##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003846_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1628933 Sequences flagged as poor quality 0 Sequence length 121 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.774126989876194 31.0 31.0 34.0 27.0 34.0 2 30.73442247164248 31.0 31.0 34.0 27.0 34.0 3 30.81193885813597 31.0 31.0 34.0 27.0 34.0 4 34.330014801099864 37.0 35.0 37.0 30.0 37.0 5 34.2880388573379 37.0 35.0 37.0 30.0 37.0 6 34.382513584045505 37.0 35.0 37.0 30.0 37.0 7 34.396862240497306 37.0 35.0 37.0 30.0 37.0 8 34.37328116012138 37.0 35.0 37.0 30.0 37.0 9 35.83573910038043 39.0 35.0 39.0 30.0 39.0 10-11 35.684773099937196 39.0 35.0 39.0 30.0 39.0 12-13 35.547964526472235 39.0 35.0 39.0 30.0 39.0 14-15 36.54623793612137 39.0 36.0 41.0 30.0 41.0 16-17 36.597684496538534 39.0 36.0 41.0 30.0 41.0 18-19 36.5482174527743 39.0 36.0 41.0 30.0 41.0 20-21 36.44962899026541 39.0 36.0 41.0 29.5 41.0 22-23 36.373859452782895 39.0 36.0 41.0 29.0 41.0 24-25 36.18344769244653 39.0 36.0 41.0 28.5 41.0 26-27 36.01457395730825 39.0 35.5 40.5 27.0 41.0 28-29 35.83382926124033 39.0 35.0 40.0 27.0 41.0 30-31 35.61415632196045 38.5 35.0 40.0 27.0 41.0 32-33 35.384122919727204 38.0 34.5 40.0 26.5 41.0 34-35 35.39202932226188 38.0 35.0 40.0 26.5 41.0 36-37 35.271143441749906 38.0 34.5 40.0 25.5 41.0 38-39 35.27676153653956 38.0 34.5 40.0 25.5 41.0 40-41 35.381730863086446 39.0 35.0 40.0 26.0 41.0 42-43 35.2663083748687 39.0 35.0 40.0 25.5 41.0 44-45 35.07063028375016 38.0 34.5 40.0 24.5 41.0 46-47 34.815970638448604 38.0 34.0 40.0 24.0 41.0 48-49 34.57151890225074 38.0 34.0 40.0 23.0 41.0 50-51 33.80978652897326 37.5 33.0 39.5 22.5 40.5 52-53 33.96593997420398 38.0 33.0 40.0 23.0 40.5 54-55 34.31177863055141 38.0 34.0 40.0 23.0 41.0 56-57 34.15196327902989 38.0 33.5 40.0 22.5 41.0 58-59 33.88565244856602 38.0 33.0 40.0 21.0 41.0 60-61 33.61669325871598 38.0 33.0 40.0 20.0 41.0 62-63 33.37712355265686 37.0 32.5 40.0 19.0 41.0 64-65 33.083471818669025 37.0 32.0 40.0 18.0 41.0 66-67 32.727971930091655 36.0 32.0 39.0 17.5 41.0 68-69 32.357407579071705 36.0 31.0 39.0 13.5 41.0 70-71 31.92719497978124 35.5 31.0 39.0 12.5 40.0 72-73 31.55546636970336 35.0 31.0 38.0 9.0 40.0 74-75 31.178696729699748 35.0 31.0 37.0 7.5 39.5 76-77 30.729107028956992 35.0 30.0 37.0 7.0 39.0 78-79 30.16929916700073 34.0 29.5 36.0 4.0 39.0 80-81 29.753072409976348 34.0 29.0 36.0 2.0 38.5 82-83 29.410338239817108 34.0 29.0 35.5 2.0 37.0 84-85 29.156083460768492 34.0 29.0 35.0 2.0 37.0 86-87 28.904182676635564 34.0 29.0 35.0 2.0 36.5 88-89 28.57812291849941 34.0 29.0 35.0 2.0 36.0 90-91 28.21984084059934 34.0 27.5 35.0 2.0 36.0 92-93 27.963178043541383 34.0 27.0 35.0 2.0 35.0 94-95 27.72777241298445 34.0 26.5 35.0 2.0 35.0 96-97 27.425949993032248 34.0 26.0 35.0 2.0 35.0 98-99 27.152770555940606 33.5 25.0 35.0 2.0 35.0 100-101 26.8587308379166 33.0 25.0 35.0 2.0 35.0 102-103 26.5638393353195 33.0 25.0 35.0 2.0 35.0 104-105 26.24545024258211 33.0 24.0 35.0 2.0 35.0 106-107 25.9100239236359 32.5 23.0 35.0 2.0 35.0 108-109 25.5725373603457 32.0 21.0 35.0 2.0 35.0 110-111 25.19017141895953 32.0 19.0 34.5 2.0 35.0 112-113 24.686913458073477 32.0 17.5 34.0 2.0 35.0 114-115 24.15886165974905 31.5 8.5 34.0 2.0 35.0 116-117 23.47147734130256 31.0 2.0 34.0 2.0 35.0 118-119 22.681980167385646 31.0 2.0 34.0 2.0 35.0 120-121 21.007784236675175 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5836094903663671 1101 2 0.42546733831223804 1101 3 0.5345332691814413 1101 4 0.815000279198685 1101 5 0.7831633270408318 1101 6 0.9259388348879583 1101 7 0.7917199832796271 1101 8 0.7517295046358754 1101 9 0.9251610215326949 1101 10-11 1.0045244027642397 1101 12-13 1.0775897933068208 1101 14-15 1.1042534811758813 1101 16-17 1.0031011471162685 1101 18-19 0.8195757326909927 1101 20-21 0.9120543399488099 1101 22-23 1.0834487189208417 1101 24-25 0.7730411615938024 1101 26-27 0.9291070573060125 1101 28-29 0.9588357595080694 1101 30-31 0.9827775045655684 1101 32-33 0.9590847543633956 1101 34-35 1.070000897313335 1101 36-37 0.9350317096105201 1101 38-39 0.9220105820788191 1101 40-41 0.9947670532545132 1101 42-43 1.0340302575199303 1101 44-45 1.1478269168194188 1101 46-47 1.1699055802630411 1101 48-49 1.2232969966479246 1101 50-51 0.8019166299030616 1101 52-53 0.8802634470310977 1101 54-55 1.1444000118649527 1101 56-57 1.1130749398295592 1101 58-59 1.096499808582287 1101 60-61 1.0574619046948612 1101 62-63 1.0100478799843273 1101 64-65 0.8926127103197672 1101 66-67 0.8806732349075546 1101 68-69 0.9752912894292933 1101 70-71 0.6907555361859714 1101 72-73 0.7372383816259429 1101 74-75 0.7153238410370584 1101 76-77 0.5894650113167437 1101 78-79 0.8328538535455259 1101 80-81 0.7441734033061174 1101 82-83 0.9968109780664314 1101 84-85 1.0119217548878758 1101 86-87 0.8645238872345473 1101 88-89 0.9778070254296445 1101 90-91 0.9181244912047006 1101 92-93 1.028046799055005 1101 94-95 1.0644879035154666 1101 96-97 1.1101108819618197 1101 98-99 1.3287823201353497 1101 100-101 1.3495882215285882 1101 102-103 1.2069411399578023 1101 104-105 1.0475704638005077 1101 106-107 0.7493220425378979 1101 108-109 0.7849292580628529 1101 110-111 0.8315010064547437 1101 112-113 0.8951984975233529 1101 114-115 0.9370522428917667 1101 116-117 0.9372502573762596 1101 118-119 0.8701889661213045 1101 120-121 0.7803121116535898 1102 1 0.45692251569495923 1102 2 0.441096040292269 1102 3 0.5220026661465234 1102 4 0.6345179640861431 1102 5 0.6540608280280296 1102 6 0.7014586633981423 1102 7 0.5668031896879242 1102 8 0.7391983374897109 1102 9 0.7496496546911544 1102 10-11 0.8195912499465052 1102 12-13 0.6604924277063304 1102 14-15 0.8052090856210796 1102 16-17 0.7232198925328674 1102 18-19 0.5327643714905577 1102 20-21 0.7340572169161419 1102 22-23 0.6959505522823832 1102 24-25 0.8332278824151516 1102 26-27 0.9013293735467798 1102 28-29 0.9470839120437446 1102 30-31 0.957045570228118 1102 32-33 0.7339265211598089 1102 34-35 0.7350784626092164 1102 36-37 0.8087796647072665 1102 38-39 0.7696190324652292 1102 40-41 0.6795321009219109 1102 42-43 0.7576411335846984 1102 44-45 0.9341441165712112 1102 46-47 0.9758919287709702 1102 48-49 0.9181954566242396 1102 50-51 0.5408868448294513 1102 52-53 0.7320426538906872 1102 54-55 0.7709792412889982 1102 56-57 0.8025892400495636 1102 58-59 0.8315900663811249 1102 60-61 1.1778367566156618 1102 62-63 0.8850786240470541 1102 64-65 0.9103920864127346 1102 66-67 0.8258825201786024 1102 68-69 0.644955643239868 1102 70-71 0.6810925926535099 1102 72-73 0.6477407226875975 1102 74-75 0.631798709881199 1102 76-77 0.5660433945739207 1102 78-79 0.8144973916512406 1102 80-81 0.8899513127098544 1102 82-83 0.7316547192521909 1102 84-85 0.5910851779148985 1102 86-87 0.7247833784062081 1102 88-89 0.6296688745862973 1102 90-91 0.8578884106496147 1102 92-93 0.9455672480875528 1102 94-95 0.6758964892885828 1102 96-97 0.8261993346046808 1102 98-99 1.0143077897579573 1102 100-101 0.9156267503264672 1102 102-103 0.8672550678643809 1102 104-105 0.9288261754268383 1102 106-107 0.8820797003480187 1102 108-109 1.0356984237423212 1102 110-111 0.8071440932714644 1102 112-113 0.7854988867601094 1102 114-115 0.9815434145508171 1102 116-117 1.1209832635250727 1102 118-119 0.9977305693254586 1102 120-121 1.0229346648210722 1103 1 0.741877927480239 1103 2 0.7100795455670834 1103 3 0.8212000782815139 1103 4 1.0160129806974396 1103 5 1.1055150449657205 1103 6 0.9938617641733387 1103 7 0.9445848531489034 1103 8 0.8356744113954093 1103 9 0.9849990747529347 1103 10-11 1.0627841158321516 1103 12-13 1.1676344105704644 1103 14-15 1.2922878289911353 1103 16-17 1.2355853993230994 1103 18-19 1.1273324778028524 1103 20-21 1.1811519887293969 1103 22-23 1.1143879247834079 1103 24-25 1.0999044575739774 1103 26-27 1.2987190687149308 1103 28-29 1.2309957264635187 1103 30-31 1.2811202919160607 1103 32-33 1.1299211589169929 1103 34-35 1.0558350051283085 1103 36-37 1.0275457660070302 1103 38-39 1.0129596514545653 1103 40-41 1.1076063563278709 1103 42-43 1.1156757550220107 1103 44-45 1.1211322263806167 1103 46-47 1.1573910253496251 1103 48-49 1.3033988721397733 1103 50-51 0.8258303081388902 1103 52-53 1.0500271500147207 1103 54-55 1.0248307304770847 1103 56-57 0.9609898928443386 1103 58-59 0.9674969848400607 1103 60-61 0.9790674496272587 1103 62-63 1.0570072829034984 1103 64-65 0.9697599828981325 1103 66-67 0.9758810630474031 1103 68-69 0.8693252882827025 1103 70-71 0.8478230816667356 1103 72-73 0.7544478976016222 1103 74-75 0.5982934350662532 1103 76-77 0.6696888454103167 1103 78-79 0.9501041410829636 1103 80-81 0.7007775061003052 1103 82-83 0.8323235424730342 1103 84-85 0.8554070290543763 1103 86-87 0.7494893293300287 1103 88-89 0.7688100414569625 1103 90-91 0.8411899785227845 1103 92-93 0.6488641531408845 1103 94-95 0.5077557886998996 1103 96-97 0.4967944269867992 1103 98-99 0.5961280380531129 1103 100-101 0.5547964769686509 1103 102-103 0.5803730372063427 1103 104-105 0.7439558601036467 1103 106-107 0.5936583562901987 1103 108-109 0.5902076619541319 1103 110-111 0.8486446469813202 1103 112-113 0.6765605525524983 1103 114-115 0.7405342054816337 1103 116-117 0.5792839571195252 1103 118-119 0.6642293744778769 1103 120-121 0.8341038668960259 1104 1 0.5959770382288418 1104 2 0.716460622133372 1104 3 0.7202970820993073 1104 4 0.9008271638683922 1104 5 1.0310026381876156 1104 6 0.8716600787492581 1104 7 0.7386909930286123 1104 8 0.7808125253818687 1104 9 0.8179477952521381 1104 10-11 1.0918841760560127 1104 12-13 1.0569129359720932 1104 14-15 1.2530069826499926 1104 16-17 0.9693017479607704 1104 18-19 1.0711176649037384 1104 20-21 1.1360311160079135 1104 22-23 1.000731516669397 1104 24-25 1.0414329077711386 1104 26-27 1.0942908668281603 1104 28-29 0.9617581520804279 1104 30-31 0.9932057856823704 1104 32-33 1.1105580428055148 1104 34-35 0.938732303253289 1104 36-37 1.0516378717381798 1104 38-39 0.8546577110888478 1104 40-41 0.7723287086633732 1104 42-43 1.0011287994013855 1104 44-45 0.9370520327961813 1104 46-47 1.1571634527087369 1104 48-49 1.0383102032076224 1104 50-51 0.7774560509148927 1104 52-53 0.8029789630904745 1104 54-55 0.7877921227032019 1104 56-57 0.9106951335257705 1104 58-59 0.9672773998481645 1104 60-61 0.8806125925036881 1104 62-63 0.9376921529700724 1104 64-65 0.6231653437258586 1104 66-67 0.6652079363319459 1104 68-69 0.7842428105320778 1104 70-71 0.7004248850066013 1104 72-73 0.5630624715344013 1104 74-75 0.7701564441733773 1104 76-77 0.7943792109793897 1104 78-79 0.932167649222226 1104 80-81 1.052086515987508 1104 82-83 0.8696392764535794 1104 84-85 1.1617919939479044 1104 86-87 1.289756876386324 1104 88-89 1.2490599011456176 1104 90-91 1.2599783456122218 1104 92-93 1.4523499028736708 1104 94-95 1.2896213839967743 1104 96-97 1.3610559371889863 1104 98-99 1.5153724546864744 1104 100-101 1.4087524128769644 1104 102-103 1.2277823864329882 1104 104-105 1.2614471535662553 1104 106-107 1.1364230232715506 1104 108-109 1.0170391394401115 1104 110-111 1.11935149664653 1104 112-113 1.1491683421077887 1104 114-115 0.9444017648358951 1104 116-117 0.7126483066445779 1104 118-119 0.6053783359930804 1104 120-121 0.5457384352706036 1105 1 0.6955444648897142 1105 2 0.8040131912137376 1105 3 0.9247371838287428 1105 4 1.0951173331713164 1105 5 1.188578510246188 1105 6 0.9571684425779523 1105 7 1.004525255103836 1105 8 0.9298774010792883 1105 9 1.1826581222322474 1105 10-11 1.2688092476220802 1105 12-13 1.3725570134609413 1105 14-15 1.4386625141450722 1105 16-17 1.3994103291923423 1105 18-19 1.2812028572949785 1105 20-21 1.3792498119684424 1105 22-23 1.392425730764863 1105 24-25 1.5880311366074196 1105 26-27 1.4933491311426579 1105 28-29 1.5683980412152607 1105 30-31 1.360283159469354 1105 32-33 1.3994536671003672 1105 34-35 1.3303847883605044 1105 36-37 1.3763838486687447 1105 38-39 1.3005844981799939 1105 40-41 1.3986720643952637 1105 42-43 1.3629624020555582 1105 44-45 1.4084086027076879 1105 46-47 1.5206735990349571 1105 48-49 1.5044755496011604 1105 50-51 1.3329298466557873 1105 52-53 1.3658281428496792 1105 54-55 1.2627308600836216 1105 56-57 1.275745474484232 1105 58-59 1.3358728158415332 1105 60-61 1.4283074522822758 1105 62-63 1.5322428279321656 1105 64-65 1.4623304892020386 1105 66-67 1.3241184491176696 1105 68-69 1.36410556852627 1105 70-71 1.172933037614488 1105 72-73 1.049147828780903 1105 74-75 0.886572078634309 1105 76-77 1.0917623054156955 1105 78-79 1.35475058773293 1105 80-81 1.4680029481074968 1105 82-83 1.492782894580099 1105 84-85 1.5238562222449659 1105 86-87 1.1429421032941391 1105 88-89 1.1268970001051422 1105 90-91 1.2892423870733225 1105 92-93 1.235345597747191 1105 94-95 1.3443905952158595 1105 96-97 1.1143115710317097 1105 98-99 1.346344980526677 1105 100-101 1.4417208894597913 1105 102-103 1.6593329360363889 1105 104-105 1.652248058209505 1105 106-107 1.6496780732518879 1105 108-109 1.6745719087165867 1105 110-111 1.6460242194544286 1105 112-113 1.609034500241762 1105 114-115 1.501009627401558 1105 116-117 1.5808844755456626 1105 118-119 1.3294307187526542 1105 120-121 1.1216429497289262 1106 1 0.23679472841560312 1106 2 0.41215512577421265 1106 3 0.4875197851242241 1106 4 0.6248490493099297 1106 5 0.7179609216096239 1106 6 0.7202078969971595 1106 7 0.7503586448494346 1106 8 0.5862079215356673 1106 9 0.7460364370978141 1106 10-11 0.8680897494140609 1106 12-13 0.9132819659295706 1106 14-15 0.9671817210723717 1106 16-17 0.8388475345994095 1106 18-19 0.7612530287560446 1106 20-21 1.0047656941177365 1106 22-23 0.7470335172054163 1106 24-25 1.113722251384786 1106 26-27 1.1573365051095905 1106 28-29 1.0606895463010702 1106 30-31 0.7976115775210246 1106 32-33 1.0428235491374735 1106 34-35 0.9119215359578234 1106 36-37 0.8278264232344839 1106 38-39 0.8406156764356751 1106 40-41 0.9721339919154914 1106 42-43 0.7432440894722703 1106 44-45 0.5687139639364034 1106 46-47 0.7665098899830056 1106 48-49 0.9100795122568783 1106 50-51 0.684112666895345 1106 52-53 0.5567085030629713 1106 54-55 0.7294051343478216 1106 56-57 0.756632932972856 1106 58-59 0.8002849258473859 1106 60-61 0.8018018732699588 1106 62-63 0.7429575488268085 1106 64-65 0.7020044327291686 1106 66-67 0.755516422531052 1106 68-69 0.6015376562122086 1106 70-71 0.35664085171317694 1106 72-73 0.47041731760837635 1106 74-75 0.4336574339445711 1106 76-77 0.5575602231259893 1106 78-79 0.6593886433857286 1106 80-81 0.61334719511974 1106 82-83 0.861514508209563 1106 84-85 0.8451895375263732 1106 86-87 0.7505670435777176 1106 88-89 0.5900294864163023 1106 90-91 0.6132575739010875 1106 92-93 0.669688871889548 1106 94-95 0.7915962537211207 1106 96-97 0.8918129394321994 1106 98-99 0.9689232984868603 1106 100-101 0.9084982531177808 1106 102-103 1.0408184290570652 1106 104-105 1.0659428942625553 1106 106-107 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21577.0 3 3292.0 4 2789.0 5 3316.0 6 3855.0 7 4046.0 8 5091.0 9 6157.0 10 7805.0 11 9219.0 12 10128.0 13 11731.0 14 14425.0 15 14517.0 16 11111.0 17 11070.0 18 11607.0 19 12674.0 20 13955.0 21 15404.0 22 17626.0 23 19568.0 24 22227.0 25 25141.0 26 28900.0 27 33600.0 28 39025.0 29 46359.0 30 54601.0 31 66202.0 32 81097.0 33 100426.0 34 129371.0 35 171207.0 36 233183.0 37 269590.0 38 96688.0 39 353.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74888539895775 19.54737170132086 14.53810007188984 22.16564282783155 2 13.822455495529882 20.7902529153828 40.873539830191845 24.51375175889547 3 19.41299034636877 26.28146287260254 30.705806424440173 23.599740356588512 4 11.426389865942712 16.573055277576863 41.14632358040961 30.854231276070827 5 11.44140355744762 37.950030253354036 36.32248031674054 14.286085872457804 6 27.4075959684547 37.90130728548273 19.165881626467034 15.525215119595531 7 25.399541152320708 32.965635962264265 23.864351993758184 17.770470891656835 8 23.472625208510575 34.008059312977004 21.350925619773303 21.16838985873912 9 23.325075401869828 18.913284289352468 23.86463762211202 33.89700268666568 10-11 23.317955287469978 26.107117139596784 29.206419771737874 21.368507801195364 12-13 25.206679116121716 24.082977516680454 28.92427173164655 21.786071635551284 14-15 21.620001715126204 25.864257977271794 28.679386876436304 23.836353431165698 16-17 19.878370699972287 29.44720288219329 29.152295180191267 21.52213123764315 18-19 19.443054173888157 29.84488297156704 30.40304906980576 20.30901378473904 20-21 20.525487920108045 27.823377241162095 29.32615166302721 22.32498317570265 22-23 19.915140353137016 27.554954718401962 29.92087486623598 22.60903006222504 24-25 21.094966718703848 27.532601675304097 29.6380639691963 21.734367636795756 26-27 20.713072637114767 26.795740141840458 31.15269755409583 21.33848966694894 28-29 19.76896935508745 28.535833533074324 30.655441532871176 21.039755578967057 30-31 20.216732725210704 27.801882591080577 29.891382782164133 22.090001901544586 32-33 20.256313539533714 27.761240621202493 30.270521868066936 21.711923971196857 34-35 21.425509668083926 26.97260998622385 30.093893398348843 21.507986947343383 36-37 21.126312538891046 26.81923364857084 30.964442163421523 21.09001164911659 38-39 20.87467415888318 27.672578493758497 30.613719440152543 20.839027907205775 40-41 20.237059887509602 28.220223494140097 30.43175004336084 21.11096657498947 42-43 19.813193868269437 28.4968080549808 29.25833462633973 22.43166345041003 44-45 20.193605626082878 27.769246371829947 29.175364520189706 22.861783481897472 46-47 21.10385890463813 27.97168604820395 29.29587949570225 21.628575551455675 48-49 21.10637823475583 27.800884743364033 29.33185927718604 21.760877744694096 50-51 19.831752592652265 28.235168355854114 30.359423558974903 21.57365549251872 52-53 20.203976905493445 28.974729892570224 28.91474215026171 21.906551051674626 54-55 19.68550003592657 28.25621715506156 29.531083917036877 22.527198891974994 56-57 20.879346971961738 27.533662772394546 29.088985729265044 22.498004526378672 58-59 21.760950261676314 27.197300471810326 29.09628127230196 21.945467994211405 60-61 20.941558642721102 27.47104388244318 30.383818031541633 21.20357944329409 62-63 20.305822047744044 27.91234794459027 29.892695920153006 21.88913408751268 64-65 19.88823165066584 29.140043357076493 29.52579746051409 21.445927531743575 66-67 20.15856940663452 27.86708662782955 28.923462387770332 23.050881577765598 68-69 19.865596560461412 28.41553244082098 29.125370933668698 22.59350006504891 70-71 20.93397012128468 27.922370863914768 28.667014929442708 22.476644085357844 72-73 20.343536877461414 27.22152504717551 30.022340587052376 22.4125974883107 74-75 19.91074662782205 28.797567687577253 29.375186112368645 21.916499572232052 76-77 20.34912908312013 27.717896560956746 29.070042740128123 22.862931615795006 78-79 20.442807653314084 27.693904630192414 29.419405789855897 22.44388192663761 80-81 21.015168720980665 27.61656484225975 28.96094462792162 22.407321808837967 82-83 20.19297579032121 27.121453758391635 30.10943600417265 22.576134447114505 84-85 20.42449998234193 27.612232518976228 29.576243515306448 22.387023983375393 86-87 20.62493011020981 28.057579584530174 29.058447844172953 22.25904246108707 88-89 20.040212878590992 27.485697664945754 28.588810461419705 23.885278995043553 90-91 19.773877932125586 26.29415633438879 29.853459938395122 24.078505795090507 92-93 20.43704522855895 27.145560935113856 29.90911540856248 22.508278427764715 94-95 19.791674410421482 27.013840877609024 29.94661765179618 23.24786706017331 96-97 18.66812754631695 27.430307738820986 30.337662691686244 23.56390202317582 98-99 19.415201815452914 27.905648006881073 29.296518192330428 23.382631985335582 100-101 18.794663963411875 26.691955978751526 30.28660531881077 24.226774739025824 102-103 19.643865514409768 26.64333366064384 29.73229820610092 23.980502618845463 104-105 20.373093739275443 26.816189944037177 29.333061179989173 23.477655136698207 106-107 19.691238681549585 27.017714919695297 30.058155633842514 23.232890764912604 108-109 19.889932456018357 26.85832675403887 29.559652390242963 23.692088399699802 110-111 19.626732395430622 27.214644513975312 28.719311210789 24.439311879805068 112-113 20.30100457729911 26.202582579252446 28.19717853510941 25.299234308339035 114-115 19.830719901965736 26.13504678527962 28.9239803869516 25.11025292580304 116-117 20.52894470070069 25.67900331880067 28.57511543923918 25.21693654125946 118-119 20.430927703460075 25.44859644567985 29.19085029931215 24.92962555154793 120-121 19.765550486750843 27.167347687271377 28.392813056514022 24.67428876946376 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4163.0 1 4256.5 2 2680.5 3 1042.0 4 1002.0 5 922.5 6 915.5 7 1013.5 8 1133.5 9 1170.0 10 1132.0 11 1121.5 12 1202.5 13 1266.5 14 1381.0 15 1559.0 16 1688.0 17 1766.0 18 1922.0 19 2118.0 20 2311.5 21 2624.0 22 3052.5 23 3682.0 24 4450.0 25 5522.0 26 7106.5 27 9391.5 28 12818.0 29 17233.5 30 22044.0 31 26849.5 32 31585.0 33 36094.0 34 40055.5 35 44259.5 36 48265.5 37 52458.5 38 56340.0 39 58027.5 40 59542.0 41 61015.5 42 62457.5 43 65050.0 44 66279.5 45 66827.0 46 69503.5 47 80849.0 48 108158.0 49 105845.0 50 76116.0 51 59118.0 52 46339.5 53 37631.5 54 31475.5 55 26293.5 56 21367.5 57 16781.5 58 13321.5 59 10674.5 60 8465.5 61 6495.0 62 4758.0 63 3282.5 64 2128.5 65 1344.5 66 829.0 67 510.0 68 296.5 69 187.5 70 113.0 71 69.0 72 57.5 73 37.5 74 28.5 75 26.0 76 19.0 77 13.5 78 12.5 79 12.5 80 10.0 81 6.0 82 3.0 83 2.5 84 2.5 85 3.5 86 4.5 87 3.5 88 2.5 89 2.5 90 2.0 91 1.0 92 1.5 93 2.0 94 1.0 95 2.0 96 1.5 97 0.5 98 1.0 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5155522050323739 2 0.9204798478513236 3 0.8846895483116863 4 0.8656586857777452 5 0.8739463194618808 6 0.8819270037503077 7 0.8605633258089804 8 0.8537490492242468 9 0.8545471176530894 10-11 0.8508637249045847 12-13 0.8514162338168605 14-15 0.8527975060975498 16-17 0.8711530799609315 18-19 0.8925474528418296 20-21 0.8872372282960685 22-23 0.868605399976549 24-25 0.8632644804912172 26-27 0.8650140920467569 28-29 0.8804536466509059 30-31 0.8874520928730648 32-33 0.8832161912122845 34-35 0.893161351633247 36-37 0.8992082547287089 38-39 0.8875441776917774 40-41 0.8946347087326488 42-43 0.8888333651537541 44-45 0.8785198654579409 46-47 0.8749285575281488 48-49 0.8803615618321933 50-51 0.9354589783619093 52-53 0.8862549902298008 54-55 0.8919335540504121 56-57 0.9380987431650043 58-59 0.9039659703621942 60-61 0.9171034044985276 62-63 0.8960159810133381 64-65 0.8860401256528045 66-67 0.8900304677970181 68-69 0.9062987857695805 70-71 0.9336786718667988 72-73 0.937914573527579 74-75 0.9407692029076702 76-77 0.9491489214105184 78-79 0.9668599015429118 80-81 0.9888374782756565 82-83 0.9558404182369686 84-85 0.9171647943776694 86-87 0.9076186681711279 88-89 0.9069433795005688 90-91 0.917011319679815 92-93 0.9084474315395416 94-95 0.9042115298787611 96-97 0.9108416368260696 98-99 0.9350292492079171 100-101 0.9255138179409466 102-103 0.9065750402257183 104-105 0.899177559789138 106-107 0.8989933901517129 108-109 0.8994538142452759 110-111 0.8976428128105944 112-113 0.9068205997422853 114-115 0.9087850758748212 116-117 0.9268030054029233 118-119 0.9248999191495291 120-121 0.9092761949079551 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1628933.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.09423875785499 #Duplication Level Percentage of deduplicated Percentage of total 1 82.51992540870408 49.58972099791048 2 10.803875853919022 12.985013901512682 3 2.7476078331164877 4.953462034087645 4 1.188253496607432 2.8562875731993222 5 0.6601543449648324 1.9835736411676002 6 0.4112028635036782 1.4826553834382215 7 0.2929136368060219 1.232169541795689 8 0.20585379470777587 0.9896501670703639 9 0.16535261000065538 0.8943065302152479 >10 0.9113645005707858 9.996371213000797 >50 0.060384070679951815 2.5057434740871876 >100 0.027524250524283166 2.94632456683027 >500 0.0019659144815415944 0.812182333331078 >1k 0.0032075446804099702 4.185642776796421 >5k 2.0693836647806257E-4 0.9607280073724151 >10k+ 2.0693836647806257E-4 1.6261678581846337 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13291 0.815932883672932 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12862 0.7895966255211233 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7882 0.4838750273952336 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7569 0.46465999522386736 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4952 0.3040026815099209 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3789 0.23260625206807153 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3526 0.21646071385379262 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 3384 0.20774335101566488 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3364 0.20651555343282996 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3102 0.19043140509769277 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2925 0.17956539648960393 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2582 0.1585086679439854 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2572 0.15789476915256798 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2500 0.15347469785436232 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2482 0.1523696800298109 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2419 0.148502117643881 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2385 0.14641486175306168 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2114 0.1297782045056488 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2041 0.1252967433283014 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2008 0.12327087731662381 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1954 0.1199558238429696 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1941 0.11915775541412692 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.138987914174494E-5 0.0 12-13 0.0 3.069493957087247E-5 0.0 1.841696374252348E-4 0.0 14-15 0.0 6.138987914174494E-5 0.0 4.2972915399221455E-4 0.0 16-17 0.0 6.138987914174494E-5 0.0 6.445937309883218E-4 0.0 18-19 0.0 6.138987914174494E-5 0.0 9.208481871261741E-4 0.0 20-21 0.0 6.138987914174494E-5 0.0 0.0014119672202601336 0.0 22-23 0.0 6.138987914174494E-5 0.0 0.00236351034695718 0.0 24-25 0.0 6.138987914174494E-5 0.0 0.003468528171508589 0.0 26-27 0.0 6.138987914174494E-5 0.0 0.004512156116918253 0.0 28-29 0.0 6.138987914174494E-5 0.0 0.00871736283812778 0.0 30-31 0.0 6.138987914174494E-5 0.0 0.016513877489129386 0.0 32-33 0.0 6.138987914174494E-5 0.0 0.02949783692760844 0.0 34-35 0.0 6.138987914174494E-5 0.0 0.043157085036646683 0.0 36-37 0.0 6.138987914174494E-5 0.0 0.057890656030665476 0.0 38-39 0.0 6.138987914174494E-5 0.0 0.07999101252169366 0.0 40-41 0.0 6.138987914174494E-5 0.0 0.1390173813164814 0.0 42-43 0.0 6.138987914174494E-5 0.0 0.18014860034145053 0.0 44-45 0.0 6.138987914174494E-5 0.0 0.22548502608762916 0.0 46-47 0.0 6.138987914174494E-5 0.0 0.3395781164725621 0.0 48-49 0.0 6.138987914174494E-5 0.0 0.3907158857976356 0.0 50-51 0.0 6.138987914174494E-5 0.0 0.4563723615397318 0.0 52-53 0.0 6.138987914174494E-5 0.0 0.5311145393948062 0.0 54-55 0.0 6.138987914174494E-5 0.0 0.6278035990430546 0.0 56-57 0.0 6.138987914174494E-5 0.0 0.9903108353750585 0.0 58-59 0.0 6.138987914174494E-5 0.0 1.234427689782207 0.0 60-61 0.0 6.138987914174494E-5 0.0 1.3673674730636558 0.0 62-63 0.0 6.138987914174494E-5 0.0 1.5414077804305026 0.0 64-65 0.0 6.138987914174494E-5 0.0 1.7050731982223946 0.0 66-67 0.0 6.138987914174494E-5 0.0 2.067948773829249 0.0 68-69 0.0 6.138987914174494E-5 0.0 2.445987649584114 0.0 70-71 0.0 6.138987914174494E-5 0.0 2.569534781356876 0.0 72-73 0.0 6.138987914174494E-5 0.0 2.6842417705332267 0.0 74-75 0.0 6.138987914174494E-5 0.0 3.035729523559287 0.0 76-77 0.0 6.138987914174494E-5 0.0 3.372146061256049 0.0 78-79 0.0 6.138987914174494E-5 0.0 3.6401435786493366 0.0 80-81 0.0 6.138987914174494E-5 0.0 3.8652295705225446 0.0 82-83 0.0 6.138987914174494E-5 0.0 4.36325496505995 0.0 84-85 0.0 6.138987914174494E-5 0.0 5.238367692225524 0.0 86-87 0.0 6.138987914174494E-5 0.0 6.058076053465674 0.0 88-89 0.0 6.138987914174494E-5 0.0 7.076135114212801 0.0 90-91 0.0 6.138987914174494E-5 0.0 8.1871691469201 0.0 92-93 0.0 6.138987914174494E-5 0.0 9.238869861436903 0.0 94-95 0.0 6.138987914174494E-5 0.0 10.231114478004926 0.0 96-97 0.0 6.138987914174494E-5 0.0 11.276891069184552 0.0 98-99 0.0 6.138987914174494E-5 0.0 12.363430540114296 0.0 100-101 0.0 6.138987914174494E-5 0.0 13.177767286929543 0.0 102-103 0.0 6.138987914174494E-5 0.0 13.846057511266578 0.0 104-105 0.0 6.138987914174494E-5 0.0 14.50228462435226 0.0 106-107 0.0 6.138987914174494E-5 0.0 15.355910893818223 0.0 108-109 0.0 6.138987914174494E-5 0.0 16.386401405091554 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 2135 0.0 56.8492 2 ACCTGGG 2225 0.0 53.705788 3 TATAACG 265 0.0 52.096092 2 TAACGCA 300 0.0 45.96261 4 GTACCCG 225 0.0 41.019604 1 GTACCTG 3190 0.0 39.420288 1 TACCCGG 300 0.0 32.596237 2 CCTGGGG 1805 0.0 31.830063 4 GTACCGG 200 7.4578566E-11 31.726095 1 ATAACGC 480 0.0 31.11859 3 GTATAAC 640 0.0 26.137978 1 GTATAGG 815 0.0 24.77219 1 TCACGCA 305 1.6370905E-11 24.488277 4 ACCCGGG 380 0.0 24.189348 3 CCGATCG 60 0.0015790398 23.940714 24-25 CTGGGGG 1260 0.0 23.25489 5 TATCACG 330 4.5474735E-11 22.660484 2 CCTGGGA 1565 0.0 22.02681 4 GTACATG 6110 0.0 21.714039 1 TACCGGG 270 6.497976E-8 21.30473 2 >>END_MODULE