##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003845_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3096577 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.678862498817242 31.0 31.0 34.0 30.0 34.0 2 31.907101938689074 31.0 31.0 34.0 30.0 34.0 3 31.94537581335778 31.0 31.0 34.0 30.0 34.0 4 35.671399419423444 37.0 35.0 37.0 33.0 37.0 5 35.55603913611707 37.0 35.0 37.0 33.0 37.0 6 35.6024006507831 37.0 35.0 37.0 33.0 37.0 7 35.54130383323263 37.0 35.0 37.0 33.0 37.0 8 35.54404363269507 37.0 35.0 37.0 33.0 37.0 9 37.10603708546566 39.0 37.0 39.0 33.0 39.0 10-11 37.026028094893164 39.0 37.0 39.0 33.0 39.0 12-13 36.986330712913 39.0 37.0 39.0 33.0 39.0 14-15 38.036172522110704 40.0 37.0 41.0 33.0 41.0 16-17 38.077035061618034 40.0 37.0 41.0 33.0 41.0 18-19 38.11648507367974 40.0 37.0 41.0 33.0 41.0 20-21 38.05156419491587 40.0 37.0 41.0 33.0 41.0 22-23 37.97041136067341 40.0 37.0 41.0 33.0 41.0 24-25 37.84937593994918 39.5 37.0 41.0 32.5 41.0 26-27 37.597080744318646 39.0 36.5 41.0 32.0 41.0 28-29 37.45732820465953 39.0 36.0 41.0 32.0 41.0 30-31 37.29488108966772 39.0 36.0 40.0 31.5 41.0 32-33 37.07563997278285 39.0 36.0 40.0 31.0 41.0 34-35 37.075972436661516 39.0 36.0 40.0 31.0 41.0 36-37 36.893251160878606 39.0 35.5 40.0 30.5 41.0 38-39 36.64767418992003 39.0 35.0 40.0 30.0 41.0 40-41 36.8628647051244 39.0 35.0 40.5 30.5 41.0 42-43 36.77496280570449 39.0 35.0 41.0 30.0 41.0 44-45 36.64539796685179 39.0 35.0 41.0 30.0 41.0 46-47 36.387889918448664 39.0 35.0 41.0 30.0 41.0 48-49 36.130718370639585 39.0 35.0 40.0 29.0 41.0 50-51 35.88734738390164 39.0 35.0 40.0 28.0 41.0 52-53 35.73120771742475 39.0 35.0 40.0 28.0 41.0 54-55 35.54298875823207 38.0 34.5 40.0 28.0 41.0 56-57 35.30111426261966 38.0 34.0 40.0 27.5 41.0 58-59 35.04352838634402 38.0 34.0 40.0 27.0 41.0 60-61 34.54554852018858 38.0 33.5 40.0 26.0 41.0 62-63 34.35982425110049 37.0 33.0 40.0 26.0 41.0 64-65 33.91337095767359 37.0 33.0 39.5 25.5 41.0 66-67 33.55338636823822 36.0 32.0 39.0 24.0 41.0 68-69 33.25247087348385 36.0 32.0 39.0 24.0 40.0 70-71 32.78828138295931 35.0 31.5 38.5 22.5 40.0 72-73 32.38033028082299 35.0 31.0 37.5 22.5 40.0 74-75 32.01998367875238 35.0 31.0 37.0 22.0 39.0 76-77 31.03075153629314 34.0 30.0 36.0 21.0 39.0 78-79 31.408055249393122 34.0 31.0 36.0 21.0 39.0 80-81 31.207872919032855 34.0 31.0 36.0 20.0 37.5 82-83 30.887516280073125 34.0 31.0 35.5 20.0 37.0 84-85 30.546137557696774 34.0 30.5 35.0 18.5 37.0 86-87 30.272206536443306 34.0 30.0 35.0 18.0 36.0 88-89 29.938847960183132 34.0 30.0 35.0 15.0 36.0 90-91 29.60653069502228 34.0 29.5 35.0 9.0 36.0 92-93 29.369578085737896 34.0 29.0 35.0 6.0 35.0 94-95 29.033485199948203 34.0 29.0 35.0 2.0 35.0 96-97 28.639795005904908 34.0 29.0 35.0 2.0 35.0 98-99 28.285585018554357 34.0 29.0 35.0 2.0 35.0 100-101 27.262245376104 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.04253234802314765 1101 2 0.10006245758875565 1101 3 0.108106423482905 1101 4 0.10296183352625121 1101 5 0.14784660631078594 1101 6 0.10070239107238876 1101 7 0.15210467539441908 1101 8 0.1206250616767619 1101 9 0.11337624832319193 1101 10-11 0.18988120485585824 1101 12-13 0.1625498550287361 1101 14-15 0.13295330608810474 1101 16-17 0.14851560114603757 1101 18-19 0.02907032419558675 1101 20-21 0.012875474229524286 1101 22-23 0.06190143103583523 1101 24-25 0.09298096274076073 1101 26-27 0.11290789574954374 1101 28-29 -0.010066680701662278 1101 30-31 0.0803101202819434 1101 32-33 0.04539365056070466 1101 34-35 0.10069914373625721 1101 36-37 0.16056613706351186 1101 38-39 0.08140549862633861 1101 40-41 -0.0494736116572696 1101 42-43 -0.1318448626139599 1101 44-45 -0.030299113216663898 1101 46-47 -0.04524158751473095 1101 48-49 0.019430604640113813 1101 50-51 -0.09940341944670195 1101 52-53 -0.06092616268883688 1101 54-55 0.010467319995804303 1101 56-57 -0.032093989560323166 1101 58-59 -0.21146695103745827 1101 60-61 -0.09204181090208863 1101 62-63 -0.13804035830587935 1101 64-65 -0.15320576897176608 1101 66-67 -0.08700689256895089 1101 68-69 -0.3305890148995516 1101 70-71 -0.17681931446022503 1101 72-73 -0.18543492302660525 1101 74-75 -0.29581308314591226 1101 76-77 -0.1941596132649721 1101 78-79 -0.09613652830359243 1101 80-81 -0.2778408557261436 1101 82-83 -0.22448816032477126 1101 84-85 -0.20595922488504925 1101 86-87 -0.3226637689412044 1101 88-89 -0.19059666438576883 1101 90-91 -0.29984956451472655 1101 92-93 -0.163353729786337 1101 94-95 -0.20166518619742035 1101 96-97 -0.020041916489887512 1101 98-99 -0.3298992906895748 1101 100-101 -0.11626311970024261 1102 1 -0.09849838487464879 1102 2 -0.021595919181532253 1102 3 -0.04270374265338717 1102 4 -0.018422157036376063 1102 5 -0.06600446858342934 1102 6 0.014158714471314227 1102 7 -0.07001987048449365 1102 8 -0.09911016425618158 1102 9 -0.10944062791507747 1102 10-11 -0.04151063047496706 1102 12-13 0.04297487524018351 1102 14-15 0.020579629687411227 1102 16-17 0.01342560476947341 1102 18-19 -0.09787128057197947 1102 20-21 -0.06404731524914808 1102 22-23 -0.12533315512800414 1102 24-25 -0.14399386402882186 1102 26-27 -0.23690302332681767 1102 28-29 -0.13468347625840238 1102 30-31 -0.12058690333810063 1102 32-33 -0.08656874547066451 1102 34-35 -0.15632850217298255 1102 36-37 -0.21974747791276883 1102 38-39 -0.24415903395458116 1102 40-41 -0.03466008258579478 1102 42-43 -0.05699660040422572 1102 44-45 -0.04780008313618822 1102 46-47 -0.025607246352059576 1102 48-49 -0.043088715729645344 1102 50-51 0.0022368778923507193 1102 52-53 0.09140097071525588 1102 54-55 -0.10759557907967832 1102 56-57 0.06269663101405598 1102 58-59 -0.10757386984049333 1102 60-61 -0.18546189659664947 1102 62-63 -0.30543750330958375 1102 64-65 -0.24324986129956017 1102 66-67 -0.21346415352936532 1102 68-69 -0.2235591762002045 1102 70-71 -0.1086389820299658 1102 72-73 -0.20743439774251016 1102 74-75 -0.21076075019791674 1102 76-77 -0.2250050250118214 1102 78-79 -0.23404076957028863 1102 80-81 -0.2608940786019822 1102 82-83 -0.3198413506454827 1102 84-85 -0.38056999232216526 1102 86-87 -0.39731500854926693 1102 88-89 -0.33348211171659514 1102 90-91 -0.3228016299587537 1102 92-93 -0.2738119177959959 1102 94-95 -0.23075188993948714 1102 96-97 -0.2928037758707731 1102 98-99 -0.3394423625076364 1102 100-101 -0.4110015814358121 1103 1 -0.02902130448947915 1103 2 0.004995664575062619 1103 3 -0.050581176729906474 1103 4 -0.07136312432572112 1103 5 0.018323159299505676 1103 6 0.00670447925865858 1103 7 0.011034649618160586 1103 8 0.013536058559452613 1103 9 -0.08870884360391784 1103 10-11 -0.0847388307524426 1103 12-13 -0.06705073442805087 1103 14-15 0.0036110282016110773 1103 16-17 -0.03970897137634921 1103 18-19 -0.08227723471265591 1103 20-21 0.04864926436415118 1103 22-23 0.021647240272898216 1103 24-25 -0.10816975233660742 1103 26-27 -0.05043873718318537 1103 28-29 -0.08428758691994886 1103 30-31 -0.09649647194512312 1103 32-33 -0.04186145087238202 1103 34-35 -0.026049329635206675 1103 36-37 -0.028126622060739237 1103 38-39 -0.0539448897809649 1103 40-41 0.07017116875876894 1103 42-43 0.11791786239593449 1103 44-45 0.2428701137185385 1103 46-47 0.357771958745289 1103 48-49 0.27871207025673783 1103 50-51 0.22054603786109794 1103 52-53 0.26459632474161765 1103 54-55 0.14841791788718695 1103 56-57 0.17808099419434598 1103 58-59 0.15268531886693637 1103 60-61 0.2047692797759737 1103 62-63 0.06273508381167403 1103 64-65 0.08047520168216238 1103 66-67 0.003738417912337866 1103 68-69 0.006310693932213951 1103 70-71 0.052661743301285924 1103 72-73 0.16926681503245078 1103 74-75 0.16406868611616332 1103 76-77 0.06313564649229164 1103 78-79 0.08855408174558832 1103 80-81 0.07254127138238076 1103 82-83 -0.03107689376792422 1103 84-85 -0.04303989488806792 1103 86-87 -0.08020736681694984 1103 88-89 -0.07640717079756243 1103 90-91 -0.09140546126196725 1103 92-93 -0.19306950055033667 1103 94-95 -0.41909005424674106 1103 96-97 -0.23535331111283853 1103 98-99 -0.3077306946022311 1103 100-101 -0.35921861032253943 1104 1 -0.023290409046307303 1104 2 -0.08355794181116849 1104 3 0.01552512665176664 1104 4 -0.140965849201109 1104 5 -0.032862235248806826 1104 6 -0.06900820967445753 1104 7 0.029801647586779723 1104 8 -0.0824622820700256 1104 9 -0.056045637188184116 1104 10-11 -0.09777811979952844 1104 12-13 -0.10611336852749531 1104 14-15 -0.05796051498747801 1104 16-17 -0.06094705314810511 1104 18-19 -0.06723969918719064 1104 20-21 -0.13097950671426872 1104 22-23 -0.1571674382563515 1104 24-25 -0.10083943546379714 1104 26-27 -0.00951126564476823 1104 28-29 -0.0463479255341781 1104 30-31 -0.12604606433377086 1104 32-33 -0.0441762251672273 1104 34-35 -0.02081684866384137 1104 36-37 -0.04681217253617831 1104 38-39 -0.05053072303257977 1104 40-41 0.03769456505040836 1104 42-43 0.034461670950385326 1104 44-45 0.2016485474760259 1104 46-47 0.14922597111986846 1104 48-49 0.21337087196485527 1104 50-51 0.26200803778326076 1104 52-53 0.2606972968601511 1104 54-55 0.12692257760151904 1104 56-57 0.20393876758218 1104 58-59 0.1035376411784128 1104 60-61 0.09665293723662671 1104 62-63 0.1139774108799898 1104 64-65 0.19825430996254312 1104 66-67 0.07188468996243103 1104 68-69 0.07549751400285487 1104 70-71 0.14228606344627082 1104 72-73 0.20369718137707338 1104 74-75 0.16951402673323557 1104 76-77 0.10938469414719165 1104 78-79 0.304902713725113 1104 80-81 0.2012344287222838 1104 82-83 0.14230221614395688 1104 84-85 0.20212232795465113 1104 86-87 0.1712998579198377 1104 88-89 0.17401871844166905 1104 90-91 -0.010633850569600867 1104 92-93 -0.11801323507552297 1104 94-95 -0.07234657739644135 1104 96-97 0.013734196549680888 1104 98-99 -0.006005290852943546 1104 100-101 0.07503728758912587 1105 1 0.03979337919624015 1105 2 0.08333308412358065 1105 3 0.051050648915882846 1105 4 0.10922576934238037 1105 5 0.0836100571290217 1105 6 0.09986266331252835 1105 7 0.03393468067385186 1105 8 0.1899506531127031 1105 9 0.19505420743067958 1105 10-11 0.1266937666258059 1105 12-13 0.0059359038094939365 1105 14-15 0.10355071431737883 1105 16-17 0.162232395204974 1105 18-19 0.11165290649157811 1105 20-21 0.06930486475838649 1105 22-23 0.1397220726683912 1105 24-25 0.078457270374102 1105 26-27 0.18530795583507143 1105 28-29 0.20538141841579005 1105 30-31 0.18628855438578995 1105 32-33 0.18270841705740537 1105 34-35 0.27257473423694023 1105 36-37 0.2404237316800888 1105 38-39 0.3467357075628996 1105 40-41 0.3283542066462175 1105 42-43 0.493639658108485 1105 44-45 0.5643172288265248 1105 46-47 0.3236548542689235 1105 48-49 0.44734457162513763 1105 50-51 0.44736590478956373 1105 52-53 0.5197388551249631 1105 54-55 0.4529805157177478 1105 56-57 0.41677910132114704 1105 58-59 0.29148560009727476 1105 60-61 0.4113056480028803 1105 62-63 0.4173619666301178 1105 64-65 0.3253687189555876 1105 66-67 0.17652089355403433 1105 68-69 0.3354982373860125 1105 70-71 0.3868593249040444 1105 72-73 0.4827248315770518 1105 74-75 0.41878235684319165 1105 76-77 0.37142580648820456 1105 78-79 0.402503626162801 1105 80-81 0.44349574006580994 1105 82-83 0.3964471431319794 1105 84-85 0.40579495008600475 1105 86-87 0.39779941072866265 1105 88-89 0.4187622966613205 1105 90-91 0.4429972891449374 1105 92-93 0.5488351872960067 1105 94-95 0.5927219032006086 1105 96-97 0.5368614969714862 1105 98-99 0.5753332468094854 1105 100-101 0.6792437021453885 1106 1 -0.09222233397811408 1106 2 -0.021102246389741453 1106 3 0.031109101469052547 1106 4 0.02761914302642765 1106 5 -0.02197545042135829 1106 6 -0.08495062067391501 1106 7 -0.021550906204360842 1106 8 -0.027064084611680528 1106 9 -0.07717242189373508 1106 10-11 -0.1518142788111163 1106 12-13 -0.17930615119976068 1106 14-15 -0.09215022257441063 1106 16-17 -0.10367466488515475 1106 18-19 -0.09317385206079365 1106 20-21 -0.01930463842337815 1106 22-23 -0.10204216271567645 1106 24-25 -0.14022065881676582 1106 26-27 -0.05121337280004923 1106 28-29 -0.0904115786197579 1106 30-31 -0.0545387125829393 1106 32-33 -0.0041544066502083865 1106 34-35 0.028289765340765882 1106 36-37 -0.007626736853381999 1106 38-39 -6.302928710724132E-4 1106 40-41 -0.17277013355657544 1106 42-43 -0.06713870279836698 1106 44-45 0.01799123760623189 1106 46-47 0.012598364220373526 1106 48-49 0.09135049176348531 1106 50-51 0.31662156092144755 1106 52-53 0.19504281562700498 1106 54-55 0.1266592736579213 1106 56-57 0.15635465030460693 1106 58-59 0.1066600545623757 1106 60-61 0.32822489891862006 1106 62-63 0.06521056847420681 1106 64-65 0.054836318142207574 1106 66-67 -0.12420589878328059 1106 68-69 -0.007764906610766786 1106 70-71 0.02542177439357829 1106 72-73 0.08871458705331037 1106 74-75 0.14647821701599284 1106 76-77 0.06952190549845838 1106 78-79 0.29448682871723264 1106 80-81 0.2303699385491882 1106 82-83 0.3252182754181021 1106 84-85 0.23069970960310116 1106 86-87 0.26101127298855786 1106 88-89 0.22212296813874843 1106 90-91 0.17004350620543462 1106 92-93 0.1997994814479256 1106 94-95 0.18078335895733133 1106 96-97 0.3612673099964496 1106 98-99 0.13636729384522184 1106 100-101 0.11083072093161306 1107 1 0.0037867483265934254 1107 2 -0.050175697054754664 1107 3 -0.08945835978781957 1107 4 -0.03529494288275714 1107 5 -0.04147195583833252 1107 6 0.048466349040886314 1107 7 -0.04543698417968045 1107 8 0.028753837737554022 1107 9 -0.11313027022745104 1107 10-11 0.03798591672682505 1107 12-13 0.035201439604342966 1107 14-15 0.03140258047039168 1107 16-17 -0.02294660929282344 1107 18-19 -0.07657931954403097 1107 20-21 -0.05536848115844606 1107 22-23 -0.03534682963467617 1107 24-25 -0.03045760478704551 1107 26-27 0.031304661851990545 1107 28-29 -0.08564628878804825 1107 30-31 -0.13260821459426353 1107 32-33 0.013661234868926897 1107 34-35 0.09741004078800586 1107 36-37 -0.08400222319046691 1107 38-39 0.04555094395764314 1107 40-41 -0.01902174353462982 1107 42-43 0.07653899308173351 1107 44-45 -0.036025337198616114 1107 46-47 -0.18916872183192623 1107 48-49 -0.31118637444907193 1107 50-51 -0.17331365209052052 1107 52-53 -0.12191131766424235 1107 54-55 -0.22141876140427286 1107 56-57 -0.22428183325812512 1107 58-59 -0.09971750930956347 1107 60-61 -0.20063243908159478 1107 62-63 -0.31202450905217205 1107 64-65 -0.31094824327726656 1107 66-67 -0.12799539367927792 1107 68-69 -0.17280398521960905 1107 70-71 -0.14302762671018598 1107 72-73 -0.0117051329431348 1107 74-75 -0.03876288179486309 1107 76-77 -0.09459053015257624 1107 78-79 -0.1522684274236461 1107 80-81 -0.20305153397719877 1107 82-83 -0.189039922039548 1107 84-85 -0.24728700309032803 1107 86-87 -0.07695360329780954 1107 88-89 -0.22484004148701686 1107 90-91 -0.19997336373419827 1107 92-93 -0.19688707337959954 1107 94-95 -0.04965437674367834 1107 96-97 0.03757077363936645 1107 98-99 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-1.353204585717414 2316 60-61 -0.9884712670423283 2316 62-63 -1.0571766020467877 2316 64-65 -1.037413944078672 2316 66-67 -1.070500508926962 2316 68-69 -1.0396515505613806 2316 70-71 -1.044777987093184 2316 72-73 -0.9236442513733891 2316 74-75 -0.9488931521361899 2316 76-77 -1.1591899101662548 2316 78-79 -1.0483763465314588 2316 80-81 -1.0606913761685455 2316 82-83 -1.101689684122988 2316 84-85 -1.1325769151951448 2316 86-87 -0.9707315818584199 2316 88-89 -1.0948987455059438 2316 90-91 -1.2578735580276366 2316 92-93 -1.0454858643563512 2316 94-95 -1.2111317501270875 2316 96-97 -1.0734992934675809 2316 98-99 -1.2310516491718282 2316 100-101 -1.5827438964068392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 6.0 4 17.0 5 46.0 6 101.0 7 333.0 8 701.0 9 1467.0 10 2494.0 11 4079.0 12 5992.0 13 8564.0 14 11323.0 15 15102.0 16 19251.0 17 24706.0 18 22265.0 19 16306.0 20 14871.0 21 15348.0 22 16365.0 23 18636.0 24 22043.0 25 27336.0 26 34482.0 27 43461.0 28 55481.0 29 69492.0 30 87481.0 31 109049.0 32 135347.0 33 171004.0 34 224265.0 35 299181.0 36 425773.0 37 568115.0 38 522389.0 39 103658.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47528352115192 20.656469523111916 14.815832013697289 20.052414942038872 2 14.730240338967723 23.605633529901887 38.61580643702809 23.048319694102297 3 22.962914381843362 26.15423086329571 29.798435857863183 21.084418896997747 4 12.686685976160128 17.21843183618557 40.483701842389195 29.611180345265108 5 12.280269471742509 40.19990460434215 34.55802972120506 12.961796202710282 6 28.332465697153207 39.788137454239184 16.97913410339812 14.900262745209487 7 26.830916847861364 34.39820808589614 21.619581880250355 17.151293185992145 8 24.432462037921226 33.43992414850333 19.503697146881862 22.623916666693578 9 23.71786653456381 21.028606748677653 22.87981212803686 32.373714588721676 10-11 24.672173089831297 26.628534096888956 27.752456713736557 20.946836099543187 12-13 28.25613572664268 24.335823071733724 26.95658787105891 20.451453330564682 14-15 22.80654049069343 26.97893473444415 28.33445099468092 21.8800737801815 16-17 20.188243340953576 30.086221011135844 28.71134804656884 21.01418760134174 18-19 20.0123878721569 31.616265314894477 29.141823374648844 19.229523438299776 20-21 22.15312829841082 27.96211018193986 28.037105627952823 21.847655891696498 22-23 20.92048736395058 28.26923083133408 28.53615459909442 22.274127205620918 24-25 23.0177709128499 28.34691338209901 28.53365183555907 20.10166386949202 26-27 22.60175126258039 26.631399193401883 30.94824126891852 19.81860827509921 28-29 20.605891877759692 30.27658820981734 29.54290930741765 19.574610605005322 30-31 21.548985971203447 28.58571779919409 28.42269741171425 21.44259881788822 32-33 21.637444370523 28.79530290209858 28.860443485618692 20.706809241759732 34-35 23.26402811072149 27.415252424041057 29.17491770492624 20.14580176031121 36-37 22.90308357949104 26.973560320616034 30.44122658406319 19.68212951582973 38-39 21.75382144401064 28.32692960778776 30.57705875618027 19.342190192021334 40-41 21.242123282140724 29.0977665810765 29.21095554155968 20.4491545952231 42-43 20.392743342083854 29.902550461364275 27.81850733891003 21.88619885764184 44-45 22.10463360026248 27.63514357950731 27.963570742791156 22.296652077439056 46-47 23.292065171337633 29.05722320395172 27.5949306588955 20.055780965815146 48-49 22.93726266131926 28.49157311444217 28.3212560191463 20.249908205092268 50-51 20.741079585619865 29.113453338961055 29.928982873669863 20.21648420174922 52-53 21.808806950384245 30.585659584760847 27.2354118757583 20.370121589096605 54-55 20.557893441693846 28.768120411667464 28.28947899567813 22.384507150960562 56-57 22.694318003833317 28.209934652765106 27.742492818585934 21.35325452481564 58-59 24.379769690446356 27.706707240572353 27.161329877252953 20.752193191728335 60-61 21.944580092318066 28.578713877294227 29.73136553484665 19.74534049554106 62-63 21.598565547660126 28.540924703687175 28.910971548187565 20.949538200465135 64-65 20.97571676603488 30.63605776903886 27.980109342722425 20.408116122203836 66-67 21.85661580930776 27.92412855352653 27.55558268895546 22.66367294821025 68-69 21.251803639961814 29.20747058952707 27.760602561071863 21.780123209439253 70-71 22.979322420493794 28.789515742678223 27.10140408739809 21.129757749429892 72-73 22.331965263579754 27.208155973515275 29.12574110057654 21.334137662328438 74-75 20.988678949155624 30.84215746002077 27.69305069647076 20.476112894352845 76-77 22.044502687968038 28.402587760614384 27.062882660434408 22.490026890983174 78-79 21.831880815494014 28.812653455735155 27.908719854213217 21.446745874557614 80-81 22.893305847055885 28.834885504971542 27.432255406012224 20.839553241960353 82-83 22.23920154415666 28.07023690998157 28.811442441121276 20.879119104740493 84-85 21.119530629732953 28.783063349517345 28.36894445781944 21.728461562930264 86-87 21.612353237806545 28.957644293315603 27.18641416402811 22.243588304849737 88-89 21.611217805983834 28.53647753632479 27.103249814230356 22.74905484346102 90-91 22.10595764290699 26.246190551696273 28.1289468984624 23.518904906934335 92-93 22.259255946162487 28.35652076470244 28.133581047718177 21.25064224141689 94-95 21.569567136061842 28.279596786577166 28.443600644389676 21.707235432971313 96-97 19.25059578084307 28.734438611281238 29.902296624341833 22.112668983533858 98-99 20.938708193985335 29.782365228039854 26.958880875706104 22.320045702268708 100-101 20.250664008211757 28.78347430539711 27.148114400755297 23.817747285635836 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 171.0 1 118.0 2 106.0 3 162.0 4 166.0 5 182.5 6 263.0 7 510.5 8 855.5 9 1097.5 10 1242.0 11 1343.0 12 1508.0 13 1759.5 14 2059.0 15 2349.5 16 2662.0 17 3012.5 18 3307.5 19 3695.5 20 4185.5 21 4608.0 22 5311.0 23 6496.5 24 8021.0 25 9975.5 26 12664.0 27 16789.5 28 22699.5 29 30134.0 30 38592.5 31 47074.5 32 55923.0 33 64229.0 34 72164.5 35 79751.0 36 85652.5 37 91626.0 38 98392.0 39 102298.0 40 103474.0 41 105044.5 42 107933.0 43 112392.0 44 114777.5 45 117945.5 46 127257.5 47 175347.5 48 291844.0 49 280646.5 50 160982.0 51 110697.0 52 83756.0 53 66937.0 54 55953.0 55 46689.5 56 37705.5 57 29310.0 58 22758.0 59 18176.0 60 14137.5 61 10301.5 62 7230.0 63 4835.0 64 3095.0 65 1885.0 66 1038.0 67 590.0 68 316.0 69 168.5 70 108.0 71 65.0 72 30.0 73 23.0 74 19.5 75 13.5 76 10.0 77 5.5 78 1.0 79 2.0 80 2.5 81 1.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05354299279494745 2 0.0037783655953008755 3 0.043079826531037334 4 0.0 5 0.0 6 0.0013240426445071446 7 0.0 8 0.0 9 0.0 10-11 3.713778149227357E-4 12-13 0.0 14-15 0.0026319384274959092 16-17 0.0 18-19 0.0 20-21 0.012562258261299494 22-23 0.0 24-25 0.0 26-27 0.007734346667303929 28-29 0.010705369186685815 30-31 0.010931425247943131 32-33 0.006361863438241646 34-35 0.008057283897671526 36-37 0.021378444650334873 38-39 0.010737662909722574 40-41 0.013789419736696359 42-43 0.0 44-45 0.0 46-47 3.229372303675962E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.001356336367543904 58-59 1.7761547670217792E-4 60-61 0.010495459986946877 62-63 0.015581721365236518 64-65 0.04375799471480929 66-67 0.024591670092492454 68-69 0.0019699171052423373 70-71 3.39084091885976E-4 72-73 0.0 74-75 1.614686151837981E-5 76-77 0.0 78-79 0.0 80-81 4.844058455513943E-5 82-83 0.0 84-85 8.073430759189906E-5 86-87 9.203711065476492E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 1.1302803062865867E-4 96-97 3.39084091885976E-4 98-99 4.198183994778751E-4 100-101 0.0013401895060255243 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3096577.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.43889674002612 #Duplication Level Percentage of deduplicated Percentage of total 1 77.86240479755948 37.715689859190974 2 13.393848718729965 12.975665100761837 3 3.7482849149286457 5.446883578392788 4 1.6008856636431423 3.101805414151934 5 0.85410438450857 2.0685937043207088 6 0.5036868098127217 1.463882002189896 7 0.3601517295840333 1.2211746708043958 8 0.2414455670690943 0.9356285513277527 9 0.1718771313156719 0.7492984754194566 >10 1.0916465573107133 10.088805276074297 >50 0.10638552519737117 3.5758726540458743 >100 0.05718578608279344 5.0621050226649436 >500 0.003812129030525954 1.2821882609032447 >1k 0.0026751782670045455 3.0163609230063444 >5k 6.687945667511364E-4 2.130371717944584 >10k+ 9.363123934515909E-4 9.16567478880101 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 73388 2.369971746221715 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 24034 0.7761473394654808 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 20906 0.6751325738064967 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19604 0.6330861464126356 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 18476 0.5966588268271708 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 17528 0.5660443773883227 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 16988 0.5486057669484724 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 16605 0.5362372710253935 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 14455 0.4668057664963603 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 13719 0.44303758634130525 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 13434 0.4338338752758288 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 12724 0.4109053319197294 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 10844 0.35019313261062135 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 10224 0.33017102432783035 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 7931 0.2561215174045406 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7924 0.2558954613432832 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7785 0.2514066338411737 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 6800 0.21959731664996543 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 6307 0.20367651119284294 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6258 0.2020941187640417 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 6081 0.19637812978653527 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5711 0.1844294522629342 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 5649 0.1824272414346551 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 5315 0.1716411379403774 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4906 0.1584330052183427 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4388 0.14170485668530122 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4214 0.13608574887690505 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 4040 0.13046664106850886 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3748 0.12103687394177506 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 3647 0.11777520791506234 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3612 0.11664492760877576 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 3599 0.11622510920929789 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 3508 0.11328638041295275 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3502 0.1130926180747322 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 3411 0.11015388927838707 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3332 0.10760268515848305 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 3.229372303675962E-5 0.0 0.0 0.0 3 0.0 3.229372303675962E-5 0.0 0.0 0.0 4 0.0 3.229372303675962E-5 0.0 0.0 0.0 5 0.0 3.229372303675962E-5 0.0 0.0 0.0 6 0.0 3.229372303675962E-5 0.0 0.0 0.0 7 0.0 3.229372303675962E-5 0.0 0.0 0.0 8 0.0 3.229372303675962E-5 0.0 0.0 0.0 9 0.0 3.229372303675962E-5 0.0 3.229372303675962E-5 0.0 10-11 0.0 3.229372303675962E-5 0.0 4.844058455513943E-5 0.0 12-13 0.0 3.229372303675962E-5 0.0 6.458744607351924E-5 0.0 14-15 0.0 3.229372303675962E-5 0.0 8.073430759189906E-5 0.0 16-17 0.0 3.229372303675962E-5 0.0 2.0990919973893756E-4 0.0 18-19 0.0 3.229372303675962E-5 0.0 3.3908409188597606E-4 0.0 20-21 0.0 3.229372303675962E-5 0.0 6.135807376984328E-4 0.0 22-23 0.0 3.229372303675962E-5 0.0 0.0010333991371763078 0.0 24-25 0.0 3.229372303675962E-5 0.0 0.0013886300905806637 0.0 26-27 0.0 3.229372303675962E-5 0.0 0.0019053296591688175 0.0 28-29 0.0 3.229372303675962E-5 0.0 0.00427891830237065 0.0 30-31 0.0 3.229372303675962E-5 0.0 0.009300592234586772 0.0 32-33 0.0 3.229372303675962E-5 0.0 0.018245953515769187 0.0 34-35 0.0 3.229372303675962E-5 0.0 0.028709119779679305 0.0 36-37 0.0 3.229372303675962E-5 0.0 0.039592104443067294 0.0 38-39 0.0 3.229372303675962E-5 0.0 0.047213423079742564 0.0 40-41 0.0 3.229372303675962E-5 0.0 0.05938815666460094 0.0 42-43 0.0 3.229372303675962E-5 0.0 0.07771484448796204 0.0 44-45 0.0 3.229372303675962E-5 0.0 0.10574579608386939 0.0 46-47 0.0 3.229372303675962E-5 0.0 0.17377252366080354 0.0 48-49 0.0 3.229372303675962E-5 0.0 0.3458173331391404 0.0 50-51 0.0 3.229372303675962E-5 0.0 0.44145519391250404 0.0 52-53 0.0 6.458744607351924E-5 0.0 0.531199450231659 0.0 54-55 0.0 6.458744607351924E-5 0.0 0.6598092022255542 0.0 56-57 0.0 6.458744607351924E-5 0.0 0.7362968852381193 0.0 58-59 0.0 6.458744607351924E-5 0.0 0.8549601705366926 0.0 60-61 0.0 6.458744607351924E-5 0.0 1.0890412219686447 0.0 62-63 0.0 6.458744607351924E-5 0.0 1.2003738321378736 0.0 64-65 0.0 6.458744607351924E-5 0.0 1.3210554751262442 0.0 66-67 0.0 6.458744607351924E-5 0.0 1.7047533453875037 0.0 68-69 1.614686151837981E-5 6.458744607351924E-5 0.0 2.073822159113111 0.0 70-71 3.229372303675962E-5 9.688116911027886E-5 0.0 2.290109369151809 0.0 72-73 3.229372303675962E-5 9.688116911027886E-5 0.0 2.5052662988842194 0.0 74-75 3.229372303675962E-5 9.688116911027886E-5 0.0 2.659113595431342 0.0 76-77 3.229372303675962E-5 9.688116911027886E-5 0.0 2.7486317956892403 0.0 78-79 3.229372303675962E-5 9.688116911027886E-5 0.0 2.8947931861536143 0.0 80-81 3.229372303675962E-5 9.688116911027886E-5 0.0 3.176055366942272 0.0 82-83 3.229372303675962E-5 9.688116911027886E-5 0.0 3.551744393890415 0.0 84-85 3.229372303675962E-5 9.688116911027886E-5 0.0 4.077647673544046 0.0 86-87 3.229372303675962E-5 9.688116911027886E-5 0.0 5.25289698915932 0.0 88-89 3.229372303675962E-5 9.688116911027886E-5 0.0 6.527610971727814 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 350 0.0 29.866774 2 TACCTGG 2620 0.0 29.742414 2 ACCTGGG 2810 0.0 26.545992 3 TAACGCA 400 0.0 26.120768 4 GTACCTG 3605 0.0 22.685305 1 ATAACGC 495 0.0 22.0764 3 GAGTACG 950 0.0 21.52119 1 GTATAAC 915 0.0 20.785494 1 GTACGCA 860 0.0 20.441319 3 GTACCCG 350 3.6379788E-12 20.377207 1 ACCGATC 165 6.33582E-5 20.147926 8 TACGCAG 875 0.0 20.08246 4 CTAACGC 170 7.943648E-5 19.56387 3 CCGCGTA 85 7.9699734E-5 19.555971 10-11 GAGTACT 9640 0.0 19.484655 12-13 TCACGCA 370 7.2759576E-12 19.253637 4 AGTACGC 890 0.0 19.21968 2 GTACTTT 9840 0.0 18.970566 14-15 AGTACTT 9765 0.0 18.846153 12-13 GGACCGT 285 4.2862666E-8 18.33066 6 >>END_MODULE