##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003842_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 450115 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.983202070581964 31.0 31.0 34.0 30.0 34.0 2 32.15096808593359 33.0 31.0 34.0 30.0 34.0 3 32.22724192706308 34.0 31.0 34.0 30.0 34.0 4 35.86113770925208 37.0 35.0 37.0 35.0 37.0 5 35.77038534596714 37.0 35.0 37.0 35.0 37.0 6 35.90021661131044 37.0 35.0 37.0 35.0 37.0 7 35.87640047543405 37.0 35.0 37.0 35.0 37.0 8 35.889137220488095 37.0 35.0 37.0 35.0 37.0 9 37.528722659764725 39.0 37.0 39.0 35.0 39.0 10-11 37.43490552414383 39.0 37.0 39.0 34.0 39.0 12-13 37.425564577941195 39.0 37.0 39.0 34.0 39.0 14-15 38.5123957210935 40.0 38.0 41.0 34.0 41.0 16-17 38.58464836763938 40.0 38.0 41.0 34.0 41.0 18-19 38.67008764426869 40.0 38.0 41.0 34.0 41.0 20-21 38.6275585128245 40.0 38.0 41.0 34.0 41.0 22-23 38.53981871299557 40.0 38.0 41.0 34.0 41.0 24-25 38.37978183353143 40.0 38.0 41.0 34.0 41.0 26-27 38.095631116492456 40.0 37.5 41.0 33.5 41.0 28-29 37.94085844728569 40.0 37.0 41.0 33.0 41.0 30-31 37.807789120558084 40.0 37.0 41.0 33.0 41.0 32-33 37.51300667607167 39.0 36.0 41.0 33.0 41.0 34-35 37.393683836352935 39.0 36.0 41.0 32.0 41.0 36-37 37.18153694055964 39.5 36.0 41.0 31.5 41.0 38-39 36.946185974695354 39.0 36.0 40.5 31.0 41.0 40-41 37.04612821167924 39.0 36.0 41.0 31.5 41.0 42-43 36.93046221521167 39.5 36.0 41.0 31.0 41.0 44-45 36.602974795330084 39.0 36.0 41.0 30.0 41.0 46-47 36.39470690823456 39.0 36.0 41.0 30.0 41.0 48-49 36.063514879530786 39.0 35.0 41.0 29.0 41.0 50-51 35.706217300023326 39.0 35.0 41.0 28.0 41.0 52-53 35.54389544894083 39.0 35.0 41.0 28.0 41.0 54-55 35.36968774646479 39.0 35.0 40.5 27.0 41.0 56-57 35.017063417126735 38.5 34.5 40.0 26.0 41.0 58-59 34.747352343290046 38.0 34.0 40.0 26.0 41.0 60-61 34.23436455128134 38.0 34.0 40.0 23.0 41.0 62-63 34.02687868655788 37.5 34.0 40.0 23.5 41.0 64-65 33.55925707874654 37.0 33.0 39.5 22.0 41.0 66-67 33.14592381946836 36.0 33.0 39.0 22.0 41.0 68-69 32.752965353298606 36.0 32.0 39.0 21.0 40.0 70-71 32.207107072636994 35.0 31.5 38.5 19.5 40.0 72-73 31.689220532530577 35.0 31.0 37.0 17.5 40.0 74-75 31.305320862446266 35.0 31.0 37.0 15.5 39.0 76-77 30.36300167734912 34.0 29.5 36.0 12.5 39.0 78-79 30.745108472279306 34.0 31.0 36.0 11.5 39.0 80-81 30.440461881963497 34.0 31.0 36.0 7.0 37.5 82-83 30.139035579796275 34.0 30.5 35.0 7.0 37.0 84-85 29.831597480643836 34.0 30.0 35.0 2.0 37.0 86-87 29.634765559912466 34.0 30.0 35.0 2.0 36.0 88-89 29.310846117103406 34.0 30.0 35.0 2.0 36.0 90-91 28.984473967763794 34.0 29.0 35.0 2.0 35.5 92-93 28.764399098008287 34.0 29.0 35.0 2.0 35.0 94-95 28.367093964875643 34.0 29.0 35.0 2.0 35.0 96-97 27.903691278895394 34.0 29.0 35.0 2.0 35.0 98-99 27.672504804327783 34.0 29.0 35.0 2.0 35.0 100-101 26.765455494706906 33.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.06374157786597934 1101 2 0.057983428934434755 1101 3 0.20459600663889432 1101 4 0.03679710515496026 1101 5 -0.055728686083867274 1101 6 0.19404934075515 1101 7 0.11032538642580647 1101 8 0.13537052247445303 1101 9 0.17618579895291475 1101 10-11 0.3315935639114329 1101 12-13 0.33855013848980064 1101 14-15 0.31692378298249935 1101 16-17 0.27423179474369164 1101 18-19 0.3817447362575379 1101 20-21 0.3551603050263381 1101 22-23 0.22179625168060113 1101 24-25 0.022108699558074818 1101 26-27 0.21916736116869373 1101 28-29 0.1972003242764373 1101 30-31 0.39544644635943627 1101 32-33 0.2992587993759699 1101 34-35 0.4852203524394483 1101 36-37 0.48178335770067093 1101 38-39 0.2894157990073438 1101 40-41 0.2686288970848665 1101 42-43 0.28006923930041694 1101 44-45 0.12594994118216363 1101 46-47 0.2954369593766728 1101 48-49 0.09959827323410764 1101 50-51 -0.21636366643961935 1101 52-53 -0.18167672936116475 1101 54-55 0.3168815859182246 1101 56-57 0.4168932450306073 1101 58-59 0.16970600065153718 1101 60-61 0.12776924643785748 1101 62-63 0.2197938383569209 1101 64-65 -0.06569977084341616 1101 66-67 0.1288561766147538 1101 68-69 0.15832360076788632 1101 70-71 0.11327075308575019 1101 72-73 0.2929121857569932 1101 74-75 0.34631559573324466 1101 76-77 0.39743724396673485 1101 78-79 0.3030792330964083 1101 80-81 0.09277372343009205 1101 82-83 0.42681601236906275 1101 84-85 0.4834655700611066 1101 86-87 0.14799652644706285 1101 88-89 0.4189137463749866 1101 90-91 0.5572926350787242 1101 92-93 0.8216309979956335 1101 94-95 0.8225465778595229 1101 96-97 0.3754016980506698 1101 98-99 0.39669121379701977 1101 100-101 0.7849085731036247 1102 1 0.02650232457304469 1102 2 0.03698240006558251 1102 3 0.06316890502296246 1102 4 0.10689333465323614 1102 5 0.031083636133651282 1102 6 0.04900304165692404 1102 7 -0.10110110997315047 1102 8 -0.06263226152361057 1102 9 0.14860000944737806 1102 10-11 -0.007588182744740379 1102 12-13 0.06258027829492363 1102 14-15 -0.04224828020215199 1102 16-17 0.07782375746232617 1102 18-19 0.030044076571037692 1102 20-21 0.03703405676633764 1102 22-23 0.09597164355859178 1102 24-25 0.2738971157052674 1102 26-27 0.12152770735279717 1102 28-29 -0.08933965030445279 1102 30-31 0.09486543806796277 1102 32-33 0.24117310065297914 1102 34-35 0.10828517117695924 1102 36-37 -0.10472938211655958 1102 38-39 -0.31717198771418964 1102 40-41 -0.18330779722003854 1102 42-43 0.11488434551599624 1102 44-45 0.33811480228486346 1102 46-47 0.01259788656906835 1102 48-49 0.04101932268033437 1102 50-51 0.32967216240501784 1102 52-53 -0.05488680017154479 1102 54-55 0.2235631812701584 1102 56-57 0.12984186210982784 1102 58-59 0.13808946612390827 1102 60-61 -0.10453118326619659 1102 62-63 -0.24148135379947178 1102 64-65 0.005437431843745344 1102 66-67 -0.09228707824211568 1102 68-69 -0.1839659350306988 1102 70-71 -0.07363235167731119 1102 72-73 0.07271912154347504 1102 74-75 -0.06841538532346547 1102 76-77 -0.14130269430113174 1102 78-79 -0.03878967690782886 1102 80-81 -0.08562003073077662 1102 82-83 -0.14642734537468982 1102 84-85 -0.0869726070253769 1102 86-87 -0.20706913959421414 1102 88-89 -0.09158676805943955 1102 90-91 -0.17380341285446121 1102 92-93 -0.13008781819996784 1102 94-95 -0.6544763598636507 1102 96-97 0.16761860781281968 1102 98-99 0.18808502847958408 1102 100-101 0.04951548468142519 1103 1 -0.10210659931146893 1103 2 0.011699580269180387 1103 3 0.15590514177748815 1103 4 0.08112205234144199 1103 5 0.2688215987890601 1103 6 0.09762785685526865 1103 7 0.08710024078852285 1103 8 0.16122034002774654 1103 9 0.21090828791008676 1103 10-11 0.2239756137161777 1103 12-13 0.02199229968307037 1103 14-15 0.19313529312909594 1103 16-17 0.13965334265995466 1103 18-19 0.08147266660770924 1103 20-21 0.01828686542580016 1103 22-23 0.12842834949763926 1103 24-25 0.2853824976503887 1103 26-27 0.3527951698877203 1103 28-29 0.1007276272660036 1103 30-31 0.05859221167536077 1103 32-33 0.045097734143276114 1103 34-35 -0.07055072495636949 1103 36-37 -0.07280021569411588 1103 38-39 -0.12685396605250787 1103 40-41 -0.11655450986731353 1103 42-43 -0.033959952172601504 1103 44-45 0.13047589982757302 1103 46-47 0.15687668794718235 1103 48-49 0.4345768277440598 1103 50-51 0.2549601924682605 1103 52-53 0.3566786896260936 1103 54-55 0.10067903806597656 1103 56-57 -0.001655503449008222 1103 58-59 -0.1825633803099933 1103 60-61 -0.23252138796455313 1103 62-63 -0.1961878529560579 1103 64-65 0.07861237306541824 1103 66-67 0.1371046882337268 1103 68-69 -0.3993583658155586 1103 70-71 -0.33717416081221074 1103 72-73 -0.11293571130465452 1103 74-75 -0.19453966321304605 1103 76-77 -0.17427391408972426 1103 78-79 -0.038473804481210294 1103 80-81 0.1344355888676425 1103 82-83 0.0040581863656008466 1103 84-85 -0.06198286473820147 1103 86-87 -0.10440408811026813 1103 88-89 -0.34569799423060843 1103 90-91 -0.14380204515941486 1103 92-93 -0.06728456438833774 1103 94-95 -0.4224729471388784 1103 96-97 -0.23815697296911154 1103 98-99 -0.10798838418006085 1103 100-101 -0.08762199472173648 1104 1 -0.08064053256981296 1104 2 0.04680456714581993 1104 3 0.08187533300358751 1104 4 -0.06124296453145206 1104 5 0.14279047767919906 1104 6 -0.06852324925646514 1104 7 -0.1996920351769802 1104 8 -0.262945389256096 1104 9 -0.0392323195139852 1104 10-11 0.1310926928312952 1104 12-13 0.06432685161407647 1104 14-15 0.029198753946495515 1104 16-17 -0.12065786843865567 1104 18-19 0.08947557249447158 1104 20-21 0.06746431199498204 1104 22-23 -0.19947988072357958 1104 24-25 -0.04067440148722312 1104 26-27 -0.5301139977163274 1104 28-29 -0.06372447247153445 1104 30-31 0.0768125903780188 1104 32-33 0.14184735919014457 1104 34-35 0.022226740360466124 1104 36-37 -0.05838795472674008 1104 38-39 -0.14411352735734795 1104 40-41 0.24411525016268598 1104 42-43 -0.028452828063116442 1104 44-45 0.5511967472216455 1104 46-47 0.4434697700619168 1104 48-49 0.34409595035373997 1104 50-51 0.1704902824570098 1104 52-53 0.36854829967484193 1104 54-55 0.1863792630378569 1104 56-57 0.28064905223153147 1104 58-59 0.33812934559927754 1104 60-61 0.3959820803519065 1104 62-63 0.25076799631277424 1104 64-65 0.5754369887384669 1104 66-67 0.37973451575528827 1104 68-69 0.18138544745777807 1104 70-71 0.43753759396843606 1104 72-73 0.3060509778592042 1104 74-75 0.1158277908551959 1104 76-77 0.3048341661502434 1104 78-79 0.236448861435548 1104 80-81 0.4524427129771311 1104 82-83 0.36675503675930443 1104 84-85 0.5744063366116272 1104 86-87 0.26969368091860346 1104 88-89 0.2183838392902011 1104 90-91 0.4049770137082227 1104 92-93 1.0003476065902923 1104 94-95 0.666390944874621 1104 96-97 0.33033055983928605 1104 98-99 0.6876973113579936 1104 100-101 0.34277733036262603 1105 1 -0.03035386271792362 1105 2 -0.1013280972911943 1105 3 -0.007280981696204947 1105 4 -0.29066843036995493 1105 5 -0.1458502294397661 1105 6 -0.10473522568582894 1105 7 0.0024699332893405312 1105 8 -0.020988071211817783 1105 9 -0.08659256796119053 1105 10-11 -0.2346353291467267 1105 12-13 0.04855503285313034 1105 14-15 -0.02342208535912249 1105 16-17 0.15960172703832853 1105 18-19 0.09812046021544063 1105 20-21 0.009846657909939438 1105 22-23 -0.2734317440776195 1105 24-25 -0.11131164467880694 1105 26-27 -0.11156108323950065 1105 28-29 -0.3588401357937201 1105 30-31 -0.7862975784386705 1105 32-33 -0.3874815546496393 1105 34-35 -1.1547421241084024 1105 36-37 -0.9513147742839934 1105 38-39 -0.6420182494468492 1105 40-41 -0.7375747084294488 1105 42-43 -0.6521886936468064 1105 44-45 -0.28099189746699693 1105 46-47 -0.43329703197662184 1105 48-49 -0.6989742041294633 1105 50-51 -0.7599275786673729 1105 52-53 -0.9682539379426132 1105 54-55 -0.9214902225490249 1105 56-57 -0.65557188263071 1105 58-59 -0.6450586620452512 1105 60-61 -0.8289890535866178 1105 62-63 -1.2229788184666432 1105 64-65 -1.3604057011803121 1105 66-67 -1.0335428771416701 1105 68-69 -0.8429366977882786 1105 70-71 -0.8643864783360655 1105 72-73 -0.963368913843297 1105 74-75 -1.0037374265361088 1105 76-77 -1.0337398504129034 1105 78-79 -0.9388328315091883 1105 80-81 -1.240760822617709 1105 82-83 -1.241313525113604 1105 84-85 -1.0162530040477122 1105 86-87 -0.8375039222029095 1105 88-89 -0.6590697759351549 1105 90-91 -0.7905905527832537 1105 92-93 -0.742505554741129 1105 94-95 -0.6672609105163652 1105 96-97 -0.46896570548958394 1105 98-99 -0.6136195782741112 1105 100-101 -0.513867836065149 1106 1 0.21922959730031977 1106 2 0.16210759744884484 1106 3 0.2335095321377878 1106 4 -0.001340367128854325 1106 5 0.1338943737830931 1106 6 0.05418237844916263 1106 7 0.2305027700178286 1106 8 0.044284047973341956 1106 9 0.10771573243407317 1106 10-11 0.1857842512728496 1106 12-13 0.35094481698204305 1106 14-15 0.30769983619757824 1106 16-17 0.18613866835788428 1106 18-19 0.2675762218451183 1106 20-21 0.10806496134562593 1106 22-23 -0.10702858202228072 1106 24-25 -0.22046336252617493 1106 26-27 0.06457756072523324 1106 28-29 0.038131588134525884 1106 30-31 0.014869949685376582 1106 32-33 -0.048612597519813505 1106 34-35 0.11267046330419106 1106 36-37 0.29049732665853867 1106 38-39 0.45232045532663534 1106 40-41 0.4270102718077027 1106 42-43 0.4435060463957612 1106 44-45 0.5167703402952455 1106 46-47 0.4521331899753349 1106 48-49 0.008955257713047615 1106 50-51 -0.2664523582356324 1106 52-53 0.2939108537873949 1106 54-55 0.20078402494261383 1106 56-57 -0.11195376162127957 1106 58-59 0.31121376118368715 1106 60-61 0.1174809981008238 1106 62-63 0.336844836139619 1106 64-65 0.05363504068651537 1106 66-67 -0.1531172158464429 1106 68-69 0.02442116174348996 1106 70-71 -0.12774920256835642 1106 72-73 -0.19571309140486548 1106 74-75 -0.34326311823571487 1106 76-77 -0.025755660689583237 1106 78-79 0.003921805158494607 1106 80-81 0.10523492076933749 1106 82-83 0.17834594585021435 1106 84-85 0.07843231063760214 1106 86-87 0.18495883243375744 1106 88-89 -0.02902416936780483 1106 90-91 -0.24337255339134245 1106 92-93 0.06954674078942702 1106 94-95 0.2776463561300311 1106 96-97 0.16036843862716665 1106 98-99 0.6009927659034489 1106 100-101 0.4599418325271287 1107 1 -0.1180759886799656 1107 2 -0.008918324420449153 1107 3 -0.007804816164515671 1107 4 0.1400981140686639 1107 5 0.09954607148298322 1107 6 0.10395236749626235 1107 7 0.059272393968171855 1107 8 0.2346162860164256 1107 9 0.14930755426685494 1107 10-11 0.14534613517209038 1107 12-13 0.03547931862497933 1107 14-15 0.16163923013697 1107 16-17 0.05131574028194308 1107 18-19 -0.06369066387959776 1107 20-21 -0.11356337016221829 1107 22-23 0.07413242564475553 1107 24-25 0.31257789340664033 1107 26-27 0.21506313940357558 1107 28-29 0.13921227451641727 1107 30-31 -0.024575103253653197 1107 32-33 -0.2826542185493892 1107 34-35 -0.28310171632340797 1107 36-37 0.20312237229563834 1107 38-39 0.5706921238187874 1107 40-41 0.15215813599171213 1107 42-43 0.18529502047389457 1107 44-45 -0.03848340194645772 1107 46-47 -0.7492833854171366 1107 48-49 -0.8219611351310547 1107 50-51 -1.362122310773053 1107 52-53 -1.0442636624594144 1107 54-55 -1.0845975011618023 1107 56-57 -0.9352323304420196 1107 58-59 -1.0614331902584482 1107 60-61 -0.8589483556779314 1107 62-63 -0.7081569893441326 1107 64-65 -1.0160181174003782 1107 66-67 -1.071050768237825 1107 68-69 -0.617506549099879 1107 70-71 -0.9265078736161811 1107 72-73 -0.7370858551872708 1107 74-75 -0.7546541368556454 1107 76-77 -0.6530868034424131 1107 78-79 -0.28038633649904554 1107 80-81 -0.3511287155942995 1107 82-83 -0.12496183071057487 1107 84-85 -0.22894994566031457 1107 86-87 0.07729910260535178 1107 88-89 -0.3193564074108899 1107 90-91 -0.1335879604742196 1107 92-93 0.028531771822649432 1107 94-95 -0.19964462598022692 1107 96-97 -0.090226669076376 1107 98-99 0.13134690975156715 1107 100-101 0.044512843845126326 1108 1 0.3217779151242226 1108 2 0.035321627513269505 1108 3 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0.1357840249426161 2316 56-57 0.21192109808690418 2316 58-59 0.42697414277740364 2316 60-61 0.07372678934661536 2316 62-63 0.365461558923009 2316 64-65 0.1855009217190613 2316 66-67 0.6321044452085474 2316 68-69 0.903773013595341 2316 70-71 0.8801632553441081 2316 72-73 0.5237537997286914 2316 74-75 0.5108895192502594 2316 76-77 0.8601141485135599 2316 78-79 0.7147888085254941 2316 80-81 0.5886860992205172 2316 82-83 0.23712540712967467 2316 84-85 0.12103332073861495 2316 86-87 0.3564992364741606 2316 88-89 0.31378727844364107 2316 90-91 0.47006740171527994 2316 92-93 0.6240248552675425 2316 94-95 0.48870135051835817 2316 96-97 0.40710525119731145 2316 98-99 0.046879410123427334 2316 100-101 -0.13275177016647177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 4.0 5 10.0 6 13.0 7 42.0 8 119.0 9 237.0 10 411.0 11 752.0 12 1362.0 13 2037.0 14 2136.0 15 2739.0 16 3625.0 17 4981.0 18 4617.0 19 3081.0 20 2670.0 21 2695.0 22 2811.0 23 2902.0 24 3253.0 25 3726.0 26 4570.0 27 5391.0 28 6595.0 29 8422.0 30 10568.0 31 13052.0 32 16300.0 33 21523.0 34 28931.0 35 41633.0 36 63842.0 37 89129.0 38 78563.0 39 17357.0 40 13.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.11325972377521 19.00495941989681 13.097225525785205 17.78455533054278 2 15.157964896689624 26.155298822483893 36.81404132415019 21.872694956676295 3 27.509919201573734 26.50832990653167 27.810218166864864 18.171532725029728 4 12.720749141885962 16.95166790709041 41.93195072370394 28.395632227319684 5 11.92828499383491 41.134154604934295 33.04355553580752 13.894004865423282 6 26.527442986792266 41.15681548048832 16.63197182942137 15.683769703298045 7 28.06127323017451 34.28901502949246 21.029959010475103 16.619752729857925 8 24.071848305433054 30.589738178021168 19.599657865212222 25.738755651333552 9 23.025671217355566 24.649922797507305 22.49602879264188 29.828377192495253 10-11 25.477822334292348 25.525921153483 27.676704842095905 21.319551670128746 12-13 31.61736445130689 23.536651744554167 25.208446730280038 19.6375370738589 14-15 23.392430655069376 27.374612590395575 29.82481865342531 19.40813810110974 16-17 19.379269742176998 29.1514390766804 29.920353687391 21.548937493751595 18-19 18.648123257389777 33.7159392599669 29.018028725992245 18.617908756651076 20-21 22.5565981349112 27.011791787299 27.31064149367083 23.12096858411897 22-23 20.153294158159582 28.92860713373249 27.296357597502862 23.62174111060507 24-25 24.671583928551595 28.483165413283274 27.714806216189196 19.13044444197594 26-27 24.404980776088365 24.971866769016454 32.3696012246426 18.253551230252583 28-29 20.204711270007568 32.0764317623545 29.752348913505987 17.966508054131943 30-31 22.365386409993203 28.34873107499789 27.749707820630753 21.536174694378158 32-33 22.130660389410576 29.422193980988272 28.40406540462418 20.04308022497698 34-35 24.742271477006618 26.050465910959236 29.689659309554173 19.517603302479973 36-37 24.147624370287573 25.51782527827345 31.75172830644857 18.58282204499041 38-39 21.85224662092697 28.210939071653797 31.71132641050805 18.22548789691118 40-41 20.342351469585175 29.593772842501437 29.60754777346015 20.456327914453233 42-43 19.50864274612579 31.248201626673804 26.289857229988478 22.953298397211924 44-45 22.87928561668109 26.539522492787178 26.789041175026355 23.792150715505375 46-47 25.00722064972517 29.653986465169808 26.43813125141636 18.900661633688664 48-49 24.81040808777381 28.941162356438348 27.12494570789682 19.123483847891023 50-51 20.190175734820375 29.603541356557283 31.64693075026105 18.55935215836129 52-53 22.625676914104805 32.412341359104666 25.686689438751426 19.2752922880391 54-55 20.08091328481943 30.048110301558612 26.907777295181308 22.963199118440652 56-57 23.697752339646453 27.30953989174969 27.54294906547455 21.449758703129305 58-59 27.152829928768536 26.836443137028365 25.56278633671198 20.447940597491122 60-61 22.580849418790656 28.956933631936298 30.491828409939213 17.970388539333833 62-63 21.1121923691326 28.43167967508937 29.57844267740223 20.8776852783758 64-65 20.239806553823065 33.507990772395004 27.198977181133436 19.05322549264849 66-67 22.36523938428167 27.412845096967192 26.29660003644882 23.92531548230232 68-69 21.03625710980912 30.118923675733818 26.018206889195312 22.826612325261753 70-71 24.89636974696553 29.171235730355804 25.73407771574591 20.198316806932755 72-73 24.074292125345746 26.357708585583683 29.657087633160415 19.91091165591016 74-75 20.780467213934216 32.56834364551281 27.594281461404307 19.05690767914866 76-77 22.875674690939064 28.293974741926203 26.34457753433301 22.485773032801724 78-79 22.30110338591625 29.92827380588717 26.321413773606494 21.44920903459009 80-81 24.298561910284352 28.02819748906381 26.832603513355597 20.840637087296248 82-83 22.839623427476464 27.220528201283013 29.608153517176262 20.33169485406426 84-85 20.89153168204429 29.295310288749715 29.282757885242148 20.53040014396385 86-87 20.69574201002011 29.806263122229755 26.43564136460081 23.062353503149335 88-89 20.29192539684303 30.412894482521136 24.830987636492896 24.46419248414294 90-91 22.504757154616133 24.960482013901 27.07551351165211 25.45924731983075 92-93 23.34877758609773 28.25031908618604 26.93320684671755 21.467696480998683 94-95 22.709307182340083 27.821236869701227 27.339681495832075 22.129774452126618 96-97 18.671550150692585 29.62150777013854 31.63022383375714 20.076718245411733 98-99 21.04803590472805 32.43390063988624 25.269952008531817 21.248111446853894 100-101 17.8791275569662 32.413216181056484 24.65608089417301 25.051575367804308 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 100.0 1 70.5 2 111.0 3 166.5 4 128.0 5 118.0 6 154.0 7 232.5 8 333.5 9 394.0 10 425.0 11 438.5 12 471.5 13 532.5 14 601.0 15 656.5 16 726.5 17 825.5 18 866.5 19 919.0 20 979.0 21 1017.5 22 1142.5 23 1319.0 24 1479.0 25 1772.5 26 2148.0 27 2662.0 28 3486.5 29 4646.0 30 5875.5 31 6933.5 32 7861.5 33 8726.5 34 9465.5 35 9975.0 36 10372.5 37 10982.0 38 11594.5 39 11823.0 40 11997.5 41 12226.0 42 12633.5 43 13070.0 44 13066.5 45 13392.5 46 15478.5 47 27232.0 48 53556.0 49 51603.0 50 26229.5 51 15911.0 52 11286.5 53 9081.0 54 7948.5 55 7110.0 56 6177.0 57 4939.0 58 3873.5 59 3050.5 60 2325.0 61 1728.0 62 1248.5 63 901.5 64 597.5 65 378.0 66 242.0 67 149.5 68 83.5 69 47.5 70 28.0 71 22.0 72 11.0 73 4.0 74 3.0 75 1.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05865167790453551 2 0.003332481699121336 3 0.05109805271986048 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0022216544660808905 16-17 0.0 18-19 0.0 20-21 0.012441265010052988 22-23 0.0 24-25 0.0 26-27 0.005443053441898182 28-29 0.01477400219943792 30-31 0.010886106883796364 32-33 0.005443053441898182 34-35 0.006442797951634583 36-37 0.023993868233673617 38-39 0.05565244437532631 40-41 0.004887639825377959 42-43 0.012330182286748943 44-45 0.010775024160492318 46-47 0.0037768125923375137 48-49 7.775790631283117E-4 50-51 0.0011108272330404452 52-53 5.554136165202226E-4 54-55 7.775790631283117E-4 56-57 0.010441775990580184 58-59 0.0073314597380669385 60-61 0.004221143485553692 62-63 0.004887639825377959 64-65 0.08386745609455361 66-67 0.037545960476767046 68-69 0.02677093631627473 70-71 0.017217822112126902 72-73 0.0 74-75 0.0 76-77 3.332481699121336E-4 78-79 1.1108272330404453E-4 80-81 8.886617864323562E-4 82-83 5.554136165202226E-4 84-85 4.443308932161781E-4 86-87 0.004443308932161781 88-89 0.0 90-91 7.775790631283117E-4 92-93 3.332481699121336E-4 94-95 0.0024438199126889794 96-97 0.00588738433511436 98-99 0.007775790631283116 100-101 0.010108527820668052 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 450115.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.85234126291993 #Duplication Level Percentage of deduplicated Percentage of total 1 89.01459844582224 53.27732123561134 2 7.1811592160743345 8.59618384127687 3 1.596555545206736 2.8667276201076217 4 0.6763804161874115 1.6193180597281907 5 0.37655288628793304 1.1268785926821425 6 0.24099709209868597 0.8654544119797137 7 0.14576236893121178 0.6106953333993765 8 0.10502991860242349 0.5029029224807161 9 0.09369413784063806 0.5047032161135635 >10 0.49623154122865876 5.28406056352119 >50 0.03330249736545347 1.3934506765492094 >100 0.02611218543277918 3.5296069394875738 >500 0.0045412496404833354 1.8435135006410424 >1k 0.008325624340886115 11.480565830383568 >5k 3.78437470040278E-4 1.5764656548054616 >10k+ 3.78437470040278E-4 4.9221516012323985 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 21731 4.827877320240383 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 6960 1.5462715083922998 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 4276 0.9499794496961887 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3744 0.8317874321006854 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 3713 0.8249003032558346 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3472 0.7713584306232851 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3280 0.7287026648745321 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3270 0.7264810104084511 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2992 0.6647190162514024 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2771 0.6156204525510147 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2743 0.6093998200459882 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2720 0.6042900147740022 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2458 0.5460826677626829 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1739 0.38634571165146686 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1671 0.3712384612821168 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1531 0.34013529875698434 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1512 0.3359141552714306 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1391 0.30903213623185183 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1375 0.30547748908612243 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1302 0.28925941148373197 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 1298 0.2883707496972996 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1234 0.2741521611143819 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 1177 0.26148873065772077 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1017 0.22594225920042654 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 838 0.18617464425757863 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 812 0.18039834264576832 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 800 0.17773235728647124 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 758 0.1684014085289315 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 723 0.16062561789764837 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 720 0.1599591215578241 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 648 0.14396320940204171 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 598 0.13285493707163726 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 587 0.13041111715894826 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 571 0.12685647001321884 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 559 0.12419048465392177 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 525 0.11663685946924675 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 480 0.10663941437188275 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 456 0.10130744365328861 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.2216544660808905E-4 0.0 10-11 0.0 0.0 0.0 5.554136165202226E-4 0.0 12-13 0.0 0.0 0.0 6.664963398242672E-4 0.0 14-15 0.0 0.0 0.0 0.0013329926796485344 0.0 16-17 0.0 0.0 0.0 0.002110571742776846 0.0 18-19 0.0 0.0 0.0 0.0031103162525132465 0.0 20-21 0.0 0.0 0.0 0.005443053441898181 0.0 22-23 0.0 0.0 0.0 0.008886617864323562 0.0 24-25 0.0 0.0 0.0 0.013107761349877255 0.0 26-27 0.0 0.0 0.0 0.018550814791775436 0.0 28-29 0.0 0.0 0.0 0.03276940337469313 0.0 30-31 0.0 0.0 0.0 0.07075969474467636 0.0 32-33 0.0 0.0 0.0 0.1459626984215145 0.0 34-35 0.0 0.0 0.0 0.19606100663163856 0.0 36-37 0.0 0.0 0.0 0.2110571742776846 0.0 38-39 0.0 0.0 0.0 0.22483143196738611 0.0 40-41 0.0 0.0 0.0 0.2477144729680193 0.0 42-43 0.0 0.0 0.0 0.27204158937160505 0.0 44-45 0.0 0.0 0.0 0.29859036024127167 0.0 46-47 0.0 0.0 0.0 0.32369505570798573 0.0 48-49 0.0 0.0 0.0 0.3505770747475645 0.0 50-51 0.0 2.2216544660808905E-4 0.0 0.38301322995234555 0.0 52-53 0.0 2.2216544660808905E-4 0.0 0.46599202426046676 0.0 54-55 0.0 2.2216544660808905E-4 0.0 0.5546360374570943 0.0 56-57 0.0 2.2216544660808905E-4 0.0 0.6671628361640913 0.0 58-59 0.0 2.2216544660808905E-4 0.0 0.8454506070670829 0.0 60-61 0.0 2.2216544660808905E-4 0.0 1.0760583406462794 0.0 62-63 0.0 2.2216544660808905E-4 0.0 1.175255212556791 0.0 64-65 0.0 2.2216544660808905E-4 0.0 1.2911144929629095 0.0 66-67 0.0 2.2216544660808905E-4 0.0 1.949168545816069 0.0 68-69 0.0 2.2216544660808905E-4 0.0 2.532797174055519 0.0 70-71 0.0 2.2216544660808905E-4 0.0 2.8830410006331713 0.0 72-73 0.0 2.2216544660808905E-4 0.0 3.068326983104318 0.0 74-75 0.0 2.2216544660808905E-4 0.0 4.072403719049576 0.0 76-77 0.0 2.2216544660808905E-4 0.0 4.261022183219843 0.0 78-79 0.0 2.2216544660808905E-4 0.0 4.3911000522088806 0.0 80-81 0.0 2.2216544660808905E-4 0.0 4.651477955633561 0.0 82-83 0.0 2.2216544660808905E-4 0.0 5.255656887684259 0.0 84-85 0.0 2.2216544660808905E-4 0.0 5.811737000544305 0.0 86-87 0.0 2.2216544660808905E-4 0.0 7.491085611454849 0.0 88-89 0.0 2.2216544660808905E-4 0.0 9.902691534385657 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTAACG 15 6.608336E-4 95.02512 2 TACCTGG 600 0.0 58.598827 2 ATAACGC 130 0.0 58.464005 3 TATAACG 145 0.0 52.427654 2 ACCTGGG 665 0.0 51.43074 3 CCTGGGG 410 0.0 47.480892 4 GTATAAC 170 0.0 44.742573 1 GTCTACG 45 9.580058E-4 42.256874 1 CTCCGTA 45 9.63789E-4 42.20524 7 TAACGCA 170 0.0 41.8949 4 GTACCTG 855 0.0 41.700863 1 TCACGCA 100 3.034438E-8 37.984715 4 TATCACG 105 4.6358764E-8 36.200047 2 CCCTATA 140 4.5292836E-10 33.937546 2 TAGGACG 65 0.0058518373 29.21901 4 CTATCGT 35 0.008334459 27.180248 64-65 GTACCCG 70 0.00835274 27.165134 1 CAGCGTA 35 0.008402547 27.134953 90-91 CACGCAG 140 5.813308E-7 27.131939 5 AGTACGC 230 5.456968E-12 26.854925 2 >>END_MODULE