FastQCFastQC Report
Wed 25 May 2016
SRR1003846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1628933
Sequences flagged as poor quality0
Sequence length121
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT132910.815932883672932No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT128620.7895966255211233No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC78820.4838750273952336No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT75690.46465999522386736No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT49520.3040026815099209No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37890.23260625206807153No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35260.21646071385379262No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC33840.20774335101566488No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA33640.20651555343282996No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31020.19043140509769277No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29250.17956539648960393No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT25820.1585086679439854No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC25720.15789476915256798No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25000.15347469785436232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24820.1523696800298109No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24190.148502117643881No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23850.14641486175306168No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA21140.1297782045056488No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA20410.1252967433283014No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT20080.12327087731662381No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC19540.1199558238429696No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA19410.11915775541412692No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG21350.056.84922
ACCTGGG22250.053.7057883
TATAACG2650.052.0960922
TAACGCA3000.045.962614
GTACCCG2250.041.0196041
GTACCTG31900.039.4202881
TACCCGG3000.032.5962372
CCTGGGG18050.031.8300634
GTACCGG2007.4578566E-1131.7260951
ATAACGC4800.031.118593
GTATAAC6400.026.1379781
GTATAGG8150.024.772191
TCACGCA3051.6370905E-1124.4882774
ACCCGGG3800.024.1893483
CCGATCG600.001579039823.94071424-25
CTGGGGG12600.023.254895
TATCACG3304.5474735E-1122.6604842
CCTGGGA15650.022.026814
GTACATG61100.021.7140391
TACCGGG2706.497976E-821.304732
Produced by FastQC (version 0.11.5)