##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1003839_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 485584 Sequences flagged as poor quality 0 Sequence length 121 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.058218557448352 31.0 31.0 34.0 28.0 34.0 2 30.848794441332497 33.0 31.0 34.0 27.0 34.0 3 31.035651916043363 33.0 31.0 34.0 27.0 34.0 4 34.42149041154568 37.0 35.0 37.0 32.0 37.0 5 34.32628340307753 37.0 35.0 37.0 30.0 37.0 6 34.41172896965303 37.0 35.0 37.0 31.0 37.0 7 34.367897212428744 37.0 35.0 37.0 30.0 37.0 8 34.46059796039408 37.0 35.0 37.0 31.0 37.0 9 35.96513270618472 39.0 37.0 39.0 31.0 39.0 10-11 35.880698293189226 39.0 37.0 39.0 30.5 39.0 12-13 35.86468046723121 39.0 37.0 39.0 30.5 39.0 14-15 36.887310949289926 40.0 37.0 41.0 31.0 41.0 16-17 36.85569129131108 40.0 37.0 41.0 31.0 41.0 18-19 36.79470287324129 40.0 37.0 41.0 30.5 41.0 20-21 36.757870934791924 40.0 37.0 41.0 30.5 41.0 22-23 36.56226626083232 40.0 36.5 41.0 30.0 41.0 24-25 36.45335101650795 39.5 36.0 41.0 29.5 41.0 26-27 36.30971984249893 39.0 36.0 41.0 29.5 41.0 28-29 36.17745436422946 39.0 36.0 41.0 29.0 41.0 30-31 35.85340641372039 39.0 36.0 40.5 27.0 41.0 32-33 35.62302505848628 39.0 35.0 40.0 27.0 41.0 34-35 35.43378282645227 39.0 35.0 40.0 25.5 41.0 36-37 35.38685479752216 38.5 35.0 40.0 26.0 41.0 38-39 35.30505123727306 38.5 35.0 40.0 25.5 41.0 40-41 35.500937016046656 39.0 35.0 41.0 26.0 41.0 42-43 35.368437798609506 39.0 35.0 41.0 25.0 41.0 44-45 35.135168168638174 39.0 35.0 41.0 24.5 41.0 46-47 34.800944841675175 39.0 35.0 40.0 23.5 41.0 48-49 34.504893077201885 38.5 34.0 40.0 22.0 41.0 50-51 33.700346387030876 37.5 33.5 39.5 19.5 40.5 52-53 33.80900112030051 38.0 33.5 40.0 19.5 40.5 54-55 34.14719801311411 38.0 34.0 40.0 19.5 41.0 56-57 33.72811192296287 38.0 33.5 40.0 14.5 41.0 58-59 33.41904902138456 38.0 33.0 40.0 12.5 41.0 60-61 32.86277142574714 38.0 32.0 40.0 8.0 41.0 62-63 32.779391413226136 37.0 32.0 40.0 8.0 41.0 64-65 32.46666076312235 37.0 31.5 39.5 7.5 41.0 66-67 31.98570175294079 36.0 31.0 39.0 6.5 41.0 68-69 31.602361280437577 36.0 31.0 39.0 2.0 40.5 70-71 30.976941167748528 35.0 30.0 38.5 2.0 40.0 72-73 30.552505848627632 35.0 29.5 37.0 2.0 40.0 74-75 30.14293304556987 35.0 29.0 37.0 2.0 39.0 76-77 29.70607968961086 34.5 29.0 36.5 2.0 39.0 78-79 28.993540767405847 34.0 28.5 36.0 2.0 39.0 80-81 28.46258010972355 34.0 26.5 35.5 2.0 37.5 82-83 27.972217989060596 34.0 26.0 35.0 2.0 37.0 84-85 27.682370094566544 34.0 26.0 35.0 2.0 37.0 86-87 27.63490971695937 34.0 26.0 35.0 2.0 36.0 88-89 27.248850868232893 34.0 25.5 35.0 2.0 36.0 90-91 26.897574878908696 33.0 25.0 35.0 2.0 35.5 92-93 26.56311987215394 33.0 25.0 35.0 2.0 35.0 94-95 26.303293559919602 33.0 24.0 35.0 2.0 35.0 96-97 26.04408917921513 33.0 23.0 35.0 2.0 35.0 98-99 25.700379337045703 32.5 20.0 35.0 2.0 35.0 100-101 25.354428893868 32.0 19.0 35.0 2.0 35.0 102-103 24.895212774720747 32.0 18.0 35.0 2.0 35.0 104-105 24.377131454084157 32.0 12.5 34.5 2.0 35.0 106-107 23.833744522060037 31.0 7.0 34.0 2.0 35.0 108-109 23.615647344228805 31.0 4.5 34.0 2.0 35.0 110-111 23.276935607433522 31.0 2.0 34.0 2.0 35.0 112-113 22.610175582391513 31.0 2.0 34.0 2.0 35.0 114-115 21.98589224521401 30.0 2.0 34.0 2.0 35.0 116-117 21.20804742330884 29.0 2.0 34.0 2.0 35.0 118-119 20.381385095060793 29.0 2.0 34.0 2.0 35.0 120-121 18.77793852350984 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9260315015049692 1101 2 1.0099535768896004 1101 3 0.969136116375612 1101 4 1.2643422009883736 1101 5 1.367585028547964 1101 6 1.4072977775249669 1101 7 1.3506753983543476 1101 8 1.3376177450393385 1101 9 1.4133626189924016 1101 10-11 1.432798118801763 1101 12-13 1.1944371787055985 1101 14-15 1.5806577781156506 1101 16-17 1.7084745124801657 1101 18-19 1.6758546476540417 1101 20-21 1.5195577917838747 1101 22-23 1.5040390838744244 1101 24-25 1.6702189678332076 1101 26-27 1.6923720916575533 1101 28-29 1.6957750512979501 1101 30-31 1.7348015729938169 1101 32-33 1.4593292768780586 1101 34-35 1.7554537725706538 1101 36-37 1.223790429876182 1101 38-39 1.3714035156509397 1101 40-41 1.494009686750239 1101 42-43 1.6655710092800504 1101 44-45 1.4232546825718657 1101 46-47 1.6001104412713403 1101 48-49 1.5830878180291137 1101 50-51 1.8047769445889514 1101 52-53 1.717840763380316 1101 54-55 1.7577728191838204 1101 56-57 1.7066848761654256 1101 58-59 1.9010186365513349 1101 60-61 1.6297241089944663 1101 62-63 1.6866237215391848 1101 64-65 1.9865739876549924 1101 66-67 1.472808512620233 1101 68-69 1.3860036345140756 1101 70-71 0.9568863593271502 1101 72-73 1.071971055346662 1101 74-75 1.1360004859985722 1101 76-77 1.219167796555542 1101 78-79 1.3554491268494466 1101 80-81 1.356734531796974 1101 82-83 1.4382037441808428 1101 84-85 0.957760629741994 1101 86-87 1.1154992640354031 1101 88-89 1.2063821772347474 1101 90-91 1.161584596693789 1101 92-93 1.0991493058581838 1101 94-95 0.892337228341006 1101 96-97 0.9651128300952045 1101 98-99 0.9727779629909818 1101 100-101 0.9777426513980032 1101 102-103 1.1475637099655671 1101 104-105 0.880771951298776 1101 106-107 0.8232151566972057 1101 108-109 0.3805014225680132 1101 110-111 -0.1581667523967667 1101 112-113 0.07176210933218741 1101 114-115 0.16753818302352386 1101 116-117 -0.32008942263098916 1101 118-119 -0.9349538744846733 1101 120-121 -0.7911949403825531 1102 1 0.7607770956490612 1102 2 1.0825774164307305 1102 3 0.9232349276172798 1102 4 1.1816462837059944 1102 5 1.3433686434293222 1102 6 1.2785777089552752 1102 7 1.4226591094358056 1102 8 1.2159173902562728 1102 9 1.4692165395079755 1102 10-11 1.3891518458321812 1102 12-13 1.3384913979477915 1102 14-15 1.2003165932631816 1102 16-17 1.4905758697695006 1102 18-19 1.490902729619414 1102 20-21 1.4098788324808496 1102 22-23 1.7134858609903674 1102 24-25 1.3131918401293845 1102 26-27 1.5510700631374803 1102 28-29 1.6169379874984813 1102 30-31 1.4654250291811834 1102 32-33 1.4477888818790134 1102 34-35 1.6294063307337368 1102 36-37 1.4009523100455894 1102 38-39 1.455057817350351 1102 40-41 1.4250891892583226 1102 42-43 1.4024239641781193 1102 44-45 1.5756540793321605 1102 46-47 1.783469271246716 1102 48-49 1.5508915264343202 1102 50-51 1.3416241130150652 1102 52-53 1.475255584704918 1102 54-55 1.4992477185534554 1102 56-57 1.7482950343169392 1102 58-59 1.6368643836752739 1102 60-61 1.8911189380584688 1102 62-63 1.7064785534240912 1102 64-65 1.7275913940859269 1102 66-67 1.7887082909260243 1102 68-69 1.6780794228304643 1102 70-71 1.4033687268621513 1102 72-73 1.5989848379995415 1102 74-75 1.9569543858998415 1102 76-77 1.661738870705566 1102 78-79 1.6841478578038114 1102 80-81 1.944757619820063 1102 82-83 1.7367133659987353 1102 84-85 1.3042147882262256 1102 86-87 1.199222280089245 1102 88-89 1.259086087081517 1102 90-91 1.202446130036524 1102 92-93 1.4073530764679703 1102 94-95 1.527440498782621 1102 96-97 1.442113412358946 1102 98-99 1.7725135935536827 1102 100-101 1.4609944316422663 1102 102-103 1.7129201023896279 1102 104-105 1.7478820063787524 1102 106-107 1.6110288467665335 1102 108-109 1.3403053334997423 1102 110-111 1.1852368504053317 1102 112-113 1.383428721750679 1102 114-115 0.9248933313561167 1102 116-117 0.870482606286906 1102 118-119 0.7240565664806518 1102 120-121 0.60389632312976 1103 1 0.8097049708927244 1103 2 1.0655727297731907 1103 3 0.8144090804935615 1103 4 1.1616263757801448 1103 5 1.0457202671695285 1103 6 1.26341035795366 1103 7 1.268351946729986 1103 8 1.08370387775161 1103 9 1.2645132920275728 1103 10-11 1.1316158664037061 1103 12-13 1.128732366800186 1103 14-15 1.3532488648404808 1103 16-17 1.0999263736888736 1103 18-19 0.9395008798412974 1103 20-21 1.1435916606505643 1103 22-23 1.0921968227920402 1103 24-25 1.144998899148085 1103 26-27 1.2472274187013284 1103 28-29 1.029359703727586 1103 30-31 1.291624689087449 1103 32-33 0.9762281834426219 1103 34-35 1.3648071851155592 1103 36-37 1.184878733669656 1103 38-39 0.6939985497961203 1103 40-41 0.9758772113229313 1103 42-43 1.1665026004851953 1103 44-45 1.038637779717881 1103 46-47 0.9993209571991812 1103 48-49 0.811144108243461 1103 50-51 0.8565592048818473 1103 52-53 1.0733000457393018 1103 54-55 0.7864133276115624 1103 56-57 0.5940314201989025 1103 58-59 0.8661767307367754 1103 60-61 0.6985533041154568 1103 62-63 0.971222861001543 1103 64-65 0.7284146697692293 1103 66-67 0.7383080586668882 1103 68-69 0.7831891237968307 1103 70-71 0.48754399955926075 1103 72-73 0.5748112242962513 1103 74-75 0.6156081124147086 1103 76-77 0.5405825520128538 1103 78-79 0.5748803035754513 1103 80-81 0.5594148302219502 1103 82-83 0.6388866479032629 1103 84-85 0.418762090287526 1103 86-87 0.8166894112741829 1103 88-89 0.6813871115101975 1103 90-91 0.6346513475720919 1103 92-93 0.44497490882664437 1103 94-95 0.23679307644426828 1103 96-97 0.35483374747569485 1103 98-99 0.7766030133205923 1103 100-101 0.35705224757690246 1103 102-103 0.27668975109768823 1103 104-105 0.19870113457750094 1103 106-107 0.03706468103305127 1103 108-109 -0.11911567765698905 1103 110-111 -0.44278562896093376 1103 112-113 -0.5184127613897971 1103 114-115 -0.8172326692245306 1103 116-117 -0.7777671552327341 1103 118-119 -0.9409282952007381 1103 120-121 -0.9675005496668874 1104 1 0.47731422539143864 1104 2 0.7549016145295191 1104 3 0.6657846753084158 1104 4 0.8113611020285774 1104 5 0.786069225217986 1104 6 0.813763144695443 1104 7 0.881735964670149 1104 8 0.5228221843752507 1104 9 0.9051521368561026 1104 10-11 0.9351136512311413 1104 12-13 0.6631906066951672 1104 14-15 0.8157522833166553 1104 16-17 1.1584151337391901 1104 18-19 1.0406905095519576 1104 20-21 1.0863171433394072 1104 22-23 0.968240141722184 1104 24-25 1.0317734985026803 1104 26-27 0.8750702234926635 1104 28-29 1.0331774579254756 1104 30-31 1.100381457670288 1104 32-33 1.0058950871236334 1104 34-35 0.8748575050849254 1104 36-37 0.5571507896779693 1104 38-39 0.671502241750396 1104 40-41 1.1162726606474465 1104 42-43 1.176232951084117 1104 44-45 1.2781225469880226 1104 46-47 1.3409115057962424 1104 48-49 1.1385055915886468 1104 50-51 1.1162649424522684 1104 52-53 1.265207828925874 1104 54-55 1.1420415067944134 1104 56-57 1.209048102362658 1104 58-59 1.3655559354624813 1104 60-61 1.5906032959111087 1104 62-63 1.7596738810748391 1104 64-65 1.5148802388771472 1104 66-67 1.229234055494544 1104 68-69 1.6151149565376244 1104 70-71 1.1817052422437193 1104 72-73 1.2300401254612687 1104 74-75 1.1609562500288888 1104 76-77 1.4261921346852802 1104 78-79 1.5026082475671387 1104 80-81 1.182261190228516 1104 82-83 1.2276357590994529 1104 84-85 1.2312080783638812 1104 86-87 1.686636903480057 1104 88-89 1.68705904718815 1104 90-91 1.4694049438090317 1104 92-93 0.8951034435310135 1104 94-95 1.3927521641213474 1104 96-97 1.1979776516489729 1104 98-99 1.136675211537515 1104 100-101 0.9734144419651294 1104 102-103 0.7702821593457863 1104 104-105 0.9551360741388422 1104 106-107 0.8268995002640303 1104 108-109 0.9339828291526118 1104 110-111 0.4733249655804457 1104 112-113 0.5459311646617273 1104 114-115 0.0900691958960742 1104 116-117 -0.04507284904665809 1104 118-119 -0.09969053334546274 1104 120-121 -0.16569832902476023 1105 1 0.474410853245665 1105 2 0.9494275482963452 1105 3 0.7089647751118342 1105 4 1.0161320078201967 1105 5 1.3723160118503799 1105 6 1.0637858272903316 1105 7 1.0446348002969401 1105 8 0.9243797225576174 1105 9 1.214262979136464 1105 10-11 1.0254206223486406 1105 12-13 0.9286072103546275 1105 14-15 0.8896068122747636 1105 16-17 1.020452030760211 1105 18-19 1.0744195281516866 1105 20-21 1.3444677557694646 1105 22-23 1.0412894385617477 1105 24-25 0.7727836300617312 1105 26-27 1.0036729756475111 1105 28-29 1.3430330454046882 1105 30-31 1.2796722485367624 1105 32-33 1.4309215501510053 1105 34-35 1.379231465465999 1105 36-37 0.7741778575745357 1105 38-39 1.1219710153781008 1105 40-41 0.734293982286637 1105 42-43 0.9551759421622918 1105 44-45 1.0192385306565512 1105 46-47 1.2528879158975528 1105 48-49 0.820155372323562 1105 50-51 0.874925913267333 1105 52-53 1.2805904086929942 1105 54-55 1.105843865784287 1105 56-57 1.2369287919135417 1105 58-59 1.0012205354927275 1105 60-61 1.2806872215122027 1105 62-63 0.9489450136924091 1105 64-65 1.0273821290933824 1105 66-67 0.9730076831975509 1105 68-69 0.17674356685064652 1105 70-71 0.45581684061057004 1105 72-73 0.7061629139082655 1105 74-75 0.7570661850054492 1105 76-77 1.175814091437129 1105 78-79 0.592934120847044 1105 80-81 0.38920206426450576 1105 82-83 0.18378652274681784 1105 84-85 0.4631300502374671 1105 86-87 0.25464310338932705 1105 88-89 0.5576699650771566 1105 90-91 0.31938226121414104 1105 92-93 0.019974908826640103 1105 94-95 0.05243763213888997 1105 96-97 -0.08584209733031045 1105 98-99 -0.0739289015730229 1105 100-101 0.2713200823703943 1105 102-103 0.011764844965021837 1105 104-105 -0.04403979158019666 1105 106-107 -0.774574868403743 1105 108-109 -0.9089780055668761 1105 110-111 -1.206521548860806 1105 112-113 -0.8589947388616359 1105 114-115 -1.3007745966337936 1105 116-117 -2.1238873054204674 1105 118-119 -1.7097956293684469 1105 120-121 -1.8572377211312165 1106 1 0.4239906851784383 1106 2 0.6418645230862587 1106 3 0.7031291075114616 1106 4 0.8889144541320562 1106 5 1.2746969642313317 1106 6 1.1043087031073213 1106 7 1.1550456312960122 1106 8 1.1519240736313776 1106 9 1.0764011677452388 1106 10-11 0.8869519015269773 1106 12-13 0.8568985268812384 1106 14-15 0.9114555517705654 1106 16-17 0.8401999299198764 1106 18-19 0.9628882489733499 1106 20-21 0.7996031432577979 1106 22-23 1.1384649754431635 1106 24-25 1.0905240595668673 1106 26-27 0.7657177935746802 1106 28-29 1.0879210005867535 1106 30-31 0.7354612307963251 1106 32-33 0.5549178153237406 1106 34-35 0.9472286643455519 1106 36-37 0.5478473253616514 1106 38-39 0.07346494628379219 1106 40-41 0.8499569141260466 1106 42-43 0.49331786559833546 1106 44-45 0.38375767164628627 1106 46-47 0.572775871079628 1106 48-49 1.152228201455209 1106 50-51 1.4096831494951516 1106 52-53 1.4151609035576058 1106 54-55 1.712029673440675 1106 56-57 1.179762592100282 1106 58-59 1.196318574708414 1106 60-61 1.4367329731461922 1106 62-63 1.2189263395560843 1106 64-65 1.3242775539299814 1106 66-67 1.5808274777820586 1106 68-69 1.0480320822568103 1106 70-71 0.9332211356619275 1106 72-73 0.8390293938335702 1106 74-75 0.9196945696926591 1106 76-77 0.686411663799408 1106 78-79 0.5484196483913948 1106 80-81 0.9098369848994246 1106 82-83 0.4922132267617556 1106 84-85 0.16481072250637396 1106 86-87 0.13244422383750276 1106 88-89 0.21728714528257242 1106 90-91 0.17251017904794352 1106 92-93 0.12275268660442151 1106 94-95 0.5366579869474783 1106 96-97 0.6739151388975095 1106 98-99 0.48122982858570396 1106 100-101 0.36912587135265085 1106 102-103 -0.22481311955411698 1106 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2205 30-31 1.3640305373068102 2205 32-33 1.6124723522900481 2205 34-35 1.5994186312413987 2205 36-37 1.6194987131895147 2205 38-39 1.5769175394422703 2205 40-41 1.9046148379016117 2205 42-43 1.7574486694350284 2205 44-45 1.811350830121789 2205 46-47 1.7543585041328527 2205 48-49 1.9291125915748992 2205 50-51 1.9051398170106495 2205 52-53 1.7463658097625157 2205 54-55 1.6800417267468433 2205 56-57 1.64936194699375 2205 58-59 1.7052312306798925 2205 60-61 1.7761417669667452 2205 62-63 1.4410573131576498 2205 64-65 1.4173553911254686 2205 66-67 1.6980076831975524 2205 68-69 1.8160483796848723 2205 70-71 1.7670467871346354 2205 72-73 1.3410559620366094 2205 74-75 1.4125474684279098 2205 76-77 1.4184611502606614 2205 78-79 2.043869949724048 2205 80-81 1.8190148984891046 2205 82-83 1.6892678061692763 2205 84-85 1.709520424569014 2205 86-87 1.8634666328010887 2205 88-89 2.0648892164140555 2205 90-91 1.7506656836740362 2205 92-93 0.9906968339603317 2205 94-95 1.55243763213889 2205 96-97 1.6693718064130039 2205 98-99 1.7704561251649444 2205 100-101 1.822389601086968 2205 102-103 1.75200548667625 2205 104-105 1.8079655560133858 2205 106-107 1.6222165754465259 2205 108-109 1.9038562190320505 2205 110-111 1.4080506436525617 2205 112-113 1.3922084696945127 2205 114-115 1.5941451894624663 2205 116-117 1.348305207948517 2205 118-119 1.5000974187598963 2205 120-121 1.363216824323331 2206 1 0.610828566398343 2206 2 1.0060699805501443 2206 3 0.995977332943955 2206 4 1.0595226224930343 2206 5 1.3077092702773427 2206 6 1.085332423456883 2206 7 1.3669414757761231 2206 8 1.1188939327360714 2206 9 0.9416588813175579 2206 10-11 1.1502388642521382 2206 12-13 1.1105635884114662 2206 14-15 1.2099306721557994 2206 16-17 1.2576959170787845 2206 18-19 1.4192642075965054 2206 20-21 1.3820090644277627 2206 22-23 1.5481136289120414 2206 24-25 1.4054339935779225 2206 26-27 1.409885797855047 2206 28-29 1.170478517975738 2206 30-31 1.4351580383583027 2206 32-33 1.421040519086894 2206 34-35 1.5025791191342037 2206 36-37 1.5443695297490265 2206 38-39 1.4625856882135224 2206 40-41 1.568514074818033 2206 42-43 1.5542506282164865 2206 44-45 1.9129265676691105 2206 46-47 1.8575805794798441 2206 48-49 1.8479478376242824 2206 50-51 1.882939792976515 2206 52-53 1.9479235217134843 2206 54-55 1.8026217904372857 2206 56-57 2.0230656061898173 2206 58-59 1.9410751290155233 2206 60-61 1.8263085037329603 2206 62-63 1.6332477235404 2206 64-65 1.7824548323319789 2206 66-67 2.040208608511506 2206 68-69 1.793002654755469 2206 70-71 1.6210845207163196 2206 72-73 1.3086744803325914 2206 74-75 1.5941283132275217 2206 76-77 1.694981308885911 2206 78-79 1.8586479344623754 2206 80-81 1.9212245361746163 2206 82-83 1.9030033105855502 2206 84-85 1.7618412200986668 2206 86-87 1.6898546037197946 2206 88-89 1.7237968652754354 2206 90-91 1.4304199347105104 2206 92-93 1.507896257141251 2206 94-95 1.544407228966385 2206 96-97 1.4982953778844923 2206 98-99 1.5887115478651808 2206 100-101 1.6063828715310002 2206 102-103 1.4493887709399083 2206 104-105 1.5305801687634926 2206 106-107 1.537255931331881 2206 108-109 1.4649149223908253 2206 110-111 1.7103819541134158 2206 112-113 1.4611473629228975 2206 114-115 1.120838093386034 2206 116-117 0.4051278961657836 2206 118-119 0.5875440930373692 2206 120-121 0.8296426489943585 2207 1 0.6784072609690597 2207 2 1.2396834980044709 2207 3 1.2321125074872228 2207 4 1.1740350163518585 2207 5 1.3497969278809094 2207 6 1.0832739278740746 2207 7 1.1664354289453485 2207 8 1.234063901572732 2207 9 1.2349859248032757 2207 10-11 1.3894903723860565 2207 12-13 1.3985742811524062 2207 14-15 1.6074933562053175 2207 16-17 1.6889821909158726 2207 18-19 1.7192556305315705 2207 20-21 1.8045710337218637 2207 22-23 1.8736461216930138 2207 24-25 1.5617224116812523 2207 26-27 1.6212975827422582 2207 28-29 1.544645827922274 2207 30-31 1.6260949397506508 2207 32-33 1.5219782781857347 2207 34-35 1.5242733753597264 2207 36-37 1.4475201715993862 2207 38-39 1.5963535928365644 2207 40-41 1.6115466390745468 2207 42-43 1.4625775287511331 2207 44-45 1.853320254949324 2207 46-47 1.8550732174991182 2207 48-49 1.6608977761553163 2207 50-51 1.6167048385779168 2207 52-53 1.6453075169103286 2207 54-55 1.585281824173741 2207 56-57 1.6881898306801872 2207 58-59 1.4094229004081527 2207 60-61 1.3979713107204077 2207 62-63 1.5501574070466972 2207 64-65 1.7459908702997922 2207 66-67 1.873842139117329 2207 68-69 1.5839281200612447 2207 70-71 1.4929145804698756 2207 72-73 1.864717022574638 2207 74-75 2.001561769498789 2207 76-77 2.0017757738200075 2207 78-79 2.125597644271682 2207 80-81 1.9286422678268487 2207 82-83 2.1515795178074377 2207 84-85 1.821062994407498 2207 86-87 2.097656873782981 2207 88-89 1.7784527820207288 2207 90-91 1.612107901088411 2207 92-93 1.8545168935594667 2207 94-95 1.7289456399221557 2207 96-97 1.73208186614821 2207 98-99 1.7720634648391922 2207 100-101 2.0510536551888308 2207 102-103 2.115420734801873 2207 104-105 1.9229337153798411 2207 106-107 1.6598436318207739 2207 108-109 2.2127477839855842 2207 110-111 2.0685158706871647 2207 112-113 1.7035595194829227 2207 114-115 1.9519270947297755 2207 116-117 1.8058612352171615 2207 118-119 1.3587008531940477 2207 120-121 1.3077525497863114 2208 1 0.46990648222773856 2208 2 0.9804395500743865 2208 3 0.979723406020117 2208 4 1.1389724286232834 2208 5 1.2434747020267025 2208 6 1.2705275668072957 2208 7 1.2111409493560643 2208 8 1.0921823599853866 2208 9 1.2571656313812412 2208 10-11 1.3019652956335719 2208 12-13 1.2085597959228593 2208 14-15 1.625093551050881 2208 16-17 1.5841725819529842 2208 18-19 1.4446002956728066 2208 20-21 1.65759562656514 2208 22-23 1.4912857343092654 2208 24-25 1.490312152805842 2208 26-27 1.358784844072467 2208 28-29 1.3361097975161087 2208 30-31 1.5127512231996718 2208 32-33 1.7934048810148369 2208 34-35 1.4968748200370428 2208 36-37 1.5013078027516045 2208 38-39 1.8438779645557588 2208 40-41 1.940669000807901 2208 42-43 1.8281645330646583 2208 44-45 1.7179346337829386 2208 46-47 1.7732316705278706 2208 48-49 1.866088103098491 2208 50-51 1.6303600516582009 2208 52-53 1.7992339114341362 2208 54-55 1.8149303971222608 2208 56-57 1.9334949498628617 2208 58-59 2.1463605562365373 2208 60-61 1.8679112547023067 2208 62-63 1.9582130534019981 2208 64-65 2.2281118668323074 2208 66-67 1.983420336924027 2208 68-69 1.7920347210957175 2208 70-71 1.7443262494118805 2208 72-73 1.6629268789401266 2208 74-75 1.7351777570902023 2208 76-77 1.900143621737918 2208 78-79 1.860088514090485 2208 80-81 2.0075899735844054 2208 82-83 1.7817158456094653 2208 84-85 1.7523062244855012 2208 86-87 1.6779761897724939 2208 88-89 1.8239242249675094 2208 90-91 1.4358143252236246 2208 92-93 1.6157557652498156 2208 94-95 1.5023190960594697 2208 96-97 1.4197809579371352 2208 98-99 1.7611466651776055 2208 100-101 1.7222165114710002 2208 102-103 1.896929313775214 2208 104-105 2.152715283245705 2208 106-107 2.1970357405753624 2208 108-109 2.065240397159453 2208 110-111 2.029546757554961 2208 112-113 2.5903792424689307 2208 114-115 2.57727400315877 2208 116-117 2.412318058337128 2208 118-119 2.4873261664531547 2208 120-121 2.4410583227271303 2209 1 0.6187139799017345 2209 2 0.9868237680455039 2209 3 0.9302419346242878 2209 4 1.0052593704769706 2209 5 1.0829766725110375 2209 6 1.2076120064106277 2209 7 1.051656527906907 2209 8 1.0941377158450223 2209 9 1.0770875684316366 2209 10-11 1.0958322104072877 2209 12-13 1.1757101956929006 2209 14-15 1.255213595528609 2209 16-17 1.4829498226697666 2209 18-19 1.3831370692221725 2209 20-21 1.2730051166597676 2209 22-23 1.6460368330150246 2209 24-25 1.4521070711498822 2209 26-27 1.4223887502456378 2209 28-29 1.2783757410414935 2209 30-31 1.4600644053995069 2209 32-33 1.204874915280847 2209 34-35 1.3648391319560105 2209 36-37 1.2401467676610949 2209 38-39 1.3298141526330056 2209 40-41 1.107271371440497 2209 42-43 1.1538069260873982 2209 44-45 1.251132189020801 2209 46-47 1.6617291100328728 2209 48-49 1.4427043919314002 2209 50-51 1.4175553073673086 2209 52-53 1.3130802514769542 2209 54-55 1.5783531397641397 2209 56-57 1.4996253119630012 2209 58-59 1.3704927488825902 2209 60-61 1.3694657058789232 2209 62-63 1.3730661936959407 2209 64-65 1.3187219983744285 2209 66-67 1.469072866027446 2209 68-69 1.3421118763366024 2209 70-71 1.3272794797202714 2209 72-73 1.6519852067893837 2209 74-75 1.5708314208295064 2209 76-77 1.5046870820748275 2209 78-79 1.1859786359503808 2209 80-81 1.3171299921924309 2209 82-83 1.3070995416480713 2209 84-85 1.6797613874570345 2209 86-87 1.6177724091656884 2209 88-89 1.656733734729162 2209 90-91 1.6521326586704213 2209 92-93 1.4177977316494648 2209 94-95 1.5384383387278326 2209 96-97 1.6697752847576588 2209 98-99 1.654224251580132 2209 100-101 2.1170452192720006 2209 102-103 2.1337883390473387 2209 104-105 1.8107738455863824 2209 106-107 1.9481949602484399 2209 108-109 1.9879924943448017 2209 110-111 1.9388193803036486 2209 112-113 1.9557773862046055 2209 114-115 2.12327592447555 2209 116-117 2.3382925214652417 2209 118-119 2.511212143109912 2209 120-121 2.181368532180919 2210 1 0.25580426167286774 2210 2 0.586140105659716 2210 3 0.609444541486468 2210 4 0.7949597091134848 2210 5 0.957777004758178 2210 6 0.8939068118543645 2210 7 0.8591320885739577 2210 8 0.5588811827161493 2210 9 0.6659317317438891 2210 10-11 0.6653038096661135 2210 12-13 0.9024912320484049 2210 14-15 0.9635325527837324 2210 16-17 1.0258128985115675 2210 18-19 0.8802810216852777 2210 20-21 0.6964285400831969 2210 22-23 0.5907783830757225 2210 24-25 0.649254218297024 2210 26-27 0.7894260002616136 2210 28-29 0.8137568668481592 2210 30-31 0.654745256463336 2210 32-33 0.8670083252865908 2210 34-35 0.7616156957538536 2210 36-37 0.6511907904072487 2210 38-39 0.9301687625620758 2210 40-41 0.8166573531669101 2210 42-43 0.7863607565135666 2210 44-45 0.8045952265263878 2210 46-47 0.7830089004615957 2210 48-49 0.8015696401583554 2210 50-51 0.6090414743853927 2210 52-53 0.7772007549591606 2210 54-55 0.4740019800938384 2210 56-57 0.6695633350925192 2210 58-59 0.6409994257722431 2210 60-61 0.8535178431225461 2210 62-63 1.002073924831329 2210 64-65 0.7550104144500764 2210 66-67 0.9234144416456189 2210 68-69 0.8321252940096002 2210 70-71 0.63896243927557 2210 72-73 0.8042438484097261 2210 74-75 1.084402438655843 2210 76-77 0.9136321974029222 2210 78-79 0.7426108000293539 2210 80-81 0.6360105749028016 2210 82-83 0.5587448039316314 2210 84-85 0.8396840761031328 2210 86-87 0.7638525318415574 2210 88-89 1.2047913668293475 2210 90-91 1.0665662411891113 2210 92-93 1.4173153343585554 2210 94-95 1.2332469771534882 2210 96-97 0.8626351659154103 2210 98-99 0.7829859920439972 2210 100-101 0.9035061482861195 2210 102-103 1.2039947156366928 2210 104-105 1.5121763118824667 2210 106-107 1.5828093618841166 2210 108-109 1.5106715563855637 2210 110-111 1.3611150802589265 2210 112-113 1.1886794371917624 2210 114-115 1.3161006641088022 2210 116-117 1.6552115681715236 2210 118-119 2.0140362438063555 2210 120-121 1.5860455496238934 2211 1 0.394308392354624 2211 2 0.5029176223975256 2211 3 0.5075330671253724 2211 4 0.8190731842907866 2211 5 0.701476198475568 2211 6 0.7387652436291816 2211 7 0.6217320466604974 2211 8 0.7337750203345834 2211 9 0.858755706242853 2211 10-11 0.6708419012801201 2211 12-13 0.6655114277180871 2211 14-15 0.9826175157785642 2211 16-17 0.766993975687484 2211 18-19 0.7093248433104193 2211 20-21 0.7202750927011294 2211 22-23 0.46238311709070246 2211 24-25 0.9355683707076068 2211 26-27 0.9426858747418336 2211 28-29 0.9240389003127447 2211 30-31 0.7406502011485969 2211 32-33 0.7978687644955329 2211 34-35 1.0418446756023059 2211 36-37 0.9935630922282783 2211 38-39 0.954360549729941 2211 40-41 0.9394604812345833 2211 42-43 1.0613373180656893 2211 44-45 1.058210262428581 2211 46-47 1.2326884830917777 2211 48-49 0.9471108201325436 2211 50-51 0.7711682515712397 2211 52-53 0.845810882574618 2211 54-55 0.9809353487227099 2211 56-57 0.9533659888763069 2211 58-59 0.5076197670360401 2211 60-61 0.9075123061339241 2211 62-63 0.8503218319158137 2211 64-65 1.0113520312066342 2211 66-67 0.8511501221326938 2211 68-69 1.0969841669787286 2211 70-71 1.0281432517348996 2211 72-73 1.009090367938093 2211 74-75 0.9608878847584421 2211 76-77 0.8946641509010398 2211 78-79 1.0420901907400086 2211 80-81 1.0867137283391557 2211 82-83 1.0641108297635604 2211 84-85 1.0885165656523412 2211 86-87 1.2744422983394657 2211 88-89 1.3064623902898553 2211 90-91 1.3989907142376516 2211 92-93 1.2983186102263318 2211 94-95 1.3219921102287238 2211 96-97 1.2383551398849484 2211 98-99 1.1336527469495294 2211 100-101 1.423881604646489 2211 102-103 1.3114652383416576 2211 104-105 1.2574285095816364 2211 106-107 1.0524629140498298 2211 108-109 1.3314848981015892 2211 110-111 1.3501315478366607 2211 112-113 1.2645461082703697 2211 114-115 1.4621062010974306 2211 116-117 1.266141054290447 2211 118-119 1.2201170044253509 2211 120-121 1.0334904830586993 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1.1178572543846315 2312 112-113 1.0717101292925229 2312 114-115 0.827952583893591 2312 116-117 1.0839854126322699 2312 118-119 1.1430821596782081 2312 120-121 1.138566031404281 2313 1 -0.3332084149340453 2313 2 -2.410553924607779 2313 3 -0.6147499076116496 2313 4 -1.69667484720528 2313 5 -2.3414635157086785 2313 6 -0.44105437650772217 2313 7 0.12511187301579696 2313 8 -0.05929327420014374 2313 9 -0.05481992961759374 2313 10-11 -0.47109396773937107 2313 12-13 -0.3653830629200385 2313 14-15 0.040336316674952855 2313 16-17 0.11536865882875702 2313 18-19 0.061091598554661175 2313 20-21 -4.645434976977178 2313 22-23 -2.050523900947283 2313 24-25 -0.12318672658486918 2313 26-27 -0.10238306881752379 2313 28-29 0.24803536129167014 2313 30-31 0.22121926104254896 2313 32-33 0.2804583727540617 2313 34-35 0.5653014052529315 2313 36-37 0.09401342959862546 2313 38-39 0.511572682816734 2313 40-41 0.46099615922040726 2313 42-43 0.03694991159259331 2313 44-45 0.07968318095762328 2313 46-47 0.5521815703672175 2313 48-49 0.26422670164269135 2313 50-51 0.10879576041878636 2313 52-53 0.219254141902816 2313 54-55 0.052115287738892846 2313 56-57 -0.29844638177797833 2313 58-59 -0.042526569648543955 2313 60-61 -0.09730027269807806 2313 62-63 -0.0019929205364022096 2313 64-65 -0.32574431833598183 2313 66-67 0.14613181473993464 2313 68-69 0.3831933121030815 2313 70-71 -0.047587513256960534 2313 72-73 -0.34897835519780074 2313 74-75 -0.21362626520298278 2313 76-77 -0.009920044737022238 2313 78-79 -0.6631914002275288 2313 80-81 -0.4257585656567535 2313 82-83 -0.030815144691811014 2313 84-85 0.2410480678382072 2313 86-87 0.04277418259265886 2313 88-89 0.3426745968511291 2313 90-91 0.3315059295791265 2313 92-93 0.07430561748805786 2313 94-95 -0.17515616128399003 2313 96-97 -0.2962635887615299 2313 98-99 -0.19577929527380888 2313 100-101 0.0931009994616403 2313 102-103 -0.06247091233002422 2313 104-105 -0.11228898101975204 2313 106-107 -0.11488288137271141 2313 108-109 -0.04793122464978694 2313 110-111 -0.1662945919363068 2313 112-113 0.17612568726156752 2313 114-115 0.22282429174045504 2313 116-117 -0.11648804650430478 2313 118-119 -0.23694940426423017 2313 120-121 -3.531260875178077E-5 2314 1 -0.32644434148448553 2314 2 -4.642783245647951 2314 3 -1.7467788976028338 2314 4 -0.7035201359449701 2314 5 -2.525621119780272 2314 6 -0.2736000504399385 2314 7 0.13243671870674234 2314 8 0.13292011317358998 2314 9 -0.2819984330905143 2314 10-11 -0.10496190394282223 2314 12-13 0.03190086573426498 2314 14-15 0.06256416810511212 2314 16-17 -0.1325758208519474 2314 18-19 0.12572543361800825 2314 20-21 0.0857540148703535 2314 22-23 0.1671330766063548 2314 24-25 0.1046569687881842 2314 26-27 0.02736995935258335 2314 28-29 -0.15304634902499714 2314 30-31 -0.2450763935526865 2314 32-33 -0.011549269218001257 2314 34-35 -0.014900627449755177 2314 36-37 0.3046066106081682 2314 38-39 0.015308586162795734 2314 40-41 -0.005966620972344572 2314 42-43 -0.002543923780152113 2314 44-45 0.19969617540403561 2314 46-47 0.2858360263794708 2314 48-49 0.20825776455746592 2314 50-51 -0.009105636484200375 2314 52-53 0.10386268420437972 2314 54-55 0.19961192310544362 2314 56-57 0.15026809620107429 2314 58-59 -0.29025640891961757 2314 60-61 0.15714336389056882 2314 62-63 0.29268129012832844 2314 64-65 0.2993484991384534 2314 66-67 0.5964822007059354 2314 68-69 0.6275063080964571 2314 70-71 0.7380079881894481 2314 72-73 0.3417112786372627 2314 74-75 0.24885227075605343 2314 76-77 0.1926579606154526 2314 78-79 0.09601686671552656 2314 80-81 0.4373356595493796 2314 82-83 0.76161791070475 2314 84-85 0.6474938546983466 2314 86-87 0.4595749189446252 2314 88-89 0.8609925121706503 2314 90-91 0.7817190230308562 2314 92-93 0.700574122972025 2314 94-95 0.4825774680339556 2314 96-97 0.4574839167688438 2314 98-99 0.6852557742619467 2314 100-101 0.4201066303884211 2314 102-103 0.789307315784388 2314 104-105 0.8585431276385727 2314 106-107 1.0781987361264775 2314 108-109 1.129736891000789 2314 110-111 1.0338372862252925 2314 112-113 0.7540614266300985 2314 114-115 0.889292432142355 2314 116-117 0.7938522068874043 2314 118-119 0.28426712343405214 2314 120-121 0.6996962901943355 2315 1 0.2077785260591014 2315 2 0.430255816556361 2315 3 0.13871134974869292 2315 4 0.5248525187916613 2315 5 0.6368519137768232 2315 6 0.4289243870925361 2315 7 0.3998681370642245 2315 8 0.42880951549054913 2315 9 -0.08797767194008088 2315 10-11 0.17121231906234868 2315 12-13 0.029894940858383734 2315 14-15 -0.7017293368959372 2315 16-17 -0.18994768078815127 2315 18-19 0.3134789697917455 2315 20-21 0.33799304679656217 2315 22-23 0.23249939014308296 2315 24-25 0.10144336015341793 2315 26-27 0.04173313841664594 2315 28-29 0.3400017277988283 2315 30-31 0.3303212647106548 2315 32-33 0.35785057044564184 2315 34-35 0.0874755161510663 2315 36-37 0.06877969130277961 2315 38-39 0.2599075560188737 2315 40-41 0.3294804456635845 2315 42-43 0.22968402910985475 2315 44-45 0.004300856812932352 2315 46-47 0.12503172964022014 2315 48-49 0.2550219634433688 2315 50-51 0.3491738786531329 2315 52-53 0.1469986707719002 2315 54-55 0.4422634598916275 2315 56-57 0.49477107068143766 2315 58-59 0.30519189636631694 2315 60-61 0.30067537439156666 2315 62-63 -0.2676358042740077 2315 64-65 -0.20253700143098285 2315 66-67 -0.13515395273527986 2315 68-69 -0.15466160467505574 2315 70-71 -0.05425836285084884 2315 72-73 -0.16886541616281647 2315 74-75 0.325426646528058 2315 76-77 0.20298520506647222 2315 78-79 0.1372140219493403 2315 80-81 0.12825635498254329 2315 82-83 0.2090286160815218 2315 84-85 -0.026032409842891013 2315 86-87 -0.22684190742761245 2315 88-89 -0.12334888729741778 2315 90-91 -0.17727994132011204 2315 92-93 0.045500302872177656 2315 94-95 -0.3528369120045092 2315 96-97 -0.20445752949266804 2315 98-99 -1.7611880959688193 2315 100-101 -10.697460179296446 2315 102-103 -4.449559457085048 2315 104-105 -1.2284479506406676 2315 106-107 -9.630418867949686E-4 2315 108-109 0.3702534768684771 2315 110-111 0.2954023205118119 2315 112-113 0.5179214041279572 2315 114-115 0.383303903417719 2315 116-117 0.5050196689279396 2315 118-119 0.5368691486268169 2315 120-121 0.16597748439057014 2316 1 -1.3743286425526513 2316 2 -3.233765729014582 2316 3 -1.3822116954764034 2316 4 -4.261599084616776 2316 5 -4.43062516462021 2316 6 -1.6773906432979047 2316 7 -7.830575283736671 2316 8 -3.589065655593643 2316 9 -2.100442903216482 2316 10-11 -0.9065121507606122 2316 12-13 -0.6470230417462162 2316 14-15 -4.977620078481536 2316 16-17 -3.6245059524330685 2316 18-19 -5.5192779508399035 2316 20-21 -1.456792748432214 2316 22-23 -3.763962662278594 2316 24-25 -1.0851995632155749 2316 26-27 -3.655570721831481 2316 28-29 -2.274824097452459 2316 30-31 -0.9644453985220665 2316 32-33 -0.18000281959689346 2316 34-35 -0.6793819799121579 2316 36-37 -0.4518725625935289 2316 38-39 -0.19861721991601655 2316 40-41 -0.44490769838562727 2316 42-43 -0.15364758724947336 2316 44-45 -0.7126942424526916 2316 46-47 -0.2866078824217695 2316 48-49 -0.2239622747352641 2316 50-51 -0.5030152632032525 2316 52-53 -0.6981911039120448 2316 54-55 -0.49982840312328136 2316 56-57 -0.45571826690998307 2316 58-59 -0.408443330053494 2316 60-61 -0.17120353479032246 2316 62-63 -0.30021465017313886 2316 64-65 -0.39467669443602915 2316 66-67 -0.37594189663438016 2316 68-69 -0.47325643314935206 2316 70-71 -0.15510752913732873 2316 72-73 0.12465030886625073 2316 74-75 -0.12801784860799614 2316 76-77 -0.601917000999844 2316 78-79 -0.5984524337748063 2316 80-81 -0.42760465842456696 2316 82-83 -0.18070927557251082 2316 84-85 -0.2839287732919473 2316 86-87 -0.5569115184594153 2316 88-89 -0.4343468416455316 2316 90-91 -0.5335589152564459 2316 92-93 -0.41300828445066884 2316 94-95 -0.34168001491993394 2316 96-97 -0.6177748704395576 2316 98-99 -0.03548352342176386 2316 100-101 0.026362099177113407 2316 102-103 -0.3670166676400193 2316 104-105 -0.22513222855498327 2316 106-107 0.18718983747860918 2316 108-109 -0.42494439212149615 2316 110-111 -0.08740390180198432 2316 112-113 -0.542818268273404 2316 114-115 -0.02724518486908778 2316 116-117 0.33493622399129563 2316 118-119 0.3370531101273535 2316 120-121 -0.2971536875177705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8593.0 3 1445.0 4 1243.0 5 1250.0 6 1520.0 7 1659.0 8 1825.0 9 2108.0 10 2478.0 11 2970.0 12 3203.0 13 3730.0 14 4527.0 15 4875.0 16 3967.0 17 3941.0 18 4243.0 19 4513.0 20 4940.0 21 5469.0 22 5921.0 23 6649.0 24 7294.0 25 8222.0 26 9241.0 27 10623.0 28 12222.0 29 14207.0 30 16681.0 31 20105.0 32 24045.0 33 30090.0 34 38354.0 35 49862.0 36 65370.0 37 71191.0 38 26874.0 39 134.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.42152125612244 17.599796813440047 14.38370873289227 21.594973197545244 2 13.294199254674947 20.630797464478324 37.178971751388886 28.89603152945784 3 23.881143770579836 22.991345541434416 32.14471553514859 20.982795152837156 4 10.3174206760486 14.573629859021263 45.0501354844349 30.058813980495238 5 10.377574465959608 37.8706648240955 37.8746191372105 13.877141572734386 6 25.73609755386879 34.90393841069113 19.732430334274767 19.62753370116531 7 21.8269178746374 33.45444254387608 25.43959313964183 19.279046441844688 8 20.501657555142582 27.57701440293678 22.891741775176005 29.02958626674464 9 18.93482504443085 20.219261553061045 29.942021385077062 30.903892017431044 10-11 23.63261029484446 21.85898616456643 29.093116827578886 25.41528671301022 12-13 28.001723118928503 21.58383348178239 27.9861151576196 22.428328241669508 14-15 23.465873382528603 23.039056549588583 32.42028659782659 21.07478347005623 16-17 18.96662240739893 28.06117352269085 31.713603689474755 21.25860038043546 18-19 16.993574920753193 31.827765416964127 30.509344150721912 20.66931551156077 20-21 19.335345231281543 27.18684634996618 26.71252406472761 26.765284354024665 22-23 17.405650756916724 26.37793391078497 30.092694723435077 26.12372060886323 24-25 22.961516087775912 24.765881989329277 28.355570078266545 23.917031844628266 26-27 21.035224181318256 22.360475983323518 34.835680848893816 21.768618986464404 28-29 18.173255259668487 28.9099106787871 31.72651597311413 21.190318088430292 30-31 18.35361880793434 26.196260145339334 30.170714394256372 25.279406652469955 32-33 19.908742619424043 25.192782257267126 31.787710020514176 23.11076510279465 34-35 23.537555022862655 23.868550914558302 29.277107054271877 23.316787008307163 36-37 21.343077424304088 22.87401414981904 34.4395187354471 21.343389690429774 38-39 21.589924972312204 26.03964559618283 31.61696741583326 20.753462015671712 40-41 19.34690444740751 28.611547417189453 31.942705291985046 20.098842843417987 42-43 18.143307083335934 28.29056519720805 27.465907269438215 26.100220450017797 44-45 18.925714880441287 26.027840657768063 27.53081986834231 27.51562459344834 46-47 22.483502987156267 27.03761527092571 29.133412435729305 21.345469306188722 48-49 21.542589452890418 25.21271048580733 29.897284903514006 23.347415157788248 50-51 17.015311354472377 26.656144686938134 34.77260967654282 21.555934282046664 52-53 18.95571912703898 28.58272896075672 28.860004829429542 23.601547082774758 54-55 17.287645139297293 25.09943115283929 31.752913194860078 25.860010513003335 56-57 21.65706464321587 23.4737987061989 29.03526987150463 25.8338667790806 58-59 24.089361241549273 24.19614014054018 28.80126636027575 22.913232257634792 60-61 22.00825266524853 24.213752984380744 33.51866949460495 20.259324855765776 62-63 19.580226658032867 26.858747863238964 31.71848680881897 21.8425386699092 64-65 17.78676782903449 30.467598337650227 29.891168558602825 21.85446527471246 66-67 19.12997128776159 26.712896354857445 26.778170370385794 27.378961986995165 68-69 17.614456752685552 26.843555558331587 29.81783317353787 25.724154515444987 70-71 22.466406949855212 25.00895814670528 27.41067894418866 25.11395595925085 72-73 20.617808159242955 22.86804827791679 33.33972260824091 23.17442095459934 74-75 18.040987944477088 28.592070774628812 30.429985873410757 22.936955407483342 76-77 18.494648525935347 25.809701519749872 29.31626362612507 26.379386328189707 78-79 20.25438417405869 24.9423984734033 31.057326539200286 23.745890813337724 80-81 22.750631432753714 24.97298321523684 28.221244042252376 24.055141309757065 82-83 20.022473758918427 23.75553382467344 33.39925497093928 22.822737445468853 84-85 20.55791211926225 25.75564482038403 30.509205175138842 23.177237885214872 86-87 19.584180494523512 27.671329566784408 30.771184755575128 21.97330518311695 88-89 18.840133094019514 25.551782152241902 27.898626166550756 27.709458587187825 90-91 18.75454214170441 23.720339424228225 29.119787599562706 28.40533083450466 92-93 21.270231608054953 24.896564504811025 30.913626416186535 22.919577470947484 94-95 19.93133265248971 23.687111755171134 31.413437604429856 24.9681179879093 96-97 15.071375547510993 26.07209081264322 34.97146748042391 23.885066159421875 98-99 17.60752954113995 27.995430456610688 29.159372718742937 25.237667283506426 100-101 16.238364834231103 24.990133733468145 31.42210647654015 27.349394955760598 102-103 19.59134127386506 23.334065547385528 30.060163009587143 27.014430169162267 104-105 22.488422275448354 23.788853948397023 29.59140443081522 24.1313193453394 106-107 21.621509087899838 23.924894318878536 32.33118865628575 22.12240793693588 108-109 18.980835230448783 25.45090973061481 30.875775110886266 24.69247992805014 110-111 18.727787181681336 27.49611178083561 29.028184644084803 24.74791639339825 112-113 20.623874413133322 24.10926390522689 26.192523500692268 29.074338180947525 114-115 18.396931068764673 24.40123048735419 28.953304982849172 28.248533461031965 116-117 22.908611919550584 23.353447423088337 26.827267000223877 26.910673657137202 118-119 20.341579014224997 22.38984529717145 31.890773014362438 25.377802674241114 120-121 17.815530746263686 27.842160712575424 29.789958483070507 24.55235005809039 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1411.0 1 1638.0 2 1087.5 3 295.0 4 279.5 5 279.0 6 272.5 7 269.0 8 292.5 9 315.0 10 300.5 11 293.0 12 320.0 13 366.0 14 395.0 15 407.0 16 453.0 17 488.0 18 527.5 19 594.0 20 664.0 21 761.0 22 884.0 23 1010.5 24 1198.5 25 1553.5 26 1957.5 27 2487.0 28 3416.0 29 4546.0 30 5897.5 31 7263.0 32 8424.0 33 9377.5 34 10016.0 35 10574.5 36 11094.5 37 11735.0 38 12266.0 39 12475.0 40 12704.0 41 12950.0 42 13173.5 43 13582.5 44 14039.0 45 14771.0 46 17856.0 47 31393.5 48 60951.5 49 60725.5 50 31440.5 51 16963.5 52 11407.0 53 8949.0 54 7605.5 55 6489.0 56 5369.0 57 4337.5 58 3310.5 59 2618.5 60 2109.0 61 1592.0 62 1180.5 63 844.5 64 598.0 65 429.0 66 311.5 67 208.5 68 135.5 69 99.0 70 63.5 71 45.0 72 31.0 73 22.5 74 19.5 75 15.5 76 14.0 77 10.5 78 8.5 79 8.0 80 3.0 81 2.5 82 3.0 83 2.5 84 2.5 85 1.5 86 2.0 87 1.5 88 1.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.26771887047349174 2 1.1378051995123397 3 1.058107351148308 4 1.0473985963293684 5 1.0494579722560875 6 1.0525470361461664 7 1.0350423407690534 8 1.0414264061418828 9 1.0424560941052423 10-11 1.03844031104814 12-13 1.0420442189198984 14-15 1.0399848429931793 16-17 1.0465748459586806 18-19 1.0550182872582292 20-21 1.0521351609608225 22-23 1.0465748459586806 24-25 1.0524440673498303 26-27 1.0599607894823553 28-29 1.0673745428185442 30-31 1.0690220435599196 32-33 1.067683449207552 34-35 1.0740675145803815 36-37 1.0759209529144287 38-39 1.0766417344887804 40-41 1.0761268905071006 42-43 1.0714932946719826 44-45 1.0650062605028172 46-47 1.0696398563379352 48-49 1.0662418860588487 50-51 1.095485024218261 52-53 1.0700517315232794 54-55 1.0763328280997726 56-57 1.0742734521730535 58-59 1.0609904774457148 60-61 1.1070175294078883 62-63 1.0916751787538304 64-65 1.0914692411611586 66-67 1.0916751787538304 68-69 1.0878653332894 70-71 1.1478961415532638 72-73 1.1559277076674683 74-75 1.1610761474842664 76-77 1.1675631816534318 78-79 1.1454248904412008 80-81 1.0970295561633001 82-83 1.1261697255263765 84-85 1.1048551846848331 86-87 1.0921900227355101 88-89 1.0959998681999408 90-91 1.1036195591288016 92-93 1.0982651817193319 94-95 1.0909543971794788 96-97 1.100221588849715 98-99 1.122050973672938 100-101 1.1146372203367492 102-103 1.092601897920854 104-105 1.0798337671751952 106-107 1.0806575175458828 108-109 1.0810693927312267 110-111 1.0887920524564236 112-113 1.0871445517150482 114-115 1.0886890836600878 116-117 1.1134015947807177 118-119 1.1065026854262086 120-121 1.0910573659758147 >>END_MODULE >>Sequence Length Distribution pass #Length Count 121 485584.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.0066912747096 #Duplication Level Percentage of deduplicated Percentage of total 1 91.19653361937566 54.724022382215495 2 5.490739342230714 6.589622011582811 3 1.2783621872546813 2.301308553235625 4 0.5975551743173209 1.4342923545945903 5 0.3544996140674939 1.0636174449175904 6 0.22754501450326672 0.8192534061838107 7 0.15400631442791643 0.6468986554962285 8 0.11565530504344378 0.5552073747219435 9 0.08138352532732324 0.43952004712477755 >10 0.43981305911113355 4.7488425322378545 >50 0.02521729533956242 1.0733401410536825 >100 0.02452641052774365 2.903076220892181 >500 0.005872520830586508 2.5958745815232263 >1k 0.006563405634184921 8.09606911527211 >5k 6.908848035984126E-4 3.4563293757341107 >10k+ 0.001036327205397619 8.552725803213951 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 19125 3.9385564598504073 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 11794 2.4288279679725857 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10341 2.1296006458202905 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 9976 2.0544334244950413 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 6698 1.379369995716498 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4542 0.9353685459158456 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3003 0.6184305907937658 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2742 0.5646808791063956 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2642 0.544087119839204 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2641 0.5438811822465319 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2267 0.46686052258723515 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 2199 0.45285676628554483 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 2119 0.4363817588717915 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2003 0.41249299812184914 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1992 0.41022768460245806 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1946 0.4007545553395499 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1923 0.39601799070809585 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1605 0.3305298362384263 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1523 0.31364295363932915 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1460 0.3006688853009984 No Hit GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1252 0.25783386602523967 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 1145 0.23579854360934466 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 1050 0.21623447230551254 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1003 0.20655540544993245 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 995 0.20490790470855713 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 967 0.19914165211374346 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 959 0.19749415137236812 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 889 0.18307851988533394 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 884 0.18204883192197435 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 791 0.1628966358034861 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 788 0.16227882302547036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 751 0.15465913209660945 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 721 0.14848100431645195 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 689 0.1418910013509506 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 638 0.13138818412468284 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 627 0.12912287060529176 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 604 0.12438630597383768 No Hit GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC 577 0.11882599097169594 No Hit CNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 566 0.11656067745230486 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 560 0.11532505189627335 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 517 0.10646973541138094 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.118751853438334E-4 0.0 12-13 0.0 0.0 0.0 7.207815743517085E-4 0.0 14-15 0.0 0.0 0.0 0.001956407130383209 0.0 16-17 0.0 0.0 0.0 0.0022653135193910837 0.0 18-19 0.0 0.0 0.0 0.0029860950937427924 0.0 20-21 0.0 0.0 0.0 0.004015783057102375 0.0 22-23 0.0 0.0 0.0 0.006795940558173251 0.0 24-25 0.0 0.0 0.0 0.009164222873900292 0.0 26-27 0.0 0.0 0.0 0.012047349171307127 0.0 28-29 0.0 0.0 0.0 0.020387821674519753 0.0 30-31 0.0 0.0 0.0 0.034700484365217964 0.0 32-33 0.0 0.0 0.0 0.05632393159576922 0.0 34-35 0.0 0.0 0.0 0.08072753632739135 0.0 36-37 0.0 0.0 0.0 0.09761441892648852 0.0 38-39 0.0 0.0 0.0 0.10914692411611585 0.0 40-41 0.0 0.0 0.0 0.12675458828956473 0.0 42-43 0.0 0.0 0.0 0.1621758542291344 0.0 44-45 0.0 0.0 0.0 0.21345431480444166 0.0 46-47 0.0 0.0 0.0 0.3343396817028568 0.0 48-49 0.0 0.0 0.0 0.7459059606576823 0.0 50-51 0.0 0.0 0.0 0.9059194701637616 0.0 52-53 0.0 0.0 0.0 1.0399848429931793 0.0 54-55 0.0 0.0 0.0 1.1883628785132954 0.0 56-57 0.0 0.0 0.0 1.2946266763320042 0.0 58-59 0.0 0.0 0.0 1.4132467297110285 0.0 60-61 0.0 0.0 0.0 1.767356420310389 0.0 62-63 0.0 0.0 0.0 1.8838141289663581 0.0 64-65 0.0 0.0 0.0 2.0291031005963953 0.0 66-67 0.0 0.0 0.0 2.6067580480411214 0.0 68-69 0.0 0.0 0.0 3.139106725098026 0.0 70-71 0.0 0.0 0.0 3.4602664008698802 0.0 72-73 0.0 0.0 0.0 3.7851329533098292 0.0 74-75 0.0 0.0 0.0 3.972433193844937 0.0 76-77 0.0 0.0 0.0 4.054602293321032 0.0 78-79 0.0 0.0 0.0 4.221102836996277 0.0 80-81 0.0 0.0 0.0 4.539894230452404 0.0 82-83 0.0 0.0 0.0 4.970509736729381 0.0 84-85 0.0 0.0 0.0 5.640115819302118 0.0 86-87 0.0 0.0 0.0 7.419416619987479 0.0 88-89 0.0 1.0296879633595835E-4 0.0 9.515964282183926 0.0 90-91 0.0 2.059375926719167E-4 0.0 10.913971630037233 0.0 92-93 0.0 2.059375926719167E-4 0.0 12.250918481663316 0.0 94-95 0.0 2.059375926719167E-4 0.0 13.414980724241325 0.0 96-97 0.0 2.059375926719167E-4 0.0 14.438593528617087 0.0 98-99 0.0 2.059375926719167E-4 0.0 15.106449141652114 0.0 100-101 0.0 2.059375926719167E-4 0.0 15.44336304326337 0.0 102-103 0.0 2.059375926719167E-4 0.0 15.76586131338759 0.0 104-105 0.0 2.059375926719167E-4 0.0 16.164041648818742 0.0 106-107 0.0 2.059375926719167E-4 0.0 16.69577251309763 0.0 108-109 0.0 2.059375926719167E-4 0.0 17.423864871989192 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGCG 20 9.749335E-4 86.166954 9 GTACCCG 60 3.9407496E-7 57.600636 1 TACCTGG 325 0.0 54.867645 2 TACCCGG 70 1.1526226E-6 49.305027 2 ACCTGGG 390 0.0 44.197388 3 TACACGG 60 0.0015440759 38.34835 2 ACACGGG 60 0.0015528019 38.3044 3 TCGCAAC 45 2.943628E-4 31.908699 102-103 TAGACAG 180 3.0737283E-8 28.73429 5 TATGTAC 225 3.074092E-10 28.089895 4 ACCCGGG 125 6.187883E-5 27.57917 3 GTACCTG 675 0.0 27.306969 1 CCTGGGG 350 0.0 26.265877 4 TCTCGGG 190 0.0 25.714989 114-115 CATGGGG 400 0.0 24.419058 4 GTATTGG 145 1.6680114E-4 23.834745 1 GCTTATG 220 2.0996595E-7 23.563898 1 GAGTACT 1245 0.0 23.546255 12-13 TCGCCGT 285 0.0 23.172173 104-105 ATACTGC 125 0.001997679 22.987432 6 >>END_MODULE