##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003849_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1124056 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.603967240066332 31.0 31.0 34.0 30.0 34.0 2 31.818008177528522 31.0 31.0 34.0 30.0 34.0 3 31.87603998377305 31.0 31.0 34.0 30.0 34.0 4 35.56703135786829 37.0 35.0 37.0 33.0 37.0 5 35.467083490502255 37.0 35.0 37.0 33.0 37.0 6 35.525684663397556 37.0 35.0 37.0 33.0 37.0 7 35.468808493527014 37.0 35.0 37.0 33.0 37.0 8 35.472859893101415 37.0 35.0 37.0 33.0 37.0 9 37.01759520877963 39.0 37.0 39.0 33.0 39.0 10-11 36.941091458076826 39.0 37.0 39.0 33.0 39.0 12-13 36.89230785654807 39.0 37.0 39.0 33.0 39.0 14-15 37.85740834976193 39.0 37.0 41.0 33.0 41.0 16-17 37.94845719430349 40.0 37.0 41.0 33.0 41.0 18-19 38.00536850477201 40.0 37.0 41.0 33.0 41.0 20-21 37.92864412449202 40.0 37.0 41.0 32.5 41.0 22-23 37.84024327969425 39.5 37.0 41.0 32.5 41.0 24-25 37.6928698392251 39.5 36.5 41.0 32.0 41.0 26-27 37.374597439985195 39.0 36.0 41.0 31.5 41.0 28-29 37.26484934914275 39.0 36.0 40.0 31.0 41.0 30-31 37.10586216345094 39.0 36.0 40.0 31.0 41.0 32-33 36.86507656202182 39.0 35.0 40.0 30.0 41.0 34-35 36.78936147309387 39.0 35.0 40.0 30.0 41.0 36-37 36.57365246927199 38.5 35.0 40.0 30.0 41.0 38-39 36.339138352537596 38.0 35.0 40.0 30.0 41.0 40-41 36.64344570021422 39.0 35.0 40.0 30.0 41.0 42-43 36.5378980228743 39.0 35.0 40.0 30.0 41.0 44-45 36.386389112286224 39.0 35.0 40.0 30.0 41.0 46-47 36.10482796230792 39.0 35.0 40.0 29.5 41.0 48-49 35.79004070971553 39.0 35.0 40.0 28.0 41.0 50-51 35.47828800344467 38.0 34.0 40.0 28.0 41.0 52-53 35.37055360231163 38.0 34.0 40.0 27.0 41.0 54-55 35.18393523098493 38.0 34.0 40.0 27.0 41.0 56-57 34.86829570768717 38.0 34.0 40.0 26.0 41.0 58-59 34.55338746468148 38.0 34.0 40.0 26.0 41.0 60-61 33.979673610567446 37.0 33.0 40.0 23.5 41.0 62-63 33.8551904887301 37.0 33.0 40.0 24.0 41.0 64-65 33.392190869494044 36.5 32.0 39.0 22.5 41.0 66-67 32.98315075049642 36.0 32.0 39.0 22.0 40.0 68-69 32.621813770844156 35.5 31.0 38.5 22.0 40.0 70-71 32.08849247724312 35.0 31.0 37.5 20.5 40.0 72-73 31.644770812130353 35.0 30.5 37.0 20.5 39.5 74-75 31.302925743913114 34.5 31.0 37.0 19.5 39.0 76-77 30.3616483520394 34.0 29.5 35.5 18.0 38.5 78-79 30.74164276512914 34.0 30.0 36.0 18.0 38.5 80-81 30.467699563011095 34.0 30.0 35.5 17.0 37.0 82-83 30.121667870639897 34.0 29.5 35.0 14.0 37.0 84-85 29.79560359982065 34.0 29.0 35.0 9.5 36.5 86-87 29.59518653874896 34.0 29.0 35.0 7.0 36.0 88-89 29.2749498245639 34.0 29.0 35.0 4.5 36.0 90-91 28.918567669226444 34.0 29.0 35.0 2.0 35.0 92-93 28.638703053940375 34.0 29.0 35.0 2.0 35.0 94-95 28.223598735294328 34.0 29.0 35.0 2.0 35.0 96-97 27.740434640267033 33.5 27.0 35.0 2.0 35.0 98-99 27.42325204438213 34.0 27.0 35.0 2.0 35.0 100-101 26.36155494032326 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.12462928745728163 1101 2 0.1340797400930107 1101 3 0.18527399259314947 1101 4 0.2505464854960664 1101 5 0.31445857825352874 1101 6 0.3015909074507164 1101 7 0.22417075831607036 1101 8 0.17145418767071874 1101 9 0.371529346270691 1101 10-11 0.40724596977084815 1101 12-13 0.3578134310515466 1101 14-15 0.5582048105403388 1101 16-17 0.46103807852454537 1101 18-19 0.25732924122910106 1101 20-21 0.3572960948089232 1101 22-23 0.45999741524935445 1101 24-25 0.3197846599710843 1101 26-27 0.5709871358875347 1101 28-29 0.4013428757194646 1101 30-31 0.4913001133204844 1101 32-33 0.5881974886105397 1101 34-35 0.5515325493272698 1101 36-37 0.640770597129702 1101 38-39 0.5787377260922497 1101 40-41 0.6086380434062733 1101 42-43 0.2733272445953432 1101 44-45 0.670254659219971 1101 46-47 0.7409422715209502 1101 48-49 0.6548281384535812 1101 50-51 0.7031452753440064 1101 52-53 0.7774725201636414 1101 54-55 0.7056115826730007 1101 56-57 0.8768299738763972 1101 58-59 0.7737087040069355 1101 60-61 0.7707536786589415 1101 62-63 0.48592823248925043 1101 64-65 0.4176598805488183 1101 66-67 0.5319432746304287 1101 68-69 0.41412937284815854 1101 70-71 0.5267277106164165 1101 72-73 0.6046976649208631 1101 74-75 0.6644340063396683 1101 76-77 0.6859154960542035 1101 78-79 0.6691147476225616 1101 80-81 0.6641450461473113 1101 82-83 0.7054335988905898 1101 84-85 0.6689648605150573 1101 86-87 0.6141360762213175 1101 88-89 0.8174601458383464 1101 90-91 0.8948257697157374 1101 92-93 0.962091150893329 1101 94-95 0.8778661026271593 1101 96-97 1.0435487766008613 1101 98-99 0.8276212467549193 1101 100-101 1.0675865635971853 1102 1 0.040069493000931544 1102 2 0.1727714338463322 1102 3 0.14467696861701285 1102 4 0.13376349672019927 1102 5 4.6556241370865337E-4 1102 6 0.20981032450579562 1102 7 1.200069278297633E-4 1102 8 0.08007325828614142 1102 9 0.18957953783635162 1102 10-11 0.22984922386043394 1102 12-13 0.2628631933027208 1102 14-15 0.37499617028962007 1102 16-17 0.24298575576617054 1102 18-19 0.07668561224294734 1102 20-21 0.1647733734384289 1102 22-23 0.03847682902635796 1102 24-25 0.04442873980335804 1102 26-27 0.29328075184484703 1102 28-29 0.33830571079458593 1102 30-31 0.1424116731294447 1102 32-33 0.1919002272151431 1102 34-35 0.2103871496934815 1102 36-37 0.27054221676145573 1102 38-39 0.37747228098716334 1102 40-41 0.42138925500031377 1102 42-43 0.43866396645184835 1102 44-45 0.3259786034117198 1102 46-47 0.5266206345540283 1102 48-49 0.5733123243445633 1102 50-51 0.6684717586870832 1102 52-53 0.7219528628770107 1102 54-55 0.6096734162633268 1102 56-57 0.7167561994550198 1102 58-59 0.6392241823981806 1102 60-61 0.8136341547805799 1102 62-63 0.7867806876012793 1102 64-65 0.7554006449043769 1102 66-67 0.6903281337585909 1102 68-69 0.7559651031507713 1102 70-71 0.8399643727555777 1102 72-73 0.8140014416140531 1102 74-75 0.8286446015752027 1102 76-77 0.7696642288733848 1102 78-79 0.8705856221278303 1102 80-81 0.9373320932413982 1102 82-83 0.877116221672555 1102 84-85 0.7309566424602032 1102 86-87 0.609142164401721 1102 88-89 0.7443800099165863 1102 90-91 0.8320107321957622 1102 92-93 0.9189811870620836 1102 94-95 0.9650135385386811 1102 96-97 1.1617013313296702 1102 98-99 1.0076568266876116 1102 100-101 1.0906979203935592 1103 1 0.10806169563706902 1103 2 0.07647375184627947 1103 3 0.10645058292870146 1103 4 0.09413349021445327 1103 5 0.22545454077316407 1103 6 0.23201708333323978 1103 7 0.1430615100174677 1103 8 0.08484151786781524 1103 9 0.11479824293061114 1103 10-11 0.18926742422313225 1103 12-13 0.2636809394546802 1103 14-15 0.38013964226129104 1103 16-17 0.14860226463472515 1103 18-19 0.1798126601829111 1103 20-21 0.23938833093659895 1103 22-23 0.23439024301823252 1103 24-25 0.30658042617394443 1103 26-27 0.41146407568567156 1103 28-29 0.226005083335572 1103 30-31 0.40260377138766756 1103 32-33 0.42680415425545704 1103 34-35 0.28276072253996887 1103 36-37 0.30879553099855883 1103 38-39 0.4322890357872353 1103 40-41 0.4314336036988067 1103 42-43 0.45709870284994736 1103 44-45 0.5198854830601718 1103 46-47 0.6833661168729392 1103 48-49 0.8002779296768949 1103 50-51 0.7743218071822966 1103 52-53 0.7823499040729232 1103 54-55 0.7047767098869784 1103 56-57 0.7558389368254055 1103 58-59 0.7847020926474713 1103 60-61 0.9025057532639948 1103 62-63 0.5834526287683559 1103 64-65 0.7763540346843953 1103 66-67 0.8243568829632437 1103 68-69 0.7720398977907976 1103 70-71 0.8508784182576754 1103 72-73 1.01666117199494 1103 74-75 0.975222888585872 1103 76-77 0.8364099733286032 1103 78-79 0.913250690557323 1103 80-81 0.9162270218569155 1103 82-83 0.8483506930746749 1103 84-85 0.6314463607589893 1103 86-87 0.7058160120147328 1103 88-89 0.5955838113697745 1103 90-91 0.6681721986339753 1103 92-93 0.8012878198257916 1103 94-95 0.7258359554831699 1103 96-97 0.9059929555977746 1103 98-99 0.7231001111126609 1103 100-101 0.8039560603219265 1104 1 -0.003434168577353347 1104 2 -0.060172491877438006 1104 3 -0.10197820951127312 1104 4 0.002623719037053718 1104 5 0.036036678424174795 1104 6 -0.012460902893842274 1104 7 -0.07787182033713691 1104 8 -0.07554067228337402 1104 9 -0.09360541644374365 1104 10-11 -0.06136844233704153 1104 12-13 -0.03637407403339665 1104 14-15 0.12110961104043128 1104 16-17 -0.05421934086550806 1104 18-19 -0.026503558463588206 1104 20-21 -0.04547081219342175 1104 22-23 -0.06440686053557698 1104 24-25 0.12551916851534628 1104 26-27 0.11228592693143469 1104 28-29 0.04481411113274447 1104 30-31 0.004525613138717688 1104 32-33 0.09189907346661386 1104 34-35 0.10377685238297119 1104 36-37 0.257550746109672 1104 38-39 0.17349399872992421 1104 40-41 0.19652628885968682 1104 42-43 0.34635752874482506 1104 44-45 0.3101850971279916 1104 46-47 0.4143886975361184 1104 48-49 0.2058227028370112 1104 50-51 0.2684469530937079 1104 52-53 0.474667842938139 1104 54-55 0.3387866346553281 1104 56-57 0.3451597576154768 1104 58-59 0.5525577394888046 1104 60-61 0.763144021428225 1104 62-63 0.44084649741736825 1104 64-65 0.42099161587815814 1104 66-67 0.2798131318811272 1104 68-69 0.4077278735736911 1104 70-71 0.5570893572848625 1104 72-73 0.7416994301059354 1104 74-75 0.5456492291556643 1104 76-77 0.3800090003997063 1104 78-79 0.4921133634867765 1104 80-81 0.6446169138964315 1104 82-83 0.7113611037489669 1104 84-85 0.6406398965345126 1104 86-87 0.4758047579864595 1104 88-89 0.6419171763521554 1104 90-91 0.6150336372506473 1104 92-93 0.4474381323135681 1104 94-95 0.6388695444290882 1104 96-97 0.7442111329478536 1104 98-99 0.7449805391566926 1104 100-101 0.8547296002304385 1105 1 0.12694669597143715 1105 2 0.21085550882895987 1105 3 0.2770820891367656 1105 4 0.2958276120574794 1105 5 0.18992479333106616 1105 6 0.11076477063401313 1105 7 0.13408489335711238 1105 8 0.23311953217869075 1105 9 0.10496119070261756 1105 10-11 0.4029095632748678 1105 12-13 0.25477342513266166 1105 14-15 0.2258463808538309 1105 16-17 0.367760330660893 1105 18-19 0.2591811539748434 1105 20-21 0.24922092494921344 1105 22-23 0.4005535028653213 1105 24-25 0.21601178080268824 1105 26-27 0.3574873475699363 1105 28-29 0.4831772373223302 1105 30-31 0.5110342566644093 1105 32-33 0.4422552141961589 1105 34-35 0.5327885863860757 1105 36-37 0.5935606945036085 1105 38-39 0.558756345922987 1105 40-41 0.5116480438584148 1105 42-43 0.5678775530762081 1105 44-45 0.5688143968159949 1105 46-47 0.37161649024847065 1105 48-49 0.3074400526790555 1105 50-51 0.47300663364627127 1105 52-53 0.6530560853885419 1105 54-55 0.4839757914946077 1105 56-57 0.33768619836661173 1105 58-59 0.3206865780579591 1105 60-61 0.29859032560968757 1105 62-63 0.30350568153133395 1105 64-65 0.23171949688987326 1105 66-67 0.30246927457288564 1105 68-69 0.27614368504476516 1105 70-71 0.4589377571043336 1105 72-73 0.5225772237994022 1105 74-75 0.5789778204866316 1105 76-77 0.32337526612190004 1105 78-79 0.613914964648405 1105 80-81 0.7138686259228031 1105 82-83 0.547641836829829 1105 84-85 0.5384858828383017 1105 86-87 0.5875206106638906 1105 88-89 0.5337249584685928 1105 90-91 0.7322166916234281 1105 92-93 0.4921897167964282 1105 94-95 0.6110057020336264 1105 96-97 0.7681304469599795 1105 98-99 0.8917443678167025 1105 100-101 0.9587285798823437 1106 1 -0.0029665608409708 1106 2 -0.048585479517665675 1106 3 0.06248470019433228 1106 4 -0.08654336840339738 1106 5 0.14494976485476485 1106 6 0.007820759418727619 1106 7 0.012206333819747783 1106 8 0.09877273466707948 1106 9 0.05206537014711188 1106 10-11 -0.19087822258335763 1106 12-13 -0.2822589064190808 1106 14-15 -0.048761522414373815 1106 16-17 -0.15406127627856137 1106 18-19 0.035164681531142605 1106 20-21 -0.0762412672877062 1106 22-23 -0.09761963303233756 1106 24-25 -0.07058245384201456 1106 26-27 0.0329320159305766 1106 28-29 0.17851150526239934 1106 30-31 0.051382981802412075 1106 32-33 0.1924786436187489 1106 34-35 0.06881329505163336 1106 36-37 -0.10382386696139179 1106 38-39 0.08707492999378275 1106 40-41 -0.03131173884552396 1106 42-43 -0.1515914734724717 1106 44-45 -0.24209421616919968 1106 46-47 -0.2468668182593703 1106 48-49 -0.3851648715301792 1106 50-51 -0.28221110051652687 1106 52-53 -0.30721290611237606 1106 54-55 -0.2824966522099359 1106 56-57 -0.1257108050505451 1106 58-59 -0.3967715836874106 1106 60-61 -0.26908688458920693 1106 62-63 -0.37185574841696933 1106 64-65 -0.3490655727675289 1106 66-67 -0.4041153219398623 1106 68-69 -0.37896890586034715 1106 70-71 -0.6529784787670394 1106 72-73 -0.5121813848048227 1106 74-75 -0.38611461796075375 1106 76-77 -0.43155770505132196 1106 78-79 -0.3760876639330384 1106 80-81 -0.3692033095893308 1106 82-83 -0.26078714766153865 1106 84-85 -0.4213406307865135 1106 86-87 -0.09719418824962744 1106 88-89 0.029628752387807822 1106 90-91 0.02484352067801865 1106 92-93 -0.04390508737425236 1106 94-95 0.05654149455093105 1106 96-97 0.28692918524088995 1106 98-99 0.29782515288635736 1106 100-101 0.22117929398030256 1107 1 -0.3421321010112237 1107 2 -0.25084212584650345 1107 3 -0.2308683620491614 1107 4 -0.21380458160958682 1107 5 -0.29054522375848535 1107 6 -0.2672854965943259 1107 7 -0.31605715673595114 1107 8 -0.26069775112689086 1107 9 -0.5208962802614678 1107 10-11 -0.28212564990911915 1107 12-13 -0.2910254841838693 1107 14-15 -0.33756293023043327 1107 16-17 -0.2864434812887282 1107 18-19 -0.2840984071393464 1107 20-21 -0.20689049457206465 1107 22-23 -0.3548748788521223 1107 24-25 -0.4105347147507601 1107 26-27 -0.5414812925589416 1107 28-29 -0.5107478081228152 1107 30-31 -0.5177741553135107 1107 32-33 -0.6407907534316521 1107 34-35 -0.4974642736646544 1107 36-37 -0.41887385904546903 1107 38-39 -0.4060985017405301 1107 40-41 -0.35841585147080934 1107 42-43 -0.327667734235078 1107 44-45 -0.5887871880494089 1107 46-47 -0.5593624036112672 1107 48-49 -0.5437317245254434 1107 50-51 -0.5535725171009958 1107 52-53 -0.7974751057640788 1107 54-55 -0.7342692095394696 1107 56-57 -0.706167005652155 1107 58-59 -0.6874630698101996 1107 60-61 -0.8035643729284487 1107 62-63 -1.028223193279537 1107 64-65 -1.006236179127061 1107 66-67 -0.9274886002271003 1107 68-69 -0.9456623648741456 1107 70-71 -0.9391067410397618 1107 72-73 -0.9735857909280909 1107 74-75 -1.0733145741187329 1107 76-77 -1.205431513096336 1107 78-79 -1.1059306851355295 1107 80-81 -1.1282402464933519 1107 82-83 -1.023911135337059 1107 84-85 -1.0590813052229606 1107 86-87 -1.1093493890095552 1107 88-89 -1.2955901532665948 1107 90-91 -1.0658272622674367 1107 92-93 -1.132010503081876 1107 94-95 -1.1106610190583623 1107 96-97 -1.0441974329559862 1107 98-99 -1.1646016248467639 1107 100-101 -1.1887156069489038 1108 1 -0.2750083208909011 1108 2 -0.2758600121285184 1108 3 -0.4373535930905845 1108 4 -0.2816808191112159 1108 5 -0.30367005414719017 1108 6 -0.18680603850461353 1108 7 -0.12143149987766577 1108 8 -0.2638503184691814 1108 9 -0.28433566250629383 1108 10-11 -0.42619128110984406 1108 12-13 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86-87 -0.01854540404663041 2316 88-89 -0.07081521320950301 2316 90-91 0.01892243138334493 2316 92-93 0.07341885890405209 2316 94-95 -0.036403353404782735 2316 96-97 -0.267429665278307 2316 98-99 -0.2079659046434088 2316 100-101 -0.31024566840336476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 7.0 5 20.0 6 51.0 7 129.0 8 345.0 9 686.0 10 1127.0 11 1860.0 12 2553.0 13 3639.0 14 4632.0 15 6148.0 16 7868.0 17 10165.0 18 9495.0 19 7315.0 20 6673.0 21 7071.0 22 7453.0 23 8013.0 24 9640.0 25 11448.0 26 14401.0 27 17734.0 28 22157.0 29 27794.0 30 34013.0 31 41974.0 32 51412.0 33 64840.0 34 83674.0 35 110772.0 36 156008.0 37 196261.0 38 164930.0 39 31725.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.0604261077823 19.6410224450004 14.228759159180548 19.06979228803675 2 14.757738644916435 25.283843727147044 37.298960707594254 22.659456920342265 3 25.843454490608213 25.906010049821987 28.861426533701252 19.38910892586855 4 12.408545481719772 17.154661333599037 42.00449087945796 28.432302305223228 5 12.299031364985375 41.38850733415417 33.6874675283082 12.624993772552257 6 27.584010362731178 40.15051226481903 16.45741921019761 15.808058162252186 7 27.734383340331796 34.5045086721658 21.153750346957803 16.6073576405446 8 24.27850569722505 31.406798237810218 19.08712733173436 25.22756873323037 9 23.080433715046226 23.06335271552307 23.29020974043998 30.56600382899073 10-11 25.85611220858156 25.57147171899458 27.555370114928213 21.017045957495643 12-13 30.4914079013857 23.87091924245767 25.811125068501926 19.826547787654707 14-15 23.028473819299563 27.058001894992508 29.199565842092856 20.713958443615077 16-17 19.73940800102486 29.589095205221096 29.2631772794238 21.408319514330245 18-19 19.5896823645797 33.087630865366144 28.367180994541197 18.95550577551296 20-21 22.637277280732935 27.194717064062807 27.19427219273961 22.973733462464647 22-23 20.722944408463636 28.56552520514992 27.71574547887294 22.995784907513507 24-25 24.412306860156434 28.21256236344097 27.722106371924532 19.65302440447807 26-27 23.468999196609925 25.788198617955544 31.825012210995325 18.917789974439206 28-29 20.350366002575697 31.747937551575113 28.954751805545982 18.94694464030321 30-31 22.12657944044969 28.11248302896686 27.57928564183869 22.181651888744756 32-33 22.47221899717486 28.793578730523496 28.4114217387765 20.322780533525144 34-35 24.6609418538856 26.597292161182068 29.368165011263386 19.373600973668946 36-37 23.74243566141304 25.87077204524767 31.325326397706473 19.061465895632814 38-39 21.30679492791533 28.643203114712673 31.204735772574725 18.845266184797268 40-41 20.77879844635351 29.761779246425395 28.711630950680934 20.74779135654016 42-43 20.108473243325957 30.305518586262608 26.56878304995481 23.017225120456633 44-45 22.68672557239141 26.667354651369685 27.324706242393614 23.321213533845288 46-47 24.756828745251006 29.21066479486964 26.980165979644205 19.05234048023515 48-49 23.858508828741627 28.709245802700224 27.826816457543043 19.60542891101511 50-51 20.04143921655149 29.3610816542948 31.06184211462774 19.535637014525967 52-53 22.418055684058448 31.55180880667867 26.015385354466325 20.01475015479656 54-55 20.227728867600902 28.903853544663257 27.643773975673806 23.22464361206203 56-57 23.86893386997477 26.759030913820652 27.804778884511773 21.567256331692803 58-59 26.71748288889156 26.719217686393982 26.392630936065597 20.170668488648865 60-61 22.158524992204047 28.187321755137212 30.77531149339335 18.8788417592654 62-63 20.775262564106214 28.184312706643556 29.735318297808732 21.305106431441494 64-65 20.342528941265687 32.28222234386313 27.37890684279084 19.99634187208034 66-67 22.22392777929795 27.20066917161093 26.645621575445315 23.929781473645807 68-69 20.8821398180402 29.2215709501312 27.095177705053956 22.801111526774637 70-71 24.20513231114136 28.648954010257505 26.421749825408895 20.724163853192238 72-73 22.945564989644645 26.315948671596434 29.932005167002355 20.806481171756566 74-75 20.672457599977225 31.769013287594213 27.424034033891548 20.13449507853701 76-77 22.31717103062481 27.98890802593465 26.394503476695114 23.299417466745428 78-79 22.434158084650587 28.750435921342 27.22008512031429 21.595320873693126 80-81 23.9655319663789 27.515132698015044 27.523940088394173 20.99539524721188 82-83 22.918075256037067 26.854845310198066 29.540254222209573 20.686825211555295 84-85 20.81831332246792 28.787845089568492 28.8209840079142 21.572857580049394 86-87 21.11716809120611 29.175635371281018 26.512135830558766 23.19506070695411 88-89 20.844824457144483 29.341910011600845 25.717846797668443 24.09541873358623 90-91 22.716839730404892 25.356210010889136 27.063820663739175 24.8631295949668 92-93 23.55932444646886 28.585986819162034 26.88011095532607 20.974577779043038 94-95 22.598760736796685 28.84525223409887 27.3257091512426 21.230277877861848 96-97 19.434350767112065 29.244070678130534 29.87749248590036 21.444086068857043 98-99 21.75521161155729 30.92013050991835 25.1494480907253 22.17520978779906 100-101 19.577445178945478 28.641284790134904 26.3782940784765 25.402975952443125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 109.0 1 83.0 2 77.5 3 112.5 4 108.0 5 103.0 6 151.0 7 282.0 8 442.0 9 550.5 10 588.5 11 599.5 12 655.5 13 760.0 14 867.0 15 948.0 16 1074.0 17 1229.5 18 1346.0 19 1436.5 20 1552.0 21 1786.0 22 2129.0 23 2481.0 24 2983.0 25 3665.0 26 4639.5 27 5947.0 28 7799.5 29 10115.5 30 12598.5 31 15195.0 32 17805.0 33 20372.5 34 22748.5 35 24963.0 36 27263.0 37 29362.5 38 31420.0 39 33044.0 40 33990.5 41 34499.5 42 35463.0 43 37696.5 44 39039.5 45 40323.5 46 45348.5 47 71015.0 48 127222.0 49 122662.0 50 66607.5 51 41754.0 52 29718.5 53 22619.5 54 18137.5 55 14987.0 56 12222.5 57 9720.5 58 7719.0 59 6427.5 60 5255.5 61 4011.0 62 2960.0 63 2031.0 64 1308.0 65 838.5 66 515.0 67 280.0 68 153.5 69 91.0 70 43.0 71 22.0 72 13.5 73 8.0 74 5.0 75 3.0 76 2.0 77 1.0 78 2.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07704242493256563 2 0.001957197862028226 3 0.022685702491690805 4 0.0 5 0.0 6 0.0024909790971268334 7 0.0 8 0.0 9 0.0 10-11 5.782630046901577E-4 12-13 0.0 14-15 0.0036475051065071487 16-17 0.0 18-19 0.0 20-21 0.012054559559310212 22-23 0.0 24-25 0.0 26-27 0.006138484203633983 28-29 0.007873273217704455 30-31 0.007295010213014297 32-33 0.0036919868760986996 34-35 0.005337812350986072 36-37 0.017525817219070934 38-39 0.00889635391831012 40-41 0.010631142932380594 42-43 0.0 44-45 0.0 46-47 2.2240884795775298E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0024909790971268334 58-59 4.892994655070565E-4 60-61 0.006716747208324141 62-63 0.012454895485634169 64-65 0.04719515753663519 66-67 0.024776345662493683 68-69 0.0014678983965211697 70-71 8.89635391831012E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 7.561900830563602E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 8.89635391831012E-5 96-97 2.2240884795775298E-4 98-99 3.113723871408542E-4 100-101 0.001112044239788765 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1124056.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.3158821988091 #Duplication Level Percentage of deduplicated Percentage of total 1 83.55210896167182 41.20445963015869 2 10.1273663826819 9.9888001502504 3 2.4459001159915323 3.6186516597087577 4 1.0333882953178115 2.0384982175008535 5 0.5700778110337545 1.4056945086547796 6 0.36964978138309945 1.0937763044102682 7 0.26537664291207697 0.9161098282117192 8 0.1978027139737973 0.7803852272749214 9 0.1631332068645174 0.7240552211200046 >10 1.11481903273131 10.745611582249396 >50 0.0969885070147918 3.278083619338483 >100 0.05183052266473521 4.452100344680718 >500 0.004514853812012623 1.5742528326775702 >1k 0.005056636269454138 5.802274145514314 >5k 0.0012641590673635344 4.913617505750975 >10k+ 7.223766099220196E-4 7.463629222498214 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 48696 4.332168504060296 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13782 1.2260954970215006 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10765 0.9576924993060845 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10560 0.9394549737735486 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 9815 0.8731771370821383 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9522 0.8471108201014895 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8640 0.7686449785419943 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7944 0.7067263552705559 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6970 0.6200758681062154 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6652 0.5917854626459892 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5628 0.5006867985224935 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4613 0.4103888062516458 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4507 0.4009586710982371 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3888 0.3458902403438975 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3770 0.3353925427202915 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3726 0.33147814699623507 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3519 0.31306269438533313 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3267 0.2906438825111916 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3174 0.2823702733671632 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2979 0.2650223832264585 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2797 0.24883101909513403 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2797 0.24883101909513403 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2438 0.2168931085284007 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2235 0.19883351007423117 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2183 0.1942074060367099 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1976 0.17579195342580797 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1685 0.14990356352352552 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1648 0.14661191257375078 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1594 0.14180788145786333 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1532 0.13629214202851103 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1509 0.1342459806272997 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1442 0.12828542350203193 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1406 0.1250827360914403 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1183 0.10524386685360872 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.89635391831012E-5 0.0 10-11 0.0 0.0 0.0 1.3344530877465178E-4 0.0 12-13 0.0 0.0 0.0 1.779270783662024E-4 0.0 14-15 0.0 0.0 0.0 2.22408847957753E-4 0.0 16-17 0.0 0.0 0.0 3.558541567324048E-4 0.0 18-19 0.0 0.0 0.0 6.227447742817084E-4 0.0 20-21 0.0 0.0 0.0 9.785989310141133E-4 0.0 22-23 0.0 0.0 0.0 0.0021796067099859795 0.0 24-25 0.0 0.0 0.0 0.003647505106507149 0.0 26-27 0.0 0.0 0.0 0.00511540350302832 0.0 28-29 0.0 0.0 0.0 0.009385653383817177 0.0 30-31 0.0 0.0 0.0 0.02010575985538087 0.0 32-33 0.0 0.0 0.0 0.036652978143437695 0.0 34-35 0.0 0.0 0.0 0.053066751122719864 0.0 36-37 0.0 0.0 0.0 0.06703402677446675 0.0 38-39 0.0 0.0 0.0 0.07962236756887557 0.0 40-41 0.0 0.0 0.0 0.09817126548855218 0.0 42-43 0.0 0.0 0.0 0.1373152227291167 0.0 44-45 0.0 0.0 0.0 0.19122712747407603 0.0 46-47 0.0 0.0 0.0 0.24838620139921855 0.0 48-49 0.0 0.0 0.0 0.3645725835723487 0.0 50-51 0.0 0.0 0.0 0.46701409894168977 0.0 52-53 0.0 0.0 0.0 0.5626499035635235 0.0 54-55 0.0 0.0 0.0 0.7865266499177976 0.0 56-57 0.0 0.0 0.0 1.0340232159251852 0.0 58-59 0.0 0.0 0.0 1.3058513098991509 0.0 60-61 0.0 0.0 0.0 1.5647352089219755 0.0 62-63 0.0 0.0 0.0 1.9657383617898043 0.0 64-65 0.0 0.0 0.0 2.181964243774331 0.0 66-67 0.0 0.0 0.0 2.6718864540556697 0.0 68-69 0.0 0.0 0.0 3.2730575700854763 0.0 70-71 0.0 0.0 0.0 3.4741596504088763 0.0 72-73 0.0 0.0 0.0 3.8416235490046757 0.0 74-75 0.0 0.0 0.0 4.3861248905748464 0.0 76-77 0.0 0.0 0.0 4.568144291743472 0.0 78-79 0.0 0.0 0.0 4.747272377888646 0.0 80-81 0.0 0.0 0.0 5.152501298867672 0.0 82-83 0.0 0.0 0.0 5.77235475812593 0.0 84-85 0.0 0.0 0.0 6.743169379461522 0.0 86-87 0.0 0.0 0.0 8.02513397909001 0.0 88-89 0.0 0.0 0.0 9.144028411395873 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 860 0.0 40.32821 2 ACCTGGG 885 0.0 38.65216 3 TATAACG 155 3.6379788E-11 33.716736 2 AGTACGC 500 0.0 33.25696 2 ATAACGC 215 0.0 33.146473 3 GAGTACG 585 0.0 31.690222 1 GTATCGC 60 0.0039528897 31.663437 6 TACGCAG 530 0.0 30.468588 4 GTACGCA 545 0.0 29.639233 3 TAACGCA 245 0.0 27.140085 4 TCACGCA 90 8.952756E-4 26.386196 4 CTACGTA 90 8.952756E-4 26.386196 9 GTACCTG 1405 0.0 26.05139 1 CCTGGGG 665 0.0 25.71166 4 GTACCCG 145 2.320905E-5 22.94809 1 GTATAAC 290 3.6379788E-12 22.94809 1 CGCAGTG 715 0.0 21.92084 6 TACACAG 495 0.0 21.108955 5 CCGTGCG 115 0.0037254307 20.650066 9 ACGCAGT 765 0.0 20.488104 5 >>END_MODULE