##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003843_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1212204 Sequences flagged as poor quality 0 Sequence length 121 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.155733688389084 31.0 31.0 34.0 28.0 34.0 2 30.921158484875484 33.0 31.0 34.0 27.0 34.0 3 31.12987005487525 33.0 31.0 34.0 27.0 34.0 4 34.487676991661466 37.0 35.0 37.0 32.0 37.0 5 34.40941458698371 37.0 35.0 37.0 32.0 37.0 6 34.52576051555679 37.0 35.0 37.0 32.0 37.0 7 34.49766375956522 37.0 35.0 37.0 32.0 37.0 8 34.59030658206044 37.0 35.0 37.0 32.0 37.0 9 36.09682693672022 39.0 37.0 39.0 32.0 39.0 10-11 36.00865200906778 39.0 37.0 39.0 32.0 39.0 12-13 36.0098073426585 39.0 37.0 39.0 32.0 39.0 14-15 37.017618321668635 40.0 37.0 41.0 31.5 41.0 16-17 37.00739190763271 40.0 37.0 41.0 31.0 41.0 18-19 36.955621331063085 40.0 37.0 41.0 31.0 41.0 20-21 36.89625384836216 40.0 37.0 41.0 30.5 41.0 22-23 36.71615049942089 40.0 37.0 41.0 30.5 41.0 24-25 36.57057516721608 39.5 36.5 41.0 30.0 41.0 26-27 36.441499945553716 39.0 36.0 41.0 30.0 41.0 28-29 36.3332566135733 39.0 36.0 41.0 30.0 41.0 30-31 36.05346624825525 39.0 36.0 40.5 29.0 41.0 32-33 35.80608709425147 39.0 35.0 40.0 27.5 41.0 34-35 35.60622180755054 39.0 35.0 40.0 26.5 41.0 36-37 35.56865923557421 38.5 35.0 40.0 27.0 41.0 38-39 35.47863560918789 38.5 35.0 40.0 27.0 41.0 40-41 35.752240959442474 39.0 35.0 41.0 27.0 41.0 42-43 35.60078336649607 39.0 35.0 41.0 27.0 41.0 44-45 35.342999198154764 39.0 35.0 41.0 25.5 41.0 46-47 34.98518112462919 39.0 35.0 40.0 24.0 41.0 48-49 34.65276925335999 38.5 34.0 40.0 23.0 41.0 50-51 33.81893270439629 37.5 33.5 39.5 22.0 40.5 52-53 33.97503802990256 38.0 33.5 40.0 22.5 40.5 54-55 34.340716991529476 38.0 34.0 40.0 22.5 41.0 56-57 33.807550544297825 38.0 33.5 40.0 17.5 41.0 58-59 33.454471772077966 38.0 32.5 40.0 15.0 41.0 60-61 32.78094281160597 37.5 31.0 40.0 12.0 41.0 62-63 32.77112845692639 37.0 31.5 40.0 12.0 41.0 64-65 32.465727715797016 36.5 31.5 39.5 8.0 41.0 66-67 31.90740626165233 36.0 31.0 39.0 7.5 40.5 68-69 31.43844311683512 36.0 30.5 39.0 4.5 40.0 70-71 30.668538876294747 35.0 29.5 37.5 2.0 40.0 72-73 30.238897908272865 35.0 29.0 37.0 2.0 39.0 74-75 29.858372435662645 34.5 29.0 37.0 2.0 39.0 76-77 29.465668732325582 34.0 29.0 36.0 2.0 38.5 78-79 28.73053875420309 34.0 28.0 36.0 2.0 37.5 80-81 28.12232512019429 34.0 26.0 35.0 2.0 37.0 82-83 27.579946939624023 33.5 25.5 35.0 2.0 36.5 84-85 27.285658189545654 33.0 25.5 35.0 2.0 36.0 86-87 27.385745303595762 33.5 26.0 35.0 2.0 36.0 88-89 26.969590101996033 33.0 25.0 35.0 2.0 35.5 90-91 26.586701578282202 33.0 25.0 35.0 2.0 35.0 92-93 26.18361142183989 32.5 23.5 35.0 2.0 35.0 94-95 25.89609710906745 32.0 21.5 35.0 2.0 35.0 96-97 25.664737948398123 32.0 20.0 35.0 2.0 35.0 98-99 25.38092763264269 32.0 19.0 35.0 2.0 35.0 100-101 25.050440767395585 32.0 18.5 35.0 2.0 35.0 102-103 24.411177491577327 31.5 13.5 34.0 2.0 35.0 104-105 23.806595259543773 31.0 7.5 34.0 2.0 35.0 106-107 23.116073697166485 30.5 4.5 34.0 2.0 35.0 108-109 22.95122933103669 30.5 2.0 34.0 2.0 35.0 110-111 22.63443859284411 30.5 2.0 34.0 2.0 35.0 112-113 21.853106820304173 29.5 2.0 34.0 2.0 35.0 114-115 21.162642179039175 29.0 2.0 34.0 2.0 35.0 116-117 20.23895565432881 29.0 2.0 34.0 2.0 35.0 118-119 19.383781525221828 27.0 2.0 34.0 2.0 35.0 120-121 17.77464312937426 22.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8146119889464956 1101 2 0.8695273794173168 1101 3 1.015567016130852 1101 4 1.260579211634571 1101 5 1.383478821091714 1101 6 1.263417213793275 1101 7 1.1319091720698253 1101 8 1.1823400553356933 1101 9 1.2755285859499779 1101 10-11 1.2657425899672674 1101 12-13 0.9677375259961067 1101 14-15 1.2263567136548161 1101 16-17 1.2732575977188958 1101 18-19 1.401111220986337 1101 20-21 1.3341493287442603 1101 22-23 1.5286378796240285 1101 24-25 1.3156590886095216 1101 26-27 1.1772207705642188 1101 28-29 1.292196003567831 1101 30-31 1.2695657146856973 1101 32-33 1.2110402405276588 1101 34-35 1.2318375424313217 1101 36-37 1.1511662097715671 1101 38-39 1.0186426604704337 1101 40-41 1.2718099778181653 1101 42-43 1.0933918744489048 1101 44-45 0.9423570732884841 1101 46-47 1.189932073097367 1101 48-49 1.2657447960079509 1101 50-51 1.0915088130908686 1101 52-53 1.2331404563370896 1101 54-55 1.1757016647448708 1101 56-57 1.0402080731079408 1101 58-59 1.0224213729738238 1101 60-61 1.1523425154904388 1101 62-63 0.9778556134820704 1101 64-65 0.925912865779452 1101 66-67 0.9108587888870971 1101 68-69 0.739423263830826 1101 70-71 0.32108384536608625 1101 72-73 0.009916675536413067 1101 74-75 0.3921137995577375 1101 76-77 0.3508386901618472 1101 78-79 0.34058996887343085 1101 80-81 0.4139017875652904 1101 82-83 0.26144638531503617 1101 84-85 0.3914676143553564 1101 86-87 0.6166620954789366 1101 88-89 0.43458148118877915 1101 90-91 0.34434011040207224 1101 92-93 0.18625470159059887 1101 94-95 0.3333495300426499 1101 96-97 0.24341589778338601 1101 98-99 0.25366131651328416 1101 100-101 0.36248956663557763 1101 102-103 0.1703639577531213 1101 104-105 -0.06148063458976338 1101 106-107 -0.058955305491259935 1101 108-109 -0.2828635141663831 1101 110-111 -0.27357311830518327 1101 112-113 -0.3782625616620656 1101 114-115 -0.611181099571219 1101 116-117 -0.7147670687797039 1101 118-119 -0.7994194514737352 1101 120-121 -0.6108988687902048 1102 1 0.6623478350250664 1102 2 0.8578931304612247 1102 3 0.8276512723649248 1102 4 1.0780807417645377 1102 5 1.0099324445622386 1102 6 1.067153712259639 1102 7 0.9633721516932283 1102 8 0.9018290883812341 1102 9 1.070780535659999 1102 10-11 1.0363291953235105 1102 12-13 0.8615526442063199 1102 14-15 1.0984001430399744 1102 16-17 0.9324218047783575 1102 18-19 1.1125392312211062 1102 20-21 1.0878746910211632 1102 22-23 0.9328219301455007 1102 24-25 0.9115504485048618 1102 26-27 0.923145539027928 1102 28-29 0.9930016674162587 1102 30-31 1.110924996217335 1102 32-33 1.0061711828246658 1102 34-35 1.0269211340014905 1102 36-37 0.831503206648172 1102 38-39 0.888168609545609 1102 40-41 0.7472744095305188 1102 42-43 1.1060494252237802 1102 44-45 1.0004292322887167 1102 46-47 1.0225647910466265 1102 48-49 1.022240175804626 1102 50-51 0.5976363478892637 1102 52-53 0.8220142631426199 1102 54-55 0.8899794872427833 1102 56-57 0.963400809857184 1102 58-59 0.9035780635438471 1102 60-61 0.9376253834713495 1102 62-63 0.6024406369732915 1102 64-65 0.5998838204928632 1102 66-67 0.7367115842460237 1102 68-69 0.6089176457547758 1102 70-71 0.5085558952842746 1102 72-73 0.603583428135881 1102 74-75 0.611592310219546 1102 76-77 0.6625494017731235 1102 78-79 0.6046149911019647 1102 80-81 0.7251521448879039 1102 82-83 0.7002622313234568 1102 84-85 0.5257858428068296 1102 86-87 0.6487106457575145 1102 88-89 0.7025673040304206 1102 90-91 0.5120546311162606 1102 92-93 0.4882251933191242 1102 94-95 0.48401603536609983 1102 96-97 0.24877880602995361 1102 98-99 0.2793061194202693 1102 100-101 0.4291532519601837 1102 102-103 0.2790987638694311 1102 104-105 0.030838376025570824 1102 106-107 0.009021924070324872 1102 108-109 0.06191466521570632 1102 110-111 0.06454351530366154 1102 112-113 0.10811972004669812 1102 114-115 -0.15891238595016688 1102 116-117 -0.4354582841676411 1102 118-119 -0.42448587402109794 1102 120-121 -0.29545008035500686 1103 1 0.5489484353894802 1103 2 0.8715284811762878 1103 3 0.815865515945088 1103 4 0.9488621330894063 1103 5 1.1909547681560326 1103 6 1.0904532259382478 1103 7 1.0048942983237197 1103 8 0.9003288327675634 1103 9 0.8705085364989387 1103 10-11 0.9849570742543889 1103 12-13 0.9190234452602297 1103 14-15 1.1482058111092428 1103 16-17 1.089127731082968 1103 18-19 1.0649764092461922 1103 20-21 1.2364393219543572 1103 22-23 1.3465821383067862 1103 24-25 1.2963007991543805 1103 26-27 1.385066639608631 1103 28-29 1.3746187843562296 1103 30-31 1.2581106241749183 1103 32-33 1.2211226162274684 1103 34-35 1.2837912429322387 1103 36-37 1.0962970116214947 1103 38-39 0.9074697355565959 1103 40-41 1.166157377795102 1103 42-43 1.1875407015880413 1103 44-45 1.097074139022503 1103 46-47 1.0372189916312564 1103 48-49 0.9925735877883142 1103 50-51 0.9615928542402798 1103 52-53 0.9539505694595576 1103 54-55 1.2158924868364238 1103 56-57 1.277013548593807 1103 58-59 1.1075623596886643 1103 60-61 1.256368419637404 1103 62-63 1.1864896885810268 1103 64-65 1.363357937970278 1103 66-67 1.3438631959229852 1103 68-69 1.344441391609248 1103 70-71 1.0728181549091111 1103 72-73 0.7076254012112386 1103 74-75 1.002787730146924 1103 76-77 1.1668132344051578 1103 78-79 1.025659533422413 1103 80-81 1.1306498050397025 1103 82-83 1.1546981116176092 1103 84-85 1.0814480132945938 1103 86-87 0.9374882225399297 1103 88-89 1.0209744376180545 1103 90-91 1.0188103693154282 1103 92-93 1.090535547639 1103 94-95 0.9130694782856033 1103 96-97 0.6207491286275122 1103 98-99 0.7675793507703013 1103 100-101 0.7786915088060411 1103 102-103 0.7745717802418142 1103 104-105 0.7769428892453831 1103 106-107 0.38255378402025286 1103 108-109 0.3458975194372691 1103 110-111 0.4418768093118146 1103 112-113 0.5339435825658718 1103 114-115 0.004653162570548375 1103 116-117 -0.08178924488178296 1103 118-119 -0.20439562273319822 1103 120-121 -0.007024136222977262 1104 1 0.6311763769394538 1104 2 0.7760917145530826 1104 3 0.6984185561252474 1104 4 1.2194034561730973 1104 5 1.235875102059417 1104 6 1.2071560146749363 1104 7 1.0281557826021483 1104 8 1.0678672265771496 1104 9 1.0751945284730624 1104 10-11 1.1626340582873098 1104 12-13 1.0410425278725413 1104 14-15 1.2723371882067127 1104 16-17 1.2716718818474675 1104 18-19 1.14184403314772 1104 20-21 1.0560121220356322 1104 22-23 1.1582886521319438 1104 24-25 1.1612130055920105 1104 26-27 1.0855056074204867 1104 28-29 1.1899860442150114 1104 30-31 1.2317226054140207 1104 32-33 1.193777080407358 1104 34-35 1.0358959179182676 1104 36-37 1.1458910616392686 1104 38-39 1.1547948680512192 1104 40-41 1.2195527112751776 1104 42-43 1.2505653887652741 1104 44-45 0.9568795878110024 1104 46-47 1.2382937643162037 1104 48-49 1.382522198499636 1104 50-51 1.0656405015082697 1104 52-53 1.1778042581437518 1104 54-55 1.4184826932401862 1104 56-57 1.3758591944733425 1104 58-59 1.264414829252999 1104 60-61 1.4437970926592953 1104 62-63 1.4515644443337479 1104 64-65 1.5829595228261013 1104 66-67 1.3837694760835006 1104 68-69 1.3183945570878386 1104 70-71 0.7593202121738791 1104 72-73 0.8030494686692045 1104 74-75 0.916922324366265 1104 76-77 0.7924473460562176 1104 78-79 1.1151408148528468 1104 80-81 0.8586184465676645 1104 82-83 0.7671813910500411 1104 84-85 0.8507702165151052 1104 86-87 0.8179370110395645 1104 88-89 0.8120981444197248 1104 90-91 0.8382218899981346 1104 92-93 1.1184627052271736 1104 94-95 1.013682424661262 1104 96-97 0.5917950318768064 1104 98-99 0.6782737411257074 1104 100-101 0.9051188949791928 1104 102-103 0.5382394684857736 1104 104-105 0.3262533245727681 1104 106-107 0.1389816781600075 1104 108-109 0.2045829018514631 1104 110-111 0.3926442621979085 1104 112-113 0.20832809635716387 1104 114-115 0.15476195208611898 1104 116-117 -0.029848812432877736 1104 118-119 -0.11450119512690549 1104 120-121 -0.1893257329313549 1105 1 0.6487817224136521 1105 2 1.0783795906114477 1105 3 0.9543920907873087 1105 4 1.1109627165848224 1105 5 1.2645922865143078 1105 6 1.2318198754043692 1105 7 1.2597111125181328 1105 8 1.0875852135082482 1105 9 1.2043850101225644 1105 10-11 1.1765668468102675 1105 12-13 1.0225651036574064 1105 14-15 1.1819562576618523 1105 16-17 1.280376292191221 1105 18-19 1.172677953325902 1105 20-21 1.0108032325644416 1105 22-23 1.1558853177613955 1105 24-25 1.2176360907036212 1105 26-27 1.306903062424496 1105 28-29 1.3444586208187346 1105 30-31 1.293124318455071 1105 32-33 1.2494836689008295 1105 34-35 1.5848510611357085 1105 36-37 1.170288795053402 1105 38-39 1.3170547393274816 1105 40-41 1.2884264604767992 1105 42-43 1.394650811539222 1105 44-45 1.2969411655775005 1105 46-47 1.2461629443165307 1105 48-49 1.3707470883638706 1105 50-51 1.2259678790487598 1105 52-53 1.5368714204896037 1105 54-55 1.3894297789413699 1105 56-57 1.6353005727671857 1105 58-59 1.4125617642851793 1105 60-61 1.2564557454818868 1105 62-63 0.8836119050697349 1105 64-65 1.3251886052191253 1105 66-67 1.217579109073462 1105 68-69 1.1400155590367085 1105 70-71 0.7658628498640603 1105 72-73 1.2029733153143631 1105 74-75 1.1723636043977095 1105 76-77 1.079885317920418 1105 78-79 0.9771926973965321 1105 80-81 1.2494407390292999 1105 82-83 1.0170626573247503 1105 84-85 0.6324066129055943 1105 86-87 0.7272899531793939 1105 88-89 0.9125345932168294 1105 90-91 0.6479285767540013 1105 92-93 0.4574311138677132 1105 94-95 0.6012546884630332 1105 96-97 0.5098155359628826 1105 98-99 0.359416368989649 1105 100-101 0.38916763312629143 1105 102-103 0.14560341601365323 1105 104-105 -0.2123269476140628 1105 106-107 -0.22761074580946428 1105 108-109 -0.5802347395235579 1105 110-111 -0.6307872243483281 1105 112-113 -0.740206975128725 1105 114-115 -0.6918627347025037 1105 116-117 -0.7390412593302393 1105 118-119 -0.3851690518603341 1105 120-121 -0.5595129227906774 1106 1 0.6180491532859129 1106 2 1.1799860658158536 1106 3 0.9152368255750964 1106 4 1.0876667718048338 1106 5 0.9349567221936752 1106 6 1.034153578514605 1106 7 1.158762018471542 1106 8 1.071109598240568 1106 9 1.162826195052098 1106 10-11 1.1124824555041286 1106 12-13 0.8214989135920874 1106 14-15 1.0921621001368962 1106 16-17 1.1670396719520966 1106 18-19 1.1111203708451072 1106 20-21 1.053974685787665 1106 22-23 1.2757238485145095 1106 24-25 1.1609369658422892 1106 26-27 1.2529259064197333 1106 28-29 1.1408553503474792 1106 30-31 1.261241332301168 1106 32-33 1.2133277052211398 1106 34-35 1.4928074274613579 1106 36-37 1.2737544263447447 1106 38-39 1.0909464357817313 1106 40-41 1.220521041190878 1106 42-43 1.2608840175049565 1106 44-45 1.3825547898019934 1106 46-47 1.365867944521959 1106 48-49 1.5130495658691103 1106 50-51 1.6556075520016336 1106 52-53 1.4850102100865499 1106 54-55 1.339951575235382 1106 56-57 1.4866913447791816 1106 58-59 1.7020868063986612 1106 60-61 1.8825907551371657 1106 62-63 1.3995944844690555 1106 64-65 1.455083013370647 1106 66-67 1.5480569430025497 1106 68-69 1.6592028715803586 1106 70-71 1.200186363566349 1106 72-73 1.3431803903790716 1106 74-75 1.660910792038944 1106 76-77 1.4385380974101274 1106 78-79 1.1726403328786787 1106 80-81 1.0402394571360425 1106 82-83 1.0747992118257557 1106 84-85 1.1336802888988586 1106 86-87 1.2776001549132339 1106 88-89 1.3479263513005684 1106 90-91 1.5404056711292888 1106 92-93 1.0428803928027577 1106 94-95 1.1907509701508587 1106 96-97 1.121673048596925 1106 98-99 0.9187963763851883 1106 100-101 0.820738363230241 1106 102-103 0.5342697539897436 1106 104-105 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1.0549343439328922 2210 54-55 1.0317686856602748 2210 56-57 1.0054517906925184 2210 58-59 0.9507816794803432 2210 60-61 1.2780980893174814 2210 62-63 1.2996622274886604 2210 64-65 1.2019980309205351 2210 66-67 1.391087173419237 2210 68-69 1.2833775596535197 2210 70-71 1.1558911697791139 2210 72-73 1.2795543228048487 2210 74-75 1.3918704069511456 2210 76-77 1.3192840090925202 2210 78-79 1.0854149784523273 2210 80-81 1.2072094226390995 2210 82-83 1.1134764069181173 2210 84-85 1.248673876294891 2210 86-87 1.20346939807526 2210 88-89 1.3014500432725278 2210 90-91 1.2696626681015708 2210 92-93 1.2106630484798302 2210 94-95 1.1362958304977582 2210 96-97 0.9899053400893365 2210 98-99 1.176311782458999 2210 100-101 1.1566028289646297 2210 102-103 1.051662906142525 2210 104-105 1.253707109513023 2210 106-107 1.5237436389893162 2210 108-109 1.5330037293703036 2210 110-111 1.3616677222015277 2210 112-113 1.5894989399453259 2210 114-115 1.518476709968045 2210 116-117 1.208269052864786 2210 118-119 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1.0168597507281376 2211 58-59 0.9091003383452829 2211 60-61 1.0388229080240947 2211 62-63 1.0573936433832856 2211 64-65 1.0414182016237703 2211 66-67 1.0979592411207193 2211 68-69 1.2558663759155841 2211 70-71 1.1763178410359174 2211 72-73 0.9091633798678629 2211 74-75 0.8923828337250761 2211 76-77 1.0367284886424422 2211 78-79 1.0750788680112393 2211 80-81 1.1753453480403309 2211 82-83 1.3402285573175483 2211 84-85 1.2015358977892596 2211 86-87 1.0645911729479671 2211 88-89 1.0886611921692158 2211 90-91 1.0284619700687223 2211 92-93 1.0562919820395003 2211 94-95 0.9166476327112143 2211 96-97 1.0513831524818755 2211 98-99 1.1705297588054577 2211 100-101 1.417564704035172 2211 102-103 1.3251995266322716 2211 104-105 1.2066823744933401 2211 106-107 1.3493616936247719 2211 108-109 1.5042151591108386 2211 110-111 1.6023444803727038 2211 112-113 1.53414760014277 2211 114-115 1.4217435580507782 2211 116-117 1.4626865868821852 2211 118-119 1.6085516465700032 2211 120-121 1.5009168556163175 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-0.47375491107234424 2316 104-105 -0.2992118617989341 2316 106-107 0.044819112680162476 2316 108-109 -0.08003156300379644 2316 110-111 -0.05161721287093002 2316 112-113 -0.3960367314495912 2316 114-115 -0.22249429025510992 2316 116-117 0.02018295466688258 2316 118-119 -2.7476999396114365E-4 2316 120-121 -0.3249899978449129 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20483.0 3 3386.0 4 2749.0 5 3220.0 6 3531.0 7 3728.0 8 4336.0 9 4923.0 10 5677.0 11 6505.0 12 7221.0 13 8604.0 14 10696.0 15 11554.0 16 9757.0 17 10104.0 18 10876.0 19 11826.0 20 12739.0 21 14290.0 22 15979.0 23 17595.0 24 19672.0 25 21895.0 26 24889.0 27 28824.0 28 33209.0 29 39303.0 30 45901.0 31 55100.0 32 66767.0 33 82423.0 34 103177.0 35 127556.0 36 158109.0 37 154857.0 38 50515.0 39 228.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.34371071679039 16.937844865229646 14.733436544797746 20.98500787318222 2 13.276604692378683 21.000555715947378 34.998172645758615 30.72466694591532 3 27.355770353248815 21.21177251456722 31.599192173892742 19.833264958291224 4 10.05203973761701 13.419782770361527 46.138037863452794 30.39013962856866 5 9.703367856207864 38.468881889081025 38.60570052643076 13.222049728280355 6 26.286997370224675 34.21109241567459 18.214821126917926 21.287089087182807 7 20.89042608973285 33.73905765586875 24.708336146811018 20.662180107587385 8 19.997682481645338 24.671983620647257 22.971525368906406 32.358808528801006 9 17.40938680885012 21.516001057039272 31.360721320932296 29.713890813178313 10-11 24.684611316190573 20.15128320651379 28.014567333280265 27.149538144015377 12-13 29.917332294169498 20.860144246498553 26.62925399011822 22.593269469213727 14-15 24.195330463931942 22.059450540336318 33.76959237523335 19.975626620498396 16-17 18.848984199210168 27.551381954269168 31.982114169522724 21.617519676997944 18-19 16.510732893597453 33.103139757546295 29.610045356361585 20.776081992494667 20-21 19.887930074903664 26.096800700051404 24.738053955486368 29.27721526955856 22-23 17.108745410080086 25.60947091859257 29.65076800526218 27.631015666065167 24-25 24.950346066553987 23.775991463483255 26.61021505936589 24.663447410596866 26-27 21.866286497966804 20.623109838324087 35.74244404287736 21.768159620831746 28-29 17.877241477071372 30.258283198459058 30.619421062067183 21.245054262402387 30-31 18.090224096783395 25.43553527560552 28.94495894811101 27.529281679500073 32-33 20.37816369805273 24.40632585349909 31.47146303289754 23.74404741555064 34-35 25.515783504767782 22.95606636118396 27.830002626762116 23.698147507286137 36-37 21.634749092382854 21.282876755452687 35.561663552672236 21.520710599492226 38-39 21.88067693095393 25.581540793849932 31.420818683020812 21.116963592175324 40-41 19.34896772566082 29.23028171509937 31.371174705890937 20.049575853348873 42-43 17.99087348160625 28.418327249682612 25.240139170024662 28.350660098686475 44-45 19.161934015101963 24.55917867599148 26.413552663229055 29.8653346456775 46-47 24.384289335964258 26.632553047525466 28.928676993860297 20.05448062264998 48-49 22.748127182730016 24.294741353397878 29.26168329887658 23.695448164995526 50-51 15.238246934871333 26.62158691188808 36.558132785827105 21.582033367413484 52-53 19.344441767021394 29.935739067428536 26.612297376282907 24.107521789267164 54-55 16.71254282910136 24.307167104581808 31.305206701227355 27.675083365089474 56-57 22.950922890503232 21.396523930605372 28.02162932100644 27.630923857884955 58-59 26.88649767727481 22.97828273837689 27.064344677993123 23.070874906355172 60-61 23.288300236217317 23.190918978793825 34.57026297527058 18.950517809718274 62-63 19.317565843350284 26.55741560159248 31.981746827293694 22.143271727763537 64-65 16.987197662355857 32.17396235352198 29.050181961312788 21.788658022809376 66-67 19.5207935488713 25.977843008080587 24.744781807416796 29.756581635631314 68-69 17.284349563918642 26.13640296361034 29.246742784757977 27.332504687713044 70-71 24.384960215454775 24.00217423770275 26.033659802716713 25.579205744125762 72-73 21.283038200282185 20.91975045452687 34.70611990498115 23.091091440209794 74-75 17.948142421641855 29.746536271058876 30.09077099650342 22.214550310795854 76-77 18.019657307276024 25.040994448120845 28.71822773803275 28.22112050657038 78-79 20.317477802716123 24.199264200566056 31.088464923096566 24.39479307362125 80-81 24.703463433655926 23.46772932293198 27.319566346159863 24.509240897252234 82-83 20.665385874995568 21.917891658374895 34.66448055971013 22.752241906919405 84-85 21.132374666024923 25.33305750585786 30.495605250843123 23.0389625772741 86-87 19.377947032543872 28.255773754640916 30.597970027060466 21.76830918575474 88-89 18.38774586771259 26.058714241925696 25.942945554102888 29.610594336258828 90-91 18.53049294699675 22.11755255657292 28.1195494686162 31.23240502781413 92-93 22.84784893322435 24.07589203796687 30.79478539376449 22.28147363504429 94-95 20.858096240486123 22.251463256377196 31.271611074878358 25.618829428258323 96-97 13.641565317917145 25.332405270440805 37.2047846674446 23.821244744197454 98-99 17.723731407597683 28.907976238099547 27.63945683509206 25.72883551921071 100-101 15.005370188467545 24.241635540428653 31.664925265746124 29.088069005357674 102-103 20.206979263708615 22.008049981276205 29.08531128623722 28.699659468777966 104-105 24.27095246811479 23.184297328726313 27.752318346342868 24.792431856816027 106-107 21.671203243652545 22.98276601103122 33.50779140418732 21.838239341128908 108-109 19.13866919733546 24.9569280393877 31.417221779318123 24.487180983958716 110-111 17.794476496421467 28.829849869985175 28.507064500364443 24.868609133228922 112-113 20.539758642272087 23.55095503554149 24.264245055527457 31.645041266658968 114-115 18.329788919746136 23.611298756536318 28.30781355550552 29.75109876821203 116-117 24.576661425073723 22.164221748578722 25.12077214086399 28.138344685483567 118-119 21.31655480097945 20.425230025687473 32.678933313091434 25.579281860241643 120-121 17.76692166711495 28.206135440058215 29.30215780837227 24.724785084454563 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3199.0 1 3959.5 2 2705.0 3 633.0 4 608.0 5 653.0 6 651.5 7 590.0 8 560.0 9 531.5 10 502.5 11 523.0 12 556.0 13 605.5 14 657.5 15 708.5 16 783.5 17 881.0 18 966.0 19 1065.0 20 1228.0 21 1412.0 22 1571.0 23 1890.0 24 2356.0 25 2994.0 26 3820.5 27 4833.5 28 6537.5 29 8648.0 30 10843.5 31 13025.0 32 15082.0 33 16952.0 34 18556.0 35 20187.5 36 21672.5 37 23361.5 38 24884.5 39 25559.0 40 26118.5 41 26557.5 42 27227.0 43 28648.0 44 29742.0 45 32449.0 46 43880.5 47 94604.5 48 196966.0 49 194818.5 50 93772.5 51 43219.5 52 26595.0 53 20521.5 54 17096.0 55 14311.5 56 11999.0 57 9538.5 58 7327.0 59 5696.5 60 4409.0 61 3313.0 62 2440.0 63 1739.0 64 1234.0 65 892.5 66 595.0 67 376.0 68 259.5 69 188.0 70 126.0 71 80.0 72 64.5 73 52.0 74 40.5 75 24.0 76 17.0 77 12.5 78 13.5 79 13.0 80 7.5 81 5.5 82 4.5 83 5.5 84 4.0 85 4.0 86 4.0 87 2.5 88 3.0 89 3.5 90 4.0 91 3.0 92 3.0 93 3.0 94 2.0 95 2.0 96 2.0 97 1.5 98 1.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2504528940673352 2 1.1342975274788731 3 1.067147113852124 4 1.0506482407251585 5 1.0564228463195964 6 1.0610425307951465 7 1.0411613886771534 8 1.0431412534523892 9 1.0416563548709623 10-11 1.033943131684106 12-13 1.032953199296488 14-15 1.0340256260497407 16-17 1.0482559041217485 18-19 1.0661159342816886 20-21 1.0611662723435988 22-23 1.047719690745122 24-25 1.0436774668290156 26-27 1.050689487907976 28-29 1.0673945969490284 30-31 1.0716018095964046 32-33 1.067064619486489 34-35 1.0731692025434663 36-37 1.076345235620407 38-39 1.0722205173386659 40-41 1.07502532577025 42-43 1.0684257765194638 44-45 1.057866497718206 46-47 1.0624036878281213 48-49 1.062073710365582 50-51 1.0782013588471906 52-53 1.0621149575483995 54-55 1.0692094729929946 56-57 1.079933740525522 58-59 1.060753800515425 60-61 1.0978350178682796 62-63 1.0782013588471906 64-65 1.0763864828032246 66-67 1.075231561684337 68-69 1.0772526736423902 70-71 1.1238207430432503 72-73 1.1305852810253059 74-75 1.1341737859304208 76-77 1.1442793457206872 78-79 1.1479503449914372 80-81 1.0989486918043496 82-83 1.1323589098864546 84-85 1.104352072753431 86-87 1.090781749606502 88-89 1.09399902986626 90-91 1.101547264321847 92-93 1.0942052657803472 94-95 1.086120817948134 96-97 1.0964326136524876 98-99 1.1188298339223433 100-101 1.1100854311650514 102-103 1.088513154551544 104-105 1.0740353933826319 106-107 1.077541403922112 108-109 1.0763864828032246 110-111 1.081501133472584 112-113 1.0817073693866708 114-115 1.0834809982478197 116-117 1.1102916670791385 118-119 1.1029909157204563 120-121 1.087811952443648 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 1212204.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.92467900887672 #Duplication Level Percentage of deduplicated Percentage of total 1 90.48156658447336 43.36300034781192 2 5.7627193412622155 5.523529492964744 3 1.3830166605788845 1.9884188856651492 4 0.6161834451591828 1.1812157527935054 5 0.36190178439778853 0.8672013425001863 6 0.24425701302132263 0.7023563366828346 7 0.17577227613186744 0.5896680938595573 8 0.1285116472977589 0.492710355651766 9 0.10366600314048743 0.44713439321769605 >10 0.6456078276126492 5.796013741646986 >50 0.04985135472539734 1.6547660131182589 >100 0.034654729619271 3.219202904167926 >500 0.004097110859958682 1.4110397544177038 >1k 0.004950675622450073 4.5748548146071295 >5k 0.0015364165724845054 5.73063602934934 >10k+ 0.0017071295249827837 22.4582517415453 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 63633 5.249363968440955 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 41440 3.418566511907237 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 41020 3.3839188783406096 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 40294 3.324027968889725 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 25960 2.1415537318801126 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 18435 1.5207836304780384 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 12190 1.0056063170885428 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 10977 0.9055406515734976 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 10467 0.8634685250997356 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 10089 0.832285654889771 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 9514 0.7848513946497455 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 8923 0.7360972245595626 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 8612 0.7104414768471313 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 8364 0.6899828741696942 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8350 0.6888279530508066 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 7887 0.6506330617618816 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 6495 0.5358009047982023 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 6456 0.5325836245384441 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 5444 0.449099326515999 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 4140 0.3415266737281844 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 3931 0.3242853513105055 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3799 0.3133960950467083 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 3601 0.2970622106510125 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 3529 0.291122616325305 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 3202 0.26414695876271654 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3173 0.2617546221593065 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2567 0.2117630365846013 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1757 0.14494260042039128 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1743 0.1437876793015037 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1579 0.13025860333739206 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1533 0.12646386251819003 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1512 0.12473148083985863 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1456 0.12011179636430831 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1363 0.1124398203602694 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1361 0.11227483162899975 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1350 0.11136739360701664 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1318 0.10872757390670218 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1311 0.10815011334725838 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1294 0.1067477091314663 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 1283 0.10584027110948323 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1264 0.10427287816242152 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1254 0.10344793450607324 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1231 0.10155056409647222 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 8.249436563482714E-5 0.0 0.0 0.0 6 0.0 8.249436563482714E-5 0.0 0.0 0.0 7 0.0 8.249436563482714E-5 0.0 0.0 0.0 8 0.0 8.249436563482714E-5 0.0 0.0 0.0 9 0.0 8.249436563482714E-5 0.0 8.249436563482714E-5 0.0 10-11 0.0 8.249436563482714E-5 0.0 5.362133766263764E-4 0.0 12-13 0.0 8.249436563482714E-5 0.0 8.249436563482714E-4 0.0 14-15 0.0 8.249436563482714E-5 0.0 0.0011961683017049935 0.0 16-17 0.0 8.249436563482714E-5 0.0 0.0016911344955139564 0.0 18-19 0.0 8.249436563482714E-5 0.0 0.0025573253346796417 0.0 20-21 0.0 8.249436563482714E-5 0.0 0.004330954195828425 0.0 22-23 0.0 8.249436563482714E-5 0.0 0.006558302067968758 0.0 24-25 0.0 8.249436563482714E-5 0.0 0.009321863316735467 0.0 26-27 0.0 8.249436563482714E-5 0.0 0.011879188651415108 0.0 28-29 0.0 8.249436563482714E-5 0.0 0.0197574005695411 0.0 30-31 0.0 8.249436563482714E-5 0.0 0.03279151033984379 0.0 32-33 0.0 8.249436563482714E-5 0.0 0.057168595384935204 0.0 34-35 0.0 8.249436563482714E-5 0.0 0.08546416279768092 0.0 36-37 0.0 1.2374154845224073E-4 0.0 0.11384222457606145 0.0 38-39 0.0 1.649887312696543E-4 0.0 0.1554193848560143 0.0 40-41 0.0 1.649887312696543E-4 0.0 0.1969552979531498 0.0 42-43 0.0 1.649887312696543E-4 0.0 0.23638760472659717 0.0 44-45 0.0 1.649887312696543E-4 0.0 0.26521938551596924 0.0 46-47 0.0 1.649887312696543E-4 0.0 0.30506416411759074 0.0 48-49 0.0 1.649887312696543E-4 0.0 0.3251927893324886 0.0 50-51 0.0 1.649887312696543E-4 0.0 0.3456101448271083 0.0 52-53 0.0 1.649887312696543E-4 0.0 0.3906520684637239 0.0 54-55 0.0 1.649887312696543E-4 0.0 0.45644132505749857 0.0 56-57 0.0 1.649887312696543E-4 0.0 0.5858749847385424 0.0 58-59 0.0 1.649887312696543E-4 0.0 0.722073182401642 0.0 60-61 0.0 1.649887312696543E-4 0.0 0.8763788933215861 0.0 62-63 0.0 1.649887312696543E-4 0.0 0.9525211928025317 0.0 64-65 0.0 1.649887312696543E-4 0.0 1.0624449350109386 0.0 66-67 0.0 1.649887312696543E-4 0.0 1.5412834803382929 0.0 68-69 0.0 1.649887312696543E-4 0.0 2.0657414098617064 0.0 70-71 0.0 1.649887312696543E-4 0.0 2.413413913829685 0.0 72-73 0.0 1.649887312696543E-4 0.0 2.8260094835522738 0.0 74-75 0.0 1.649887312696543E-4 0.0 3.161225338309394 0.0 76-77 0.0 1.649887312696543E-4 0.0 3.359253063015796 0.0 78-79 0.0 1.649887312696543E-4 0.0 3.572500998181824 0.0 80-81 0.0 1.649887312696543E-4 0.0 3.7961844706006582 0.0 82-83 0.0 1.649887312696543E-4 0.0 4.125584472580522 0.0 84-85 0.0 1.649887312696543E-4 0.0 5.0901085955829215 0.0 86-87 0.0 1.649887312696543E-4 0.0 7.100248803006755 0.0 88-89 0.0 1.649887312696543E-4 0.0 9.1746108740773 0.0 90-91 0.0 1.649887312696543E-4 0.0 11.064185566125833 0.0 92-93 0.0 1.649887312696543E-4 0.0 12.737501278662668 0.0 94-95 0.0 2.0623591408706784E-4 0.0 13.914118415712206 0.0 96-97 0.0 2.474830969044814E-4 0.0 14.78303156894384 0.0 98-99 0.0 2.474830969044814E-4 0.0 15.450617222843679 0.0 100-101 0.0 2.474830969044814E-4 0.0 15.824069216072543 0.0 102-103 0.0 2.474830969044814E-4 0.0 16.174711517203377 0.0 104-105 0.0 2.474830969044814E-4 0.0 16.552040745617074 0.0 106-107 0.0 2.474830969044814E-4 0.0 17.090687706029676 0.0 108-109 0.0 2.474830969044814E-4 0.0 17.68402843085817 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAGTCG 55 0.0010127891 41.7877 5 TACCTGG 710 0.0 36.471855 2 GTACCCG 135 7.788003E-8 34.136078 1 ACCTGGG 800 0.0 31.604584 3 ATAACGC 165 1.3231329E-8 31.34339 3 TCTCGGG 440 0.0 30.046814 114-115 AGTACGC 640 0.0 29.671373 2 TATAACG 175 2.3137545E-8 29.594307 2 GTACGCA 625 0.0 29.420996 3 TACCCGG 170 5.9257036E-7 27.079758 2 ATATCGT 85 0.008548207 27.040228 6 GAGTACG 730 0.0 26.829554 1 TACGCGC 155 8.956729E-6 25.949896 6 GTACGCG 155 8.959629E-6 25.948812 5 ACGCGCT 160 1.1483944E-5 25.131626 7 GTAGGAC 210 1.374483E-7 24.62695 3 TACGCAG 760 0.0 24.19086 4 ACCGTGT 225 2.6879388E-7 22.974613 8 TAACGCA 230 3.3130345E-7 22.481724 4 ATCTCGG 1865 0.0 22.345108 114-115 >>END_MODULE