##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003847_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2288399 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.689494270885454 31.0 31.0 34.0 30.0 34.0 2 31.87919021114762 31.0 31.0 34.0 30.0 34.0 3 31.937903311441755 31.0 31.0 34.0 30.0 34.0 4 35.60948068933783 37.0 35.0 37.0 33.0 37.0 5 35.52727561933037 37.0 35.0 37.0 33.0 37.0 6 35.598843995299774 37.0 35.0 37.0 33.0 37.0 7 35.55153406377122 37.0 35.0 37.0 33.0 37.0 8 35.5620156275195 37.0 35.0 37.0 33.0 37.0 9 37.113471033679005 39.0 37.0 39.0 33.0 39.0 10-11 37.01719280597483 39.0 37.0 39.0 33.0 39.0 12-13 36.9955685175531 39.0 37.0 39.0 33.0 39.0 14-15 37.96549640163276 40.0 37.0 41.0 33.0 41.0 16-17 38.070167178013975 40.0 37.0 41.0 33.0 41.0 18-19 38.13705083772541 40.0 37.0 41.0 33.0 41.0 20-21 38.05760359098217 40.0 37.0 41.0 33.0 41.0 22-23 37.951388284997506 40.0 37.0 41.0 33.0 41.0 24-25 37.78643475198163 39.5 37.0 41.0 32.5 41.0 26-27 37.49529911523297 39.0 36.5 41.0 31.5 41.0 28-29 37.40668716425763 39.0 36.0 41.0 32.0 41.0 30-31 37.27128966583187 39.0 36.0 40.0 31.0 41.0 32-33 37.02138503818608 39.0 36.0 40.0 31.0 41.0 34-35 36.93477011657495 39.0 35.5 40.0 31.0 41.0 36-37 36.718522207010224 38.5 35.5 40.0 30.5 41.0 38-39 36.486300028972224 38.0 35.0 40.0 30.0 41.0 40-41 36.79529181755454 39.0 35.0 40.5 30.0 41.0 42-43 36.691895075989805 39.0 35.0 41.0 30.0 41.0 44-45 36.53294770710877 39.0 35.0 41.0 30.0 41.0 46-47 36.27334568840486 39.0 35.0 40.5 30.0 41.0 48-49 35.974077728577925 39.0 35.0 40.0 28.5 41.0 50-51 35.66896135682632 39.0 35.0 40.0 28.0 41.0 52-53 35.61721513599683 38.5 35.0 40.0 28.0 41.0 54-55 35.44057395585298 38.0 34.5 40.0 27.5 41.0 56-57 35.09949597950357 38.0 34.0 40.0 26.5 41.0 58-59 34.82265308628434 38.0 34.0 40.0 26.0 41.0 60-61 34.182541593489596 37.5 33.0 40.0 24.0 41.0 62-63 34.1082479497675 37.0 33.0 40.0 25.5 41.0 64-65 33.70499397176803 36.5 32.5 39.5 24.0 41.0 66-67 33.273290628076666 36.0 32.0 39.0 23.0 40.0 68-69 32.88696835647979 36.0 32.0 39.0 22.0 40.0 70-71 32.33606311661559 35.0 31.0 38.0 21.5 40.0 72-73 31.8770588083634 35.0 31.0 37.0 20.5 39.5 74-75 31.572854427921005 35.0 31.0 37.0 20.0 39.0 76-77 30.646393614050695 34.0 29.5 36.0 20.0 38.5 78-79 31.01450402661424 34.0 31.0 36.0 20.0 38.5 80-81 30.705643115558082 34.0 30.5 36.0 18.0 37.5 82-83 30.358190158272226 34.0 30.0 35.0 15.5 37.0 84-85 30.025875076855044 34.0 30.0 35.0 14.0 36.5 86-87 29.873069993475788 34.0 30.0 35.0 10.5 36.0 88-89 29.536706229988738 34.0 29.5 35.0 7.0 36.0 90-91 29.14769517903128 34.0 29.0 35.0 3.5 35.5 92-93 28.899389704330407 34.0 29.0 35.0 2.0 35.0 94-95 28.507837575527695 34.0 29.0 35.0 2.0 35.0 96-97 28.06197280281979 34.0 29.0 35.0 2.0 35.0 98-99 27.807698089362916 34.0 29.0 35.0 2.0 35.0 100-101 26.730444079026427 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.02167849452026971 1101 2 0.0018787416749859176 1101 3 0.06599888350229222 1101 4 0.08953475106360287 1101 5 0.028098337021553732 1101 6 0.07807182485937147 1101 7 0.033850785284471385 1101 8 0.055840274411480095 1101 9 0.07159279727697054 1101 10-11 0.05120522452838827 1101 12-13 0.07633888446699899 1101 14-15 0.006139088038146667 1101 16-17 0.0019333194627577655 1101 18-19 -0.05938826171778544 1101 20-21 0.04756836576385837 1101 22-23 0.016500940130697472 1101 24-25 0.03649125763094929 1101 26-27 0.230750981932772 1101 28-29 0.10976449654446441 1101 30-31 0.2924022367323076 1101 32-33 0.1970333839850298 1101 34-35 0.05617905093436093 1101 36-37 0.04684453554949641 1101 38-39 0.012052971127367584 1101 40-41 0.08629380951256849 1101 42-43 0.04356284515290554 1101 44-45 0.047938953149483154 1101 46-47 -0.06540541463762395 1101 48-49 -0.06349547654053822 1101 50-51 -0.05192233351406372 1101 52-53 -0.11784133365548399 1101 54-55 0.04422909953871823 1101 56-57 -0.025037655405675707 1101 58-59 -0.11176088597645872 1101 60-61 0.09413929239850205 1101 62-63 -0.13224271767246876 1101 64-65 -0.2861616469881696 1101 66-67 -0.25209171689912324 1101 68-69 -0.3663508306113883 1101 70-71 -0.16890951962638212 1101 72-73 -0.17828879186842173 1101 74-75 -0.4130554668016835 1101 76-77 -0.2918690681728897 1101 78-79 -0.25841934852392257 1101 80-81 -0.30825657842681764 1101 82-83 -0.2259341026141648 1101 84-85 -0.2530143520059944 1101 86-87 -0.2899435607187435 1101 88-89 -0.20485750062134045 1101 90-91 -0.12345648050057534 1101 92-93 -0.03743759313013584 1101 94-95 -0.014724815548074588 1101 96-97 0.11960863264358323 1101 98-99 -0.18033529684781868 1101 100-101 -0.0171213942263293 1102 1 -0.0048078223958221145 1102 2 0.009165929584415977 1102 3 0.04598744248810149 1102 4 -0.06021779087140544 1102 5 -0.11771443631719336 1102 6 0.061252426804344395 1102 7 -0.07182063616927792 1102 8 -0.10621726281794963 1102 9 -0.01923667625184322 1102 10-11 0.013370344197703332 1102 12-13 0.10330018800448215 1102 14-15 0.15794898267009927 1102 16-17 0.10817180924888703 1102 18-19 0.026399599735228207 1102 20-21 0.017537733371021602 1102 22-23 -0.021050867365012493 1102 24-25 0.06309604440365746 1102 26-27 -0.03944739049068602 1102 28-29 0.12209754077689894 1102 30-31 -0.01857301746119333 1102 32-33 -0.0020703417258829404 1102 34-35 0.005501632174791382 1102 36-37 -0.09304511402331173 1102 38-39 -0.07036237459424655 1102 40-41 0.06746263828487287 1102 42-43 0.03152911261429381 1102 44-45 0.13497000996703434 1102 46-47 0.18474368761182802 1102 48-49 0.07822565796519143 1102 50-51 0.17165667407022767 1102 52-53 0.15754572323588434 1102 54-55 0.09978426552569175 1102 56-57 0.12872145588586648 1102 58-59 -0.001868468416354574 1102 60-61 -0.003162289044915667 1102 62-63 -0.25617676576163717 1102 64-65 -0.19703836083363768 1102 66-67 -0.01459877526674802 1102 68-69 0.002173463090521466 1102 70-71 0.06934960089179043 1102 72-73 0.09435814212430671 1102 74-75 -0.024245701342476167 1102 76-77 0.06238311392376872 1102 78-79 0.1100505080943357 1102 80-81 0.12622479301862555 1102 82-83 -0.008141111158014525 1102 84-85 -0.05382740214347237 1102 86-87 -0.08482333780349904 1102 88-89 -0.11948631352385419 1102 90-91 0.040530712944828906 1102 92-93 0.0630595373896945 1102 94-95 -0.006606677849934073 1102 96-97 0.013687169670028254 1102 98-99 0.07973224204240736 1102 100-101 0.23134255735234532 1103 1 -3.9750342628508406E-4 1103 2 0.040743555073277093 1103 3 0.09401729430540584 1103 4 0.013279392580933802 1103 5 0.006812491313681335 1103 6 -0.018803545353094364 1103 7 0.041231999186472024 1103 8 0.03697315015610769 1103 9 -0.029028574640847182 1103 10-11 -0.002385818121545924 1103 12-13 0.006161226359758132 1103 14-15 0.14247124595451766 1103 16-17 -0.009137221643442217 1103 18-19 -0.06185360251801342 1103 20-21 0.10169653642796561 1103 22-23 0.0927572127182259 1103 24-25 -0.06562431008666891 1103 26-27 0.045485694077498806 1103 28-29 -0.08986704606963514 1103 30-31 -0.04178316420870942 1103 32-33 0.022248125498116167 1103 34-35 -0.1869229309912228 1103 36-37 -0.1922410524539373 1103 38-39 -0.05171772603481628 1103 40-41 0.06378964485083571 1103 42-43 0.2362614508870564 1103 44-45 0.21437422940659445 1103 46-47 0.25458850839328306 1103 48-49 0.18284389914779098 1103 50-51 0.4102893029676409 1103 52-53 0.30679583984454695 1103 54-55 0.1256295886213863 1103 56-57 0.12167434598668336 1103 58-59 0.16706321684790026 1103 60-61 0.2555060400426683 1103 62-63 0.09915799079536214 1103 64-65 -0.11282286802750008 1103 66-67 -0.23220865650531408 1103 68-69 -0.022949424498733606 1103 70-71 0.017162739996955167 1103 72-73 0.12602421079784065 1103 74-75 0.13049614502113016 1103 76-77 0.08702593262421132 1103 78-79 0.026827803490984792 1103 80-81 0.07519486944215359 1103 82-83 0.034487606864299636 1103 84-85 -0.23757000197842615 1103 86-87 -0.12055115524815463 1103 88-89 -0.15081726684450913 1103 90-91 -0.17286421368141092 1103 92-93 -0.15916930262322282 1103 94-95 -0.27321282761133503 1103 96-97 -0.20422069154772515 1103 98-99 -0.4003023817601168 1103 100-101 -0.4283417022723519 1104 1 -0.03172297825519266 1104 2 -0.045032570389359705 1104 3 0.03758100181606139 1104 4 0.04925719995011235 1104 5 0.13539942930982818 1104 6 0.04315665589925999 1104 7 0.07092067049692474 1104 8 -0.044881575131107354 1104 9 -0.06717527657702504 1104 10-11 -0.05463189360365561 1104 12-13 -0.021760389977650618 1104 14-15 -0.007228205742286775 1104 16-17 -0.0042056798680576435 1104 18-19 -0.005576833419198124 1104 20-21 -0.03231523608648246 1104 22-23 0.11262419688436154 1104 24-25 0.0776573129573066 1104 26-27 0.17110664017418742 1104 28-29 0.17318217512366374 1104 30-31 0.010026083921694351 1104 32-33 0.03289652832429368 1104 34-35 0.060102936393391815 1104 36-37 0.11737185130824912 1104 38-39 0.19661134872259822 1104 40-41 0.21716315752472326 1104 42-43 0.3243039863009045 1104 44-45 0.39391771884306337 1104 46-47 0.32948188233550724 1104 48-49 0.4176216264261825 1104 50-51 0.5277497875861954 1104 52-53 0.5681079708276258 1104 54-55 0.20601846542429314 1104 56-57 0.08731706070413026 1104 58-59 0.2948802509668482 1104 60-61 0.47308607194042196 1104 62-63 0.4024237342471082 1104 64-65 0.2079771114648281 1104 66-67 0.1828440121945718 1104 68-69 0.17592999729566117 1104 70-71 0.2546762674419014 1104 72-73 0.2849796564864491 1104 74-75 0.303441670814383 1104 76-77 0.3160253104656654 1104 78-79 0.40037026892931493 1104 80-81 0.4774683462275995 1104 82-83 0.4251465337308815 1104 84-85 0.4970177175264503 1104 86-87 0.538174791534427 1104 88-89 0.26862099604449696 1104 90-91 0.32351957638649864 1104 92-93 0.31719317608967046 1104 94-95 0.39834323386181225 1104 96-97 0.457556118427874 1104 98-99 0.4351714979734531 1104 100-101 0.4274886438223895 1105 1 0.011183678100600503 1105 2 0.0761004481469243 1105 3 0.12213167423875504 1105 4 0.11475915097751965 1105 5 0.02896639618877117 1105 6 0.07913070574765868 1105 7 0.09324362044892354 1105 8 0.1106301265539571 1105 9 0.2241900340876768 1105 10-11 0.0934717629467201 1105 12-13 0.11645437605135811 1105 14-15 0.20240546910893897 1105 16-17 0.18717014831969436 1105 18-19 0.1554224351918876 1105 20-21 0.16527775619720586 1105 22-23 0.20884233210423275 1105 24-25 0.2317319483110154 1105 26-27 0.3545423655661324 1105 28-29 0.23087643305775885 1105 30-31 0.17540875871235784 1105 32-33 0.22480683302428162 1105 34-35 0.2098799899890409 1105 36-37 0.2218894230970676 1105 38-39 0.3102063312728234 1105 40-41 0.25973959590798046 1105 42-43 0.19517675437320747 1105 44-45 0.2012930467765628 1105 46-47 -0.02878759631428096 1105 48-49 0.023755438703986442 1105 50-51 0.1304227614221105 1105 52-53 -0.0052067797565698015 1105 54-55 -0.010589025990576317 1105 56-57 -0.025952899924142514 1105 58-59 -0.08768775189706446 1105 60-61 -0.037694120428120925 1105 62-63 -0.23767540639779838 1105 64-65 -0.28786362922680553 1105 66-67 -0.273950827303004 1105 68-69 -0.1788673549403086 1105 70-71 -0.10353085860102595 1105 72-73 -0.09742870220301825 1105 74-75 -0.17946547289512083 1105 76-77 -0.23530763776267705 1105 78-79 -0.03816654473063252 1105 80-81 0.06938083685231078 1105 82-83 0.19357808974018553 1105 84-85 0.15284378027109113 1105 86-87 0.05268100009816479 1105 88-89 0.2975451698993332 1105 90-91 0.2757792491929827 1105 92-93 0.17572908671186482 1105 94-95 0.3282507513286035 1105 96-97 0.26592747550369467 1105 98-99 0.3342935804358511 1105 100-101 0.43890015633537516 1106 1 -0.021006138036433697 1106 2 -0.030201665081477813 1106 3 -0.0703851109753515 1106 4 -0.038662342086624335 1106 5 -0.0081380644889677 1106 6 0.033512532507415926 1106 7 0.031861384336757226 1106 8 -0.011085043325437027 1106 9 -0.12014539630188636 1106 10-11 -0.0912386178996698 1106 12-13 -0.15695001823435462 1106 14-15 0.018508683147850036 1106 16-17 -0.023112401468281973 1106 18-19 0.055750604639627 1106 20-21 -0.0919129007567463 1106 22-23 -0.1266471469070254 1106 24-25 -0.10502042841360293 1106 26-27 0.053536228253022955 1106 28-29 -0.06794506680724766 1106 30-31 -0.03599054458953077 1106 32-33 0.12613877992330202 1106 34-35 0.18156378485498692 1106 36-37 0.004417377704470482 1106 38-39 0.27627597767075684 1106 40-41 0.05400516881275763 1106 42-43 0.02874266726531971 1106 44-45 0.14404270575794698 1106 46-47 -0.10426192389765987 1106 48-49 -0.06494479956773347 1106 50-51 0.29130439093830063 1106 52-53 0.16864182600175326 1106 54-55 0.059686358736584566 1106 56-57 0.1517033521953195 1106 58-59 0.09579745186213984 1106 60-61 0.21598238636688194 1106 62-63 0.026242529128168712 1106 64-65 0.20020420323638177 1106 66-67 0.11666186529748757 1106 68-69 0.03581940313167564 1106 70-71 0.3111159118908873 1106 72-73 0.2782711501290933 1106 74-75 0.13550091951348264 1106 76-77 -0.08784461001534893 1106 78-79 0.040468697000047626 1106 80-81 0.060534142210443065 1106 82-83 0.14391284636131374 1106 84-85 0.16696727462813854 1106 86-87 0.11016909255123153 1106 88-89 0.2307761310636245 1106 90-91 0.01618664310464979 1106 92-93 0.02691824338829818 1106 94-95 0.18908161594295692 1106 96-97 0.22732803306286087 1106 98-99 0.1951586351744261 1106 100-101 0.20221084552466095 1107 1 -0.06592782392171159 1107 2 -0.045830875588343645 1107 3 -0.08445325293518735 1107 4 -0.12920240801665983 1107 5 -0.0954652779395957 1107 6 -0.08570085242355674 1107 7 -0.11878574102637174 1107 8 -0.10685031545153834 1107 9 -0.12966535530998868 1107 10-11 -0.014726301710531686 1107 12-13 -0.022810801247544532 1107 14-15 -0.007552223734812458 1107 16-17 -0.12584299186119807 1107 18-19 -0.16259932260662424 1107 20-21 -0.12556639892343213 1107 22-23 -0.2970559027942201 1107 24-25 -0.09180520205580223 1107 26-27 -0.1674656439215454 1107 28-29 -0.2843076586852149 1107 30-31 -0.20004521292739952 1107 32-33 -0.2000517831174946 1107 34-35 -0.2709174910120282 1107 36-37 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-0.654989566582973 2316 80-81 -0.6779568144748538 2316 82-83 -0.7844860001613547 2316 84-85 -0.8980408762478298 2316 86-87 -0.6086870302531509 2316 88-89 -0.5744020333122641 2316 90-91 -0.6245794253584087 2316 92-93 -0.44959258007685676 2316 94-95 -0.6550490765366384 2316 96-97 -0.40329987982514837 2316 98-99 -0.7277298023562615 2316 100-101 -0.975931077050717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 9.0 4 10.0 5 37.0 6 96.0 7 269.0 8 669.0 9 1248.0 10 2149.0 11 3571.0 12 5720.0 13 8255.0 14 9496.0 15 11811.0 16 14743.0 17 18843.0 18 17432.0 19 13280.0 20 12670.0 21 13020.0 22 13781.0 23 15401.0 24 17788.0 25 21385.0 26 26646.0 27 33758.0 28 42337.0 29 53162.0 30 65985.0 31 82064.0 32 102758.0 33 128918.0 34 168843.0 35 224335.0 36 318116.0 37 411560.0 38 358707.0 39 69477.0 40 50.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.85976328748054 19.436728958437808 13.633551601554183 19.06995615252747 2 14.100510163502289 25.975380699790147 37.0580862706847 22.866022866022867 3 27.056270337550327 25.489336270669817 28.76861392722441 18.685779464555445 4 12.158500331454436 16.702157272398736 41.97895559297133 29.1603868031755 5 11.513464216685989 41.216763335414846 33.74900967881912 13.520762769080042 6 26.50564659807924 40.284455872282834 17.09169281702383 16.118204712614094 7 27.08242749625393 34.18962340046469 21.807779150401657 16.92016995287972 8 23.7049570463892 30.076398390315674 19.274086380915218 26.944558182379907 9 22.21461379768126 23.450281179112558 24.434506395082327 29.900598628123852 10-11 25.406518897759256 25.229844698878207 27.862303371842163 21.50133303152037 12-13 30.92531066479228 23.334807435241846 25.78009778889084 19.959784111075034 14-15 22.792289970389067 27.108140209688752 30.1089346706614 19.990635149260775 16-17 19.546635005521328 29.348640687222815 29.84444583309117 21.26027847416469 18-19 18.98222294276479 33.75582667183477 28.245052545469562 19.016897839930884 20-21 22.545652484115294 27.393850476492034 26.607705817221365 23.452791222171307 22-23 20.088498552918438 28.515503633763167 27.766726868872084 23.629270944446315 24-25 24.367931466496884 28.150160876665304 27.580614219810446 19.90129343702737 26-27 23.59472291271165 24.889740201168415 32.31975603872335 19.19578084739659 28-29 19.5103942346878 32.576876474296576 29.006071452374005 18.90665783864162 30-31 21.79103094761417 27.80270322359873 27.303436783402535 23.10282904538456 32-33 22.17970795605291 28.605990206933114 28.693415037639003 20.52088679937498 34-35 24.946972870343725 26.190387225329083 29.443468064961536 19.419171839365653 36-37 23.63058551154871 25.527772915324025 31.519299352102625 19.322342221024638 38-39 20.858110256351424 28.919626731871684 31.673293148338132 18.548969863438757 40-41 21.041899847864975 29.989087010658196 28.25378185122235 20.715231290254486 42-43 19.5240864901619 30.727180880606923 26.264322786367238 23.48440984286394 44-45 22.71004750482761 26.0240019332293 27.02122750446928 24.244723057473806 46-47 24.931223705671766 29.72258971616601 26.718850199757693 18.62733637840454 48-49 23.87594995453153 28.638843138805775 28.103993228453604 19.38121367820909 50-51 18.84118547508542 29.496538846591 31.763626011023426 19.898649667300152 52-53 21.914163570251517 32.13543617175152 26.046747092618027 19.90365316537894 54-55 19.935050662056746 28.520813022554197 27.725453472056227 23.818682843332827 56-57 23.356292173841606 26.686762480114123 27.97261195247885 21.98433339356542 58-59 27.475737892106405 26.37968513719341 25.926222915770385 20.2183540549298 60-61 22.321614894318593 28.464787104305202 31.07651973221448 18.137078269161726 62-63 20.670051187379375 28.328774894408546 30.049399841962465 20.951774076249617 64-65 19.85206651782533 33.242037957600765 27.19603995196391 19.70985557260999 66-67 22.295900972961633 27.681108109761958 25.995493605377952 24.027497311898454 68-69 20.483052838000972 29.50577104807477 26.793023395550314 23.218152718373947 70-71 24.370551736524277 28.741675927168103 26.223161843267306 20.66461049304031 72-73 23.33998572801334 25.75317940621369 30.569232026407985 20.337602839364987 74-75 19.98550078023981 32.95463334846764 27.507331544892306 19.55253432640025 76-77 22.017751275018036 27.959700209622536 26.217150068672463 23.80539844668696 78-79 22.273257417085045 29.034927912483795 27.565669273583847 21.126145396847313 80-81 24.512295389111642 27.613678299474504 27.700354636659657 20.1736716747542 82-83 22.845120103618292 26.673735655364293 29.941369490198166 20.53977475081924 84-85 20.37887219743777 29.38189920717428 29.75849470174974 20.480733893638213 86-87 21.367093154505305 30.12077097301542 26.484269883380435 22.027865989098842 88-89 20.224751015884905 30.215840856424077 25.489851201647962 24.069556926043052 90-91 22.685008165097084 25.30976896948478 26.813199970809286 25.19202289460885 92-93 24.12957705365192 29.073557539572427 26.907632803545184 19.889232603230468 94-95 22.75704150725727 28.374821654478353 28.17985968331516 20.688277154949212 96-97 18.193350699246764 29.07326588856537 31.351154588529674 21.382228823658195 98-99 21.308386751693227 31.60021727132215 25.91019510283025 21.18120087415437 100-101 19.37341794969558 28.729126768318462 27.063791108727074 24.833664173258885 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 330.0 1 254.0 2 406.0 3 630.5 4 508.5 5 392.5 6 503.0 7 798.5 8 1165.0 9 1443.0 10 1490.0 11 1506.0 12 1607.0 13 1841.5 14 2091.0 15 2306.0 16 2597.5 17 2834.5 18 3050.0 19 3320.0 20 3668.5 21 4111.0 22 4933.0 23 6065.0 24 7278.0 25 8887.5 26 11198.5 27 14547.0 28 19171.0 29 23995.0 30 28555.5 31 33126.5 32 37633.0 33 41537.5 34 45436.5 35 49743.5 36 53850.0 37 57864.0 38 61298.0 39 63512.0 40 65245.5 41 66636.0 42 68274.5 43 70738.0 44 72223.0 45 74243.0 46 82899.0 47 142981.5 48 281575.5 49 275959.0 50 139290.5 51 77466.0 52 54026.5 53 42733.0 54 35818.5 55 30556.0 56 25127.5 57 19530.5 58 15179.0 59 12162.5 60 9600.5 61 7152.5 62 5086.5 63 3426.0 64 2149.5 65 1299.0 66 766.5 67 399.5 68 220.5 69 134.5 70 72.0 71 42.5 72 23.0 73 11.5 74 9.5 75 8.0 76 3.5 77 4.0 78 3.0 79 0.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05034087150011864 2 0.0049379500690220545 3 0.04658278560688062 4 0.0 5 0.0 6 0.0013109601953155896 7 0.0 8 0.0 9 0.0 10-11 2.6219203906311793E-4 12-13 0.0 14-15 0.0024034270247452476 16-17 0.0 18-19 0.0 20-21 0.011820491094428901 22-23 0.0 24-25 0.0 26-27 0.007297678420590116 28-29 0.010116242840518633 30-31 0.01057507890887909 32-33 0.006445554293634983 34-35 0.00801870652801369 36-37 0.020079540324917114 38-39 0.010116242840518633 40-41 0.013109601953155896 42-43 0.0 44-45 0.0 46-47 6.554800976577948E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.00124541218554981 58-59 2.4034270247452475E-4 60-61 0.009810352128278329 62-63 0.014639055514357416 64-65 0.04160113686468138 66-67 0.023553584842503427 68-69 0.0015950015709673008 70-71 2.6219203906311793E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.1849336588593162E-5 82-83 0.0 84-85 8.739734635437265E-5 86-87 7.865761171893538E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.747946927087453E-4 96-97 3.495893854174906E-4 98-99 6.991787708349812E-4 100-101 0.0013765082050813692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2288399.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.207672987660715 #Duplication Level Percentage of deduplicated Percentage of total 1 80.62155138389294 38.05955833488702 2 12.205921047207262 11.524262586195313 3 3.0451389568868037 4.3126177223609545 4 1.247416261606037 2.355504758295521 5 0.6893551328402823 1.6271425841744724 6 0.412866174427748 1.1694270810030967 7 0.29625532180194536 0.9789867046736301 8 0.20550913771144605 0.7761286535246462 9 0.1639270739205614 0.6964754129519353 >10 0.9887534670443515 8.697216199546675 >50 0.07676066854616281 2.476784857958982 >100 0.03815148779221699 3.4610348765812136 >500 0.0028912275110597233 0.9657195119809778 >1k 0.003357554528972582 3.6335780014938512 >5k 9.326540358257172E-4 3.0495849492956344 >10k+ 0.0012124502465734324 16.215977765076044 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 110332 4.8213620089853215 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 33163 1.44917909857503 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 32563 1.422959894668718 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 30359 1.3266480189861996 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 27101 1.1842777417749264 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 22118 0.966527253333007 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 21617 0.9446342180712367 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 19288 0.8428600082415697 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 17891 0.7818129618130405 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 16990 0.7424404572803956 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 13970 0.6104704642852928 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 12167 0.5316817565468259 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 10748 0.4696733393083986 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8747 0.38223229428084876 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 7870 0.34390855790445635 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 7832 0.34224800832372326 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 7182 0.3138438707585522 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7055 0.3082941392650495 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6888 0.30099646084445936 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 6569 0.287056584100937 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 6200 0.27093177369855515 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5915 0.2584776518430571 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 5006 0.21875555792499474 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4875 0.21303103173878332 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4557 0.19913485366843806 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4482 0.1958574531801491 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 4391 0.19188087392102512 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 3730 0.16299605095090497 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 3608 0.15766481282328823 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3239 0.1415400024209065 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2950 0.12891108587269964 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 2834 0.12384203978414604 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 2794 0.12209409285705859 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2635 0.11514600382188594 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2401 0.10492051429842435 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.092466829429658E-4 0.0 12-13 0.0 0.0 0.0 2.184933658859316E-4 0.0 14-15 0.0 2.184933658859316E-5 0.0 3.277400488288974E-4 0.0 16-17 0.0 4.369867317718632E-5 0.0 3.9328805859467685E-4 0.0 18-19 0.0 6.554800976577948E-5 0.0 5.46233414714829E-4 0.0 20-21 0.0 8.739734635437264E-5 0.0 9.39521473309506E-4 0.0 22-23 0.0 8.739734635437264E-5 0.0 0.001638700244144487 0.0 24-25 0.0 8.739734635437264E-5 0.0 0.0025563723808653994 0.0 26-27 0.0 8.739734635437264E-5 0.0 0.0033647978346433467 0.0 28-29 0.0 8.739734635437264E-5 0.0 0.00629260893751483 0.0 30-31 0.0 8.739734635437264E-5 0.0 0.012191929816434983 0.0 32-33 0.0 8.739734635437264E-5 0.0 0.02453680498899012 0.0 34-35 0.0 8.739734635437264E-5 0.0 0.04074901273772624 0.0 36-37 0.0 8.739734635437264E-5 0.0 0.05790074195977188 0.0 38-39 0.0 8.739734635437264E-5 0.0 0.0814543268022753 0.0 40-41 0.0 1.092466829429658E-4 0.0 0.10612222781079697 0.0 42-43 0.0 1.3109601953155897E-4 0.0 0.12674800155042892 0.0 44-45 2.184933658859316E-5 1.3109601953155897E-4 0.0 0.1414963037477293 0.0 46-47 4.369867317718632E-5 1.3109601953155897E-4 0.0 0.16212207748736124 0.0 48-49 4.369867317718632E-5 1.3109601953155897E-4 0.0 0.17641154361630118 0.0 50-51 4.369867317718632E-5 1.3109601953155897E-4 0.0 0.19083210576477266 0.0 52-53 4.369867317718632E-5 1.3109601953155897E-4 0.0 0.22764823791655214 0.0 54-55 4.369867317718632E-5 1.3109601953155897E-4 0.0 0.2865758986959879 0.0 56-57 4.369867317718632E-5 1.3109601953155897E-4 0.0 0.3945334707802267 0.0 58-59 4.369867317718632E-5 1.3109601953155897E-4 0.0 0.5382365575234039 0.0 60-61 4.369867317718632E-5 1.3109601953155897E-4 0.0 0.6884944452431591 0.0 62-63 4.369867317718632E-5 1.3109601953155897E-4 0.0 0.750633958501118 0.0 64-65 4.369867317718632E-5 1.3109601953155897E-4 0.0 0.8315639012252671 0.0 66-67 4.369867317718632E-5 1.3109601953155897E-4 0.0 1.124476107531947 0.0 68-69 4.369867317718632E-5 1.3109601953155897E-4 0.0 1.453636363239103 0.0 70-71 4.369867317718632E-5 1.3109601953155897E-4 0.0 1.6589327298255243 0.0 72-73 4.369867317718632E-5 1.3109601953155897E-4 0.0 1.8884381613521068 0.0 74-75 4.369867317718632E-5 1.3109601953155897E-4 0.0 2.0778937589117983 0.0 76-77 4.369867317718632E-5 1.3109601953155897E-4 0.0 2.201429907983704 0.0 78-79 4.369867317718632E-5 1.3109601953155897E-4 0.0 2.3250097557287868 0.0 80-81 4.369867317718632E-5 1.3109601953155897E-4 0.0 2.449922413005774 0.0 82-83 4.369867317718632E-5 1.3109601953155897E-4 0.0 2.6595012495635597 0.0 84-85 4.369867317718632E-5 1.3109601953155897E-4 0.0 3.1729825087320873 0.0 86-87 4.369867317718632E-5 1.3109601953155897E-4 0.0 4.023380538096721 0.0 88-89 4.369867317718632E-5 1.3109601953155897E-4 0.0 5.010642811852303 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 3020 0.0 51.91654 2 ACCTGGG 3130 0.0 48.722652 3 GTACCTG 3580 0.0 44.216854 1 TATAACG 380 0.0 42.51029 2 CCTGGGG 2120 0.0 37.186954 4 AGTACGC 1125 0.0 33.785954 2 TACGCAG 1155 0.0 33.717102 4 ATAACGC 470 0.0 33.35695 3 GTATAAC 675 0.0 33.099445 1 GTACGCA 1165 0.0 32.62378 3 GAGTACG 1255 0.0 32.195995 1 GTACCCG 340 0.0 30.758873 1 TACCCGG 315 0.0 30.166029 2 ACGCAGT 1305 0.0 29.841576 5 CGCAGTG 1410 0.0 27.95615 6 ACGTGCG 85 6.4131693E-4 27.93817 9 TAACGCA 615 0.0 27.800062 4 CTGGGGG 1255 0.0 26.489435 5 GTACACG 305 0.0 23.378576 1 TCACGCA 310 0.0 22.97989 4 >>END_MODULE