##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003841_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 300600 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.702658017298734 31.0 31.0 34.0 30.0 34.0 2 31.804600798403193 31.0 31.0 34.0 30.0 34.0 3 31.914683965402528 31.0 31.0 34.0 30.0 34.0 4 35.50584497671324 37.0 35.0 37.0 33.0 37.0 5 35.44140385894877 37.0 35.0 37.0 33.0 37.0 6 35.62261809713905 37.0 35.0 37.0 33.0 37.0 7 35.61276779773786 37.0 35.0 37.0 33.0 37.0 8 35.673739188290085 37.0 35.0 37.0 33.0 37.0 9 37.219457751164335 39.0 37.0 39.0 33.0 39.0 10-11 37.12926147704591 39.0 37.0 39.0 33.0 39.0 12-13 37.13595974717232 39.0 37.0 39.0 33.0 39.0 14-15 37.90071856287425 39.0 37.0 41.0 33.0 41.0 16-17 38.08139554224884 39.5 37.0 41.0 33.0 41.0 18-19 38.258875582169 40.0 38.0 41.0 33.5 41.0 20-21 38.17468562874251 40.0 37.5 41.0 33.0 41.0 22-23 38.133494677312044 40.0 37.5 41.0 33.0 41.0 24-25 37.84295575515635 39.5 37.0 41.0 32.5 41.0 26-27 37.32660678642715 39.0 36.5 40.5 31.5 41.0 28-29 37.24494510978044 39.0 36.0 40.0 31.0 41.0 30-31 37.27768296739853 39.0 36.0 40.0 31.5 41.0 32-33 36.953351630073186 39.0 35.0 40.0 31.0 41.0 34-35 36.78623586161011 38.5 35.0 40.0 30.5 41.0 36-37 36.53487857618097 38.5 35.0 40.0 30.5 41.0 38-39 36.246919494344645 38.0 35.0 40.0 30.0 41.0 40-41 36.612879241516964 39.0 35.0 40.0 30.0 41.0 42-43 36.49004158349967 39.0 35.0 41.0 30.0 41.0 44-45 35.92013306719893 39.0 35.0 40.5 29.5 41.0 46-47 35.77680472388556 39.0 35.0 40.0 28.5 41.0 48-49 35.309233200266135 38.0 35.0 40.0 27.0 41.0 50-51 34.762910844976716 38.0 34.0 40.0 26.0 41.0 52-53 34.74883566200931 38.0 34.0 40.0 26.0 41.0 54-55 34.609491017964075 38.0 34.0 40.0 25.0 41.0 56-57 34.05466400532269 38.0 33.5 40.0 22.5 41.0 58-59 33.66335662009315 38.0 33.0 40.0 22.0 41.0 60-61 32.86230538922156 36.0 31.5 39.0 19.5 40.5 62-63 32.780608782435124 36.0 32.0 39.0 19.5 40.5 64-65 32.28663838988689 36.0 31.5 39.0 15.5 40.0 66-67 31.807796074517633 35.5 31.0 38.5 12.5 39.5 68-69 31.236581836327346 35.0 31.0 37.0 9.5 39.0 70-71 30.438892215568863 34.5 29.5 36.0 7.0 39.0 72-73 29.853055555555557 34.0 29.0 36.0 4.5 37.5 74-75 29.570610445775117 34.0 29.0 35.0 2.0 37.0 76-77 28.79142880904857 33.5 28.5 35.0 2.0 36.0 78-79 29.248819028609446 34.0 29.0 35.0 2.0 36.0 80-81 28.88824351297405 34.0 29.0 35.0 2.0 36.0 82-83 28.53613107119095 34.0 28.0 35.0 2.0 35.5 84-85 28.30679640718563 34.0 29.0 35.0 2.0 35.0 86-87 28.372677977378576 34.0 29.0 35.0 2.0 35.0 88-89 28.109279773785765 34.0 28.5 35.0 2.0 35.0 90-91 27.73685961410512 33.0 27.0 35.0 2.0 35.0 92-93 27.357995675316033 33.0 26.5 35.0 2.0 35.0 94-95 26.737539920159684 33.0 25.0 35.0 2.0 35.0 96-97 26.07781769793746 32.0 24.5 35.0 2.0 35.0 98-99 25.955192947438455 33.0 24.0 35.0 2.0 35.0 100-101 24.942245508982037 32.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.0710532661609129 1101 2 -0.008804881712631385 1101 3 0.048891241526046514 1101 4 -0.0366395045134027 1101 5 -0.0178591434780202 1101 6 0.06147490337227168 1101 7 0.25556239568370387 1101 8 -0.10000425605854701 1101 9 0.03652931133272119 1101 10-11 0.37606788650397505 1101 12-13 0.22048506419677238 1101 14-15 0.25721299637254447 1101 16-17 0.18965318939405762 1101 18-19 0.1259417575538322 1101 20-21 -0.005382608154278046 1101 22-23 0.07164603299741401 1101 24-25 0.14340597352528306 1101 26-27 0.16536632537969354 1101 28-29 0.2805422317370443 1101 30-31 0.5878561506994586 1101 32-33 0.7093155158063595 1101 34-35 0.4433270986204221 1101 36-37 0.4211591810255655 1101 38-39 0.44580002160098076 1101 40-41 0.23178851545620205 1101 42-43 0.3401031102956438 1101 44-45 0.4809420783945626 1101 46-47 0.48687187803526655 1101 48-49 0.8667763156608501 1101 50-51 0.35072309074831765 1101 52-53 0.7708310883396834 1101 54-55 0.9421491363116417 1101 56-57 1.0937392629147382 1101 58-59 0.8951053828497351 1101 60-61 0.22559815536293115 1101 62-63 0.13558240504330854 1101 64-65 0.48730319410384837 1101 66-67 0.36037351479651747 1101 68-69 0.24470095769925848 1101 70-71 0.4489874611651139 1101 72-73 -0.24027139074276604 1101 74-75 0.02061324613343274 1101 76-77 0.05376833244042345 1101 78-79 0.4793329625078435 1101 80-81 -0.052230622918184366 1101 82-83 0.45410798341836767 1101 84-85 0.4585135206955826 1101 86-87 0.2425640248213341 1101 88-89 -0.20923155464473808 1101 90-91 -0.01946681901289793 1101 92-93 -0.22235740113292124 1101 94-95 0.07310454064725747 1101 96-97 -0.24637923436300468 1101 98-99 0.38580053686932203 1101 100-101 0.19495612078004498 1102 1 0.07663780665620479 1102 2 0.128526745949344 1102 3 -0.011039277449544471 1102 4 -0.026160913197273317 1102 5 0.00175607713050141 1102 6 -0.13374294597681313 1102 7 0.07559525494073682 1102 8 0.15777159865838541 1102 9 0.3073297227005227 1102 10-11 0.09683945195911292 1102 12-13 0.11953064073877329 1102 14-15 0.23290717953154427 1102 16-17 0.28667265411424836 1102 18-19 0.16661449440385923 1102 20-21 0.2624767742923595 1102 22-23 0.23578241145578005 1102 24-25 0.28972373006578067 1102 26-27 0.3060340455492536 1102 28-29 0.10182926110970669 1102 30-31 0.582648209294284 1102 32-33 0.732696382187072 1102 34-35 0.5041606200792685 1102 36-37 0.3183642629228771 1102 38-39 0.45724745933143396 1102 40-41 0.6442348996837595 1102 42-43 1.0428303032120851 1102 44-45 1.1166620974579473 1102 46-47 1.3520642677310022 1102 48-49 1.673514971692157 1102 50-51 1.5066415195239955 1102 52-53 2.0191693129364694 1102 54-55 1.9321063691106914 1102 56-57 1.784529891755632 1102 58-59 1.6838122080435056 1102 60-61 1.269741808007975 1102 62-63 1.1969520842265986 1102 64-65 1.8750424270534047 1102 66-67 2.2989442625327463 1102 68-69 1.7796484079714148 1102 70-71 1.625135653905975 1102 72-73 1.701635449501044 1102 74-75 1.6947390895628338 1102 76-77 1.8136167032734427 1102 78-79 2.2703411898637995 1102 80-81 1.973461051900589 1102 82-83 1.933392228533375 1102 84-85 2.0599006328434797 1102 86-87 2.223165148557758 1102 88-89 2.3609768884284783 1102 90-91 2.251317915228455 1102 92-93 1.8540278297597972 1102 94-95 1.8546782733083518 1102 96-97 0.8243779081358014 1102 98-99 1.8885816591007405 1102 100-101 2.2328696833756787 1103 1 -0.17687192206551572 1103 2 0.06814885636065782 1103 3 -0.011927705020706725 1103 4 0.11868796615249977 1103 5 -0.062069966991799674 1103 6 0.04057358053527338 1103 7 0.35444716022204403 1103 8 0.3586705818046454 1103 9 -0.009126418884925158 1103 10-11 -0.1540841608623964 1103 12-13 0.11155238522349009 1103 14-15 0.1466153530652008 1103 16-17 0.43812316605964696 1103 18-19 0.32035644577783984 1103 20-21 0.42423216643197037 1103 22-23 0.17489873389342137 1103 24-25 0.016237575461786946 1103 26-27 -0.09122502344901307 1103 28-29 -0.0055825118750618685 1103 30-31 0.5503965291985864 1103 32-33 0.7678508454360795 1103 34-35 0.8405228170463062 1103 36-37 0.5957603784712333 1103 38-39 0.8422400704288648 1103 40-41 0.8450019381412375 1103 42-43 0.8013231430136472 1103 44-45 1.242598904748327 1103 46-47 1.1830387232392567 1103 48-49 1.564168009938527 1103 50-51 1.7616993992608059 1103 52-53 2.274825341929116 1103 54-55 1.8888329093450338 1103 56-57 1.868877733140522 1103 58-59 2.2071180382394857 1103 60-61 1.4813590980399916 1103 62-63 1.386992019485735 1103 64-65 1.7677977840077652 1103 66-67 2.1907672103530196 1103 68-69 1.4592779072853048 1103 70-71 1.0665522120847868 1103 72-73 1.053505545556778 1103 74-75 0.9247054876487582 1103 76-77 1.3486040365833212 1103 78-79 2.088402658496147 1103 80-81 1.7814639370911145 1103 82-83 1.6371544114263052 1103 84-85 1.5413755985378579 1103 86-87 1.6824426194419644 1103 88-89 1.575888397523869 1103 90-91 2.113580854604269 1103 92-93 2.283314272882837 1103 94-95 2.0176915381477336 1103 96-97 1.5622785801740804 1103 98-99 2.055830348450165 1103 100-101 2.330930976081774 1104 1 0.10798278013811924 1104 2 0.20802141253655648 1104 3 0.10085810649658455 1104 4 0.020066963568112328 1104 5 0.3448822317100877 1104 6 0.08354148284148977 1104 7 0.32118550581970595 1104 8 0.2679408376787009 1104 9 0.20662058736889577 1104 10-11 0.2483798845912517 1104 12-13 0.5236930863618028 1104 14-15 0.5174551802466425 1104 16-17 -0.08688225918054826 1104 18-19 0.014954879363038742 1104 20-21 -0.12923110962398 1104 22-23 0.1548352092628278 1104 24-25 0.005071444743094844 1104 26-27 0.2593145413480755 1104 28-29 0.5488079870497558 1104 30-31 0.36608632382857564 1104 32-33 0.5378107972994002 1104 34-35 0.47703172430428253 1104 36-37 0.6545107976943427 1104 38-39 0.8568586267231311 1104 40-41 1.380305455669692 1104 42-43 1.4268434728682493 1104 44-45 1.1711731516263484 1104 46-47 1.8286225777244525 1104 48-49 2.1664683117791625 1104 50-51 2.3408289934528383 1104 52-53 2.3918527048678158 1104 54-55 2.3746976926313437 1104 56-57 1.861864377185583 1104 58-59 2.0341712450496416 1104 60-61 1.7106198844039007 1104 62-63 1.9326725418870474 1104 64-65 2.6145692728909573 1104 66-67 1.9437806846768275 1104 68-69 2.0960926008085536 1104 70-71 1.4386151843272863 1104 72-73 1.1682345794694626 1104 74-75 1.4176921316938156 1104 76-77 1.1354315534015633 1104 78-79 1.4591931653122252 1104 80-81 1.4824444259545793 1104 82-83 1.5927167622322003 1104 84-85 1.3878906215211515 1104 86-87 1.6998724365620497 1104 88-89 1.409096785931606 1104 90-91 1.1302807303169509 1104 92-93 1.2582200709849936 1104 94-95 1.5020175260346527 1104 96-97 0.6649043964261274 1104 98-99 0.7022921054388611 1104 100-101 1.0930687179981646 1105 1 0.05090792707550662 1105 2 -0.10338870064166272 1105 3 0.03212541110628919 1105 4 -0.06105218929534573 1105 5 -0.10506190779542379 1105 6 0.12526684350599027 1105 7 0.13223131142518696 1105 8 0.21237178925008493 1105 9 0.022135232533322835 1105 10-11 0.5823284929363268 1105 12-13 0.374035535480175 1105 14-15 0.15628065263211965 1105 16-17 0.2415045868873591 1105 18-19 0.41880196733117714 1105 20-21 0.34934760509890594 1105 22-23 0.45655474100616544 1105 24-25 0.1851365343627549 1105 26-27 0.2819501852009978 1105 28-29 -0.20647447275497655 1105 30-31 -0.4001010759868393 1105 32-33 -0.6345066846066558 1105 34-35 -0.8899960116455858 1105 36-37 -0.4753527084017364 1105 38-39 -1.3971123258197409 1105 40-41 -1.7795284200771349 1105 42-43 -2.115804800313853 1105 44-45 -1.9010326092907377 1105 46-47 -0.6628040507304007 1105 48-49 -1.5779577823579203 1105 50-51 -2.2198160738226242 1105 52-53 -1.9852056329181629 1105 54-55 -2.029829436139238 1105 56-57 -1.846956723509031 1105 58-59 -2.1135433321107016 1105 60-61 -2.0072117569231516 1105 62-63 -1.458520929721253 1105 64-65 -1.7774742497203988 1105 66-67 -2.042691360788755 1105 68-69 -2.5569408669137985 1105 70-71 -2.4153217661754596 1105 72-73 -2.2556979993424093 1105 74-75 -2.6860869521940742 1105 76-77 -2.587524681115365 1105 78-79 -3.073254495833158 1105 80-81 -3.4324776122248544 1105 82-83 -3.7874017591647977 1105 84-85 -3.790571514778456 1105 86-87 -3.182286207242292 1105 88-89 -2.8813777853998133 1105 90-91 -2.6682328947862715 1105 92-93 -3.0233653955715774 1105 94-95 -3.7596767470503067 1105 96-97 -2.896177839936623 1105 98-99 -2.951397115914421 1105 100-101 -3.077621523416319 1106 1 -0.6331256863698727 1106 2 -0.4109517258517492 1106 3 -0.4250174460365699 1106 4 -0.32491773551382863 1106 5 -0.14875938678702028 1106 6 -0.08313651783854681 1106 7 -0.17701238605380354 1106 8 0.08800204135092571 1106 9 -0.31231854897928457 1106 10-11 -0.13111688521493647 1106 12-13 -0.27722496872151225 1106 14-15 -0.08825716249392457 1106 16-17 -0.6324449929445777 1106 18-19 0.18686919422193426 1106 20-21 -0.10947592431286068 1106 22-23 -0.40058811613669576 1106 24-25 -0.662762625301113 1106 26-27 -0.4071254450511077 1106 28-29 -0.1972307752759832 1106 30-31 -0.2076641011969187 1106 32-33 0.15120760110762888 1106 34-35 -0.2143657595447479 1106 36-37 -0.6644283386538419 1106 38-39 -0.5920703090130175 1106 40-41 -0.8963351427662118 1106 42-43 -0.6225274893894834 1106 44-45 -1.5438897521478765 1106 46-47 -2.3594427061925884 1106 48-49 -1.7384619840385866 1106 50-51 -2.3214967460915332 1106 52-53 -1.5801636161114416 1106 54-55 -1.9096613689123458 1106 56-57 -1.2805701688871807 1106 58-59 -1.096736609421626 1106 60-61 -1.7467075552424767 1106 62-63 -0.8644032826624297 1106 64-65 -0.4127683673674589 1106 66-67 -0.6872291759148048 1106 68-69 -0.791394648426401 1106 70-71 -1.50439739642756 1106 72-73 -1.434689595981066 1106 74-75 -0.8835659437907104 1106 76-77 -1.6497095550649412 1106 78-79 -1.5564477731440824 1106 80-81 -0.4173515618046899 1106 82-83 -1.5058891541227801 1106 84-85 -2.0040168929297195 1106 86-87 -1.34195007278851 1106 88-89 -1.589781146744354 1106 90-91 -2.616132054450137 1106 92-93 -1.7510964880085496 1106 94-95 -2.1756431336049253 1106 96-97 -1.5003795206088917 1106 98-99 -1.2043382923850068 1106 100-101 -0.9658568175339646 1107 1 -0.411022299560603 1107 2 -0.5350158969747412 1107 3 -0.5086181590490462 1107 4 -0.11261827027318816 1107 5 -0.8033048413324764 1107 6 0.058931218346835124 1107 7 -0.4172975910448926 1107 8 -0.005224340110750347 1107 9 -0.3868283529008565 1107 10-11 -0.25437891730049955 1107 12-13 0.0984434804763552 1107 14-15 0.4699424809998334 1107 16-17 0.017733260174857435 1107 18-19 -0.41625023536808925 1107 20-21 -0.6047522166479737 1107 22-23 0.15912667887221943 1107 24-25 0.9929414745206344 1107 26-27 -0.2106013808799858 1107 28-29 -0.8044500266128836 1107 30-31 -1.0153289853323955 1107 32-33 -0.7137656609244516 1107 34-35 -0.82105025152336 1107 36-37 -0.8707563243935894 1107 38-39 0.031013469953116157 1107 40-41 -0.27672729962895914 1107 42-43 -1.0936504840418948 1107 44-45 -2.4525350284402094 1107 46-47 -2.2567689094011385 1107 48-49 -2.7814209858211214 1107 50-51 -3.703136674790283 1107 52-53 -3.057525469943883 1107 54-55 -3.0949554865594067 1107 56-57 -2.733511345357769 1107 58-59 -2.8658988197603072 1107 60-61 -3.601431262907365 1107 62-63 -2.6421216427337306 1107 64-65 -2.305994748829132 1107 66-67 -3.276266609069882 1107 68-69 -3.438720851634958 1107 70-71 -3.7452173607769375 1107 72-73 -4.166062145000673 1107 74-75 -3.7534411666071144 1107 76-77 -3.1308147244054076 1107 78-79 -3.707517291860661 1107 80-81 -3.188207176777947 1107 82-83 -3.372644947349162 1107 84-85 -3.4267441656569915 1107 86-87 -3.665033851754643 1107 88-89 -3.6014567260669885 1107 90-91 -2.724509951063329 1107 92-93 -2.8047506769568535 1107 94-95 -3.5214541852983317 1107 96-97 -2.756885759468073 1107 98-99 -2.808348987572174 1107 100-101 -2.3556963897264787 1108 1 -0.8352647238030286 1108 2 0.1619537999949543 1108 3 -0.5995272499581361 1108 4 -0.264133421788344 1108 5 -0.13663817466581207 1108 6 -0.4865233271077116 1108 7 -0.5536612274085329 1108 8 -0.8991637340501484 1108 9 -0.4474344135069117 1108 10-11 -0.951348614270195 1108 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58-59 1.0105552070604702 2316 60-61 0.9848471693368346 2316 62-63 0.8851436754851285 2316 64-65 1.039007844339718 2316 66-67 1.5054070961979527 2316 68-69 1.7008101552554606 2316 70-71 1.6355709625541728 2316 72-73 1.366130194037634 2316 74-75 1.5900425705196568 2316 76-77 1.7759052874490813 2316 78-79 1.8047632149186263 2316 80-81 1.556458304440099 2316 82-83 1.6294481103673668 2316 84-85 0.9786236938673483 2316 86-87 0.8729931172014211 2316 88-89 1.0081046579442692 2316 90-91 1.4730524702154746 2316 92-93 1.3028658304151541 2316 94-95 1.084532330223638 2316 96-97 1.0232505671230214 2316 98-99 1.1489192942682358 2316 100-101 1.1465263264326673 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 1.0 5 8.0 6 10.0 7 44.0 8 103.0 9 191.0 10 315.0 11 564.0 12 854.0 13 1159.0 14 1579.0 15 2330.0 16 3022.0 17 4095.0 18 3711.0 19 2750.0 20 2663.0 21 2686.0 22 2624.0 23 2798.0 24 3031.0 25 3367.0 26 3855.0 27 4543.0 28 5666.0 29 6895.0 30 8600.0 31 10615.0 32 13166.0 33 16836.0 34 23105.0 35 31947.0 36 49444.0 37 57615.0 38 26538.0 39 3869.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 53.29804939751015 18.678849610545235 12.445909060648425 15.577191931296186 2 16.781718559228715 31.743676581644703 29.496754704929323 21.97785015419726 3 38.51507193993191 24.21561677300397 24.00560475805351 13.263706529010614 4 12.597471723220227 17.035262807717896 46.597471723220224 23.76979374584165 5 12.636726546906187 44.07950765136393 33.08183632734531 10.201929474384565 6 25.027611443779108 45.196274118429805 11.8020625415835 17.974051896207584 7 28.750166333998667 36.62375249500998 18.330671989354624 16.295409181636728 8 22.22222222222222 25.909181636726547 18.236526946107784 33.63206919494345 9 18.144045242847636 32.24451097804391 22.14337990685296 27.468063872255488 10-11 28.484863606121092 22.612774451097806 25.379740518962073 23.52262142381903 12-13 39.69444444444444 22.224550898203592 18.798902195608783 19.282102461743182 14-15 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32.296393927139654 17.181475213137563 40-41 18.168102731295118 32.77221464453242 26.969293722346055 22.090388901826408 42-43 18.585678447134292 34.991049861256215 19.589990484239106 26.83328120737039 44-45 25.711586000845088 24.302055156856678 21.970082612181887 28.01627623011635 46-47 28.487591634271343 31.699344774413767 21.977753269890062 17.83531032142483 48-49 29.284321813996193 29.66889113627593 22.82881342399766 18.217973625730217 50-51 18.602105823450156 30.196443719955425 35.00474059781433 16.196709858780086 52-53 24.41021631916433 36.51327772187061 19.927477773434575 19.149028185530483 54-55 17.971190472230077 30.184966483141768 23.805785192701144 28.03805785192701 56-57 27.885136866201517 23.476371573354097 26.208704736997962 22.429786823446427 58-59 34.06102204692186 23.924728052474766 21.70556305073637 20.308686849867 60-61 21.710758318750706 28.229524994510726 32.2198971342644 17.839819552474168 62-63 18.288384348338138 26.26658659964103 30.931045323814004 24.513983728206824 64-65 17.90277837906242 37.54641454894217 24.970777566495606 19.580029505499812 66-67 22.47572670112804 24.393458774502136 22.20961079508576 30.92120372928407 68-69 19.020060701259283 29.636291632278162 23.16831287771891 28.175334788743644 70-71 28.316188593798614 27.965640644262873 22.67913798811764 21.039032773820875 72-73 25.39408748184877 22.987563186232844 31.009199948769044 20.60914938314934 74-75 18.553756742775686 34.99157516895404 26.583543884803536 19.87112420346674 76-77 22.245490655293782 27.157366316475823 23.515625519797204 27.08151750843319 78-79 21.88936719472518 28.102316050006987 24.707749219888356 25.300567535379475 80-81 25.96012940892722 23.54976338791906 25.89010221309226 24.60000499006146 82-83 24.312743785294288 23.080531275210205 30.674406806443834 21.932318133051673 84-85 19.77957271838136 26.594987325264974 29.50518632858502 24.120253627768648 86-87 16.602793136256878 30.385004358037754 24.44410068065231 28.56810182505306 88-89 17.780136028170375 31.44117006182645 19.912208769475665 30.866485140527512 90-91 22.743649283423597 22.124046893504904 21.583786876738216 33.54851694633329 92-93 25.9444875989481 28.93101951267384 21.1672008769143 23.957292011463757 94-95 27.747088695605886 28.22098135201625 20.247309091453463 23.7846208609244 96-97 19.013242268135553 30.0235702725136 29.133821594494837 21.82936586485601 98-99 23.318490080193488 34.72938648071847 16.285715711503048 25.666407727584996 100-101 18.162431194720813 29.50713046429112 18.89119741563296 33.43924092535511 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10.0 1 5.0 2 4.0 3 9.0 4 7.5 5 8.0 6 10.0 7 13.0 8 17.0 9 25.0 10 27.0 11 32.5 12 44.5 13 49.0 14 52.5 15 66.0 16 94.0 17 121.5 18 152.0 19 189.5 20 240.5 21 272.5 22 319.5 23 411.5 24 479.0 25 545.5 26 612.0 27 711.5 28 856.5 29 1140.0 30 1500.0 31 1883.0 32 2391.0 33 3002.0 34 3756.0 35 4032.5 36 4124.5 37 4334.5 38 4632.0 39 4561.0 40 3745.0 41 3278.0 42 3307.5 43 3506.0 44 4189.0 45 5722.5 46 10842.5 47 30015.0 48 61411.0 49 56324.0 50 26349.0 51 15847.5 52 11869.0 53 7479.0 54 4640.5 55 3429.5 56 2509.0 57 1805.0 58 1103.0 59 865.0 60 604.5 61 370.0 62 277.0 63 179.0 64 102.5 65 56.0 66 18.0 67 6.5 68 4.5 69 2.0 70 2.0 71 2.0 72 1.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.059880239520958084 2 0.0036593479707252166 3 0.0469061876247505 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0036593479707252166 16-17 0.0 18-19 0.0 20-21 0.01413838988689288 22-23 0.0 24-25 0.0 26-27 0.006487025948103793 28-29 0.015635395874916833 30-31 0.010479041916167664 32-33 0.003992015968063873 34-35 0.005156353958749168 36-37 0.025948103792415172 38-39 0.04940119760479042 40-41 0.00332667997338656 42-43 0.015302727877578179 44-45 0.012974051896207586 46-47 0.0054890219560878245 48-49 0.0013306719893546241 50-51 0.00166333998669328 52-53 8.3166999334664E-4 54-55 0.00166333998669328 56-57 0.016966067864271454 58-59 0.012142381902860945 60-61 0.004657351962741184 62-63 0.006154357950765137 64-65 0.10545575515635397 66-67 0.05555555555555555 68-69 0.037258815701929474 70-71 0.02345309381237525 72-73 1.6633399866932801E-4 74-75 1.6633399866932801E-4 76-77 6.653359946773121E-4 78-79 6.653359946773121E-4 80-81 8.3166999334664E-4 82-83 8.3166999334664E-4 84-85 6.653359946773121E-4 86-87 0.0019960079840319364 88-89 1.6633399866932801E-4 90-91 0.0013306719893546241 92-93 1.6633399866932801E-4 94-95 0.0014970059880239522 96-97 0.003160345974717232 98-99 0.004823685961410512 100-101 0.007817697937458417 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 300600.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.873253493013973 #Duplication Level Percentage of deduplicated Percentage of total 1 75.6668835393624 12.767465069860279 2 8.773486327162319 2.9607451763140387 3 3.783442755466178 1.9151696606786426 4 2.2180162063050806 1.4970059880239521 5 1.484592180753534 1.2524950099800398 6 1.015358529997437 1.027944111776447 7 0.8182015338814299 0.9664005322687957 8 0.6861063464837049 0.9261477045908184 9 0.4534610910668165 0.688622754491018 >10 4.264505825989235 14.520625415834997 >50 0.4633189408726169 5.488689288090486 >100 0.2779913645235701 9.599467731204259 >500 0.04534610910668165 5.47538256819694 >1k 0.035488259300881286 15.363273453093813 >5k 0.009857849805800359 10.50964737192282 >10k+ 0.003943139922320144 15.040918163672654 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 35196 11.708582834331336 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 10017 3.3323353293413174 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 8088 2.69061876247505 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 6125 2.037591483699268 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 5879 1.9557551563539586 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 5875 1.9544244843646041 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 5625 1.87125748502994 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 4503 1.498003992015968 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3658 1.2168995342648037 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3623 1.2052561543579507 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3460 1.1510312707917498 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 3371 1.1214238190286094 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 3034 1.0093147039254824 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2948 0.9807052561543579 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2733 0.9091816367265468 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2621 0.8719228210246174 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2404 0.7997338656021291 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2281 0.7588157019294743 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 2112 0.7025948103792415 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 1918 0.6380572188955422 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1886 0.6274118429807052 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1761 0.5858283433133733 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 1672 0.5562208915502328 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 1181 0.39288090485695276 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1016 0.3379906852960745 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 990 0.32934131736526945 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 982 0.32667997338656024 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 931 0.3097139055222887 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 905 0.3010645375914837 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 894 0.29740518962075846 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 882 0.2934131736526946 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 869 0.28908848968729206 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 812 0.27012641383898867 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 770 0.25615435795076513 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 763 0.25382568196939453 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 741 0.24650698602794413 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 688 0.22887558216899534 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 649 0.21590153027278777 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 632 0.21024617431803064 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 583 0.19394544244843645 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 582 0.19361277445109779 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 569 0.18928809048569528 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 565 0.18795741849634065 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 549 0.18263473053892215 No Hit GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 543 0.18063872255489022 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 529 0.17598137059214902 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 522 0.17365269461077845 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 509 0.16932801064537592 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 491 0.16333998669328012 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 487 0.1620093147039255 No Hit GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 475 0.1580172987358616 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 462 0.1536926147704591 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 455 0.1513639387890885 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 444 0.14770459081836326 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 443 0.14737192282102463 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 439 0.14604125083167 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 438 0.14570858283433133 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 433 0.14404524284763806 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 432 0.1437125748502994 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 428 0.14238190286094476 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 384 0.12774451097804393 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 382 0.1270791749833666 No Hit GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 375 0.124750499001996 No Hit ATTTAAAATTGCCTCTGGTGATTTAACCTTTTATCCCTTATTAGAAAAAG 374 0.12441783100465735 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 362 0.12042581503659347 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 360 0.11976047904191617 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 357 0.11876247504990019 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 346 0.11510312707917497 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 335 0.11144377910844977 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 332 0.1104457751164338 No Hit ATATATAGGAAAAGGGAAGGTAGAAGAGCTGAAGGCACTCGTGGAAGAGC 326 0.10844976713240186 No Hit TGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT 307 0.1021290751829674 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 9.98003992015968E-4 0.0 10-11 0.0 0.0 0.0 0.002162341982701264 0.0 12-13 0.0 0.0 0.0 0.003825681969394544 0.0 14-15 0.0 0.0 0.0 0.00582168995342648 0.0 16-17 0.0 0.0 0.0 0.01014637391882901 0.0 18-19 0.0 0.0 0.0 0.01413838988689288 0.0 20-21 0.0 0.0 0.0 0.01912840984697272 0.0 22-23 0.0 0.0 0.0 0.02827677977378576 0.0 24-25 0.0 0.0 0.0 0.03825681969394544 0.0 26-27 0.0 0.0 0.0 0.04923486360612109 0.0 28-29 0.0 0.0 0.0 0.08150365934797073 0.0 30-31 0.0 0.0 0.0 0.12857618097139056 0.0 32-33 0.0 0.0 0.0 0.21773120425815035 0.0 34-35 0.0 0.0 0.0 0.29491017964071853 0.0 36-37 0.0 0.0 0.0 0.35212907518296743 0.0 38-39 0.0 0.0 0.0 0.40818363273453095 0.0 40-41 0.0 0.0 0.0 0.48353293413173654 0.0 42-43 0.0 0.0 0.0 0.6570192947438457 0.0 44-45 0.0 0.0 0.0 0.9013639387890885 0.0 46-47 0.0 0.0 0.0 1.1636726546906186 0.0 48-49 0.0 0.0 0.0 1.6320691949434463 0.0 50-51 0.0 0.0 0.0 2.022787757817698 0.0 52-53 0.0 0.0 0.0 2.4135063206919494 0.0 54-55 0.0 0.0 0.0 3.1345642049234863 0.0 56-57 0.0 0.0 0.0 4.1437125748503 0.0 58-59 0.0 0.0 0.0 5.075016633399867 0.0 60-61 0.0 0.0 0.0 5.959747172322023 0.0 62-63 0.0 0.0 0.0 7.7822687957418495 0.0 64-65 0.0 0.0 0.0 8.556054557551564 0.0 66-67 0.0 0.0 0.0 10.195941450432468 0.0 68-69 0.0 0.0 0.0 12.040252827677978 0.0 70-71 0.0 0.0 0.0 12.891550232867598 0.0 72-73 0.0 0.0 0.0 14.435129740518963 0.0 74-75 0.0 0.0 0.0 16.922155688622755 0.0 76-77 0.0 0.0 0.0 17.796407185628745 0.0 78-79 0.0 0.0 0.0 18.85911510312708 0.0 80-81 0.0 0.0 0.0 20.798735861610112 0.0 82-83 0.0 0.0 0.0 22.916666666666664 0.0 84-85 0.0 0.0 0.0 24.862109115103127 0.0 86-87 0.0 0.0 0.0 27.120924817032602 0.0 88-89 0.0 0.0 0.0 28.498170326014638 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAAGA 20 0.0020713285 71.26086 2 CTGTCGA 20 0.002076805 71.21345 9 GCTGTCG 20 0.002076805 71.21345 8 CCCGGCG 30 1.3029733E-4 63.300842 8 CCCCTAT 125 0.0 60.870056 1 TCTTGCT 40 7.525163E-6 59.38405 2 CCTCAGG 50 4.226913E-7 57.00869 2 TACTGTG 75 3.8198777E-11 56.97076 7 CTGTGCG 60 2.3530447E-8 55.388237 9 TAGTACT 60 2.3530447E-8 55.388237 4 CCCTACG 130 0.0 54.779575 9 TACCCCG 35 2.7916895E-4 54.257866 5 CTACCCC 35 2.7916895E-4 54.257866 4 CTTGCTT 45 1.5148831E-5 52.76826 3 CTCAGGA 55 8.180523E-7 51.808834 3 TATTCTC 65 4.422509E-8 51.1276 5 ACTGTGC 65 4.422509E-8 51.1276 8 TCCTTGT 65 4.422509E-8 51.1276 8 TTTAAGC 40 5.377745E-4 47.50724 2 AGATCCA 20 5.386487E-4 47.499332 28-29 >>END_MODULE