##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003850_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 881206 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.57081658545221 31.0 31.0 34.0 30.0 34.0 2 31.764000699041993 31.0 31.0 34.0 30.0 34.0 3 31.829401978651983 31.0 31.0 34.0 30.0 34.0 4 35.48881873250976 37.0 35.0 37.0 33.0 37.0 5 35.39908375567121 37.0 35.0 37.0 33.0 37.0 6 35.483976504926204 37.0 35.0 37.0 33.0 37.0 7 35.43498228564036 37.0 35.0 37.0 33.0 37.0 8 35.4529304158165 37.0 35.0 37.0 33.0 37.0 9 36.98406275036711 39.0 37.0 39.0 33.0 39.0 10-11 36.89124620122877 39.0 37.0 39.0 32.5 39.0 12-13 36.84750898200875 39.0 37.0 39.0 32.0 39.0 14-15 37.78461619643988 39.0 37.0 41.0 32.5 41.0 16-17 37.9047424779223 39.5 37.0 41.0 33.0 41.0 18-19 37.986800475711696 40.0 37.0 41.0 33.0 41.0 20-21 37.914872345399374 40.0 37.0 41.0 32.5 41.0 22-23 37.8253467407167 39.5 37.0 41.0 32.0 41.0 24-25 37.66798455752684 39.5 36.5 41.0 32.0 41.0 26-27 37.32165918071371 39.0 36.0 41.0 31.5 41.0 28-29 37.19216902744648 39.0 36.0 40.0 31.0 41.0 30-31 37.05865257385901 39.0 36.0 40.0 31.0 41.0 32-33 36.80669446190788 39.0 35.0 40.0 30.0 41.0 34-35 36.6958163017501 39.0 35.0 40.0 30.0 41.0 36-37 36.46163382909331 38.5 35.0 40.0 30.0 41.0 38-39 36.23802833843619 38.0 35.0 40.0 30.0 41.0 40-41 36.513459962823674 39.0 35.0 40.0 30.0 41.0 42-43 36.40628411517852 39.0 35.0 40.0 30.0 41.0 44-45 36.25408814737984 39.0 35.0 40.5 30.0 41.0 46-47 35.96544735283237 39.0 35.0 40.0 29.0 41.0 48-49 35.666416252272455 38.5 35.0 40.0 28.0 41.0 50-51 35.3108126817112 38.0 34.0 40.0 27.0 41.0 52-53 35.19854267901036 38.0 34.0 40.0 27.0 41.0 54-55 35.03623102884002 38.0 34.0 40.0 26.0 41.0 56-57 34.70463603289129 38.0 33.5 40.0 25.5 41.0 58-59 34.41753063415365 38.0 33.0 40.0 25.0 41.0 60-61 33.820831905366056 37.0 32.5 40.0 22.5 41.0 62-63 33.697010120221606 37.0 32.0 40.0 23.0 41.0 64-65 33.25067123918811 36.5 32.0 39.0 22.0 41.0 66-67 32.869866410351264 36.0 31.5 39.0 21.5 40.0 68-69 32.5065603275511 35.5 31.0 39.0 21.0 40.0 70-71 31.949855652367326 35.0 31.0 38.0 19.0 40.0 72-73 31.49258062246512 35.0 30.5 37.0 18.5 40.0 74-75 31.13677959523653 34.0 30.0 37.0 18.0 39.0 76-77 30.1914302671566 34.0 29.0 36.0 15.0 39.0 78-79 30.58021166446892 34.0 30.0 36.0 14.0 39.0 80-81 30.286047757278094 34.0 30.0 36.0 11.0 37.5 82-83 29.942449325129424 34.0 29.5 35.0 7.5 37.0 84-85 29.624310887579067 34.0 29.0 35.0 7.0 37.0 86-87 29.39433912161288 34.0 29.0 35.0 2.0 36.0 88-89 29.05997916491717 34.0 29.0 35.0 2.0 36.0 90-91 28.70434892635774 34.0 29.0 35.0 2.0 35.5 92-93 28.42517300154561 34.0 29.0 35.0 2.0 35.0 94-95 28.052886044806776 34.0 29.0 35.0 2.0 35.0 96-97 27.53941643611142 33.5 27.0 35.0 2.0 35.0 98-99 27.255334167039262 34.0 27.0 35.0 2.0 35.0 100-101 26.21991679584569 33.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.028321547832465654 1101 2 0.07445220724272161 1101 3 0.07191375060812533 1101 4 0.00905207608198566 1101 5 -0.02067236994681565 1101 6 0.01904436453519054 1101 7 -0.07211557262071011 1101 8 -5.465544862062188E-4 1101 9 0.13682922995939606 1101 10-11 0.13445346683917592 1101 12-13 0.1186658719341338 1101 14-15 0.09952769894273672 1101 16-17 0.21371309976091624 1101 18-19 -0.023860540706131417 1101 20-21 -0.09623000840653617 1101 22-23 -0.0020552437497300957 1101 24-25 0.043938611222287705 1101 26-27 -0.0053944090442925585 1101 28-29 -0.19280066880751434 1101 30-31 0.11305969266904725 1101 32-33 0.08889084993259644 1101 34-35 0.05564245595301287 1101 36-37 0.02917418566794794 1101 38-39 0.09832014989875404 1101 40-41 0.14080704654531218 1101 42-43 -0.2524795926734811 1101 44-45 0.0015250215275983692 1101 46-47 0.037208293862839525 1101 48-49 0.2324556288036277 1101 50-51 0.20319383216657627 1101 52-53 0.1830447590467088 1101 54-55 0.3984670801892065 1101 56-57 0.3125653611348511 1101 58-59 0.16544485492219252 1101 60-61 0.1833571207446596 1101 62-63 0.14949084685608938 1101 64-65 -0.02211183826310048 1101 66-67 0.06939716032935195 1101 68-69 -0.061336627892309536 1101 70-71 -0.010734764114403106 1101 72-73 -0.13388744148891973 1101 74-75 -0.27836657700853706 1101 76-77 -0.16554318619568775 1101 78-79 -0.022162785446830924 1101 80-81 0.005052212400570966 1101 82-83 -0.00216846970274176 1101 84-85 -0.20257694735988352 1101 86-87 -0.0942686940571349 1101 88-89 -0.11052453906667736 1101 90-91 0.026391428840973674 1101 92-93 -0.002209156547486657 1101 94-95 -0.14694535664067487 1101 96-97 -0.1318915064631483 1101 98-99 -0.2647386961399114 1101 100-101 -0.19472452372057703 1102 1 -0.140857316525814 1102 2 -0.08952727375140412 1102 3 -0.07963734527681154 1102 4 -0.045110034236728325 1102 5 -0.1008889190869553 1102 6 -0.052692230719429745 1102 7 -0.0159220274368721 1102 8 0.04797113383174434 1102 9 0.051786282217221924 1102 10-11 -0.028906895779513775 1102 12-13 0.0020003557074801392 1102 14-15 -0.03824778602827905 1102 16-17 0.03988282478153593 1102 18-19 -0.09325871526057483 1102 20-21 -0.05427739684716215 1102 22-23 -0.13665490879004238 1102 24-25 -0.037084456916453234 1102 26-27 -0.03145550830788579 1102 28-29 0.021086716436094832 1102 30-31 -0.04714937255606344 1102 32-33 -0.13807946023814566 1102 34-35 -0.1657824306075426 1102 36-37 -0.34091025662900876 1102 38-39 -0.19277477835617418 1102 40-41 -0.03967367995150539 1102 42-43 0.17734992422649043 1102 44-45 0.17919110570831265 1102 46-47 0.3503922758626885 1102 48-49 0.07782619764172694 1102 50-51 0.18902162219552565 1102 52-53 0.07582193334805964 1102 54-55 0.031544834191223003 1102 56-57 0.3534725860192296 1102 58-59 0.2757245208344088 1102 60-61 0.11637668670181256 1102 62-63 -0.025122938321636923 1102 64-65 0.04621430784762737 1102 66-67 0.10525667828287766 1102 68-69 0.007658287162833233 1102 70-71 0.1176915792665092 1102 72-73 -0.07900539791993921 1102 74-75 0.04636721722739523 1102 76-77 -0.0010662912696126625 1102 78-79 -0.023937793287984732 1102 80-81 -0.0713370044646382 1102 82-83 -0.09894196986198978 1102 84-85 -0.2592106872223887 1102 86-87 -0.33249787623960714 1102 88-89 -0.36015700375725146 1102 90-91 -0.24924521860728888 1102 92-93 -0.28699937078363646 1102 94-95 -0.1633681905740545 1102 96-97 -0.18312544420133747 1102 98-99 -0.22480662054026013 1102 100-101 -0.02138288299936164 1103 1 -0.026563375591756255 1103 2 -0.042698123808186494 1103 3 -0.018903765283461382 1103 4 0.0635130967280233 1103 5 0.04852165550260423 1103 6 0.08136379275580197 1103 7 0.03523796483844421 1103 8 -0.033290025303010395 1103 9 -0.0924735739000937 1103 10-11 0.14124703317709475 1103 12-13 0.07651392026540549 1103 14-15 0.1568384648022345 1103 16-17 0.08350567339098092 1103 18-19 0.1390897209442059 1103 20-21 0.1643764585576406 1103 22-23 -0.027985764368438026 1103 24-25 0.057593110756435806 1103 26-27 0.14041416485015645 1103 28-29 0.018367739440726893 1103 30-31 0.19544681822688403 1103 32-33 -0.032290688497653264 1103 34-35 0.007534574920342152 1103 36-37 -0.1386347517533295 1103 38-39 -0.079661500894332 1103 40-41 -0.03837449696923301 1103 42-43 0.15798000182887506 1103 44-45 0.0959053544724 1103 46-47 -0.1177364335365354 1103 48-49 -0.14564721878075915 1103 50-51 -0.0682793539172124 1103 52-53 -0.014513637215323172 1103 54-55 -0.21615222715237792 1103 56-57 0.08100703859504677 1103 58-59 -0.04573594628583777 1103 60-61 -0.11094199064834243 1103 62-63 -0.253118919813069 1103 64-65 -0.08876721356641326 1103 66-67 -0.08198602108959818 1103 68-69 -0.051030134108493996 1103 70-71 0.04654327162053917 1103 72-73 0.11052333364940736 1103 74-75 -0.033203644541906385 1103 76-77 0.051419725171133024 1103 78-79 0.0029323225752904136 1103 80-81 0.038783306781539295 1103 82-83 0.22448382960439517 1103 84-85 0.023320652473355352 1103 86-87 -0.053771361403406104 1103 88-89 -0.1104269842294201 1103 90-91 -0.24704842462975662 1103 92-93 -0.30807248825808387 1103 94-95 -0.6427342924603963 1103 96-97 -0.3167506143988348 1103 98-99 -0.15712744867093775 1103 100-101 -0.3122841402124905 1104 1 -1.7431967554770722E-4 1104 2 -0.013925052293014772 1104 3 -0.04909121153533036 1104 4 -0.013826729114796876 1104 5 0.09416965469179672 1104 6 -0.03180282588263594 1104 7 0.09087054204855605 1104 8 -0.10818381483647954 1104 9 0.04027041141461041 1104 10-11 0.045811152380906606 1104 12-13 -0.10571313755529133 1104 14-15 -0.044294320782341856 1104 16-17 -0.21069599526866511 1104 18-19 -0.17504366442601338 1104 20-21 -0.24819778666165604 1104 22-23 -0.12132995888959641 1104 24-25 -0.08723697140587916 1104 26-27 0.22884057084831966 1104 28-29 0.09136119928118802 1104 30-31 0.05090414911267516 1104 32-33 0.13136532649017596 1104 34-35 0.16790651765858655 1104 36-37 0.20398693930940937 1104 38-39 0.17484350954708106 1104 40-41 0.16336299054153613 1104 42-43 0.325711609473764 1104 44-45 0.3644610812152038 1104 46-47 0.29909076844450055 1104 48-49 0.36358063093852167 1104 50-51 0.4205020828303674 1104 52-53 0.3510160083060683 1104 54-55 0.3384664627130576 1104 56-57 0.4531528568486536 1104 58-59 0.4953107969083348 1104 60-61 0.5625242636562291 1104 62-63 0.3646050865130448 1104 64-65 0.541989083615654 1104 66-67 0.3747447008692859 1104 68-69 0.45556448264512284 1104 70-71 0.25881558186931386 1104 72-73 0.24026763436211596 1104 74-75 0.4996011513229739 1104 76-77 0.28943327745270153 1104 78-79 0.4037653666047021 1104 80-81 0.4472327965855598 1104 82-83 0.5423906457561003 1104 84-85 0.4152427574865172 1104 86-87 0.34370878251489856 1104 88-89 0.2747053656974785 1104 90-91 0.3333568882760751 1104 92-93 0.17332092045310077 1104 94-95 0.34537626172490477 1104 96-97 0.5286805392090663 1104 98-99 0.44050676376814835 1104 100-101 0.4934178321217324 1105 1 0.14439402932629974 1105 2 0.13911512045159213 1105 3 0.08245813676278502 1105 4 0.2788647064337795 1105 5 0.1567745374937246 1105 6 0.16981274519106648 1105 7 0.1981502355351452 1105 8 0.09643012623065772 1105 9 0.20524391628959648 1105 10-11 0.2048375392398043 1105 12-13 0.2790781795020365 1105 14-15 0.3646914662822667 1105 16-17 0.478019367092017 1105 18-19 0.3651681762784378 1105 20-21 0.41270972399954786 1105 22-23 0.333804261022955 1105 24-25 0.3279741174505304 1105 26-27 0.2360086900853986 1105 28-29 0.2899979484105444 1105 30-31 0.2968426659871497 1105 32-33 0.48532034793288403 1105 34-35 0.47412520318761864 1105 36-37 0.46670219340397523 1105 38-39 0.625988423746179 1105 40-41 0.4745859766413716 1105 42-43 0.572816994510184 1105 44-45 0.5158953065410543 1105 46-47 0.34020482679994046 1105 48-49 0.23565819759737394 1105 50-51 0.3254128535046803 1105 52-53 0.35303697584427596 1105 54-55 0.48779701721602464 1105 56-57 0.5284624678725436 1105 58-59 0.34632007772056994 1105 60-61 0.34741001282769446 1105 62-63 0.3556159913170447 1105 64-65 0.4455179575878745 1105 66-67 0.21028997114361658 1105 68-69 0.15253212912015357 1105 70-71 0.14292873199231337 1105 72-73 0.1314271043393198 1105 74-75 0.25187925089171515 1105 76-77 0.36397149238500504 1105 78-79 0.20528951295643338 1105 80-81 0.2848591822415045 1105 82-83 0.19293468299964545 1105 84-85 0.2543517874822463 1105 86-87 0.3078726974177286 1105 88-89 0.2968502979409351 1105 90-91 0.43958850559143414 1105 92-93 0.3788190348182141 1105 94-95 0.6088238158128547 1105 96-97 0.7146050416360303 1105 98-99 0.7686027236375068 1105 100-101 0.540605318964392 1106 1 -0.07552246291162135 1106 2 -0.014270432487531792 1106 3 -0.005614752649389487 1106 4 -0.03466006244812547 1106 5 -0.011903510648608062 1106 6 -0.0031224545298442763 1106 7 0.13538314151804798 1106 8 0.1685209818036597 1106 9 -0.036901339248515796 1106 10-11 -0.21887441349000625 1106 12-13 -0.21617953896996767 1106 14-15 -0.2530697407646585 1106 16-17 -0.19488448996362706 1106 18-19 -0.11410478290523685 1106 20-21 -0.04228902229384346 1106 22-23 0.01753360211836963 1106 24-25 -0.0648148891777609 1106 26-27 -0.060608754969535994 1106 28-29 -0.17622556022367775 1106 30-31 0.07830661374573822 1106 32-33 0.13183504355471598 1106 34-35 0.0291465707089813 1106 36-37 -0.027908230893949337 1106 38-39 0.06877034420667627 1106 40-41 -0.3048965695822474 1106 42-43 -0.20895765445196446 1106 44-45 -0.2218701522065487 1106 46-47 -0.05001953217442434 1106 48-49 -0.2676845657900344 1106 50-51 0.09931231713628819 1106 52-53 -0.18814319814840275 1106 54-55 -0.0020438710623693623 1106 56-57 0.23740766454096018 1106 58-59 -0.03263046082818022 1106 60-61 -0.017804261984792902 1106 62-63 -0.23159020526414764 1106 64-65 -0.20929573070795726 1106 66-67 -0.21568274165944956 1106 68-69 -0.3730026040038652 1106 70-71 -0.16551852511565812 1106 72-73 -0.02894241205698478 1106 74-75 0.1628347388561302 1106 76-77 -0.3774406173306737 1106 78-79 -0.21305603038910803 1106 80-81 0.028196821784497672 1106 82-83 -0.08212582197157303 1106 84-85 0.05910742371109734 1106 86-87 0.11580620736016556 1106 88-89 0.10868801379650606 1106 90-91 -0.0349012903269319 1106 92-93 0.11236822372454469 1106 94-95 0.16109520513781206 1106 96-97 0.30290550605256783 1106 98-99 -0.18202031403910013 1106 100-101 -0.20256470545563232 1107 1 -0.0582110437106671 1107 2 -0.13040974375765657 1107 3 -0.08921828574420232 1107 4 -0.2328686690869688 1107 5 -0.043141087671074274 1107 6 -0.09151193972165572 1107 7 -0.012444079710334677 1107 8 0.13105017580087264 1107 9 -0.1335210470600856 1107 10-11 -0.0342596500235004 1107 12-13 0.0021045993723092238 1107 14-15 -0.17607212597634714 1107 16-17 -0.11945993470622085 1107 18-19 -0.13425174672952522 1107 20-21 -0.07678297230377495 1107 22-23 -0.24670778875820076 1107 24-25 -0.047282962125485994 1107 26-27 -0.060964362701597 1107 28-29 -0.2771591469857526 1107 30-31 -0.19382336786825505 1107 32-33 -0.19203819238246922 1107 34-35 -0.17962156683822883 1107 36-37 -0.1014041188883823 1107 38-39 0.02083628537145188 1107 40-41 0.08675177319254601 1107 42-43 -0.07659238164337978 1107 44-45 -0.043733971526762616 1107 46-47 0.014623431451099123 1107 48-49 -0.1440153772589241 1107 50-51 0.11547771665924245 1107 52-53 0.06649212534593829 1107 54-55 -0.005927604415575161 1107 56-57 -0.2228558889275405 1107 58-59 0.04660484278938526 1107 60-61 -0.1541324646284039 1107 62-63 -0.3769421482178359 1107 64-65 -0.5001393751126457 1107 66-67 -0.28415974672802946 1107 68-69 -0.3157482062698165 1107 70-71 -0.07520658414964743 1107 72-73 -0.4049595433430113 1107 74-75 -0.2845337639266141 1107 76-77 -0.18619198385820468 1107 78-79 -0.4276008524920698 1107 80-81 -0.44918870047747816 1107 82-83 -0.5988440439950296 1107 84-85 -0.5885132655156973 1107 86-87 -0.39086959019498835 1107 88-89 -0.6863670199939911 1107 90-91 -0.7629374660375277 1107 92-93 -0.5904368846038182 1107 94-95 -0.5411788209007469 1107 96-97 -0.582776701759002 1107 98-99 -0.8026940121110577 1107 100-101 -0.5721669217148246 1108 1 -0.09649949120247925 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2507.0 13 3549.0 14 4171.0 15 5216.0 16 6692.0 17 8336.0 18 7722.0 19 6098.0 20 5808.0 21 5905.0 22 6253.0 23 6862.0 24 7962.0 25 9454.0 26 11356.0 27 14104.0 28 17520.0 29 21695.0 30 26837.0 31 32683.0 32 40021.0 33 49905.0 34 63727.0 35 83889.0 36 118131.0 37 150673.0 38 130545.0 39 29802.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.949516785350966 19.89695356779538 13.66114572736521 18.492383919488447 2 14.305071512306835 26.512040146304315 36.99812863930856 22.184759702080285 3 26.283455664504586 26.663276356445714 28.11422318381914 18.93904479523056 4 12.300075124318264 17.336695392450803 42.42345149715276 27.93977798607817 5 12.092859104454577 41.8317623801926 32.71981806751202 13.3555604478408 6 26.510227056078584 41.25843176061517 16.68569392368519 15.54564725962106 7 28.138029019321248 34.09463848407751 21.46660372262558 16.300728773975663 8 23.545572771860382 32.13743437970236 19.235683824213634 25.081309024223618 9 22.63886083390263 24.51174867170673 22.485207772075995 30.36418272231465 10-11 25.31712437585111 25.85559464366772 28.074046754425787 20.75323422605538 12-13 30.818616759304863 23.777470875141567 25.837715585232058 19.566196780321512 14-15 22.536732120788443 27.72814088429453 29.424281387280875 20.31084560763615 16-17 18.894276707149064 29.477897336150683 30.092112400505673 21.53571355619458 18-19 18.89887268130267 34.38015628581739 28.143986763594437 18.5769842692855 20-21 22.283408713021867 27.76301079906253 26.998167053495937 22.955413434419672 22-23 19.83123128984596 29.43443417316723 27.073465228334804 23.660869308652007 24-25 24.15377335152053 29.328329584682812 27.11806319975125 19.39983386404541 26-27 23.90634637722361 25.816881643733318 31.932705397152894 18.344066581890175 28-29 19.824608492993818 32.64411152012593 28.860607787069668 18.670672199810582 30-31 22.03602675222415 28.900108157430914 27.476356876395236 21.5875082139497 32-33 22.26291951468904 29.656807392923245 28.0413928347294 20.03888025765831 34-35 24.16403516984858 26.703947387086796 29.660302842016563 19.47171460104806 36-37 23.727289907703938 26.145386587304426 31.34536138933865 18.781962115652988 38-39 21.40727185030713 28.989800116326908 31.176916202068348 18.42601183129761 40-41 20.096751861267478 30.18760214272744 29.121856273833302 20.59378972217178 42-43 19.34485239546712 31.35197672280942 26.33731499785521 22.965855883868244 44-45 22.42143153814205 27.47280431590343 26.66413982655588 23.44162431939864 46-47 24.286176315386317 30.108033885418262 26.377914333214182 19.227875465981242 48-49 23.97719715934753 29.70366747389373 27.016895027950333 19.302240338808403 50-51 20.25218847806302 29.978120893411987 31.089268570572603 18.6804220579524 52-53 22.05040592097648 32.121093138267334 26.484159209083913 19.344341731672277 54-55 19.712133144803826 30.380637444592978 27.10819036638425 22.799039044218947 56-57 23.16074794566874 27.42624437264735 27.923755116677846 21.489252565006066 58-59 26.59868010056701 26.94037188912663 26.401449835367202 20.05949817493916 60-61 21.638134386669943 29.01133933278215 30.551055369968218 18.79947091057969 62-63 20.241042755636922 28.359182987861036 30.168138614906447 21.231635641595595 64-65 19.98759053065525 33.4379566270313 27.378377549873463 19.19607529243999 66-67 21.519622421343456 27.891886553868297 26.775234908318218 23.81325611647003 68-69 20.040842473903204 30.404946490004146 26.298796911678007 23.255414124414642 70-71 23.67021502954195 29.740542632272078 25.802807407361172 20.786434930824797 72-73 23.023390671420756 26.923500293915385 29.992986883884132 20.060122150779726 74-75 20.418721615604067 32.84901600760776 27.13100001588732 19.601262360900858 76-77 21.96120997814359 28.55541156097439 26.546914115428173 22.936464345453842 78-79 21.817543230527257 29.883250908414148 26.821764717897974 21.477441143160622 80-81 23.369166800952332 28.189208879649026 27.536013145620885 20.905611173777753 82-83 22.602093040673804 27.21032312535321 29.477613634042438 20.70997019993055 84-85 20.436209261063397 29.799235252163086 28.65733361854868 21.107221868224833 86-87 20.428599312752212 29.58938760502771 26.7569303537465 23.225082728473577 88-89 20.04968191319623 30.182443151771547 25.700971169056952 24.066903765975265 90-91 21.90532066281891 25.821885007591867 26.97927612839677 25.293518201192455 92-93 22.749334434854052 29.053138539683115 26.554574072350846 21.642952953111987 94-95 22.0907608951601 29.11091680657917 27.252837164543344 21.545485133717392 96-97 18.598821381273968 29.822594391064012 30.866226888076614 20.71235733958541 98-99 21.152868468296713 32.25923227591404 25.552427252775185 21.035472003014064 100-101 18.166121477429733 30.98352516618106 25.950686144060462 24.899667212328747 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 148.0 1 108.0 2 142.0 3 216.5 4 185.5 5 173.0 6 217.0 7 324.0 8 502.0 9 609.0 10 639.0 11 671.5 12 701.0 13 773.5 14 875.5 15 993.0 16 1079.0 17 1169.0 18 1263.5 19 1343.0 20 1496.0 21 1731.5 22 2031.0 23 2382.0 24 2886.0 25 3539.5 26 4498.5 27 5922.0 28 8005.5 29 10519.5 30 13009.5 31 15501.0 32 17559.5 33 19191.0 34 20738.5 35 21775.5 36 22607.0 37 23969.0 38 25164.5 39 25350.5 40 25218.5 41 25478.5 42 25670.5 43 26290.0 44 27156.5 45 27810.5 46 31416.0 47 51348.5 48 98170.5 49 95148.5 50 48706.5 51 29383.5 52 20698.5 53 16616.5 54 14409.0 55 12586.5 56 10540.5 57 8543.0 58 6776.5 59 5366.0 60 4192.5 61 3142.0 62 2358.5 63 1655.0 64 1057.5 65 677.5 66 397.0 67 205.5 68 107.5 69 55.5 70 32.5 71 23.5 72 14.0 73 6.0 74 3.0 75 2.5 76 1.0 77 1.5 78 1.5 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04970460936489312 2 0.0032909444556664387 3 0.02995894263089448 4 0.0 5 0.0 6 0.001588731806183798 7 0.0 8 0.0 9 0.0 10-11 6.808850597930564E-4 12-13 0.0 14-15 0.0032909444556664387 16-17 0.0 18-19 0.0 20-21 0.012596373606171541 22-23 0.0 24-25 0.0 26-27 0.007432995236074199 28-29 0.01038349716184411 30-31 0.009418909993803945 32-33 0.0044257528886548665 34-35 0.006071225116488085 36-37 0.020710253902038796 38-39 0.007773437765970727 40-41 0.009759352523700474 42-43 0.0 44-45 0.0 46-47 1.1348084329884273E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0021561360226780117 58-59 2.837021082471068E-4 60-61 0.007886918609269569 62-63 0.013333999087614021 64-65 0.04845632008860584 66-67 0.025419708898940768 68-69 0.001758953071132062 70-71 1.702212649482641E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 5.674042164942137E-5 86-87 0.0011348084329884272 88-89 0.0 90-91 0.0 92-93 0.0 94-95 5.674042164942137E-5 96-97 5.674042164942136E-4 98-99 4.5392337319537094E-4 100-101 0.001531991384534377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 881206.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.81704891581455 #Duplication Level Percentage of deduplicated Percentage of total 1 85.68902764279288 48.685976751291484 2 9.188504084657119 10.441273720822505 3 2.1822616910266954 3.7196900773851587 4 0.9279799595614677 2.1090033102119805 5 0.48947592087369834 1.3905288669697147 6 0.3010495141182075 1.026284698184184 7 0.21161725317795876 0.8416427477669677 8 0.16042685577856297 0.729198440974475 9 0.12861616749155053 0.6576831971758822 >10 0.6478283971085479 6.560331270160239 >50 0.03960710074818204 1.5577424831677158 >100 0.021403447357844434 2.2552046383282587 >500 0.004800773235645869 1.842646413645168 >1k 0.005400869890101602 6.490120486720984 >5k 0.0016002577452152895 5.517255915031436 >10k+ 4.000644363038224E-4 6.175416982163933 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 43385 4.923366386520291 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10951 1.2427287149656268 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 7816 0.8869662712237547 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6503 0.7379659239723741 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6269 0.711411406640445 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6253 0.7095957131476636 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6092 0.6913252973765499 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 5390 0.6116617453807623 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5136 0.5828376111828563 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 5086 0.5771635690179141 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4542 0.5154299902633436 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4165 0.47264771233967995 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3917 0.44450446320156695 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3397 0.38549442468616874 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3054 0.34657049543466567 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2748 0.3118453573852198 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2553 0.28971659294194546 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2420 0.2746236407831994 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2339 0.26543169247599313 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2287 0.2595306886244533 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2174 0.2467073533316841 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2146 0.24352988971931647 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2045 0.23206832454613335 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1992 0.22605383985129468 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1948 0.2210606827461456 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1465 0.1662494354328046 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1392 0.15796533387198908 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1382 0.15683052543900064 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1337 0.1517238874905527 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1328 0.15070255990086312 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1309 0.14854642387818512 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1308 0.14843294303488627 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1294 0.14684421122870248 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1273 0.14446111351942678 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1142 0.12959512304727838 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1112 0.1261906977483131 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1036 0.11756615365760105 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 988 0.11211907317925662 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 910 0.10326756740194687 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1348084329884272E-4 0.0 10-11 0.0 0.0 0.0 4.539233731953709E-4 0.0 12-13 0.0 0.0 0.0 5.674042164942136E-4 0.0 14-15 0.0 0.0 0.0 6.808850597930564E-4 0.0 16-17 0.0 0.0 0.0 6.808850597930564E-4 0.0 18-19 0.0 0.0 0.0 9.078467463907418E-4 0.0 20-21 0.0 0.0 0.0 0.001588731806183798 0.0 22-23 0.0 0.0 0.0 0.002837021082471068 0.0 24-25 0.0 0.0 0.0 0.0044257528886548665 0.0 26-27 0.0 0.0 0.0 0.006184705959786929 0.0 28-29 0.0 0.0 0.0 0.011518305594832537 0.0 30-31 0.0 0.0 0.0 0.026724738596877462 0.0 32-33 0.0 0.0 0.0 0.05747804713086384 0.0 34-35 0.0 0.0 0.0 0.08721002807516064 0.0 36-37 0.0 0.0 0.0 0.1134241028771933 0.0 38-39 0.0 0.0 0.0 0.14814924092663917 0.0 40-41 0.0 0.0 0.0 0.17430657530702243 0.0 42-43 0.0 0.0 0.0 0.2648642882594989 0.0 44-45 0.0 0.0 0.0 0.3542304523573375 0.0 46-47 0.0 0.0 0.0 0.4196521585191204 0.0 48-49 0.0 0.0 0.0 0.4929607832901728 0.0 50-51 0.0 0.0 0.0 0.5780146753426554 0.0 52-53 0.0 0.0 0.0 0.7765494106939808 0.0 54-55 0.0 0.0 0.0 0.8966688833258059 0.0 56-57 0.0 0.0 0.0 1.2251959246759554 0.0 58-59 0.0 0.0 0.0 1.5997961884054352 0.0 60-61 0.0 0.0 0.0 1.8333397639144535 0.0 62-63 0.0 0.0 0.0 2.248679650388218 0.0 64-65 0.0 0.0 0.0 2.437284811950895 0.0 66-67 0.0 0.0 0.0 3.0795863850223446 0.0 68-69 0.0 0.0 0.0 3.682793807577343 0.0 70-71 0.0 0.0 0.0 3.910776821764718 0.0 72-73 0.0 0.0 0.0 4.454860724961019 0.0 74-75 0.0 5.674042164942136E-5 0.0 4.893804626840943 0.0 76-77 0.0 1.1348084329884272E-4 0.0 5.051826701134582 0.0 78-79 0.0 1.1348084329884272E-4 0.0 5.271412132917842 0.0 80-81 0.0 1.1348084329884272E-4 0.0 5.727775344244138 0.0 82-83 0.0 1.1348084329884272E-4 0.0 6.046316071383989 0.0 84-85 0.0 1.1348084329884272E-4 0.0 6.806580981064586 0.0 86-87 0.0 1.1348084329884272E-4 0.0 8.448932485707088 0.0 88-89 0.0 1.1348084329884272E-4 0.0 10.653524828473707 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1145 0.0 57.271217 2 ACCTGGG 1270 0.0 49.763474 3 ATAACGC 165 0.0 48.958458 3 TATAACG 185 0.0 43.665657 2 CCTGGGG 850 0.0 38.55196 4 GTACCTG 1735 0.0 37.817173 1 AGTACGC 545 0.0 35.747864 2 GAGTACG 605 0.0 35.36442 1 ACCGATC 55 0.0025815528 34.539307 8 TACGCAG 550 0.0 34.539303 4 GTACCCG 85 1.6211128E-5 33.56153 1 GTACGCA 575 0.0 33.05635 3 ACGCAGT 620 0.0 32.17169 5 CTGGGGG 550 0.0 31.948854 5 GTATAAC 345 0.0 30.318869 1 CGCAGTG 660 0.0 30.221893 6 TACCGTG 110 2.7362894E-6 30.221891 7 TAACGCA 275 0.0 29.358408 4 TCGGACA 65 0.0058484282 29.225567 8 CGCGTCA 35 0.008400056 27.138025 70-71 >>END_MODULE