##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003843_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1212204 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.988006144180353 31.0 31.0 34.0 30.0 34.0 2 32.13110417058515 33.0 31.0 34.0 30.0 34.0 3 32.24343509838278 34.0 31.0 34.0 30.0 34.0 4 35.79072746831391 37.0 35.0 37.0 35.0 37.0 5 35.71723901257544 37.0 35.0 37.0 35.0 37.0 6 35.9042875621595 37.0 35.0 37.0 35.0 37.0 7 35.90138953509475 37.0 35.0 37.0 35.0 37.0 8 35.92434359233264 37.0 35.0 37.0 35.0 37.0 9 37.551914529237656 39.0 37.0 39.0 35.0 39.0 10-11 37.45634521912153 39.0 37.0 39.0 34.5 39.0 12-13 37.477020369508764 39.0 37.0 39.0 35.0 39.0 14-15 38.44192561648039 40.0 38.0 41.0 34.0 41.0 16-17 38.61186978429373 40.0 38.0 41.0 34.5 41.0 18-19 38.704582726999746 40.0 38.0 41.0 34.5 41.0 20-21 38.659528841680114 40.0 38.0 41.0 34.0 41.0 22-23 38.576431854704325 40.0 38.0 41.0 34.0 41.0 24-25 38.34722620944989 40.0 38.0 41.0 33.5 41.0 26-27 37.99552715549528 39.5 37.5 41.0 33.5 41.0 28-29 37.923592068661705 39.5 37.0 41.0 33.0 41.0 30-31 37.931044197181336 40.0 37.0 41.0 33.0 41.0 32-33 37.675380958980504 40.0 37.0 41.0 33.0 41.0 34-35 37.504191538717905 39.0 36.5 41.0 32.5 41.0 36-37 37.313372171680676 39.5 36.0 41.0 32.0 41.0 38-39 37.11682769566839 39.0 36.0 40.0 31.0 41.0 40-41 37.40621916773084 39.5 37.0 41.0 32.5 41.0 42-43 37.3051054113004 40.0 37.0 41.0 32.0 41.0 44-45 37.02945048853164 40.0 36.5 41.0 31.0 41.0 46-47 36.88394733889675 40.0 36.5 41.0 31.0 41.0 48-49 36.49238329522093 39.0 36.0 41.0 30.0 41.0 50-51 36.040522882287135 39.0 35.0 40.0 28.5 41.0 52-53 36.12410246130189 39.0 36.0 40.5 29.0 41.0 54-55 36.01169068902594 39.0 35.0 41.0 28.5 41.0 56-57 35.53536244724485 38.5 34.5 40.0 27.5 41.0 58-59 35.26037737872503 38.0 34.5 40.0 28.0 41.0 60-61 34.5168989708003 38.0 34.0 40.0 26.0 41.0 62-63 34.50422701129513 37.5 34.0 40.0 26.0 41.0 64-65 34.05313998303916 37.0 33.5 39.5 26.0 41.0 66-67 33.59511559110513 36.5 33.0 39.0 25.5 40.0 68-69 33.05397152624476 36.0 32.5 38.5 24.5 40.0 70-71 32.31364069084081 35.0 31.5 37.5 22.5 39.5 72-73 31.71681994119802 34.5 31.0 37.0 21.5 39.0 74-75 31.391648187928766 34.0 31.0 36.0 20.0 39.0 76-77 30.580575546690163 34.0 30.0 35.0 20.0 37.0 78-79 31.025176455448094 34.0 31.0 35.0 20.0 37.0 80-81 30.57556525139333 34.0 30.5 35.0 19.0 37.0 82-83 30.25120606762558 34.0 30.0 35.0 14.5 36.5 84-85 29.99199639664611 34.0 30.0 35.0 15.5 36.0 86-87 30.02880950731065 34.0 31.0 35.0 12.0 36.0 88-89 29.705464591768383 34.0 30.0 35.0 7.0 35.5 90-91 29.338004989259233 34.0 29.0 35.0 4.0 35.0 92-93 29.093049519717802 34.0 29.0 35.0 2.0 35.0 94-95 28.588275570778514 34.0 29.0 35.0 2.0 35.0 96-97 28.01141474537289 34.0 28.5 35.0 2.0 35.0 98-99 27.993052736998063 34.0 29.0 35.0 2.0 35.0 100-101 26.98243859944366 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.02361445311257171 1101 2 0.1296766595103449 1101 3 0.08930039491414732 1101 4 0.043610112508361 1101 5 0.03272877719581402 1101 6 0.13432969623271873 1101 7 0.13023357484419051 1101 8 0.07330593492186921 1101 9 0.015029931657572604 1101 10-11 0.21279765416282004 1101 12-13 0.23748210685833016 1101 14-15 0.15215106125420874 1101 16-17 0.19923891649302305 1101 18-19 0.16687322103803126 1101 20-21 0.0012810808499779114 1101 22-23 0.014836704781934884 1101 24-25 0.09259103532386348 1101 26-27 0.025447916599148357 1101 28-29 0.14959938967015063 1101 30-31 0.23119028031042177 1101 32-33 0.07185510878993995 1101 34-35 0.03992127519147459 1101 36-37 0.12287113757729884 1101 38-39 0.09308936186679517 1101 40-41 0.24828314071927338 1101 42-43 0.19665807587897888 1101 44-45 0.17579266560990447 1101 46-47 0.1228285130981206 1101 48-49 0.13297385083707525 1101 50-51 -0.08677009916623746 1101 52-53 -0.026008368904463453 1101 54-55 0.21077451106518197 1101 56-57 -0.10621440431192042 1101 58-59 0.0774900329316921 1101 60-61 -0.12806777399944735 1101 62-63 -0.10896847164268308 1101 64-65 0.035417425157476146 1101 66-67 -0.10792317431751286 1101 68-69 -0.14036334319047228 1101 70-71 -0.3191091024556414 1101 72-73 -0.1670606717554115 1101 74-75 -0.28066398414668114 1101 76-77 -0.18722200606982042 1101 78-79 -0.0440573830037323 1101 80-81 -0.382027356098785 1101 82-83 0.036224952853679326 1101 84-85 -0.04537516584206713 1101 86-87 0.12373206578784846 1101 88-89 0.13583703884939524 1101 90-91 0.1161647152328591 1101 92-93 0.15883896062564062 1101 94-95 0.06265817546094965 1101 96-97 -0.08160833720962302 1101 98-99 0.03386211143392259 1101 100-101 0.08246182626092846 1102 1 -0.06289883227376336 1102 2 0.09672406109536524 1102 3 0.058418952365485666 1102 4 -0.07358469013556146 1102 5 0.03724287982062435 1102 6 0.05433502757381348 1102 7 0.014927540081743018 1102 8 0.07098750303764234 1102 9 0.06376084449189534 1102 10-11 0.08661322834299057 1102 12-13 0.053414048080298926 1102 14-15 -0.0185868910548308 1102 16-17 0.057369123976890535 1102 18-19 0.08851478305082594 1102 20-21 0.03191173991947949 1102 22-23 0.03166375011412015 1102 24-25 0.010457692169637767 1102 26-27 -0.05264658492923502 1102 28-29 -0.05746095444687427 1102 30-31 0.02765844220781588 1102 32-33 0.07251275777374389 1102 34-35 -0.026766085496554126 1102 36-37 -0.0784597685928432 1102 38-39 -0.11146402432398617 1102 40-41 0.12898386132718542 1102 42-43 0.07578570011764185 1102 44-45 -0.09824265101739371 1102 46-47 -0.13197100918566917 1102 48-49 -0.171144084039895 1102 50-51 0.047029025020691506 1102 52-53 -0.00820712621094799 1102 54-55 -0.04727730465982205 1102 56-57 0.004439859971782312 1102 58-59 0.10183619831200019 1102 60-61 -0.3619680884433478 1102 62-63 -0.1628869196612328 1102 64-65 -0.23495785565863514 1102 66-67 -0.2368758639264783 1102 68-69 -0.2870839546111128 1102 70-71 -0.39878546900638767 1102 72-73 -0.21418604420395226 1102 74-75 -0.27108861494253844 1102 76-77 -0.19436758149513977 1102 78-79 -0.08357770260359842 1102 80-81 -0.1340770287649633 1102 82-83 -0.10395073588017567 1102 84-85 -0.21438569348700298 1102 86-87 -0.14247284360565615 1102 88-89 -0.229934172960931 1102 90-91 -0.3364959571825388 1102 92-93 -0.37790081907072093 1102 94-95 -0.4798855757337783 1102 96-97 -0.2161212297603008 1102 98-99 -0.2417208724685871 1102 100-101 -0.27022567823300747 1103 1 -0.098510404363374 1103 2 -0.03198725275889558 1103 3 -0.20795801880915832 1103 4 -0.013485983933982482 1103 5 0.005685078360350815 1103 6 0.031534943680100014 1103 7 0.08918920982888068 1103 8 0.09215690280431232 1103 9 0.09749595008000256 1103 10-11 0.11222211321197761 1103 12-13 -0.05206591574054187 1103 14-15 -0.07760251625965253 1103 16-17 0.013685731000371959 1103 18-19 -0.21604759323872003 1103 20-21 -0.030341003011812973 1103 22-23 -0.08018296033457517 1103 24-25 -0.05051272218667435 1103 26-27 -0.16884855905325935 1103 28-29 -0.19728621956412695 1103 30-31 -0.1299208308006925 1103 32-33 -0.15747826297684497 1103 34-35 -0.06819811192307412 1103 36-37 -0.21036002682819088 1103 38-39 -0.013258563367337217 1103 40-41 -0.05186707002415858 1103 42-43 0.01769814915876111 1103 44-45 0.18883345631413562 1103 46-47 -0.06254371488912369 1103 48-49 -0.06604033110689045 1103 50-51 -0.015235515464581795 1103 52-53 0.05506315575059517 1103 54-55 -0.02905663796693858 1103 56-57 -0.12032844917656149 1103 58-59 -0.08779014693686804 1103 60-61 -0.12930885423568128 1103 62-63 -0.07830370102633566 1103 64-65 -0.12200119200690551 1103 66-67 -0.11446903183189505 1103 68-69 -0.33845640339006877 1103 70-71 -0.3829390600507345 1103 72-73 -0.0366633339125535 1103 74-75 0.09011217942609306 1103 76-77 -0.01554714026662296 1103 78-79 0.06901415446247938 1103 80-81 0.011822010389806792 1103 82-83 0.15562199604007176 1103 84-85 0.11637970559803534 1103 86-87 0.2810619657327642 1103 88-89 -0.07795480557199141 1103 90-91 -0.08150493643195134 1103 92-93 -0.051320602257892034 1103 94-95 -0.1295536056733475 1103 96-97 -0.22336820499854682 1103 98-99 -0.16266382474092822 1103 100-101 0.006457111757423917 1104 1 -0.022588021661331226 1104 2 -0.04979994853768943 1104 3 -0.011244562773661926 1104 4 -0.00772479596940201 1104 5 -0.10475278885717643 1104 6 -4.5086711926956013E-4 1104 7 -0.10850123531918854 1104 8 -0.06926049335884699 1104 9 0.021853795624288352 1104 10-11 0.029734685385570003 1104 12-13 0.08729895667518406 1104 14-15 -0.10682541200797147 1104 16-17 -0.021371161259914118 1104 18-19 0.01944657361138269 1104 20-21 -0.07394528009066903 1104 22-23 -0.06771830634450282 1104 24-25 0.1915291342619625 1104 26-27 -0.07406368291245258 1104 28-29 -0.1812945840809661 1104 30-31 -0.043257494137357355 1104 32-33 0.11160900568669518 1104 34-35 0.02749983419860058 1104 36-37 -0.22828828501069154 1104 38-39 -0.12425942691056946 1104 40-41 0.13064152307765653 1104 42-43 0.10296076789595787 1104 44-45 0.1481034236349501 1104 46-47 0.16496098380201119 1104 48-49 4.635040410150282E-4 1104 50-51 -0.16506746317788412 1104 52-53 0.13967515392190677 1104 54-55 0.1498407358456859 1104 56-57 -0.08065795018404032 1104 58-59 -0.02569249882226643 1104 60-61 -0.13181270631579878 1104 62-63 -0.03033810729802866 1104 64-65 0.04019042993049027 1104 66-67 0.057480205371582827 1104 68-69 -0.07567934993505077 1104 70-71 -0.17351924258007045 1104 72-73 -0.5517579850241532 1104 74-75 -0.3731825766652719 1104 76-77 -0.22371989971057005 1104 78-79 -0.006066273584050208 1104 80-81 -0.1149901604901622 1104 82-83 0.031010590496464374 1104 84-85 0.057926294602250294 1104 86-87 0.029281542765897228 1104 88-89 -0.006599889301817541 1104 90-91 -0.08038740989069382 1104 92-93 -8.412686887382392E-5 1104 94-95 0.02536478102470241 1104 96-97 -0.18107236524507897 1104 98-99 0.15504516118840073 1104 100-101 0.30703619369243995 1105 1 -0.002698075541875511 1105 2 -0.08628805059956335 1105 3 0.019978312711508295 1105 4 -0.0787521292918143 1105 5 -0.18111085701852403 1105 6 0.0041920309662515365 1105 7 0.09110243969432474 1105 8 0.025767429537928876 1105 9 -0.029144386521430476 1105 10-11 -0.025390517280619918 1105 12-13 0.007583590074574431 1105 14-15 -0.17885584059578008 1105 16-17 0.03849520188513367 1105 18-19 -0.061030326537647284 1105 20-21 -0.01761226883962763 1105 22-23 0.056374813650258204 1105 24-25 0.09237419084472265 1105 26-27 0.02961133059862675 1105 28-29 -0.09038549317187261 1105 30-31 0.1487676937894733 1105 32-33 0.1280147311088129 1105 34-35 -0.046196960809659515 1105 36-37 0.08278588671921483 1105 38-39 0.031666614261375514 1105 40-41 0.12685023240111803 1105 42-43 0.35660875924886426 1105 44-45 0.42196071388568157 1105 46-47 0.1829470960504196 1105 48-49 -0.01356345260889924 1105 50-51 -0.0741993149326916 1105 52-53 -0.04862834585699716 1105 54-55 0.008920180990713789 1105 56-57 -0.10702911999782572 1105 58-59 0.2393334588266427 1105 60-61 0.06868761303697113 1105 62-63 0.051567691820885386 1105 64-65 -0.2577779950871246 1105 66-67 -0.33390300158539077 1105 68-69 -0.5355964819833261 1105 70-71 -0.32416777437614286 1105 72-73 -0.3881429115730164 1105 74-75 -0.5768518582034048 1105 76-77 -0.4187871275646806 1105 78-79 -0.33019296656320307 1105 80-81 -0.4251267452169074 1105 82-83 -0.1380498572525113 1105 84-85 -0.18424546806131303 1105 86-87 -0.3335154962606488 1105 88-89 -0.28131288122792597 1105 90-91 -0.24387146681737448 1105 92-93 -0.40240721361819354 1105 94-95 -0.5629567335263239 1105 96-97 -0.6148598891965378 1105 98-99 -0.5753965282696818 1105 100-101 -0.5263476355930301 1106 1 0.04105885777502394 1106 2 -0.05407637386674935 1106 3 0.0050023482100911565 1106 4 -0.03261218927784881 1106 5 0.019123335018946364 1106 6 0.13548337407812738 1106 7 0.07185349766712079 1106 8 0.10906994812948767 1106 9 0.1142263353652524 1106 10-11 0.20686019145843915 1106 12-13 0.04951218204629981 1106 14-15 -0.042551966155578214 1106 16-17 -0.024017637590603158 1106 18-19 0.08910936695838956 1106 20-21 0.20378137921493789 1106 22-23 0.13420257978690842 1106 24-25 0.19757571925590867 1106 26-27 0.07515551893833106 1106 28-29 -0.1455233377834091 1106 30-31 0.14764809150506863 1106 32-33 0.15065329473098643 1106 34-35 0.015524966960576592 1106 36-37 -0.05490465110074183 1106 38-39 0.12437790067412635 1106 40-41 0.2583778218665884 1106 42-43 0.36578128071646887 1106 44-45 0.4458626819205236 1106 46-47 0.3904022092432413 1106 48-49 0.3235395508012715 1106 50-51 0.12380594411657597 1106 52-53 0.2227981712554552 1106 54-55 0.22102832019116647 1106 56-57 0.10754419854443142 1106 58-59 0.04195476619342031 1106 60-61 -0.041074497156543543 1106 62-63 0.2575231542474441 1106 64-65 0.2269378929937318 1106 66-67 0.12061443955844453 1106 68-69 -0.0481586250459074 1106 70-71 -0.09871685198820757 1106 72-73 -0.08382679340875043 1106 74-75 0.1525022648090406 1106 76-77 -0.012956978421332366 1106 78-79 0.18495761480825834 1106 80-81 0.09196516488621853 1106 82-83 0.2714061598394011 1106 84-85 0.12093346287257845 1106 86-87 0.26495814686355246 1106 88-89 0.43253115509238427 1106 90-91 0.23511287929380487 1106 92-93 0.20635855325801344 1106 94-95 0.009827686540237579 1106 96-97 -0.4001427685260097 1106 98-99 -0.08801002923521395 1106 100-101 -0.08788467491264029 1107 1 -0.03193260965760203 1107 2 0.04618833284384749 1107 3 0.021507797225886804 1107 4 0.07494955215438637 1107 5 -0.02521855904205239 1107 6 0.057848979101926545 1107 7 0.13044801767500758 1107 8 0.10515918808330582 1107 9 0.03055155853553515 1107 10-11 0.12821906617331535 1107 12-13 0.030085103113016487 1107 14-15 0.0758444462236767 1107 16-17 0.018789520754701527 1107 18-19 -0.13832192193800807 1107 20-21 -0.0198834711131326 1107 22-23 0.0019037850472543028 1107 24-25 0.2583808219001611 1107 26-27 0.3538209324721606 1107 28-29 -0.044639104728865675 1107 30-31 0.3714836635813299 1107 32-33 0.21111343384280445 1107 34-35 0.08610344968423789 1107 36-37 0.23759274407258602 1107 38-39 0.28185903328092365 1107 40-41 0.3410652285856344 1107 42-43 0.23462825770950957 1107 44-45 0.6216867223586959 1107 46-47 0.5803433542405614 1107 48-49 0.5944362447834166 1107 50-51 0.6033228543697291 1107 52-53 0.26321874516212773 1107 54-55 0.44895945420597627 1107 56-57 0.5608227601168991 1107 58-59 0.6147807919319206 1107 60-61 0.22780169613567836 1107 62-63 0.5562087727881746 1107 64-65 0.37876056765792754 1107 66-67 0.24530291636845192 1107 68-69 0.305532836754665 1107 70-71 0.15356589138708898 1107 72-73 0.25458595193775935 1107 74-75 0.10439041214366895 1107 76-77 0.19500323109121354 1107 78-79 0.24494254905398805 1107 80-81 0.20350454704949072 1107 82-83 0.2285462009534207 1107 84-85 0.014306291010335315 1107 86-87 0.040422087614306434 1107 88-89 -0.02451736508446345 1107 90-91 -0.2152651725774497 1107 92-93 -0.23211473670847482 1107 94-95 -0.36859378849566937 1107 96-97 -0.2654697094738836 1107 98-99 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-0.2288521718550527 2316 94-95 -0.22983929252215063 2316 96-97 -0.4491640469344951 2316 98-99 -0.5048710557391516 2316 100-101 -0.8825235383602106 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 5.0 5 14.0 6 34.0 7 90.0 8 252.0 9 491.0 10 846.0 11 1359.0 12 2312.0 13 3348.0 14 4463.0 15 5713.0 16 7401.0 17 10057.0 18 9569.0 19 7129.0 20 6707.0 21 6659.0 22 7097.0 23 7528.0 24 8499.0 25 9739.0 26 11854.0 27 14561.0 28 18356.0 29 22647.0 30 28837.0 31 36353.0 32 46395.0 33 61005.0 34 83488.0 35 122648.0 36 196218.0 37 252908.0 38 183751.0 39 33849.0 40 16.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.92814469120558 18.412520490822256 12.863036290009724 15.79629852796244 2 15.101182177417357 29.74896260425518 32.69417655939348 22.455678658933977 3 36.387422239133286 23.06896770876064 24.635616519763246 15.907993532342827 4 13.121388809144335 15.99186275577378 44.9208219078637 25.96592652721819 5 11.624116072872223 43.122527231390094 33.77443070638275 11.478925989354927 6 27.385984537256103 44.12037907810897 13.164120890543177 15.329515494091753 7 29.707788458048316 34.88909457484054 19.172845494652716 16.230271472458433 8 23.066167080788382 26.415273336831095 18.932869385021004 31.585690197359522 9 19.589772018571132 31.17825052548911 21.517912826553946 27.714064629385817 10-11 27.142337428353642 23.985195561143176 26.570940204784016 22.301526805719167 12-13 36.72265559262302 22.483550623492416 21.048973605102773 19.74482017878179 14-15 23.48642872787705 28.174243889802998 31.312942154780522 17.026385227539436 16-17 18.866172690405243 28.378391755843076 30.114155703165473 22.641279850586205 18-19 18.14603812559602 38.714853275521286 25.8984461361289 17.240662462753793 20-21 24.457403050745235 25.309667926088093 23.814037949300555 26.418891073866117 22-23 19.65197277025979 29.83738710646063 24.09371689913579 26.41692322414379 24-25 29.08095502077208 28.341104302576138 24.185450633721718 18.392490042930067 26-27 27.805069004476824 22.537117466215506 32.53850757941692 17.11930594989075 28-29 19.69713283162115 37.12913540247325 26.818954176376458 16.35477758952914 30-31 23.651694825410562 27.328000105603024 24.657893640429126 24.362411428557284 32-33 23.44800759976026 31.013407240974356 25.196315452749612 20.342269706515772 34-35 28.12110837260624 25.0061564797723 27.714636334574255 19.1580988130472 36-37 25.887814725106345 23.746473143911405 31.489941214595813 18.875770916386436 38-39 21.414407123457497 29.371427345132854 31.580422212436748 17.6337433189729 40-41 20.644039313729817 31.00371943490329 26.78071503733253 21.57152621403436 42-43 18.283965840370854 34.080587253215825 21.82092555747018 25.814521348943142 44-45 25.21901200791397 23.3553266657536 24.092929736294526 27.332731590037902 46-47 29.071172323556237 30.836115269291263 23.754192497455936 16.338519909696565 48-49 28.355968032998195 28.663841196184585 24.781521010569733 18.198669760247487 50-51 17.96126796873389 29.91218264689504 34.63023078350898 17.496318600862086 52-53 24.622316743290877 35.571150798593955 21.45623675437822 18.350295703736947 54-55 19.009472913966672 30.245884937464577 24.68823033955814 26.05641180901061 56-57 25.704987498561334 24.90791507519633 26.661457905772856 22.725639520469482 58-59 33.718875376102524 24.75156814231134 21.44413469812384 20.085421783462294 60-61 22.959732113909336 29.236394295458428 32.05558355889542 15.748290031736815 62-63 19.721136902129793 27.50528818548414 30.25011219863118 22.523462713754892 64-65 18.774716530118095 38.22637544561486 24.92714313139007 18.071764892876974 66-67 24.06139577338727 25.694212042045773 23.012933700702206 27.231458483864753 68-69 20.262709682530254 30.457274605720926 23.099241479016893 26.180774232731927 70-71 28.889416954111546 28.52513446067007 23.151204463141255 19.434244122077125 72-73 27.54724670248581 23.048281596863887 31.005542810144522 18.398928890505783 74-75 20.185093371632874 36.84531170212151 25.77934811033502 17.19024681591059 76-77 23.657210832892673 27.21434717294301 23.82574779511911 25.302694199045206 78-79 23.34116346552439 31.10388364932282 24.122141812699663 21.432811072453127 80-81 27.68297496812599 26.1271190390651 25.999581750288424 20.190324242520486 82-83 25.04455750816183 24.750504765106346 30.025676508984716 20.179261217747108 84-85 20.770164548540233 29.478165856910042 29.74346959710707 20.00819999744266 86-87 20.64226049293596 30.375992575023204 25.38512942147056 23.59661751057028 88-89 18.80698827999647 32.56077168720369 21.706631161003827 26.925608871796015 90-91 24.628503876857895 21.63203384279566 24.572737179640537 29.16672510070591 92-93 27.257435760367827 28.932325083453676 24.677538639473124 19.132700516705377 94-95 26.320187861981008 27.60746976591087 25.816337937073826 20.256004435034296 96-97 16.85570497483706 29.355457470505737 34.6600940516459 19.128743503011304 98-99 23.04012626422009 35.39131471464113 21.585373349454628 19.98318567168415 100-101 17.567719757383344 31.80967059133169 23.378976935906582 27.243632715378386 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 51.0 1 41.5 2 52.5 3 65.5 4 59.0 5 64.5 6 87.5 7 175.0 8 321.0 9 437.5 10 465.0 11 497.5 12 571.0 13 664.0 14 761.5 15 854.5 16 970.0 17 1084.5 18 1194.0 19 1307.5 20 1401.0 21 1532.5 22 1744.0 23 2005.0 24 2342.5 25 2926.0 26 3722.0 27 4750.5 28 6335.0 29 8315.5 30 10508.5 31 12725.5 32 14570.5 33 16375.5 34 18160.5 35 19564.0 36 20788.0 37 22141.5 38 23650.0 39 24436.5 40 24572.0 41 24941.5 42 25649.0 43 26908.0 44 27994.0 45 29918.5 46 38651.0 47 96515.0 48 220402.0 49 211653.5 50 91125.0 51 40840.0 52 25372.5 53 19548.5 54 16610.5 55 14351.0 56 12106.0 57 9709.5 58 7299.0 59 5614.0 60 4315.5 61 3205.5 62 2332.0 63 1703.5 64 1178.5 65 753.0 66 476.5 67 311.5 68 198.0 69 113.5 70 63.0 71 35.5 72 17.5 73 7.5 74 5.5 75 4.0 76 2.0 77 2.0 78 2.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05659113482549141 2 0.002804808431584123 3 0.05254891090938489 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.002433583786227401 16-17 0.0 18-19 0.0 20-21 0.011507964006058387 22-23 0.0 24-25 0.0 26-27 0.0059808415085249676 28-29 0.012745379490580792 30-31 0.009693087962092188 32-33 0.004990909120907042 34-35 0.005485875314716005 36-37 0.02091232168842868 38-39 0.054240045404898844 40-41 0.004743426024002561 42-43 0.015550187922164916 44-45 0.014024042157920615 46-47 0.005650864045985659 48-49 0.001361157032974648 50-51 0.002392336603409987 52-53 9.074380219830985E-4 54-55 9.074380219830985E-4 56-57 0.011837941468597695 58-59 0.008331930929117541 60-61 0.003877235184836875 62-63 0.005609616863168246 64-65 0.09045507191858797 66-67 0.043268294775466835 68-69 0.030687904016155693 70-71 0.01917994001009731 72-73 2.4748309690448146E-4 74-75 4.1247182817413574E-4 76-77 5.7746055944379E-4 78-79 5.362133766263764E-4 80-81 7.836964735308577E-4 82-83 5.362133766263764E-4 84-85 7.012021078960307E-4 86-87 0.006517054885151345 88-89 2.4748309690448146E-4 90-91 9.074380219830985E-4 92-93 3.7122464535672213E-4 94-95 0.0030935387113060174 96-97 0.008579414026022022 98-99 0.011755447102962868 100-101 0.013900300609468374 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1212204.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.14838241600558 #Duplication Level Percentage of deduplicated Percentage of total 1 85.64072172734177 33.52695724564694 2 8.75999972325304 6.858796382600262 3 2.2104270975236093 2.5960393594966655 4 0.9317855675918209 1.4591159091919768 5 0.5025214149868455 0.9836450263068635 6 0.33573039865777987 0.7885981221199665 7 0.251779709190455 0.6899737837985026 8 0.1787242975619425 0.559741371838952 9 0.15119830424893468 0.5327252131849972 >10 0.8971429649380783 6.716501784709389 >50 0.07771237287742189 2.0680027194165564 >100 0.04745419566120049 3.5835923491587076 >500 0.004135916134937009 1.0916441070264449 >1k 0.006748073693844595 5.984527639347316 >5k 0.0015237585760294245 4.431512634940608 >10k+ 0.0023944777623319527 28.12862635121584 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 122012 10.06530253983653 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 40513 3.3420942349637524 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 26905 2.219510907405024 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 23904 1.971945316134908 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 20075 1.6560743901191548 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 19165 1.581004517391462 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 18606 1.5348901670015938 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 17615 1.45313825065748 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 16951 1.398361991875955 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 13588 1.1209334402460311 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 10744 0.8863194643805827 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 9967 0.8222213422823221 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 8991 0.7417068414227308 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 7552 0.6229974492742146 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 7011 0.5783679974657732 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 6690 0.5518873060969935 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6663 0.5496599582248533 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 5128 0.4230311069753936 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4305 0.3551382440579308 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3958 0.32651269918264586 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 3926 0.32387287948233134 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3591 0.29623726699466424 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3454 0.28493553890269296 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3213 0.2650543967846996 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3166 0.2611771615998627 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2904 0.23956363780353804 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2745 0.2264470336676005 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 2675 0.22067242807316262 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2654 0.21894004639483122 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2628 0.21679519288832572 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2398 0.1978214887923155 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 2174 0.17934275089011423 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 2118 0.1747230664145639 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 1998 0.16482374253838464 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1995 0.16457625944148016 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 1797 0.14824237504578439 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 1794 0.14799489194887988 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1769 0.14593253280800922 No Hit GAGTAGGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1646 0.13578572583492549 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1645 0.13570323146929064 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 1552 0.12803125546525174 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 1489 0.1228341104302576 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1461 0.12052426819248246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1418 0.11697701047018488 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 1335 0.11012997812249424 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1261 0.10402539506551703 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.537190109915493E-4 0.0 12-13 0.0 0.0 0.0 7.424492907134443E-4 0.0 14-15 0.0 0.0 0.0 0.0010724267532527527 0.0 16-17 0.0 0.0 0.0 0.0016498873126965428 0.0 18-19 0.0 0.0 0.0 0.0027223140659492955 0.0 20-21 0.0 0.0 0.0 0.004578437292732907 0.0 22-23 0.0 0.0 0.0 0.006847032347690653 0.0 24-25 0.0 0.0 0.0 0.009610593596457362 0.0 26-27 0.0 0.0 0.0 0.012209166113954416 0.0 28-29 0.0 0.0 0.0 0.020541097043071957 0.0 30-31 0.0 0.0 0.0 0.03489511666353188 0.0 32-33 0.0 0.0 0.0 0.06129331366667656 0.0 34-35 0.0 0.0 0.0 0.0915274986718407 0.0 36-37 0.0 0.0 0.0 0.12147295339728295 0.0 38-39 0.0 0.0 0.0 0.1661848995713593 0.0 40-41 0.0 0.0 0.0 0.21077310419698334 0.0 42-43 0.0 0.0 0.0 0.253422691230189 0.0 44-45 0.0 0.0 0.0 0.28283193257900485 0.0 46-47 0.0 0.0 0.0 0.32519278933248863 0.0 48-49 0.0 1.649887312696543E-4 0.0 0.3468475603116307 0.0 50-51 0.0 2.0623591408706784E-4 0.0 0.36821360101105094 0.0 52-53 0.0 2.474830969044814E-4 0.0 0.41639031054179 0.0 54-55 0.0 2.474830969044814E-4 0.0 0.4869642403423846 0.0 56-57 0.0 2.474830969044814E-4 0.0 0.6241111232102847 0.0 58-59 0.0 2.474830969044814E-4 0.0 0.7680637912430581 0.0 60-61 4.124718281741357E-5 2.474830969044814E-4 0.0 0.9311139049202939 0.0 62-63 8.249436563482714E-5 2.474830969044814E-4 0.0 1.0116284057798852 0.0 64-65 8.249436563482714E-5 2.474830969044814E-4 0.0 1.1263368211951124 0.0 66-67 8.249436563482714E-5 2.474830969044814E-4 0.0 1.6243140593497465 0.0 68-69 8.249436563482714E-5 2.474830969044814E-4 0.0 2.1766550844577317 0.0 70-71 8.249436563482714E-5 2.474830969044814E-4 0.0 2.545116168565687 0.0 72-73 8.249436563482714E-5 2.474830969044814E-4 0.0 2.984027440925785 0.0 74-75 8.249436563482714E-5 2.474830969044814E-4 0.0 3.3442390884702577 0.0 76-77 8.249436563482714E-5 2.474830969044814E-4 0.0 3.558394461658269 0.0 78-79 8.249436563482714E-5 2.474830969044814E-4 0.0 3.788718730510706 0.0 80-81 8.249436563482714E-5 2.474830969044814E-4 0.0 4.02692121128127 0.0 82-83 8.249436563482714E-5 2.474830969044814E-4 0.0 4.376986051852658 0.0 84-85 8.249436563482714E-5 2.474830969044814E-4 0.0 5.395791467442773 0.0 86-87 8.249436563482714E-5 2.474830969044814E-4 0.0 7.503110037584433 0.0 88-89 8.249436563482714E-5 2.474830969044814E-4 0.0 9.671763168575588 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGTCG 85 7.237759E-9 44.695087 9 GTACCGG 75 6.900769E-6 38.01749 1 GTACCCG 115 1.0322947E-7 33.058685 1 TACGCAG 1295 0.0 32.99953 4 AGTACGC 1300 0.0 32.882103 2 TACCTGG 820 0.0 32.436543 2 GTACGCA 1345 0.0 31.780645 3 GACGGGT 75 3.0851483E-4 31.655104 6 GAGTACG 1405 0.0 31.117516 1 CGCAGTG 1460 0.0 29.27013 6 CCCCGGT 130 3.0468254E-7 29.220095 7 TGTACCG 65 0.005854636 29.220095 5 ACGCAGT 1475 0.0 28.972467 5 CCCGGTC 135 4.2389547E-7 28.137867 8 TACCGGG 90 8.9518254E-4 26.386871 2 TAACCGT 90 8.9669315E-4 26.379251 7 ATTCGGA 65 1.0055506E-5 25.567583 48-49 ACCTGGG 970 0.0 25.460918 3 GTAGGCA 860 0.0 25.403986 3 ACAACGC 175 1.4879697E-7 24.425695 3 >>END_MODULE