68-69	20.593441483671842	29.58306931659978	26.01190516746042	23.811584032267955
70-71	24.846083005824195	29.181490216740833	25.768642471302023	20.203784306132945
72-73	24.10550322242768	25.508300550372205	30.50575467580184	19.880441551398278
74-75	19.743999876453515	33.89565908983033	26.937948097304666	19.422392936411487
76-77	22.33853909041647	28.03993548331419	25.757959775636003	23.863565650633333
78-79	22.17797411974286	29.64644819837927	26.668092901483963	21.507484780393902
80-81	24.839185138081397	27.429823764534884	27.2950808502907	20.435910247093023
82-83	23.27906448059037	26.41980232108414	29.746995424465066	20.554137773860425
84-85	20.229951258109455	29.796620362400567	29.77086633021393	20.20256204927605
86-87	21.233651838640835	30.163284596487394	25.87031028320272	22.73275328166905
88-89	19.839198837573203	31.266859889689368	24.450456735802717	24.44348453693471
90-91	23.121764570419437	25.047703920919368	26.28580292250957	25.54472858615162
92-93	24.53023958473856	29.51304527932173	26.04979285529031	19.9069222806494
94-95	23.252627579461013	28.625519139843163	27.199600653797685	20.92225262689814
96-97	18.093202333862422	29.489415928013727	31.401559738851958	21.01582199927189
98-99	21.438449045722255	32.23922839279765	25.18767715917589	21.1346454023042
100-101	18.68407121189169	29.850690677405062	25.905325312089712	25.55991279861354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	313.0
1	218.0
2	293.0
3	474.5
4	411.0
5	328.0
6	408.5
7	682.5
8	1004.0
9	1222.5
10	1313.0
11	1328.5
12	1396.0
13	1571.0
14	1796.0
15	2039.5
16	2256.5
17	2446.5
18	2657.5
19	2925.0
20	3238.5
21	3651.0
22	4150.0
23	4935.0
24	6114.5
25	7564.5
26	9506.0
27	12365.0
28	16531.0
29	21832.0
30	27254.5
31	32249.5
32	36659.5
33	40274.5
34	44211.5
35	48185.5
36	51286.5
37	54230.0
38	57443.5
39	59500.5
40	60350.0
41	61316.0
42	62445.5
43	63956.0
44	64564.0
45	66320.5
46	75841.0
47	145047.0
48	287744.0
49	279503.5
50	140474.5
51	74014.0
52	50276.5
53	39857.5
54	33762.5
55	28666.0
56	23748.0
57	18840.0
58	14981.5
59	12171.5
60	9569.5
61	7291.5
62	5403.5
63	3713.5
64	2327.0
65	1378.0
66	805.5
67	485.0
68	302.0
69	155.5
70	80.5
71	38.5
72	20.0
73	13.5
74	11.0
75	5.5
76	3.5
77	1.5
78	1.5
79	1.0
80	0.0
81	0.0
82	0.5
83	1.5
84	1.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04478559012282425
2	0.004542149099677916
3	0.03983464760417533
4	0.0
5	0.0
6	0.0015897521848872705
7	0.0
8	0.0
9	0.0
10-11	4.5421490996779164E-4
12-13	0.0
14-15	0.002589024986816412
16-17	0.0
18-19	0.0
20-21	0.010992000821220559
22-23	0.0
24-25	0.0
26-27	0.00651798395803781
28-29	0.008539240307394483
30-31	0.008334843597908976
32-33	0.004814678045658591
34-35	0.006063769048070018
36-37	0.017918778198229377
38-39	0.011287240512699621
40-41	0.012263802569130374
42-43	0.0
44-45	0.0
46-47	4.542149099677916E-5
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.001521619948392102
58-59	1.1355372749194791E-4
60-61	0.009016165962860664
62-63	0.013921686990512813
64-65	0.044172399994367735
66-67	0.02221110909742501
68-69	0.00163517367588405
70-71	3.860826734726228E-4
72-73	0.0
74-75	2.271074549838958E-5
76-77	0.0
78-79	0.0
80-81	4.542149099677916E-5
82-83	2.271074549838958E-5
84-85	1.8168596398711665E-4
86-87	7.04033110450077E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	1.1355372749194791E-4
96-97	6.586116194532978E-4
98-99	6.586116194532978E-4
100-101	0.0013853554754017646
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2201601.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.89994641666597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.68370504960721	39.126450949909334
2	11.760437517763972	11.266486538748849
3	2.8382443301463542	4.078552539942491
4	1.1504390014433308	2.2042386609911295
5	0.6082138551401572	1.4566705535543687
6	0.3851370213483899	1.1068825611397328
7	0.2641581951521852	0.8857214375319004
8	0.19821379738465392	0.7595544219015048
9	0.15528246026741466	0.6694219373631503
>10	0.854334007032843	7.514554329731123
>50	0.061871189490113	2.050306081509892
>100	0.030967835735672838	2.755153060539622
>500	0.0036929877897551033	1.1219156520760027
>1k	0.003030143827491367	3.4688443597925422
>5k	9.469199460910521E-4	2.9899607756155286
>10k+	0.0013256879245274731	18.54528613965293
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	126346	5.73882370147906	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	35631	1.6184131457062383	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	30177	1.3706843338098047	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	26316	1.1953119570712405	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	26055	1.183456947921081	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	25704	1.1675140045812116	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	24466	1.111282198727199	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	22275	1.0117637119532559	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	21455	0.9745180893358969	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	18590	0.8443855176301246	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	16319	0.7412333115764391	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	12411	0.5637261247610261	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	12360	0.5614096287201904	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	10765	0.48896235058032766	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	9611	0.43654594997004453	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	7927	0.36005615913146843	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	7328	0.3328486860243977	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	7031	0.3193585031983543	No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	6354	0.2886081537935348	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	6036	0.274164119656559	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	5750	0.26117357323148016	No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA	5424	0.24636616716653018	No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG	5423	0.2463207456755334	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5036	0.22874262865977985	No Hit
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	4594	0.20866632963920345	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4494	0.20412418053952558	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	4451	0.20217105642666408	No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA	4391	0.1994457669668573	No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA	4215	0.19145158455142416	No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA	3447	0.15656787946589779	No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG	3205	0.14557587864467722	No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA	3199	0.14530334969869654	No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA	3184	0.14462202733374485	No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT	2639	0.11986731474050022	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2634	0.11964020728551632	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC	2517	0.11432589283889316	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG	2499	0.11350830600095113	No Hit
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	2458	0.11164602487008317	No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT	2276	0.10337931350866937	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT	2252	0.10228919772474668	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.542149099677916E-5	0.0
3	0.0	0.0	0.0	4.542149099677916E-5	0.0
4	0.0	0.0	0.0	4.542149099677916E-5	0.0
5	0.0	0.0	0.0	4.542149099677916E-5	0.0
6	0.0	0.0	0.0	4.542149099677916E-5	0.0
7	0.0	0.0	0.0	4.542149099677916E-5	0.0
8	0.0	0.0	0.0	4.542149099677916E-5	0.0
9	0.0	0.0	0.0	4.542149099677916E-5	0.0
10-11	0.0	0.0	0.0	2.9523969147906453E-4	0.0
12-13	0.0	2.271074549838958E-5	0.0	4.0879341897101247E-4	0.0
14-15	0.0	4.542149099677916E-5	0.0	4.996364009645708E-4	0.0
16-17	0.0	4.542149099677916E-5	0.0	5.904793829581291E-4	0.0
18-19	0.0	4.542149099677916E-5	0.0	8.630083289388041E-4	0.0
20-21	0.0	6.813223649516874E-5	0.0	0.001294512493408206	0.0
22-23	0.0	9.084298199355832E-5	0.0	0.002021256349356673	0.0
24-25	0.0	9.084298199355832E-5	0.0	0.003043239896784204	0.0
26-27	0.0	9.084298199355832E-5	0.0	0.004837388791156981	0.0
28-29	0.0	9.084298199355832E-5	0.0	0.010560496656751156	0.0
30-31	0.0	9.084298199355832E-5	0.0	0.02752542354404817	0.0
32-33	0.0	9.084298199355832E-5	0.0	0.061001062408674415	0.0
34-35	0.0	9.084298199355832E-5	0.0	0.08727739495031116	0.0
36-37	0.0	9.084298199355832E-5	0.0	0.10001812317490771	0.0
38-39	0.0	9.084298199355832E-5	0.0	0.11155518188808963	0.0
40-41	0.0	9.084298199355832E-5	0.0	0.12756625746445427	0.0
42-43	0.0	9.084298199355832E-5	0.0	0.14523521746220136	0.0
44-45	0.0	9.084298199355832E-5	0.0	0.16210930136750484	0.0
46-47	0.0	9.084298199355832E-5	0.0	0.18325300542650552	0.0
48-49	0.0	9.084298199355832E-5	0.0	0.2044648417220014	0.0
50-51	0.0	9.084298199355832E-5	0.0	0.23398881086990786	0.0
52-53	0.0	9.084298199355832E-5	0.0	0.3085708990866192	0.0
54-55	0.0	9.084298199355832E-5	0.0	0.3927823433946478	0.0
56-57	0.0	9.084298199355832E-5	0.0	0.4989323678541207	0.0
58-59	0.0	9.084298199355832E-5	0.0	0.6631991900439725	0.0
60-61	0.0	9.084298199355832E-5	0.0	0.8530156009195127	0.0
62-63	0.0	9.084298199355832E-5	0.0	0.9421779877461902	0.0
64-65	0.0	9.084298199355832E-5	0.0	1.0476921113317084	0.0
66-67	0.0	9.084298199355832E-5	0.0	1.4394524711789285	0.0
68-69	0.0	9.084298199355832E-5	0.0	1.8124310444989806	0.0
70-71	0.0	9.084298199355832E-5	0.0	2.021483456811657	0.0
72-73	0.0	1.8168596398711665E-4	0.0	2.1614497813182316	0.0
74-75	0.0	1.8168596398711665E-4	0.0	2.683501688089713	0.0
76-77	0.0	1.8168596398711665E-4	0.0	2.8188804420056135	0.0
78-79	0.0	1.8168596398711665E-4	0.0	2.9187168792165337	0.0
80-81	0.0	1.8168596398711665E-4	0.0	3.10281018222648	0.0
82-83	0.0	1.8168596398711665E-4	0.0	3.504903931275467	0.0
84-85	0.0	1.8168596398711665E-4	0.0	3.889283298835711	0.0
86-87	0.0	1.8168596398711665E-4	0.0	4.69778583857838	0.0
88-89	0.0	1.8168596398711665E-4	0.0	5.832255708459435	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	2390	0.0	48.70301	2
TATAACG	265	0.0	48.4067	2
ACCTGGG	2580	0.0	43.273888	3
GTACCTG	3120	0.0	38.23705	1
GTACCCG	335	0.0	36.888737	1
AGTACGC	1455	0.0	33.95924	2
TCTAACG	100	1.2977143E-6	33.257195	2
ATAACGC	415	0.0	33.199203	3
GTACCGG	145	6.6029315E-10	32.779118	1
GTACGCA	1510	0.0	32.40694	3
TACGCAG	1520	0.0	32.182766	4
GAGTACG	1600	0.0	31.191376	1
TAACGCA	440	0.0	30.222828	4
TACCCGG	405	0.0	29.327333	2
ACGCAGT	1730	0.0	28.001663	5
CGCAGTG	1760	0.0	27.52436	6
CTAACGC	105	6.8995585E-5	27.148115	3
CCTGGGG	1755	0.0	26.520317	4
GTATAAC	610	0.0	25.7128	1
TACCGGG	205	2.6659109E-8	23.175747	2
>>END_MODULE