##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003850_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 881206 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.894454872073045 31.0 31.0 34.0 28.0 34.0 2 30.82892195468483 31.0 31.0 34.0 27.0 34.0 3 30.910562342970884 31.0 31.0 34.0 27.0 34.0 4 34.40253811254122 37.0 35.0 37.0 30.0 37.0 5 34.36810348545062 37.0 35.0 37.0 30.0 37.0 6 34.46103181321961 37.0 35.0 37.0 30.0 37.0 7 34.48464263747637 37.0 35.0 37.0 30.0 37.0 8 34.47073102089636 37.0 35.0 37.0 30.0 37.0 9 35.93478482897302 39.0 35.0 39.0 30.0 39.0 10-11 35.77231771004737 39.0 35.0 39.0 30.0 39.0 12-13 35.64027310299748 39.0 35.0 39.0 30.0 39.0 14-15 36.60293620333951 39.0 36.0 41.0 30.0 41.0 16-17 36.67717707323827 39.0 36.0 41.0 30.0 41.0 18-19 36.66926291922661 39.0 36.0 41.0 30.0 41.0 20-21 36.577184562973926 39.0 36.0 41.0 30.0 41.0 22-23 36.4867902624358 39.0 36.0 41.0 30.0 41.0 24-25 36.249475151099745 39.0 36.0 41.0 29.0 41.0 26-27 36.086610281818324 39.0 35.5 41.0 28.0 41.0 28-29 35.937109484047994 39.0 35.0 40.0 27.0 41.0 30-31 35.65702117325574 38.5 35.0 40.0 27.0 41.0 32-33 35.39526285567733 38.0 34.5 40.0 27.0 41.0 34-35 35.359506744166524 38.0 35.0 40.0 26.5 41.0 36-37 35.18420097003425 38.0 34.5 40.0 25.0 41.0 38-39 35.155064763517274 38.0 34.5 40.0 25.5 41.0 40-41 35.32235595309156 38.5 35.0 40.0 25.5 41.0 42-43 35.17817797427617 38.0 35.0 40.0 25.0 41.0 44-45 34.94989650547091 38.0 34.0 40.0 24.0 41.0 46-47 34.662021706615704 38.0 34.0 40.0 23.5 41.0 48-49 34.37290769695168 38.0 34.0 40.0 23.0 41.0 50-51 33.536869925987794 37.5 32.5 39.5 20.5 40.5 52-53 33.65659221566807 38.0 33.0 39.5 21.0 40.5 54-55 33.99616888673023 38.0 33.0 40.0 21.0 41.0 56-57 33.71369464120762 38.0 33.0 40.0 18.5 41.0 58-59 33.41160806894189 38.0 32.5 40.0 16.0 41.0 60-61 33.040417337149314 37.5 32.0 40.0 13.0 41.0 62-63 32.829977893931726 37.0 32.0 40.0 13.0 41.0 64-65 32.53083444733694 36.5 31.0 39.5 9.5 41.0 66-67 32.04952077039874 36.0 31.0 39.0 8.0 41.0 68-69 31.676208514240713 36.0 31.0 39.0 7.0 40.5 70-71 31.15582395035894 35.0 30.0 38.5 4.0 40.0 72-73 30.757860250611095 35.0 30.0 37.5 2.0 40.0 74-75 30.395665712671043 35.0 29.5 37.0 2.0 39.5 76-77 29.957907118199376 34.5 29.0 36.5 2.0 39.0 78-79 29.373864907864903 34.0 29.0 36.0 2.0 39.0 80-81 28.855415192361377 34.0 28.0 36.0 2.0 38.0 82-83 28.428869640016067 34.0 26.5 35.0 2.0 37.0 84-85 28.121887504170424 34.0 26.0 35.0 2.0 37.0 86-87 27.994400855191635 34.0 26.0 35.0 2.0 36.0 88-89 27.59647744114316 34.0 26.0 35.0 2.0 36.0 90-91 27.189713869401707 33.5 25.0 35.0 2.0 36.0 92-93 26.930061756274924 33.0 25.0 35.0 2.0 35.0 94-95 26.71738787525278 33.0 25.0 35.0 2.0 35.0 96-97 26.414106349707104 33.0 25.0 35.0 2.0 35.0 98-99 26.140135791177094 33.0 24.0 35.0 2.0 35.0 100-101 25.857251312405953 33.0 21.5 35.0 2.0 35.0 102-103 25.385080219608128 32.0 19.5 35.0 2.0 35.0 104-105 24.972198328200214 32.0 18.5 35.0 2.0 35.0 106-107 24.488091887708435 31.5 16.0 34.5 2.0 35.0 108-109 24.180515112243903 31.0 8.5 34.0 2.0 35.0 110-111 23.814879267730817 31.0 4.5 34.0 2.0 35.0 112-113 23.175346627235854 31.0 2.0 34.0 2.0 35.0 114-115 22.60874131587847 31.0 2.0 34.0 2.0 35.0 116-117 21.93263606920516 30.0 2.0 34.0 2.0 35.0 118-119 21.21314539392605 29.5 2.0 34.0 2.0 35.0 120-121 19.632941105711943 27.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.589772309326765 1101 2 0.43933071767907705 1101 3 0.5154576254525054 1101 4 0.8099682476267347 1101 5 0.7080084613367461 1101 6 0.6980954145387059 1101 7 0.5497423709941884 1101 8 0.6953147209180841 1101 9 0.8002845237915537 1101 10-11 0.9170185187016457 1101 12-13 1.0869817283242256 1101 14-15 1.1792927654606515 1101 16-17 0.9791884480254609 1101 18-19 0.6113099291322612 1101 20-21 0.861182271889831 1101 22-23 0.8139556498716161 1101 24-25 0.8814028615631315 1101 26-27 0.7939611336071195 1101 28-29 0.8429938454484684 1101 30-31 0.7306477568449807 1101 32-33 0.7657237932970276 1101 34-35 0.834296807768176 1101 36-37 0.9465188777064668 1101 38-39 0.9221870437753381 1101 40-41 0.8041148779091785 1101 42-43 0.9103831132529834 1101 44-45 0.9975518401556371 1101 46-47 0.995045451293656 1101 48-49 1.2376211567342565 1101 50-51 0.79886317976203 1101 52-53 1.0256961976561456 1101 54-55 1.2149055811429506 1101 56-57 1.36328784168186 1101 58-59 1.080712642262661 1101 60-61 1.0055298249610871 1101 62-63 1.021844507704266 1101 64-65 0.8340118362300188 1101 66-67 1.0010080457528403 1101 68-69 1.0625290107939627 1101 70-71 0.860767752292201 1101 72-73 0.771027901055664 1101 74-75 0.6735790743947447 1101 76-77 0.6727010687057273 1101 78-79 0.9622415020220991 1101 80-81 1.0908359071841396 1101 82-83 0.8194828830795906 1101 84-85 0.9357395938228557 1101 86-87 0.8656717837315 1101 88-89 0.9088367169089544 1101 90-91 1.134001788451382 1101 92-93 1.0631674803939148 1101 94-95 1.0837015436023236 1101 96-97 1.0268019489003848 1101 98-99 1.2745027950454464 1101 100-101 0.9684663246810175 1101 102-103 0.6697492791084301 1101 104-105 0.6237893091990792 1101 106-107 0.7814494321245569 1101 108-109 0.5439707872902098 1101 110-111 0.5531740682544388 1101 112-113 0.7248125255489057 1101 114-115 0.7082092520090022 1101 116-117 0.43495241776427207 1101 118-119 0.5383842698369747 1101 120-121 0.596554203329692 1102 1 0.487113703006127 1102 2 0.7012102218031018 1102 3 0.6907108130795834 1102 4 0.8057570316843012 1102 5 0.787735165229229 1102 6 0.7676068166102255 1102 7 0.69134155527167 1102 8 0.786192216010555 1102 9 0.7782850832686989 1102 10-11 0.8744519354721589 1102 12-13 0.8856871200127259 1102 14-15 1.022318555903766 1102 16-17 0.9307022454341833 1102 18-19 0.7946407193876723 1102 20-21 0.7583719891358953 1102 22-23 0.7993320435691373 1102 24-25 1.0065892025705026 1102 26-27 0.7906242520745863 1102 28-29 0.8980714638196545 1102 30-31 0.895376234837066 1102 32-33 0.7120145930465966 1102 34-35 0.8016806224092861 1102 36-37 0.9562591689009992 1102 38-39 0.6611724888926886 1102 40-41 0.9775924522003479 1102 42-43 1.0026868512142428 1102 44-45 0.8976627667926849 1102 46-47 0.9225135386931882 1102 48-49 0.9333549013196176 1102 50-51 0.7762328137162413 1102 52-53 0.8554713411281227 1102 54-55 0.8022875550800279 1102 56-57 0.9887094173971605 1102 58-59 1.0494691134877314 1102 60-61 1.2543055145893263 1102 62-63 1.1916037387743827 1102 64-65 1.0271089539539346 1102 66-67 1.1364793204767025 1102 68-69 0.7465288727411625 1102 70-71 0.7175192003328767 1102 72-73 0.7028086247672007 1102 74-75 0.747611100222727 1102 76-77 0.83609291705109 1102 78-79 1.0421213960847382 1102 80-81 1.2438798503596722 1102 82-83 1.3184450650888913 1102 84-85 1.0514148233961897 1102 86-87 1.251709196040725 1102 88-89 1.1231727737576414 1102 90-91 1.2922060605800922 1102 92-93 0.977825115500984 1102 94-95 0.645337578289908 1102 96-97 1.1068468402806815 1102 98-99 1.2463124735156264 1102 100-101 1.0049143835254206 1102 102-103 0.9275011255646497 1102 104-105 1.048905957761452 1102 106-107 1.1331286506972624 1102 108-109 1.1355733623382527 1102 110-111 0.9851785222737739 1102 112-113 0.8986506719599561 1102 114-115 1.055556458437902 1102 116-117 0.9474613336743722 1102 118-119 1.0726737399200843 1102 120-121 1.1395204130900254 1103 1 0.6176831613649938 1103 2 0.5949958255867998 1103 3 0.6059847012097173 1103 4 0.7448505526409903 1103 5 0.9240946502410452 1103 6 0.8718733098201028 1103 7 0.5899000094426157 1103 8 0.6995105322247852 1103 9 0.820305826971655 1103 10-11 0.8245451127222694 1103 12-13 0.917584903209864 1103 14-15 1.0356269025356895 1103 16-17 0.9734902286713449 1103 18-19 0.9224542505509774 1103 20-21 1.1663074280728978 1103 22-23 1.007661106714238 1103 24-25 1.1096115895116085 1103 26-27 1.1694080745797706 1103 28-29 1.1435574391271643 1103 30-31 0.9750070535349806 1103 32-33 1.0836928223372269 1103 34-35 0.9200840530610819 1103 36-37 0.8514213419353069 1103 38-39 0.7614277938608183 1103 40-41 0.9856618879010739 1103 42-43 0.9900707979557524 1103 44-45 1.0943751750184987 1103 46-47 1.1560398496758424 1103 48-49 1.000912764984534 1103 50-51 0.8816723014888552 1103 52-53 0.8799006522578736 1103 54-55 0.7838758977798861 1103 56-57 0.6546374286467511 1103 58-59 0.8124381108754264 1103 60-61 0.8442367244713438 1103 62-63 1.011821145386243 1103 64-65 1.0313732162083369 1103 66-67 0.9724626106201342 1103 68-69 0.8180149609907907 1103 70-71 0.8023174696691662 1103 72-73 0.4765451196433226 1103 74-75 0.7167618661027753 1103 76-77 0.70744870355702 1103 78-79 0.8877127840388326 1103 80-81 0.8300508544918195 1103 82-83 0.8971841987670466 1103 84-85 0.8886206074271463 1103 86-87 0.9991674754302515 1103 88-89 1.1140202788870965 1103 90-91 1.107157366490803 1103 92-93 0.930269510678496 1103 94-95 1.0628804178639868 1103 96-97 0.971077164124786 1103 98-99 0.8133973049007075 1103 100-101 0.8014540321359895 1103 102-103 0.8080941135321318 1103 104-105 1.22386341274321 1103 106-107 0.9487499404265698 1103 108-109 1.163495164559869 1103 110-111 1.2010077788845486 1103 112-113 1.1831982376737962 1103 114-115 1.285320472149909 1103 116-117 1.0816494715446332 1103 118-119 1.134609247180105 1103 120-121 1.3860845673522313 1104 1 0.6821657914061596 1104 2 0.6571874710945735 1104 3 0.7405552720801012 1104 4 0.9864764331270806 1104 5 1.0858053327033304 1104 6 0.8694575275158201 1104 7 0.9215897939509006 1104 8 0.8750094774945367 1104 9 0.945054435098335 1104 10-11 1.1493422785270724 1104 12-13 0.9881398902422447 1104 14-15 1.2443606287172742 1104 16-17 1.0512827300649192 1104 18-19 1.1447084915952317 1104 20-21 1.0799084185890209 1104 22-23 0.8857907889942425 1104 24-25 1.2345318891170152 1104 26-27 0.9189915147475602 1104 28-29 1.0201089968680677 1104 30-31 1.0899170592151748 1104 32-33 1.159546798356395 1104 34-35 1.0728429536002793 1104 36-37 0.9527938148749868 1104 38-39 0.6977597529059736 1104 40-41 1.1224228487380188 1104 42-43 1.1464439175747927 1104 44-45 1.1919530393731534 1104 46-47 1.4768026439054935 1104 48-49 1.6665420974526057 1104 50-51 1.5416653128136844 1104 52-53 1.6077893235700387 1104 54-55 1.6777985488778455 1104 56-57 1.6704848546738233 1104 58-59 1.4048981320986016 1104 60-61 1.4262534413218404 1104 62-63 1.46833592938399 1104 64-65 1.3383768671103908 1104 66-67 1.32179577453104 1104 68-69 1.1651308845056398 1104 70-71 1.269005541044411 1104 72-73 1.259209801647259 1104 74-75 1.3901608052460865 1104 76-77 1.2810612359760754 1104 78-79 1.1573482005873217 1104 80-81 1.2283527728226424 1104 82-83 1.1388270971309886 1104 84-85 1.122569155953439 1104 86-87 1.2539769167976118 1104 88-89 1.4778462457483705 1104 90-91 1.4180040323859942 1104 92-93 1.3262369543436492 1104 94-95 1.1545349262023734 1104 96-97 1.350381458151361 1104 98-99 1.3305456531454851 1104 100-101 1.4695301572635273 1104 102-103 1.3372646470388219 1104 104-105 1.240778048798873 1104 106-107 1.018190445547436 1104 108-109 1.3155101947495815 1104 110-111 1.20428920390869 1104 112-113 1.1244207566373845 1104 114-115 1.0895214676579457 1104 116-117 0.7055199571743564 1104 118-119 0.5383228506665709 1104 120-121 0.5534875408671169 1105 1 0.9091817967472906 1105 2 1.0449666322727467 1105 3 1.0385006217495878 1105 4 1.2378102115544536 1105 5 1.2445625579630146 1105 6 1.1083527319282709 1105 7 0.9880832348285793 1105 8 1.173983355286147 1105 9 1.1791799155292324 1105 10-11 1.1732796165038195 1105 12-13 1.3015041395670792 1105 14-15 1.6563853855696138 1105 16-17 1.5507880140529622 1105 18-19 1.3281428733054739 1105 20-21 1.1805883899586505 1105 22-23 1.257922488712154 1105 24-25 1.553601161388265 1105 26-27 1.6402588899558168 1105 28-29 1.6607136029899152 1105 30-31 1.6481912972923638 1105 32-33 1.539936796972988 1105 34-35 1.432871403530207 1105 36-37 1.6699170036730493 1105 38-39 1.5720718119470973 1105 40-41 1.630046587888856 1105 42-43 1.9791878020428157 1105 44-45 1.6840631460152835 1105 46-47 1.7168060723325667 1105 48-49 1.7642432468778964 1105 50-51 1.5132289161506876 1105 52-53 1.491055892797199 1105 54-55 1.6715597138212743 1105 56-57 1.6706156762332114 1105 58-59 1.706883913148424 1105 60-61 1.815739297556469 1105 62-63 1.9072242167944395 1105 64-65 1.6495246684059737 1105 66-67 1.5840659345844514 1105 68-69 1.566114001005996 1105 70-71 1.303512358802461 1105 72-73 1.5449932573814529 1105 74-75 1.539959009231481 1105 76-77 1.6422084357932256 1105 78-79 1.8572600839967421 1105 80-81 1.8508799446108988 1105 82-83 1.8782696469183655 1105 84-85 2.057480167219129 1105 86-87 1.8935725428464423 1105 88-89 1.9189885735902088 1105 90-91 1.767241959210125 1105 92-93 1.7619870280247554 1105 94-95 1.6032724229694253 1105 96-97 1.4731071178228312 1105 98-99 1.4745681604684826 1105 100-101 1.7273403547288986 1105 102-103 1.813410012346747 1105 104-105 1.7195353342064053 1105 106-107 1.80923984497122 1105 108-109 2.183563870685031 1105 110-111 2.0939735480265362 1105 112-113 1.7751843335093334 1105 114-115 2.06204593279325 1105 116-117 1.7672917923452083 1105 118-119 2.115833872758877 1105 120-121 1.932644508694036 1106 1 0.4804223121842348 1106 2 0.6541481253828145 1106 3 0.6379359086848737 1106 4 0.8305027372826927 1106 5 0.8510158764699085 1106 6 0.8781749236254583 1106 7 0.7042903907678806 1106 8 0.7149741105626148 1106 9 0.8908573749745514 1106 10-11 0.8087766839205273 1106 12-13 0.9016221515154541 1106 14-15 0.800816475313205 1106 16-17 0.6723730262629672 1106 18-19 0.6020954831955763 1106 20-21 0.638940801436064 1106 22-23 0.6613489233885872 1106 24-25 0.9403301318225914 1106 26-27 0.6348679515291664 1106 28-29 0.791646905798224 1106 30-31 1.028922916863273 1106 32-33 1.292290222317483 1106 34-35 0.8348138311422773 1106 36-37 0.8029747940969116 1106 38-39 1.0045678873003183 1106 40-41 0.9600112660678164 1106 42-43 0.6139367336667476 1106 44-45 0.6753471596207206 1106 46-47 0.875067453808235 1106 48-49 1.1246198494419986 1106 50-51 0.8243866324423337 1106 52-53 0.7249975463817151 1106 54-55 0.8161993004251471 1106 56-57 0.7273758803148453 1106 58-59 1.0079043213116918 1106 60-61 1.0047859899513725 1106 62-63 0.9075144771293466 1106 64-65 0.8681969931050872 1106 66-67 0.7042214487131773 1106 68-69 0.4699219985639971 1106 70-71 0.5067790821584701 1106 72-73 0.7230277724695391 1106 74-75 0.6992070033009021 1106 76-77 0.7416464481776686 1106 78-79 0.9842850927181779 1106 80-81 0.5445966941312186 1106 82-83 0.8155788654775833 1106 84-85 0.807879445520193 1106 86-87 0.6496720980531379 1106 88-89 0.6872640728924928 1106 90-91 0.7909179360111018 1106 92-93 0.7146446134948405 1106 94-95 0.7693531180679756 1106 96-97 0.8843392388763789 1106 98-99 0.972890646514184 1106 100-101 0.8390524273784692 1106 102-103 1.1622743176842683 1106 104-105 1.310448141637071 1106 106-107 1.1915713031955377 1106 108-109 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0.8776943540456728 2204 66-67 0.7619717256309215 2204 68-69 0.862703120114201 2204 70-71 0.8528820488482687 2204 72-73 0.8139076702435197 2204 74-75 0.9040210346893645 2204 76-77 0.974686904858391 2204 78-79 1.1029601914743843 2204 80-81 1.0368988814477227 2204 82-83 0.9620132993643047 2204 84-85 0.8623767730971927 2204 86-87 0.8382313918128119 2204 88-89 0.9407760070461286 2204 90-91 1.0505741910156416 2204 92-93 1.075156894629739 2204 94-95 1.1949915302283358 2204 96-97 1.3156452896957873 2204 98-99 1.3233980232023832 2204 100-101 1.2914355047019086 2204 102-103 1.4125758410654647 2204 104-105 1.335399931259662 2204 106-107 1.3914775515273554 2204 108-109 1.413528259864254 2204 110-111 1.568105347403698 2204 112-113 1.5681435250508606 2204 114-115 1.8093176844270502 2204 116-117 1.7189166245589185 2204 118-119 1.724637676491799 2204 120-121 1.6077779902615426 2205 1 1.1173927564079058 2205 2 1.294422749282898 2205 3 1.3814251224231668 2205 4 1.349281122181928 2205 5 1.4024413306282923 2205 6 1.3348214251866324 2205 7 1.2279607356444018 2205 8 1.2669894384082028 2205 9 1.4439149608388675 2205 10-11 1.613202510630721 2205 12-13 1.67203488569087 2205 14-15 1.9319081407971694 2205 16-17 1.8253809385536286 2205 18-19 1.808243031449912 2205 20-21 1.9687334142660333 2205 22-23 1.1468992356224845 2205 24-25 1.742360982659946 2205 26-27 1.9320489762240243 2205 28-29 2.089621820643522 2205 30-31 -3.7572013025520015 2205 32-33 -1.47202862880372 2205 34-35 1.2899368619398999 2205 36-37 2.0285659983262647 2205 38-39 2.1283260479588435 2205 40-41 2.2581059297067654 2205 42-43 2.245434214506936 2205 44-45 2.180075019399318 2205 46-47 2.2186134373449917 2205 48-49 2.2331854362997845 2205 50-51 2.3534611960245115 2205 52-53 2.36097895427271 2205 54-55 2.300827103433946 2205 56-57 2.32389340799012 2205 58-59 2.388439376662241 2205 60-61 2.212880564887506 2205 62-63 2.2581322922017293 2205 64-65 2.242902056261485 2205 66-67 2.3517630503311153 2205 68-69 2.465992464076347 2205 70-71 2.2522241928595754 2205 72-73 2.122203723363665 2205 74-75 2.1063085502779124 2205 76-77 2.2659731938489713 2205 78-79 2.2402060052926878 2205 80-81 2.329425852518959 2205 82-83 2.2300604862553293 2205 84-85 2.123087098798649 2205 86-87 2.2494006674542177 2205 88-89 2.405603822395758 2205 90-91 2.4832921554263727 2205 92-93 2.40461720432652 2205 94-95 2.182180431437267 2205 96-97 2.2960533319788503 2205 98-99 2.5205262731108355 2205 100-101 2.584300174327094 2205 102-103 2.464673285183558 2205 104-105 2.743517436272324 2205 106-107 2.5845691033658476 2205 108-109 2.6571824078908115 2205 110-111 2.7055950724050426 2205 112-113 2.44541970933248 2205 114-115 2.672953087783167 2205 116-117 2.614138526118669 2205 118-119 2.6478037332738076 2205 120-121 2.4059011545258002 2206 1 0.9763134595389893 2206 2 1.2189038864993336 2206 3 1.2634755062181426 2206 4 1.3257863302719812 2206 5 1.4974831354767204 2206 6 1.4140388882473331 2206 7 1.297822320336202 2206 8 1.3041334744055249 2206 9 1.4466560198950305 2206 10-11 1.4887349969682404 2206 12-13 1.6913632055563497 2206 14-15 1.8120815881012646 2206 16-17 1.666811723114641 2206 18-19 1.6605419088690638 2206 20-21 1.6289207186707202 2206 22-23 1.5794213836287696 2206 24-25 1.770625186188056 2206 26-27 1.916588577949156 2206 28-29 1.9197049632470993 2206 30-31 1.7875006919362946 2206 32-33 1.6910289637975282 2206 34-35 1.7597392814830783 2206 36-37 1.6035990841001606 2206 38-39 1.854324459841699 2206 40-41 1.8483794906702187 2206 42-43 1.7700457485969636 2206 44-45 1.8401942060342193 2206 46-47 1.8853198069403305 2206 48-49 1.9891377616458215 2206 50-51 2.132610930559828 2206 52-53 2.016789983902001 2206 54-55 1.9358540391463421 2206 56-57 1.9729191292599921 2206 58-59 2.1230941947294113 2206 60-61 2.0807196356832733 2206 62-63 2.098428040095918 2206 64-65 1.9483277339359901 2206 66-67 1.975226702689163 2206 68-69 2.0485632676324848 2206 70-71 2.1808165294158535 2206 72-73 2.1306845803241323 2206 74-75 2.056317113654689 2206 76-77 2.0531717240621212 2206 78-79 1.9622559219943874 2206 80-81 1.961948000525247 2206 82-83 1.8873514230887487 2206 84-85 1.6977138134202256 2206 86-87 1.8088269990593062 2206 88-89 1.7409180204744494 2206 90-91 1.7312061946933497 2206 92-93 2.0325918100219447 2206 94-95 2.0710398674999766 2206 96-97 2.1763649610445057 2206 98-99 2.1521866340182427 2206 100-101 2.128113913097394 2206 102-103 2.04902527110848 2206 104-105 2.0317673156065617 2206 106-107 1.9961051396122862 2206 108-109 2.1171957200492493 2206 110-111 1.7895402979332466 2206 112-113 1.8007562500396581 2206 114-115 1.8886999699355336 2206 116-117 1.8900536397001844 2206 118-119 1.8054819682840062 2206 120-121 1.5248515180800695 2207 1 0.9162607704687566 2207 2 1.1523828644170457 2207 3 1.2031529463003885 2207 4 1.2845774802216496 2207 5 1.2154466814939866 2207 6 1.2691375196166064 2207 7 1.2111745017922146 2207 8 1.0812372890085342 2207 9 1.3731783270765945 2207 10-11 1.606527196415648 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1.9646130082459656 2207 80-81 1.993829171679053 2207 82-83 2.022899410837468 2207 84-85 2.030836176289423 2207 86-87 2.0928542860005557 2207 88-89 1.9945120147771753 2207 90-91 2.021310930284173 2207 92-93 1.986042301572418 2207 94-95 1.9369654602520185 2207 96-97 2.0870066539486203 2207 98-99 2.282414310772939 2207 100-101 2.119754342320551 2207 102-103 2.0815736229686586 2207 104-105 2.1845634774433975 2207 106-107 2.1712545324028802 2207 108-109 2.352780118944068 2207 110-111 2.3138907873418866 2207 112-113 2.325646481143348 2207 114-115 2.3758746791818233 2207 116-117 2.5266297068680394 2207 118-119 2.631663724157981 2207 120-121 2.3378984884585314 2208 1 0.7722327705614731 2208 2 1.0994194636336019 2208 3 1.0999705065071446 2208 4 1.2835344373178188 2208 5 1.3595720320722933 2208 6 1.2220731059226182 2208 7 0.9822683061173691 2208 8 1.031166593878389 2208 9 1.3274605976651017 2208 10-11 1.3566906064705861 2208 12-13 1.4693760371845812 2208 14-15 1.5938283079440438 2208 16-17 1.5409924063121494 2208 18-19 1.516694732405277 2208 20-21 1.6520060894585455 2208 22-23 1.4629888074771031 2208 24-25 1.438476755461508 2208 26-27 1.5893704980693855 2208 28-29 1.664997142889895 2208 30-31 1.7548262800525904 2208 32-33 1.753424201067041 2208 34-35 1.8851886774717528 2208 36-37 1.7295340011569778 2208 38-39 1.6896750176129203 2208 40-41 1.7181558041781102 2208 42-43 1.7720340729712802 2208 44-45 1.821380264643551 2208 46-47 1.6627430772715215 2208 48-49 1.6555674477911353 2208 50-51 1.565244882354527 2208 52-53 1.719864950666917 2208 54-55 1.683250143417773 2208 56-57 1.6700984830545735 2208 58-59 1.7499134537317858 2208 60-61 1.8306416055116088 2208 62-63 1.76321620262987 2208 64-65 1.6461139890657535 2208 66-67 1.5500663073362944 2208 68-69 1.8303562275769956 2208 70-71 1.6064508858114372 2208 72-73 1.5785719856764224 2208 74-75 1.7320815203363829 2208 76-77 1.627057626611105 2208 78-79 1.6997739845341258 2208 80-81 1.8254241001726648 2208 82-83 1.7627227889057515 2208 84-85 1.7630279571113405 2208 86-87 1.9034458072206846 2208 88-89 2.0367564113189083 2208 90-91 2.133593339945069 2208 92-93 1.975837865163264 2208 94-95 2.2232969624655894 2208 96-97 2.1296566782862882 2208 98-99 1.8621166958643762 2208 100-101 1.7733829726893262 2208 102-103 1.7902714418500558 2208 104-105 1.9830475884231618 2208 106-107 2.1954635582008066 2208 108-109 2.4047051125699177 2208 110-111 2.4607442745819945 2208 112-113 2.525055423497843 2208 114-115 2.5514535679934482 2208 116-117 2.5567087463414175 2208 118-119 2.536902117408335 2208 120-121 2.3172219012949142 2209 1 0.5315622005217016 2209 2 0.6973398127277264 2209 3 0.7388974471464138 2209 4 0.8802856156946035 2209 5 1.0348325638148168 2209 6 0.8889146878934469 2209 7 0.7835009987083268 2209 8 0.7586310584534388 2209 9 0.9593669903591646 2209 10-11 1.1421164792058889 2209 12-13 1.0677783239720284 2209 14-15 1.2187173748169258 2209 16-17 0.9466053463622259 2209 18-19 0.9197327350566198 2209 20-21 1.086366359748716 2209 22-23 0.9887372608575689 2209 24-25 1.1067577062642826 2209 26-27 0.9414358795688713 2209 28-29 1.055410863614597 2209 30-31 1.2153373089228197 2209 32-33 1.1638201540792679 2209 34-35 1.0591663435492293 2209 36-37 1.037407951665152 2209 38-39 1.1501556664566408 2209 40-41 1.4235270849263841 2209 42-43 1.1738002572399537 2209 44-45 1.045810869298144 2209 46-47 1.0443447490935895 2209 48-49 1.1827580318241289 2209 50-51 1.0308266138319127 2209 52-53 0.8569764545703009 2209 54-55 1.0069948959586483 2209 56-57 1.0968315254761407 2209 58-59 0.7916744826020121 2209 60-61 0.7161112070729629 2209 62-63 0.7729184783700376 2209 64-65 0.618216378961165 2209 66-67 0.8762709214178983 2209 68-69 0.9897731151893048 2209 70-71 0.8409524692552459 2209 72-73 0.9499586037350483 2209 74-75 0.7884594846904811 2209 76-77 0.7545962000387121 2209 78-79 0.5018099066139605 2209 80-81 0.7619896842056626 2209 82-83 0.7495894113832868 2209 84-85 0.8408160149990991 2209 86-87 0.8567130409811767 2209 88-89 0.9075455555227663 2209 90-91 1.0495640278225196 2209 92-93 1.1199059348347902 2209 94-95 1.0618469145940317 2209 96-97 1.1545393009111216 2209 98-99 0.993187637829319 2209 100-101 1.1272619497109773 2209 102-103 1.2367353908852614 2209 104-105 1.3546091217859555 2209 106-107 1.3587161543121624 2209 108-109 1.3659949748471298 2209 110-111 1.502403116090317 2209 112-113 1.5349998188432536 2209 114-115 1.3909572688330414 2209 116-117 1.110333175565163 2209 118-119 1.3251524120025309 2209 120-121 1.4664352427295881 2210 1 0.5535432333879307 2210 2 0.6842745680681723 2210 3 0.6694748234229273 2210 4 0.7602926792731424 2210 5 1.0018831871835445 2210 6 0.9453457118906101 2210 7 0.8984401505720925 2210 8 0.8916615236993621 2210 9 1.2287601561093595 2210 10-11 1.0428660737628164 2210 12-13 1.1941185825686844 2210 14-15 1.4573804104452464 2210 16-17 1.1870085404934372 2210 18-19 1.061197524859729 2210 20-21 1.1531722126995803 2210 22-23 1.224655240990856 2210 24-25 1.1366793258826533 2210 26-27 1.0462013402535604 2210 28-29 1.1260997050610158 2210 30-31 1.2913717430763327 2210 32-33 1.1408464983764617 2210 34-35 1.1788777662743115 2210 36-37 1.127068678168456 2210 38-39 1.1791705823272025 2210 40-41 1.1665985220953772 2210 42-43 1.3150959804490299 2210 44-45 1.568979327727554 2210 46-47 1.429628907672516 2210 48-49 0.8756598480823925 2210 50-51 0.7423202122658878 2210 52-53 0.9175205084942206 2210 54-55 1.0773650053015729 2210 56-57 0.8265265087516056 2210 58-59 0.741997015734313 2210 60-61 0.706184582766646 2210 62-63 0.7273275134155242 2210 64-65 0.8757111367089294 2210 66-67 0.827757519623809 2210 68-69 0.9186028100933932 2210 70-71 0.8094965801946081 2210 72-73 0.7238029326000621 2210 74-75 0.7795460721268164 2210 76-77 0.7490288806509255 2210 78-79 0.6077980315664497 2210 80-81 0.34080322473730007 2210 82-83 0.541931288584717 2210 84-85 0.796939868355409 2210 86-87 0.700190633916332 2210 88-89 0.8476650978788989 2210 90-91 0.7349940508820936 2210 92-93 0.8586964633044971 2210 94-95 0.8200274412899233 2210 96-97 0.9331582762804516 2210 98-99 0.642560547263475 2210 100-101 0.6461890934912269 2210 102-103 0.7436968167778666 2210 104-105 1.110650381953704 2210 106-107 1.0398759590659843 2210 108-109 0.9725120763092576 2210 110-111 1.116500102507974 2210 112-113 1.1910463054256226 2210 114-115 1.3943389966540387 2210 116-117 1.1576377710089965 2210 118-119 1.187749179861033 2210 120-121 1.3781131910609332 2211 1 0.29052376084570497 2211 2 0.5570412124719368 2211 3 0.4553071387763694 2211 4 0.5253625305731049 2211 5 0.4873203071713661 2211 6 0.4519475854350148 2211 7 0.4510129000867522 2211 8 0.6255976280143329 2211 9 0.7063383559979286 2211 10-11 0.7559211900201603 2211 12-13 0.7211351344026085 2211 14-15 0.7894617438662408 2211 16-17 0.7840671553717513 2211 18-19 0.7382278323992395 2211 20-21 0.6874148356617482 2211 22-23 0.8050608007183087 2211 24-25 0.6592680977663363 2211 26-27 0.8111535326880954 2211 28-29 0.9557279376518224 2211 30-31 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-0.9160545067985844 2316 72-73 -1.142010276138297 2316 74-75 -0.7461239311021153 2316 76-77 -0.9435750596090244 2316 78-79 -1.0369882083672373 2316 80-81 -0.9686097121925172 2316 82-83 -1.0300588173916942 2316 84-85 -0.8817405108271394 2316 86-87 -0.5871878594739961 2316 88-89 -0.6260359801985871 2316 90-91 -0.619395006414571 2316 92-93 -0.28692231076188435 2316 94-95 -0.2534391778263121 2316 96-97 -0.24810825994381958 2316 98-99 -0.427456657638718 2316 100-101 -0.5603215402949573 2316 102-103 -0.2553423352180424 2316 104-105 -0.5147568489245131 2316 106-107 -0.7612218775500956 2316 108-109 -0.6877200211948242 2316 110-111 -0.6948899523838534 2316 112-113 -0.43953263712349866 2316 114-115 -0.6631968227956939 2316 116-117 -1.173217437931772 2316 118-119 -0.8704059196816196 2316 120-121 -1.0393255676800734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11572.0 3 1879.0 4 1601.0 5 1777.0 6 2155.0 7 2325.0 8 2862.0 9 3499.0 10 4728.0 11 5696.0 12 6337.0 13 7282.0 14 9149.0 15 9347.0 16 7626.0 17 7426.0 18 7840.0 19 8366.0 20 9136.0 21 10054.0 22 11260.0 23 12266.0 24 13574.0 25 15255.0 26 17217.0 27 19762.0 28 22908.0 29 26632.0 30 31509.0 31 37109.0 32 44744.0 33 54605.0 34 68868.0 35 87701.0 36 115353.0 37 129731.0 38 51725.0 39 330.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.002888503096536 18.345414358846924 14.425303013549975 21.226394124506566 2 13.450534614042414 21.06385047969382 38.90491364367073 26.58070126259304 3 22.35802116099514 24.514726908778954 32.11003717472119 21.017214755504718 4 10.732408573300898 15.320297945193728 43.73259498918674 30.214698492318636 5 10.796958976840049 38.12419150622605 37.104988957018726 13.97386055991518 6 26.01960515178551 36.19323770960584 19.70775284240386 18.07940429620479 7 23.318484122400196 33.378929369985535 24.802680863961022 18.49990564365325 8 21.690977170161325 29.90649395801835 22.321593994386895 26.08093487743343 9 20.971405436192335 19.67400230110641 27.933167269391856 31.421424993309405 10-11 23.62322644393324 23.405193339268774 29.441330911124492 23.530249305673493 12-13 27.59173016087232 22.292356220221006 28.564043561104263 21.55187005780241 14-15 23.01832519864927 24.227691093593183 31.457180681483514 21.296803026274027 16-17 19.281153604384528 28.541024029230194 31.23748447803588 20.94033788834939 18-19 17.737203727752764 31.160088559872307 30.98050881880124 20.12219889357369 20-21 19.7954262667868 27.501380129002733 28.12888831681469 24.574305287395777 22-23 18.35119191290611 26.879440574799556 30.17926452470478 24.590102987589553 24-25 22.228850371904016 25.867647353204436 29.319019580367584 22.58448269452396 26-27 21.07932349963681 24.00106382370268 33.90966649698869 21.009946179671815 28-29 18.61077089326872 28.858914123804464 31.81687387697125 20.713441105955564 30-31 19.105691522045337 26.907748621487066 30.425402397622747 23.561157458844853 32-33 20.06158130775035 26.33808046418255 31.66437157488302 21.935966653184074 34-35 22.83069832001094 25.036030824878463 30.160732665869094 21.972538189241504 36-37 21.443493396610215 24.380157959515078 33.69070581685371 20.485642827021 38-39 21.360773072896105 26.65070401583678 31.916261879015696 20.072261032251422 40-41 19.745552023939965 28.532332401635287 31.840957140936254 19.881158433488487 42-43 18.706244493013997 28.512913524585475 28.660815434436827 24.120026547963704 44-45 19.425934274148336 26.907278539669612 28.592301548980885 25.07448563720117 46-47 21.846629100998253 27.615228397357054 29.434855982380366 21.103286519264323 48-49 21.36508192585397 26.398597698032532 29.996229940451364 22.240090435662133 50-51 18.144463704949164 27.574944760376496 33.34979600969905 20.93079552497529 52-53 19.386253581465173 28.95008146975092 29.40977911628205 22.253885832501854 54-55 18.346268458587765 26.640788787065944 31.158622552205877 23.854320202140418 56-57 21.364683906582375 25.208154613280804 29.389960621021544 24.03720085911528 58-59 23.33641103441648 25.323930899317986 29.320995069957632 22.018662996307903 60-61 21.6293187653534 25.409238047880006 32.83792359363851 20.123519593128087 62-63 20.092776650542046 27.162661832713642 31.532159406009715 21.2124021107346 64-65 18.80333860473419 29.889872731142535 30.32613645986145 20.980652204261823 66-67 19.333469507091046 27.280810313960615 28.268070057393235 25.1176501215551 68-69 18.498686366307798 27.669743734130382 30.023754709023414 23.807815190538403 70-71 21.592771422274758 26.474122115337828 28.368275084361073 23.564831378026337 72-73 20.099111125358345 24.80800582508266 32.52870181255324 22.564181237005762 74-75 18.785113284170443 28.80248172992926 30.57441812271946 21.83798686318084 76-77 19.303019512156023 26.672490962989766 29.76894795117209 24.25554157368212 78-79 20.155107535806156 25.978251288174437 30.97789067946694 22.888750496552465 80-81 22.201675822854668 25.91966446902888 29.187038135389447 22.691621572727 82-83 20.280543820587525 25.282779491824197 32.48931257546464 21.947364112123637 84-85 20.665577307757246 26.571638702957006 30.368903477267978 22.393880512017766 86-87 20.126919922788186 28.16462849683616 30.12611301012189 21.582338570253764 88-89 19.672779423412372 26.267723637162753 28.471417845190295 25.588079094234583 90-91 19.352837310729097 24.74963726951948 30.04856438859524 25.848961031156186 92-93 21.193892670185534 25.74377211572084 30.642409647104202 22.419925566989424 94-95 19.972622604549432 25.307266054604856 30.996659660174647 23.72345168067107 96-97 16.750653148706153 26.70174926529713 33.72988688294259 22.817710703054132 98-99 18.92573106010083 28.39535148644928 29.362732779164695 23.316184674285196 100-101 17.500808526592653 26.559053923715158 30.699396967953724 25.240740581738464 102-103 20.359968524214896 24.71472923671221 29.99842621074469 24.926876028328206 104-105 22.008770157886875 25.406848688739508 29.678051772886903 22.906329380486714 106-107 21.20598609683688 25.594787114967758 32.04754634578453 21.15168044241083 108-109 20.4341476701787 26.339901895032718 30.21540289187624 23.01054754291234 110-111 19.89254633931964 27.339969694611412 28.838301067097593 23.92918289897136 112-113 21.34970610167377 25.05973252419489 27.1725613249146 26.41800004921674 114-115 20.18086941393136 24.61956415988206 29.213237507568657 25.986328918617918 116-117 22.644790551056705 24.696663802691372 27.489469023559966 25.169076622691954 118-119 21.5182062599563 24.468276923587688 30.64408941224687 23.369427404209137 120-121 19.675845511541993 28.53091743618065 29.428351499300355 22.364885552976997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2008.0 1 2110.5 2 1419.5 3 629.0 4 566.5 5 511.0 6 530.0 7 630.5 8 746.0 9 789.0 10 783.0 11 738.5 12 744.5 13 830.0 14 913.0 15 973.0 16 1055.5 17 1171.5 18 1272.0 19 1371.0 20 1517.0 21 1739.0 22 2030.5 23 2447.0 24 3016.0 25 3706.0 26 4654.5 27 6015.0 28 8137.0 29 10581.0 30 12939.0 31 15626.5 32 17860.0 33 19256.0 34 20459.0 35 21856.5 36 23049.5 37 24169.0 38 25118.0 39 25461.5 40 25877.5 41 25887.5 42 25873.5 43 26547.5 44 27206.5 45 28629.0 46 33749.5 47 52137.5 48 91311.0 49 89516.5 50 48426.0 51 28452.0 52 20577.5 53 16825.0 54 14604.5 55 12553.0 56 10370.0 57 8321.0 58 6512.5 59 5148.0 60 4108.5 61 3182.0 62 2357.5 63 1655.0 64 1089.5 65 698.0 66 447.5 67 260.0 68 153.0 69 88.5 70 55.0 71 40.0 72 30.5 73 21.0 74 13.5 75 11.5 76 9.5 77 6.5 78 4.0 79 0.5 80 2.5 81 3.5 82 2.5 83 1.5 84 1.5 85 2.5 86 1.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 1.0 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44654711838094613 2 0.8183103610279548 3 0.78937274598675 4 0.7735989087682108 5 0.7824504145455207 6 0.7882379375537615 7 0.7785920658733599 8 0.7743932746713028 9 0.7766628915372796 10-11 0.7713860323238835 12-13 0.7771168149104749 14-15 0.778875767981607 16-17 0.7990753580888011 18-19 0.8194451694609433 20-21 0.8163244462702252 22-23 0.7969759624877724 24-25 0.7930608733939624 26-27 0.7944793839351979 28-29 0.8141683102475471 30-31 0.8235304798197016 32-33 0.8215445650619719 34-35 0.8285803773465001 36-37 0.8368644789073157 38-39 0.8273888284918623 40-41 0.8344813811980399 42-43 0.8302258495743333 44-45 0.8184238418712537 46-47 0.8149059357289896 48-49 0.8187075439795009 50-51 0.852524835282556 52-53 0.8240978840361959 54-55 0.8349920449928848 56-57 0.8529787586557513 58-59 0.8144520123557942 60-61 0.8417441551691659 62-63 0.8382829894485512 64-65 0.8293180028279427 66-67 0.8309634750557758 68-69 0.8493473716701884 70-71 0.877036697435106 72-73 0.8785686888196402 74-75 0.8832214033948929 76-77 0.891165062425812 78-79 0.8718733190650088 80-81 0.8901437348361223 82-83 0.8926970538103463 84-85 0.8628515920227506 86-87 0.8517304693794642 88-89 0.8526950565475042 90-91 0.8651212088887275 92-93 0.8550214138351305 94-95 0.850255218416579 96-97 0.8571775498578085 98-99 0.8812354886371632 100-101 0.874199676352635 102-103 0.8532057203423491 104-105 0.8451485804681311 106-107 0.8452053208897806 108-109 0.8452053208897806 110-111 0.8436165890835968 112-113 0.8533192011856477 114-115 0.8553051159433775 116-117 0.8751075230990257 118-119 0.8739727146660372 120-121 0.8566101456413143 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 881206.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.34894810850311 #Duplication Level Percentage of deduplicated Percentage of total 1 87.98496299764723 53.977849292712264 2 7.776877624083429 9.542065236121465 3 1.8121592460879405 3.3352219065777957 4 0.7661588843283617 1.8801216655011717 5 0.435012744481227 1.334378714385816 6 0.2588983630131526 0.9529885342722181 7 0.18171456878031444 0.780359835546377 8 0.12134435925171677 0.5955479039194504 9 0.09566140116019836 0.5281853702187337 >10 0.5105187495429948 5.504936862583811 >50 0.027509607507813942 1.1529777956166718 >100 0.020259735669199575 2.752540209469297 >500 0.0018586913429995576 0.8200948329400962 >1k 0.005947812297598584 7.65326820111797 >5k 3.717382685999115E-4 1.5978849963834216 >10k+ 7.43476537199823E-4 7.59157864263346 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 24431 2.7724504826340266 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 15393 1.746810620899086 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14797 1.6791760382929757 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11955 1.3566634816376648 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 8298 0.9416640376937969 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 5715 0.6485430194528862 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4198 0.47639258016854175 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3516 0.398998645038731 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3488 0.39582118142636347 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3482 0.39514029636657033 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3356 0.38084171011091617 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3263 0.3702879916841238 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2845 0.32285299918520755 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2781 0.3155902252140816 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2656 0.30140511980172624 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2654 0.3011781581151286 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2594 0.294369307517198 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2494 0.2830212231873137 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2358 0.26758782849867113 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2339 0.26543169247599313 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2169 0.24613994911518985 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2100 0.2383097709275697 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1777 0.2016554585420435 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1638 0.1858816213235044 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1492 0.16931341820187334 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1438 0.16318545266373582 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1360 0.1543339468864261 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1331 0.15104300243075966 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1327 0.1505890790575643 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 1325 0.1503621173709666 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1255 0.14241845834004763 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1207 0.13697137786170316 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1183 0.13424783762253095 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1176 0.13345347171943903 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1140 0.1293681613606807 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1116 0.12664462112150848 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1045 0.11858748124729064 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1014 0.11506957510502651 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 991 0.11245951570915313 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 923 0.10474281836483183 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1348084329884272E-4 0.0 10-11 0.0 0.0 0.0 3.404425298965282E-4 0.0 12-13 0.0 5.674042164942136E-5 0.0 4.539233731953709E-4 0.0 14-15 0.0 1.1348084329884272E-4 0.0 5.674042164942136E-4 0.0 16-17 0.0 1.1348084329884272E-4 0.0 6.24144638143635E-4 0.0 18-19 0.0 1.1348084329884272E-4 0.0 9.078467463907418E-4 0.0 20-21 0.0 1.1348084329884272E-4 0.0 0.001588731806183798 0.0 22-23 0.0 1.1348084329884272E-4 0.0 0.0027235402391722254 0.0 24-25 0.0 1.1348084329884272E-4 0.0 0.004085310358758338 0.0 26-27 0.0 1.1348084329884272E-4 0.0 0.005787523008240978 0.0 28-29 0.0 2.2696168659768544E-4 0.0 0.010950901378338321 0.0 30-31 0.0 2.2696168659768544E-4 0.0 0.02553318974223961 0.0 32-33 0.0 2.2696168659768544E-4 0.0 0.0549814685782893 0.0 34-35 0.0 2.2696168659768544E-4 0.0 0.08346516024629883 0.0 36-37 0.0 2.2696168659768544E-4 0.0 0.1088848691452396 0.0 38-39 0.0 2.2696168659768544E-4 0.0 0.14219149665344993 0.0 40-41 0.0 2.2696168659768544E-4 0.0 0.16778142681733896 0.0 42-43 0.0 2.2696168659768544E-4 0.0 0.2560695229038386 0.0 44-45 0.0 2.2696168659768544E-4 0.0 0.34350651266559695 0.0 46-47 0.0 2.2696168659768544E-4 0.0 0.4070557849129488 0.0 48-49 0.0 2.2696168659768544E-4 0.0 0.4795133033592599 0.0 50-51 0.0 3.404425298965282E-4 0.0 0.5615032126426738 0.0 52-53 0.0 3.404425298965282E-4 0.0 0.7549880504672006 0.0 54-55 0.0 3.404425298965282E-4 0.0 0.8735755317144913 0.0 56-57 0.0 3.404425298965282E-4 0.0 1.197393118067739 0.0 58-59 0.0 3.404425298965282E-4 0.0 1.564219944031248 0.0 60-61 0.0 3.404425298965282E-4 0.0 1.7941321325547035 0.0 62-63 0.0 3.404425298965282E-4 0.0 2.1999396281913652 0.0 64-65 0.0 3.404425298965282E-4 0.0 2.3845162198169327 0.0 66-67 0.0 3.404425298965282E-4 0.0 3.0146753426554067 0.0 68-69 0.0 3.404425298965282E-4 0.0 3.603243736424854 0.0 70-71 0.0 3.404425298965282E-4 0.0 3.8221482831483216 0.0 72-73 0.0 4.539233731953709E-4 0.0 4.346656740875573 0.0 74-75 0.0 4.539233731953709E-4 0.0 4.767216746141084 0.0 76-77 0.0 5.106637948447923E-4 0.0 4.917124940138855 0.0 78-79 0.0 5.674042164942136E-4 0.0 5.1296745596375874 0.0 80-81 0.0 5.674042164942136E-4 0.0 5.56390900652061 0.0 82-83 0.0 5.674042164942136E-4 0.0 5.870193802584186 0.0 84-85 0.0 5.674042164942136E-4 0.0 6.6073086202318185 0.0 86-87 0.0 5.674042164942136E-4 0.0 8.194224732922835 0.0 88-89 0.0 5.674042164942136E-4 0.0 10.334984101333854 0.0 90-91 0.0 5.674042164942136E-4 0.0 11.801837481814694 0.0 92-93 0.0 5.674042164942136E-4 0.0 12.924730426256744 0.0 94-95 0.0 6.24144638143635E-4 0.0 13.822534118015538 0.0 96-97 0.0 6.808850597930564E-4 0.0 14.88244519442673 0.0 98-99 0.0 6.808850597930564E-4 0.0 15.819343036701973 0.0 100-101 0.0 7.94365903091899E-4 0.0 16.296019318978765 5.674042164942136E-5 102-103 0.0 7.94365903091899E-4 0.0 16.64066064007735 1.1348084329884272E-4 104-105 0.0 7.94365903091899E-4 0.0 17.051007369445962 1.1348084329884272E-4 106-107 0.0 7.94365903091899E-4 0.0 17.65342042609787 1.1348084329884272E-4 108-109 0.0 7.94365903091899E-4 0.0 18.50838509951135 1.1348084329884272E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1170 0.0 56.01161 2 TATAACG 185 0.0 55.931934 2 ACCTGGG 1210 0.0 54.591316 3 GTACCCG 120 1.0913936E-11 47.987095 1 TACCCGG 100 5.6206773E-9 45.988476 2 ATAACGC 215 0.0 45.41732 3 TAACGCA 220 0.0 41.774223 4 GTACCTG 1675 0.0 39.879425 1 CCTGGGG 815 0.0 36.648552 4 AGTACGC 315 0.0 36.498795 2 GTATAAC 360 0.0 35.190536 1 GTACCGG 100 1.3287445E-5 34.55071 1 CTATACG 70 0.0033143368 32.822605 4 GTACGCA 340 0.0 32.098576 3 GAGTACG 415 0.0 30.52673 1 TACACGG 115 3.4958644E-5 29.992487 2 CGCCGTC 100 2.6902853E-9 28.746914 114-115 GTCCGTC 40 0.006362798 28.727175 28-29 CGCCGTA 370 0.0 27.969973 114-115 TCGCCGT 425 0.0 27.732317 114-115 >>END_MODULE