##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003851_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2857671 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.92672529482925 31.0 31.0 34.0 28.0 34.0 2 30.854591728718948 31.0 31.0 34.0 27.0 34.0 3 30.939605363948473 31.0 31.0 34.0 27.0 34.0 4 34.392970009493744 37.0 35.0 37.0 30.0 37.0 5 34.35684268762919 37.0 35.0 37.0 30.0 37.0 6 34.47509387889649 37.0 35.0 37.0 30.0 37.0 7 34.51866432489954 37.0 35.0 37.0 31.0 37.0 8 34.50115811092319 37.0 35.0 37.0 30.0 37.0 9 35.965188434917806 39.0 35.0 39.0 30.0 39.0 10-11 35.79519283360471 39.0 35.0 39.0 30.0 39.0 12-13 35.665092132719266 39.0 35.0 39.0 30.0 39.0 14-15 36.62296744446789 39.0 36.0 41.0 30.0 41.0 16-17 36.72923212644143 39.0 36.0 41.0 31.0 41.0 18-19 36.727825561444966 39.0 36.0 41.0 30.5 41.0 20-21 36.599396851492 39.0 36.0 41.0 30.0 41.0 22-23 36.523637955523924 39.0 36.0 41.0 30.0 41.0 24-25 36.29010862342096 39.0 36.0 41.0 29.0 41.0 26-27 36.106489865348394 39.0 35.5 41.0 28.0 41.0 28-29 35.96405849378742 39.0 35.0 40.0 27.0 41.0 30-31 35.760551512053 38.5 35.0 40.0 27.0 41.0 32-33 35.526238149878 38.0 35.0 40.0 27.0 41.0 34-35 35.50577025836774 38.0 35.0 40.0 27.0 41.0 36-37 35.35018061911256 38.0 35.0 40.0 25.5 41.0 38-39 35.36062601328145 38.0 34.5 40.0 26.0 41.0 40-41 35.554715010930224 39.0 35.0 40.0 27.0 41.0 42-43 35.41708265227173 39.0 35.0 40.0 26.0 41.0 44-45 35.213685200290726 38.5 35.0 40.0 25.0 41.0 46-47 34.95787793626348 38.0 34.0 40.0 24.0 41.0 48-49 34.68361228426925 38.0 34.0 40.0 23.5 41.0 50-51 33.89134560976403 37.5 33.5 39.5 23.0 40.5 52-53 34.05799215515012 38.0 33.5 39.5 23.0 40.5 54-55 34.42964323044885 38.0 34.0 40.0 23.0 41.0 56-57 34.163082629176 38.0 33.5 40.0 22.5 41.0 58-59 33.87184931365437 38.0 33.0 40.0 21.5 41.0 60-61 33.490962570568826 38.0 32.5 40.0 20.0 41.0 62-63 33.291040501163366 37.0 32.0 40.0 19.0 41.0 64-65 33.008451812682424 37.0 32.0 39.5 18.5 41.0 66-67 32.5289106058745 36.0 31.0 39.0 16.5 41.0 68-69 32.12642253079518 36.0 31.0 39.0 13.0 40.5 70-71 31.613872800612807 35.0 30.5 38.5 12.0 40.0 72-73 31.20486228120732 35.0 30.5 37.0 8.0 40.0 74-75 30.854120015915058 35.0 30.0 37.0 7.5 39.0 76-77 30.43720340795004 34.5 30.0 36.5 7.0 39.0 78-79 29.849729377524564 34.0 29.0 36.0 2.0 38.5 80-81 29.33809875244561 34.0 29.0 35.5 2.0 37.5 82-83 28.939900184450906 34.0 28.5 35.0 2.0 37.0 84-85 28.66666334228118 34.0 28.0 35.0 2.0 36.5 86-87 28.568095837484442 34.0 28.5 35.0 2.0 36.0 88-89 28.188686696264195 34.0 27.5 35.0 2.0 36.0 90-91 27.76798833735584 34.0 26.0 35.0 2.0 35.5 92-93 27.506453507069217 34.0 26.0 35.0 2.0 35.0 94-95 27.26713204564136 33.0 25.0 35.0 2.0 35.0 96-97 26.973442009244593 33.0 25.0 35.0 2.0 35.0 98-99 26.74337213766035 33.0 25.0 35.0 2.0 35.0 100-101 26.49025675103957 33.0 25.0 35.0 2.0 35.0 102-103 26.052024008362054 32.5 23.5 35.0 2.0 35.0 104-105 25.67974934833296 32.0 20.0 35.0 2.0 35.0 106-107 25.231927503201035 32.0 19.0 35.0 2.0 35.0 108-109 24.971758645414397 32.0 18.5 34.0 2.0 35.0 110-111 24.64986067325455 32.0 17.0 34.0 2.0 35.0 112-113 24.0419086031947 31.0 7.5 34.0 2.0 35.0 114-115 23.510506807816576 31.0 2.0 34.0 2.0 35.0 116-117 22.857673434065713 31.0 2.0 34.0 2.0 35.0 118-119 22.133798117417996 31.0 2.0 34.0 2.0 35.0 120-121 20.49003156766472 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.49828011726985366 1101 2 0.3095576823803583 1101 3 0.4280348293738889 1101 4 0.7138377311137347 1101 5 0.6398695212003744 1101 6 0.7512720378934219 1101 7 0.5668918569796233 1101 8 0.5705524490515472 1101 9 0.668202942197901 1101 10-11 0.8153156212408348 1101 12-13 0.8998962555614867 1101 14-15 0.9141237919732177 1101 16-17 0.7292237335021596 1101 18-19 0.6645581331725552 1101 20-21 0.7938613751088255 1101 22-23 0.819846263110577 1101 24-25 0.7479284705614404 1101 26-27 0.7786977354047409 1101 28-29 0.8808453580762432 1101 30-31 0.8277579058226863 1101 32-33 0.7827581835774566 1101 34-35 0.7592371707784622 1101 36-37 0.6439470857901171 1101 38-39 0.65433246419488 1101 40-41 0.6418066343386641 1101 42-43 0.6858305782562937 1101 44-45 0.6709603596416827 1101 46-47 0.6297067840229857 1101 48-49 0.7333196558134745 1101 50-51 0.421654076319264 1101 52-53 0.486771563201998 1101 54-55 0.6788820452526423 1101 56-57 0.7113766916615774 1101 58-59 0.790446201886013 1101 60-61 0.7335718139918654 1101 62-63 0.5955801343567373 1101 64-65 0.6830089766066081 1101 66-67 0.5840824196979852 1101 68-69 0.6353300139350146 1101 70-71 0.4118842631705597 1101 72-73 0.3853222602969488 1101 74-75 0.39323688104197885 1101 76-77 0.20940901546039825 1101 78-79 0.5717958870450204 1101 80-81 0.6373979109063832 1101 82-83 0.6767872208027335 1101 84-85 0.6227261528860275 1101 86-87 0.5174951484550405 1101 88-89 0.5947168483551373 1101 90-91 0.6386496347406059 1101 92-93 0.7076443793521143 1101 94-95 0.6442210498522662 1101 96-97 0.5802046278581479 1101 98-99 0.5347802277227629 1101 100-101 0.6206535653966299 1101 102-103 0.4515397165901476 1101 104-105 0.3895225630563992 1101 106-107 0.3707292194786369 1101 108-109 0.3549311542045537 1101 110-111 0.2269230762394514 1101 112-113 0.3040971819260605 1101 114-115 0.5146867714190115 1101 116-117 0.4184934954557846 1101 118-119 0.27622079529182386 1101 120-121 0.28200754189910526 1102 1 0.37265889152199705 1102 2 0.4644872399655604 1102 3 0.5458073308746556 1102 4 0.7796886969208998 1102 5 0.749486484367246 1102 6 0.801684468186501 1102 7 0.6421319689958764 1102 8 0.7144020307549113 1102 9 0.5412488659504575 1102 10-11 0.743847699324057 1102 12-13 0.7317564901886939 1102 14-15 0.8463327872364772 1102 16-17 0.8146506818885015 1102 18-19 0.7559480636189093 1102 20-21 0.7639982990550109 1102 22-23 0.59132569717179 1102 24-25 0.8881128236605136 1102 26-27 0.8937172023968643 1102 28-29 0.963978478603984 1102 30-31 0.961781938416614 1102 32-33 0.6869045404248268 1102 34-35 0.8493220827678911 1102 36-37 0.8590558270363644 1102 38-39 0.8102394954686645 1102 40-41 0.660978456843452 1102 42-43 0.7116866341073873 1102 44-45 0.7377568650662809 1102 46-47 0.7782650974453063 1102 48-49 0.7011564885887722 1102 50-51 0.3750024563047205 1102 52-53 0.4892577453206002 1102 54-55 0.4973976314170656 1102 56-57 0.688194761405768 1102 58-59 0.613193802309624 1102 60-61 0.6909370061189648 1102 62-63 0.5649232937390636 1102 64-65 0.6226165315726817 1102 66-67 0.49028512783071676 1102 68-69 0.40290736032838126 1102 70-71 0.2779231724544182 1102 72-73 0.38803550954016686 1102 74-75 0.2949229911765947 1102 76-77 0.29629791797370686 1102 78-79 0.5433274565649207 1102 80-81 0.5562544065125792 1102 82-83 0.642291499041999 1102 84-85 0.5043107356104883 1102 86-87 0.6133487474998418 1102 88-89 0.6874188545117086 1102 90-91 0.7137313555283207 1102 92-93 0.7192590744942073 1102 94-95 0.550805952358516 1102 96-97 0.7083258855841876 1102 98-99 0.8608554995042041 1102 100-101 0.840062423396315 1102 102-103 0.6648373019950391 1102 104-105 0.5668271421993047 1102 106-107 0.4990204687933115 1102 108-109 0.744671495802077 1102 110-111 0.6311232887509917 1102 112-113 0.6594560851583253 1102 114-115 0.5816568411667262 1102 116-117 0.452136215832855 1102 118-119 0.30289981509227104 1102 120-121 0.33329052849341423 1103 1 0.6149935746457018 1103 2 0.7378651647091061 1103 3 0.6579212854524741 1103 4 0.804110827904509 1103 5 0.9468928181450238 1103 6 0.8072315187487789 1103 7 0.7186286775198951 1103 8 0.8390893645068331 1103 9 0.7911421621013588 1103 10-11 0.8911299060419751 1103 12-13 0.9106357235131881 1103 14-15 1.0112221701865423 1103 16-17 1.0205886748217807 1103 18-19 0.8789493458083868 1103 20-21 0.9888161180312451 1103 22-23 0.9275295504809407 1103 24-25 0.9080664192291508 1103 26-27 0.9228775460069798 1103 28-29 0.9860906540579393 1103 30-31 1.0804793776307733 1103 32-33 1.046397870744201 1103 34-35 0.9437754970828394 1103 36-37 0.8479339301281215 1103 38-39 0.7089466719235631 1103 40-41 0.8628163295575462 1103 42-43 0.8862399912133796 1103 44-45 0.8364894257543085 1103 46-47 0.9784850521643236 1103 48-49 1.11194073936214 1103 50-51 0.8859117687348501 1103 52-53 0.9159056890572828 1103 54-55 0.8864164760867723 1103 56-57 0.8124244816455573 1103 58-59 0.8643113719857638 1103 60-61 0.8127723665590949 1103 62-63 0.8694768048356352 1103 64-65 0.9227383584588011 1103 66-67 0.9366300645962795 1103 68-69 0.8034462498742307 1103 70-71 0.7847441802946697 1103 72-73 0.6650282904812066 1103 74-75 0.6894669984395243 1103 76-77 0.7356325062818314 1103 78-79 0.8660595070501991 1103 80-81 0.9195889301252897 1103 82-83 0.9875637994200908 1103 84-85 1.0841591120316458 1103 86-87 0.7882705682263342 1103 88-89 0.9731539420979693 1103 90-91 0.976084087510042 1103 92-93 0.9581259672655342 1103 94-95 1.0296192215426672 1103 96-97 1.0727767924676321 1103 98-99 1.19007447575353 1103 100-101 0.9971664688256645 1103 102-103 0.9543419627281331 1103 104-105 1.0870155007633642 1103 106-107 1.0074284028140603 1103 108-109 1.147386882376054 1103 110-111 1.2304416915839553 1103 112-113 1.1837677109530844 1103 114-115 1.1290293309144914 1103 116-117 1.0125980500427225 1103 118-119 0.8703688985781177 1103 120-121 0.9746721842640369 1104 1 0.37984795338380906 1104 2 0.5183816822516363 1104 3 0.5650776645176911 1104 4 0.7457112350733581 1104 5 0.9406001142026241 1104 6 0.7751082616390619 1104 7 0.6585555816504609 1104 8 0.7228950506971117 1104 9 0.7267455989289857 1104 10-11 0.8135714830689338 1104 12-13 0.8636494629679348 1104 14-15 0.9955370668262944 1104 16-17 0.961328274839488 1104 18-19 0.8035427328891771 1104 20-21 0.8740225901704619 1104 22-23 0.768430224516635 1104 24-25 0.8538304770183629 1104 26-27 0.8572619909599055 1104 28-29 0.9257447891431525 1104 30-31 1.016415021177636 1104 32-33 0.9304340857448139 1104 34-35 0.7057205471611567 1104 36-37 0.8331679285607123 1104 38-39 0.8516823490547978 1104 40-41 0.8836095398632153 1104 42-43 0.8801174431992749 1104 44-45 0.8917074404404062 1104 46-47 1.0081491794291182 1104 48-49 0.8955672396698162 1104 50-51 0.6140035305277749 1104 52-53 0.7317863236604438 1104 54-55 0.7678895972691677 1104 56-57 0.8337807392168486 1104 58-59 0.8266845707488812 1104 60-61 0.8244240210566289 1104 62-63 0.9676383830347248 1104 64-65 0.9527337572346752 1104 66-67 0.7754312482788492 1104 68-69 0.7534256545124087 1104 70-71 0.7737400015768081 1104 72-73 0.5753122653985088 1104 74-75 0.7390784921893179 1104 76-77 0.9098663577828283 1104 78-79 0.8567132445680237 1104 80-81 0.8546929657978701 1104 82-83 0.9722433752844886 1104 84-85 0.8110961515325492 1104 86-87 0.8757100368784698 1104 88-89 1.049241906114485 1104 90-91 1.1627558437873127 1104 92-93 1.1124978679597284 1104 94-95 1.0003389972257182 1104 96-97 0.8795875185985764 1104 98-99 0.974532428067473 1104 100-101 1.0744125842307675 1104 102-103 1.10737698336402 1104 104-105 1.0278733007007261 1104 106-107 1.0239051079219408 1104 108-109 1.0018926009661016 1104 110-111 1.0802432092397467 1104 112-113 0.8395914703527012 1104 114-115 0.727507736854264 1104 116-117 0.5192026978609938 1104 118-119 0.47347887095206787 1104 120-121 0.5121679524471325 1105 1 0.719830983649171 1105 2 0.8552497491568403 1105 3 0.7749283977147599 1105 4 1.0160844515208183 1105 5 1.0431681285129457 1105 6 0.9427062861058033 1105 7 0.9339820498220845 1105 8 0.9625073271041416 1105 9 1.0225178560496602 1105 10-11 1.0429059813058146 1105 12-13 1.0835699726626729 1105 14-15 1.2314634641337463 1105 16-17 1.2113327479937368 1105 18-19 1.0094450834611024 1105 20-21 1.1229630411868285 1105 22-23 1.1370712237533738 1105 24-25 1.1319808606972899 1105 26-27 1.387559017819079 1105 28-29 1.254852051144276 1105 30-31 1.2033778012382967 1105 32-33 1.0557722208915195 1105 34-35 1.0463544213694718 1105 36-37 1.188877274298335 1105 38-39 0.9627677199098912 1105 40-41 1.0177782700665574 1105 42-43 1.0598343019526055 1105 44-45 0.9286405093396937 1105 46-47 1.1798932293099682 1105 48-49 1.1314932838335068 1105 50-51 0.9652740946772767 1105 52-53 0.9594352667482582 1105 54-55 0.9922744326170729 1105 56-57 1.142798947779788 1105 58-59 1.135561168665518 1105 60-61 1.1668798816185202 1105 62-63 1.0915819534876121 1105 64-65 1.2185573049545226 1105 66-67 1.0829621330546289 1105 68-69 0.9865185440335047 1105 70-71 0.8013569027544669 1105 72-73 0.8124193399980086 1105 74-75 0.7877581818560024 1105 76-77 0.9509410966565675 1105 78-79 1.0454370348270032 1105 80-81 1.1771231033685545 1105 82-83 1.1090698653981086 1105 84-85 0.9171232089363031 1105 86-87 1.093430578685748 1105 88-89 1.1186624348789813 1105 90-91 1.2024282488045905 1105 92-93 1.1636171924098484 1105 94-95 1.035561320054672 1105 96-97 0.8430781044923172 1105 98-99 0.999044724768499 1105 100-101 1.0921146213052104 1105 102-103 1.2654097560463278 1105 104-105 1.4891673324299894 1105 106-107 1.3271711932668957 1105 108-109 1.421070002025818 1105 110-111 1.408159199636124 1105 112-113 1.4132891585795022 1105 114-115 1.397752809204004 1105 116-117 1.4585526119999805 1105 118-119 1.5138740435240052 1105 120-121 1.6949858842395358 1106 1 0.5069546410628512 1106 2 0.6517095452882806 1106 3 0.5357470598970622 1106 4 0.8575953741894438 1106 5 0.8206594347435043 1106 6 0.827544192800211 1106 7 0.7466089363501283 1106 8 0.7767534198890402 1106 9 0.8002881067543228 1106 10-11 0.9760882861310236 1106 12-13 0.9636690112340531 1106 14-15 1.1420268257068855 1106 16-17 1.0601637049858113 1106 18-19 0.9878849337343354 1106 20-21 0.9721534380583989 1106 22-23 0.8315502484572335 1106 24-25 1.080488640591497 1106 26-27 1.021135829829099 1106 28-29 1.0632519990746374 1106 30-31 0.8941339798583883 1106 32-33 1.0582568191716248 1106 34-35 1.0291572288791855 1106 36-37 1.039954796952614 1106 38-39 1.059704378261344 1106 40-41 0.8730755584606129 1106 42-43 0.8905525972565087 1106 44-45 0.9812419986375218 1106 46-47 1.09709294558359 1106 48-49 1.1073412086194523 1106 50-51 0.9028631908706117 1106 52-53 0.8962067899710533 1106 54-55 0.9915331044694469 1106 56-57 1.0145839798849678 1106 58-59 1.3071202557466677 1106 60-61 1.2690169947823122 1106 62-63 1.262769778756791 1106 64-65 1.1381835724914922 1106 66-67 1.0697454782098959 1106 68-69 1.008754005049017 1106 70-71 0.8729551748926987 1106 72-73 0.9765745765827738 1106 74-75 0.9446936712972906 1106 76-77 1.1397276616280472 1106 78-79 1.109606749958278 1106 80-81 1.067111786192747 1106 82-83 1.1126337131316184 1106 84-85 1.2705097276827004 1106 86-87 1.033329999278223 1106 88-89 1.084107324749045 1106 90-91 1.0710861805243006 1106 92-93 1.0518353748076557 1106 94-95 1.0962575123297675 1106 96-97 1.2590771640509466 1106 98-99 1.3159492912223705 1106 100-101 1.3082388073850844 1106 102-103 1.2466353318010022 1106 104-105 1.4236735608616051 1106 106-107 1.2365074648153573 1106 108-109 1.3316412006103704 1106 110-111 1.0643085969832278 1106 112-113 1.0217749151433324 1106 114-115 0.8778838599745526 1106 116-117 0.8283578024924871 1106 118-119 0.8462513942953507 1106 120-121 0.9009556944775916 1107 1 0.4998819283033882 1107 2 0.6668983507560107 1107 3 0.6521832057827694 1107 4 0.8290232963208553 1107 5 0.7371876185010962 1107 6 0.7422123025298006 1107 7 0.7086242816767907 1107 8 0.7877192778155191 1107 9 0.787316164101874 1107 10-11 0.7644017489423192 1107 12-13 0.8503040611873232 1107 14-15 0.8661870511428518 1107 16-17 0.7247805405306096 1107 18-19 0.683090868472668 1107 20-21 0.7452917306422506 1107 22-23 0.6875561456986503 1107 24-25 0.8870192105161365 1107 26-27 0.9013975412221882 1107 28-29 0.9191044998629181 1107 30-31 0.9202916259614113 1107 32-33 0.9351590062073498 1107 34-35 0.9757073710549662 1107 36-37 0.872489190425874 1107 38-39 0.868224983097889 1107 40-41 0.9467508806641831 1107 42-43 0.9478159193123616 1107 44-45 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36098.0 3 5207.0 4 4732.0 5 5508.0 6 6627.0 7 6831.0 8 8388.0 9 10314.0 10 12758.0 11 15596.0 12 17049.0 13 20009.0 14 24619.0 15 25305.0 16 20794.0 17 20807.0 18 22097.0 19 24339.0 20 26760.0 21 29902.0 22 33313.0 23 37252.0 24 41887.0 25 47740.0 26 54670.0 27 63240.0 28 73267.0 29 86155.0 30 101784.0 31 121448.0 32 147440.0 33 183392.0 34 231845.0 35 300127.0 36 398109.0 37 436943.0 38 154666.0 39 653.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.613245277024525 18.555751423675396 14.562290982241135 21.26871231705894 2 13.592119229586705 21.216652352818873 38.35249906368727 26.838729353907155 3 22.821789290463276 24.196265848856996 31.282525919250475 21.699418941429254 4 10.891671954617035 15.32875052144249 43.46850487350211 30.311072650438366 5 10.907310730303587 38.742398663851965 36.876535135476644 13.473755470367804 6 26.94143323227521 36.74806028754444 18.541324938060864 17.769181542119494 7 24.11068909310279 33.53036394671493 23.905412601513408 18.453534358668882 8 22.351715287140912 29.924483681297914 21.58885967571138 26.13494135584979 9 21.21258421066746 20.069344564715713 26.850799798444864 31.86727142617196 10-11 24.081945386090943 23.756166571664018 28.695792696986715 23.466095345258328 12-13 27.760328334557432 22.667952924787595 27.7566586881261 21.815060052528874 14-15 22.9800416326388 24.515551725969097 30.701045438290347 21.803361203101755 16-17 19.542631686314703 28.682921561028913 30.36800451195435 21.406442240702038 18-19 18.32750053526888 31.413443447805854 29.912254736092585 20.34680128083268 20-21 20.54833036575361 27.219895539311135 27.35033019913613 24.881443895799123 22-23 19.04156049494258 26.83228279206341 29.509274879475978 24.616881833518033 24-25 22.866324261260456 26.24539556011657 28.320913667678543 22.567366510944435 26-27 21.45859830353198 24.32521706970369 32.973844353505655 21.242340273258677 28-29 19.07990749798934 29.370059048305176 30.57271578963717 20.97731766406832 30-31 19.662460856240074 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24.373653990859186 56-57 21.998322369873293 25.319323650169974 28.674283696084057 24.008070283872676 58-59 24.096500183331212 25.50153929369554 28.270167183589486 22.131793339383762 60-61 22.04370074423576 25.94960990873653 31.986159435911212 20.0205299111165 62-63 20.304420241353114 27.536982436519835 30.663473948492886 21.495123373634165 64-65 19.061303104627793 30.295937441150762 29.162713968557142 21.480045485664302 66-67 20.256879969656318 27.196303266693832 27.251847457683958 25.294969305965893 68-69 19.058501624064398 27.772648637198138 29.115096737748907 24.05375300098856 70-71 22.556814279840484 26.551145871823152 27.492626285291394 23.399413563044973 72-73 21.162545622556227 24.74022971540776 31.72476876008869 22.372455901947323 74-75 19.281592559458705 29.428772350588083 29.38145503910829 21.908180050844923 76-77 19.94894064553108 26.733852320228817 28.7057717942094 24.611435240030705 78-79 20.793110471961786 26.504399053889404 30.07554434129373 22.626946132855082 80-81 22.78399147297561 26.397763352455055 28.141495310984766 22.676749863584572 82-83 20.982041601926415 25.374857536118522 31.80163021781694 21.84147064413812 84-85 20.993510427406324 26.93037548338955 29.822846480899344 22.253267608304775 86-87 20.648524418133725 28.337824053003086 29.524383883971716 21.48926764489147 88-89 20.00414525627676 27.14168337656899 27.65973448468356 25.194436882470683 90-91 20.172338022369722 25.097618367843843 28.831544112025544 25.89849949776089 92-93 22.052092794980503 26.563971106832867 29.704068364841994 21.679867733344633 94-95 20.81457062135323 25.606291278260578 30.228679710002677 23.350458390383512 96-97 17.37590906330287 26.841078291377652 32.764625873399915 23.018386771919566 98-99 19.359752866994352 28.406538174080985 28.573552868972317 23.660156089952345 100-101 18.235461770386756 26.10836794711464 30.61810718976593 25.038063092732678 102-103 20.700357959243913 24.705967093736735 29.82687102380298 24.766803923216365 104-105 22.437660562985705 25.458261523331753 28.982248903012675 23.121829010669863 106-107 21.46017269508341 25.463804255838973 31.538000932780818 21.538022116296794 108-109 19.694631774670146 26.451388769977864 30.549903236335474 23.30407621901652 110-111 19.29973740196705 28.161044121977103 28.78204010215833 23.757178373897517 112-113 20.750956985904416 25.440209985207613 27.014745959809684 26.794087069078294 114-115 19.245807457007356 25.442143339236406 29.347403722589426 25.964645481166816 116-117 22.279668858735192 24.937893353864844 27.894625311681065 24.8878124757189 118-119 20.84305709377755 24.576151170806682 30.826857497231202 23.75393423818457 120-121 19.50241357065454 28.63217578689536 28.661782677303254 23.203627965146847 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6749.0 1 7409.0 2 4679.5 3 1357.5 4 1383.5 5 1347.5 6 1381.0 7 1425.0 8 1546.5 9 1658.5 10 1582.5 11 1555.5 12 1635.5 13 1776.5 14 2021.5 15 2195.5 16 2340.5 17 2583.5 18 2858.0 19 3142.5 20 3476.5 21 3970.5 22 4625.5 23 5564.5 24 6896.5 25 8631.0 26 11155.0 27 14869.5 28 20170.5 29 26555.0 30 33665.0 31 40988.0 32 48089.5 33 54204.5 34 59411.0 35 65021.5 36 70260.5 37 75893.0 38 80657.5 39 82628.5 40 84935.5 41 87309.5 42 90071.0 43 95388.5 44 98110.5 45 101647.0 46 117488.0 47 181930.0 48 317024.0 49 308181.5 50 168041.0 51 100611.0 52 72322.5 53 57294.5 54 47373.5 55 39037.0 56 31295.5 57 24112.0 58 18491.0 59 14552.5 60 11536.5 61 8739.5 62 6292.5 63 4296.0 64 2783.0 65 1796.0 66 1150.5 67 692.5 68 379.0 69 227.5 70 139.5 71 94.5 72 75.0 73 60.0 74 39.0 75 26.0 76 26.0 77 20.5 78 12.5 79 9.0 80 8.5 81 7.5 82 9.0 83 6.0 84 4.5 85 6.5 86 5.5 87 2.5 88 1.5 89 2.5 90 2.0 91 2.5 92 4.0 93 2.5 94 1.5 95 2.0 96 2.5 97 1.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42758596073515814 2 0.8660899032813785 3 0.8656699809040299 4 0.8463185580145509 5 0.8556618309105563 6 0.8625205630739158 7 0.8374651945587859 8 0.8298366047036204 9 0.8295916499835005 10-11 0.8237302334663438 12-13 0.8262147741989893 14-15 0.8257248647587494 16-17 0.8506577559138193 18-19 0.8742258993425065 20-21 0.8690643534542639 22-23 0.8472283898321396 24-25 0.8395997999769743 26-27 0.8405096317945627 28-29 0.8626430404339757 30-31 0.8693792952372754 32-33 0.8648301361493327 34-35 0.8742608928739521 36-37 0.8829217919067661 38-39 0.8711814621067296 40-41 0.8800523223282177 42-43 0.8732810739934723 44-45 0.8580938813460331 46-47 0.8530023225206821 48-49 0.8575164880771788 50-51 0.893629812529154 52-53 0.8619956601022302 54-55 0.8745408411255179 56-57 0.9201899028964495 58-59 0.8876284218862144 60-61 0.8995262225777565 62-63 0.8739634478566637 64-65 0.8640077881603585 66-67 0.8675421348363755 68-69 0.8843215331645945 70-71 0.8917226650653627 72-73 0.8958868953074024 74-75 0.9002610867381164 76-77 0.9120889003667673 78-79 0.9569506076801703 80-81 0.9825833694641545 82-83 0.949339514590728 84-85 0.9089394825366531 86-87 0.8935248319348169 88-89 0.8929649354316855 90-91 0.9046702717002761 92-93 0.8928074645401798 94-95 0.8877508992462744 96-97 0.8970591786108338 98-99 0.9267336932767979 100-101 0.9164106015003127 102-103 0.8925100195228911 104-105 0.8837441398957403 106-107 0.884636474947606 108-109 0.8837441398957403 110-111 0.8820119600891775 112-113 0.8913377362194599 114-115 0.8933673610433112 116-117 0.9186151939813925 118-119 0.9139960478305585 120-121 0.8944346637524053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 2857671.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.1037964408139 #Duplication Level Percentage of deduplicated Percentage of total 1 81.36523913803508 39.13976900854183 2 11.537249672642265 11.099710194792607 3 3.0171747518862335 4.354126802732957 4 1.2248254142556645 2.3567500961156016 5 0.6450667779669247 1.551508048902632 6 0.3935344166577902 1.135829968281647 7 0.2723057968895645 0.9169259836260459 8 0.20567969617490595 0.79151793894449 9 0.14990442788040365 0.6489874875902045 >10 1.0199728400614667 9.510173100018614 >50 0.10517085828159721 3.448462082240859 >100 0.0557506937148169 4.813198436828418 >500 0.0031024695749072156 1.0630192236674647 >1k 0.003176337898119003 3.3047546422637493 >5k 8.864198785448382E-4 3.1912962794756092 >10k+ 9.60288201756908E-4 12.673970705977238 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 73840 2.583922361951393 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 58418 2.044252119995619 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 44908 1.5714895101640463 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 42354 1.482116030851697 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 27396 0.9586827874867331 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 20019 0.7005355060117138 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 17862 0.6250544586833124 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 13811 0.483295662796732 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 12247 0.428565779615638 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 11901 0.41645801773542157 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 11372 0.3979464396006398 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 11290 0.3950769700220914 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 11259 0.39399217054727437 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 9360 0.32753945433186676 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 9274 0.3245300106275355 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9229 0.3229553017124785 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8920 0.31214230049575337 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 8361 0.2925809164176002 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7909 0.27676384020413825 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7406 0.259162093886945 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7221 0.2526882905694882 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 5926 0.20737166734729087 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5784 0.2024025858819997 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5371 0.18795025739492055 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 5050 0.17671733380084692 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 4850 0.16971862751170447 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4413 0.1544264542699282 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 4256 0.1489324698329514 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3894 0.13626481144960353 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3889 0.13608984379237496 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3750 0.13122574292142097 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3729 0.13049087876106102 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3375 0.11810316862927887 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3187 0.11152438471748498 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3054 0.10687024503520524 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.499353144571226E-5 0.0 10-11 0.0 3.499353144571226E-5 0.0 3.499353144571226E-5 0.0 12-13 0.0 3.499353144571226E-5 0.0 6.998706289142452E-5 0.0 14-15 0.0 1.0498059433713678E-4 0.0 1.0498059433713678E-4 0.0 16-17 0.0 1.749676572285613E-4 0.0 1.9246442295141743E-4 0.0 18-19 0.0 1.749676572285613E-4 0.0 2.449547201199858E-4 0.0 20-21 0.0 1.749676572285613E-4 0.0 3.3243854873426647E-4 0.0 22-23 0.0 1.749676572285613E-4 0.0 6.648770974685329E-4 0.0 24-25 0.0 1.749676572285613E-4 0.0 0.0012947606634913537 6.998706289142452E-5 26-27 0.0 1.749676572285613E-4 0.0 0.0021695989496341604 6.998706289142452E-5 28-29 0.0 1.749676572285613E-4 0.0 0.005021571762459709 6.998706289142452E-5 30-31 0.0 1.749676572285613E-4 0.0 0.01076051091955652 6.998706289142452E-5 32-33 0.0 1.749676572285613E-4 0.0 0.021223576821824484 6.998706289142452E-5 34-35 0.0 1.749676572285613E-4 0.0 0.034171183456738016 6.998706289142452E-5 36-37 0.0 1.749676572285613E-4 0.0 0.04573654559954593 6.998706289142452E-5 38-39 0.0 1.749676572285613E-4 0.0 0.05436245110091399 6.998706289142452E-5 40-41 0.0 1.749676572285613E-4 0.0 0.06844734750781317 6.998706289142452E-5 42-43 0.0 1.749676572285613E-4 0.0 0.08800873158596634 6.998706289142452E-5 44-45 0.0 1.749676572285613E-4 0.0 0.11880303925819312 6.998706289142452E-5 46-47 0.0 1.749676572285613E-4 0.0 0.19228945529418887 6.998706289142452E-5 48-49 0.0 2.0996118867427357E-4 0.0 0.3875008704640947 6.998706289142452E-5 50-51 0.0 2.449547201199858E-4 0.0 0.48431047520865766 6.998706289142452E-5 52-53 0.0 2.449547201199858E-4 0.0 0.5739639027725725 6.998706289142452E-5 54-55 0.0 2.449547201199858E-4 0.0 0.712538287297593 6.998706289142452E-5 56-57 0.0 2.449547201199858E-4 0.0 0.7960328533270624 6.998706289142452E-5 58-59 0.0 2.449547201199858E-4 0.0 0.9156582405742298 6.998706289142452E-5 60-61 0.0 2.449547201199858E-4 0.0 1.1382520941004055 6.998706289142452E-5 62-63 0.0 2.449547201199858E-4 0.0 1.2397333352929711 6.998706289142452E-5 64-65 0.0 2.449547201199858E-4 0.0 1.352482493611056 6.998706289142452E-5 66-67 0.0 2.449547201199858E-4 0.0 1.7223991145236803 6.998706289142452E-5 68-69 0.0 2.449547201199858E-4 0.0 2.0757113047653144 6.998706289142452E-5 70-71 0.0 2.449547201199858E-4 0.0 2.2890843627555446 6.998706289142452E-5 72-73 0.0 2.449547201199858E-4 0.0 2.508476308154438 6.998706289142452E-5 74-75 0.0 2.449547201199858E-4 0.0 2.6476630794797584 6.998706289142452E-5 76-77 0.0 2.449547201199858E-4 0.0 2.7249987839747822 6.998706289142452E-5 78-79 0.0 2.449547201199858E-4 0.0 2.8625233625564315 6.998706289142452E-5 80-81 0.0 2.449547201199858E-4 0.0 3.1470907602729636 6.998706289142452E-5 82-83 0.0 2.449547201199858E-4 0.0 3.4942615857458748 6.998706289142452E-5 84-85 0.0 2.449547201199858E-4 0.0 3.9757550816731526 6.998706289142452E-5 86-87 0.0 2.449547201199858E-4 0.0 5.039663418217142 6.998706289142452E-5 88-89 0.0 2.449547201199858E-4 0.0 6.259782879134792 6.998706289142452E-5 90-91 0.0 2.449547201199858E-4 0.0 7.390896292820273 6.998706289142452E-5 92-93 0.0 2.449547201199858E-4 0.0 8.475713264403076 6.998706289142452E-5 94-95 0.0 2.6245148584284195E-4 0.0 9.374714584009146 6.998706289142452E-5 96-97 0.0 2.799482515656981E-4 0.0 10.296251737866257 6.998706289142452E-5 98-99 0.0 2.799482515656981E-4 0.0 11.32588391035917 6.998706289142452E-5 100-101 0.0 2.799482515656981E-4 0.0 12.145572390943535 6.998706289142452E-5 102-103 0.0 2.974450172885542E-4 0.0 12.728582121594822 8.748382861428065E-5 104-105 0.0 3.1494178301141033E-4 0.0 13.306990202861002 1.0498059433713678E-4 106-107 0.0 3.1494178301141033E-4 0.0 14.255209924445467 1.0498059433713678E-4 108-109 0.0 3.1494178301141033E-4 0.0 15.285524470801573 1.0498059433713678E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2575 0.0 45.56752 2 ACCTGGG 2865 0.0 40.309357 3 GTACCCG 355 0.0 35.71619 1 GTACCTG 4115 0.0 29.831875 1 GTACCGG 210 1.3371027E-7 24.699831 1 TACCCGG 480 0.0 23.96576 2 GTACACG 395 0.0 23.344988 1 TATAACG 410 0.0 22.44598 2 CCTGGGG 2410 0.0 22.170204 4 TACCGCC 365 7.2759576E-12 22.028925 8 AGTACGC 1085 0.0 21.20473 2 TACACGG 380 1.2732926E-11 21.190779 2 GAGTACT 6810 0.0 20.968872 12-13 GTACGCA 1095 0.0 20.463737 3 TCACGCA 370 2.1827873E-10 20.185774 4 GAGTACG 1295 0.0 20.026892 1 TAACGCA 550 0.0 19.84699 4 ATAACGC 610 0.0 19.779892 3 GTACTTT 7265 0.0 19.577034 14-15 GTACATG 8955 0.0 19.500593 1 >>END_MODULE