##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003851_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2857671 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.6371324760618 31.0 31.0 34.0 30.0 34.0 2 31.83338039963313 31.0 31.0 34.0 30.0 34.0 3 31.894284191567188 31.0 31.0 34.0 30.0 34.0 4 35.55999308527819 37.0 35.0 37.0 33.0 37.0 5 35.466307703021094 37.0 35.0 37.0 33.0 37.0 6 35.55316374768124 37.0 35.0 37.0 33.0 37.0 7 35.50022658311611 37.0 35.0 37.0 33.0 37.0 8 35.51681736630984 37.0 35.0 37.0 33.0 37.0 9 37.06078656360372 39.0 37.0 39.0 33.0 39.0 10-11 36.97162059593285 39.0 37.0 39.0 33.0 39.0 12-13 36.93692188498956 39.0 37.0 39.0 33.0 39.0 14-15 37.90812658280117 40.0 37.0 41.0 33.0 41.0 16-17 38.00945227774646 40.0 37.0 41.0 33.0 41.0 18-19 38.07494897068277 40.0 37.0 41.0 33.0 41.0 20-21 38.00547596276829 40.0 37.0 41.0 33.0 41.0 22-23 37.90385299777336 40.0 37.0 41.0 33.0 41.0 24-25 37.742615227575186 39.5 37.0 41.0 32.0 41.0 26-27 37.452751733842 39.0 36.5 41.0 31.5 41.0 28-29 37.34891945223926 39.0 36.0 41.0 32.0 41.0 30-31 37.220821256190796 39.0 36.0 40.0 31.0 41.0 32-33 36.9890944408926 39.0 36.0 40.0 31.0 41.0 34-35 36.934503132096026 39.0 35.5 40.0 30.5 41.0 36-37 36.734885506414145 38.5 35.5 40.0 30.5 41.0 38-39 36.51017139481767 38.0 35.0 40.0 30.0 41.0 40-41 36.80262318510424 39.0 35.0 40.0 30.0 41.0 42-43 36.713698672800334 39.0 35.0 41.0 30.0 41.0 44-45 36.580990428919215 39.0 35.0 41.0 30.0 41.0 46-47 36.32852259759784 39.0 35.0 40.0 30.0 41.0 48-49 36.05046137221535 39.0 35.0 40.0 29.0 41.0 50-51 35.76025424200337 38.5 35.0 40.0 28.0 41.0 52-53 35.67324510064314 38.0 35.0 40.0 28.0 41.0 54-55 35.50417297862491 38.0 34.0 40.0 28.0 41.0 56-57 35.19527860275028 38.0 34.0 40.0 27.0 41.0 58-59 34.92240954259605 38.0 34.0 40.0 26.0 41.0 60-61 34.32433562156035 37.5 33.0 40.0 25.5 41.0 62-63 34.1991131939261 37.0 33.0 40.0 26.0 41.0 64-65 33.768198998415144 36.5 32.5 39.5 24.5 41.0 66-67 33.379085975957345 36.0 32.0 39.0 23.5 40.5 68-69 32.99869141689159 36.0 32.0 39.0 23.0 40.0 70-71 32.4438915466476 35.0 31.0 38.0 22.5 40.0 72-73 32.00871793848907 35.0 31.0 37.0 21.5 39.5 74-75 31.670052640769352 35.0 31.0 37.0 20.5 39.0 76-77 30.730446227014937 34.0 29.5 35.5 20.0 38.5 78-79 31.123571082885327 34.0 31.0 36.0 20.0 38.5 80-81 30.851472405325875 34.0 30.5 35.5 20.0 37.0 82-83 30.515647707521264 34.0 30.5 35.0 19.0 37.0 84-85 30.191460108598925 34.0 30.0 35.0 18.0 36.5 86-87 29.99160925102995 34.0 30.0 35.0 15.5 36.0 88-89 29.670261552152084 34.0 29.5 35.0 9.5 36.0 90-91 29.311755971908592 34.0 29.0 35.0 7.0 35.5 92-93 29.025351063855844 34.0 29.0 35.0 2.0 35.0 94-95 28.636696806595303 34.0 29.0 35.0 2.0 35.0 96-97 28.192969029674863 34.0 29.0 35.0 2.0 35.0 98-99 27.906858242253918 34.0 29.0 35.0 2.0 35.0 100-101 26.857820231930127 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11674754888656125 1101 2 0.07109417201264989 1101 3 0.07911623704097792 1101 4 0.11701922536060039 1101 5 0.15750113824261547 1101 6 0.15761439976702007 1101 7 0.1107462499019789 1101 8 0.0837079662722573 1101 9 0.12395424657604082 1101 10-11 0.19247631839176904 1101 12-13 0.1314035041486079 1101 14-15 0.1525785381826168 1101 16-17 0.15235605677968778 1101 18-19 0.05547247007840639 1101 20-21 0.04268346481129015 1101 22-23 0.07584361997042066 1101 24-25 0.14734831613448307 1101 26-27 0.14742221105078812 1101 28-29 0.09945172484915332 1101 30-31 0.22538051644112045 1101 32-33 0.33805644616700903 1101 34-35 0.3766641788737033 1101 36-37 0.173465136262692 1101 38-39 0.1027868096487552 1101 40-41 0.2522511107510823 1101 42-43 0.09339329390853379 1101 44-45 0.18472809261027834 1101 46-47 0.34977748376439877 1101 48-49 0.37636067932022854 1101 50-51 0.4182487979749112 1101 52-53 0.44288011293894414 1101 54-55 0.4288055886607083 1101 56-57 0.49073443080549595 1101 58-59 0.34642622251698896 1101 60-61 0.35914654504087906 1101 62-63 0.21426728752164337 1101 64-65 0.06545955374382828 1101 66-67 0.0672130877024344 1101 68-69 0.04346953112346341 1101 70-71 0.17379726729245704 1101 72-73 0.11221899885690689 1101 74-75 -0.016717014037816114 1101 76-77 -0.019527038386396356 1101 78-79 0.10500569526027093 1101 80-81 0.06545578297591703 1101 82-83 0.12382544499357451 1101 84-85 -0.03913567686321784 1101 86-87 -5.144316705418817E-4 1101 88-89 0.07052886271569392 1101 90-91 0.1062871315400109 1101 92-93 0.2422256577565527 1101 94-95 0.09129748696389228 1101 96-97 0.2434333844879184 1101 98-99 0.019891682243422082 1101 100-101 0.14883102300161966 1102 1 -0.03419624847146707 1102 2 -0.027340749746670667 1102 3 -0.02918128440756007 1102 4 -0.06203731691168457 1102 5 -0.11988711345961178 1102 6 0.016241649321642626 1102 7 -0.05786882147993566 1102 8 -0.12817344893638705 1102 9 -0.0678978016373506 1102 10-11 0.006900814823666224 1102 12-13 0.02580538208370342 1102 14-15 0.07482767598475704 1102 16-17 0.005083707230404855 1102 18-19 -0.13695747896381505 1102 20-21 -0.13311284697991255 1102 22-23 -0.11880018942904513 1102 24-25 -0.23678980136966743 1102 26-27 -0.3283011904923754 1102 28-29 -0.13377157414329588 1102 30-31 -0.16300474430895662 1102 32-33 -0.1256121081678856 1102 34-35 -0.19651547982870454 1102 36-37 -0.23916597978458043 1102 38-39 -0.2666443889683734 1102 40-41 -0.14469530396575436 1102 42-43 -0.10552774027900824 1102 44-45 -0.0902939943871317 1102 46-47 -0.12131261045433916 1102 48-49 -0.2307375942550749 1102 50-51 -0.19536892208238044 1102 52-53 -0.20742487067278859 1102 54-55 -0.21659469212975324 1102 56-57 -0.12257735732165287 1102 58-59 -0.2679543425131783 1102 60-61 -0.1850886714469837 1102 62-63 -0.3580712348041999 1102 64-65 -0.31957659924155024 1102 66-67 -0.20530667370947242 1102 68-69 -0.23630019070166242 1102 70-71 -0.1943106963326926 1102 72-73 -0.3214232363339953 1102 74-75 -0.2306299037139823 1102 76-77 -0.21543555168336326 1102 78-79 -0.311325979765563 1102 80-81 -0.2860099864786143 1102 82-83 -0.3847620413495392 1102 84-85 -0.34894622930941566 1102 86-87 -0.442704059184301 1102 88-89 -0.2829578835420854 1102 90-91 -0.2107324051424868 1102 92-93 -0.28517487797718744 1102 94-95 -0.29488679291619135 1102 96-97 -0.31643528002834387 1102 98-99 -0.419150498518416 1102 100-101 -0.2878878217613021 1103 1 -0.0807109913260895 1103 2 -0.09276049949357912 1103 3 -0.07381511466558521 1103 4 -0.06380840226536577 1103 5 -0.08332326116931199 1103 6 0.0031561304116465294 1103 7 -0.030854806303437954 1103 8 -0.02633725171726553 1103 9 -0.22933551966355736 1103 10-11 -0.03491585948926712 1103 12-13 -0.06734986259812104 1103 14-15 0.030563084142592345 1103 16-17 -0.06489295302774423 1103 18-19 -0.07173443039005178 1103 20-21 -0.07837437770244549 1103 22-23 -0.14192245591468833 1103 24-25 -0.1746679196667813 1103 26-27 -0.08379659610113066 1103 28-29 -0.09391677854254254 1103 30-31 -0.014002637156103503 1103 32-33 -0.06578981910664794 1103 34-35 -0.06551128789524086 1103 36-37 -0.04462169165832819 1103 38-39 -0.09987830637150807 1103 40-41 -0.055141453411970076 1103 42-43 0.012458243091757026 1103 44-45 0.05006778856034089 1103 46-47 0.17669136172779076 1103 48-49 0.26227840032987615 1103 50-51 0.13600926357350573 1103 52-53 0.19496766835776924 1103 54-55 0.13794115847498034 1103 56-57 0.11239928792483056 1103 58-59 0.06739190917696902 1103 60-61 0.140357971447024 1103 62-63 0.018264984545020013 1103 64-65 -0.03781069145788507 1103 66-67 -0.012443691524055112 1103 68-69 0.10341033800222732 1103 70-71 0.011250988177025079 1103 72-73 0.18711738139291256 1103 74-75 0.20420631955763824 1103 76-77 0.10394768796268039 1103 78-79 0.08238948070452423 1103 80-81 0.11765650982992071 1103 82-83 0.010436910119892673 1103 84-85 -0.12059335805822613 1103 86-87 -0.14009973952581234 1103 88-89 -0.11189870825558046 1103 90-91 -0.10558671794887786 1103 92-93 -0.11648589855991531 1103 94-95 -0.22820722590670428 1103 96-97 -0.17271766465864857 1103 98-99 -0.17171243202799857 1103 100-101 -0.3033024723862141 1104 1 -0.07883122293350553 1104 2 -0.05820507770742722 1104 3 -0.10353769244009214 1104 4 -0.03863872952795333 1104 5 0.013862427319502046 1104 6 -0.04419480935824538 1104 7 -0.07142288914542405 1104 8 -0.1282790971489689 1104 9 -0.16307737270162193 1104 10-11 -0.10291183102817314 1104 12-13 -0.07368428721545683 1104 14-15 -0.031117946282215314 1104 16-17 -0.1046890215373466 1104 18-19 -0.1853129150327888 1104 20-21 -0.09387415397986842 1104 22-23 -0.21969109289867816 1104 24-25 -0.15525310227032918 1104 26-27 -0.07713547210146743 1104 28-29 -0.06292647415722286 1104 30-31 -0.03514010797501754 1104 32-33 0.03271409723337371 1104 34-35 0.06729159537228924 1104 36-37 0.08296189880529425 1104 38-39 -0.0706713400267418 1104 40-41 -0.02686101856927081 1104 42-43 -0.05195936226412101 1104 44-45 -0.009449689752699442 1104 46-47 0.04523860730077445 1104 48-49 0.05238267188273937 1104 50-51 0.13362967837780815 1104 52-53 0.21956852671259952 1104 54-55 0.07830106398154868 1104 56-57 -0.06100861890681841 1104 58-59 0.048000939588483504 1104 60-61 -0.008686256209244902 1104 62-63 0.21957830774900344 1104 64-65 0.12806613996544058 1104 66-67 0.10427054606412156 1104 68-69 0.08587383423012085 1104 70-71 0.11900203493473072 1104 72-73 0.13892823533564425 1104 74-75 0.060056867475314135 1104 76-77 0.10138719194490164 1104 78-79 0.15322999176045116 1104 80-81 0.1512770351966104 1104 82-83 0.13200976189999736 1104 84-85 0.21262265860759655 1104 86-87 0.10689050084969764 1104 88-89 0.11488183974730148 1104 90-91 0.08296688496132631 1104 92-93 -0.047207860551811365 1104 94-95 0.01619950193920161 1104 96-97 0.06786816635588977 1104 98-99 -0.026299513727575885 1104 100-101 0.11803618248934455 1105 1 0.09957041075659845 1105 2 0.15016103913795575 1105 3 0.19064635949370157 1105 4 0.1795341894312088 1105 5 0.06438435024846711 1105 6 0.11283856649359336 1105 7 0.11761873049426441 1105 8 0.1281171602805884 1105 9 0.13415784128701347 1105 10-11 0.18804872469767275 1105 12-13 0.035519886849570526 1105 14-15 0.131421764733183 1105 16-17 0.22162404934896784 1105 18-19 0.20210379601227402 1105 20-21 0.163872172030203 1105 22-23 0.19217011124619177 1105 24-25 0.14729080409288997 1105 26-27 0.22929513444412208 1105 28-29 0.24048239897987855 1105 30-31 0.05371415713047867 1105 32-33 0.018380816165311842 1105 34-35 0.24480224736512923 1105 36-37 0.25164301517275334 1105 38-39 0.23026058280333928 1105 40-41 0.22982104987375607 1105 42-43 0.3134044629137165 1105 44-45 0.3180152183522367 1105 46-47 0.20707838739109974 1105 48-49 0.19815394020088917 1105 50-51 0.22608648065638448 1105 52-53 0.09290433676081733 1105 54-55 0.17117175074256608 1105 56-57 0.24010481981402876 1105 58-59 0.027781693818788256 1105 60-61 0.13631009699289365 1105 62-63 0.13909743425028154 1105 64-65 0.13012496806494767 1105 66-67 -0.03280196753308928 1105 68-69 0.10621032372691985 1105 70-71 0.17037644689151676 1105 72-73 0.16128953341675256 1105 74-75 0.012865325597879007 1105 76-77 -0.085448809507227 1105 78-79 0.06375419364379198 1105 80-81 0.2324042794840402 1105 82-83 0.26508033273569254 1105 84-85 0.14281908658204756 1105 86-87 0.28560172389953564 1105 88-89 0.31144612768471447 1105 90-91 0.39573289049579685 1105 92-93 0.2688105989786216 1105 94-95 0.364958743122731 1105 96-97 0.4039826244850424 1105 98-99 0.3487724687414264 1105 100-101 0.3184259590246832 1106 1 -0.030696392991352184 1106 2 -0.03050252991674185 1106 3 0.07867193737366662 1106 4 0.05688230416505036 1106 5 0.05068605127843284 1106 6 -0.025345212752405644 1106 7 0.0385966832543545 1106 8 0.04620896754855153 1106 9 -0.016885543876860254 1106 10-11 -0.1566171928038287 1106 12-13 -0.1352361058075502 1106 14-15 -0.02618345710737202 1106 16-17 -0.052051694967879314 1106 18-19 -0.029600235930857366 1106 20-21 -0.10368871092204301 1106 22-23 -0.09094524690522832 1106 24-25 0.0762894731292647 1106 26-27 0.087129090753443 1106 28-29 0.20647614531043246 1106 30-31 0.11144835758262417 1106 32-33 0.21468745849739435 1106 34-35 0.11918584649269093 1106 36-37 0.10891005004612708 1106 38-39 0.21232830520843038 1106 40-41 -0.12442315671517434 1106 42-43 0.0783348994751023 1106 44-45 0.16738839026969998 1106 46-47 0.13222284077947677 1106 48-49 0.1394589752814781 1106 50-51 0.22252010913953768 1106 52-53 0.18303209332995607 1106 54-55 0.15889434179136686 1106 56-57 0.13915428331082325 1106 58-59 0.10098349819104868 1106 60-61 0.43272433219356543 1106 62-63 0.16871589134197507 1106 64-65 0.1526712529230494 1106 66-67 0.030145572309486113 1106 68-69 0.17993574672628654 1106 70-71 0.1713661684517973 1106 72-73 0.3083874482403921 1106 74-75 0.20065499615117233 1106 76-77 0.053979991908221336 1106 78-79 0.1326815060270654 1106 80-81 0.12048272398338611 1106 82-83 0.267617531973773 1106 84-85 0.2602739706341275 1106 86-87 0.25051728342230106 1106 88-89 0.31427475748288813 1106 90-91 0.3055138792344074 1106 92-93 0.1719710398702894 1106 94-95 0.24266616490537274 1106 96-97 0.46179625769697097 1106 98-99 0.33197748761667256 1106 100-101 0.4099307714810152 1107 1 -0.06247814960799403 1107 2 -0.014783086348170826 1107 3 -0.03129542326991697 1107 4 -0.0957997191909783 1107 5 -0.08431617865148411 1107 6 -0.0029408085697966158 1107 7 -0.16087012203725237 1107 8 -0.11158541870940297 1107 9 -0.04357482231698384 1107 10-11 -0.08502351728278512 1107 12-13 0.01740202911883415 1107 14-15 -0.058259365626277315 1107 16-17 -0.10728862147109197 1107 18-19 -0.10964747635706829 1107 20-21 -0.06569468202101092 1107 22-23 -0.12790455991636662 1107 24-25 0.0017011579620458406 1107 26-27 -0.07622605896433043 1107 28-29 -0.16264585884263738 1107 30-31 -0.03664526538593549 1107 32-33 -0.07924869448999772 1107 34-35 -0.17948159283758258 1107 36-37 -0.06343612029778711 1107 38-39 -0.00576580067489374 1107 40-41 0.06341293065262477 1107 42-43 0.1479212119663984 1107 44-45 0.07296378320607033 1107 46-47 -0.12090318008812062 1107 48-49 -0.13720246454307983 1107 50-51 0.23987720187905381 1107 52-53 0.149952290245551 1107 54-55 -0.019964644258074316 1107 56-57 -0.20583590215685632 1107 58-59 0.07883816357372808 1107 60-61 0.14806610801316822 1107 62-63 -0.12444305294954461 1107 64-65 -0.035533696101040846 1107 66-67 0.007929646916970512 1107 68-69 -0.056849148836299435 1107 70-71 0.0889381582594595 1107 72-73 0.027104656197689536 1107 74-75 0.06736313667368421 1107 76-77 -0.05222546275602724 1107 78-79 -0.03411100254467314 1107 80-81 0.07752646185725709 1107 82-83 0.14473688861768963 1107 84-85 -0.08200038744432803 1107 86-87 0.08730331525490342 1107 88-89 -0.009684348158973677 1107 90-91 0.10465585333313143 1107 92-93 0.12478387631274046 1107 94-95 0.14355359179349847 1107 96-97 0.4504068551193434 1107 98-99 0.46496709926798374 1107 100-101 0.5254293525638261 1108 1 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-0.944023606238467 2316 68-69 -0.9712721384925906 2316 70-71 -1.0843557117353342 2316 72-73 -0.8598954104752607 2316 74-75 -0.8541428261498254 2316 76-77 -0.9169384063257446 2316 78-79 -0.6593420448837612 2316 80-81 -0.838669088442785 2316 82-83 -0.8036686732576008 2316 84-85 -0.9128005157383612 2316 86-87 -0.8162278252725876 2316 88-89 -0.9150558971361313 2316 90-91 -1.0742324500869564 2316 92-93 -1.1441201818965645 2316 94-95 -1.3342878581640463 2316 96-97 -1.2907091942938322 2316 98-99 -1.5543250278312222 2316 100-101 -1.844578436716752 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 8.0 4 22.0 5 36.0 6 127.0 7 323.0 8 798.0 9 1441.0 10 2537.0 11 4071.0 12 6116.0 13 8502.0 14 10686.0 15 13944.0 16 17297.0 17 21856.0 18 20407.0 19 15853.0 20 15437.0 21 15776.0 22 17156.0 23 19172.0 24 22337.0 25 27375.0 26 34390.0 27 42808.0 28 54439.0 29 68546.0 30 84564.0 31 103986.0 32 128738.0 33 162920.0 34 212315.0 35 282354.0 36 400032.0 37 514994.0 38 438578.0 39 87701.0 40 28.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.521972780184676 20.21397750645486 14.240217058334428 19.023832655026037 2 14.382466717573386 25.67910428435346 37.04884735472862 22.889581643344528 3 26.27049428542322 25.65224803927369 28.38110855122779 19.696149124075298 4 12.535522808608828 16.86240298480826 41.53476729826491 29.067306908317995 5 12.002781285879305 41.27819472570495 33.67088093765867 13.04814305075707 6 27.596013635022732 40.894115111030395 16.34271064839845 15.167160605548421 7 27.59926793506598 34.26063891212071 21.211931398656947 16.92816175415636 8 24.450540317622288 31.40316012585074 18.990534599679247 25.155764956847726 9 22.877371117948847 23.46309284728718 22.617999062873228 31.04153697189075 10-11 25.208054842073587 25.915696758886643 27.698309106051806 21.17793929298797 12-13 30.43025246783132 23.820289319519286 25.543388304671883 20.20606990797751 14-15 22.78459041474693 27.372744521797678 28.964605938274033 20.87805912518136 16-17 19.734409594386477 29.42329610371523 29.30909471384215 21.53319958805615 18-19 19.53323178210508 33.51711936048621 27.912590357672386 19.03705849973632 20-21 22.866635775226978 27.404422608894073 26.878474849491724 22.85046676638722 22-23 20.77413740070148 28.616187797685598 27.118114716494656 23.491560085118266 24-25 24.1619486637895 28.80775988558515 27.275603104766084 19.754688345859268 26-27 23.824071282500935 25.620062184260195 31.191920980599836 19.363945552639034 28-29 20.18111254851229 32.29982769267597 28.526127561315036 18.992932197496707 30-31 22.185561006714334 28.33332954200059 26.987925538458203 22.49318391282688 32-33 22.172083466243127 29.185258727471027 27.76146892692933 20.881188879356518 34-35 24.549584703132908 26.860804332777832 28.944427840813248 19.645183123276013 36-37 23.7959834760931 26.075537110101337 30.40876631587053 19.71971309793504 38-39 21.27776145538717 28.82863933849346 30.88058840400209 19.01301080211727 40-41 21.311448444526025 29.5176517917248 28.154025529648248 21.01687423410093 42-43 19.915413635789424 30.99524052978807 26.237554987960475 22.85179084646203 44-45 22.833331758624418 26.452660225757267 26.995287421120207 23.71872059449811 46-47 24.739770512340474 29.679284172070254 26.367477700364283 19.213467615224992 48-49 24.057475475658325 28.82027007307699 27.44889107248525 19.67336337877943 50-51 19.927661371795423 29.315096104485082 30.648769574944073 20.108472948775418 52-53 22.289724744381 31.859178330885534 25.8968404690393 19.954256455694164 54-55 20.282460787123497 29.1648338804572 27.0834711203634 23.469234212055902 56-57 23.192154210064302 27.392743893594513 27.69589672857043 21.719205167770756 58-59 26.747134461177218 26.860671212335944 25.80286702605099 20.589327300435844 60-61 22.049730360801554 28.856127509007656 30.13698150733028 18.95716062286051 62-63 20.975294258052525 28.247163114734303 29.46279859348308 21.314744033730086 64-65 20.41462963355197 32.62847859380079 26.92817010898933 20.028721663657905 66-67 22.356285931798492 27.513646426727572 26.483675915960063 23.646391725513872 68-69 21.090730939154017 29.494772757097238 26.2964349765977 23.118061327151043 70-71 24.04524677074357 29.150590736309834 26.060477750609106 20.743684742337486 72-73 23.530420401788728 26.200076915782116 29.4512909288718 20.818211753557357 74-75 20.60571713918732 32.604861197258394 26.868353086893677 19.921068576660605 76-77 22.482119180269528 28.302593265634847 26.006702661013108 23.208584893082516 78-79 22.124677753317297 29.473704285762775 26.764050165326942 21.637567795592986 80-81 23.983979955698175 28.2823069143743 27.10671631084065 20.62699681908688 82-83 23.152910184552386 27.19685016224751 28.860775085725404 20.789464567474703 84-85 20.79698159636725 29.241100134217763 28.800916271108328 21.16100199830666 86-87 21.70348541999764 29.242485163492905 26.469836897279475 22.58419251922998 88-89 20.77602705139955 29.95567019436457 25.78904289542078 23.479259858815098 90-91 22.780386545547053 25.51985515477464 27.022284930630576 24.677473369047732 92-93 23.589996889075053 29.02914646227645 26.677143729981513 20.703712918666984 94-95 22.60931732073751 28.51061722726966 27.616793699195885 21.26327175279695 96-97 18.725555843475515 29.067158584038033 30.511416415135244 21.69586915735121 98-99 21.399704092937775 31.21788012124631 25.79213578690772 21.590279998908198 100-101 19.812172991635197 29.224992253194475 26.18159561972607 24.781239135444256 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 189.0 1 138.5 2 163.5 3 252.0 4 235.0 5 223.0 6 319.0 7 560.5 8 853.5 9 1112.5 10 1175.5 11 1218.0 12 1379.5 13 1596.0 14 1857.5 15 2130.5 16 2362.0 17 2562.0 18 2874.5 19 3203.5 20 3471.0 21 3959.0 22 4655.5 23 5488.0 24 6727.0 25 8461.0 26 10889.0 27 14385.0 28 19777.0 29 26396.5 30 33082.0 31 40122.5 32 47548.5 33 54365.0 34 59804.0 35 64501.5 36 69327.0 37 74332.5 38 79522.0 39 82168.5 40 83469.0 41 85581.0 42 88928.0 43 94196.5 44 96407.0 45 99160.5 46 111427.5 47 183680.0 48 335867.0 49 322665.0 50 172218.0 51 104268.0 52 74338.5 53 57682.0 54 47032.5 55 39341.0 56 31889.0 57 24630.0 58 18989.0 59 14986.0 60 11644.0 61 8674.5 62 6328.5 63 4303.0 64 2708.5 65 1663.5 66 1019.5 67 581.5 68 324.0 69 177.5 70 79.5 71 42.0 72 29.5 73 13.5 74 9.0 75 8.0 76 6.0 77 4.0 78 3.0 79 1.0 80 1.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04535161675364309 2 0.005493984436976825 3 0.042132211860637564 4 0.0 5 0.0 6 0.0010498059433713678 7 3.4993531445712255E-5 8 0.0 9 0.0 10-11 3.849288459028349E-4 12-13 0.0 14-15 0.002554527795536995 16-17 0.0 18-19 0.0 20-21 0.012352716600336428 22-23 0.0 24-25 0.0 26-27 0.0068412353976367475 28-29 0.00967571144473944 30-31 0.00967571144473944 32-33 0.005476487671253969 34-35 0.006893725694805315 36-37 0.020943628570258787 38-39 0.011005465639676505 40-41 0.011425388017025053 42-43 0.0 44-45 0.0 46-47 3.4993531445712255E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0012772638977684974 58-59 2.0996118867427357E-4 60-61 0.00845093784413951 62-63 0.013997412578284905 64-65 0.04515915233069167 66-67 0.024355497886215733 68-69 0.0016097024465027638 70-71 2.9744501728855424E-4 72-73 0.0 74-75 1.7496765722856127E-5 76-77 0.0 78-79 0.0 80-81 3.4993531445712255E-5 82-83 0.0 84-85 5.249029716856839E-5 86-87 8.398447546970943E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.3997412578284902E-4 96-97 3.3243854873426647E-4 98-99 5.948900345771085E-4 100-101 0.0014522315549970587 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2857671.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.4074430221283 #Duplication Level Percentage of deduplicated Percentage of total 1 79.1269206265959 35.13824219242026 2 12.717795207142071 11.29529532056516 3 3.4865810435881586 4.644904471055213 4 1.403059154541493 2.4922507784790744 5 0.7338807243303589 1.629488322536933 6 0.486995434903606 1.2975733216511085 7 0.30795886539755774 0.9572966037810927 8 0.23208474288526504 0.8245031996786164 9 0.17313992448717244 0.6919831198367736 >10 1.130830990148504 9.768498182291257 >50 0.12239143537869426 3.7878644421408403 >100 0.06949544533303252 5.701097208176104 >500 0.0034524055911115776 0.9830467480951097 >1k 0.003217014300808018 3.160990250608912 >5k 9.415651612121029E-4 2.7201819562003164 >10k+ 0.0012554202149494707 14.90678388248331 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 127146 4.449287549196531 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 37072 1.297280197755445 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 29719 1.0399727610351228 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 27348 0.957003097977339 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 26986 0.944335439593991 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 24151 0.8451287779453969 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 22579 0.7901189465127372 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 21656 0.7578199169883447 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 19722 0.6901424271723372 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 17738 0.620715260784044 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 14430 0.504956658761628 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 12572 0.4399386773354945 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 12360 0.4325200486690035 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 11720 0.4101241885437477 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 11529 0.4034404240376166 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 11090 0.38807826373294896 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 9594 0.33572794069016343 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7818 0.2735794288425784 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 7378 0.25818227500646507 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6578 0.23018744984989523 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 6272 0.2194794292275073 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6235 0.21818466856401594 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 6180 0.21626002433450175 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 5846 0.20457218483163386 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 5824 0.2038023271398282 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5547 0.19410911892936591 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5457 0.19095970109925178 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5339 0.18683046438865777 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4582 0.1603403610842536 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3642 0.12744644152528406 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3596 0.1258367390787813 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3537 0.12377212072348426 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 3519 0.12314223715746145 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3181 0.11131442352881071 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3173 0.111034475277245 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2931 0.10256604066738265 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2906 0.10169120238123983 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 2876 0.10064139643786846 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2870 0.10043143524919419 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.499353144571226E-5 0.0 10-11 0.0 0.0 0.0 3.499353144571226E-5 0.0 12-13 0.0 0.0 0.0 1.0498059433713678E-4 0.0 14-15 0.0 0.0 0.0 1.3997412578284905E-4 0.0 16-17 0.0 0.0 0.0 2.2745795439712971E-4 0.0 18-19 0.0 0.0 0.0 2.974450172885542E-4 3.499353144571226E-5 20-21 0.0 0.0 0.0 4.02425611625691E-4 3.499353144571226E-5 22-23 0.0 0.0 0.0 7.173673946371013E-4 3.499353144571226E-5 24-25 0.0 0.0 0.0 0.001347250960659922 3.499353144571226E-5 26-27 0.0 0.0 0.0 0.002309573075417009 3.499353144571226E-5 28-29 0.0 0.0 0.0 0.005371507076916832 3.499353144571226E-5 30-31 0.0 0.0 0.0 0.011267917125519347 3.499353144571226E-5 32-33 0.0 0.0 0.0 0.022308376296641563 3.499353144571226E-5 34-35 0.0 0.0 0.0 0.03562341501173508 3.499353144571226E-5 36-37 0.0 1.749676572285613E-5 0.0 0.047398738343217256 3.499353144571226E-5 38-39 0.0 3.499353144571226E-5 0.0 0.0562346050332596 3.499353144571226E-5 40-41 0.0 3.499353144571226E-5 0.0 0.07058195292600163 3.499353144571226E-5 42-43 0.0 3.499353144571226E-5 0.0 0.09066823997584046 3.499353144571226E-5 44-45 0.0 3.499353144571226E-5 0.0 0.12272231478011289 3.499353144571226E-5 46-47 0.0 6.998706289142452E-5 0.0 0.19836083300001994 3.499353144571226E-5 48-49 0.0 6.998706289142452E-5 0.0 0.3990662326069026 3.499353144571226E-5 50-51 0.0 6.998706289142452E-5 0.0 0.49725808184357123 3.499353144571226E-5 52-53 0.0 6.998706289142452E-5 0.0 0.588731173042663 3.499353144571226E-5 54-55 0.0 6.998706289142452E-5 0.0 0.7300700465518949 3.499353144571226E-5 56-57 0.0 6.998706289142452E-5 0.0 0.8151743150278671 3.499353144571226E-5 58-59 0.0 6.998706289142452E-5 0.0 0.9366018691444886 3.499353144571226E-5 60-61 0.0 6.998706289142452E-5 0.0 1.1633249593812582 3.499353144571226E-5 62-63 0.0 6.998706289142452E-5 0.0 1.2672557477750237 5.249029716856839E-5 64-65 0.0 6.998706289142452E-5 0.0 1.3816146085396115 6.998706289142452E-5 66-67 0.0 6.998706289142452E-5 0.0 1.7594222707932439 6.998706289142452E-5 68-69 0.0 6.998706289142452E-5 0.0 2.1220777339308827 6.998706289142452E-5 70-71 0.0 6.998706289142452E-5 0.0 2.342187046724413 6.998706289142452E-5 72-73 0.0 6.998706289142452E-5 0.0 2.5684552210523885 6.998706289142452E-5 74-75 0.0 6.998706289142452E-5 0.0 2.7140108151008286 6.998706289142452E-5 76-77 0.0 6.998706289142452E-5 0.0 2.796088143106747 6.998706289142452E-5 78-79 0.0 6.998706289142452E-5 0.0 2.9418362015781385 6.998706289142452E-5 80-81 0.0 6.998706289142452E-5 0.0 3.240191050684281 6.998706289142452E-5 82-83 0.0 6.998706289142452E-5 0.0 3.5993471606773486 6.998706289142452E-5 84-85 0.0 6.998706289142452E-5 0.0 4.0977600290586285 6.998706289142452E-5 86-87 0.0 6.998706289142452E-5 0.0 5.19279511182358 6.998706289142452E-5 88-89 0.0 8.748382861428065E-5 0.0 6.44734820768381 6.998706289142452E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2680 0.0 44.50426 2 ACCTGGG 2875 0.0 39.8315 3 TATAACG 375 0.0 35.480476 2 GTACCTG 3600 0.0 34.069237 1 AGTACGC 1400 0.0 31.565859 2 TACGCAG 1490 0.0 29.324364 4 GAGTACG 1605 0.0 29.322794 1 GTACGCA 1505 0.0 29.046835 3 GTACCCG 300 0.0 28.523083 1 ATAACGC 525 0.0 26.247393 3 CCTGGGG 2185 0.0 25.213526 4 TAACGCA 590 0.0 24.148842 4 TCACGCA 480 0.0 23.74636 4 CGCAGTG 1885 0.0 22.92752 6 GTACACG 355 0.0 22.7649 1 ACGCAGT 1870 0.0 22.603489 5 AATGCGT 360 0.0 22.427118 6 GTATAAC 775 0.0 22.082386 1 GGACCGT 115 0.0037279224 20.649008 6 TATCACG 570 0.0 20.007788 2 >>END_MODULE