FastQCFastQC Report
Wed 25 May 2016
SRR1003852_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003852_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3575663
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT551141.5413644965982531No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT180020.5034590787778378No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT136940.382977926051756No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC128920.3605485192536321No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC117740.3292815905749507No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG113740.31809485401728294No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC98830.2763962934985763No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA92360.25830174711654874No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA88440.24733874529003433No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT82940.23195698252324115No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA79530.2224202896078294No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79350.22191688646273433No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT77230.21598791608717038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65670.18365824743551057No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA63210.17677840445254486No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC59630.16676627523343224No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC56290.15742535020777965No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT51680.14453263632506755No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG45450.1271092941365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40210.11245466924595524No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG37950.10613416309087292No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT36720.10269424159939011No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA4000.041.555274
TACCTGG29450.031.298452
GTACCCG5100.027.960951
TATAACG5650.026.9096412
ACGTGCG2850.026.663529
ACCTGGG33950.026.0295943
GTCGTAC1309.973899E-625.5950261
ATAACGC7300.025.3825933
GTACCGG2751.8189894E-1224.1989351
GTACCTG40850.023.854081
GTATAAC9800.023.2817731
CTAACGC2252.8103386E-923.2276063
AATGCGT5100.021.4178456
TAACGCC3001.6734703E-1020.5797544
TACCCGG6900.019.280372
GAGTACT97800.019.27942512-13
AGTACTT100450.019.19634212-13
TCTAACG2603.2759453E-718.2739772
GTACTTT103650.017.96381614-15
TACGTGC4500.017.9413248
Produced by FastQC (version 0.11.5)