##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003844_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 823678 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.61897853287328 31.0 31.0 34.0 30.0 34.0 2 31.807255990811942 31.0 31.0 34.0 30.0 34.0 3 31.888137111832535 31.0 31.0 34.0 30.0 34.0 4 35.527664937026366 37.0 35.0 37.0 33.0 37.0 5 35.462969752743184 37.0 35.0 37.0 33.0 37.0 6 35.542621024235196 37.0 35.0 37.0 33.0 37.0 7 35.497420108343306 37.0 35.0 37.0 33.0 37.0 8 35.50575467597775 37.0 35.0 37.0 33.0 37.0 9 37.03946202278075 39.0 37.0 39.0 33.0 39.0 10-11 36.947934751201316 39.0 37.0 39.0 33.0 39.0 12-13 36.91284822467032 39.0 37.0 39.0 33.0 39.0 14-15 37.81764051000512 39.0 37.0 41.0 32.5 41.0 16-17 37.98264248893378 39.5 37.0 41.0 33.0 41.0 18-19 38.052774263729276 40.0 37.0 41.0 33.0 41.0 20-21 37.95142944208781 40.0 37.0 41.0 32.5 41.0 22-23 37.83918169478849 39.5 37.0 41.0 32.5 41.0 24-25 37.654516692202535 39.5 36.5 41.0 32.0 41.0 26-27 37.297672148582336 39.0 36.0 40.5 31.5 41.0 28-29 37.22101355141208 39.0 36.0 40.0 31.0 41.0 30-31 37.08293289367933 39.0 36.0 40.0 31.0 41.0 32-33 36.80052884743795 39.0 35.0 40.0 30.0 41.0 34-35 36.634542867479766 38.5 35.0 40.0 30.0 41.0 36-37 36.368134149509885 38.5 35.0 40.0 30.0 41.0 38-39 36.1407321793225 38.0 35.0 40.0 30.0 41.0 40-41 36.53344207323735 39.0 35.0 40.0 30.0 41.0 42-43 36.40478925016815 39.0 35.0 40.0 30.0 41.0 44-45 36.219311429952 39.0 35.0 40.0 30.0 41.0 46-47 35.931714820597364 39.0 35.0 40.0 28.5 41.0 48-49 35.57300122135106 38.5 35.0 40.0 27.5 41.0 50-51 35.196880941338726 38.0 34.0 40.0 27.0 41.0 52-53 35.17250369683298 38.0 34.0 40.0 26.5 41.0 54-55 34.98121292543931 38.0 34.0 40.0 26.0 41.0 56-57 34.58551278533602 38.0 33.5 40.0 25.0 41.0 58-59 34.26454816081041 38.0 33.0 40.0 24.0 41.0 60-61 33.57728141336784 37.0 32.0 40.0 22.0 41.0 62-63 33.53916032721524 37.0 32.0 40.0 22.0 41.0 64-65 33.105312391492795 36.0 32.0 39.0 22.0 40.5 66-67 32.63769579860091 36.0 31.0 39.0 21.0 40.0 68-69 32.20752223563092 35.0 31.0 38.0 20.0 40.0 70-71 31.600961176576284 35.0 30.5 37.5 18.5 40.0 72-73 31.10724943485197 34.5 30.0 37.0 16.0 39.0 74-75 30.801348342434785 34.0 30.0 36.5 15.0 39.0 76-77 29.87646750307766 33.5 29.0 35.0 12.5 38.0 78-79 30.249443957468817 34.0 30.0 36.0 11.5 37.5 80-81 29.88422903124765 34.0 29.0 35.0 7.0 37.0 82-83 29.520073378189046 34.0 29.0 35.0 7.0 37.0 84-85 29.2150251676019 34.0 29.0 35.0 2.0 36.0 86-87 29.095870595062635 34.0 29.0 35.0 2.0 36.0 88-89 28.752103370491866 34.0 29.0 35.0 2.0 36.0 90-91 28.348028598554286 34.0 29.0 35.0 2.0 35.0 92-93 28.08249704374768 34.0 28.0 35.0 2.0 35.0 94-95 27.65675968036053 33.0 27.0 35.0 2.0 35.0 96-97 27.12998404716406 33.0 25.5 35.0 2.0 35.0 98-99 26.884205963981067 33.0 25.5 35.0 2.0 35.0 100-101 25.77774385135939 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1463065695191368 1101 2 0.23763428880664605 1101 3 0.28880920413405775 1101 4 0.22127541908768933 1101 5 0.2132044200439509 1101 6 0.2594350030459367 1101 7 0.26129539361514986 1101 8 0.2910941043532489 1101 9 0.30227940766877737 1101 10-11 0.2893818727093773 1101 12-13 0.3664096719596799 1101 14-15 0.4966118487350215 1101 16-17 0.42621457763739556 1101 18-19 0.18447164155974605 1101 20-21 0.2210618963962716 1101 22-23 0.34656481767135716 1101 24-25 0.4243763930155353 1101 26-27 0.48876753952226437 1101 28-29 0.319075416035254 1101 30-31 0.520063259674437 1101 32-33 0.4530070685087182 1101 34-35 0.5012139690777317 1101 36-37 0.5901118433028003 1101 38-39 0.43140152568764023 1101 40-41 0.5117750456273527 1101 42-43 0.48437905872732756 1101 44-45 0.44210875833420715 1101 46-47 0.5298666607556513 1101 48-49 0.47540364460430595 1101 50-51 0.650196332008953 1101 52-53 0.7262547866291484 1101 54-55 0.6962262693737031 1101 56-57 0.7518565457941477 1101 58-59 0.8247548447459394 1101 60-61 0.8510963190242222 1101 62-63 0.7489903848921031 1101 64-65 0.7694192985638679 1101 66-67 0.5983746262723315 1101 68-69 0.6808785423406398 1101 70-71 0.7773353307873165 1101 72-73 0.8575807256378951 1101 74-75 0.707418892414335 1101 76-77 0.8203227111711868 1101 78-79 0.7984952593495862 1101 80-81 0.8132525646343325 1101 82-83 0.5546407015776644 1101 84-85 0.44378063085605035 1101 86-87 0.4551881426742135 1101 88-89 0.627776136463563 1101 90-91 0.7980119874307086 1101 92-93 0.7856784567019659 1101 94-95 0.890795711423678 1101 96-97 1.1031306172227815 1101 98-99 0.7738527090324148 1101 100-101 0.9510147603576122 1102 1 0.035037157986913314 1102 2 0.14851039284963363 1102 3 0.13145979974128963 1102 4 0.18372543607121372 1102 5 0.09208410838487424 1102 6 0.19378388746052622 1102 7 -0.026355898635351593 1102 8 0.1284875475285574 1102 9 0.22636395921718844 1102 10-11 0.12770303527496196 1102 12-13 0.20376464039217268 1102 14-15 0.3750132208946013 1102 16-17 0.26753950207664445 1102 18-19 0.13119345687551487 1102 20-21 0.29070004257843607 1102 22-23 0.17821482409140543 1102 24-25 0.35851947170526444 1102 26-27 0.20399843806458762 1102 28-29 0.32993644890679974 1102 30-31 0.3151925393635082 1102 32-33 0.33285545525592397 1102 34-35 0.12847771822058718 1102 36-37 -0.07596563617808982 1102 38-39 -0.07373519396944772 1102 40-41 0.11026219142068072 1102 42-43 0.28865462712904844 1102 44-45 0.13069578364922307 1102 46-47 0.1874671754520918 1102 48-49 -0.030905985555520488 1102 50-51 0.19797012419798676 1102 52-53 0.30242020707840567 1102 54-55 0.39517574953212176 1102 56-57 0.40484532756085656 1102 58-59 0.310516457971417 1102 60-61 0.38897641078078493 1102 62-63 0.2758865067290941 1102 64-65 0.2759789007213769 1102 66-67 0.3769199258425999 1102 68-69 0.39379412647830137 1102 70-71 0.5433324003323534 1102 72-73 0.6188720381775639 1102 74-75 0.6675932080419997 1102 76-77 0.6161902961320749 1102 78-79 0.7107302730957699 1102 80-81 0.473138570118234 1102 82-83 0.394545930773301 1102 84-85 0.3590121213175017 1102 86-87 0.3583781769417804 1102 88-89 0.3699725935089013 1102 90-91 0.5498870050744564 1102 92-93 0.9199764816044578 1102 94-95 0.9367894206893972 1102 96-97 0.9214606221493717 1102 98-99 0.6132484324623064 1102 100-101 0.7557677342958655 1103 1 0.07663758194027892 1103 2 0.10120290436567458 1103 3 0.10237415300858288 1103 4 0.11499607322420502 1103 5 0.029734428388138667 1103 6 0.12262902218801486 1103 7 0.06848328443912521 1103 8 0.07041816592976602 1103 9 -0.06286191824398202 1103 10-11 0.09568232678366684 1103 12-13 0.16245976085199487 1103 14-15 0.2469507693027353 1103 16-17 0.07026870186466283 1103 18-19 -0.022194093570853113 1103 20-21 0.1554426132033626 1103 22-23 0.2005742876273402 1103 24-25 0.0206001043741324 1103 26-27 0.3483464059419745 1103 28-29 0.22134935502559472 1103 30-31 0.3674325621917589 1103 32-33 0.22676520210684714 1103 34-35 0.19972430417305276 1103 36-37 0.2835998116226506 1103 38-39 0.2775142455152917 1103 40-41 0.409281799263816 1103 42-43 0.4492983024786099 1103 44-45 0.500747962524926 1103 46-47 0.7872490840575281 1103 48-49 0.8448736402241082 1103 50-51 0.8428072690351343 1103 52-53 0.8482092087497577 1103 54-55 0.8804291024325366 1103 56-57 1.017865587639939 1103 58-59 0.8958880286371027 1103 60-61 1.0446264046660758 1103 62-63 0.8313163709824849 1103 64-65 0.8147563659694228 1103 66-67 0.7861470262461694 1103 68-69 0.8733913707814303 1103 70-71 0.8272180959827509 1103 72-73 1.0998960486133385 1103 74-75 1.0124018175564906 1103 76-77 1.0908244325309155 1103 78-79 1.094642465831491 1103 80-81 1.2628128597925254 1103 82-83 1.1451166372263586 1103 84-85 0.9819280236451675 1103 86-87 0.9175278243267222 1103 88-89 0.9709366797671031 1103 90-91 1.1029627052089772 1103 92-93 1.1705777616175048 1103 94-95 0.8781595238242055 1103 96-97 1.5123048600524314 1103 98-99 1.4076004768143484 1103 100-101 1.4995205368721969 1104 1 -0.08976388989105644 1104 2 -0.01262369136137309 1104 3 0.027547772752338773 1104 4 -0.044949561834549456 1104 5 0.09889226903919024 1104 6 0.023968256106435604 1104 7 0.004745363235137745 1104 8 -0.17046990527504846 1104 9 -0.350631463932082 1104 10-11 -0.09087395253279595 1104 12-13 -0.08860930582792292 1104 14-15 9.9084687222728E-4 1104 16-17 -0.05312562628079576 1104 18-19 -0.1373176577894455 1104 20-21 -0.1469375104437347 1104 22-23 -0.06641061284172167 1104 24-25 -0.04075935065048242 1104 26-27 -0.031081104707261886 1104 28-29 -0.1947869219704117 1104 30-31 -0.09305340734398015 1104 32-33 -0.08693542607342408 1104 34-35 0.10198757634582734 1104 36-37 0.2580416593676631 1104 38-39 0.12910458579341366 1104 40-41 0.0327755754725203 1104 42-43 0.26224063917659635 1104 44-45 0.18115918026645517 1104 46-47 0.14489332518594011 1104 48-49 0.1444650744309257 1104 50-51 0.41550380327013414 1104 52-53 0.42445686757660184 1104 54-55 0.15974970641376984 1104 56-57 0.30296661029752414 1104 58-59 0.5463156553090727 1104 60-61 0.7252530932113217 1104 62-63 0.489186027720919 1104 64-65 0.5154715632909586 1104 66-67 0.5059894010659178 1104 68-69 0.392659433035913 1104 70-71 0.6369408016330596 1104 72-73 0.6115231846748657 1104 74-75 0.49081330049286365 1104 76-77 0.5231873185137097 1104 78-79 0.7270119816851697 1104 80-81 0.7568957000292116 1104 82-83 0.5986536733425787 1104 84-85 0.7487678956886619 1104 86-87 0.6759933407107894 1104 88-89 0.6286903137650803 1104 90-91 0.6471507946459347 1104 92-93 0.5590716206995978 1104 94-95 0.45182982034518204 1104 96-97 0.7769775930509546 1104 98-99 0.6488656219256477 1104 100-101 0.9231692863128025 1105 1 -0.13138380843405173 1105 2 -0.06902882468958182 1105 3 -0.04783837955688952 1105 4 0.02839248073826184 1105 5 0.0352328515336211 1105 6 0.059102352778992895 1105 7 -0.13709740937686377 1105 8 0.15972297876398756 1105 9 -0.02348169062845784 1105 10-11 0.13761294518486977 1105 12-13 0.011513648141182387 1105 14-15 0.07611572199710537 1105 16-17 0.0966707312078654 1105 18-19 0.030891056573118192 1105 20-21 -0.11437776249936604 1105 22-23 -0.029893284016701216 1105 24-25 -0.00745419426840499 1105 26-27 0.06766630173245147 1105 28-29 -0.003900884930530424 1105 30-31 -0.28183385766289604 1105 32-33 -0.14870442630395786 1105 34-35 0.06421765396051882 1105 36-37 0.18384662363416737 1105 38-39 0.15491723468298346 1105 40-41 -0.014446433287943705 1105 42-43 0.25481729329171543 1105 44-45 -0.00992665697322792 1105 46-47 0.0320523200372449 1105 48-49 -0.03636179101132342 1105 50-51 -0.004045877818008137 1105 52-53 0.127223331881531 1105 54-55 0.17597963033599484 1105 56-57 -0.3022358795203459 1105 58-59 -0.24925800180304236 1105 60-61 -0.08461396280958411 1105 62-63 -0.2914794606368716 1105 64-65 -0.23311830837583614 1105 66-67 -0.2360454871228157 1105 68-69 -0.32656749388332074 1105 70-71 -0.12664176964181806 1105 72-73 -0.27181655251102654 1105 74-75 -0.44117010072130824 1105 76-77 -0.5419398619980846 1105 78-79 -0.42139345902796776 1105 80-81 -0.10501930803964399 1105 82-83 -0.04952891330154685 1105 84-85 -0.21857245626707567 1105 86-87 0.06882358738718963 1105 88-89 -0.07397625043994438 1105 90-91 0.014329769517217983 1105 92-93 -0.03913017750220149 1105 94-95 -0.012413628513652242 1105 96-97 0.23252973321848103 1105 98-99 -0.027481108267235044 1105 100-101 0.21926549779363214 1106 1 -0.06610070444127203 1106 2 -0.18979354384140024 1106 3 -0.09015735090811816 1106 4 0.06191379813054709 1106 5 -0.05787565286520646 1106 6 -0.16730188814137392 1106 7 -0.05421899746619374 1106 8 0.16889284115966063 1106 9 -0.0834185279846551 1106 10-11 -0.17572452379776138 1106 12-13 -0.4309463112542673 1106 14-15 -0.19806090782469 1106 16-17 -0.10321083883498972 1106 18-19 0.06551208071027048 1106 20-21 -0.08225504650567927 1106 22-23 -0.2985657405923874 1106 24-25 -0.23388099327583944 1106 26-27 -0.18699758277013956 1106 28-29 -0.303105712361166 1106 30-31 -0.10967053711247132 1106 32-33 0.08031091319524819 1106 34-35 -0.2868482156537411 1106 36-37 -0.34669138674255606 1106 38-39 -0.12668100578621733 1106 40-41 -0.2847543511765096 1106 42-43 -0.07614932073941816 1106 44-45 -0.07558760892450067 1106 46-47 -0.181104684248794 1106 48-49 0.15992740366496605 1106 50-51 0.13009014744704217 1106 52-53 -0.05005503491296537 1106 54-55 -0.022345431698738594 1106 56-57 -0.12664372973464566 1106 58-59 -0.34723341348813364 1106 60-61 -0.2570254223178168 1106 62-63 0.2512793934123039 1106 64-65 0.0664361694044473 1106 66-67 0.1538597689114738 1106 68-69 0.1755078501275058 1106 70-71 -0.10758582558587193 1106 72-73 0.18103140598209322 1106 74-75 0.036644020641201536 1106 76-77 -0.1090952872180253 1106 78-79 -0.06725968957161399 1106 80-81 -0.27360153226702977 1106 82-83 -0.2985363574702795 1106 84-85 -0.0868952624931083 1106 86-87 -0.20829687279778497 1106 88-89 -0.2857290138056108 1106 90-91 -0.3188418975254166 1106 92-93 -0.38368803899554393 1106 94-95 -0.28344340518858857 1106 96-97 -0.22964035475805744 1106 98-99 -0.19763111954627632 1106 100-101 -0.2626090792422353 1107 1 -0.22901300850227813 1107 2 -0.13418318252686845 1107 3 -0.06694122792803014 1107 4 -0.20994966492095557 1107 5 -0.0820449925734934 1107 6 -0.19199776661380952 1107 7 -0.15435819736935485 1107 8 -0.14793521669908216 1107 9 -0.18554889172005318 1107 10-11 -0.009291370012128652 1107 12-13 -0.1326787448317006 1107 14-15 -0.20780036191897722 1107 16-17 -0.24271183744299663 1107 18-19 -0.03254063368783733 1107 20-21 -0.2755068772870146 1107 22-23 -0.21225726150262858 1107 24-25 -0.1545461267261885 1107 26-27 -0.1643821376330621 1107 28-29 -0.025817992213490015 1107 30-31 -0.09070303726377915 1107 32-33 -0.20775269510832572 1107 34-35 -0.17439738953257233 1107 36-37 -0.022105960367198918 1107 38-39 0.25948827340037184 1107 40-41 0.30194449285907865 1107 42-43 -0.006124204235149477 1107 44-45 -0.02747739891420764 1107 46-47 0.09079357877850214 1107 48-49 0.06641835567126009 1107 50-51 0.14580914520774257 1107 52-53 0.11159993089227527 1107 54-55 0.11650495866620503 1107 56-57 -0.04886729688347202 1107 58-59 -0.34412411130330156 1107 60-61 -0.20651401397792313 1107 62-63 -0.3907801599375773 1107 64-65 -0.2561611192235276 1107 66-67 -0.16592779993195705 1107 68-69 -0.21164339359336637 1107 70-71 -0.5362443621712387 1107 72-73 -0.5883098153457524 1107 74-75 -0.42191374731861586 1107 76-77 -0.4125692326274546 1107 78-79 -0.4385860225619922 1107 80-81 -0.15275937285288066 1107 82-83 -0.30881596771055086 1107 84-85 -0.14983631436020062 1107 86-87 -0.21557006773817378 1107 88-89 -0.41197727380681926 1107 90-91 -0.4346895038922973 1107 92-93 -0.2602797596273909 1107 94-95 -0.2850215084386072 1107 96-97 -0.19295210201945068 1107 98-99 -0.1475902674007834 1107 100-101 -0.6267348433286628 1108 1 -0.3586239411826604 1108 2 -0.358779328423136 1108 3 -0.2695996691147897 1108 4 -0.20373683062833692 1108 5 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2316 78-79 0.29144116728322444 2316 80-81 -0.005065745976708058 2316 82-83 -0.06919537964769873 2316 84-85 0.13460171331334436 2316 86-87 0.45758014333124564 2316 88-89 0.36998545192019705 2316 90-91 0.25956032021651865 2316 92-93 0.18891150737542262 2316 94-95 -0.02272011889826686 2316 96-97 0.15007234685484505 2316 98-99 -0.2238848997882137 2316 100-101 -0.5682290389196574 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 3.0 4 7.0 5 16.0 6 46.0 7 125.0 8 282.0 9 591.0 10 971.0 11 1713.0 12 2614.0 13 3678.0 14 4144.0 15 5162.0 16 6143.0 17 7969.0 18 7479.0 19 5936.0 20 5630.0 21 5700.0 22 5959.0 23 6580.0 24 7442.0 25 8840.0 26 10817.0 27 13453.0 28 16780.0 29 20835.0 30 25385.0 31 31257.0 32 37550.0 33 47395.0 34 61286.0 35 81309.0 36 115431.0 37 141621.0 38 112350.0 39 21169.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.70512547916578 18.190256911833497 12.89273369013845 18.211883918862274 2 14.422906483484729 27.567618756237387 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26.578934746854998 25.8361316197358 24.436614031966943 22-23 20.162065758706678 28.889748178292972 26.842649676208417 24.105536386791925 24-25 25.60302691100163 28.157362464458195 26.73235171996824 19.507258904571934 26-27 24.22999567152391 24.495046535671076 32.861329103204106 18.413628689600912 28-29 19.723506053010055 33.638720808743585 28.14429603745029 18.49347710079608 30-31 22.72739966367373 27.603705659873363 26.353652539749028 23.31524213670388 32-33 23.068200098950697 28.73127600870523 28.052826317243223 20.14769757510085 34-35 25.878976916763392 25.48195301762446 29.550415964488824 19.08865410112332 36-37 24.470565377890033 24.763575869438508 32.29636195842238 18.46949679424908 38-39 20.78595547158907 29.214105236829614 31.648391409030257 18.351547882551063 40-41 20.30268775164281 30.672496442395957 27.74512256128064 21.27969324468059 42-43 19.539795891112792 30.901517340514133 25.019546473257755 24.539140295115324 44-45 23.597510192089626 25.497403111410037 26.195977068708885 24.70910962779144 46-47 26.204552145712366 29.7557599910159 26.03130472788197 18.00838313538976 48-49 24.77491204087034 29.072222397587407 27.30484485442127 18.848020707120984 50-51 18.813055587256187 29.884129477781368 32.38692790143721 18.915887033525237 52-53 22.63936878246111 32.70240312355071 24.856254507222484 19.801973586765705 54-55 19.779452650186116 29.055043354320496 26.97747177902044 24.188032216472944 56-57 24.534588877483067 25.75524780748215 27.722094303004845 21.988069012029936 58-59 28.985383200058763 26.023962106436333 25.359015777451194 19.631638916053713 60-61 22.313911613935655 28.29134764586323 31.60558222662558 17.789158513575533 62-63 19.834864780361052 28.35972764831055 30.166136959022786 21.63927061230561 64-65 19.48500272997869 34.42475796112194 26.618233585357842 19.47200572354153 66-67 22.636610653187 26.884041471226677 25.24225450460707 25.237093370979245 68-69 20.70147152646969 29.66278302496696 25.933430743072 23.702314705491347 70-71 25.54561706493136 28.70261722579529 25.25436229592286 20.497403413350494 72-73 23.954081570710883 25.530243614616392 30.92670922375006 19.588965590922665 74-75 20.27898037825461 33.741644186198975 26.709770080055556 19.26960535549086 76-77 22.632630712487163 27.78865041921722 25.13615757614019 24.44256129215543 78-79 23.152190540478195 29.219792200350135 26.39478048460685 21.23323677456482 80-81 25.517435211332582 26.823831642947848 27.35328611423396 20.305447031485606 82-83 23.51956711239101 25.983515402863738 30.31263430612448 20.18428317862077 84-85 20.202069255218667 29.722840721738347 29.29275760673467 20.782332416308314 86-87 20.566135549102068 30.26489913460698 25.684799289037386 23.48416602725357 88-89 20.249660668367977 30.239972416405443 24.17152090987012 25.338846005356462 90-91 23.264916630042322 24.34537525586455 26.144561345574363 26.24514676851877 92-93 25.028227049890855 28.664903032495708 26.106439652388435 20.200430265225002 94-95 23.308819289102832 28.691022882135304 27.247253931301994 20.752903897459866 96-97 17.947910122044867 29.581903001496347 32.02638184472591 20.44380503173288 98-99 21.861115916823735 32.40602302492799 24.485520640788664 21.247340417459608 100-101 18.603247910565905 29.448515664078244 26.18330443819597 25.764931987159873 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 172.0 1 126.5 2 210.0 3 338.5 4 273.0 5 215.5 6 264.0 7 394.0 8 550.0 9 646.5 10 660.5 11 667.0 12 719.5 13 802.0 14 871.0 15 940.5 16 1016.0 17 1105.0 18 1184.5 19 1278.5 20 1426.0 21 1582.5 22 1821.5 23 2167.0 24 2524.0 25 3016.5 26 3745.5 27 4733.0 28 6211.0 29 7773.5 30 9278.0 31 10742.0 32 12136.5 33 13455.5 34 14671.5 35 16096.0 36 17203.0 37 18210.0 38 19413.5 39 20309.5 40 21044.5 41 21636.5 42 22228.0 43 23086.5 44 23733.5 45 24747.0 46 28566.5 47 53235.0 48 109317.0 49 108381.0 50 54922.0 51 30087.5 52 20172.0 53 15523.0 54 13162.5 55 11401.5 56 9696.0 57 7996.0 58 6501.5 59 5282.5 60 4225.0 61 3214.0 62 2342.0 63 1640.5 64 1066.0 65 671.5 66 412.5 67 241.0 68 131.0 69 60.5 70 34.5 71 23.0 72 12.5 73 7.5 74 2.0 75 1.0 76 2.5 77 3.5 78 2.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07685041970284505 2 0.0019425066591556408 3 0.020517726587331458 4 0.0 5 0.0 6 0.0032779799873251443 7 0.0 8 0.0 9 0.0 10-11 9.105499964792067E-4 12-13 0.0 14-15 0.003642199985916827 16-17 0.0 18-19 0.0 20-21 0.013172623282399189 22-23 0.0 24-25 0.0 26-27 0.007587916637326723 28-29 0.009651829962679591 30-31 0.008862686632397612 32-33 0.004309936650001578 34-35 0.006070333309861378 36-37 0.019182253259161952 38-39 0.008377059967608702 40-41 0.01092659995775048 42-43 0.0 44-45 0.0 46-47 6.0703333098613774E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.002974463321832075 58-59 3.035166654930689E-4 60-61 0.007770026636622563 62-63 0.013415436614793646 64-65 0.04995884314015914 66-67 0.026163136565502537 68-69 0.0011533633288736617 70-71 6.0703333098613774E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 7.284399971833653E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 6.0703333098613774E-5 96-97 6.677366640847515E-4 98-99 6.677366640847515E-4 100-101 0.0012140666619722756 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 823678.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.01665076500256 #Duplication Level Percentage of deduplicated Percentage of total 1 86.52664204775348 45.87352763314132 2 8.679435824952265 9.203092359374923 3 2.006202201940286 3.1908636451274184 4 0.818791437239205 1.736383187099418 5 0.4678190756151822 1.240110027654822 6 0.3081754979301492 0.9803059648856095 7 0.2000599491086853 0.7424555917771547 8 0.15932306296678014 0.6757420150496236 9 0.1203716190575338 0.5743530080632075 >10 0.6352923750816969 6.071440077383556 >50 0.04008223092105364 1.427267501276375 >100 0.02573450933749332 2.793552154168462 >500 0.003643824330935589 1.4539462782692931 >1k 0.005921214537770333 6.77844195385454 >5k 0.0013664341241008458 5.350333950253046 >10k+ 0.0011386951034173716 11.908184652621289 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 51470 6.2488011091713025 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 13774 1.6722554202006124 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 12324 1.4962157542146324 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10760 1.3063357282821686 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 10299 1.2503672551652465 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 9725 1.180679828768038 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 8783 1.0663147492102498 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 7598 0.9224478497665349 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6776 0.822651570152414 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 6068 0.7366956504847768 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5363 0.6511039508157314 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4158 0.5048089180480722 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4145 0.5032306313875082 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3684 0.4472621582705863 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 3368 0.4088976517522624 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3148 0.3821881851888724 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2913 0.3536576186325239 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2623 0.31844968543532787 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2442 0.29647507885362967 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2437 0.29586804552264356 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2369 0.2876123922212321 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2359 0.2863983255592598 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2293 0.2783854855902428 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2261 0.27450047227193153 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2047 0.2485194457057248 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1889 0.22933719244656284 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1617 0.19631457924091694 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1616 0.19619317257471972 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1433 0.1739757526606271 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1391 0.16887667268034354 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1250 0.15175833274653444 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1229 0.14920879275639268 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 0.1439883061099119 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1115 0.13536843280990873 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1098 0.13330451948455588 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1050 0.12747699950708893 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1020 0.1238347995211721 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 983 0.1193427528718747 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 951 0.11545773955356342 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 938 0.11387945289299944 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 931 0.11302960622961887 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 922 0.1119369462338438 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 846 0.10271003960285452 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 840 0.10198159960567113 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 833 0.10113175294229056 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.2140666619722756E-4 0.0 12-13 0.0 0.0 0.0 2.4281333239445512E-4 0.0 14-15 0.0 0.0 0.0 4.2492333169029644E-4 0.0 16-17 0.0 0.0 0.0 7.284399971833654E-4 0.0 18-19 0.0 0.0 0.0 9.105499964792067E-4 0.0 20-21 0.0 0.0 0.0 0.001578286660563958 0.0 22-23 0.0 0.0 0.0 0.0031565733211279166 0.0 24-25 0.0 0.0 0.0 0.0052204866464807854 0.0 26-27 0.0 0.0 0.0 0.00770932330352395 0.0 28-29 0.0 0.0 0.0 0.015661459939442356 0.0 30-31 0.0 0.0 0.0 0.031201513212687486 0.0 32-33 0.0 0.0 0.0 0.05748605644438725 0.0 34-35 0.0 0.0 0.0 0.0780037830317187 0.0 36-37 0.0 0.0 0.0 0.09700392629158483 0.0 38-39 0.0 6.070333309861378E-5 0.0 0.12152807286342479 0.0 40-41 0.0 1.2140666619722756E-4 0.0 0.14641643943385643 0.0 42-43 0.0 1.2140666619722756E-4 0.0 0.21677160249514982 0.0 44-45 0.0 1.2140666619722756E-4 0.0 0.2867625455578515 0.0 46-47 0.0 1.2140666619722756E-4 0.0 0.33726771869589817 0.0 48-49 0.0 1.2140666619722756E-4 0.0 0.38929047516141013 0.0 50-51 0.0 1.2140666619722756E-4 0.0 0.4567318782339701 0.0 52-53 0.0 1.2140666619722756E-4 0.0 0.6154103909537465 0.0 54-55 0.0 1.2140666619722756E-4 0.0 0.7036730372791309 0.0 56-57 0.0 1.2140666619722756E-4 0.0 0.9719210662419052 0.0 58-59 0.0 1.2140666619722756E-4 0.0 1.247635605175809 0.0 60-61 0.0 1.2140666619722756E-4 0.0 1.4242216011596764 0.0 62-63 0.0 1.2140666619722756E-4 0.0 1.7426712865950043 0.0 64-65 0.0 1.2140666619722756E-4 0.0 1.872576419426038 0.0 66-67 0.0 1.2140666619722756E-4 0.0 2.396871107398765 0.0 68-69 0.0 1.2140666619722756E-4 0.0 2.8898428754926075 0.0 70-71 0.0 1.2140666619722756E-4 0.0 3.061876121494079 0.0 72-73 0.0 1.2140666619722756E-4 0.0 3.4594222499569005 0.0 74-75 0.0 1.2140666619722756E-4 0.0 3.786006182027443 0.0 76-77 0.0 1.2140666619722756E-4 0.0 3.9023137682443867 0.0 78-79 0.0 1.2140666619722756E-4 0.0 4.071251144257829 0.0 80-81 0.0 1.2140666619722756E-4 0.0 4.431525426198101 0.0 82-83 0.0 1.2140666619722756E-4 0.0 4.664626225296779 0.0 84-85 0.0 1.2140666619722756E-4 0.0 5.239001163075862 0.0 86-87 0.0 1.2140666619722756E-4 0.0 6.495802971549562 0.0 88-89 0.0 1.2140666619722756E-4 0.0 8.182566488360743 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCG 75 0.0 63.402428 1 TACCTGG 1170 0.0 58.47555 2 TATAACG 165 0.0 54.710083 2 ACCTGGG 1315 0.0 49.495552 3 ATAACGC 210 0.0 47.508503 3 GTACCTG 1560 0.0 44.198807 1 TAACGCA 265 0.0 39.42426 4 GGCGCGT 25 0.0016179737 37.990654 44-45 GTATAAC 295 0.0 35.462376 1 CCTGGGG 785 0.0 33.272068 4 TACCCGG 145 6.6211214E-10 32.766476 2 AGTACGC 825 0.0 32.250153 2 GTACACG 105 1.8846986E-6 31.701214 1 TACGCAG 855 0.0 31.658876 4 GTACGCA 865 0.0 31.306181 3 GAGTACG 885 0.0 31.163904 1 GTACCGG 65 0.0058120685 29.262661 1 TAGCCCG 65 0.0058501516 29.223577 5 CATAGCG 65 0.0058501516 29.223577 7 ACGCAGT 960 0.0 27.701517 5 >>END_MODULE