##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003846_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1628933 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.647946846187043 31.0 31.0 34.0 30.0 34.0 2 31.87426738852979 31.0 31.0 34.0 30.0 34.0 3 31.90927066981883 31.0 31.0 34.0 30.0 34.0 4 35.66245879971736 37.0 35.0 37.0 33.0 37.0 5 35.537516275991706 37.0 35.0 37.0 33.0 37.0 6 35.570678474805284 37.0 35.0 37.0 33.0 37.0 7 35.50689439037701 37.0 35.0 37.0 33.0 37.0 8 35.49702719510256 37.0 35.0 37.0 33.0 37.0 9 37.06856083092429 39.0 37.0 39.0 33.0 39.0 10-11 36.98312392222394 39.0 37.0 39.0 33.0 39.0 12-13 36.93175133661114 39.0 37.0 39.0 33.0 39.0 14-15 38.018205168659485 40.0 37.0 41.0 33.0 41.0 16-17 38.036002708521465 40.0 37.0 41.0 33.0 41.0 18-19 38.05094377730698 40.0 37.0 41.0 33.0 41.0 20-21 37.99224338877044 40.0 37.0 41.0 33.0 41.0 22-23 37.90788970448754 40.0 37.0 41.0 33.0 41.0 24-25 37.80460184673035 39.5 37.0 41.0 32.0 41.0 26-27 37.573666627172514 39.0 36.5 41.0 32.0 41.0 28-29 37.404065728915796 39.0 36.0 41.0 32.0 41.0 30-31 37.19911684519867 39.0 36.0 40.0 31.0 41.0 32-33 36.96066075154718 39.0 35.5 40.0 31.0 41.0 34-35 36.942102898032026 39.0 36.0 40.0 31.0 41.0 36-37 36.765671147923214 39.0 35.0 40.0 30.5 41.0 38-39 36.52170531261875 38.5 35.0 40.0 30.0 41.0 40-41 36.68239332127227 39.0 35.0 40.0 30.0 41.0 42-43 36.56880761823844 39.0 35.0 40.5 30.0 41.0 44-45 36.41739623422203 39.0 35.0 41.0 30.0 41.0 46-47 36.147733823306424 39.0 35.0 40.0 29.5 41.0 48-49 35.89743654281668 39.0 35.0 40.0 28.5 41.0 50-51 35.65797273429908 39.0 35.0 40.0 28.0 41.0 52-53 35.47145125060392 38.0 34.5 40.0 27.5 41.0 54-55 35.2730321013817 38.0 34.0 40.0 27.0 41.0 56-57 35.03779805553697 38.0 34.0 40.0 26.5 41.0 58-59 34.76551920797233 38.0 34.0 40.0 26.0 41.0 60-61 34.3248043351077 37.5 33.0 40.0 25.0 41.0 62-63 34.10744057613174 37.0 33.0 40.0 25.0 41.0 64-65 33.661136461720645 36.5 32.5 39.5 23.5 41.0 66-67 33.30845498249468 36.0 32.0 39.0 23.0 41.0 68-69 33.022979766509735 36.0 32.0 39.0 22.5 40.0 70-71 32.60243453843712 35.0 31.5 38.5 22.0 40.0 72-73 32.220710121287986 35.0 31.0 38.0 21.5 40.0 74-75 31.827010073465267 35.0 31.0 37.0 20.0 39.0 76-77 30.84412679956757 34.0 29.5 36.0 20.0 39.0 78-79 31.220361733723855 34.0 31.0 36.0 20.0 39.0 80-81 31.021677994122534 34.0 31.0 36.0 19.5 38.5 82-83 30.71397749324251 34.0 31.0 35.5 18.0 37.0 84-85 30.36842000254154 34.0 30.0 35.0 17.5 37.0 86-87 30.027137703024003 34.0 30.0 35.0 12.5 36.0 88-89 29.710039332495565 34.0 30.0 35.0 8.5 36.0 90-91 29.413177521727413 34.0 29.5 35.0 6.0 36.0 92-93 29.162148780827696 34.0 29.0 35.0 2.0 35.0 94-95 28.82671386729841 34.0 29.0 35.0 2.0 35.0 96-97 28.42448277492076 34.0 29.0 35.0 2.0 35.0 98-99 28.02147571447076 34.0 29.0 35.0 2.0 35.0 100-101 27.01180159036621 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.04643606466800776 1101 2 0.08569148780069469 1101 3 0.07039527617618901 1101 4 0.15492912686890747 1101 5 0.14761057952486567 1101 6 0.07522138133879253 1101 7 -0.058574032264132825 1101 8 0.08759583706443408 1101 9 0.16866469886542035 1101 10-11 0.21666944880907835 1101 12-13 0.19753824175251822 1101 14-15 0.21366902573029023 1101 16-17 0.28947845712837506 1101 18-19 0.07494111187705244 1101 20-21 0.10102512035393119 1101 22-23 0.11559066794877992 1101 24-25 0.03078189838721812 1101 26-27 0.13472103181534578 1101 28-29 0.25509373273340685 1101 30-31 0.28023923579809207 1101 32-33 0.2935138332812386 1101 34-35 0.4064684164479502 1101 36-37 0.31851854035621585 1101 38-39 0.1899091766016241 1101 40-41 0.2621574718643487 1101 42-43 0.13130923407847916 1101 44-45 0.3979699300049191 1101 46-47 0.4374274500376316 1101 48-49 0.4923225228172896 1101 50-51 0.5747169756063926 1101 52-53 0.5346223945684017 1101 54-55 0.5832560754802287 1101 56-57 0.6816260053619771 1101 58-59 0.528211005307945 1101 60-61 0.589855498374682 1101 62-63 0.30916125291787466 1101 64-65 0.286270682022824 1101 66-67 0.2899433603559558 1101 68-69 0.1904260084162459 1101 70-71 0.25724098397284934 1101 72-73 0.09268604749956211 1101 74-75 0.08942878744762695 1101 76-77 0.10428553429899878 1101 78-79 0.19292125902502733 1101 80-81 0.19786948921582237 1101 82-83 0.20314599873237427 1101 84-85 -0.05773134583894546 1101 86-87 -0.05714447887004681 1101 88-89 -0.06768666158816927 1101 90-91 -0.13104452035408443 1101 92-93 0.16287378687850662 1101 94-95 0.17038370106812906 1101 96-97 0.13275246635918947 1101 98-99 -0.03562659159651105 1101 100-101 0.09035118121749974 1102 1 -0.05680419031027384 1102 2 -0.04500406136159896 1102 3 -0.10219568676170354 1102 4 -0.12108147842245387 1102 5 -0.28180161656020175 1102 6 -0.07928981626937315 1102 7 -0.14851310824991515 1102 8 -0.18565908480616145 1102 9 -0.061437969380754964 1102 10-11 -0.13389127776620313 1102 12-13 -0.04262281200728779 1102 14-15 -0.07460112714385758 1102 16-17 -0.18121624586705565 1102 18-19 -0.16351096687287736 1102 20-21 -0.1417802048562038 1102 22-23 -0.2862934636019645 1102 24-25 -0.22371688877360896 1102 26-27 -0.22144752462398287 1102 28-29 -0.20181409148952412 1102 30-31 -0.21572426620450358 1102 32-33 -0.1349768870888255 1102 34-35 -0.3161366492710087 1102 36-37 -0.2859328614602177 1102 38-39 -0.34350792725125956 1102 40-41 -0.23682797406558365 1102 42-43 -0.37511119803073 1102 44-45 -0.42147921536655986 1102 46-47 -0.43097075608861957 1102 48-49 -0.4243689647440618 1102 50-51 -0.25397767097791757 1102 52-53 -0.24027465512979518 1102 54-55 -0.3944347351476267 1102 56-57 -0.2489155414310531 1102 58-59 -0.37048223099825606 1102 60-61 -0.4006561505224866 1102 62-63 -0.48791918934534095 1102 64-65 -0.3747678467750575 1102 66-67 -0.40066136689625864 1102 68-69 -0.40908744563689226 1102 70-71 -0.452070800311013 1102 72-73 -0.40691117707312685 1102 74-75 -0.45030720849077355 1102 76-77 -0.35489227356471176 1102 78-79 -0.416499398728682 1102 80-81 -0.32490791248779516 1102 82-83 -0.38210305638129327 1102 84-85 -0.38194300037721973 1102 86-87 -0.501361210409506 1102 88-89 -0.4524172984697614 1102 90-91 -0.21970842287566228 1102 92-93 -0.19260514356657765 1102 94-95 -0.19058036504570808 1102 96-97 -0.34269120713322465 1102 98-99 -0.7154457939432142 1102 100-101 -0.7034097484378279 1103 1 0.01880576481008589 1103 2 -0.038741894316078884 1103 3 -0.1403830155487853 1103 4 -0.034094242891768545 1103 5 0.12082063674748866 1103 6 0.008943557863631213 1103 7 0.13439474277299013 1103 8 0.02895244401589281 1103 9 0.09719873866843898 1103 10-11 -0.015645663731113757 1103 12-13 -0.005423126747622575 1103 14-15 0.05584213216721423 1103 16-17 -0.07959853880871037 1103 18-19 -0.032958422721804936 1103 20-21 0.11644442648960762 1103 22-23 -0.059173337705829 1103 24-25 -0.02882559076113722 1103 26-27 -0.08858338753707784 1103 28-29 -0.0425173030066901 1103 30-31 0.1466785431444464 1103 32-33 -0.09895339813387238 1103 34-35 0.02742108226713924 1103 36-37 0.15161507815626152 1103 38-39 0.09013635788017638 1103 40-41 0.176435708396383 1103 42-43 0.28774740195760984 1103 44-45 0.19090202844063953 1103 46-47 0.21106427334543554 1103 48-49 0.22593644995548345 1103 50-51 0.31744040910601257 1103 52-53 0.26448142110730544 1103 54-55 0.06296020569284622 1103 56-57 0.12120219476991423 1103 58-59 0.03549113264340775 1103 60-61 0.08044777207471299 1103 62-63 -0.05595876502873409 1103 64-65 -0.04712140508944174 1103 66-67 -0.23889940384911057 1103 68-69 -0.1720661132361201 1103 70-71 -0.031119002211106306 1103 72-73 0.09055399749119886 1103 74-75 0.15403212822310763 1103 76-77 0.07173505055955687 1103 78-79 -0.00371161531666786 1103 80-81 -0.036030194938106774 1103 82-83 0.04694106555144373 1103 84-85 -0.055367841549919916 1103 86-87 -0.006908217462019195 1103 88-89 0.014967442452313406 1103 90-91 -0.06699845026972895 1103 92-93 -0.09392384730266201 1103 94-95 -0.32619789724830994 1103 96-97 -0.28255307109545313 1103 98-99 -0.14935877406794518 1103 100-101 -0.027723439642581837 1104 1 -0.054871405162863596 1104 2 -0.10771328406451985 1104 3 -0.08462470432466418 1104 4 -0.007545106190846695 1104 5 -0.04908780972411364 1104 6 0.02979192455519808 1104 7 -0.0025901216871559996 1104 8 -0.09013405830413745 1104 9 -0.2329226555108903 1104 10-11 -0.15332485081150793 1104 12-13 -0.10654811928746 1104 14-15 0.028747725390310563 1104 16-17 -0.13461008020794196 1104 18-19 -0.2020016410269605 1104 20-21 -0.157747600209035 1104 22-23 -0.1545698295304021 1104 24-25 -0.08536201956465561 1104 26-27 -0.1314996691387833 1104 28-29 -0.1079082667753255 1104 30-31 -0.2555674028976327 1104 32-33 -0.07167015127814125 1104 34-35 0.09204975304398033 1104 36-37 -0.12500715306838828 1104 38-39 -0.22666224063406304 1104 40-41 -0.08557063228980866 1104 42-43 -0.17236258043339348 1104 44-45 0.15941999100539306 1104 46-47 -0.023750603093169786 1104 48-49 -0.06938773103922102 1104 50-51 -0.07210849067823233 1104 52-53 0.07116418966864302 1104 54-55 -0.02745410311026575 1104 56-57 -0.07134656455227173 1104 58-59 0.0610570349674191 1104 60-61 0.06080704748342214 1104 62-63 0.06566374048877321 1104 64-65 -0.022893509325548678 1104 66-67 -0.26508849350428676 1104 68-69 -0.24628840257469164 1104 70-71 -0.1894979555698697 1104 72-73 0.0750471073150365 1104 74-75 -0.013873377587980684 1104 76-77 -0.19105377830642212 1104 78-79 -0.20469668835994526 1104 80-81 -0.10269993609608363 1104 82-83 -0.0311678932148034 1104 84-85 -0.08924753007380914 1104 86-87 -0.07731256129624953 1104 88-89 -0.18551531296708035 1104 90-91 -0.18637160369106454 1104 92-93 -0.11878126023276536 1104 94-95 -0.17411788331061828 1104 96-97 -0.12418105041317418 1104 98-99 -0.20429933088846042 1104 100-101 -4.902286257788546E-4 1105 1 0.0496643469635103 1105 2 0.08623618436312341 1105 3 0.18953268780246546 1105 4 0.17660406034559628 1105 5 0.04531277825965674 1105 6 0.2380072117897356 1105 7 0.1198166277620416 1105 8 0.2410900435351948 1105 9 0.25333373907017886 1105 10-11 0.2601166916842388 1105 12-13 0.20712233160885063 1105 14-15 0.2937012138822084 1105 16-17 0.3182018182206079 1105 18-19 0.22637115167269428 1105 20-21 0.1729450151338341 1105 22-23 0.29091888904621044 1105 24-25 0.23703356780949036 1105 26-27 0.2360736442287461 1105 28-29 0.16381682333697256 1105 30-31 0.2546484716337787 1105 32-33 0.1653211371515937 1105 34-35 0.21679212376568557 1105 36-37 0.18069340452019134 1105 38-39 0.2156262251644776 1105 40-41 0.3434866531965781 1105 42-43 0.2531197500018081 1105 44-45 0.3757190247131419 1105 46-47 0.43358262262631087 1105 48-49 0.4188503611732557 1105 50-51 0.482910922746143 1105 52-53 0.4480949281343598 1105 54-55 0.455229053661931 1105 56-57 0.40500941291062986 1105 58-59 0.1652831683044198 1105 60-61 0.3687693849249669 1105 62-63 0.40492573843151547 1105 64-65 0.34760804563736514 1105 66-67 0.4493303315928685 1105 68-69 0.5210760941273449 1105 70-71 0.6092740444507356 1105 72-73 0.35699219334028953 1105 74-75 0.3021500387282181 1105 76-77 0.3305061989572877 1105 78-79 0.4673541626194826 1105 80-81 0.6135654396622954 1105 82-83 0.5688997661324038 1105 84-85 0.40191056097094346 1105 86-87 0.6635516237263062 1105 88-89 0.5882733872350414 1105 90-91 0.6990289173202555 1105 92-93 0.8008159090650011 1105 94-95 0.9073425969333719 1105 96-97 0.9305048745536411 1105 98-99 0.7153934888062459 1105 100-101 0.7002595193689132 1106 1 -0.11595485667575645 1106 2 -0.11450033423261985 1106 3 -0.04631650382582109 1106 4 -0.008400204422002844 1106 5 -0.03862403741921838 1106 6 -0.08591668386755202 1106 7 -0.035910249170889585 1106 8 0.04509795801548222 1106 9 0.10478907892670719 1106 10-11 -0.0972418215075308 1106 12-13 -0.10694095715555818 1106 14-15 -0.03008032944144645 1106 16-17 -0.03613944442138006 1106 18-19 -0.05122015400379354 1106 20-21 -0.07512848973148323 1106 22-23 -0.08849263104848148 1106 24-25 -0.17400570093276713 1106 26-27 -0.0880985943584065 1106 28-29 -0.15931562429149437 1106 30-31 -0.03272820308533397 1106 32-33 0.04203705334165875 1106 34-35 0.06540486546002455 1106 36-37 -0.1700927246374775 1106 38-39 0.06667617033175333 1106 40-41 -0.29461928711214824 1106 42-43 -0.11727115801652843 1106 44-45 -0.006707038142380384 1106 46-47 -0.046256341296270875 1106 48-49 -0.18422472001205392 1106 50-51 0.023334923813791875 1106 52-53 0.08417756985326008 1106 54-55 -0.09737206774951801 1106 56-57 0.015015853580081284 1106 58-59 -0.011388843021876482 1106 60-61 0.20064915135883155 1106 62-63 0.060917978682020646 1106 64-65 -0.09437448138733373 1106 66-67 -0.11018087381807362 1106 68-69 -0.038245372643253006 1106 70-71 0.010788278717029698 1106 72-73 -0.21880346143209195 1106 74-75 -0.024961117942513766 1106 76-77 -0.12540229324911323 1106 78-79 0.07287263650962572 1106 80-81 0.1987190196494133 1106 82-83 0.19834387640130657 1106 84-85 0.10608016913453966 1106 86-87 0.20456379523766088 1106 88-89 0.2168956835561211 1106 90-91 0.20682889681842198 1106 92-93 0.14729046455243378 1106 94-95 0.23415647705955323 1106 96-97 0.43349001846295465 1106 98-99 0.2932455158764 1106 100-101 0.11641341462003396 1107 1 -0.08389525548890475 1107 2 -0.13226814377591722 1107 3 -0.14956542855659194 1107 4 -0.27959960560993835 1107 5 -0.15285028591237904 1107 6 -0.09066933155696688 1107 7 -0.19779990743105458 1107 8 -0.12728822038035048 1107 9 -0.2538192676703943 1107 10-11 -0.08758209200380662 1107 12-13 -0.11240654046267906 1107 14-15 -0.10175988036316852 1107 16-17 -0.08030856575083334 1107 18-19 -0.19891610456025433 1107 20-21 -0.19029172833239727 1107 22-23 -0.19329339756485808 1107 24-25 -0.25630620396874804 1107 26-27 -0.14114969724499815 1107 28-29 -0.29603617430007034 1107 30-31 -0.4615140700742799 1107 32-33 -0.24409033884870723 1107 34-35 -0.22145065574265033 1107 36-37 -0.30873283504537596 1107 38-39 -0.15329033496439592 1107 40-41 -0.17411682039722365 1107 42-43 -0.23877196538197865 1107 44-45 -0.27415867122074644 1107 46-47 -0.2669936709667766 1107 48-49 -0.3839568931598336 1107 50-51 -0.29626255508277666 1107 52-53 -0.31673572625177115 1107 54-55 -0.48142377298689354 1107 56-57 -0.6005374129228329 1107 58-59 -0.550435674833686 1107 60-61 -0.4573358044880109 1107 62-63 -0.6797830868557426 1107 64-65 -0.7006893946849786 1107 66-67 -0.5819563546793987 1107 68-69 -0.6203616317294731 1107 70-71 -0.43470436037176086 1107 72-73 -0.3982939942163597 1107 74-75 -0.4965265837796835 1107 76-77 -0.5898784057710103 1107 78-79 -0.6617249154690903 1107 80-81 -0.7612167680646316 1107 82-83 -0.6141152072100624 1107 84-85 -0.648686710342222 1107 86-87 -0.4325558217810972 1107 88-89 -0.6972941392542822 1107 90-91 -0.507336039596261 1107 92-93 -0.5888327734945946 1107 94-95 -0.6833466597033073 1107 96-97 -0.5019753648298497 1107 98-99 -0.4231724663330958 1107 100-101 -0.3577880090202754 1108 1 -0.18138840419105406 1108 2 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-1.0168152597096238 2316 74-75 -0.9893218010469305 2316 76-77 -0.8980321325579901 2316 78-79 -0.7909214351280944 2316 80-81 -0.9189930084051596 2316 82-83 -0.8193845779422055 2316 84-85 -0.7904771863093423 2316 86-87 -0.7145830500553494 2316 88-89 -0.7385191184402444 2316 90-91 -0.8123359027904016 2316 92-93 -0.7093865831468378 2316 94-95 -1.0014354232519729 2316 96-97 -0.5213016350722306 2316 98-99 -0.8340947527889249 2316 100-101 -1.1818488368129678 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 9.0 5 35.0 6 76.0 7 215.0 8 508.0 9 968.0 10 1683.0 11 2748.0 12 3984.0 13 5674.0 14 6646.0 15 8752.0 16 11310.0 17 14563.0 18 12710.0 19 9226.0 20 8385.0 21 8589.0 22 9177.0 23 10565.0 24 12173.0 25 14950.0 26 18612.0 27 23602.0 28 29517.0 29 37495.0 30 46228.0 31 57282.0 32 70519.0 33 89344.0 34 116177.0 35 154444.0 36 218056.0 37 295872.0 38 275026.0 39 53776.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16365380811807 20.7545106884305 14.763129736576042 20.318705766875386 2 15.108415849790068 22.71640348782313 39.7699136884718 22.405266973914998 3 20.943256940571853 27.317147468573662 30.083549720804886 21.6560458700496 4 12.932269160241706 17.61693083754826 39.70451823371495 29.74628176849508 5 12.383136691318795 39.380256892088255 34.5736749148062 13.662931501786751 6 28.334371863306945 39.15077920443488 18.135987058791027 14.378861873467145 7 27.130782623215705 33.626265553135426 22.345438606399775 16.897513217249095 8 25.026136741044596 34.65022809409595 19.964541205807727 20.359093959051723 9 24.852280603315176 19.781722145723613 22.593010271140678 32.77298697982054 10-11 24.62145326340591 27.105757348242125 28.176032443442832 20.09675694490913 12-13 26.772525327929387 24.70147636520348 28.07567898741078 20.450319319456355 14-15 22.401585135821644 26.99273888693629 27.989373082466045 22.61630289477602 16-17 20.525399141646712 30.054827301061493 28.581807845994895 20.837965711296906 18-19 20.329964461398966 30.40935385310507 29.99245518385348 19.268226501642484 20-21 21.786090015174338 27.939264577173777 29.330926150423963 20.94371925722792 22-23 20.9471476113505 28.10391833181598 29.570399764753986 21.37853429207954 24-25 22.141733269569713 28.256349401724933 29.538262163023283 20.063655165682075 26-27 21.924911914057343 27.23594630916984 30.950505492670406 19.88863628410241 28-29 20.828601995066823 29.30688388612862 30.235745740260654 19.6287683785439 30-31 21.461447098720466 28.333043234543183 29.487145246785047 20.718364419951307 32-33 21.459325223242377 28.426666912240954 29.766274668704934 20.347733195811728 34-35 22.49547522979939 27.598488960051863 29.98056252041365 19.925473289735095 36-37 22.362532774151557 27.37628717218173 30.54759697157629 19.713583082090423 38-39 21.62006679928289 28.298490876495002 30.63081730887301 19.4506250153491 40-41 21.167171520415646 28.798460357238138 29.80991772833312 20.2244503940131 42-43 20.78827060413166 29.202735778574073 28.877614978639393 21.131378638654873 44-45 21.657397818080916 27.95722721560678 28.922675149929432 21.462699816382873 46-47 22.468187745099243 28.730634550736312 28.585846431895256 20.215331272269193 48-49 22.397913235228213 28.523855800085084 28.883661881734852 20.194569082951848 50-51 21.062069465103843 28.944898286178745 29.716108642896916 20.276923605820496 52-53 21.662094143835258 29.99669108551426 28.004129083271074 20.337085687379407 54-55 20.959701841634985 29.06988808011134 28.571310176661658 21.399099901592024 56-57 22.213265991757062 28.318915200629625 28.360139099194893 21.107679708418424 58-59 23.3066399169761 27.97430949240559 28.11937417756519 20.59967641305312 60-61 21.868879553918866 28.554049026192903 29.610127200904735 19.9669442189835 62-63 21.295759047122587 28.601089111524946 29.20342218513668 20.899729656215786 64-65 21.060234304226995 30.006909364492007 28.569485175574627 20.363371155706368 66-67 21.62009480267761 28.288028452470172 28.28274765042687 21.809129094425344 68-69 21.304090384617155 29.17465132262123 28.176496426588283 21.34476186617333 70-71 22.145385755114482 28.97564257731386 27.87470413833388 21.004267529237776 72-73 21.768513499327476 27.94562452844899 29.1380001510191 21.147861821204433 74-75 21.04982864544725 29.84439195608167 28.406886104857016 20.69889329361407 76-77 21.814187569408933 28.48665353332519 27.793500407935745 21.905658489330133 78-79 21.6567839192895 28.9907872208372 28.14814973973761 21.2042791201357 80-81 22.193154105526165 28.71266918672198 27.87159688937387 21.222579818377987 82-83 21.451526858379076 28.189802772735284 28.834918317696307 21.523752051189337 84-85 21.129777749947664 28.59498038897903 28.736115894411736 21.539125966661572 86-87 21.58188472881485 28.809938339664708 27.718420257789806 21.889756673730634 88-89 21.093102049009996 28.873808806132605 27.528510994620405 22.504578150236995 90-91 21.47028146645688 27.06161026880786 28.67972470322598 22.78838356150928 92-93 21.44793555044928 28.382935332515213 28.563789916466792 21.605339200568714 94-95 21.00235645268079 28.407118408600972 28.598900567642037 21.991624571076194 96-97 20.17183085189488 28.603053235948195 29.172630457770527 22.052485454386396 98-99 20.688244258875855 29.369201514493433 27.722903566917495 22.219650659713217 100-101 19.76748362025701 28.51611576399664 28.149737479031323 23.56666313671503 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 209.0 1 155.0 2 244.5 3 387.5 4 315.0 5 256.5 6 355.5 7 575.5 8 903.0 9 1148.5 10 1169.5 11 1125.5 12 1239.0 13 1416.5 14 1560.0 15 1730.0 16 1897.0 17 2092.5 18 2298.0 19 2450.5 20 2625.0 21 2888.0 22 3246.5 23 3851.5 24 4593.5 25 5576.0 26 7100.0 27 9240.5 28 12478.5 29 16926.5 30 21795.0 31 26524.0 32 31276.5 33 35752.0 34 39719.5 35 43881.5 36 47856.0 37 51532.0 38 55161.5 39 57485.5 40 58974.5 41 60108.0 42 61677.0 43 64800.0 44 66139.0 45 66909.5 46 70037.5 47 82923.5 48 114217.5 49 110518.0 50 77166.0 51 60508.0 52 47489.5 53 38132.0 54 31657.0 55 26460.0 56 21504.5 57 16823.0 58 13281.5 59 10694.5 60 8532.5 61 6489.5 62 4711.5 63 3229.0 64 2023.5 65 1248.0 66 775.0 67 435.0 68 220.5 69 126.0 70 77.0 71 39.0 72 25.0 73 18.0 74 14.5 75 6.5 76 1.5 77 1.5 78 2.0 79 1.0 80 1.5 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05592617989812963 2 0.004051732023355166 3 0.0418065076955283 4 0.0 5 0.0 6 0.0017189166159688582 7 6.138987914174494E-5 8 0.0 9 0.0 10-11 3.6833927485046956E-4 12-13 0.0 14-15 0.003100188896658119 16-17 0.0 18-19 0.0 20-21 0.012063111251352879 22-23 0.0 24-25 0.0 26-27 0.007735124771859862 28-29 0.010160024997958787 30-31 0.010896703547659726 32-33 0.0060162081558910036 34-35 0.007428175376151137 36-37 0.02228452612845341 38-39 0.010620449091521872 40-41 0.014948435571014891 42-43 0.0 44-45 0.0 46-47 6.138987914174494E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.001442662159831006 58-59 2.762544561378522E-4 60-61 0.010497669333238384 62-63 0.01632970785170415 64-65 0.043464034432355414 66-67 0.024095527563134884 68-69 0.0016882216763979855 70-71 2.762544561378522E-4 72-73 0.0 74-75 3.069493957087247E-5 76-77 0.0 78-79 0.0 80-81 3.069493957087247E-5 82-83 0.0 84-85 1.2277975828348987E-4 86-87 5.525089122757044E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 9.208481871261739E-5 96-97 3.376443352795971E-4 98-99 3.376443352795971E-4 100-101 9.515431266970465E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1628933.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.54420173696052 #Duplication Level Percentage of deduplicated Percentage of total 1 80.40221269261669 45.46278934589326 2 12.065784595162016 13.64500316527103 3 3.1019929762384475 5.26199149905184 4 1.3638126573542237 3.084627921154298 5 0.7088550088090334 2.0040820310176457 6 0.45545689222412733 1.5452067837846082 7 0.3302060442234266 1.3069866025533199 8 0.23929531034084214 1.0824609842096922 9 0.18187835962038404 0.9255749992166205 >10 1.0340740007930445 10.840734770293734 >50 0.07485883763952342 2.9098100136025 >100 0.035151939950200474 3.575662953626752 >500 0.0027206292491408937 1.016423957983955 >1k 0.003155929929003437 4.4163799423941414 >5k 4.3530067986254306E-4 1.5322046587843854 >10k+ 1.0882516996563577E-4 1.3900603711622197 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 22590 1.386797369812018 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7008 0.4302202730253485 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6463 0.3967627888930975 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 5774 0.35446516216443524 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 5655 0.3471597665465676 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4949 0.30381851187249564 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4853 0.29792508347488816 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4540 0.278710051303522 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4443 0.27275523302677274 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4106 0.2520668437560047 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4031 0.2474626028203738 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3819 0.2344479484423239 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3751 0.23027343666068523 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3523 0.21627654421636738 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 3444 0.21142674376416953 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2883 0.17698702156565066 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2486 0.1526152395463779 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2308 0.1416878410591473 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2216 0.13603997217810676 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1959 0.12026277323867833 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1898 0.11651799061103188 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1722 0.10571337188208477 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1703 0.10454696417839163 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1651 0.10135469046302088 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 6.138987914174494E-5 0.0 12-13 0.0 0.0 0.0 1.841696374252348E-4 0.0 14-15 0.0 0.0 0.0 3.9903421442134205E-4 0.0 16-17 0.0 0.0 0.0 5.525089122757044E-4 0.0 18-19 0.0 0.0 0.0 7.673734892718117E-4 0.0 20-21 0.0 0.0 0.0 0.0011664077036931536 0.0 22-23 0.0 0.0 0.0 0.002025866011677583 0.0 24-25 0.0 0.0 0.0 0.003161578775799864 0.0 26-27 0.0 0.0 0.0 0.004205206721209527 0.0 28-29 0.0 3.069493957087247E-5 0.0 0.008625278019415162 0.0 30-31 0.0 6.138987914174494E-5 0.0 0.016728742066125492 0.0 32-33 0.0 6.138987914174494E-5 0.0 0.030265210416880253 0.0 34-35 0.0 6.138987914174494E-5 0.0 0.04450766237776508 0.0 36-37 0.0 9.20848187126174E-5 0.0 0.06006999674019742 0.0 38-39 0.0 1.2277975828348987E-4 0.0 0.08352093057234398 0.0 40-41 0.0 1.2277975828348987E-4 0.0 0.14540192874722288 0.0 42-43 0.0 1.2277975828348987E-4 0.0 0.18815997956944822 0.0 44-45 0.0 1.2277975828348987E-4 0.0 0.23518462699202486 0.0 46-47 0.0 1.2277975828348987E-4 0.0 0.35375917855430516 0.0 48-49 0.0 1.2277975828348987E-4 0.0 0.40646238979749316 0.0 50-51 0.0 1.2277975828348987E-4 0.0 0.47463585058440094 0.0 52-53 0.0 1.2277975828348987E-4 0.0 0.5519870983029995 0.0 54-55 0.0 1.2277975828348987E-4 0.0 0.6516535670896224 0.0 56-57 0.0 1.2277975828348987E-4 0.0 1.0209136901272182 0.0 58-59 0.0 1.2277975828348987E-4 0.0 1.2698803449865648 0.0 60-61 0.0 1.2277975828348987E-4 0.0 1.4057361475272463 0.0 62-63 0.0 1.2277975828348987E-4 0.0 1.5833984577634563 0.0 64-65 0.0 1.2277975828348987E-4 0.0 1.7513918620348412 0.0 66-67 0.0 1.2277975828348987E-4 0.0 2.1210817142264293 0.0 68-69 0.0 1.2277975828348987E-4 0.0 2.5097717340123875 0.0 70-71 0.0 1.2277975828348987E-4 0.0 2.638444920693485 0.0 72-73 0.0 1.2277975828348987E-4 0.0 2.7581858799594583 0.0 74-75 0.0 1.2277975828348987E-4 0.0 3.120386166895753 0.0 76-77 0.0 1.2277975828348987E-4 0.0 3.4684053917503053 0.0 78-79 0.0 1.2277975828348987E-4 0.0 3.746900578476831 0.0 80-81 0.0 1.2277975828348987E-4 0.0 3.9833743929308327 0.0 82-83 0.0 1.2277975828348987E-4 0.0 4.493309424021737 0.0 84-85 0.0 1.2277975828348987E-4 0.0 5.39408312066856 0.0 86-87 0.0 1.2277975828348987E-4 0.0 6.235216549729179 0.0 88-89 0.0 1.2277975828348987E-4 0.0 7.281392175123225 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCA 355 0.0 58.864017 4 TATAACG 350 0.0 54.298874 2 ATAACGC 415 0.0 53.808224 3 TACCTGG 2285 0.0 49.694756 2 ACCTGGG 2330 0.0 47.10369 3 GTACCTG 2775 0.0 41.792576 1 GTATAAC 600 0.0 37.23222 1 CCTGGGG 1770 0.0 33.53994 4 GTACCGG 150 9.495125E-10 31.686995 1 AGTACGC 270 0.0 29.91466 2 TCACGCA 255 0.0 29.79925 4 GTACACG 225 0.0 29.574526 1 TACGCAG 310 0.0 27.57632 4 CTGGGGG 1030 0.0 26.283096 5 GTACGCA 295 0.0 25.768957 3 GTACCCG 280 0.0 25.462763 1 ACACGGG 265 1.8189894E-12 25.100424 3 TACACGG 255 1.8189894E-11 24.221558 2 TATCACG 315 0.0 24.132835 2 TACCGGG 165 2.4258024E-6 23.035887 2 >>END_MODULE