##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003841_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 300600 Sequences flagged as poor quality 0 Sequence length 121 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.728499667332002 31.0 31.0 34.0 27.0 34.0 2 30.41843978709248 31.0 31.0 34.0 26.0 34.0 3 30.590302727877578 31.0 31.0 34.0 26.0 34.0 4 33.9208250166334 37.0 35.0 37.0 30.0 37.0 5 33.80576513639388 37.0 35.0 37.0 28.0 37.0 6 33.91804391217565 37.0 35.0 37.0 29.0 37.0 7 33.8671124417831 37.0 35.0 37.0 29.0 37.0 8 33.97791749833666 37.0 35.0 37.0 30.0 37.0 9 35.37590818363273 39.0 35.0 39.0 30.0 39.0 10-11 35.26178476380572 39.0 35.0 39.0 28.5 39.0 12-13 35.245696939454426 39.0 35.0 39.0 29.0 39.0 14-15 36.10664504324684 39.0 36.0 41.0 27.0 41.0 16-17 36.14826846307385 39.0 36.0 41.0 27.0 41.0 18-19 36.11612109115103 39.0 36.0 41.0 27.0 41.0 20-21 36.074514304723884 39.0 36.0 41.0 27.0 41.0 22-23 35.89202262142382 39.0 36.0 41.0 27.0 41.0 24-25 35.70093313373253 39.0 35.5 41.0 27.0 41.0 26-27 35.50535096473719 39.0 35.0 41.0 26.0 41.0 28-29 35.41957418496341 39.0 35.0 40.0 26.0 41.0 30-31 35.086862940785096 38.0 35.0 40.0 25.0 41.0 32-33 34.80310379241517 38.0 34.0 40.0 24.5 41.0 34-35 34.49796739853626 38.0 34.0 40.0 23.5 41.0 36-37 34.484148369926814 38.0 34.0 40.0 24.0 41.0 38-39 34.29588157019295 38.0 33.5 40.0 23.0 41.0 40-41 34.518125415835 38.0 34.0 40.0 23.0 41.0 42-43 34.321146041250834 38.0 34.0 40.0 21.5 41.0 44-45 33.98559381237525 38.0 33.0 40.0 17.0 41.0 46-47 33.51207085828344 38.0 33.0 40.0 14.0 41.0 48-49 33.030224550898204 38.0 33.0 40.0 9.0 41.0 50-51 32.1581370592149 37.5 31.0 39.5 7.0 40.5 52-53 32.23744344644045 37.5 31.5 39.5 3.5 40.5 54-55 32.56447771124418 38.0 32.0 40.0 2.0 41.0 56-57 32.00346640053227 37.5 31.0 40.0 2.0 41.0 58-59 31.619602461743177 37.0 31.0 40.0 2.0 41.0 60-61 30.936957751164336 36.0 29.5 39.5 2.0 41.0 62-63 30.79003825681969 36.0 29.5 39.0 2.0 41.0 64-65 30.402115768463073 35.5 29.0 39.0 2.0 40.5 66-67 29.837937458416498 35.0 28.0 39.0 2.0 40.0 68-69 29.456796407185628 35.0 28.0 38.0 2.0 39.0 70-71 28.68995508982036 34.0 26.5 37.0 2.0 39.0 72-73 28.24563373253493 34.0 26.0 36.5 2.0 38.0 74-75 27.834590818363274 34.0 26.0 36.0 2.0 37.0 76-77 27.353153692614768 34.0 26.0 35.5 2.0 36.5 78-79 26.616472055888224 33.5 23.5 35.0 2.0 36.0 80-81 26.165340984697274 33.0 21.0 35.0 2.0 36.0 82-83 25.65228210246174 32.0 20.5 35.0 2.0 35.5 84-85 25.35624916833001 32.0 20.0 35.0 2.0 35.0 86-87 25.393256819693946 32.0 20.0 35.0 2.0 35.0 88-89 24.966395542248836 32.0 18.0 35.0 2.0 35.0 90-91 24.58030272787758 32.0 16.0 35.0 2.0 35.0 92-93 24.180414171656686 31.0 9.0 34.0 2.0 35.0 94-95 23.793364936793083 31.0 7.0 34.0 2.0 35.0 96-97 23.443170326014638 31.0 3.5 34.0 2.0 35.0 98-99 22.980853293413176 31.0 2.0 34.0 2.0 35.0 100-101 22.504973386560213 30.5 2.0 34.0 2.0 35.0 102-103 21.833933799068532 29.0 2.0 34.0 2.0 35.0 104-105 21.100953093812375 29.0 2.0 34.0 2.0 35.0 106-107 20.236061210911508 26.0 2.0 34.0 2.0 35.0 108-109 19.919163339986692 27.0 2.0 34.0 2.0 35.0 110-111 19.445339321357284 26.0 2.0 34.0 2.0 35.0 112-113 18.512777777777778 24.5 2.0 33.0 2.0 35.0 114-115 17.592267132401865 21.0 2.0 33.0 2.0 35.0 116-117 16.512875914836993 14.0 2.0 32.0 2.0 34.0 118-119 15.518815701929475 2.0 2.0 32.0 2.0 34.0 120-121 13.998474717232202 2.0 2.0 30.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7731949676666581 1101 2 0.9261426707080247 1101 3 0.7307809553687719 1101 4 0.838933617723626 1101 5 1.195127226297366 1101 6 0.8942628783672433 1101 7 1.087262559669682 1101 8 0.959220004753 1101 9 1.048401817800304 1101 10-11 0.8969918188553123 1101 12-13 0.8277303318842613 1101 14-15 0.9896589561720361 1101 16-17 1.1310909760949173 1101 18-19 1.2588598139354303 1101 20-21 1.2336734260146116 1101 22-23 1.03450414326867 1101 24-25 0.7183048405468284 1101 26-27 1.1159280794229076 1101 28-29 1.0581887174076599 1101 30-31 1.2511436218623402 1101 32-33 1.4922286611180127 1101 34-35 1.4463773581308885 1101 36-37 1.514025514043574 1101 38-39 1.4254960688418095 1101 40-41 1.578409378190912 1101 42-43 1.4087155867109828 1101 44-45 1.5751870069472105 1101 46-47 1.5107058877378847 1101 48-49 1.971526448860054 1101 50-51 1.863385631749459 1101 52-53 1.8794940000983154 1101 54-55 2.101901257305741 1101 56-57 1.8602839219140321 1101 58-59 1.715524487796344 1101 60-61 1.568365548961161 1101 62-63 1.5383189579918302 1101 64-65 1.704006470594397 1101 66-67 1.987400817338191 1101 68-69 2.2471408397384494 1101 70-71 1.3972283996639092 1101 72-73 1.3986598320027461 1101 74-75 1.3164762946622126 1101 76-77 1.6995312652498988 1101 78-79 1.7484651527769728 1101 80-81 1.5543783071377497 1101 82-83 1.4770532634561597 1101 84-85 1.2213836873499844 1101 86-87 1.5987362948503652 1101 88-89 1.3393228944975881 1101 90-91 1.4685857579298869 1101 92-93 1.6645000529546614 1101 94-95 1.6466748244392129 1101 96-97 1.6532993492846053 1101 98-99 1.801731233918428 1101 100-101 2.4078014906820187 1101 102-103 1.9361871642687234 1101 104-105 1.547790575227502 1101 106-107 1.492436297152473 1101 108-109 1.489053090139059 1101 110-111 1.3062339128711322 1101 112-113 1.1889606950029794 1101 114-115 1.5450188786389543 1101 116-117 1.4967369190711377 1101 118-119 1.6151910775073866 1101 120-121 1.9358705831706704 1102 1 1.2102607112641195 1102 2 1.640762531745974 1102 3 1.4442469799003383 1102 4 1.5384219163164516 1102 5 1.745099634554819 1102 6 1.8351914659208646 1102 7 1.3963715972195345 1102 8 1.6109683178941978 1102 9 1.749163099060489 1102 10-11 1.783928382930867 1102 12-13 1.6439066180357145 1102 14-15 1.9987454186598086 1102 16-17 1.8371197718407757 1102 18-19 2.0027300574952704 1102 20-21 2.2993010127404787 1102 22-23 2.188980276411371 1102 24-25 2.115356286604964 1102 26-27 1.9699897093423857 1102 28-29 1.9302508239117842 1102 30-31 1.8799155384851076 1102 32-33 1.9620533281358448 1102 34-35 2.12522201662037 1102 36-37 2.0008943529328818 1102 38-39 2.1029454630776385 1102 40-41 2.6190319482363194 1102 42-43 2.7511197054056424 1102 44-45 3.1630353526968307 1102 46-47 3.0719689624613338 1102 48-49 2.6974024866858315 1102 50-51 2.731841999171138 1102 52-53 2.805886756011727 1102 54-55 3.127539629894599 1102 56-57 3.063688366692922 1102 58-59 2.92270398462329 1102 60-61 2.877314679821268 1102 62-63 2.798295881261698 1102 64-65 2.9106654868233583 1102 66-67 3.1238545259192243 1102 68-69 2.863063783526549 1102 70-71 2.864080683507691 1102 72-73 2.9184569072780384 1102 74-75 2.9078519938620495 1102 76-77 3.337944614636612 1102 78-79 3.1428891124428624 1102 80-81 2.5668416226618724 1102 82-83 2.5100630208996613 1102 84-85 2.5621479786184516 1102 86-87 2.5916032023176925 1102 88-89 2.8421886732118153 1102 90-91 2.692392415064859 1102 92-93 2.0908112878105563 1102 94-95 2.16779818461179 1102 96-97 2.533880406299179 1102 98-99 2.400459505421175 1102 100-101 2.505432363333586 1102 102-103 2.2338456286628343 1102 104-105 2.2865908360948843 1102 106-107 1.8424939889777896 1102 108-109 1.959633394651565 1102 110-111 2.0452661952099085 1102 112-113 1.4296767007219948 1102 114-115 1.8327286385907957 1102 116-117 1.2968002546619797 1102 118-119 1.5018674012839437 1102 120-121 1.2073952777802468 1103 1 0.8597980252221085 1103 2 1.2478752301028244 1103 3 1.2562785762551485 1103 4 1.1503110124073714 1103 5 1.3860957289920322 1103 6 1.3135349443347124 1103 7 1.454119389345074 1103 8 1.1407966138725243 1103 9 1.5828512170014548 1103 10-11 1.231537545170056 1103 12-13 1.1436251360329663 1103 14-15 1.50019681023646 1103 16-17 1.3003775741700423 1103 18-19 1.3503207474780226 1103 20-21 1.531149821719751 1103 22-23 1.4851697137992161 1103 24-25 1.0878854023996283 1103 26-27 1.2904877565609993 1103 28-29 1.0898687120930575 1103 30-31 1.4411114851442761 1103 32-33 1.7881854135656354 1103 34-35 2.038560745025414 1103 36-37 1.765962436392293 1103 38-39 1.7021458782636003 1103 40-41 2.2610572112460616 1103 42-43 2.4398226011517465 1103 44-45 2.4436465204952853 1103 46-47 2.5233737335812805 1103 48-49 2.371587068509129 1103 50-51 2.037804139841768 1103 52-53 2.646145719454257 1103 54-55 2.6967710081008534 1103 56-57 2.680015202483361 1103 58-59 2.6980153740888255 1103 60-61 2.5566319100383623 1103 62-63 2.4360461363547685 1103 64-65 2.8395868829740927 1103 66-67 2.5444414158949478 1103 68-69 2.3443980082195566 1103 70-71 2.607773727383698 1103 72-73 2.341216486462443 1103 74-75 1.985202368584968 1103 76-77 2.249704404932519 1103 78-79 2.5243675966622803 1103 80-81 2.2070152618723107 1103 82-83 2.409063759789916 1103 84-85 2.33906469513089 1103 86-87 2.5640751420805437 1103 88-89 2.0341154175205993 1103 90-91 1.63667657114663 1103 92-93 1.6095562299308206 1103 94-95 1.179114640753376 1103 96-97 1.2703824646363238 1103 98-99 1.513509714773413 1103 100-101 2.007657830417781 1103 102-103 1.9303992333917321 1103 104-105 1.7237411826696025 1103 106-107 2.0313948017570738 1103 108-109 1.7568300098304803 1103 110-111 1.4448826759811695 1103 112-113 1.2048297565609865 1103 114-115 0.9819536834519891 1103 116-117 0.7024202187730229 1103 118-119 1.098807164135355 1103 120-121 1.1409435343510932 1104 1 1.2822170905025452 1104 2 1.3334953843769277 1104 3 1.4523960770935957 1104 4 2.0082091757169636 1104 5 2.034697989837582 1104 6 1.8518174119312292 1104 7 1.9900936638425009 1104 8 1.540784776984836 1104 9 1.338952146197137 1104 10-11 1.8774567781397309 1104 12-13 1.6460221057897257 1104 14-15 1.7149870014021928 1104 16-17 1.6675381020952287 1104 18-19 1.8738268336111048 1104 20-21 2.1376226264702822 1104 22-23 1.9506750502627526 1104 24-25 1.7274324441641156 1104 26-27 2.201494089295906 1104 28-29 2.3186067025840984 1104 30-31 2.293821146017436 1104 32-33 2.1999414158540986 1104 34-35 2.047842837787165 1104 36-37 1.9099581751127275 1104 38-39 2.3464276751031505 1104 40-41 2.6005884704936335 1104 42-43 2.1869176316317294 1104 44-45 2.3310974467317465 1104 46-47 2.1272621511866845 1104 48-49 2.237120024377269 1104 50-51 2.421625087187337 1104 52-53 2.1870618945612783 1104 54-55 2.0419741093654267 1104 56-57 1.7374339718415968 1104 58-59 2.0191895933474484 1104 60-61 2.5688929528681683 1104 62-63 2.3052978477714454 1104 64-65 2.3137627396688387 1104 66-67 2.4696026934896906 1104 68-69 2.329394753075441 1104 70-71 2.5044179697242406 1104 72-73 1.8563479745565097 1104 74-75 2.305794607532274 1104 76-77 1.7680515808483968 1104 78-79 1.4341113280438407 1104 80-81 1.3663693623674895 1104 82-83 1.4867926556055764 1104 84-85 0.9519787395981325 1104 86-87 1.7932076954944982 1104 88-89 1.6205838820789857 1104 90-91 1.8464952694835475 1104 92-93 1.5558759442672603 1104 94-95 1.7190838648453877 1104 96-97 1.4679203919972892 1104 98-99 1.6935290483566376 1104 100-101 1.5055962391455147 1104 102-103 1.0585729989029886 1104 104-105 1.081284239348566 1104 106-107 0.9637173964028563 1104 108-109 1.6640386744322662 1104 110-111 2.6020864085464233 1104 112-113 2.4466080516545965 1104 114-115 2.1960040691372438 1104 116-117 1.912682800398228 1104 118-119 1.2371409249118557 1104 120-121 0.7261888538484182 1105 1 0.9704183825800676 1105 2 1.2434871267386107 1105 3 1.1096069383166949 1105 4 0.9427795356528463 1105 5 1.1982963771693917 1105 6 1.6057883644741011 1105 7 1.3889861688213685 1105 8 0.8684187207357468 1105 9 1.2027496883353805 1105 10-11 1.785141239207391 1105 12-13 1.2035098407681062 1105 14-15 2.386794452706418 1105 16-17 1.3545978971115034 1105 18-19 1.1605854677005851 1105 20-21 1.3686179147590138 1105 22-23 1.8244303327225566 1105 24-25 1.6402414984216591 1105 26-27 1.4150754756076651 1105 28-29 1.70292690465336 1105 30-31 1.0415502954806897 1105 32-33 0.6731769528434626 1105 34-35 1.6478331229185557 1105 36-37 1.1632005125101088 1105 38-39 1.1804491449627719 1105 40-41 0.9026237354666762 1105 42-43 1.609854436411446 1105 44-45 1.2925294183643317 1105 46-47 1.4532931416175359 1105 48-49 1.1040991859841043 1105 50-51 0.4429006494353658 1105 52-53 1.193740866728188 1105 54-55 0.5572264530774795 1105 56-57 0.2983219108322288 1105 58-59 0.15655783544196566 1105 60-61 -0.4047068916939338 1105 62-63 -0.6053253610496547 1105 64-65 -0.6278751871782013 1105 66-67 -1.0017627312929385 1105 68-69 -0.8693714586115462 1105 70-71 -1.4979135987412953 1105 72-73 -1.5978949943073388 1105 74-75 -0.9383643277181299 1105 76-77 -1.4847293002901836 1105 78-79 -1.4200927813455806 1105 80-81 -2.174505947293948 1105 82-83 -2.0867488997448866 1105 84-85 -1.7235446489480388 1105 86-87 -1.288421487970794 1105 88-89 -1.8378121931140967 1105 90-91 -1.9006509878633224 1105 92-93 -2.0107437672011415 1105 94-95 -1.9080594780409044 1105 96-97 -1.3884209884855387 1105 98-99 -0.4830629757362388 1105 100-101 -0.3418560365624579 1105 102-103 -1.2956311104864326 1105 104-105 -0.9030262478520967 1105 106-107 -1.3480327371765881 1105 108-109 -0.29780617911608687 1105 110-111 -1.3541966827247691 1105 112-113 -2.197900618865635 1105 114-115 -1.012209852269466 1105 116-117 -2.3644338265993934 1105 118-119 -2.0784951561101472 1105 120-121 -1.7352431177841634 1106 1 0.31159485316830526 1106 2 0.9342434292596202 1106 3 0.6759934929385452 1106 4 0.5713509642242727 1106 5 1.114262763724014 1106 6 0.6595698770791429 1106 7 0.3402466730230529 1106 8 0.14404897283658613 1106 9 0.7035900244698325 1106 10-11 -0.05603523138084654 1106 12-13 0.4967871516924731 1106 14-15 0.7010801669921349 1106 16-17 0.7999760483720024 1106 18-19 1.126972022322434 1106 20-21 0.7173574105573337 1106 22-23 0.6924975596133081 1106 24-25 1.286459985816613 1106 26-27 0.5192771562799265 1106 28-29 0.5701537954096665 1106 30-31 0.4995334887580043 1106 32-33 0.1580509024232981 1106 34-35 0.7352280809017486 1106 36-37 0.7951332856193574 1106 38-39 0.6762474642905048 1106 40-41 0.7463212144582698 1106 42-43 0.24682922632741366 1106 44-45 -0.549487388358358 1106 46-47 0.01967969623937904 1106 48-49 -0.8345562762007681 1106 50-51 -0.846174980816734 1106 52-53 -0.6785280408348449 1106 54-55 -0.9234458158300853 1106 56-57 -0.7436948958904637 1106 58-59 -1.0669715763227394 1106 60-61 -0.9240346227863725 1106 62-63 -0.744821159368982 1106 64-65 -0.21779115356475387 1106 66-67 -0.9219307985198313 1106 68-69 -0.5155899460065001 1106 70-71 -0.7348883886572608 1106 72-73 -1.1987353304417958 1106 74-75 -2.031641638642501 1106 76-77 -2.6561578717187544 1106 78-79 -2.3293364788245725 1106 80-81 -2.383749644772937 1106 82-83 -2.3833875552070722 1106 84-85 -2.315141287603499 1106 86-87 -2.240522328306927 1106 88-89 -2.5361315208451884 1106 90-91 -2.5199787189557554 1106 92-93 -2.429231162159123 1106 94-95 -2.4088998141753564 1106 96-97 -2.7354798120149475 1106 98-99 -2.402390706828676 1106 100-101 -2.284713179419601 1106 102-103 -2.634286572671307 1106 104-105 -2.745883390709242 1106 106-107 -3.0791251741513754 1106 108-109 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0.8564588636426684 2316 64-65 1.1173016121478874 2316 66-67 1.1267532227659487 2316 68-69 0.9213149871158919 2316 70-71 1.2301482861593662 2316 72-73 1.4068306123167282 2316 74-75 1.5469862917472632 2316 76-77 1.0576059712267387 2316 78-79 0.9084601557216665 2316 80-81 1.0348897914416462 2316 82-83 1.071250730425028 2316 84-85 0.9094811775194707 2316 86-87 0.8296775853993843 2316 88-89 1.0826229736176352 2316 90-91 1.2433464644183232 2316 92-93 1.884316946570923 2316 94-95 1.800750696395621 2316 96-97 1.4022449111261395 2316 98-99 1.6933665202675456 2316 100-101 1.5126980099950416 2316 102-103 1.7285208485857027 2316 104-105 1.9088843354521323 2316 106-107 2.051609760409246 2316 108-109 1.7087480318103374 2316 110-111 2.0921368629184265 2316 112-113 1.7483096500830193 2316 114-115 2.240805290217848 2316 116-117 1.4965614272478671 2316 118-119 1.1352358130501319 2316 120-121 1.0668053299567894 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7365.0 3 1234.0 4 950.0 5 1013.0 6 1133.0 7 1142.0 8 1362.0 9 1578.0 10 1827.0 11 2326.0 12 2818.0 13 3475.0 14 4358.0 15 4587.0 16 3734.0 17 3738.0 18 3687.0 19 4063.0 20 4326.0 21 4359.0 22 4633.0 23 5245.0 24 5646.0 25 6280.0 26 7105.0 27 8226.0 28 9135.0 29 10653.0 30 12457.0 31 14837.0 32 17457.0 33 21307.0 34 26154.0 35 31170.0 36 34054.0 37 22398.0 38 4764.0 39 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.68043558767303 17.23066862841262 15.199861209810065 19.889034574104286 2 13.344002052715629 21.265188576367436 32.67767975610497 32.71312961481196 3 29.164614855338872 21.485331516496565 32.350937312385746 16.999116315778824 4 9.845786448635959 12.750951185946732 47.21491405596481 30.188348309452493 5 9.839283606253266 37.82788336678078 39.47518424203585 12.857648784930099 6 25.31661793027154 33.633509054176656 18.37547598088319 22.67439703466861 7 18.017076263184872 35.41638565917613 24.136070571097143 22.430467506541856 8 19.496672928463745 21.782622332693663 24.721507418037348 33.999197320805244 9 16.45413798569352 22.248565810818558 33.11366602700087 28.183630176487057 10-11 24.89486836203728 18.286329008913004 27.132561974552576 29.686240654497137 12-13 31.401154350058423 20.343944383461398 25.26350577765188 22.9913954888283 14-15 26.070029069179252 20.547728085143692 34.71692959323383 18.665313252443227 16-17 18.838086951242953 25.99734917085707 33.11782699461403 22.04673688328595 18-19 15.614982099524283 33.48763324176519 30.520521887821438 20.376862770889087 20-21 19.26521030850644 25.63062197083911 23.982690991018337 31.12147672963611 22-23 16.39824971123608 25.230631739581312 29.211948503064693 29.159170046117914 24-25 25.3667288912722 23.191228522814846 26.170083961059877 25.27195862485308 26-27 22.655039184569695 18.87031737224751 36.044070203368506 22.43057323981429 28-29 17.783010037054968 29.709581499840613 31.731328733367853 20.77607972973657 30-31 17.650749425302863 25.849143477798897 29.27320840381771 27.226898693080525 32-33 19.44777546714431 24.661880113541493 31.166060336205604 24.724284083108593 34-35 25.503507390059895 22.926305172451595 27.074791150481285 24.495396287007225 36-37 21.957895944086136 20.242295652643225 36.1905308038967 21.60927759937394 38-39 23.068866416153384 23.64196614697529 31.794554371951506 21.49461306491982 40-41 19.462851803565133 28.34330394714872 32.68275570112783 19.511088548158316 42-43 17.939507390824076 28.87303635121803 25.222736586476437 27.964719671481454 44-45 18.43608297316576 24.903922524202756 26.32902256450555 30.330971938125938 46-47 23.81429424324297 26.716115344268708 27.8862208190401 21.583369593448218 48-49 23.0562704272478 23.292712131381986 28.595954519544385 25.055062921825826 50-51 15.444724304312201 25.418862555086875 37.21832778671306 21.91808535388786 52-53 19.094286673176523 29.455415070824202 26.38549482500122 25.06480343099805 54-55 15.758059238820621 22.804517288952002 32.65437949679722 28.783043975430157 56-57 22.929903029771918 20.38228582792082 27.709655565255197 28.978155577052068 58-59 25.49038767708795 22.00043466467642 27.667754451522253 24.84142320671338 60-61 22.45710701664464 20.769544632981738 35.3546764142545 21.41867193611913 62-63 19.84855922096893 23.90581591292071 32.58996118676403 23.655663679346333 64-65 16.74535661813698 29.47153751562413 31.510054264860425 22.273051601378466 66-67 18.450278489040926 23.185266900793128 26.371950705246928 31.99250390491902 68-69 15.723501682365937 24.44481924053195 31.101759948727896 28.72991912837422 70-71 22.381887903214917 22.67185158180419 26.577922253277702 28.36833826170319 72-73 19.616342169447467 18.72446008557612 35.28082301609405 26.378374728882363 74-75 17.499417676309047 26.159499302899462 32.19777400439528 24.14330901639621 76-77 17.260598613183532 23.369275331006218 30.167646800667075 29.20247925514317 78-79 17.80759578158209 21.266031016192404 32.95870743953764 27.967665762687865 80-81 22.490751118714343 21.631679630550384 28.902522268946512 26.975046981788765 82-83 19.02153559631975 20.365359505791297 35.423576130340464 25.18952876754849 84-85 20.13419083231712 21.270832384585933 30.52369169841808 28.071285084678866 86-87 19.05313628368134 25.318789031734706 31.948723916302807 23.679350768281147 88-89 17.991580282478825 23.218821576875463 27.095905844889646 31.693692295756072 90-91 16.752239108067535 21.052338623981647 29.66350127346636 32.53192099448446 92-93 22.32884106127819 23.568910553747028 30.224838497478366 23.877409887496416 94-95 19.63421030883323 22.576145749179048 28.672190842102246 29.11745309988548 96-97 13.44472331295794 24.00551889783967 34.455666653173026 28.094091136029363 98-99 19.345843581412765 25.734234826938195 25.799181503186603 29.120740088462433 100-101 17.170742568746057 20.674590188821828 31.014246821201212 31.140420421230903 102-103 21.852474001814727 19.21285286865477 28.48938990700357 30.445283222526925 104-105 25.814388828926067 19.87655153804641 27.776578521316786 26.532481111710737 106-107 23.868829789028112 19.848558949019342 32.103816381941755 24.17879488001079 108-109 20.642073415475735 21.61364962543469 30.755336838945347 26.98894012014423 110-111 19.67634210925773 24.7835253242482 28.9124825015601 26.627650064933974 112-113 22.69107394027706 20.592034917647375 24.417561363920004 32.29932977815555 114-115 20.278716615058727 20.901553635155466 29.877962397037095 28.941767352748716 116-117 26.24079618774512 19.89794627419559 26.663686585417285 27.197570952642007 118-119 21.9071360743748 18.68359844760149 33.57154543261892 25.837720045404787 120-121 20.228389669001945 25.131633537628133 30.19813335042349 24.441843442946432 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 961.0 1 1174.0 2 829.0 3 243.0 4 221.5 5 242.0 6 218.0 7 166.5 8 143.5 9 129.0 10 107.5 11 104.0 12 116.0 13 116.0 14 130.5 15 150.5 16 176.0 17 209.0 18 244.5 19 287.0 20 314.0 21 360.5 22 427.5 23 477.0 24 571.5 25 691.0 26 804.5 27 910.5 28 1050.0 29 1387.5 30 1825.0 31 2112.0 32 2496.0 33 3054.0 34 3537.5 35 3780.5 36 4061.5 37 4487.5 38 4506.0 39 4182.0 40 3893.0 41 3916.5 42 4015.0 43 4268.5 44 5145.5 45 6827.5 46 13110.5 47 28974.0 48 56977.0 49 58225.5 50 29202.0 51 12206.0 52 6758.5 53 4784.0 54 3884.0 55 3090.0 56 2370.0 57 1685.0 58 1139.0 59 921.0 60 695.0 61 505.0 62 410.5 63 340.0 64 226.0 65 125.0 66 83.5 67 66.5 68 50.0 69 44.5 70 40.5 71 26.5 72 14.5 73 13.0 74 10.5 75 5.5 76 5.0 77 4.5 78 2.5 79 1.5 80 2.0 81 2.0 82 0.5 83 0.0 84 0.0 85 1.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2884231536926148 2 1.466067864271457 3 1.3685961410512308 4 1.373253493013972 5 1.3689288090485694 6 1.3662674650698603 7 1.3466400532268794 8 1.361610113107119 9 1.3609447771124419 10-11 1.352960745176314 12-13 1.3537924151696608 14-15 1.3526280771789754 16-17 1.3602794411177646 18-19 1.3637724550898205 20-21 1.3662674650698603 22-23 1.3564537591483699 24-25 1.3617764471057885 26-27 1.370093147039255 28-29 1.3800731869594145 30-31 1.3770791749833666 32-33 1.3787425149700598 34-35 1.3820691949434465 36-37 1.3785761809713906 38-39 1.3779108449767132 40-41 1.3790751829673986 42-43 1.369760479041916 44-45 1.362109115103127 46-47 1.374251497005988 48-49 1.3709248170326014 50-51 1.3383233532934131 52-53 1.372754491017964 54-55 1.3769128409846974 56-57 1.3010645375914838 58-59 1.2706254158349968 60-61 1.3466400532268794 62-63 1.3907185628742516 64-65 1.3917165668662674 66-67 1.3902195608782435 68-69 1.3780771789753825 70-71 1.4491017964071855 72-73 1.4545908183632734 74-75 1.4547571523619427 76-77 1.4577511643379906 78-79 1.3135395874916833 80-81 1.3115435795076513 82-83 1.399534264803726 84-85 1.3827345309381238 86-87 1.372754491017964 88-89 1.3815701929474384 90-91 1.3855622089155024 92-93 1.3855622089155024 94-95 1.3790751829673986 96-97 1.3852295409181636 98-99 1.3982035928143712 100-101 1.3915502328675982 102-103 1.37624750499002 104-105 1.3705921490352628 106-107 1.3689288090485694 108-109 1.371590153027279 110-111 1.3789088489687291 112-113 1.374251497005988 114-115 1.375249500998004 116-117 1.393047238855622 118-119 1.382401862940785 120-121 1.3749168330006654 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 300600.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.2814371257485 #Duplication Level Percentage of deduplicated Percentage of total 1 82.6688246749829 20.899866932801064 2 6.402968577293541 3.2375249500998002 3 2.7422495920837937 2.0798403193612773 4 1.5329754197589347 1.5502328675981372 5 1.113216485078162 1.407185628742515 6 0.7013527027738302 1.063872255489022 7 0.6079267329859467 1.0758483033932136 8 0.42370651086899314 0.856952761144378 9 0.361861150586873 0.8233532934131738 >10 2.922522238012527 14.676979374584164 >50 0.3000157903047529 5.16333998669328 >100 0.17106163482288542 8.409514304723885 >500 0.010526869835254487 1.8522954091816368 >1k 0.03289646823517027 16.404524284763806 >5k 0.003947576188220433 7.892215568862275 >10k+ 0.003947576188220433 12.60645375914837 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 15346 5.105123087159015 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 11898 3.95808383233533 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 10651 3.5432468396540253 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9886 3.2887558216899535 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 7736 2.573519627411843 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 6102 2.029940119760479 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 3390 1.127744510978044 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 3223 1.0721889554224884 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 3112 1.0352628077178976 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2953 0.9823685961410512 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2840 0.9447771124417831 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 2758 0.9174983366600133 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2618 0.8709248170326015 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2544 0.846307385229541 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2453 0.8160345974717231 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2428 0.8077178975382568 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1983 0.6596806387225549 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1739 0.5785096473719228 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1715 0.570525615435795 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1654 0.550232867598137 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1483 0.49334664005322687 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1476 0.4910179640718563 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1387 0.4614105123087159 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1286 0.4278110445775116 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1285 0.427478376580173 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 1222 0.4065202927478377 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1216 0.4045242847638057 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1198 0.39853626081170995 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1168 0.38855622089155023 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1136 0.37791084497671323 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 1045 0.3476380572188955 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 979 0.32568196939454425 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 833 0.27711244178310046 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 732 0.2435129740518962 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 700 0.2328675981370592 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 633 0.21057884231536925 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 619 0.20592149035262808 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 559 0.18596141051230872 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 513 0.17065868263473055 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 470 0.15635395874916833 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 461 0.15335994677312043 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 454 0.15103127079174983 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 437 0.1453759148369927 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 431 0.14337990685296076 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 422 0.14038589487691283 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 400 0.1330671989354624 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 375 0.124750499001996 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 374 0.12441783100465735 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 368 0.12242182302062543 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 363 0.12075848303393215 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 361 0.12009314703925482 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 342 0.11377245508982037 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 332 0.1104457751164338 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 324 0.10778443113772455 No Hit ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 320 0.10645375914836992 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 313 0.10412508316699934 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 306 0.10179640718562875 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 305 0.10146373918829008 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 303 0.10079840319361277 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.98003992015968E-4 0.0 10-11 0.0 0.0 0.0 0.002162341982701264 0.0 12-13 0.0 0.0 0.0 0.003825681969394544 0.0 14-15 0.0 0.0 0.0 0.0054890219560878245 0.0 16-17 0.0 0.0 0.0 0.00914836992681304 0.0 18-19 0.0 0.0 0.0 0.012974051896207584 0.0 20-21 0.0 0.0 0.0 0.01746506986027944 0.0 22-23 0.0 0.0 0.0 0.02594810379241517 0.0 24-25 0.0 0.0 0.0 0.035429141716566866 0.0 26-27 0.0 0.0 0.0 0.04557551563539587 0.0 28-29 0.0 0.0 0.0 0.07601463739188291 0.0 30-31 0.0 0.0 0.0 0.11909514304723887 0.0 32-33 0.0 0.0 0.0 0.20242847638057218 0.0 34-35 0.0 0.0 0.0 0.27694610778443113 0.0 36-37 0.0 0.0 0.0 0.3291749833666001 0.0 38-39 0.0 0.0 0.0 0.37907518296739856 0.0 40-41 0.0 0.0 0.0 0.4496007984031936 0.0 42-43 0.0 0.0 0.0 0.6132734530938124 0.0 44-45 0.0 0.0 0.0 0.841816367265469 0.0 46-47 0.0 0.0 0.0 1.089820359281437 0.0 48-49 0.0 0.0 0.0 1.5331004657351963 0.0 50-51 0.0 0.0 0.0 1.898037258815702 0.0 52-53 0.0 0.0 0.0 2.263639387890885 0.0 54-55 0.0 0.0 0.0 2.942781104457751 0.0 56-57 0.0 0.0 0.0 3.8987025948103793 0.0 58-59 0.0 0.0 0.0 4.782601463739189 0.0 60-61 0.0 0.0 0.0 5.621756487025948 0.0 62-63 0.0 0.0 0.0 7.379407850964737 0.0 64-65 0.0 0.0 0.0 8.11909514304724 0.0 66-67 0.0 0.0 0.0 9.678476380572189 0.0 68-69 0.0 0.0 0.0 11.409181636726547 0.0 70-71 0.0 0.0 0.0 12.198436460412509 0.0 72-73 0.0 0.0 0.0 13.639554224883566 0.0 74-75 0.0 0.0 0.0 15.96989354624085 0.0 76-77 0.0 0.0 0.0 16.76763140385895 0.0 78-79 0.0 0.0 0.0 17.74700598802395 0.0 80-81 0.0 0.0 0.0 19.566699933466403 0.0 82-83 0.0 0.0 0.0 21.55721889554225 0.0 84-85 0.0 0.0 0.0 23.41333998669328 0.0 86-87 0.0 0.0 0.0 25.57634730538922 0.0 88-89 0.0 0.0 0.0 26.89404524284764 0.0 90-91 0.0 0.0 0.0 27.496340652029275 0.0 92-93 0.0 0.0 0.0 28.23303393213573 0.0 94-95 0.0 0.0 0.0 28.875249500998002 0.0 96-97 0.0 0.0 0.0 29.566533599467732 0.0 98-99 0.0 0.0 0.0 29.842648037258815 0.0 100-101 0.0 0.0 0.0 29.89853626081171 0.0 102-103 0.0 0.0 0.0 30.036260811709916 0.0 104-105 0.0 0.0 0.0 30.17381902860945 0.0 106-107 0.0 0.0 0.0 30.417997338656022 0.0 108-109 0.0 0.0 0.0 30.998170326014638 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATAAG 20 9.609157E-4 86.473305 1 GTACTAG 85 0.0 67.822205 1 ATTAAGG 30 0.004846206 57.5027 3 TTAAGGT 30 0.0048526963 57.483273 4 ATAAGAC 65 6.920727E-7 53.079422 3 AGGGAGG 65 6.9288035E-7 53.07045 6 TAACGCC 45 3.7547262E-4 51.09624 4 TAAGACC 45 3.7547262E-4 51.09624 4 CCTATAA 35 0.008827098 49.41332 1 GTGTTCA 35 0.0089399675 49.254726 9 CGGGCGT 60 2.6818312E-5 47.91082 6 GAGGGAG 60 2.6818312E-5 47.91082 5 GCTTTTA 75 1.8239007E-6 46.1191 1 TATTAAG 50 6.239591E-4 46.095665 2 ATGACTC 75 1.8625778E-6 45.978848 8 CTAGGCA 190 0.0 45.38153 4 CCTGAGG 65 4.2475127E-5 44.322754 2 GGCGTCG 65 4.3116448E-5 44.21043 8 CCATGAC 80 2.8974064E-6 43.119743 6 TAGGCAT 190 0.0 42.363255 5 >>END_MODULE