##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003852_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3575663 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.659278293284352 31.0 31.0 34.0 30.0 34.0 2 31.891304353905834 31.0 31.0 34.0 30.0 34.0 3 31.929064903487827 31.0 31.0 34.0 30.0 34.0 4 35.66680948400339 37.0 35.0 37.0 33.0 37.0 5 35.53910141979264 37.0 35.0 37.0 33.0 37.0 6 35.57595919973443 37.0 35.0 37.0 33.0 37.0 7 35.5052797201526 37.0 35.0 37.0 33.0 37.0 8 35.50273082222793 37.0 35.0 37.0 33.0 37.0 9 37.07340233125996 39.0 37.0 39.0 33.0 39.0 10-11 36.998401555180116 39.0 37.0 39.0 33.0 39.0 12-13 36.94861232728029 39.0 37.0 39.0 33.0 39.0 14-15 38.05209579314382 40.0 37.0 41.0 33.0 41.0 16-17 38.07315174836107 40.0 37.0 41.0 33.0 41.0 18-19 38.078856564502864 40.0 37.0 41.0 33.0 41.0 20-21 38.01530387511351 40.0 37.0 41.0 33.0 41.0 22-23 37.9345827612949 40.0 37.0 41.0 33.0 41.0 24-25 37.83549582273274 40.0 37.0 41.0 32.5 41.0 26-27 37.615032512851464 39.0 37.0 41.0 32.0 41.0 28-29 37.460462157647406 39.0 36.0 41.0 32.0 41.0 30-31 37.28129832145815 39.0 36.0 40.5 31.0 41.0 32-33 37.06619457706165 39.0 36.0 40.0 31.0 41.0 34-35 37.07508831229341 39.0 36.0 40.0 31.0 41.0 36-37 36.92282144598079 39.0 35.5 40.0 30.5 41.0 38-39 36.69183659086441 39.0 35.0 40.0 30.0 41.0 40-41 36.86536874420213 39.0 35.0 40.5 30.0 41.0 42-43 36.757763804922334 39.0 35.0 41.0 30.0 41.0 44-45 36.63907141137182 39.0 35.0 41.0 30.0 41.0 46-47 36.40677896658606 39.0 35.0 41.0 30.0 41.0 48-49 36.18904088556444 39.0 35.0 40.5 29.5 41.0 50-51 35.95846742268496 39.0 35.0 40.0 28.5 41.0 52-53 35.79371559903716 39.0 35.0 40.0 28.0 41.0 54-55 35.60011402081236 38.0 34.5 40.0 28.0 41.0 56-57 35.36244467110016 38.0 34.0 40.0 27.5 41.0 58-59 35.10500486203537 38.0 34.0 40.0 27.0 41.0 60-61 34.66161310503814 38.0 34.0 40.0 26.0 41.0 62-63 34.450913159321786 37.0 33.0 40.0 26.0 41.0 64-65 34.013895464980905 37.0 33.0 39.5 25.5 41.0 66-67 33.67884319635267 36.0 32.5 39.0 25.0 41.0 68-69 33.390489539981814 36.0 32.0 39.0 25.0 40.5 70-71 32.96361905470398 35.0 32.0 38.5 23.5 40.0 72-73 32.594684817892514 35.0 31.5 38.0 23.0 40.0 74-75 32.21876921846382 35.0 31.0 37.0 22.0 39.5 76-77 31.22490654180777 34.0 30.5 36.0 21.5 39.0 78-79 31.60808149425715 34.5 31.0 36.0 22.0 39.0 80-81 31.416317477346162 34.5 31.0 36.0 21.0 38.5 82-83 31.116985017883394 34.0 31.0 35.5 20.5 37.0 84-85 30.78321027457006 34.0 31.0 35.0 20.0 37.0 86-87 30.449207461665154 34.0 31.0 35.0 18.5 36.0 88-89 30.150984586634703 34.0 30.0 35.0 18.0 36.0 90-91 29.872074773265823 34.0 30.0 35.0 14.5 36.0 92-93 29.62418997539757 34.0 30.0 35.0 8.5 35.0 94-95 29.293431455928594 34.0 30.0 35.0 3.5 35.0 96-97 28.900815317327165 34.0 29.0 35.0 2.0 35.0 98-99 28.532271777289974 34.0 29.0 35.0 2.0 35.0 100-101 27.54018849091763 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.030718713395497588 1101 2 0.05799092231885794 1101 3 0.08851965262600459 1101 4 0.11627625849379797 1101 5 0.038142361896447596 1101 6 0.0806009869191584 1101 7 0.06262902777751123 1101 8 0.10368551353469968 1101 9 0.1796477604783533 1101 10-11 0.1911161348933348 1101 12-13 0.1258999284503588 1101 14-15 0.05238151727497353 1101 16-17 0.12098733176527077 1101 18-19 0.0403943338394086 1101 20-21 -0.003025326943479456 1101 22-23 0.00546600595888691 1101 24-25 0.07620215382196704 1101 26-27 0.2157430329704937 1101 28-29 0.05822786570060856 1101 30-31 0.16203834528044325 1101 32-33 0.1495781829408216 1101 34-35 0.1458502636293062 1101 36-37 0.11025751282220853 1101 38-39 -0.004351231188408633 1101 40-41 0.09661434193059648 1101 42-43 0.02204088304543461 1101 44-45 0.05320654029282679 1101 46-47 0.017310452263394893 1101 48-49 0.02555843380978473 1101 50-51 0.005425421653797002 1101 52-53 -0.013611776230369799 1101 54-55 0.07376819067816598 1101 56-57 0.122121013805355 1101 58-59 -0.03478924427428609 1101 60-61 7.884264484232517E-4 1101 62-63 -0.12961034215973655 1101 64-65 -0.10703135563768029 1101 66-67 -0.16618172404175624 1101 68-69 -0.2740328421457505 1101 70-71 -0.1960773691270603 1101 72-73 -0.12604851591058974 1101 74-75 -0.20445037898021923 1101 76-77 -0.14426512736608643 1101 78-79 -0.11197630364634747 1101 80-81 -0.029207913950298092 1101 82-83 0.01860347941801166 1101 84-85 -0.07903954266003765 1101 86-87 -0.3187207458142254 1101 88-89 -0.1981728079508578 1101 90-91 -0.14625174805164676 1101 92-93 0.08200255007005453 1101 94-95 -0.040305976786321196 1101 96-97 0.08987421236525961 1101 98-99 -0.16899207510871506 1101 100-101 -0.0511511866308858 1102 1 -0.066862836995913 1102 2 -0.07803565751668629 1102 3 -0.09984742361728394 1102 4 -0.04264250383355517 1102 5 -0.09940886600517729 1102 6 -0.0496145507461776 1102 7 -0.17970563668117023 1102 8 -0.16282078123384736 1102 9 -0.11767769664132999 1102 10-11 -0.06559871464061473 1102 12-13 -0.160347800673172 1102 14-15 -0.11016815467186092 1102 16-17 -0.09388774610617645 1102 18-19 -0.22089215477456747 1102 20-21 -0.2112413739863328 1102 22-23 -0.30658033806735574 1102 24-25 -0.2628544931713179 1102 26-27 -0.36614698812455515 1102 28-29 -0.24457393578679643 1102 30-31 -0.3368213181047466 1102 32-33 -0.313653627089991 1102 34-35 -0.4890918116986285 1102 36-37 -0.4950142603460108 1102 38-39 -0.4653202460575443 1102 40-41 -0.32713355404596456 1102 42-43 -0.18162869359173328 1102 44-45 -0.28386245151977363 1102 46-47 -0.3189506224223848 1102 48-49 -0.3041635566051042 1102 50-51 -0.2753815584156669 1102 52-53 -0.2907250456111399 1102 54-55 -0.2971874057226529 1102 56-57 -0.3488329958164229 1102 58-59 -0.3938008438971181 1102 60-61 -0.4297307006451234 1102 62-63 -0.5087692645673698 1102 64-65 -0.5910403217969957 1102 66-67 -0.5820924861615495 1102 68-69 -0.6563006453107434 1102 70-71 -0.5405616313613848 1102 72-73 -0.5804676446177233 1102 74-75 -0.5016256817722393 1102 76-77 -0.5574908556373579 1102 78-79 -0.5131355958378236 1102 80-81 -0.5436083358190906 1102 82-83 -0.5561486359434014 1102 84-85 -0.6019185109277245 1102 86-87 -0.6678227494926574 1102 88-89 -0.6900322562095624 1102 90-91 -0.5114697176166878 1102 92-93 -0.32658721024107606 1102 94-95 -0.43833700914530027 1102 96-97 -0.47751865230752344 1102 98-99 -0.6633883595583718 1102 100-101 -0.6760835809984407 1103 1 -0.03818606886284215 1103 2 -0.09313119625292998 1103 3 -0.06834213537862155 1103 4 -0.03068021592718395 1103 5 -0.022723498275837528 1103 6 -0.005317357107841758 1103 7 -0.023692195437455155 1103 8 -0.06707462573630352 1103 9 -0.04052053194883598 1103 10-11 -0.04435812015144336 1103 12-13 0.007521481035446698 1103 14-15 0.07720874408474288 1103 16-17 -0.0888955485816254 1103 18-19 0.02814992240812586 1103 20-21 0.0803054611916636 1103 22-23 0.04757461476813063 1103 24-25 -0.08651345925687082 1103 26-27 -8.550796001927097E-4 1103 28-29 -0.0696399305222073 1103 30-31 0.009936713022902666 1103 32-33 0.06418589635400451 1103 34-35 0.003053605088787492 1103 36-37 -0.0720522408050357 1103 38-39 0.0125941566294685 1103 40-41 0.0861437421640332 1103 42-43 0.07357876464512003 1103 44-45 0.18521815267316555 1103 46-47 0.3104735156672973 1103 48-49 0.2502228725453861 1103 50-51 0.24062983898956247 1103 52-53 0.2776456669332177 1103 54-55 0.14240193034468973 1103 56-57 0.23308234272720796 1103 58-59 0.06829133845636193 1103 60-61 0.12596437436475583 1103 62-63 -0.1313797546011557 1103 64-65 -0.09988959489815841 1103 66-67 -0.1909109350357383 1103 68-69 -0.15088328295633602 1103 70-71 -0.11282643950393378 1103 72-73 -0.014711515735911007 1103 74-75 0.08831966846246075 1103 76-77 0.04508150615490791 1103 78-79 -0.03396173868666352 1103 80-81 -0.03278703235603686 1103 82-83 -0.13867673916902845 1103 84-85 -0.1642866828986982 1103 86-87 -0.04412534686379388 1103 88-89 0.016468995239769413 1103 90-91 0.0025837551261886915 1103 92-93 -0.10704196609076178 1103 94-95 -0.2652876919891227 1103 96-97 -0.12026645686251669 1103 98-99 0.03396995836136085 1103 100-101 0.0617059273250824 1104 1 -0.10644983077045111 1104 2 -0.11710205520871142 1104 3 -0.059640188416310025 1104 4 -0.04445713366804682 1104 5 -0.08050065360197323 1104 6 -0.08195522665342736 1104 7 -0.049360036802646334 1104 8 -0.09234440870396554 1104 9 -0.14982586045556445 1104 10-11 -0.13220755989342337 1104 12-13 -0.0589837440366523 1104 14-15 -0.023380110486272088 1104 16-17 -0.017475697018305425 1104 18-19 -0.09908826223428235 1104 20-21 -0.16125330431322027 1104 22-23 -0.20464179551947126 1104 24-25 -0.177161869909348 1104 26-27 -0.09738417658034138 1104 28-29 -0.056846852860175545 1104 30-31 -0.12414934515405918 1104 32-33 -0.08029677203261798 1104 34-35 -2.5039556882688885E-4 1104 36-37 -0.058846932088492565 1104 38-39 -0.0646493685277747 1104 40-41 -0.09684062384921788 1104 42-43 -0.08065237257918056 1104 44-45 -0.029284527505105018 1104 46-47 -0.059000662009374594 1104 48-49 0.00984874790022161 1104 50-51 0.14033271567816996 1104 52-53 0.04476591445479983 1104 54-55 -0.11367694770655845 1104 56-57 -0.16189968634374452 1104 58-59 -0.11829468622158146 1104 60-61 -0.03217255385872164 1104 62-63 -0.1789655455591017 1104 64-65 -0.1610655788413098 1104 66-67 -0.10440931497220873 1104 68-69 -0.25505968795624057 1104 70-71 -0.18023356092907505 1104 72-73 0.011425698259877493 1104 74-75 -0.14018456606975604 1104 76-77 -0.14260628155002664 1104 78-79 -0.031170047368160425 1104 80-81 -0.12587471832706498 1104 82-83 -0.15873894038364966 1104 84-85 -0.10775691464998971 1104 86-87 -0.011778479845581558 1104 88-89 -0.07472361323001664 1104 90-91 -0.17685251842361183 1104 92-93 -0.3475611994170187 1104 94-95 -0.25490857212658113 1104 96-97 -0.025987977810220997 1104 98-99 -0.26416708453179893 1104 100-101 -0.1859464364679546 1105 1 0.03697614318784659 1105 2 0.07804728072073885 1105 3 0.12069169585278416 1105 4 0.13112132247155017 1105 5 0.05506250356967968 1105 6 0.09581644180162385 1105 7 0.1276015295369035 1105 8 0.16403714406281722 1105 9 0.20319701071687746 1105 10-11 0.07065177339489992 1105 12-13 0.030913496464187062 1105 14-15 0.14496993860544194 1105 16-17 0.2141362655488237 1105 18-19 0.1385051985598622 1105 20-21 0.0240042584163902 1105 22-23 0.13221893146427277 1105 24-25 0.08729424097278837 1105 26-27 0.1960399690224861 1105 28-29 0.14759252745907503 1105 30-31 0.1330279863766819 1105 32-33 0.1918118577459751 1105 34-35 0.1855303210991437 1105 36-37 0.1685069238317638 1105 38-39 0.3463486189488023 1105 40-41 0.3662241159232451 1105 42-43 0.332002756350235 1105 44-45 0.2970936898083423 1105 46-47 0.4292480828653069 1105 48-49 0.3423970727416972 1105 50-51 0.20368005110545084 1105 52-53 0.354943014739284 1105 54-55 0.3715047493744734 1105 56-57 0.3460555105086982 1105 58-59 0.228327645415753 1105 60-61 0.30280019254141166 1105 62-63 0.23863165781205709 1105 64-65 0.1314805860239474 1105 66-67 0.11862669815287319 1105 68-69 0.18757158796643125 1105 70-71 0.42954197475687295 1105 72-73 0.38080413438379423 1105 74-75 0.16751629691402314 1105 76-77 0.20366625681731065 1105 78-79 0.32346170259453544 1105 80-81 0.39912312021627017 1105 82-83 0.336141849359322 1105 84-85 0.30100422425410045 1105 86-87 0.4273898760142778 1105 88-89 0.38329268178454257 1105 90-91 0.3760825916863233 1105 92-93 0.2587927947772535 1105 94-95 0.3948479901150783 1105 96-97 0.4017570055376787 1105 98-99 0.319154997383265 1105 100-101 0.2848364431371344 1106 1 -0.007075421701586038 1106 2 -0.008502996779398586 1106 3 -0.007590637156557278 1106 4 -0.023345429924788164 1106 5 -0.018610568017997764 1106 6 -0.04794055686452481 1106 7 0.01797457596293839 1106 8 0.07004668238148781 1106 9 -0.07925857881227216 1106 10-11 -0.12231076463851309 1106 12-13 -0.094495720036889 1106 14-15 0.02188804671488498 1106 16-17 -0.03107270096682413 1106 18-19 0.04976958729077552 1106 20-21 0.00989504940774566 1106 22-23 -0.06666963235634427 1106 24-25 -0.009155172485684204 1106 26-27 0.06676263827022666 1106 28-29 0.03567290812195978 1106 30-31 0.06504452986003173 1106 32-33 0.12165861297279434 1106 34-35 0.14367356169797318 1106 36-37 0.09054290854992786 1106 38-39 0.3214865450211448 1106 40-41 0.046681775808842474 1106 42-43 0.05993496704272161 1106 44-45 0.1810470767167729 1106 46-47 0.11904604726965573 1106 48-49 0.034227719079929386 1106 50-51 0.15885044606170595 1106 52-53 0.20417571203179818 1106 54-55 0.09363861135716434 1106 56-57 0.1348986916268302 1106 58-59 0.18474911432905827 1106 60-61 0.2093793507153947 1106 62-63 0.21803194252429847 1106 64-65 0.0549492213811007 1106 66-67 0.05327553012423181 1106 68-69 0.19901631313621948 1106 70-71 0.22546630667785905 1106 72-73 0.15748244234566044 1106 74-75 0.25579739307822535 1106 76-77 0.09283890086092228 1106 78-79 0.24180201781674882 1106 80-81 0.2538995765971741 1106 82-83 0.21328948738435827 1106 84-85 0.1665415306806146 1106 86-87 0.3467206867271919 1106 88-89 0.21291089249436013 1106 90-91 0.19588449088921678 1106 92-93 0.2261393185203424 1106 94-95 0.2330440107017573 1106 96-97 0.3459162931828388 1106 98-99 0.07868932348869606 1106 100-101 0.26106357445661743 1107 1 -0.08496423218578286 1107 2 -0.06193463593632487 1107 3 -0.07772762808673406 1107 4 -0.12675203190339346 1107 5 -0.031151624137585543 1107 6 -0.02430351938280495 1107 7 -0.04225987842967527 1107 8 -0.019395896207036856 1107 9 0.013638159812167316 1107 10-11 -0.0050848264812373145 1107 12-13 -0.045096899893700026 1107 14-15 0.011129519066322757 1107 16-17 -0.045937020309658294 1107 18-19 -0.03981695956649389 1107 20-21 -0.037045795630270106 1107 22-23 -0.07339066786384052 1107 24-25 -0.11357000168467835 1107 26-27 -0.06148424160847554 1107 28-29 -0.08458984385396917 1107 30-31 -0.0234419448513421 1107 32-33 -0.01583081407662945 1107 34-35 -0.09196834446347424 1107 36-37 -0.036198595255250154 1107 38-39 0.04758925029455696 1107 40-41 -0.004712020363008662 1107 42-43 0.10713463144028879 1107 44-45 -0.0024274509648520848 1107 46-47 0.029644268677394336 1107 48-49 -0.047817838159950554 1107 50-51 0.21257550369394806 1107 52-53 -0.0077143954249692115 1107 54-55 -0.13217966222424593 1107 56-57 -0.2311668730684815 1107 58-59 -0.06505829890445369 1107 60-61 -0.08530212712877017 1107 62-63 -0.2227419201713161 1107 64-65 -0.2335704684814104 1107 66-67 -0.21334264985085127 1107 68-69 -0.11437987136316252 1107 70-71 -0.06959135791597504 1107 72-73 -0.08693281102950579 1107 74-75 -0.11112730810224747 1107 76-77 -0.16013239930734713 1107 78-79 -0.28512921495783417 1107 80-81 -0.14620603608037186 1107 82-83 -0.17951121847355012 1107 84-85 -0.19555978415768038 1107 86-87 -0.02930843476441325 1107 88-89 -0.23398476699713555 1107 90-91 -9.34382898982733E-5 1107 92-93 -0.049585168990361694 1107 94-95 -0.028484953880610675 1107 96-97 0.0562415320605254 1107 98-99 0.054267829691195146 1107 100-101 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-0.4757738677117871 2316 52-53 -0.3899590182548778 2316 54-55 -0.5577513337976114 2316 56-57 -0.5570232450667305 2316 58-59 -0.8807640533238654 2316 60-61 -0.5660594356700557 2316 62-63 -0.6329653361821741 2316 64-65 -0.453910566831766 2316 66-67 -0.5583627694281148 2316 68-69 -0.4405086335656492 2316 70-71 -0.607949387059314 2316 72-73 -0.5359915912702249 2316 74-75 -0.5193265914425567 2316 76-77 -0.5063386096107507 2316 78-79 -0.4756652448672263 2316 80-81 -0.4186768268448837 2316 82-83 -0.4275468976286163 2316 84-85 -0.5046603418473907 2316 86-87 -0.32690647301383535 2316 88-89 -0.3050749384937781 2316 90-91 -0.5581539079489737 2316 92-93 -0.39491536312080555 2316 94-95 -0.6540082348848806 2316 96-97 -0.4764226818381587 2316 98-99 -0.626855326239685 2316 100-101 -1.1123567019947664 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 16.0 4 38.0 5 65.0 6 168.0 7 433.0 8 998.0 9 1751.0 10 3070.0 11 4714.0 12 6581.0 13 9229.0 14 12122.0 15 16671.0 16 21062.0 17 26766.0 18 23962.0 19 17918.0 20 16583.0 21 17255.0 22 18927.0 23 21258.0 24 25499.0 25 31290.0 26 39628.0 27 50253.0 28 64264.0 29 80460.0 30 100616.0 31 124454.0 32 154091.0 33 194916.0 34 252578.0 35 336774.0 36 479819.0 37 663354.0 38 627504.0 39 130543.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.550419697817574 21.38623391158366 15.11675993284835 20.946586457750417 2 14.925237884543419 22.462196620643866 39.518284848386536 23.094280646426178 3 21.145329955033095 26.3207210373053 29.790520017995714 22.743428989665894 4 12.9838578188157 17.379657982309855 39.57646456055842 30.060019638316028 5 12.638663095487464 39.33340474200169 34.875937693233396 13.151994469277446 6 29.212954598719215 39.55908699623507 17.17193017390521 14.056028231140505 7 27.11760787233245 33.70838742588086 22.020621635937626 17.153383065849066 8 24.84129516679844 35.18172713703724 19.58811554668323 20.38886214948109 9 24.39446894184379 19.828350714259145 22.08616975369323 33.69101059020383 10-11 24.510956323490092 27.24612182617324 27.84597046916199 20.396951381174677 12-13 26.599570485249867 24.744236243739973 27.638468725939774 21.017724545070383 14-15 22.18161813823726 27.087725097812683 27.321351236420227 23.409305527529835 16-17 20.602165248794417 30.09686595185285 28.078359174228666 21.22260962512407 18-19 20.51822277434982 30.735348940881735 29.182755757463717 19.563672527304725 20-21 22.132060127642227 27.97374432815814 28.631674487847274 21.262521056352355 22-23 21.216876422638265 28.248831055946827 28.7933734247327 21.74091909668221 24-25 22.331019450099184 28.54006375880501 28.78631179728067 20.342604993815133 26-27 22.112093022410242 27.530960552207283 30.081824491353615 20.275121934028856 28-29 20.93631600116131 29.723814483796783 29.394735178635063 19.94513433640685 30-31 21.68600342659292 28.465474580042383 28.68692629356959 21.161595699795107 32-33 21.53261286055104 28.833427028357427 28.82698025132641 20.806979859765118 34-35 22.51437016493228 27.857150149082198 29.344280245730765 20.284199440254756 36-37 22.324359705951462 27.55436485291979 29.788988285052532 20.33228715607622 38-39 21.582308327440995 28.540731537587156 29.94671162744905 19.9302485075228 40-41 21.344287541532093 28.719022769615638 29.089724430228177 20.84696525862409 42-43 20.78638283305781 29.47307953797659 28.216081884674253 21.524455744291338 44-45 21.816331684501588 27.959486114882747 28.366361706905824 21.857820493709838 46-47 22.551477537792657 28.895031031321057 28.114126574900894 20.43936485598539 48-49 22.46879809422756 28.688581670028746 28.32429957744899 20.5183206582947 50-51 21.099191954051598 28.916693211860288 29.25556742903344 20.72854740505467 52-53 21.77137498696046 30.023550877138028 27.474303366956004 20.730770768945508 54-55 21.021891604438114 29.18532870687198 27.8992874887818 21.893492199908103 56-57 22.183868329353366 28.318918810829512 28.029234662478675 21.467978197338446 58-59 23.355905890795658 27.902919591364324 27.683113678908295 21.058060838931723 60-61 21.826020644548127 28.797342004988913 29.07275741110637 20.303879939356587 62-63 21.2639041271666 28.634568735179826 28.68645463375338 21.415072503900184 64-65 21.034592891776498 30.16823941846259 28.050092283431916 20.747075406328992 66-67 21.72073670210571 28.196119332884546 27.892090336767676 22.191053628242067 68-69 21.359113603931085 29.02726183908866 27.678062648183065 21.935561908797187 70-71 22.28152459616766 28.853199636373656 27.59559559615549 21.26968017130319 72-73 21.983545429197328 27.873026624712676 28.789262299047756 21.35416564704224 74-75 21.180033070794575 29.92684572439373 28.02240144308922 20.870719761722476 76-77 21.728361984896228 28.557025648110574 27.492314012813846 22.22229835417935 78-79 21.532510194612858 29.194837432946002 27.621157251116784 21.651495121324352 80-81 22.215298873327512 28.73853848602021 27.537572063578715 21.508590577073562 82-83 21.58623449693106 28.08680516032971 28.582349622992993 21.74461071974624 84-85 21.157996006331697 28.633301264661633 28.43904342135438 21.769659307652294 86-87 21.631795956916207 28.658529934666188 27.450703189389753 22.258970919027853 88-89 20.981115949685414 28.93032704703995 27.291945577645322 22.79661142562932 90-91 21.467431354688628 26.781956241401943 28.43742545088841 23.31318695302102 92-93 21.453755569246876 28.25551513104003 28.398761292660968 21.891968007052117 94-95 20.982152096201595 28.33776328720476 28.54943062227987 22.13065399431377 96-97 19.969675435144783 28.661426219408003 28.971467837027625 22.397430508419593 98-99 20.606321716122107 29.598242889460717 27.40392427320065 22.39151112121652 100-101 19.43605312700271 28.48334995282637 28.009348307517534 24.071248612653385 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 170.0 1 124.0 2 119.0 3 172.0 4 175.0 5 173.0 6 282.5 7 572.0 8 914.0 9 1147.5 10 1250.0 11 1338.5 12 1523.5 13 1763.5 14 2052.5 15 2381.5 16 2729.0 17 3053.0 18 3363.5 19 3788.5 20 4298.0 21 4737.0 22 5347.0 23 6290.0 24 7698.0 25 9679.0 26 12497.5 27 17133.5 28 24176.0 29 33807.5 30 45408.5 31 57220.5 32 68726.5 33 79369.0 34 88275.5 35 96115.0 36 104001.0 37 113653.5 38 123206.5 39 127395.5 40 129811.0 41 133458.5 42 137603.5 43 143423.0 44 146377.0 45 149011.5 46 156338.0 47 187037.5 48 253898.5 49 243463.0 50 169544.0 51 131494.5 52 105077.0 53 86873.0 54 73579.5 55 61964.0 56 50552.0 57 39437.0 58 30860.5 59 24954.0 60 19945.0 61 15004.5 62 10807.5 63 7443.5 64 4827.5 65 2976.5 66 1721.5 67 955.0 68 519.5 69 271.0 70 143.0 71 87.0 72 59.0 73 38.5 74 25.5 75 18.5 76 7.0 77 3.0 78 3.5 79 2.0 80 1.5 81 1.5 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.046508857238503745 2 0.005285733023498019 3 0.04136295842197656 4 0.0 5 0.0 6 0.0010347731315842686 7 2.7966841394169415E-5 8 0.0 9 0.0 10-11 3.0763525533586357E-4 12-13 0.0 14-15 0.002195397049442299 16-17 0.0 18-19 0.0 20-21 0.012179559427160782 22-23 0.0 24-25 0.0 26-27 0.00745316323154615 28-29 0.010767233936755227 30-31 0.010949018405817327 32-33 0.006516274044841474 34-35 0.008012500059429538 36-37 0.020779363155867876 38-39 0.011158769716273598 40-41 0.011634206019974476 42-43 0.0 44-45 0.0 46-47 4.1950262091254125E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.001244524442040539 58-59 2.3771815185044004E-4 60-61 0.009410842129138008 62-63 0.014277072531723486 64-65 0.04529229963785737 66-67 0.023478163350405225 68-69 0.0016919939043472496 70-71 4.055192002154566E-4 72-73 0.0 74-75 1.3983420697084707E-5 76-77 0.0 78-79 0.0 80-81 2.7966841394169415E-5 82-83 0.0 84-85 8.390052418250825E-5 86-87 7.271378762484048E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.398342069708471E-4 96-97 3.915357795183718E-4 98-99 5.593368278833884E-4 100-101 0.0011885907592522002 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3575663.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.776488541932196 #Duplication Level Percentage of deduplicated Percentage of total 1 74.34466909685992 34.77582562163009 2 14.396026536684767 13.467911406851737 3 4.580329537763187 6.4275519642436 4 2.094217103774991 3.9184048943619723 5 1.153783934823355 2.6984980503565055 6 0.678785710776148 1.9050727213528673 7 0.47092809973535343 1.5419854002941265 8 0.3282038509631394 1.228177893919625 9 0.2575483139395409 1.0842485180388206 >10 1.482278487792125 12.877823136097799 >50 0.13302775475428164 4.330605139729823 >100 0.07238047903382099 6.153690902060778 >500 0.004298616056248505 1.3589262295148326 >1k 0.002447823029907138 2.300539784892945 >5k 7.164360087533087E-4 2.4999104044147864 >10k+ 3.5821800437665436E-4 3.430827932239561 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 55114 1.5413644965982531 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 18002 0.5034590787778378 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 13694 0.382977926051756 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12892 0.3605485192536321 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 11774 0.3292815905749507 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11374 0.31809485401728294 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9883 0.2763962934985763 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9236 0.25830174711654874 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8844 0.24733874529003433 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 8294 0.23195698252324115 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7953 0.2224202896078294 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7935 0.22191688646273433 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7723 0.21598791608717038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6567 0.18365824743551057 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6321 0.17677840445254486 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5963 0.16676627523343224 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5629 0.15742535020777965 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 5168 0.14453263632506755 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4545 0.1271092941365 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4021 0.11245466924595524 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3795 0.10613416309087292 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3672 0.10269424159939011 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.5933682788338836E-5 0.0 5 0.0 0.0 0.0 5.5933682788338836E-5 0.0 6 0.0 0.0 0.0 5.5933682788338836E-5 0.0 7 0.0 0.0 0.0 5.5933682788338836E-5 0.0 8 0.0 0.0 0.0 5.5933682788338836E-5 0.0 9 0.0 0.0 0.0 5.5933682788338836E-5 0.0 10-11 1.3983420697084709E-5 0.0 0.0 5.5933682788338836E-5 0.0 12-13 2.7966841394169418E-5 2.7966841394169418E-5 0.0 8.390052418250825E-5 0.0 14-15 2.7966841394169418E-5 2.7966841394169418E-5 0.0 9.788394487959297E-5 0.0 16-17 2.7966841394169418E-5 2.7966841394169418E-5 0.0 2.0975131045627063E-4 0.0 18-19 4.1950262091254125E-5 2.7966841394169418E-5 0.0 3.2161867603294834E-4 0.0 20-21 5.5933682788338836E-5 2.7966841394169418E-5 0.0 5.313699864892189E-4 0.0 22-23 5.5933682788338836E-5 2.7966841394169418E-5 0.0 9.648560280988449E-4 0.0 24-25 5.5933682788338836E-5 2.7966841394169418E-5 0.0 0.0014542757524968098 0.0 26-27 5.5933682788338836E-5 4.1950262091254125E-5 0.0 0.0023212478357160617 0.0 28-29 5.5933682788338836E-5 5.5933682788338836E-5 0.0 0.00478232987840297 0.0 30-31 5.5933682788338836E-5 5.5933682788338836E-5 0.0 0.010865117881634818 0.0 32-33 5.5933682788338836E-5 6.991710348542355E-5 0.0 0.020891230521444554 0.0 34-35 1.398342069708471E-4 8.390052418250825E-5 0.0 0.03073555869219219 0.0 36-37 1.398342069708471E-4 8.390052418250825E-5 0.0 0.04243968181565209 0.0 38-39 1.5381762766793178E-4 8.390052418250825E-5 0.0 0.06224020552272404 0.0 40-41 1.678010483650165E-4 8.390052418250825E-5 0.0 0.1107347085002138 0.0 42-43 1.678010483650165E-4 8.390052418250825E-5 0.0 0.14633649759499148 0.0 44-45 1.678010483650165E-4 8.390052418250825E-5 0.0 0.1859095781677412 0.0 46-47 1.678010483650165E-4 8.390052418250825E-5 0.0 0.2819616949360161 0.0 48-49 1.678010483650165E-4 8.390052418250825E-5 0.0 0.3267925416908697 0.0 50-51 1.678010483650165E-4 1.1186736557667767E-4 0.0 0.38209697054783964 0.0 52-53 1.678010483650165E-4 1.1186736557667767E-4 0.0 0.447357594941134 0.0 54-55 1.678010483650165E-4 1.1186736557667767E-4 0.0 0.5356209463811327 0.0 56-57 1.678010483650165E-4 1.1186736557667767E-4 0.0 0.8487237192095565 0.0 58-59 1.678010483650165E-4 1.398342069708471E-4 0.0 1.083589253237791 0.0 60-61 1.678010483650165E-4 1.398342069708471E-4 0.0 1.21055871316732 0.0 62-63 1.957678897591859E-4 1.398342069708471E-4 0.0 1.3778004247044535 0.0 64-65 1.957678897591859E-4 1.398342069708471E-4 0.0 1.5328346099730314 0.0 66-67 1.957678897591859E-4 1.398342069708471E-4 0.0 1.9267196041685137 0.0 68-69 1.957678897591859E-4 1.398342069708471E-4 0.0 2.3304908767968344 0.0 70-71 1.957678897591859E-4 1.398342069708471E-4 0.0 2.4381492327436898 0.0 72-73 1.957678897591859E-4 1.398342069708471E-4 0.0 2.549974648058276 0.0 74-75 1.957678897591859E-4 1.398342069708471E-4 0.0 2.891981151467574 0.0 76-77 1.957678897591859E-4 1.398342069708471E-4 0.0 3.23236557807601 0.0 78-79 1.957678897591859E-4 1.398342069708471E-4 0.0 3.4989874605073243 0.0 80-81 1.957678897591859E-4 1.398342069708471E-4 0.0 3.715241061587739 0.0 82-83 1.957678897591859E-4 1.398342069708471E-4 0.0 4.221510807925691 0.0 84-85 1.957678897591859E-4 1.398342069708471E-4 0.0 5.333766073592505 0.0 86-87 1.957678897591859E-4 1.398342069708471E-4 0.0 6.297531394876978 0.0 88-89 1.957678897591859E-4 1.398342069708471E-4 0.0 7.414373222532436 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 400 0.0 41.55527 4 TACCTGG 2945 0.0 31.29845 2 GTACCCG 510 0.0 27.96095 1 TATAACG 565 0.0 26.909641 2 ACGTGCG 285 0.0 26.66352 9 ACCTGGG 3395 0.0 26.029594 3 GTCGTAC 130 9.973899E-6 25.595026 1 ATAACGC 730 0.0 25.382593 3 GTACCGG 275 1.8189894E-12 24.198935 1 GTACCTG 4085 0.0 23.85408 1 GTATAAC 980 0.0 23.281773 1 CTAACGC 225 2.8103386E-9 23.227606 3 AATGCGT 510 0.0 21.417845 6 TAACGCC 300 1.6734703E-10 20.579754 4 TACCCGG 690 0.0 19.28037 2 GAGTACT 9780 0.0 19.279425 12-13 AGTACTT 10045 0.0 19.196342 12-13 TCTAACG 260 3.2759453E-7 18.273977 2 GTACTTT 10365 0.0 17.963816 14-15 TACGTGC 450 0.0 17.941324 8 >>END_MODULE