FastQCFastQC Report
Wed 25 May 2016
SRR1003849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1124056
Sequences flagged as poor quality0
Sequence length121
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT259202.305934935625983No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT232242.0660892339883423No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC157971.4053570284754495No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT152781.35918495163942No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT101900.9065384642758013No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC72300.6432063882938216No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT68370.6082437173948628No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC51680.45976357049826694No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC47740.4247119360601251No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG44720.39784494722682856No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA42320.3764936978228843No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT41760.3715117396286306No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC38860.34571231326553126No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA37050.3296099126733899No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA35600.31671019949184026No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC34680.30852555388699493No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG32800.29180040852057193No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC30870.2746304454582334No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA29070.2586170084052752No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT28680.2551474303771342No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA26710.23762161315806332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.2158255460582035No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC22960.20426028596440038No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22840.20319272349420314No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG22460.19981210900524526No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT19950.17748226067028688No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18680.16618389119403304No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG17350.15435174048268058No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT15940.14180788145786333No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14890.13246670984363768No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA14110.12552755378735578No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT13950.12410413716042618No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG12780.11369540307600333No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12010.10684521055890454No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC11380.10124050759036916No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG1501.8189894E-1242.2634051
TACCTGG9800.040.5063862
ACCTGGG10850.034.403563
TACCGCC1704.783942E-1033.7793548
GTACTAG2850.030.3325881
TACCCGG2355.0567905E-1026.9293172
CCACCCG1308.128596E-526.5002339
GTACCTG16000.025.9343621
ACCCGGG2751.1641532E-1025.0592653
GTACATG44650.024.0079211
TATACCG2753.1832315E-922.975126
TAACCGT1502.1438948E-422.9720257
GGCATCG2553.607238E-822.5215937
ACGCGCT1802.8620761E-522.3339127
CGTTAGG1555.12955E-1022.2785170-71
ACATGGG41500.022.1407173
TACATGG43050.022.0501372
CAATGCG1607.8034645E-1021.53965818-19
CATGGGG13800.021.22324
GTACTTT31300.021.0163114-15
Produced by FastQC (version 0.11.5)