FastQCFastQC Report
Wed 25 May 2016
SRR1003839_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1003839_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences485584
Sequences flagged as poor quality0
Sequence length121
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT191253.9385564598504073No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC117942.4288279679725857No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT103412.1296006458202905No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT99762.0544334244950413No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT66981.379369995716498No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC45420.9353685459158456No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30030.6184305907937658No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA27420.5646808791063956No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC26420.544087119839204No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT26410.5438811822465319No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22670.46686052258723515No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC21990.45285676628554483No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG21190.4363817588717915No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG20030.41249299812184914No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC19920.41022768460245806No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC19460.4007545553395499No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT19230.39601799070809585No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA16050.3305298362384263No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA15230.31364295363932915No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG14600.3006688853009984No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12520.25783386602523967No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA11450.23579854360934466No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT10500.21623447230551254No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA10030.20655540544993245No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC9950.20490790470855713No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT9670.19914165211374346No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT9590.19749415137236812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8890.18307851988533394No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA8840.18204883192197435No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG7910.1628966358034861No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7880.16227882302547036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7510.15465913209660945No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA7210.14848100431645195No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG6890.1418910013509506No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG6380.13138818412468284No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6270.12912287060529176No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT6040.12438630597383768No Hit
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC5770.11882599097169594No Hit
CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5660.11656067745230486No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC5600.11532505189627335No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT5170.10646973541138094No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCG209.749335E-486.1669549
GTACCCG603.9407496E-757.6006361
TACCTGG3250.054.8676452
TACCCGG701.1526226E-649.3050272
ACCTGGG3900.044.1973883
TACACGG600.001544075938.348352
ACACGGG600.001552801938.30443
TCGCAAC452.943628E-431.908699102-103
TAGACAG1803.0737283E-828.734295
TATGTAC2253.074092E-1028.0898954
ACCCGGG1256.187883E-527.579173
GTACCTG6750.027.3069691
CCTGGGG3500.026.2658774
TCTCGGG1900.025.714989114-115
CATGGGG4000.024.4190584
GTATTGG1451.6680114E-423.8347451
GCTTATG2202.0996595E-723.5638981
GAGTACT12450.023.54625512-13
TCGCCGT2850.023.172173104-105
ATACTGC1250.00199767922.9874326
Produced by FastQC (version 0.11.5)