##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003844_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 823678 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.901494273247554 31.0 31.0 34.0 28.0 34.0 2 30.79134079094015 31.0 31.0 34.0 27.0 34.0 3 30.92464895262469 31.0 31.0 34.0 27.0 34.0 4 34.412630906737824 37.0 35.0 37.0 32.0 37.0 5 34.40858320848681 37.0 35.0 37.0 30.0 37.0 6 34.52092565298575 37.0 35.0 37.0 31.0 37.0 7 34.52407494190691 37.0 35.0 37.0 31.0 37.0 8 34.50446412311607 37.0 35.0 37.0 30.0 37.0 9 35.96499117373537 39.0 35.0 39.0 31.0 39.0 10-11 35.79727696988386 39.0 35.0 39.0 30.0 39.0 12-13 35.68341329985746 39.0 35.0 39.0 30.0 39.0 14-15 36.62611056747904 39.0 36.0 41.0 30.0 41.0 16-17 36.710020177787925 39.0 36.0 41.0 30.5 41.0 18-19 36.68001937650392 39.0 36.0 41.0 30.5 41.0 20-21 36.59323000007284 39.0 36.0 41.0 30.0 41.0 22-23 36.499459740335425 39.0 36.0 41.0 30.0 41.0 24-25 36.198105327567326 39.0 36.0 41.0 29.0 41.0 26-27 36.0630088456897 39.0 35.5 40.5 28.0 41.0 28-29 35.92736360568086 39.0 35.0 40.0 27.5 41.0 30-31 35.6479473774946 38.0 35.0 40.0 27.0 41.0 32-33 35.37737623197414 38.0 34.5 40.0 27.0 41.0 34-35 35.29234421703627 38.0 35.0 40.0 26.0 41.0 36-37 35.11552876245329 38.0 34.5 40.0 25.0 41.0 38-39 35.08496766940479 38.0 34.5 40.0 25.5 41.0 40-41 35.309060093871636 38.0 35.0 40.0 26.0 41.0 42-43 35.144685180373884 38.0 35.0 40.0 25.0 41.0 44-45 34.88810797423265 38.0 34.0 40.0 24.0 41.0 46-47 34.584352744640505 38.0 34.0 40.0 23.5 41.0 48-49 34.25529090251287 38.0 33.0 40.0 23.0 41.0 50-51 33.383859954982405 37.5 32.5 39.5 20.0 40.5 52-53 33.54305019680021 38.0 33.0 39.5 20.5 40.5 54-55 33.89180602128502 38.0 33.0 40.0 20.5 41.0 56-57 33.53953608084713 38.0 33.0 40.0 17.0 41.0 58-59 33.19157850519257 38.0 32.0 40.0 14.0 41.0 60-61 32.773016882811 37.0 31.0 40.0 13.0 41.0 62-63 32.58785836212695 37.0 31.0 40.0 12.0 41.0 64-65 32.31018431960062 36.5 31.0 39.5 8.0 41.0 66-67 31.784673136832595 36.0 30.5 39.0 7.5 40.5 68-69 31.3338707601757 35.5 30.0 39.0 6.5 40.0 70-71 30.695944895942347 35.0 29.0 37.5 2.0 40.0 72-73 30.269864558723192 35.0 29.0 37.0 2.0 39.5 74-75 29.92271069519885 34.5 29.0 37.0 2.0 39.0 76-77 29.502981747721805 34.0 29.0 36.0 2.0 39.0 78-79 28.90915989015125 34.0 28.0 36.0 2.0 38.0 80-81 28.32599268160616 34.0 26.0 35.5 2.0 37.0 82-83 27.85548964522544 33.5 26.0 35.0 2.0 37.0 84-85 27.56108940629712 33.0 26.0 35.0 2.0 36.0 86-87 27.48716367318297 33.5 26.0 35.0 2.0 36.0 88-89 27.14716309043097 33.5 25.5 35.0 2.0 36.0 90-91 26.691418855426512 33.0 25.0 35.0 2.0 35.0 92-93 26.387361323235538 33.0 24.0 35.0 2.0 35.0 94-95 26.11114112553692 32.5 23.5 35.0 2.0 35.0 96-97 25.794891328893087 32.0 21.5 35.0 2.0 35.0 98-99 25.5739305893808 32.0 20.0 35.0 2.0 35.0 100-101 25.26815515286313 32.0 19.0 35.0 2.0 35.0 102-103 24.734700331925826 32.0 17.5 34.5 2.0 35.0 104-105 24.208165690961756 31.0 11.5 34.0 2.0 35.0 106-107 23.69641534677386 31.0 7.0 34.0 2.0 35.0 108-109 23.415860324058674 31.0 3.5 34.0 2.0 35.0 110-111 23.095406821597763 31.0 2.0 34.0 2.0 35.0 112-113 22.397665106995696 30.5 2.0 34.0 2.0 35.0 114-115 21.7669398721345 30.0 2.0 34.0 2.0 35.0 116-117 20.969341174585217 29.0 2.0 34.0 2.0 35.0 118-119 20.138199636265625 29.0 2.0 34.0 2.0 35.0 120-121 18.50803712130226 26.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7238633354967483 1101 2 0.5930831634791502 1101 3 0.609638203813315 1101 4 0.7495689977515454 1101 5 0.8606450492505004 1101 6 0.7979446326848532 1101 7 0.7249657060298276 1101 8 0.6917034314538171 1101 9 0.8345124131241306 1101 10-11 1.007033609011465 1101 12-13 0.915456225708887 1101 14-15 1.2363483602471703 1101 16-17 1.0020940487126992 1101 18-19 0.7907482254821332 1101 20-21 1.024531518256076 1101 22-23 0.8870850215316608 1101 24-25 0.8615439579605919 1101 26-27 0.9028935980236099 1101 28-29 0.9892887592296233 1101 30-31 0.9392600502952106 1101 32-33 1.0440668841828682 1101 34-35 1.134950921591333 1101 36-37 1.2636451395139474 1101 38-39 1.164625770225065 1101 40-41 1.0966769307494886 1101 42-43 1.007691818152253 1101 44-45 1.3041152235783287 1101 46-47 1.2518961876488817 1101 48-49 1.4370872993553547 1101 50-51 1.0092574884702472 1101 52-53 1.0727325354399468 1101 54-55 1.3894277048083197 1101 56-57 1.4474013209562457 1101 58-59 1.5545481284527156 1101 60-61 1.4086226030492668 1101 62-63 1.3872305058189838 1101 64-65 1.5886823788843039 1101 66-67 1.6222537410783424 1101 68-69 1.4577459319271888 1101 70-71 1.147404014516841 1101 72-73 1.2364237741394355 1101 74-75 1.2008146118708432 1101 76-77 1.1557373112735796 1101 78-79 1.3685101504553572 1101 80-81 1.4729319434979509 1101 82-83 1.5176273889723824 1101 84-85 1.5661377752855543 1101 86-87 1.4426909489515296 1101 88-89 1.5759440073951687 1101 90-91 1.6846141428956365 1101 92-93 1.6723535945627148 1101 94-95 1.6620041827626366 1101 96-97 1.565256520170255 1101 98-99 1.523747597593193 1101 100-101 1.6213472627365597 1101 102-103 1.6116410434822832 1101 104-105 1.6562289401888464 1101 106-107 1.7465963538843035 1101 108-109 1.6184495839986326 1101 110-111 1.6583454689280508 1101 112-113 1.5844253332188778 1101 114-115 1.3547651776271117 1101 116-117 1.5599869466922769 1101 118-119 1.6211727298432557 1101 120-121 1.7626486959380259 1102 1 0.5523429320446773 1102 2 0.6097297504279702 1102 3 0.6062624435387356 1102 4 0.8218418670020782 1102 5 0.8742600996592458 1102 6 0.8409680462733178 1102 7 0.6025345211807718 1102 8 0.6636305288736821 1102 9 0.6814489584837986 1102 10-11 0.7951213277825033 1102 12-13 0.788329300947268 1102 14-15 0.9853461956444818 1102 16-17 0.854250410067479 1102 18-19 0.9001189426058005 1102 20-21 1.032112863879476 1102 22-23 0.9345134516801963 1102 24-25 1.049993703571964 1102 26-27 0.9803188955540278 1102 28-29 1.038448325143456 1102 30-31 0.9929391463527608 1102 32-33 0.8949396916916754 1102 34-35 0.9359695688648983 1102 36-37 0.9253189684019745 1102 38-39 0.9511496842128366 1102 40-41 0.9182661334162177 1102 42-43 0.9594852420218842 1102 44-45 0.9127594738125921 1102 46-47 0.9628903112579792 1102 48-49 0.9487198760339268 1102 50-51 0.7852764317033802 1102 52-53 0.933246567110352 1102 54-55 0.7224559721335666 1102 56-57 0.9926560419665478 1102 58-59 0.9915945704268836 1102 60-61 1.0159384501811388 1102 62-63 1.192799189837082 1102 64-65 0.9999106748037967 1102 66-67 1.0061661079079265 1102 68-69 0.7366217955599446 1102 70-71 0.6675053601766621 1102 72-73 0.7750854267958331 1102 74-75 0.8065514009190657 1102 76-77 0.7469775457464003 1102 78-79 0.9867835588076055 1102 80-81 1.161077306084838 1102 82-83 1.1951500799641934 1102 84-85 1.258920368856586 1102 86-87 1.3612977443651566 1102 88-89 1.265470362821656 1102 90-91 1.0392356197247352 1102 92-93 1.3025009004656063 1102 94-95 1.042990647848967 1102 96-97 1.2335279706361888 1102 98-99 1.3832817754585172 1102 100-101 1.4855616995528358 1102 102-103 1.3934218640278857 1102 104-105 1.4417217766015256 1102 106-107 1.590223388378618 1102 108-109 1.7471482816855044 1102 110-111 1.940423159678538 1102 112-113 1.8006481516099022 1102 114-115 1.8486681830281455 1102 116-117 1.8734206421890462 1102 118-119 1.8912777180822786 1102 120-121 2.023552756776386 1103 1 0.7160949193260748 1103 2 0.7778028008539621 1103 3 0.8709479435183525 1103 4 1.1595370282266515 1103 5 1.1535189965651966 1103 6 1.0990904220427495 1103 7 0.9055796477553102 1103 8 0.8651306715502969 1103 9 0.9828634951924542 1103 10-11 1.1699225996425966 1103 12-13 1.095267460826605 1103 14-15 1.292742958923597 1103 16-17 1.2870395340330774 1103 18-19 1.1977933138095835 1103 20-21 1.3157539710499861 1103 22-23 1.3870429042096433 1103 24-25 1.3418489071918742 1103 26-27 1.4738984479571187 1103 28-29 1.2583639421178958 1103 30-31 1.338542668426868 1103 32-33 1.4842042956621668 1103 34-35 1.4951090250631793 1103 36-37 1.4491357716305444 1103 38-39 1.4503140069065736 1103 40-41 1.5571312428695592 1103 42-43 1.64005268729521 1103 44-45 1.803968556198143 1103 46-47 1.7253439413586733 1103 48-49 1.7559535803264552 1103 50-51 1.463224742004627 1103 52-53 1.66487185755917 1103 54-55 1.5736136909383376 1103 56-57 1.6739062967462104 1103 58-59 1.6115957933692826 1103 60-61 1.5554802544288293 1103 62-63 1.900617868243991 1103 64-65 1.7303130228532062 1103 66-67 1.7027439074268962 1103 68-69 1.6609114698496192 1103 70-71 1.7051414125185964 1103 72-73 1.6216367292294898 1103 74-75 1.8194432520462094 1103 76-77 1.8708106548736296 1103 78-79 1.821462128780297 1103 80-81 1.9265067626907673 1103 82-83 1.9109382663406116 1103 84-85 2.098674149786838 1103 86-87 1.9120498539407933 1103 88-89 1.982471300410829 1103 90-91 2.0456254937616656 1103 92-93 1.8999897920721445 1103 94-95 2.102007809807308 1103 96-97 2.009963908836834 1103 98-99 1.994396283631847 1103 100-101 1.942065714880382 1103 102-103 1.859159094471 1103 104-105 2.06749121119449 1103 106-107 1.9417377987165558 1103 108-109 1.9627428355153533 1103 110-111 2.105612104279249 1103 112-113 2.1942623540476305 1103 114-115 2.1271423585611444 1103 116-117 2.153871907934434 1103 118-119 2.0418321186366803 1103 120-121 2.185276697912091 1104 1 0.5110827369383273 1104 2 0.6174593735422107 1104 3 0.7393947305171764 1104 4 0.9026792555317726 1104 5 0.9772887411494224 1104 6 0.8821444720651286 1104 7 0.7391698885853657 1104 8 0.5482725673618773 1104 9 0.7185968417855335 1104 10-11 0.807587724864284 1104 12-13 0.8460920510177132 1104 14-15 0.9553529733435653 1104 16-17 0.8634612269706068 1104 18-19 0.8814117315909016 1104 20-21 0.8539088779831729 1104 22-23 0.7486940735531249 1104 24-25 0.8064039599822266 1104 26-27 0.8783868705450857 1104 28-29 0.9158938179735614 1104 30-31 0.9586013427841849 1104 32-33 0.942821470342686 1104 34-35 0.8291675005243704 1104 36-37 0.7156938741614951 1104 38-39 0.9423995985396729 1104 40-41 0.8730611651343239 1104 42-43 1.0060381347286267 1104 44-45 1.0100042249035042 1104 46-47 1.1720356357879211 1104 48-49 1.1491836534207778 1104 50-51 1.0597846677500797 1104 52-53 1.1300816100992463 1104 54-55 1.0780681354380377 1104 56-57 1.5334897751079666 1104 58-59 1.212166790999106 1104 60-61 1.0268474995517352 1104 62-63 1.319393662585405 1104 64-65 1.0075907978685343 1104 66-67 1.03337971204461 1104 68-69 1.1219568347411375 1104 70-71 1.2120820585995133 1104 72-73 1.1206795416207171 1104 74-75 1.2718366354350685 1104 76-77 1.2815933111313988 1104 78-79 1.056068397323216 1104 80-81 1.3047036035572894 1104 82-83 1.3054818826036865 1104 84-85 1.1522020698305937 1104 86-87 1.361057859509888 1104 88-89 1.4258314847212397 1104 90-91 1.5009111583233512 1104 92-93 1.6358723107600923 1104 94-95 1.542251954802584 1104 96-97 1.3811828105987196 1104 98-99 1.294924187100925 1104 100-101 1.2165175074317212 1104 102-103 1.4512645757167526 1104 104-105 1.4693732657914786 1104 106-107 1.556567162414698 1104 108-109 1.6695740887266979 1104 110-111 1.390766994637758 1104 112-113 1.1739127941053091 1104 114-115 1.2277547928839851 1104 116-117 1.4402533244063456 1104 118-119 0.9776591121559797 1104 120-121 1.244734208727067 1105 1 0.6023145820163265 1105 2 0.7700145107127305 1105 3 0.9592517965280898 1105 4 0.9837520289122423 1105 5 1.0156196410187874 1105 6 1.0155034208086917 1105 7 1.1409834596297088 1105 8 0.87102673626989 1105 9 1.0627757398105828 1105 10-11 0.8345530672142374 1105 12-13 1.0510332636512345 1105 14-15 1.2894726997709824 1105 16-17 1.1981803539974223 1105 18-19 1.150096892583747 1105 20-21 1.3399689717355727 1105 22-23 1.2756209927877364 1105 24-25 1.343466897045161 1105 26-27 1.2969068121419483 1105 28-29 1.1936237803189087 1105 30-31 1.3061691595827725 1105 32-33 1.3496300463820319 1105 34-35 1.3280378609332004 1105 36-37 0.964253007336012 1105 38-39 1.005298238361398 1105 40-41 0.9715011866512668 1105 42-43 1.015851398388044 1105 44-45 0.8351559962091173 1105 46-47 0.8048105531782284 1105 48-49 1.000071227385277 1105 50-51 0.7392359856629298 1105 52-53 0.8932415271053173 1105 54-55 0.9871129367905311 1105 56-57 0.8607087100192174 1105 58-59 1.1782007570330677 1105 60-61 0.9489945832372797 1105 62-63 1.1998362968741887 1105 64-65 1.2861199610130818 1105 66-67 1.0674840692258876 1105 68-69 1.113242472180623 1105 70-71 0.8554156480869501 1105 72-73 0.92116367287408 1105 74-75 0.6524406468083122 1105 76-77 0.8837065824754369 1105 78-79 0.932376704400756 1105 80-81 1.0537314987390296 1105 82-83 1.2616718464859566 1105 84-85 1.4572508671870814 1105 86-87 0.9324941155615285 1105 88-89 1.2202678176191135 1105 90-91 1.1742762547653705 1105 92-93 1.109437207401914 1105 94-95 1.1725754774337993 1105 96-97 1.0424676795758963 1105 98-99 0.8683507605275018 1105 100-101 0.6688036827948203 1105 102-103 0.7769158475218703 1105 104-105 1.0029704378501876 1105 106-107 1.2253522235074534 1105 108-109 1.202033936571162 1105 110-111 1.001148920404301 1105 112-113 1.2143569653187107 1105 114-115 1.1192786536386166 1105 116-117 1.1903109590793655 1105 118-119 0.7335570603616226 1105 120-121 0.6367575699811958 1106 1 0.5502505170490366 1106 2 0.7104160446626544 1106 3 0.4670117784816199 1106 4 0.6504337343036255 1106 5 0.7331472746754528 1106 6 0.8631686587965106 1106 7 0.6346897533359979 1106 8 0.8113380378715149 1106 9 0.5326494145229645 1106 10-11 0.828969940663363 1106 12-13 0.863412013933214 1106 14-15 1.1144332231186098 1106 16-17 0.7363486373269978 1106 18-19 0.7357950653786958 1106 20-21 0.8559682949929623 1106 22-23 0.7059841779896274 1106 24-25 0.6899647540268887 1106 26-27 0.7496307011561569 1106 28-29 0.8004137645282441 1106 30-31 0.8213845893143272 1106 32-33 0.36944732587672746 1106 34-35 0.49685694507486033 1106 36-37 0.2924506161787761 1106 38-39 0.43537944747485824 1106 40-41 0.2783532056000624 1106 42-43 0.44990283082657356 1106 44-45 0.5312760052323497 1106 46-47 0.29421953129688205 1106 48-49 0.5345005077823046 1106 50-51 0.30257909416732787 1106 52-53 0.4876471215109106 1106 54-55 0.67470598889971 1106 56-57 0.7487980400440293 1106 58-59 0.7234748377910236 1106 60-61 0.43590525321246076 1106 62-63 0.164995331388063 1106 64-65 0.27724899327114727 1106 66-67 0.5778777078453352 1106 68-69 0.6219950099654206 1106 70-71 0.3733662458762588 1106 72-73 0.2543691770473231 1106 74-75 0.3887591669977972 1106 76-77 0.8830242003414419 1106 78-79 0.5791678165144454 1106 80-81 0.3405180992353074 1106 82-83 0.522855018152999 1106 84-85 0.4428757261990377 1106 86-87 0.49291031226804805 1106 88-89 0.5999711787740871 1106 90-91 0.7306094377114576 1106 92-93 0.5439116039730827 1106 94-95 0.6807189468675929 1106 96-97 0.7617604835461194 1106 98-99 0.9230259240736309 1106 100-101 0.5045018555897656 1106 102-103 0.07155266231997714 1106 104-105 0.1464285925986637 1106 106-107 -0.04020157753021891 1106 108-109 -0.48270551752313295 1106 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-0.9473706530143495 2316 92-93 -1.151974232720466 2316 94-95 -0.8145557507130547 2316 96-97 -0.8262372483087184 2316 98-99 -0.650688793668305 2316 100-101 -0.6833761686037825 2316 102-103 -0.35935081550260506 2316 104-105 -0.28556485498198114 2316 106-107 -0.5857723940624773 2316 108-109 -0.5141319288134554 2316 110-111 -0.7678810730397565 2316 112-113 -0.6249145053980705 2316 114-115 -0.6862119495082375 2316 116-117 -0.9265965474765778 2316 118-119 -0.7056638749530642 2316 120-121 -1.0710289794069148 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11389.0 3 1593.0 4 1386.0 5 1571.0 6 1895.0 7 2074.0 8 2708.0 9 3315.0 10 4586.0 11 5740.0 12 6151.0 13 6865.0 14 8520.0 15 8859.0 16 7315.0 17 7509.0 18 7914.0 19 8638.0 20 9308.0 21 10047.0 22 11238.0 23 12216.0 24 13822.0 25 15396.0 26 17269.0 27 19884.0 28 22912.0 29 26465.0 30 31017.0 31 36863.0 32 44402.0 33 53702.0 34 66494.0 35 83901.0 36 105367.0 37 108584.0 38 36577.0 39 186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.872281961567346 17.43635775217229 13.473166144583463 21.218194141676893 2 13.336963506943933 22.086908318493773 36.804441566468974 27.771686608093326 3 24.676905741720557 23.871497158534197 31.126298255927885 20.325298843817365 4 10.443697627195034 14.996044100320635 44.06198637602847 30.498271896455858 5 10.337187369865026 38.899884868823946 35.96477475932685 14.798153001984177 6 25.462161202219256 35.79198765447219 19.16937133339049 19.576479809918062 7 23.449188953502613 33.019341658542324 24.743164526575185 18.78830486137988 8 21.657535572074405 27.160933858049756 21.955291319274757 29.226239250601083 9 20.295479127698382 20.729229061858057 28.70846394216341 30.266827868280156 10-11 24.347882009946836 22.42429624911189 28.67150942009457 24.556312320846708 12-13 29.14085646680644 21.824327097233784 27.403924191668338 21.630892244291434 14-15 23.41478951962391 23.886620829058387 32.03944688605424 20.659142765263464 16-17 19.102942005228815 28.124793260855185 31.22845318690689 21.543811547009113 18-19 17.449428449441314 32.20113581163487 29.61900508977396 20.730430649149856 20-21 20.35315666311366 27.059825290060157 26.43786525526519 26.149152791560997 22-23 18.181428364905162 26.729957340664562 29.38667097915549 25.70194331527479 24-25 23.449047754405914 25.58247240791202 27.804725941463605 23.163753896218463 26-27 21.525881218519157 22.797796050092444 34.430407353361 21.245915378027398 28-29 18.318109695956085 30.110455606403118 30.49291503556266 21.078519662078133 30-31 19.344743204378997 26.283304148995747 29.173840213607292 25.19811243301796 32-33 20.7846071082387 25.790127677721287 31.04631028664071 22.378954927399302 34-35 24.186674918123693 24.252727423125116 29.290058117626156 22.270539541125036 36-37 21.949482905045457 23.457140371151507 33.88022808795578 20.713148635847258 38-39 21.30044856787308 27.11056974430835 31.52909502764259 20.059886660175984 40-41 19.758746171242795 29.20559759861328 30.596664209644636 20.438992020499285 42-43 18.427081259094738 28.71832733399709 26.732695106073372 26.121896300834802 44-45 19.986200988847433 25.878476790149023 27.181539717193804 26.95378250380974 46-47 23.371320235838247 27.636543898586126 28.603782186638693 20.388353678936937 48-49 22.065036953789534 26.107434081559994 29.414799776702417 22.41272918794806 50-51 17.071407194442266 27.621317901719127 33.979315487871894 21.327959415966717 52-53 19.651683210427194 29.674952948797383 27.842790456526895 22.830573384248527 54-55 18.036275031315956 26.198034510061554 30.25752624779685 25.50816421082564 56-57 22.156195331480795 23.996151948994942 28.617612186311845 25.230040533212417 58-59 25.464888338000847 24.503726149306853 27.903282404087403 22.128103108604897 60-61 22.384325219470085 25.2505319419419 32.96159173938429 19.403551099203725 62-63 19.67549543033327 27.341256949509933 31.43409688551621 21.549150734640588 64-65 18.09820707815217 31.492806185338264 29.000576780095997 21.408409956413568 66-67 19.738551995370777 27.0949186453644 26.3220128284344 26.844516530830415 68-69 18.37348658928663 27.417165516920033 28.84848358514951 25.360864308643823 70-71 23.1017907408737 26.01609402356112 26.939969279843684 23.942145955721493 72-73 20.97154180420776 23.700700761515865 33.01305359331285 22.314703840963528 74-75 18.480791725326398 30.026622414624775 29.571316526885695 21.92126933316313 76-77 19.32009182773647 26.321005564034262 28.62400150223402 25.73490110599525 78-79 20.783979637083437 25.90747514043602 30.004066703740634 23.30447851873991 80-81 23.581398937649038 25.078108638062147 28.209557729356476 23.13093469493234 82-83 20.854417793128892 24.181804476350575 32.770229896962185 22.19354783355835 84-85 20.784914715419852 26.839519396451355 30.080554037260992 22.295011850867795 86-87 20.045801395735335 28.6943231762171 29.22201359457002 22.03786183347755 88-89 18.927760298376846 27.092737788966776 26.529236494047538 27.450265418608844 90-91 19.486846637468297 24.182672447502387 28.252670081723565 28.077810833305755 92-93 22.19996126634536 25.908586123545653 29.25861823815804 22.63283437195095 94-95 20.65538345000202 24.68820594158435 30.26973326975901 24.386677338654618 96-97 15.816723642777559 26.622838574767815 34.03067271851432 23.52976506394031 98-99 18.777032166170944 28.626929905903392 28.442205634764445 24.15383229316122 100-101 16.838267876854093 25.462783917312713 30.476043964395533 27.22290424143766 102-103 20.481624978703532 23.6386501165024 28.90540446128823 26.974320443505835 104-105 23.408032779752155 24.294449050981807 28.877534948910334 23.419983220355707 106-107 20.910776796132687 25.10663541962876 32.08770089132503 21.894886892913526 108-109 19.640815005754757 26.110287835831965 30.531398656856002 23.717498501557277 110-111 18.5487597551992 29.091484065026897 28.04576103117374 24.313995148600167 112-113 20.49914412998193 25.427153602468138 25.584599365191433 28.4891029023585 114-115 18.903093743871345 24.870685428515397 28.275764855854092 27.95045597175917 116-117 22.969714724892714 24.302379601107614 26.386507135861457 26.341398538138215 118-119 21.422529980031698 23.092929211209213 30.518526145725694 24.96601466303339 120-121 18.769700524448414 28.150812168772244 27.721422709590904 25.358064597188434 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2172.0 1 2115.5 2 1429.0 3 772.5 4 677.0 5 615.0 6 599.5 7 591.0 8 686.0 9 775.5 10 744.0 11 691.5 12 717.0 13 778.5 14 842.5 15 913.5 16 961.5 17 1041.0 18 1145.0 19 1249.5 20 1402.5 21 1553.5 22 1790.0 23 2094.0 24 2461.0 25 2998.5 26 3753.0 27 4818.0 28 6186.5 29 7786.5 30 9339.5 31 10769.5 32 12374.0 33 13769.5 34 14917.5 35 16182.5 36 17354.0 37 18786.0 38 20104.5 39 20660.5 40 21190.5 41 22009.0 42 22532.5 43 23348.0 44 24062.5 45 25239.5 46 30105.5 47 52129.5 48 100704.0 49 101167.0 50 54519.5 51 30952.0 52 20953.5 53 16234.5 54 13664.5 55 11565.5 56 9870.0 57 8147.0 58 6619.5 59 5542.0 60 4461.0 61 3425.0 62 2518.0 63 1797.0 64 1241.0 65 818.5 66 507.0 67 300.0 68 177.0 69 100.5 70 67.5 71 46.0 72 28.0 73 16.5 74 16.0 75 14.0 76 11.5 77 8.5 78 4.5 79 4.5 80 5.5 81 4.5 82 3.5 83 1.5 84 0.5 85 1.0 86 1.0 87 1.0 88 1.5 89 1.5 90 0.5 91 0.0 92 1.0 93 1.5 94 0.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5879724843931731 2 1.0064612627750165 3 0.873885183287644 4 0.871214236631305 5 0.8763133166115884 6 0.8726711166256717 7 0.8743708099524329 8 0.8786200432693357 9 0.8873613232355362 10-11 0.8908821165552558 12-13 0.8962847132010324 14-15 0.8972559665306103 16-17 0.9022943431777952 18-19 0.902476453177091 20-21 0.9066649831608955 22-23 0.9037512231721618 24-25 0.9107928098116012 26-27 0.9113998431425873 28-29 0.9121282831397706 30-31 0.9228120697651266 32-33 0.9274255230806213 34-35 0.9300357664038617 36-37 0.932038976396116 38-39 0.9271220064151282 40-41 0.9287002930756922 42-43 0.9245724664249866 44-45 0.9320996797292145 46-47 0.9342242963876661 48-49 0.9380486063728787 50-51 1.0176306760651614 52-53 0.948368172999643 54-55 0.9469719963383749 56-57 0.9324638997278062 58-59 0.8763740199446871 60-61 0.9183807264489278 62-63 0.9679753495904954 64-65 0.9642724462714799 66-67 0.9700392629158483 68-69 0.9877646361806434 70-71 1.0809442524870154 72-73 1.0831295724785657 74-75 1.086711069131384 76-77 1.0794873724926488 78-79 0.8852974099101834 80-81 0.8725497099594745 82-83 0.9484895796658402 84-85 0.9353169563834411 86-87 0.9426013563552749 88-89 0.9466077763397832 90-91 0.956563122967956 92-93 0.9530423296482362 94-95 0.951221229655278 96-97 0.9517068563200668 98-99 0.9551669463066879 100-101 0.956866639633449 102-103 0.9487930963313334 104-105 0.9480039530010513 106-107 0.9486716896651362 108-109 0.9507356029904891 110-111 0.9507356029904891 112-113 0.9520103729855598 114-115 0.9515854496538696 116-117 0.9553490563059837 118-119 0.9572915629651393 120-121 0.9552883529728851 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 823678.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.82384327102543 #Duplication Level Percentage of deduplicated Percentage of total 1 88.2754843163164 50.16152285464226 2 7.592001612693452 8.6281341950613 3 1.6623581537225474 2.833847375623237 4 0.7267588025589057 1.651889131697815 5 0.41554143133748944 1.1806330583469538 6 0.26940387696120244 0.9185138208629993 7 0.1907988277420082 0.7589345878735078 8 0.13908557442266622 0.6322701505803304 9 0.10435491115249695 0.5336862404302143 >10 0.5561742423725295 5.544016888009336 >50 0.03380525514014799 1.342592902290462 >100 0.023535184462071174 2.921522907716724 >500 0.0034232995578012304 1.3634989350228333 >1k 0.005562861781426999 7.430005387075382 >5k 8.558248894503076E-4 3.400297656238841 >10k+ 8.558248894503076E-4 10.698633908527801 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 30540 3.7077595856633296 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 23617 2.867261235579923 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 18817 2.2845092378332312 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 15024 1.8240137529471467 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 9593 1.164654148830004 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6715 0.815245763514383 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 6500 0.7891433302819791 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 5160 0.6264583975776942 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 4201 0.510029404694553 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4189 0.5085725247001862 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3981 0.48331993813116286 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3969 0.48186305813679614 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3710 0.4504187315917142 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3678 0.446533718273403 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3281 0.3983352717931037 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3140 0.3812169318592945 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 3059 0.3713829918973191 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2997 0.363855778593091 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2301 0.2793567389198206 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2081 0.25264727235643053 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1879 0.2281231257845906 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1843 0.22375248580149038 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1714 0.208091025862048 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1637 0.1987427125648615 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1615 0.19607176590852252 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1447 0.17567544598738827 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1447 0.17567544598738827 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1397 0.1696051126775269 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1390 0.1687552660141463 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1330 0.16147086604231264 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1322 0.16049961271273483 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1218 0.14787331942822315 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1170 0.14204579945075624 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1117 0.1356112461423032 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 954 0.1158219595521551 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 940 0.11412226622539391 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 926 0.11242257289863272 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 825 0.10016049961271273 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 1.2140666619722756E-4 0.0 0.0 0.0 9 0.0 1.2140666619722756E-4 0.0 0.0 0.0 10-11 0.0 1.2140666619722756E-4 0.0 1.2140666619722756E-4 0.0 12-13 0.0 1.2140666619722756E-4 0.0 2.4281333239445512E-4 0.0 14-15 0.0 1.2140666619722756E-4 0.0 4.2492333169029644E-4 0.0 16-17 0.0 1.2140666619722756E-4 0.0 7.284399971833654E-4 0.0 18-19 0.0 1.2140666619722756E-4 0.0 9.105499964792067E-4 0.0 20-21 0.0 1.2140666619722756E-4 0.0 0.0014568799943667308 0.0 22-23 0.0 1.2140666619722756E-4 0.0 0.0029137599887334616 0.0 24-25 0.0 1.2140666619722756E-4 0.0 0.00497767331408633 0.0 26-27 0.0 1.2140666619722756E-4 0.0 0.0075272133042281085 0.0 28-29 0.0 1.2140666619722756E-4 0.0 0.015054426608456217 0.0 30-31 0.0 1.2140666619722756E-4 0.0 0.029805336551419363 0.0 32-33 0.0 1.2140666619722756E-4 0.0 0.05554354978523161 0.0 34-35 0.0 1.2140666619722756E-4 0.0 0.07509002304298523 0.0 36-37 0.0 1.2140666619722756E-4 0.0 0.09311891297327354 0.0 38-39 0.0 1.2140666619722756E-4 0.0 0.11667180621553569 0.0 40-41 0.0 1.2140666619722756E-4 0.0 0.1405282161232909 0.0 42-43 0.0 1.2140666619722756E-4 0.0 0.20857665252683694 0.0 44-45 0.0 1.2140666619722756E-4 0.0 0.2762608689317913 0.0 46-47 0.0 1.2140666619722756E-4 0.0 0.32621971207195044 0.0 48-49 0.0 1.2140666619722756E-4 0.0 0.37642136854450403 0.0 50-51 0.0 1.2140666619722756E-4 0.0 0.44125252829382355 0.0 52-53 0.0 1.2140666619722756E-4 0.0 0.5968351710255706 0.0 54-55 0.0 1.2140666619722756E-4 0.0 0.6816377273643341 0.0 56-57 0.0 1.2140666619722756E-4 0.0 0.9478825463348541 0.0 58-59 0.0 1.2140666619722756E-4 0.0 1.219833478616644 0.0 60-61 0.0 1.2140666619722756E-4 0.0 1.3921095379505097 0.0 62-63 0.0 1.2140666619722756E-4 0.0 1.7057636600710473 0.0 64-65 0.0 1.2140666619722756E-4 0.0 1.833908396242221 0.0 66-67 0.0 1.2140666619722756E-4 0.0 2.3469729675917046 0.0 68-69 0.0 1.2140666619722756E-4 0.0 2.8271363324017393 0.0 70-71 0.0 1.2140666619722756E-4 0.0 2.993402761758843 0.0 72-73 0.0 1.8210999929584135E-4 0.0 3.3793545535998293 0.0 74-75 0.0 2.4281333239445512E-4 0.0 3.693979929049944 0.0 76-77 0.0 2.4281333239445512E-4 0.0 3.8022746752978716 0.0 78-79 0.0 2.4281333239445512E-4 0.0 3.9644739813373677 0.0 80-81 0.0 2.4281333239445512E-4 0.0 4.309208210004394 0.0 82-83 0.0 2.4281333239445512E-4 0.0 4.534174762467858 0.0 84-85 0.0 2.4281333239445512E-4 0.0 5.086939313663835 0.0 86-87 0.0 2.4281333239445512E-4 0.0 6.302098635631886 0.0 88-89 0.0 2.4281333239445512E-4 0.0 7.942666915955021 0.0 90-91 0.0 2.4281333239445512E-4 0.0 9.316626157309045 0.0 92-93 0.0 2.4281333239445512E-4 0.0 10.398359553126344 0.0 94-95 0.0 2.4281333239445512E-4 0.0 11.20565318000481 0.0 96-97 0.0 2.4281333239445512E-4 0.0 12.1868618562108 0.0 98-99 0.0 2.4281333239445512E-4 0.0 13.152530479143548 0.0 100-101 0.0 2.4281333239445512E-4 0.0 13.687084030409942 0.0 102-103 0.0 2.4281333239445512E-4 0.0 14.131371725358695 0.0 104-105 0.0 2.4281333239445512E-4 0.0 14.577237706968013 0.0 106-107 0.0 2.4281333239445512E-4 1.2140666619722756E-4 15.202542741216835 0.0 108-109 0.0 2.4281333239445512E-4 1.2140666619722756E-4 16.02695470802911 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 115 0.0 80.06535 2 TACCTGG 1145 0.0 66.844955 2 ACCTGGG 1250 0.0 60.63548 3 GTACCCG 115 0.0 55.153378 1 GTACCTG 1520 0.0 51.97018 1 ATAACGC 215 0.0 48.07253 3 TAACGCA 225 0.0 43.38929 4 TACCCGG 140 5.820766E-11 41.10498 2 AGTACGC 400 0.0 40.282883 2 GTATAAC 255 0.0 38.44023 1 GTACGCA 475 0.0 35.056396 3 CCTGGGG 870 0.0 34.98427 4 GAGTACG 475 0.0 33.989258 1 TACGCAG 495 0.0 33.6441 4 ACGCAGT 615 0.0 27.079401 5 CCTGGGA 730 0.0 25.960154 4 GACGACC 315 0.0 25.539555 114-115 GTAGGCA 480 0.0 25.121231 3 CGCAGTG 665 0.0 25.043354 6 GGCACCG 115 0.0012371301 24.962141 9 >>END_MODULE