##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003848_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2126947 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.66738757477267 31.0 31.0 34.0 30.0 34.0 2 31.86217475094584 31.0 31.0 34.0 30.0 34.0 3 31.918557444073596 31.0 31.0 34.0 30.0 34.0 4 35.59057042794202 37.0 35.0 37.0 33.0 37.0 5 35.5101001576438 37.0 35.0 37.0 33.0 37.0 6 35.577280016850445 37.0 35.0 37.0 33.0 37.0 7 35.52766430005073 37.0 35.0 37.0 33.0 37.0 8 35.539934939610625 37.0 35.0 37.0 33.0 37.0 9 37.08630257359492 39.0 37.0 39.0 33.0 39.0 10-11 36.987290703529524 39.0 37.0 39.0 33.0 39.0 12-13 36.95967741556325 39.0 37.0 39.0 33.0 39.0 14-15 37.930436912626405 40.0 37.0 41.0 33.0 41.0 16-17 38.025595137067356 40.0 37.0 41.0 33.0 41.0 18-19 38.10487285296719 40.0 37.0 41.0 33.0 41.0 20-21 38.02759965339992 40.0 37.0 41.0 33.0 41.0 22-23 37.92735432523706 40.0 37.0 41.0 33.0 41.0 24-25 37.76720294393796 39.5 37.0 41.0 32.5 41.0 26-27 37.463232276121595 39.0 36.5 41.0 31.5 41.0 28-29 37.34788431493592 39.0 36.0 41.0 32.0 41.0 30-31 37.20377940776145 39.0 36.0 40.0 31.0 41.0 32-33 36.95706498563434 39.0 36.0 40.0 31.0 41.0 34-35 36.88695063863838 39.0 35.0 40.0 30.5 41.0 36-37 36.6511767806156 38.5 35.0 40.0 30.0 41.0 38-39 36.40358974624192 38.0 35.0 40.0 30.0 41.0 40-41 36.69366067889797 39.0 35.0 40.0 30.0 41.0 42-43 36.59517867629047 39.0 35.0 40.5 30.0 41.0 44-45 36.44920254242348 39.0 35.0 41.0 30.0 41.0 46-47 36.18603801599194 39.0 35.0 40.0 29.5 41.0 48-49 35.89341624403429 39.0 35.0 40.0 28.0 41.0 50-51 35.58424304883948 38.5 35.0 40.0 28.0 41.0 52-53 35.46675704660248 38.0 34.5 40.0 27.5 41.0 54-55 35.294408370307295 38.0 34.0 40.0 27.0 41.0 56-57 34.98262932738803 38.0 34.0 40.0 26.5 41.0 58-59 34.70653641110944 38.0 34.0 40.0 26.0 41.0 60-61 34.096269206519956 37.5 33.0 40.0 23.5 41.0 62-63 33.96507952478365 37.0 33.0 40.0 24.5 41.0 64-65 33.54338895139371 36.5 32.0 39.5 22.5 41.0 66-67 33.11806570638572 36.0 32.0 39.0 22.0 40.0 68-69 32.76345719945067 36.0 32.0 39.0 22.0 40.0 70-71 32.21859877091437 35.0 31.0 37.5 21.5 40.0 72-73 31.750905640808163 35.0 31.0 37.0 20.5 39.5 74-75 31.412912968682342 35.0 31.0 37.0 20.0 39.0 76-77 30.46992637804327 34.0 29.5 36.0 19.0 38.5 78-79 30.835540330812194 34.0 30.0 36.0 18.5 38.5 80-81 30.55458669162889 34.0 30.0 35.5 18.0 37.0 82-83 30.2152413764894 34.0 30.0 35.0 14.5 37.0 84-85 29.87266937069894 34.0 29.5 35.0 10.5 36.5 86-87 29.68558948577468 34.0 30.0 35.0 7.0 36.0 88-89 29.340982403416728 34.0 29.0 35.0 4.5 36.0 90-91 28.96116264298076 34.0 29.0 35.0 2.0 35.5 92-93 28.749459671538595 34.0 29.0 35.0 2.0 35.0 94-95 28.36104026099381 34.0 29.0 35.0 2.0 35.0 96-97 27.879452567459367 34.0 28.5 35.0 2.0 35.0 98-99 27.589127749774676 34.0 28.5 35.0 2.0 35.0 100-101 26.53440800358448 33.0 25.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06488104525831773 1101 2 0.15760781320134853 1101 3 0.12850087660218534 1101 4 0.07638021569381692 1101 5 0.10774115094844916 1101 6 0.1828719984947753 1101 7 -0.011283885707918273 1101 8 0.08705193015154578 1101 9 0.21636494591563604 1101 10-11 0.21975171059895615 1101 12-13 0.24025103886810228 1101 14-15 0.16029172204120812 1101 16-17 0.24641389040596806 1101 18-19 0.13026342687790304 1101 20-21 0.06545975190048381 1101 22-23 0.1699240685968988 1101 24-25 0.10595810338568867 1101 26-27 0.2533246549077717 1101 28-29 0.18254779684264122 1101 30-31 0.20960404860169746 1101 32-33 0.202819805329284 1101 34-35 0.2864959107072522 1101 36-37 0.26080867375409156 1101 38-39 0.17923760425699697 1101 40-41 0.2791204164483432 1101 42-43 0.06662039953226184 1101 44-45 0.1082834067959979 1101 46-47 0.03052355017151598 1101 48-49 0.2043153114163374 1101 50-51 0.2863136746396222 1101 52-53 0.41400732333300283 1101 54-55 0.4234268958704277 1101 56-57 0.5748364616788848 1101 58-59 0.3698493633866775 1101 60-61 0.3691574231238164 1101 62-63 0.20057733897254337 1101 64-65 0.15169069745623887 1101 66-67 0.06602206332426164 1101 68-69 -0.010684931327354263 1101 70-71 0.257519097490686 1101 72-73 0.2184661998813695 1101 74-75 -0.02294004610478595 1101 76-77 0.07429084960227073 1101 78-79 0.23242507028853865 1101 80-81 0.07385503612458422 1101 82-83 0.21599156360045058 1101 84-85 0.12893656974448575 1101 86-87 -0.06605914865979301 1101 88-89 0.015780546117422745 1101 90-91 0.0652312074404442 1101 92-93 0.07483602227686603 1101 94-95 0.17618401859098753 1101 96-97 0.43326330141072233 1101 98-99 0.21222618126081016 1101 100-101 0.39436474647070696 1102 1 -0.025859381884870913 1102 2 -0.011438363660630557 1102 3 0.03301824896090011 1102 4 0.014484962314583072 1102 5 -0.03565374790109388 1102 6 0.060650989547468726 1102 7 0.008772826932897715 1102 8 0.0026409631241222087 1102 9 0.0490279392523334 1102 10-11 0.09145697118181317 1102 12-13 0.14477497098489778 1102 14-15 0.11017315189085508 1102 16-17 0.10378264831678763 1102 18-19 -0.010278179043723412 1102 20-21 -0.048519375151123256 1102 22-23 -0.03299528614089553 1102 24-25 -0.015292666881293826 1102 26-27 -0.028750190519097885 1102 28-29 0.12238734717828947 1102 30-31 -0.020290141491209113 1102 32-33 0.06255986532786295 1102 34-35 0.027026597154652166 1102 36-37 0.06972544374536227 1102 38-39 0.12338388807330603 1102 40-41 0.18656969791262412 1102 42-43 0.3126533101413358 1102 44-45 0.3787084246233974 1102 46-47 0.1998335302278278 1102 48-49 0.28103894840457855 1102 50-51 0.4301211318129887 1102 52-53 0.4470913118516151 1102 54-55 0.39236109261593555 1102 56-57 0.5863965719716333 1102 58-59 0.17999422101065932 1102 60-61 0.30495767626992887 1102 62-63 -0.02754258793762432 1102 64-65 0.03734382027449357 1102 66-67 0.019150169748165524 1102 68-69 -0.06627968844575349 1102 70-71 0.0977352990435918 1102 72-73 0.11816598498369757 1102 74-75 0.18991804856604588 1102 76-77 0.15007512923284594 1102 78-79 0.08585888162303235 1102 80-81 0.14475340609534726 1102 82-83 0.12426323688978158 1102 84-85 0.1472005509605694 1102 86-87 0.020024354840398217 1102 88-89 0.04324100540948095 1102 90-91 0.08840743826803887 1102 92-93 0.280080659876468 1102 94-95 0.2272518361958049 1102 96-97 0.2977954160688334 1102 98-99 0.18290809939865937 1102 100-101 0.16358175961706678 1103 1 0.03295254358224753 1103 2 -0.09901246011907006 1103 3 0.04032326998192559 1103 4 -0.05208791667336499 1103 5 -0.0077366959265674495 1103 6 -0.10744470795102501 1103 7 -0.08523661217704159 1103 8 0.017855482138109835 1103 9 -0.05447210521197832 1103 10-11 -0.058841613110146795 1103 12-13 -0.06896428355556594 1103 14-15 -0.002154197344928832 1103 16-17 0.026746989919161024 1103 18-19 0.04141737688198077 1103 20-21 0.01902359826951283 1103 22-23 0.044722092354184895 1103 24-25 -0.0811688722949242 1103 26-27 -0.21748158705698017 1103 28-29 0.0297730377506511 1103 30-31 0.03790849336528623 1103 32-33 -0.07068403102929466 1103 34-35 -0.07689907673714913 1103 36-37 -0.23197234034743275 1103 38-39 -0.25300958286237574 1103 40-41 -0.06621110412057618 1103 42-43 -0.1391357891899574 1103 44-45 -0.2307578590494046 1103 46-47 -0.09220579325707234 1103 48-49 -0.04364183447628989 1103 50-51 0.043708633320711954 1103 52-53 0.01995316767117572 1103 54-55 -0.1389808007081328 1103 56-57 -0.03809340058329269 1103 58-59 -0.13124992212837583 1103 60-61 -0.2061040344513998 1103 62-63 -0.29664882357707967 1103 64-65 -0.14651333698439828 1103 66-67 -0.2969058239915725 1103 68-69 -0.3732621897088748 1103 70-71 -0.2554927604922419 1103 72-73 -0.16354523941293309 1103 74-75 -0.1608443707916365 1103 76-77 -0.19592049801401146 1103 78-79 -0.3475719034859992 1103 80-81 -0.271638733881872 1103 82-83 -0.24816518596602322 1103 84-85 -0.2876420008116334 1103 86-87 -0.29155619201320704 1103 88-89 -0.27683499786483523 1103 90-91 -0.34341678886002214 1103 92-93 -0.32395810587345153 1103 94-95 -0.5214793464380421 1103 96-97 -0.18942421366723394 1103 98-99 -0.34873494308525466 1103 100-101 -0.3427523747340757 1104 1 0.04810469566150388 1104 2 0.0046876648282108135 1104 3 -0.060113739596051374 1104 4 -0.003079018398132405 1104 5 0.02117761660583284 1104 6 -0.051328359948279 1104 7 0.01490968922753666 1104 8 0.026489495152283382 1104 9 -0.02837330035215757 1104 10-11 0.010500673955988304 1104 12-13 -0.03808596397116304 1104 14-15 -0.05120345530885828 1104 16-17 -0.15372466386300232 1104 18-19 -0.08661865356402387 1104 20-21 -0.05095358913187198 1104 22-23 -0.019635427780762882 1104 24-25 0.05587171060554397 1104 26-27 0.05727217443639887 1104 28-29 -0.042405084850834385 1104 30-31 -0.07187913572898452 1104 32-33 0.13377104705831044 1104 34-35 0.14770180146598477 1104 36-37 0.14994152595878774 1104 38-39 0.13279095106966565 1104 40-41 0.08165041458391897 1104 42-43 0.1333097730895787 1104 44-45 0.1607061556008702 1104 46-47 0.1701939563352397 1104 48-49 0.13949799931284446 1104 50-51 0.24075558693390775 1104 52-53 0.2687251575340852 1104 54-55 0.16650888665130736 1104 56-57 0.09668032958150263 1104 58-59 0.02489046406549278 1104 60-61 0.17893351387414214 1104 62-63 0.10751951323073428 1104 64-65 -0.016489024465805358 1104 66-67 0.07201143312055791 1104 68-69 0.03445592480488102 1104 70-71 0.24505293407629125 1104 72-73 0.3575985995572353 1104 74-75 0.24049799837537833 1104 76-77 0.187732584706378 1104 78-79 0.20413477847985462 1104 80-81 0.25474347690937904 1104 82-83 0.2975066864097329 1104 84-85 0.22039747155598732 1104 86-87 0.3320540877277871 1104 88-89 0.4573315212768101 1104 90-91 0.385477708940126 1104 92-93 0.17798192219478892 1104 94-95 0.2141504827862022 1104 96-97 0.42255576555887586 1104 98-99 0.5106970964289488 1104 100-101 0.45296089345930923 1105 1 0.1130063102985055 1105 2 0.1265888149890877 1105 3 0.1649188517511 1105 4 0.24298966037141412 1105 5 0.194381896968153 1105 6 0.2043643582074779 1105 7 0.1469083323502005 1105 8 0.14404064363523617 1105 9 0.22629085861367315 1105 10-11 0.23416879988566563 1105 12-13 0.14193294852200466 1105 14-15 0.3275171897678959 1105 16-17 0.24427174045482047 1105 18-19 0.29812137126076266 1105 20-21 0.23695866014880806 1105 22-23 0.3469910968626593 1105 24-25 0.2950127910073732 1105 26-27 0.40652177723770677 1105 28-29 0.513496636874315 1105 30-31 0.4680679486199253 1105 32-33 0.46852976421501324 1105 34-35 0.5649942711601526 1105 36-37 0.37975300593618755 1105 38-39 0.5248143591274328 1105 40-41 0.4173929105584975 1105 42-43 0.5358950433610303 1105 44-45 0.5381774389849525 1105 46-47 0.2984600111673572 1105 48-49 0.5198958242906286 1105 50-51 0.5606026539477895 1105 52-53 0.6081998143016207 1105 54-55 0.5873166689883433 1105 56-57 0.5409124146005126 1105 58-59 0.3733965416917826 1105 60-61 0.5533850611909656 1105 62-63 0.6618012328568668 1105 64-65 0.5317614523543099 1105 66-67 0.5115181735818553 1105 68-69 0.5028224928692353 1105 70-71 0.533014345579268 1105 72-73 0.42996645951929224 1105 74-75 0.3021332108384698 1105 76-77 0.4365143769002273 1105 78-79 0.5167289214636881 1105 80-81 0.6076842007354664 1105 82-83 0.48482266205861535 1105 84-85 0.3744258133633913 1105 86-87 0.47937189306633954 1105 88-89 0.579848588583527 1105 90-91 0.7149940232098473 1105 92-93 0.5079529870207367 1105 94-95 0.8371789440791844 1105 96-97 0.7335720149558362 1105 98-99 0.4952232869616324 1105 100-101 0.736653741574333 1106 1 -0.04455808533638006 1106 2 0.016938355301899577 1106 3 0.013476162222350041 1106 4 0.008704762093685758 1106 5 0.006721428981762756 1106 6 -0.03214299979268276 1106 7 0.027935110549577757 1106 8 0.0895842797429296 1106 9 0.028019026663805846 1106 10-11 -0.010359161283204799 1106 12-13 -0.024946300921079967 1106 14-15 -0.028240971546615867 1106 16-17 -0.08802665933350795 1106 18-19 0.05989361794475201 1106 20-21 -0.006938105286558027 1106 22-23 -0.08906166915747349 1106 24-25 -0.017730516476348157 1106 26-27 0.16720233887198788 1106 28-29 -0.03973619922244609 1106 30-31 -0.06600216008763482 1106 32-33 -0.04821053630510619 1106 34-35 0.009595463235484658 1106 36-37 0.09027254317831535 1106 38-39 0.33650021322552703 1106 40-41 0.13757831738544013 1106 42-43 0.054617730051830904 1106 44-45 0.06566749881015 1106 46-47 0.04377134645758218 1106 48-49 0.05504891891214214 1106 50-51 0.11434851310267646 1106 52-53 0.17199317525678026 1106 54-55 0.0186956266149636 1106 56-57 0.015600189061068193 1106 58-59 0.003768794657233343 1106 60-61 0.19537054741729776 1106 62-63 0.013519492930306853 1106 64-65 0.049297328381008754 1106 66-67 -0.12340655177964521 1106 68-69 -0.05323801425196706 1106 70-71 -0.04411784083892201 1106 72-73 0.023750712366336302 1106 74-75 0.12132585421660735 1106 76-77 0.13844820607676667 1106 78-79 0.11000964121888046 1106 80-81 0.07255505698122988 1106 82-83 0.14884836902156096 1106 84-85 0.24452681597832893 1106 86-87 0.3527472729756731 1106 88-89 0.16566296439116002 1106 90-91 0.23011786614005914 1106 92-93 0.070855081975985 1106 94-95 -0.006081157594508113 1106 96-97 0.1871242787985885 1106 98-99 -0.002526461271820324 1106 100-101 0.03012066802506297 1107 1 -0.05611836934343373 1107 2 0.018110469336672708 1107 3 -0.053107789039305686 1107 4 0.022163600868388755 1107 5 -0.060974118334748084 1107 6 0.030866791071574085 1107 7 -0.16650375954149155 1107 8 -0.016014420807806573 1107 9 -0.04320478637387737 1107 10-11 -0.020019765653096044 1107 12-13 0.05076461661247578 1107 14-15 0.09842484771834137 1107 16-17 -0.06561385921833107 1107 18-19 -0.010188902482880735 1107 20-21 0.11844637511371303 1107 22-23 0.020985102916959875 1107 24-25 0.039363810581718894 1107 26-27 0.11061319071317399 1107 28-29 -0.048375647046498216 1107 30-31 -0.08076123553488657 1107 32-33 -0.09480769353486806 1107 34-35 -0.11077412389240493 1107 36-37 -0.1501718027775567 1107 38-39 0.02705907089803361 1107 40-41 0.05614091412422084 1107 42-43 0.05465665053705493 1107 44-45 0.035702324770340965 1107 46-47 -0.12541037846232683 1107 48-49 -0.1865865513733027 1107 50-51 -0.055436141877343914 1107 52-53 0.014121563363758582 1107 54-55 -0.2785690281850677 1107 56-57 -0.20107352678789425 1107 58-59 -0.12199645407391557 1107 60-61 -0.18255748793060178 1107 62-63 -0.18072792436928609 1107 64-65 -0.20649417480462517 1107 66-67 -0.25463135470250364 1107 68-69 -0.1386089737206717 1107 70-71 -0.031091739366686966 1107 72-73 0.01545052518805079 1107 74-75 -0.04319362402391391 1107 76-77 -0.08034318912138616 1107 78-79 -0.11502320497096008 1107 80-81 -0.11434842471489048 1107 82-83 -0.002511935683092048 1107 84-85 -0.15545361129210633 1107 86-87 -0.027070205696801963 1107 88-89 -0.17049765347716672 1107 90-91 0.0721828116063854 1107 92-93 -0.13610808529661256 1107 94-95 -0.06743514053248845 1107 96-97 0.08565294947279156 1107 98-99 -0.017770018019241718 1107 100-101 0.1698031398583275 1108 1 -0.10892709837545311 1108 2 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-1.5731325402823053 2316 88-89 -1.5823335943121357 2316 90-91 -1.7077011848078563 2316 92-93 -1.5857052209578484 2316 94-95 -1.79445426458269 2316 96-97 -1.760251174150529 2316 98-99 -1.9319246965815218 2316 100-101 -2.1222222806146505 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 20.0 5 33.0 6 102.0 7 277.0 8 638.0 9 1232.0 10 2124.0 11 3693.0 12 5677.0 13 8258.0 14 9127.0 15 11614.0 16 14552.0 17 18459.0 18 16905.0 19 12974.0 20 12150.0 21 12398.0 22 13498.0 23 14886.0 24 16952.0 25 20909.0 26 25831.0 27 31894.0 28 40284.0 29 50749.0 30 62368.0 31 77134.0 32 95530.0 33 119612.0 34 155851.0 35 207443.0 36 293203.0 37 379511.0 38 328112.0 39 62902.0 40 35.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.41770886595766 19.129646973266983 13.593333185942905 18.859310974832457 2 14.275545040319985 25.68381290549782 36.90231754684486 23.138324507337337 3 26.661846958103812 25.701636835116577 29.056492660135756 18.58002354664385 4 12.001474413795925 16.613719100664003 42.20307323125588 29.181733254284193 5 11.566578762893482 41.427830594744485 33.4356709405547 13.569919701807331 6 26.19565248054223 39.83611937295067 17.311560316966272 16.65666782954083 7 27.059220121244266 34.23951524862409 21.782264335812947 16.91900029431871 8 23.716528902694805 29.93088215174144 19.31392742743472 27.038661518129036 9 22.539254621765377 23.184639767704603 24.58340522824499 29.692700382285032 10-11 25.305232889574064 24.980376752252003 27.940213682416747 21.774176675757182 12-13 31.168853760813032 23.06867543008829 25.849304190466427 19.913166618632246 14-15 23.303419631237258 26.74992101165266 30.188523557334257 19.758135799775822 16-17 19.457842626073898 29.235730838615158 30.111657695278726 21.194768840032214 18-19 18.725830968049507 33.62439214517334 28.54796099761771 19.101815889159436 20-21 22.2542671744957 27.60532750270372 26.54669205811821 23.59371326468237 22-23 19.99969909922532 28.382700650274785 27.66112648787205 23.95647376262784 24-25 24.19317453608388 28.120940484177552 27.575510814326826 20.110374165411738 26-27 23.607910845356813 24.728315363650104 32.41682894276855 19.24694484822453 28-29 19.57750972329031 31.91832412629098 29.47635176834224 19.027814382076468 30-31 21.52946611511375 28.186846191733995 27.45155985324837 22.83212783990389 32-33 22.079415083693814 28.45302802332142 28.769865525672373 20.697691367312395 34-35 24.86668172862012 25.99903186590567 29.33580311754225 19.798483287931965 36-37 23.816963902583083 25.395983417896794 31.689277663701947 19.097775015818176 38-39 21.36452227966606 28.329160230684415 31.806283399515216 18.500034090134314 40-41 20.84763308496809 29.797433932737544 28.94898265549944 20.405950326794926 42-43 19.40584321094978 30.67177978576805 26.470452719320225 23.45192428396194 44-45 22.231042898577165 26.48227717945017 26.88553123326533 24.401148688707337 46-47 24.573302619506414 29.398224167838734 26.609014378123756 19.4194588345311 48-49 23.858939597460584 28.3657514738261 27.97890591538012 19.796403013333194 50-51 19.474345152935168 29.362767384424714 31.500620372769045 19.66226708987107 52-53 21.73098342365842 31.67789324322609 26.55066158207045 20.04046175104504 54-55 19.827221834864712 28.554496186317756 27.948956885150405 23.66932509366712 56-57 23.34747113434343 26.846118475430693 27.793120763243785 22.01328962698209 58-59 26.92197024370635 26.245036895641398 26.259987916941906 20.573004943710345 60-61 22.566594301744328 28.028500212298653 31.00086941352439 18.404036072432632 62-63 21.193311092777165 28.07619032392385 29.978056475714354 20.752442107584635 64-65 20.172581822560375 32.64389030621844 27.515493418824644 19.66803445239654 66-67 21.9595833811438 27.705892256208937 26.375906909725682 23.95861745292158 68-69 20.369813572227685 29.595006275537344 26.961937026469627 23.07324312576534 70-71 24.107484981938835 28.7993449741624 26.061090609556736 21.03207943434203 72-73 23.133063494294877 25.92034498273817 30.13504802893537 20.811543494031586 74-75 20.103674421600537 32.312652830559486 27.592577530140623 19.99109521769936 76-77 22.18868171139196 27.83160558302581 26.552612735531255 23.427099970050968 78-79 22.17913751494513 28.680028228253924 27.729228796016077 21.411605460784873 80-81 24.286030634519808 27.730427697540183 27.388623223803883 20.594918444136127 82-83 22.78098608004807 26.7253485864951 29.8826439963008 20.61102133715603 84-85 20.82854947892424 28.977533985348007 29.60881470427552 20.58510183145223 86-87 21.41345241554275 29.80057209109451 26.70962051478795 22.076354978574795 88-89 20.559515587365365 29.828975522192135 25.622476723679526 23.98903216676297 90-91 22.394540155443458 25.705765117795604 27.00805426745471 24.891640459306227 92-93 23.566736735800188 29.089793962896117 26.93750714051643 20.40596216078727 94-95 22.53369153113575 28.39395395459401 27.62364688492034 21.448707629349904 96-97 18.508854991405478 29.219920110431158 30.767992078746143 21.503232819417228 98-99 21.0994174482489 31.04842624517763 26.091599862807314 21.76055644376616 100-101 19.192345187779438 29.155656044987044 26.86610496334259 24.785893803890925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 379.0 1 265.0 2 341.0 3 560.0 4 456.0 5 330.5 6 452.5 7 732.5 8 1046.0 9 1233.0 10 1266.0 11 1295.0 12 1418.5 13 1594.0 14 1812.5 15 2092.5 16 2308.5 17 2515.5 18 2805.0 19 3019.0 20 3255.5 21 3653.0 22 4307.5 23 5186.5 24 6239.5 25 7827.5 26 9884.0 27 12669.0 28 16713.5 29 21576.5 30 26183.5 31 30544.0 32 34936.0 33 38795.0 34 42589.5 35 46527.5 36 49899.5 37 53188.0 38 56638.0 39 58697.5 40 59811.0 41 60579.0 42 62262.5 43 65105.0 44 66131.0 45 67997.5 46 77546.5 47 134947.0 48 268920.0 49 262780.5 50 131026.5 51 73615.5 52 50425.0 53 39200.5 54 32520.0 55 27179.5 56 22263.0 57 17536.0 58 13711.0 59 11104.0 60 8664.5 61 6335.0 62 4553.5 63 3067.5 64 1869.5 65 1131.5 66 691.5 67 403.0 68 217.0 69 120.0 70 72.5 71 41.0 72 25.0 73 14.0 74 10.0 75 8.0 76 7.0 77 4.5 78 2.5 79 1.0 80 0.5 81 1.5 82 1.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05049491125072698 2 0.004043354159741639 3 0.044853021725506086 4 0.0 5 0.0 6 0.0015515196194357452 7 4.701574604350743E-5 8 0.0 9 0.0 10-11 2.5858660323929087E-4 12-13 0.0 14-15 0.002397803048218879 16-17 0.0 18-19 0.0 20-21 0.011612889272746335 22-23 0.0 24-25 0.0 26-27 0.008016184700418016 28-29 0.010508019240723912 30-31 0.010555034986767419 32-33 0.006911314668395593 34-35 0.008251263430635553 36-37 0.0205693888940345 38-39 0.010907653082093724 40-41 0.014457341908378536 42-43 0.0 44-45 0.0 46-47 2.3507873021753715E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0011753936510876858 58-59 9.403149208701486E-5 60-61 0.01062555860583268 62-63 0.01652603473429286 64-65 0.04151490375641706 66-67 0.02327279429153618 68-69 0.0017630904766315287 70-71 3.7612596834805945E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 4.701574604350743E-5 86-87 6.112046985655966E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.410472381305223E-4 96-97 5.171732064785817E-4 98-99 5.406810795003355E-4 100-101 0.0011048700320224246 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2126947.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.00940895763751 #Duplication Level Percentage of deduplicated Percentage of total 1 82.14227248031135 39.43601952216961 2 11.613444405802593 11.151092037699286 3 2.671197071006783 3.8472777776522435 4 1.0451536404260737 2.0070883418711603 5 0.5494269127125913 1.3188830672375502 6 0.36259945192825915 1.044491122526341 7 0.2511961864651364 0.8441846311222592 8 0.18527448027841933 0.7115934634480304 9 0.1424451908746906 0.6154838480476581 >10 0.9002481656554654 8.230249478028597 >50 0.08359577153204696 2.755727754666308 >100 0.044109056745950136 3.996890903011676 >500 0.002652217706925804 0.8728331982078998 >1k 0.00412567198855125 4.649388337201234 >5k 8.840725689752681E-4 2.8290208501240275 >10k+ 0.0013752239961837504 15.689775666986117 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 91935 4.322392612509856 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 32907 1.547147155053699 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 27687 1.3017249607065902 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 26812 1.2605861829185212 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 22431 1.0546101995019153 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 18662 0.8774078526639356 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 17869 0.8401243660514343 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 15738 0.73993381123272 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 15286 0.7186826940210546 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 13923 0.654600232163754 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 13829 0.6501807520356643 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 12428 0.5843116918287103 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 12197 0.5734510544926601 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10990 0.5167030490181467 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 8796 0.4135505021986913 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7254 0.34105222179960293 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7185 0.3378081353226009 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 7091 0.33338865519451116 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 6602 0.3103979553792361 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6225 0.29267301912083377 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 6200 0.29149762546974606 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5537 0.2603261858429007 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5098 0.23968627332980086 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4672 0.2196575655152667 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 4290 0.20169755052664687 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4281 0.2012744088122553 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 4261 0.20033409389138518 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3881 0.18246811039485236 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3834 0.1802583703308075 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3368 0.15834903267453304 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3253 0.15294222187952966 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3162 0.1486637889895705 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3121 0.1467361434017867 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3038 0.14283383648017559 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2859 0.13441801793838776 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2852 0.1340889077160832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2782 0.13079780549303766 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2574 0.12101853031598812 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 2463 0.11579978250515881 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2408 0.1132139164727659 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2361 0.11100417640872104 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2319 0.10902951507489372 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2283 0.10733694821732746 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2176 0.10230626339067217 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.403149208701486E-5 0.0 10-11 0.0 0.0 0.0 1.64555111152276E-4 0.0 12-13 0.0 0.0 0.0 2.820944762610446E-4 0.0 14-15 0.0 0.0 0.0 3.056023492827983E-4 0.0 16-17 0.0 0.0 0.0 5.641889525220892E-4 0.0 18-19 0.0 0.0 0.0 9.638227938919023E-4 0.0 20-21 0.0 0.0 0.0 0.0013399487622399618 0.0 22-23 0.0 0.0 0.0 0.0022097400640448493 0.0 24-25 0.0 0.0 0.0 0.0035026730802413036 0.0 26-27 0.0 0.0 0.0 0.005618381652199138 0.0 28-29 0.0 0.0 0.0 0.01133079479648529 0.0 30-31 0.0 0.0 0.0 0.02708106972106028 0.0 32-33 0.0 0.0 0.0 0.05782936763351414 0.0 34-35 0.0 0.0 0.0 0.0848164058624874 0.0 36-37 0.0 0.0 0.0 0.09830992497697405 0.0 38-39 0.0 0.0 0.0 0.10778359780474078 0.0 40-41 0.0 0.0 0.0 0.12273460504657616 0.0 42-43 0.0 0.0 0.0 0.1370744075898459 0.0 44-45 0.0 0.0 0.0 0.15305976124463844 0.0 46-47 0.0 0.0 0.0 0.17200710690017193 0.0 48-49 0.0 0.0 0.0 0.19053131084131386 0.0 50-51 0.0 0.0 0.0 0.21770641205446117 0.0 52-53 0.0 0.0 0.0 0.28646694064309075 0.0 54-55 0.0 0.0 0.0 0.3628205122177468 0.0 56-57 0.0 0.0 0.0 0.46256441744904786 0.0 58-59 0.0 0.0 0.0 0.6305751859355216 0.0 60-61 0.0 0.0 0.0 0.8335186537323215 0.0 62-63 0.0 0.0 0.0 0.9182645359757436 0.0 64-65 0.0 0.0 0.0 1.0128837248883023 0.0 66-67 0.0 0.0 0.0 1.3715668514542205 0.0 68-69 2.3507873021753715E-5 0.0 0.0 1.7181434234139354 0.0 70-71 4.701574604350743E-5 0.0 0.0 1.920640241623322 0.0 72-73 4.701574604350743E-5 0.0 0.0 2.039519555494331 0.0 74-75 4.701574604350743E-5 0.0 0.0 2.509418429326166 0.0 76-77 4.701574604350743E-5 0.0 0.0 2.629402613229197 0.0 78-79 4.701574604350743E-5 0.0 0.0 2.7196023220136656 0.0 80-81 4.701574604350743E-5 0.0 0.0 2.885661937039334 0.0 82-83 4.701574604350743E-5 0.0 0.0 3.2776792275500988 0.0 84-85 4.701574604350743E-5 0.0 0.0 3.664477770250034 0.0 86-87 4.701574604350743E-5 0.0 0.0 4.419785730438981 0.0 88-89 4.701574604350743E-5 0.0 0.0 5.442213651774115 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 315 0.0 52.79133 2 TACCTGG 2805 0.0 52.678406 2 ACCTGGG 3045 0.0 46.34085 3 GTACCTG 3460 0.0 43.695023 1 GTACCGG 120 8.54925E-11 39.618652 1 GTACCCG 370 0.0 38.547882 1 ATAACGC 455 0.0 37.591183 3 GTATAAC 575 0.0 33.899788 1 TAACGCA 500 0.0 33.244465 4 AGTACGC 1155 0.0 32.08616 2 CCTGGGG 1740 0.0 31.661392 4 TACGCAG 1220 0.0 30.753075 4 TACCCGG 435 0.0 30.582561 2 GTACGCA 1200 0.0 30.486275 3 GAGTACG 1270 0.0 30.32231 1 TACACGG 240 0.0 29.69512 2 GTACACG 285 0.0 28.358616 1 ACGCAGT 1400 0.0 25.44219 5 TCACGCA 380 0.0 23.746044 4 CGCAGTG 1555 0.0 23.211567 6 >>END_MODULE