##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003852_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3575663 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.846562441706617 31.0 31.0 34.0 28.0 34.0 2 30.805740921334028 31.0 31.0 34.0 27.0 34.0 3 30.87062175602119 31.0 31.0 34.0 27.0 34.0 4 34.36334911875085 37.0 35.0 37.0 30.0 37.0 5 34.31919954425235 37.0 35.0 37.0 30.0 37.0 6 34.41846421209158 37.0 35.0 37.0 30.0 37.0 7 34.44025401722701 37.0 35.0 37.0 30.0 37.0 8 34.41624728057426 37.0 35.0 37.0 30.0 37.0 9 35.88074575260588 39.0 35.0 39.0 30.0 39.0 10-11 35.72840715134508 39.0 35.0 39.0 30.0 39.0 12-13 35.58572256389934 39.0 35.0 39.0 30.0 39.0 14-15 36.59742752602804 39.0 36.0 41.0 30.0 41.0 16-17 36.65879908145706 39.0 36.0 41.0 30.0 41.0 18-19 36.62113291996478 39.0 36.0 41.0 30.0 41.0 20-21 36.50606083403274 39.0 36.0 41.0 29.5 41.0 22-23 36.43116395476867 39.0 36.0 41.0 29.5 41.0 24-25 36.24216613813998 39.0 36.0 41.0 29.0 41.0 26-27 36.06878150989061 39.0 35.5 41.0 27.0 41.0 28-29 35.89967720671663 39.0 35.0 40.0 27.0 41.0 30-31 35.701442361878065 38.5 35.0 40.0 27.0 41.0 32-33 35.49128916791096 38.0 35.0 40.0 27.0 41.0 34-35 35.504117418224254 38.0 35.0 40.0 26.5 41.0 36-37 35.39880268358623 38.0 35.0 40.0 26.0 41.0 38-39 35.42971107735824 38.5 35.0 40.0 26.5 41.0 40-41 35.54167800489029 39.0 35.0 40.0 26.5 41.0 42-43 35.42492133626687 39.0 35.0 40.5 26.0 41.0 44-45 35.23966855936927 39.0 35.0 40.0 25.0 41.0 46-47 35.00130912784566 38.0 34.0 40.0 24.0 41.0 48-49 34.77155173739807 38.0 34.0 40.0 24.0 41.0 50-51 34.028140655313436 37.5 33.5 39.5 23.0 40.5 52-53 34.186031653430426 38.0 33.5 40.0 23.0 40.5 54-55 34.542609300708705 38.0 34.0 40.0 23.5 41.0 56-57 34.37612395239708 38.0 34.0 40.0 23.0 41.0 58-59 34.118131238877936 38.0 33.5 40.0 22.0 41.0 60-61 33.85341417801398 38.0 33.0 40.0 22.0 41.0 62-63 33.61238545131351 37.5 33.0 40.0 21.0 41.0 64-65 33.32504167758539 37.0 32.5 40.0 20.5 41.0 66-67 32.96989551308387 36.5 32.0 39.0 19.5 41.0 68-69 32.61198272879743 36.0 32.0 39.0 18.0 41.0 70-71 32.201910526803005 35.5 31.0 39.0 17.5 40.5 72-73 31.837585644955915 35.0 31.0 38.0 14.5 40.0 74-75 31.469611230141098 35.0 31.0 37.5 13.0 39.5 76-77 31.043966112018946 35.0 30.5 37.0 9.5 39.0 78-79 30.499810804317967 34.5 30.0 36.0 7.0 39.0 80-81 30.096325772311317 34.0 29.5 36.0 7.0 38.5 82-83 29.756804961765134 34.0 29.0 35.5 6.5 37.0 84-85 29.511289654534 34.0 29.0 35.0 2.0 37.0 86-87 29.290770550804147 34.0 29.0 35.0 2.0 36.5 88-89 28.972962356911154 34.0 29.0 35.0 2.0 36.0 90-91 28.63880237035761 34.0 29.0 35.0 2.0 36.0 92-93 28.3939275037944 34.0 29.0 35.0 2.0 35.5 94-95 28.17292709072415 34.0 27.5 35.0 2.0 35.0 96-97 27.888741192892063 34.0 27.0 35.0 2.0 35.0 98-99 27.637310479203435 34.0 26.5 35.0 2.0 35.0 100-101 27.367590569916686 34.0 25.5 35.0 2.0 35.0 102-103 27.07876804944985 33.0 25.0 35.0 2.0 35.0 104-105 26.795560012227103 33.0 25.0 35.0 2.0 35.0 106-107 26.45271044838398 33.0 25.0 35.0 2.0 35.0 108-109 26.14630839091939 33.0 24.0 35.0 2.0 35.0 110-111 25.79576095398252 32.0 23.0 35.0 2.0 35.0 112-113 25.336139339753217 32.0 19.5 35.0 2.0 35.0 114-115 24.843176076716404 32.0 18.0 34.0 2.0 35.0 116-117 24.207265477758952 32.0 6.0 34.0 2.0 35.0 118-119 23.47028634969235 31.0 2.0 34.0 2.0 35.0 120-121 21.81019645867074 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2839492705560147 1101 2 0.2620342222550036 1101 3 0.30240799265216367 1101 4 0.5218865068622591 1101 5 0.5386307707252698 1101 6 0.5497889257620727 1101 7 0.2745522773570954 1101 8 0.30258399428092986 1101 9 0.41712499539361403 1101 10-11 0.5600474085978746 1101 12-13 0.6278141875348098 1101 14-15 0.6971551171442698 1101 16-17 0.5133253626305034 1101 18-19 0.4555411108027698 1101 20-21 0.568981147714652 1101 22-23 0.4893281363341089 1101 24-25 0.4979407644917515 1101 26-27 0.4634263737248574 1101 28-29 0.6551259128220366 1101 30-31 0.5128198557459953 1101 32-33 0.5456060349546945 1101 34-35 0.5667324407652927 1101 36-37 0.4851323979327802 1101 38-39 0.5712299819132909 1101 40-41 0.5400003753263078 1101 42-43 0.5274634935687814 1101 44-45 0.5723709048432042 1101 46-47 0.5543534316468453 1101 48-49 0.7430094504853386 1101 50-51 0.31642076958064536 1101 52-53 0.345074422974605 1101 54-55 0.5625760610387331 1101 56-57 0.5732584642998759 1101 58-59 0.6103936881253773 1101 60-61 0.5546258024141295 1101 62-63 0.5445698953425406 1101 64-65 0.47585492362326676 1101 66-67 0.4616612097961337 1101 68-69 0.48117255895856204 1101 70-71 0.340935542626557 1101 72-73 0.25248315415542777 1101 74-75 0.19216998112098338 1101 76-77 0.10212268296762161 1101 78-79 0.4979534694409722 1101 80-81 0.6481511327350056 1101 82-83 0.6231072288896868 1101 84-85 0.5523122573368404 1101 86-87 0.4759951951569583 1101 88-89 0.5734043811364593 1101 90-91 0.6668993049726808 1101 92-93 0.5786730134978733 1101 94-95 0.6425782091516794 1101 96-97 0.5233234063441969 1101 98-99 0.5339295560641517 1101 100-101 0.5646215034456539 1101 102-103 0.447363947736779 1101 104-105 0.34962569964398327 1101 106-107 0.24358554513802844 1101 108-109 0.35611737644959973 1101 110-111 0.25642147923894143 1101 112-113 0.1775337148752456 1101 114-115 0.168369343198691 1101 116-117 0.02712050685661893 1101 118-119 0.19263897491100934 1101 120-121 0.3256497270648673 1102 1 0.4663495215244211 1102 2 0.5501525011937112 1102 3 0.6403133403173307 1102 4 0.8681588975341228 1102 5 0.885226414019705 1102 6 0.7283975253218173 1102 7 0.5761429536578717 1102 8 0.7768561143426354 1102 9 0.7191437267841394 1102 10-11 0.7408230068953188 1102 12-13 0.7475525341035478 1102 14-15 0.8185769505055518 1102 16-17 0.7178201715847123 1102 18-19 0.6657021147321416 1102 20-21 0.841965548336475 1102 22-23 0.6670802504625541 1102 24-25 0.7423622846250026 1102 26-27 0.8492093213288214 1102 28-29 0.8435291472566817 1102 30-31 0.7856370816906235 1102 32-33 0.53000713924569 1102 34-35 0.6290149923981971 1102 36-37 0.6633317317965037 1102 38-39 0.5673751005762213 1102 40-41 0.6093784024356736 1102 42-43 0.5920676476812687 1102 44-45 0.6281470459155827 1102 46-47 0.7319249424443726 1102 48-49 0.6075031572714025 1102 50-51 0.5436829629992488 1102 52-53 0.5082425903000214 1102 54-55 0.47085382628131356 1102 56-57 0.511977084471134 1102 58-59 0.4666984624915571 1102 60-61 0.6567610602102434 1102 62-63 0.5830207679932826 1102 64-65 0.5039784110569698 1102 66-67 0.4402005861201417 1102 68-69 0.3486580679401783 1102 70-71 0.24431643269273096 1102 72-73 0.2991457423710955 1102 74-75 0.31660347821956236 1102 76-77 0.22417343834681347 1102 78-79 0.4866171393467589 1102 80-81 0.5952020315139954 1102 82-83 0.6407172062132176 1102 84-85 0.44799847521199965 1102 86-87 0.5029565277640167 1102 88-89 0.3593981452020394 1102 90-91 0.5776029926954216 1102 92-93 0.5029501217281585 1102 94-95 0.292750602509269 1102 96-97 0.5114883666069048 1102 98-99 0.6886780379159809 1102 100-101 0.6768215238057458 1102 102-103 0.5389637699960801 1102 104-105 0.6176149091176342 1102 106-107 0.7135004021126825 1102 108-109 0.657015471944856 1102 110-111 0.4935978252309674 1102 112-113 0.3640724035235188 1102 114-115 0.4759528979707923 1102 116-117 0.5891147310881202 1102 118-119 0.4267371035785281 1102 120-121 0.5220795439082195 1103 1 0.5335244933234371 1103 2 0.6490275627275928 1103 3 0.7148984467579247 1103 4 0.7676552526392157 1103 5 0.7690099126010068 1103 6 0.8123313775663448 1103 7 0.7066408509265969 1103 8 0.6943890762973055 1103 9 0.7877393270319502 1103 10-11 0.8314422465100293 1103 12-13 0.8651301760103038 1103 14-15 1.00748041925182 1103 16-17 0.9770230840962242 1103 18-19 0.8681712192326998 1103 20-21 0.946452327338072 1103 22-23 0.9367789937875628 1103 24-25 0.9648851407684731 1103 26-27 0.9773794210668356 1103 28-29 1.0444914714093514 1103 30-31 0.9438602373094014 1103 32-33 0.8984777106199644 1103 34-35 0.9072149789590895 1103 36-37 0.864844743649634 1103 38-39 0.8588790532372528 1103 40-41 1.0073357781065866 1103 42-43 0.9518299731909323 1103 44-45 0.9785041730642945 1103 46-47 0.8950478050514619 1103 48-49 0.9702502369005828 1103 50-51 0.7908470478170031 1103 52-53 0.9456292350832172 1103 54-55 0.7418368727184088 1103 56-57 0.7666828976084687 1103 58-59 0.729479401751405 1103 60-61 0.8959411107753041 1103 62-63 0.8873862915447717 1103 64-65 0.9236527312857561 1103 66-67 0.8462332962255772 1103 68-69 0.762707606561797 1103 70-71 0.7898424589285007 1103 72-73 0.7016243220926128 1103 74-75 0.5234374634802386 1103 76-77 0.6792429166543634 1103 78-79 0.8208225263074631 1103 80-81 0.6866279445411081 1103 82-83 0.7095791184973166 1103 84-85 0.7995299167270034 1103 86-87 0.805065139388816 1103 88-89 0.9499371251718856 1103 90-91 0.9147143620737559 1103 92-93 0.8399219815717878 1103 94-95 0.8975129187936943 1103 96-97 0.8273715211817461 1103 98-99 0.8742978545549818 1103 100-101 0.809044423141227 1103 102-103 0.9078082614436553 1103 104-105 1.033934185734509 1103 106-107 0.9606976383002426 1103 108-109 1.1203874340637334 1103 110-111 1.2643063164276107 1103 112-113 1.1790882658885522 1103 114-115 1.2423380517755902 1103 116-117 0.9745862712323188 1103 118-119 0.9714438981630558 1103 120-121 1.091889067564697 1104 1 0.47064511706464884 1104 2 0.654371363040525 1104 3 0.6876902952640762 1104 4 0.8996427590555598 1104 5 1.0165284736279574 1104 6 0.8545803901186773 1104 7 0.7028755624949881 1104 8 0.72531574645474 1104 9 0.7521886986936437 1104 10-11 0.842615131794993 1104 12-13 0.9363624975533611 1104 14-15 0.9928092068557461 1104 16-17 0.8450659200500397 1104 18-19 0.8267340744510179 1104 20-21 0.7815282747415395 1104 22-23 0.7197539994050857 1104 24-25 0.8738636349210935 1104 26-27 0.9090727868718673 1104 28-29 0.926822825810298 1104 30-31 0.8117672090197559 1104 32-33 0.9000977419441156 1104 34-35 0.907419677220652 1104 36-37 0.8315713713921724 1104 38-39 0.7250774145173509 1104 40-41 0.7424940750892048 1104 42-43 0.7520668884786446 1104 44-45 0.8495003652578959 1104 46-47 0.8958059455679503 1104 48-49 0.8742608604665705 1104 50-51 0.6594088284902426 1104 52-53 0.7284025380235022 1104 54-55 0.7726477136156404 1104 56-57 0.9741830290276496 1104 58-59 0.796542514139027 1104 60-61 0.6726236499588438 1104 62-63 0.8060551788520556 1104 64-65 0.939835618601272 1104 66-67 0.7555655592226742 1104 68-69 0.7797638194423229 1104 70-71 0.7001254173134619 1104 72-73 0.6730235459979141 1104 74-75 0.5582786859157096 1104 76-77 0.6371562776561568 1104 78-79 0.5342266000959839 1104 80-81 0.6437649016252713 1104 82-83 0.6040663607868737 1104 84-85 0.656551096087906 1104 86-87 0.6543164494576921 1104 88-89 0.873187162793851 1104 90-91 0.8917071471606199 1104 92-93 0.9077328948784178 1104 94-95 0.7756675267625397 1104 96-97 0.6615383248507598 1104 98-99 0.7604284473431093 1104 100-101 0.8531012547226453 1104 102-103 0.7824532685969814 1104 104-105 0.8262956454357173 1104 106-107 0.8388535669030368 1104 108-109 0.7480699637129327 1104 110-111 0.7641301889540948 1104 112-113 0.6638672832441301 1104 114-115 0.8406375712967531 1104 116-117 0.6870691545279612 1104 118-119 0.6853821844878354 1104 120-121 0.7773929454613331 1105 1 0.7211593512931209 1105 2 0.7932648659978732 1105 3 0.7753219664668407 1105 4 1.0537701641942618 1105 5 1.0839889426581308 1105 6 0.9950826978437846 1105 7 0.924359138977394 1105 8 0.9386102150206455 1105 9 1.074709937226082 1105 10-11 1.1466862650378502 1105 12-13 1.238890891099544 1105 14-15 1.3647825013183876 1105 16-17 1.2186964299880003 1105 18-19 1.0810222342282643 1105 20-21 1.161539819288869 1105 22-23 1.2073701851032368 1105 24-25 1.3089815172146757 1105 26-27 1.4248968333437801 1105 28-29 1.3642581671664757 1105 30-31 1.3502503164956678 1105 32-33 1.3873694639914476 1105 34-35 1.3995059699730774 1105 36-37 1.4631873163691864 1105 38-39 1.3010590193367406 1105 40-41 1.2564160396934199 1105 42-43 1.1828940572191229 1105 44-45 1.2206093361321138 1105 46-47 1.3636822211234687 1105 48-49 1.4366736989862119 1105 50-51 1.1416963943110332 1105 52-53 1.205148891379622 1105 54-55 1.35860381176591 1105 56-57 1.3927706532966013 1105 58-59 1.4604828955384264 1105 60-61 1.3572714894496585 1105 62-63 1.4253799116604782 1105 64-65 1.3993150791372244 1105 66-67 1.4144747801152562 1105 68-69 1.2770847235115497 1105 70-71 1.0916682591611568 1105 72-73 1.2043985329699538 1105 74-75 1.102761242072127 1105 76-77 1.1539153021818827 1105 78-79 1.2151678374914319 1105 80-81 1.2104710444851214 1105 82-83 1.2970174138604058 1105 84-85 1.2008469305167715 1105 86-87 1.220830763038272 1105 88-89 1.193845625400055 1105 90-91 1.2905762537861634 1105 92-93 1.265728855915345 1105 94-95 1.3129073155082764 1105 96-97 1.2382066362971251 1105 98-99 1.1858751804642047 1105 100-101 1.2359749158038724 1105 102-103 1.2383387152427545 1105 104-105 1.2881978620243366 1105 106-107 1.3230434736702286 1105 108-109 1.4342447761103365 1105 110-111 1.5356196576265937 1105 112-113 1.3967367768592496 1105 114-115 1.3687722978517556 1105 116-117 1.4742474305856526 1105 118-119 1.4525814947758953 1105 120-121 1.2965090680923161 1106 1 0.4331459437558749 1106 2 0.6618432739097386 1106 3 0.6094479510371968 1106 4 0.927675246183675 1106 5 0.892015455996777 1106 6 0.9064722576815214 1106 7 0.7854124145527734 1106 8 0.8095289446107898 1106 9 0.9665345777297318 1106 10-11 1.0307778625949382 1106 12-13 0.9993907379380218 1106 14-15 1.0825372009476553 1106 16-17 0.9977932968791734 1106 18-19 0.9702283053709806 1106 20-21 1.055809267701811 1106 22-23 0.8283117232730675 1106 24-25 1.115551254638227 1106 26-27 1.101854501809136 1106 28-29 1.1611829759864136 1106 30-31 0.9717478828159685 1106 32-33 0.9573396030159032 1106 34-35 1.1079898951487053 1106 36-37 1.0653055326006395 1106 38-39 1.172031011420259 1106 40-41 1.1737033375826442 1106 42-43 1.0176705322886264 1106 44-45 0.9959210831198106 1106 46-47 1.239214782346039 1106 48-49 1.3496835599379722 1106 50-51 0.9372861579224079 1106 52-53 1.0359449932107054 1106 54-55 1.1547848145381039 1106 56-57 1.2121649287763319 1106 58-59 1.3245071054228887 1106 60-61 1.292314608283803 1106 62-63 1.347383233812927 1106 64-65 1.1508066837959774 1106 66-67 1.276568798015191 1106 68-69 1.214445567814657 1106 70-71 0.9195059990101448 1106 72-73 0.9972938685177155 1106 74-75 0.9739701120866684 1106 76-77 1.0511643920898202 1106 78-79 1.1314490094293888 1106 80-81 0.9723990821844346 1106 82-83 1.174556408036871 1106 84-85 1.2347550910273313 1106 86-87 1.2151681190065489 1106 88-89 1.0632658270567816 1106 90-91 1.0132635954746583 1106 92-93 0.9588056492176271 1106 94-95 0.9493872729254669 1106 96-97 1.2238017227599052 1106 98-99 1.4431098325836693 1106 100-101 1.2434243593339893 1106 102-103 1.0140991159126216 1106 104-105 1.1445794987476283 1106 106-107 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0.9348792637295631 2210 92-93 1.0439632706647437 2210 94-95 0.9977619119206977 2210 96-97 1.2447133846874934 2210 98-99 1.0699721266998274 2210 100-101 1.2080976582048315 2210 102-103 1.2308666117023535 2210 104-105 1.3297797872448847 2210 106-107 1.1968045911213672 2210 108-109 1.3668051380942607 2210 110-111 1.4204772050828147 2210 112-113 1.4543667208177133 2210 114-115 1.5257074933341528 2210 116-117 1.4764904260002147 2210 118-119 1.596676112516679 2210 120-121 1.704208604131633 2211 1 0.37309336138025984 2211 2 0.601677446260787 2211 3 0.6440952539417637 2211 4 0.8051673057436091 2211 5 0.8350132707168925 2211 6 0.7423592129466883 2211 7 0.6159362919057827 2211 8 0.6918575210528246 2211 9 0.7401854401927608 2211 10-11 0.7666923181771352 2211 12-13 0.8227337148141629 2211 14-15 0.9882542440663897 2211 16-17 0.8927343444569331 2211 18-19 0.767381174693611 2211 20-21 0.8075364055749858 2211 22-23 0.8100077924234697 2211 24-25 0.896008334974546 2211 26-27 0.9767045402948398 2211 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-0.9584090109619332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45243.0 3 6758.0 4 6395.0 5 7253.0 6 8484.0 7 8740.0 8 10547.0 9 12452.0 10 14970.0 11 17701.0 12 19880.0 13 23474.0 14 29121.0 15 29335.0 16 22543.0 17 22047.0 18 23520.0 19 25369.0 20 28394.0 21 31680.0 22 35818.0 23 40798.0 24 46129.0 25 52908.0 26 60512.0 27 71454.0 28 83777.0 29 99356.0 30 118145.0 31 142179.0 32 174188.0 33 219434.0 34 283018.0 35 379111.0 36 525206.0 37 615390.0 38 233452.0 39 882.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73065636905131 19.91448739767714 15.011191859281153 22.343664373990393 2 14.012550556808955 20.30969060276636 40.950888316066866 24.726870524357818 3 19.62771511789604 25.24411591648751 30.760918436597557 24.367250529018893 4 11.625035109705847 16.222940145988694 41.185941002157904 30.966083742147553 5 11.69232938721118 37.9540143978227 36.82819209318395 13.525464121782171 6 28.63183761057429 37.63914689478117 18.461569705028044 15.267445789616495 7 25.60995693824431 32.94325831131365 23.471420473933115 17.975364276508923 8 23.658486311418574 34.12503246228665 21.232126004145584 20.98435522214919 9 23.25410838662962 18.52466329021696 23.85060760868523 34.37062071446819 10-11 23.65891851855611 25.928580914028288 28.930695788267847 21.48180477914775 12-13 25.26147744660982 23.830220603929373 28.917946481110608 21.9903554683502 14-15 21.786832839349113 25.67935518142078 28.45141868207717 24.082393297152937 16-17 20.134312513854866 29.341049940688485 28.910470357499225 21.614167187957424 18-19 19.72861264892606 29.58948427968196 30.27583058933984 20.40607248205214 20-21 20.90590083658526 27.47997748344016 29.194671908306656 22.41944977166792 22-23 20.30740599103398 27.20692914949157 29.891277984969395 22.59438687450505 24-25 21.50253208177787 27.274150093031942 29.519634843295183 21.70368298189501 26-27 20.919623339759713 26.61048789212705 31.037019813959745 21.432868954153488 28-29 20.11587398249578 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28.755783726700084 22.027184220650938 54-55 20.097840294612606 27.897136091609866 29.471077618471934 22.533945995305597 56-57 21.354880043351194 27.16487142109222 28.946560339584355 22.533688195972235 58-59 22.02947474741775 26.95745953464489 29.007671277709278 22.00539444022808 60-61 21.300285293723014 27.21283933212967 30.329018255778994 21.157857118368327 62-63 20.58760554162282 27.77897828092338 29.73750356154491 21.89591261590889 64-65 20.15637662285278 28.817383278851572 29.45693402079361 21.569306077502034 66-67 20.55713574272425 27.572818875102623 28.69560348473935 23.174441897433777 68-69 20.249382377142318 27.95684165875168 29.172309900318428 22.621466063787572 70-71 21.31344790337778 27.49396097015535 28.655536954150772 22.537054172316097 72-73 20.74673552919161 26.847440200041422 30.151681712473753 22.254142558293214 74-75 20.3518503939563 28.431897889371687 29.50239297094941 21.713858745722607 76-77 20.590855726953055 27.549119212512206 28.99434726902258 22.865677791512155 78-79 20.720647209735983 27.488259109768975 29.41477166126662 22.37632201922842 80-81 21.48059782761416 27.40723387736883 28.873744705902123 22.238423589114884 82-83 20.609423045243442 26.818148645503065 30.307970914759906 22.26445739449359 84-85 20.882361366063073 27.24716647614941 29.53002936284641 22.340442794941108 86-87 21.04800708791612 27.794492756894634 29.26661794664876 21.89088220854049 88-89 20.416079248138143 27.118498206248482 28.703381964745155 23.76204058086822 90-91 20.10332370523311 26.007341926145873 30.003607891985045 23.88572647663597 92-93 21.004125189047652 26.83441118710639 30.15256427620144 22.008899347644522 94-95 20.337228742314597 26.682373574136843 30.103401221200066 22.876996462348494 96-97 19.073217998781587 27.247188237083414 30.591562919464742 23.088030844670257 98-99 19.870386438603553 27.75542077057392 29.359183597430476 23.015009193392046 100-101 19.10713794332121 26.55441918880112 30.5101232285673 23.828319639310372 102-103 20.37701361919305 26.363485280955818 29.93938403821997 23.320117061631166 104-105 20.7830723656369 26.850768796727326 28.989913951191987 23.376244886443786 106-107 20.54031216562877 26.45137127029503 30.32712571053335 22.681190853542844 108-109 20.434656448771836 26.72441434272615 29.814059837204145 23.026869371297874 110-111 20.267361144907305 26.918804361027682 28.742077761193546 24.071756732871467 112-113 20.99500304726649 25.963655704031417 27.94815978962575 25.09318145907634 114-115 20.50222097130196 25.644730651238024 29.216032753418926 24.63701562404109 116-117 21.23201182244543 25.60610212946831 28.901656773704094 24.26022927438217 118-119 21.087152882779158 25.524349164691916 29.804176843814062 23.584321108714864 120-121 20.687309803656 27.58512542906602 28.52282779374357 23.204736973534406 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8563.0 1 9060.0 2 5544.0 3 1571.5 4 1636.0 5 1667.0 6 1610.0 7 1633.0 8 1764.5 9 1777.5 10 1716.0 11 1758.0 12 1869.0 13 2013.5 14 2239.0 15 2465.0 16 2702.0 17 2960.5 18 3342.5 19 3758.5 20 4120.5 21 4604.0 22 5277.5 23 6271.0 24 7772.5 25 9930.5 26 12995.5 27 17513.5 28 24485.5 29 34298.0 30 45674.5 31 57244.5 32 68808.5 33 79086.5 34 87849.5 35 96537.5 36 105086.0 37 115607.0 38 124746.0 39 128105.0 40 130915.5 41 134264.5 42 138350.5 43 144613.0 44 146983.5 45 149180.0 46 156987.0 47 184760.0 48 244821.5 49 235763.5 50 165538.0 51 126884.5 52 102356.5 53 85641.0 54 72501.5 55 59965.0 56 48341.0 57 37968.5 58 29824.0 59 24136.0 60 19255.5 61 14587.0 62 10605.0 63 7318.0 64 4802.0 65 3023.5 66 1882.0 67 1111.0 68 621.5 69 376.5 70 239.5 71 155.0 72 114.0 73 81.0 74 58.5 75 55.0 76 35.5 77 18.5 78 19.0 79 16.0 80 17.0 81 15.5 82 12.5 83 12.5 84 8.5 85 4.5 86 4.5 87 4.0 88 4.0 89 3.5 90 5.0 91 5.0 92 1.5 93 0.5 94 1.0 95 1.5 96 1.0 97 2.5 98 3.5 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4330945058301076 2 0.8569319871587451 3 0.8507792820520279 4 0.8287973447162107 5 0.8386696397283524 6 0.8476469958158808 7 0.8276227373776556 8 0.8177504423655138 9 0.8148698577019143 10-11 0.8076823794636128 12-13 0.8107587320169715 14-15 0.8107866988583656 16-17 0.8383620044730166 18-19 0.8656017079909376 20-21 0.859043483684005 22-23 0.834278845629468 24-25 0.8269515331841955 26-27 0.8278884223709001 28-29 0.8486817689474652 30-31 0.8557993300822813 32-33 0.8505415639001774 34-35 0.8616863501957539 36-37 0.8715446617871986 38-39 0.8581485447593915 40-41 0.8676712542541062 42-43 0.8603719086502281 44-45 0.8461647532219899 46-47 0.8410188544054626 48-49 0.8461507698012929 50-51 0.8808016862886687 52-53 0.8493809399823193 54-55 0.8615045657266918 56-57 0.9091460800416593 58-59 0.8746070309198603 60-61 0.8885485013548535 62-63 0.862860957534309 64-65 0.851310652038517 66-67 0.8543450543297844 68-69 0.8733065727950313 70-71 0.8788999410738652 72-73 0.8836682875315711 74-75 0.8877234795337255 76-77 0.9017348670722045 78-79 0.9473627688067918 80-81 0.9735117655103404 82-83 0.939448152692242 84-85 0.901636983127325 86-87 0.8838360885799361 88-89 0.882479696772319 90-91 0.8961275153726735 92-93 0.8832767517520527 94-95 0.8771100632246385 96-97 0.8868844742919004 98-99 0.920137048709568 100-101 0.9075379866614947 102-103 0.8822699454618625 104-105 0.8715306783665016 106-107 0.8734184401606079 108-109 0.8715166949458045 110-111 0.8706497228625851 112-113 0.8810394044405192 114-115 0.8839060056834216 116-117 0.9109359578908862 118-119 0.9058320093364504 120-121 0.8845352596147903 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 3575663.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.5680165465035 #Duplication Level Percentage of deduplicated Percentage of total 1 77.03153291090781 38.95331830841315 2 13.14432712629611 13.293651032303933 3 4.067020493414021 6.169834788177873 4 1.7856356519229375 3.611842127698628 5 0.9854719737884567 2.491668153832508 6 0.6032136432143009 1.8301990494682463 7 0.4141142419075136 1.4658655085845333 8 0.29471885677939474 1.1922678420949624 9 0.22193012077801863 1.010030941770333 >10 1.2787148642565844 12.10297996000735 >50 0.1090867467301291 3.798773516126843 >100 0.05824995271568837 5.338334635199309 >500 0.003019668539931967 1.0542427813366713 >1k 0.002069032145626538 2.6311108244009924 >5k 6.151176649159978E-4 1.9727817065143611 >10k+ 2.795989385981808E-4 3.0830988240702473 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 29710 0.8308948578207733 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 26633 0.7448408868509141 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 20253 0.5664124387561132 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 19390 0.542277054632945 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 13044 0.3647994791455459 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 9505 0.2658248274515803 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 8533 0.23864105761644763 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 6574 0.18385401532526974 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6356 0.17775724390134082 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6256 0.17496055976192387 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5667 0.1584880901807581 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 5547 0.15513206921345776 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5523 0.15446086501999767 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5379 0.1504336398592373 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5242 0.1466021825882361 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5183 0.14495213894598008 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4606 0.12881527146154434 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4569 0.12778049832996005 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 4558 0.12747286307462422 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4482 0.12534738312866733 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 4437 0.1240888752659297 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 4296 0.12014555062935181 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4244 0.118691274876855 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 4162 0.11639799388253312 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4109 0.11491575128864213 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.7966841394169418E-5 0.0 0.0 0.0 6 0.0 2.7966841394169418E-5 0.0 0.0 0.0 7 0.0 2.7966841394169418E-5 0.0 0.0 0.0 8 0.0 2.7966841394169418E-5 0.0 0.0 0.0 9 8.390052418250825E-5 2.7966841394169418E-5 0.0 0.0 0.0 10-11 9.788394487959297E-5 5.5933682788338836E-5 0.0 0.0 0.0 12-13 1.1186736557667767E-4 5.5933682788338836E-5 0.0 2.7966841394169418E-5 0.0 14-15 1.1186736557667767E-4 6.991710348542355E-5 0.0 5.5933682788338836E-5 0.0 16-17 1.1186736557667767E-4 8.390052418250825E-5 0.0 1.678010483650165E-4 0.0 18-19 1.2585078627376238E-4 8.390052418250825E-5 0.0 2.936518346387789E-4 0.0 20-21 1.398342069708471E-4 8.390052418250825E-5 0.0 4.474694623067107E-4 0.0 22-23 1.398342069708471E-4 1.1186736557667767E-4 0.0 8.949389246134213E-4 0.0 24-25 1.398342069708471E-4 1.1186736557667767E-4 0.0 0.0013703752283143016 0.0 26-27 1.398342069708471E-4 1.1186736557667767E-4 0.0 0.0022373473115335535 0.0 28-29 1.398342069708471E-4 1.678010483650165E-4 0.0 0.004712412774917547 0.0 30-31 1.398342069708471E-4 1.678010483650165E-4 0.0 0.01054349920560187 0.0 32-33 1.398342069708471E-4 1.678010483650165E-4 0.0 0.020122142383104898 0.0 34-35 1.5381762766793178E-4 1.678010483650165E-4 0.0 0.029435100567363308 0.0 36-37 1.678010483650165E-4 1.678010483650165E-4 0.0 0.04062183712503108 0.0 38-39 1.678010483650165E-4 1.8178446906210122E-4 0.0 0.05940157112121584 0.0 40-41 1.678010483650165E-4 1.957678897591859E-4 0.0 0.10603627914599334 0.0 42-43 1.678010483650165E-4 1.957678897591859E-4 0.0 0.14022574275036545 0.0 44-45 1.678010483650165E-4 2.0975131045627063E-4 0.0 0.1777991941634321 0.0 46-47 1.678010483650165E-4 2.2373473115335534E-4 0.0 0.27218728386875385 0.0 48-49 1.8178446906210122E-4 2.2373473115335534E-4 0.0 0.31582953986435525 0.0 50-51 1.957678897591859E-4 2.5170157254752475E-4 0.0 0.37004326190695264 0.0 52-53 1.957678897591859E-4 2.5170157254752475E-4 0.0 0.43383562712705304 0.0 54-55 1.957678897591859E-4 2.5170157254752475E-4 0.0 0.5199734986210949 0.0 56-57 2.2373473115335532E-4 2.6568499324460947E-4 0.0 0.8273570523844109 0.0 58-59 2.5170157254752475E-4 2.796684139416942E-4 0.0 1.0566991352372974 0.0 60-61 3.0763525533586357E-4 2.796684139416942E-4 0.0 1.1809837783929862 0.0 62-63 3.0763525533586357E-4 3.0763525533586357E-4 0.0 1.3446457342316656 0.0 64-65 3.35602096730033E-4 3.35602096730033E-4 0.0 1.4958065119671513 0.0 66-67 3.35602096730033E-4 3.6356893812420244E-4 0.0 1.882518011345029 0.0 68-69 3.775523588212871E-4 3.6356893812420244E-4 0.0 2.2761093537058725 0.0 70-71 3.915357795183718E-4 3.6356893812420244E-4 0.0 2.378943429512233 0.0 72-73 3.915357795183718E-4 3.915357795183718E-4 0.0 2.485902614424234 0.0 74-75 3.915357795183718E-4 3.915357795183718E-4 0.0 2.816708397855167 0.0 76-77 3.915357795183718E-4 3.915357795183718E-4 0.0 3.1443679116292556 0.0 78-79 3.915357795183718E-4 3.915357795183718E-4 0.0 3.399914924868479 0.0 80-81 3.915357795183718E-4 3.915357795183718E-4 0.0 3.6072051532820626 0.0 82-83 4.1950262091254125E-4 3.915357795183718E-4 0.0 4.098232971060192 0.0 84-85 4.1950262091254125E-4 3.915357795183718E-4 0.0 5.173795740817857 0.0 86-87 4.1950262091254125E-4 3.915357795183718E-4 0.0 6.108111978114268 0.0 88-89 4.614528830037954E-4 3.915357795183718E-4 0.0 7.195183103105634 0.0 90-91 4.7543630370088007E-4 3.915357795183718E-4 0.0 8.332762343654869 0.0 92-93 4.7543630370088007E-4 4.1950262091254125E-4 0.0 9.439368307360063 0.0 94-95 4.7543630370088007E-4 4.1950262091254125E-4 0.0 10.445251691784152 0.0 96-97 4.7543630370088007E-4 4.1950262091254125E-4 0.0 11.51231254175799 0.0 98-99 4.7543630370088007E-4 4.1950262091254125E-4 0.0 12.683913444863233 0.0 100-101 4.7543630370088007E-4 4.1950262091254125E-4 0.0 13.554157648525603 0.0 102-103 4.7543630370088007E-4 4.474694623067107E-4 0.0 14.127184245271437 0.0 104-105 4.7543630370088007E-4 4.614528830037954E-4 0.0 14.764842212479197 0.0 106-107 4.7543630370088007E-4 4.7543630370088007E-4 0.0 15.73344579732486 0.0 108-109 4.894197243979648E-4 4.7543630370088007E-4 0.0 16.843631516728507 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3200 0.0 30.915714 2 ACCTGGG 3420 0.0 27.55119 3 GTACCCG 440 0.0 27.50364 1 GTACACG 465 0.0 24.785666 1 GTACATG 12180 0.0 22.71002 1 GAGTACT 8455 0.0 22.426882 12-13 TATAACG 490 0.0 22.302746 2 TAACGCA 490 0.0 22.276316 4 GGCGTCG 540 0.0 21.26947 8 TACATGG 11760 0.0 20.933279 2 AGTACTT 9200 0.0 20.798162 12-13 GTACTTT 9255 0.0 20.427229 14-15 GTACCTG 4980 0.0 20.366053 1 GTCGCCG 1790 0.0 20.074032 112-113 ACATGGG 11710 0.0 19.92011 3 TCTCGGG 1685 0.0 19.618942 114-115 TACCCGG 690 0.0 19.172537 2 GTATAAC 1255 0.0 18.826244 1 TAGACCG 155 0.0069660037 18.532864 5 CGGGCGT 625 0.0 18.384083 6 >>END_MODULE