##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003853_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2526501 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.664764035319994 31.0 31.0 34.0 30.0 34.0 2 31.860903280861557 31.0 31.0 34.0 30.0 34.0 3 31.918305593387853 31.0 31.0 34.0 30.0 34.0 4 35.603543794362245 37.0 35.0 37.0 33.0 37.0 5 35.50433662998748 37.0 35.0 37.0 33.0 37.0 6 35.57862435043564 37.0 35.0 37.0 33.0 37.0 7 35.52283810693129 37.0 35.0 37.0 33.0 37.0 8 35.5330459002391 37.0 35.0 37.0 33.0 37.0 9 37.09670488948946 39.0 37.0 39.0 33.0 39.0 10-11 37.00477656648464 39.0 37.0 39.0 33.0 39.0 12-13 36.96621137296205 39.0 37.0 39.0 33.0 39.0 14-15 37.98184227910458 40.0 37.0 41.0 33.0 41.0 16-17 38.063820081606934 40.0 37.0 41.0 33.0 41.0 18-19 38.1118865577334 40.0 37.0 41.0 33.0 41.0 20-21 38.04876823717861 40.0 37.0 41.0 33.0 41.0 22-23 37.94515854139777 40.0 37.0 41.0 33.0 41.0 24-25 37.80003570154929 39.5 37.0 41.0 32.5 41.0 26-27 37.54021530171569 39.0 36.5 41.0 32.0 41.0 28-29 37.421556730038894 39.0 36.0 41.0 32.0 41.0 30-31 37.26649267108938 39.0 36.0 40.0 31.5 41.0 32-33 37.01730456469244 39.0 36.0 40.0 31.0 41.0 34-35 36.95506671083842 39.0 36.0 40.0 31.0 41.0 36-37 36.760007417372876 39.0 35.5 40.0 30.5 41.0 38-39 36.53324776044023 38.5 35.0 40.0 30.0 41.0 40-41 36.77418532587163 39.0 35.0 40.5 30.0 41.0 42-43 36.65366726551859 39.0 35.0 41.0 30.0 41.0 44-45 36.49859568628708 39.0 35.0 41.0 30.0 41.0 46-47 36.23135593455138 39.0 35.0 41.0 30.0 41.0 48-49 35.956467660214656 39.0 35.0 40.0 28.5 41.0 50-51 35.67346797012944 39.0 35.0 40.0 28.0 41.0 52-53 35.57842427135394 39.0 35.0 40.0 28.0 41.0 54-55 35.39765390949776 38.0 34.0 40.0 27.5 41.0 56-57 35.09370647389413 38.0 34.0 40.0 26.5 41.0 58-59 34.82629039133569 38.0 34.0 40.0 26.0 41.0 60-61 34.27468443511402 38.0 33.0 40.0 24.5 41.0 62-63 34.13636745047795 37.0 33.0 40.0 25.0 41.0 64-65 33.72153523786454 37.0 32.5 39.5 23.5 41.0 66-67 33.34148136098106 36.0 32.0 39.0 23.0 41.0 68-69 32.99012943196935 36.0 32.0 39.0 22.5 40.0 70-71 32.47391570397161 35.0 31.5 38.5 21.5 40.0 72-73 32.05767225106976 35.0 31.0 37.5 21.5 40.0 74-75 31.72379983225813 35.0 31.0 37.0 20.0 39.0 76-77 30.780740636952054 34.0 29.5 36.0 20.0 39.0 78-79 31.16114618597024 34.0 31.0 36.0 20.0 39.0 80-81 30.891088109602965 34.0 31.0 36.0 19.0 38.0 82-83 30.5593603564772 34.0 30.5 35.5 17.5 37.0 84-85 30.21395875164902 34.0 30.0 35.0 16.0 37.0 86-87 29.975916098984328 34.0 30.0 35.0 11.0 36.0 88-89 29.662713768963478 34.0 30.0 35.0 7.0 36.0 90-91 29.30575487601232 34.0 29.0 35.0 4.5 36.0 92-93 29.031362148679143 34.0 29.0 35.0 2.0 35.0 94-95 28.65827957321212 34.0 29.0 35.0 2.0 35.0 96-97 28.2241257771123 34.0 29.0 35.0 2.0 35.0 98-99 27.936428879307783 34.0 29.0 35.0 2.0 35.0 100-101 26.90279362644226 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.010155768976911617 1101 2 0.04227352121251471 1101 3 0.044104559918380914 1101 4 0.024494302633783605 1101 5 -0.012538695202941597 1101 6 0.05545535062946527 1101 7 7.620901832723348E-4 1101 8 -0.025546379921387086 1101 9 0.07677951665777982 1101 10-11 0.05774275389267558 1101 12-13 0.08539625186779887 1101 14-15 0.04327629516566134 1101 16-17 0.09600087321732786 1101 18-19 0.049799561261046676 1101 20-21 0.03581601827505665 1101 22-23 -0.05747327717092787 1101 24-25 0.010300764739135104 1101 26-27 0.1279592782198833 1101 28-29 0.009624001246997693 1101 30-31 0.040004844075589574 1101 32-33 0.09213718657075987 1101 34-35 -0.08193248361576622 1101 36-37 -0.08493266894668494 1101 38-39 -0.03227840554653483 1101 40-41 0.03571893038872531 1101 42-43 -0.09352274518187897 1101 44-45 -0.0661023599102819 1101 46-47 -0.03382508807170126 1101 48-49 0.018924333990817388 1101 50-51 -0.03907628647331052 1101 52-53 -0.0704258489826941 1101 54-55 0.0352838809863627 1101 56-57 0.01905390777042726 1101 58-59 -0.054465262409586046 1101 60-61 -0.05097714989077673 1101 62-63 -0.16894080471257666 1101 64-65 -0.26617840154000305 1101 66-67 -0.19343141493122573 1101 68-69 -0.1850294100222527 1101 70-71 -0.16732608612359456 1101 72-73 -0.10779890387521363 1101 74-75 -0.23340677439699675 1101 76-77 -0.19882853921894394 1101 78-79 -0.17652594862533277 1101 80-81 -0.025841940340630032 1101 82-83 -0.0744630877491268 1101 84-85 -0.19025651002753108 1101 86-87 -0.24172343313680145 1101 88-89 -0.2858456538299592 1101 90-91 -0.19809941481707583 1101 92-93 -0.05948386896184843 1101 94-95 -0.08802953032243366 1101 96-97 0.04223911644983147 1101 98-99 -0.20560536457892908 1101 100-101 -0.10733509843427314 1102 1 -0.10924873293981108 1102 2 0.00425471999720628 1102 3 -0.010536256228423468 1102 4 0.01412173189395105 1102 5 -0.08327528910584192 1102 6 -0.0609294042925157 1102 7 -0.12617460508180045 1102 8 -0.028490113554724417 1102 9 -0.04406724601227552 1102 10-11 -0.044623867215698 1102 12-13 -0.11689985173229189 1102 14-15 0.045547230900830016 1102 16-17 -0.027106788260638837 1102 18-19 -0.10477505433543399 1102 20-21 -0.1265570739533004 1102 22-23 -0.20359507323265547 1102 24-25 -0.12218537998428758 1102 26-27 -0.07861309601201327 1102 28-29 0.02992946055837109 1102 30-31 -0.13796962629842824 1102 32-33 -0.11677595019133236 1102 34-35 -0.23681565378247882 1102 36-37 -0.30215638147872426 1102 38-39 -0.10109902558257744 1102 40-41 -0.051610215207588794 1102 42-43 -0.037411681077635706 1102 44-45 0.01752844780119034 1102 46-47 -0.053838613100992916 1102 48-49 -0.09722158523199198 1102 50-51 0.05360836924497647 1102 52-53 0.17639244507639518 1102 54-55 0.08675712922957501 1102 56-57 0.18865196876559054 1102 58-59 -0.04950216376623473 1102 60-61 0.11947987781459801 1102 62-63 0.07932021669694223 1102 64-65 -0.08649808522785918 1102 66-67 0.05927607317771333 1102 68-69 0.1554655766944677 1102 70-71 0.1753107592852743 1102 72-73 0.15305187788270302 1102 74-75 0.2062285548962386 1102 76-77 0.1741832857923029 1102 78-79 0.15374076167138995 1102 80-81 0.3346417488971092 1102 82-83 0.10430656628122748 1102 84-85 0.15644011057143814 1102 86-87 0.0705628234821738 1102 88-89 0.06151459113150537 1102 90-91 0.15516254337232027 1102 92-93 0.21114287994574354 1102 94-95 0.24605952247655338 1102 96-97 0.4413194317259048 1102 98-99 0.24147175269468946 1102 100-101 0.07564283414002304 1103 1 -0.044768426948365914 1103 2 -0.028587120294460533 1103 3 -0.11465268307734178 1103 4 -0.00904953874009351 1103 5 -0.06899337086864676 1103 6 0.019050480732111907 1103 7 -0.05222706973773228 1103 8 5.668350644683073E-4 1103 9 -0.08545318876840469 1103 10-11 -0.042179616746160775 1103 12-13 -0.026380081530639643 1103 14-15 0.05057264129586514 1103 16-17 -0.10507386617950942 1103 18-19 0.005710876610770299 1103 20-21 0.05433674716818615 1103 22-23 -0.00968532966049196 1103 24-25 -0.11611957677580165 1103 26-27 0.09389436614858937 1103 28-29 -0.04501156122903893 1103 30-31 0.004889200289916573 1103 32-33 -0.002479531456977213 1103 34-35 0.013940681672828248 1103 36-37 0.010018845481440053 1103 38-39 -0.07151491603215732 1103 40-41 -0.09273912534165163 1103 42-43 0.023242303249439544 1103 44-45 0.23296011974422726 1103 46-47 0.26263723749960377 1103 48-49 0.3081332650295394 1103 50-51 0.243504760930783 1103 52-53 0.24135098175003122 1103 54-55 5.927577935693762E-5 1103 56-57 0.19143539753076766 1103 58-59 0.1161445869795088 1103 60-61 0.1082073484707351 1103 62-63 0.15484280604286482 1103 64-65 0.062307730971554065 1103 66-67 -0.07149567232975329 1103 68-69 -0.0035765428448684133 1103 70-71 0.036137892406031824 1103 72-73 0.15364927931742045 1103 74-75 0.04224233727353521 1103 76-77 0.16281170543335577 1103 78-79 -0.02673971194484892 1103 80-81 0.030464891404850647 1103 82-83 0.0926335470158044 1103 84-85 -0.0824733188413802 1103 86-87 -0.147525606216373 1103 88-89 -0.06070400398223441 1103 90-91 0.07316366055347956 1103 92-93 0.13165503560060543 1103 94-95 -0.06512186088516003 1103 96-97 0.07115209741730055 1103 98-99 0.06210357911921349 1103 100-101 -0.020850474201974833 1104 1 0.034721930143820856 1104 2 -0.015482508722971744 1104 3 -0.003716473979377355 1104 4 0.03215501107673191 1104 5 -0.04601374990878071 1104 6 -0.08897846050916058 1104 7 -0.006870248767853582 1104 8 -0.05146616612889687 1104 9 -0.04727743415141106 1104 10-11 0.03257665051803116 1104 12-13 0.03838303924980124 1104 14-15 0.010658471937851743 1104 16-17 0.018426861941399864 1104 18-19 -0.02541316264517235 1104 20-21 -0.09345406306973558 1104 22-23 -0.03750257339948604 1104 24-25 0.030113545006074105 1104 26-27 0.11022101704278242 1104 28-29 0.025608082179481073 1104 30-31 -0.04892263782941342 1104 32-33 -0.009296276568768747 1104 34-35 0.08072778037180939 1104 36-37 0.0633766511879017 1104 38-39 0.05510979501441682 1104 40-41 0.10370878167584863 1104 42-43 0.14268542636654757 1104 44-45 0.24494594955169902 1104 46-47 0.18691840065709897 1104 48-49 0.13845918780282318 1104 50-51 0.21121473723034256 1104 52-53 0.1702259306355245 1104 54-55 0.19437549931456743 1104 56-57 0.21080779486363355 1104 58-59 0.23833980892077022 1104 60-61 0.17326098740621632 1104 62-63 0.00934797590992531 1104 64-65 0.020161735427905114 1104 66-67 0.15330878861042407 1104 68-69 0.1830122628699371 1104 70-71 0.3115282403184736 1104 72-73 0.39523781381241463 1104 74-75 0.2901630722375259 1104 76-77 0.18948148069643622 1104 78-79 0.12471360903755269 1104 80-81 0.23742387044999091 1104 82-83 0.32794751525387866 1104 84-85 0.33372705836280403 1104 86-87 0.18369742827444568 1104 88-89 0.19684399149465293 1104 90-91 0.2779359297871373 1104 92-93 0.28827952758501496 1104 94-95 0.25139323562737204 1104 96-97 0.44494334369324307 1104 98-99 0.28015362334890526 1104 100-101 0.4376591520153852 1105 1 0.04468423417653966 1105 2 0.0871461734548653 1105 3 0.13413190794788576 1105 4 0.10624248328301178 1105 5 0.13060988941419538 1105 6 0.10519967645704043 1105 7 0.07972667095102537 1105 8 0.10532726185204666 1105 9 0.14119989338640693 1105 10-11 0.16039851668793403 1105 12-13 0.1650076978065087 1105 14-15 0.09841261394691259 1105 16-17 0.20705840738197878 1105 18-19 0.18383936270239332 1105 20-21 0.029796461443616806 1105 22-23 0.19929529216828 1105 24-25 0.22471380153935883 1105 26-27 0.39306872470772447 1105 28-29 0.28378962809047437 1105 30-31 0.3970212039604277 1105 32-33 0.34241705532196676 1105 34-35 0.44753243560423783 1105 36-37 0.41569870081988114 1105 38-39 0.5124935945295803 1105 40-41 0.4675646369383841 1105 42-43 0.5215680796357844 1105 44-45 0.5469755575073734 1105 46-47 0.4192644101218619 1105 48-49 0.4294906366514084 1105 50-51 0.42959975355376656 1105 52-53 0.44126754046031635 1105 54-55 0.5301680868196996 1105 56-57 0.48824258742605053 1105 58-59 0.4043406807576204 1105 60-61 0.45776157856177946 1105 62-63 0.41673240264583455 1105 64-65 0.34139253071245435 1105 66-67 0.28565801258154266 1105 68-69 0.2990063136810974 1105 70-71 0.4739559139253444 1105 72-73 0.5506521401616453 1105 74-75 0.44296721942114914 1105 76-77 0.4104515089527325 1105 78-79 0.30235993179907794 1105 80-81 0.41854695625065474 1105 82-83 0.4031390527914738 1105 84-85 0.36391284114998257 1105 86-87 0.5047810618897124 1105 88-89 0.48736245216947793 1105 90-91 0.5724774627773819 1105 92-93 0.6259083822485607 1105 94-95 0.5311441976272029 1105 96-97 0.7411854289513577 1105 98-99 0.8172375799828622 1105 100-101 0.8322613686135263 1106 1 -0.0853644620932279 1106 2 -0.0640574441981947 1106 3 -0.08036231574517672 1106 4 -0.10939736832369817 1106 5 -0.05152398188258189 1106 6 -0.12083280535269125 1106 7 -0.05715423663561836 1106 8 -0.04429287702109974 1106 9 -0.06037754086040792 1106 10-11 -0.3388054938597733 1106 12-13 -0.359733520620253 1106 14-15 -0.23074961376576653 1106 16-17 -0.23977803510807405 1106 18-19 -0.09703205118945135 1106 20-21 -0.1617586646982332 1106 22-23 -0.1869188278610281 1106 24-25 -0.26597468966962623 1106 26-27 -0.21897821864744316 1106 28-29 -0.11412321352008803 1106 30-31 -0.19902652140621058 1106 32-33 -0.25611176198957963 1106 34-35 -0.2576271400695376 1106 36-37 -0.35863904502261335 1106 38-39 -0.1380850750686875 1106 40-41 -0.2460335791687811 1106 42-43 -0.08527725965141286 1106 44-45 -0.01880039053986593 1106 46-47 -0.3575599999694816 1106 48-49 -0.28796219710126536 1106 50-51 0.08383678236351955 1106 52-53 -0.17013405454085273 1106 54-55 -0.12431053442229967 1106 56-57 0.06289427120629654 1106 58-59 0.0529641327904713 1106 60-61 -2.9587850314527486E-4 1106 62-63 -0.26063762100339716 1106 64-65 -0.1563838366742658 1106 66-67 -0.13939846602458061 1106 68-69 -0.20921821805647767 1106 70-71 0.006664110312222249 1106 72-73 -0.06586662433579704 1106 74-75 -0.02770621712258503 1106 76-77 -0.1636881839849842 1106 78-79 -0.10865056302150933 1106 80-81 -0.1322107826406267 1106 82-83 0.007233090615141435 1106 84-85 -0.06849227856955054 1106 86-87 0.11517369209273554 1106 88-89 0.11397794808564043 1106 90-91 0.12403185224176028 1106 92-93 -0.09110480616709182 1106 94-95 -0.03726282234242717 1106 96-97 0.10324653918097582 1106 98-99 -0.0074491432001870805 1106 100-101 -0.23265833354210486 1107 1 -0.07350058520493263 1107 2 0.031216973131396486 1107 3 -0.07526685814605116 1107 4 -0.07564326682829403 1107 5 -0.011848412496021865 1107 6 0.09613164807174712 1107 7 -0.08161154470086274 1107 8 -0.07887425796647562 1107 9 0.0198609508384493 1107 10-11 -0.05612383082290506 1107 12-13 0.06901531810023442 1107 14-15 0.01941960729278236 1107 16-17 -0.020825022719392905 1107 18-19 -0.06522642442003956 1107 20-21 0.0027780376571584497 1107 22-23 -0.10356201510582252 1107 24-25 -0.04371922256027716 1107 26-27 -0.011023094379957854 1107 28-29 -0.0743993103686833 1107 30-31 -0.11374957845232103 1107 32-33 -0.09530067276438814 1107 34-35 -0.09633937627505418 1107 36-37 -0.03000519201140861 1107 38-39 0.08843666645292103 1107 40-41 0.01571998674197772 1107 42-43 0.005192019957611649 1107 44-45 0.022178709813296393 1107 46-47 -0.16263648365742256 1107 48-49 -0.11828339559248491 1107 50-51 0.0070998974186125 1107 52-53 -0.1936467918099325 1107 54-55 -0.2613381326847346 1107 56-57 -0.2910563213212356 1107 58-59 -0.19314092725762322 1107 60-61 -0.345244439531875 1107 62-63 -0.34908016114686546 1107 64-65 -0.33570570020425095 1107 66-67 -0.3363163582747646 1107 68-69 -0.1669398172631702 1107 70-71 -0.07210839820383086 1107 72-73 -0.11477426009762226 1107 74-75 -0.03605304583533808 1107 76-77 0.04904817552854013 1107 78-79 -0.03804519160667397 1107 80-81 -0.05376254397801361 1107 82-83 -0.03472738611442594 1107 84-85 0.06598388918266807 1107 86-87 0.10647594120982617 1107 88-89 -0.10169291610937847 1107 90-91 -0.050118404212753376 1107 92-93 -0.0753421818530704 1107 94-95 -0.12783342306026313 1107 96-97 0.13003211748548082 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2316 68-69 -0.6152059236494694 2316 70-71 -0.8875519869832971 2316 72-73 -0.7466364747550358 2316 74-75 -0.7149048537824001 2316 76-77 -0.8309348132266265 2316 78-79 -0.6171789575882016 2316 80-81 -0.7260065644489124 2316 82-83 -0.627961350050203 2316 84-85 -0.6648952515676818 2316 86-87 -0.6519072633910561 2316 88-89 -0.7607359044244362 2316 90-91 -0.9530335651737651 2316 92-93 -0.997855004868196 2316 94-95 -1.05492636588124 2316 96-97 -0.7681687855073669 2316 98-99 -0.9796862133076658 2316 100-101 -1.4198282390964287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 20.0 5 37.0 6 111.0 7 308.0 8 779.0 9 1369.0 10 2473.0 11 4071.0 12 6283.0 13 8884.0 14 10375.0 15 13491.0 16 16967.0 17 21641.0 18 19517.0 19 14660.0 20 13414.0 21 13876.0 22 14732.0 23 16416.0 24 19472.0 25 23361.0 26 29540.0 27 36908.0 28 46266.0 29 58435.0 30 71936.0 31 88913.0 32 110674.0 33 139675.0 34 181911.0 35 242077.0 36 341692.0 37 450637.0 38 414623.0 39 90891.0 40 59.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.59077015553742 20.41021266957396 14.382350646815341 19.616666528073278 2 14.46869265492176 24.828014243349752 38.21483930315884 22.488453798569648 3 24.67612018129905 26.18866140497658 28.833034850612798 20.302183563111576 4 12.473693855652542 17.249864535972872 40.9323803948623 29.344061213512283 5 11.77707825961676 40.345204692181 34.19393065745868 13.683786390743563 6 27.369204341252185 40.5640698843441 17.117270805791502 14.949454968612208 7 26.868226056510565 33.8719042660185 22.052989490208 17.20688018726294 8 24.16242859195385 32.35791317715687 19.60474189402656 23.87491633686272 9 22.925311696022167 22.31248763111023 23.09297447061152 31.669226202256084 10-11 24.81593545832234 26.297119938855918 28.11101877841267 20.775925824409068 12-13 29.05201699900376 24.163398312527878 26.509765877789082 20.274818810679278 14-15 22.232104406530635 27.476160054970805 29.074916720497523 21.216818818001038 16-17 19.735713542167606 29.73531377980852 29.229752927071868 21.29921975095201 18-19 19.34701391370912 32.61528493358997 28.90604436728899 19.13165678541192 20-21 22.230783587960843 27.59762513476234 27.97087253160025 22.20071874567657 22-23 20.393936119558234 28.737985854745357 28.204441636872495 22.66363638882391 24-25 23.19967417388713 28.714336546868573 28.371391897331527 19.71459738191277 26-27 22.851317266570927 26.067711095946155 31.58288907220543 19.49808256527749 28-29 19.879548356808254 31.712421058631758 29.379786259590336 19.02824432496966 30-31 21.67406965232423 28.24323805444708 28.131272926923213 21.951419366305483 32-33 21.58160911532639 29.025188033986737 28.911109892835025 20.482092957851847 34-35 23.57341708166769 27.09976135049731 29.811871041392962 19.51495052644204 36-37 22.956816964030335 26.593173762427487 30.81884090817768 19.6311683653645 38-39 20.822982079465962 29.004431049169842 31.193988435096255 18.978598436267944 40-41 21.08463673744056 29.525351436672125 28.783596158318446 20.606415667568864 42-43 19.817150280170086 30.617343907641438 27.309864512224614 22.255641299963862 44-45 22.214398490244015 26.937056427050692 27.88395888226444 22.96458620044085 46-47 23.770363653419217 29.792313394939 27.332090770667232 19.105232180974543 48-49 23.160667658552285 29.007726496051255 28.25680654786996 19.5747992975265 50-51 19.620831339469092 29.468086495908768 30.849819572602584 20.061262592019556 52-53 21.729874636899016 31.5625048238651 26.755223924312716 19.952396614923167 54-55 20.27171174679923 29.33293911223467 27.64647629270679 22.748872848259314 56-57 22.560730762052888 27.70104872096652 28.260804600590117 21.477415916390473 58-59 25.525743277889585 27.305897558850813 26.631346317991195 20.537012845268414 60-61 21.779112630688314 29.056035783734036 30.28437974696071 18.880471838616945 62-63 20.71869488274343 28.65939987296867 29.69473093129719 20.927174312990704 64-65 20.20401569022497 32.32159021522978 27.605949804268164 19.868444290277083 66-67 21.925610352838337 28.0363044535131 27.037953756181913 23.000131437466646 68-69 20.784717686872884 29.68893110886818 27.065071543074275 22.461279661184665 70-71 23.30559065582027 29.235709189716573 26.98062630306641 20.47807385139675 72-73 22.80123775925677 26.84701094517675 29.861892000042744 20.48985929552373 74-75 20.362691398636176 32.12172330858434 27.830728709533204 19.684856583246273 76-77 21.784555003144664 28.487600044488403 26.95344272573017 22.77440222663676 78-79 21.6553644744253 29.519165042879457 27.58350382604242 21.241966656652817 80-81 23.316559802778585 28.412818441951625 27.87074453379289 20.3998772214769 82-83 22.274402424538916 27.753462199302515 29.22581467412837 20.7463207020302 84-85 20.397783494953494 29.477735998416783 29.184820898476154 20.939659608153573 86-87 21.312255020120855 29.66610105826954 26.918566244063385 22.103077677546217 88-89 20.50416762154458 30.075725281723614 26.328408340230226 23.09169875650158 90-91 22.207669816873217 26.045804058656618 27.470481903628773 24.276044220841392 92-93 22.940145283932207 28.952234730957954 27.35419459560871 20.753425389501132 94-95 21.93999361962685 28.590266052060997 28.404633607467726 21.065106720844426 96-97 18.78901163273949 29.11723379075318 30.610115219138013 21.483639357369313 98-99 20.897578514668496 31.15904240929053 26.564559121926834 21.378819954114142 100-101 19.34525499962497 29.52610258327398 27.0481734455402 24.08046897156085 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 295.0 1 214.5 2 269.5 3 422.5 4 367.0 5 314.0 6 443.5 7 778.5 8 1160.5 9 1403.0 10 1516.5 11 1549.5 12 1712.0 13 2001.5 14 2282.5 15 2553.0 16 2816.0 17 3077.5 18 3400.0 19 3804.5 20 4201.0 21 4796.0 22 5597.5 23 6836.5 24 8552.0 25 10571.5 26 13616.0 27 17772.5 28 23196.0 29 29890.0 30 36817.5 31 43107.0 32 48576.5 33 53691.0 34 58585.5 35 63037.0 36 67098.0 37 71635.0 38 76736.0 39 79135.0 40 79670.5 41 80654.0 42 81672.0 43 83672.0 44 84652.0 45 86117.5 46 93610.0 47 145302.5 48 253246.5 49 243701.5 50 135911.5 51 84770.5 52 62106.5 53 50420.0 54 43006.5 55 36887.5 56 31003.5 57 24420.5 58 18910.0 59 14993.5 60 11779.0 61 8852.5 62 6347.0 63 4363.0 64 2798.0 65 1705.5 66 1026.5 67 578.0 68 286.5 69 145.0 70 84.5 71 48.5 72 26.5 73 10.5 74 9.5 75 9.0 76 5.5 77 3.0 78 2.0 79 3.5 80 4.0 81 1.5 82 0.5 83 0.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044646726836838777 2 0.00510587567548954 3 0.03760141001329507 4 0.0 5 0.0 6 0.0016227976953106292 7 0.0 8 0.0 9 3.9580431592942175E-5 10-11 3.760141001329507E-4 12-13 0.0 14-15 0.00273104977991301 16-17 0.0 18-19 0.0 20-21 0.01165643710412147 22-23 0.0 24-25 0.0 26-27 0.008272310202924914 28-29 0.010251331782572023 30-31 0.010607555666908504 32-33 0.006431820133853104 34-35 0.00799524718177432 36-37 0.01969126471748873 38-39 0.009380562287527296 40-41 0.010429443724740263 42-43 0.0 44-45 0.0 46-47 7.916086318588435E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0016821683427000423 58-59 2.572728053541241E-4 60-61 0.008450422145093154 62-63 0.013952102136512117 64-65 0.04381553777338699 66-67 0.023154552481871173 68-69 0.002018602011240051 70-71 3.3643366854000853E-4 72-73 0.0 74-75 1.9790215796471088E-5 76-77 0.0 78-79 0.0 80-81 1.9790215796471088E-5 82-83 0.0 84-85 3.9580431592942175E-5 86-87 7.124477686729592E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.1874129477882653E-4 96-97 4.3538474752236397E-4 98-99 5.739162580976616E-4 100-101 0.0014446857531423894 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2526501.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.48439222732569 #Duplication Level Percentage of deduplicated Percentage of total 1 80.72572043437196 42.368403741110185 2 12.34378782345487 12.957124033941843 3 3.06591025058635 4.827373083766571 4 1.254276514407177 2.6331976217467683 5 0.6478799426079276 1.7001792512025868 6 0.37741556922050856 1.1885056060601136 7 0.262261919791697 0.9635260225247197 8 0.19266429219026848 0.8089494623611307 9 0.1406416686308485 0.6643343249934318 >10 0.8775125315024449 8.597904919970965 >50 0.0696884505174727 2.513835860387954 >100 0.03585628512549496 3.4326848605506757 >500 0.002027959706004791 0.7558943558157891 >1k 0.002703946274673017 2.995943423605922 >5k 8.262058061500885E-4 2.878351096961934 >10k+ 8.262058061500885E-4 10.713792334999376 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 91488 3.621134525575094 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 25410 1.0057387667766606 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 21786 0.8622992826838382 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 20310 0.8038785656526557 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 19146 0.7578069432784709 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 19089 0.7555508586776732 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 17677 0.6996632892684388 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15962 0.631782849086543 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 15380 0.6087470378994507 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 13292 0.5261030967333874 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 12240 0.4844644826976123 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9207 0.36441703367621864 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 9080 0.359390318863915 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8026 0.3176725439649539 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 7030 0.27825043409838346 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6687 0.26467434606200435 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 6190 0.24500287156031206 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 5880 0.2327329377665 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5520 0.2184839823930408 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5216 0.2064515311887864 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5155 0.20403712486161693 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5025 0.19889166875453443 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4739 0.18757166531895297 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 4526 0.17914103338965628 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4218 0.1669502604590301 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3877 0.1534533332858368 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3873 0.15329501155946507 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3871 0.15321585069627916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3431 0.1358004607953846 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2877 0.11387290169289463 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2742 0.10852954342784746 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.9580431592942175E-5 0.0 5 0.0 0.0 0.0 3.9580431592942175E-5 0.0 6 0.0 0.0 0.0 3.9580431592942175E-5 0.0 7 0.0 0.0 0.0 3.9580431592942175E-5 0.0 8 0.0 0.0 0.0 3.9580431592942175E-5 0.0 9 0.0 0.0 0.0 3.9580431592942175E-5 0.0 10-11 0.0 0.0 0.0 1.583217263717687E-4 0.0 12-13 0.0 0.0 0.0 2.5727280535412417E-4 0.0 14-15 0.0 0.0 0.0 2.968532369470663E-4 0.0 16-17 0.0 0.0 0.0 3.166434527435374E-4 0.0 18-19 0.0 0.0 0.0 4.7496517911530613E-4 0.0 20-21 0.0 0.0 0.0 8.707694950447278E-4 0.0 22-23 0.0 0.0 0.0 0.0013457346741600341 0.0 24-25 0.0 0.0 0.0 0.0019196509322576955 0.0 26-27 0.0 0.0 0.0 0.00290916172208125 0.0 28-29 0.0 0.0 0.0 0.006313078839074277 0.0 30-31 0.0 0.0 0.0 0.013259444583635628 0.0 32-33 0.0 0.0 0.0 0.026538679383067726 0.0 34-35 0.0 0.0 0.0 0.04229169115705871 0.0 36-37 0.0 1.9790215796471088E-5 0.0 0.06144862004804273 0.0 38-39 0.0 3.9580431592942175E-5 0.0 0.08889764935774813 0.0 40-41 0.0 3.9580431592942175E-5 0.0 0.11618835694108176 0.0 42-43 0.0 3.9580431592942175E-5 0.0 0.14019388870220117 0.0 44-45 0.0 3.9580431592942175E-5 0.0 0.1562635439289357 0.0 46-47 0.0 3.9580431592942175E-5 0.0 0.17961599856877158 0.0 48-49 0.0 3.9580431592942175E-5 0.0 0.19703138846966614 0.0 50-51 0.0 7.916086318588435E-5 0.0 0.21312083391219713 0.0 52-53 0.0 7.916086318588435E-5 0.0 0.25297832852628993 0.0 54-55 0.0 7.916086318588435E-5 0.0 0.32127436323991165 0.0 56-57 0.0 7.916086318588435E-5 0.0 0.43077362724178614 0.0 58-59 0.0 7.916086318588435E-5 0.0 0.5843457018224019 0.0 60-61 0.0 7.916086318588435E-5 0.0 0.7534135153716544 0.0 62-63 0.0 7.916086318588435E-5 0.0 0.8245395509441713 0.0 64-65 0.0 7.916086318588435E-5 0.0 0.924420770068961 0.0 66-67 0.0 1.583217263717687E-4 0.0 1.2663363283845919 0.0 68-69 0.0 1.583217263717687E-4 0.0 1.638748609242585 0.0 70-71 0.0 1.583217263717687E-4 0.0 1.86892860917134 0.0 72-73 0.0 1.583217263717687E-4 0.0 2.1465061759326436 0.0 74-75 0.0 1.583217263717687E-4 0.0 2.3710855447909975 0.0 76-77 0.0 1.583217263717687E-4 0.0 2.514604189747006 0.0 78-79 0.0 1.583217263717687E-4 0.0 2.648841223494469 0.0 80-81 0.0 1.583217263717687E-4 0.0 2.788540356801759 0.0 82-83 0.0 1.583217263717687E-4 0.0 3.017889167667062 0.0 84-85 0.0 1.583217263717687E-4 0.0 3.6135944533566384 0.0 86-87 0.0 1.583217263717687E-4 0.0 4.647039522248359 0.0 88-89 0.0 1.583217263717687E-4 0.0 5.835164917805297 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2980 0.0 46.241753 2 TATAACG 495 0.0 44.15757 2 GTACCCG 345 0.0 42.713696 1 ACCTGGG 3170 0.0 42.417522 3 ATAACGC 585 0.0 38.173336 3 TAACGCA 550 0.0 37.99359 4 GTACCTG 3920 0.0 36.379753 1 GTACCGG 165 1.8189894E-12 34.57179 1 GTACACG 340 0.0 33.55497 1 TCACGCA 385 0.0 32.072517 4 TACCCGG 450 0.0 30.622316 2 TACACGG 365 0.0 29.94246 2 CCTGGGG 2325 0.0 29.822931 4 GTATAAC 820 0.0 28.985495 1 GAGTACG 1435 0.0 28.4886 1 AGTACGC 1325 0.0 27.613876 2 GTACGCA 1325 0.0 27.61169 3 TACGCAG 1365 0.0 27.138283 4 TATCACG 465 0.0 25.54698 2 ACGCAGT 1565 0.0 23.367132 5 >>END_MODULE